RchiOBHm_Chr5g0037961

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000691
Physical Location & Seq
Forward (+)
32622361 .. 32623802
1442 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31663

Sequence Viewer

Length: 1002 bp
ATGATCAGATTGGCAAATAGAAGATGTGCAGGGCTTAGATGGAAAAGTAAGGTCGGTCCAAGAATTGCAAAACTGTTGAAGAAAAATGCTGATAGAAGCCATGAGTACGCACCAAGAGAATCTTCAAACAATAGGTTCCAAATTCAAGGAAGAGGAGTCTCATGTCAATCAGGTGTTATAAGCACTCATGATGTAGATCTCAATAAAAGAAATTGTACCTGCAGAAGATGGAATATCAGTGGACTTCCATGTCCACATGCAATTTGTGCAATCTTTTCAAAGGGCCTGAAGCCAGAAGACTTTGTGGATGTGGCCTATTTGCAAAAAAAATACATGCTTGCATATGGTCCAGCCATAAACCCAATTCCTGGTGTTGAAGAATGGGATGTTATCGAAAAGCCAATTCTGCCACCACAGTACACTAGAGGACCTGGAAGGCCTAAATTCTCAAGAAACAAAGAACCATATGAGCAACGTCTACCACCTCCTGGAACCACTAAGCCACCTTCTGGAACTGAGAAGTTACCAAGGGCTTACTATCAAGGAATTACATGTGGACGATGCCATAAGAAAGGGCATAATAGCAGAACTTGTGCAAGAAGAGAAGCACAGTCCCAAACAAGGATTGAATCACCACCTTCTCAAAACTATGGAAATGATGTGCATGTTGATCAAGCTTCACATGATGACAATGTTCAACCAAACACCACCACCTCTATGCAGCAAGATCAAGCCCATACACATATATCATTTGAGAACACACTGATAGAAGAGGTGCATATACAAACACCCTCAAGTGTGGGAGCTACTTCATCACAACCACTTTTTACAAACCCATCTATGTCACAAGTTTACCCTCATCCTCAATCGTCTTCACAGCCAAGTGTTATCGGATCATCCTCTGAGAAAGGGAGGCTAATGCGGACTAAGTCACCAGCAAAGAAGCAAATACCTATGAGAATTCAGCGCAACAAAGCCTTTTCACCAAATGAAAGATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

333

Amino Acids

37.35

Weight (kDa)

9.83

Isoelectric Point (pI)

69.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIM PF04434 64 - 93 8e-07 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 179
AasI GACNNNNNNGTC 1 cut(s) 249
Acc36I ACCTGC 1 cut(s) 227
AccB7I CCANNNNNTGG 3 cut(s) 368, 488, 509
AccI GTMKAC 1 cut(s) 478
AciI CCGC 1 cut(s) 922
AclWI GGATC 1 cut(s) 901
AcsI RAATTY 3 cut(s) 141, 443, 960
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 3 cut(s) 107, 217, 419
AfiI CCNNNNNNNGG 4 cut(s) 368, 488, 509, 621
AflIII ACRYGT 1 cut(s) 551
AgsI TTSAA 7 cut(s) 79, 126, 146, 279, 377, 629, 698
AjnI CCWGG 3 cut(s) 367, 430, 487
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AluBI AGCT 2 cut(s) 677, 806
AluI AGCT 2 cut(s) 677, 806
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 1 cut(s) 901
AoxI GGCC 3 cut(s) 283, 312, 437
ApeKI GCWGC 1 cut(s) 721
ApoI RAATTY 3 cut(s) 141, 443, 960
AspLEI GCGC 1 cut(s) 969
AspS9I GGNCC 4 cut(s) 56, 283, 347, 428
AsuHPI GGTGA 3 cut(s) 624, 924, 975
AvaII GGWCC 3 cut(s) 56, 347, 428
BbsI GAAGAC 2 cut(s) 303, 864
BbvI GCAGC 1 cut(s) 733
BccI CCATC 3 cut(s) 33, 222, 844
BciT130I CCWGG 3 cut(s) 369, 432, 489
BclI TGATCA 2 cut(s) 3, 670
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 1 cut(s) 423
BfmI CTRYAG 1 cut(s) 220
BfuAI ACCTGC 1 cut(s) 227
BglII AGATCT 2 cut(s) 196, 995
BisI GCNGC 1 cut(s) 722
BlsI GCNGC 1 cut(s) 723
Bme1390I CCNGG 3 cut(s) 369, 432, 489
Bme18I GGWCC 3 cut(s) 56, 347, 428
BmgT120I GGNCC 4 cut(s) 56, 283, 347, 428
BmiI GGNNCC 2 cut(s) 137, 493
BmrFI CCNGG 3 cut(s) 369, 432, 489
BmsI GCATC 1 cut(s) 551
BpiI GAAGAC 2 cut(s) 303, 864
BpuEI CTTGAG 2 cut(s) 433, 778
BsaBI GATNNNNATC 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 527
Bsc4I CCNNNNNNNGG 4 cut(s) 368, 488, 509, 621
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 3 cut(s) 369, 432, 489
BseDI CCNNGG 1 cut(s) 527
BseGI GGATG 4 cut(s) 313, 391, 859, 896
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 4 cut(s) 368, 488, 509, 621
BseMII CTCAG 2 cut(s) 507, 894
BseRI GAGGAG 1 cut(s) 168
BseXI GCAGC 1 cut(s) 733
BsgI GTGCAG 1 cut(s) 48
BshFI GGCC 3 cut(s) 285, 314, 439
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 4 cut(s) 368, 488, 509, 621
BsmAI GTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 598
BsnI GGCC 3 cut(s) 285, 314, 439
Bsp143I GATC 6 cut(s) 3, 196, 670, 727, 893, 995
BspACI CCGC 1 cut(s) 922
BspANI GGCC 3 cut(s) 285, 314, 439
BspCNI CTCAG 2 cut(s) 508, 895
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 2 cut(s) 137, 493
BspMAI CTGCAG 1 cut(s) 224
BspMI ACCTGC 1 cut(s) 227
BspPI GGATC 1 cut(s) 901
BssECI CCNNGG 1 cut(s) 527
BssMI GATC 6 cut(s) 3, 196, 670, 727, 893, 995
BssT1I CCWWGG 1 cut(s) 527
Bst2UI CCWGG 3 cut(s) 369, 432, 489
Bst4CI ACNGT 3 cut(s) 75, 417, 612
Bst6I CTCTTC 3 cut(s) 145, 595, 765
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 6 cut(s) 35, 498, 516, 903, 927, 999
BstF5I GGATG 4 cut(s) 313, 391, 859, 896
BstHHI GCGC 1 cut(s) 969
BstKTI GATC 6 cut(s) 6, 199, 673, 730, 896, 998
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 6 cut(s) 3, 196, 670, 727, 893, 995
BstMWI GCNNNNNNNGC 2 cut(s) 266, 406
BstNI CCWGG 3 cut(s) 369, 432, 489
BstNSI RCATGY 4 cut(s) 260, 337, 555, 668
BstSCI CCNGG 3 cut(s) 367, 430, 487
BstSFI CTRYAG 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 733
BstV2I GAAGAC 2 cut(s) 303, 864
BstX2I RGATCY 2 cut(s) 196, 995
BstYI RGATCY 2 cut(s) 196, 995
BsuRI GGCC 3 cut(s) 285, 314, 439
BtsCI GGATG 4 cut(s) 313, 391, 859, 896
BtsIMutI CAGTG 2 cut(s) 244, 761
BveI ACCTGC 1 cut(s) 227
Cac8I GCNNGC 1 cut(s) 339
CciI TCATGA 1 cut(s) 187
CfoI GCGC 1 cut(s) 969
Cfr13I GGNCC 4 cut(s) 56, 283, 347, 428
Csp6I GTAC 3 cut(s) 106, 216, 418
CviAII CATG 9 cut(s) 101, 162, 188, 249, 257, 334, 552, 665, 683
CviQI GTAC 3 cut(s) 106, 216, 418
DdeI CTNAG 6 cut(s) 35, 498, 516, 903, 927, 999
DpnI GATC 6 cut(s) 5, 198, 672, 729, 895, 997
DpnII GATC 6 cut(s) 3, 196, 670, 727, 893, 995
DrdI GACNNNNNNGTC 1 cut(s) 249
DseDI GACNNNNNNGTC 1 cut(s) 249
Eam1104I CTCTTC 3 cut(s) 145, 595, 765
EarI CTCTTC 3 cut(s) 145, 595, 765
Eco130I CCWWGG 1 cut(s) 527
Eco147I AGGCCT 1 cut(s) 439
Eco47I GGWCC 3 cut(s) 56, 347, 428
Eco57I CTGAAG 1 cut(s) 308
EcoO109I RGGNCCY 2 cut(s) 283, 428
EcoRI GAATTC 1 cut(s) 960
EcoRII CCWGG 3 cut(s) 367, 430, 487
EcoT14I CCWWGG 1 cut(s) 527
ErhI CCWWGG 1 cut(s) 527
FaeI CATG 9 cut(s) 104, 165, 191, 252, 260, 337, 555, 668, 686
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FaqI GGGAC 1 cut(s) 598
FatI CATG 9 cut(s) 100, 161, 187, 248, 256, 333, 551, 664, 682
FauNDI CATATG 2 cut(s) 343, 466
FbaI TGATCA 2 cut(s) 3, 670
FblI GTMKAC 1 cut(s) 478
Fnu4HI GCNGC 1 cut(s) 722
FokI GGATG 4 cut(s) 320, 398, 846, 883
Fsp4HI GCNGC 1 cut(s) 722
FspBI CTAG 1 cut(s) 423
GlaI GCGC 1 cut(s) 968
GluI GCNGC 1 cut(s) 722
HaeIII GGCC 3 cut(s) 285, 314, 439
HhaI GCGC 1 cut(s) 969
Hin1II CATG 9 cut(s) 104, 165, 191, 252, 260, 337, 555, 668, 686
Hin6I GCGC 1 cut(s) 967
HinP1I GCGC 1 cut(s) 967
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 3 cut(s) 119, 156, 629
HphI GGTGA 3 cut(s) 624, 924, 975
Hpy166II GTNNAC 6 cut(s) 242, 254, 420, 479, 557, 853
Hpy188I TCNGA 3 cut(s) 8, 893, 904
Hpy188III TCNNGA 3 cut(s) 188, 450, 510
Hpy8I GTNNAC 6 cut(s) 242, 254, 420, 479, 557, 853
HpyAV CCTTC 3 cut(s) 429, 516, 648
HpyCH4III ACNGT 3 cut(s) 75, 417, 612
HpyCH4IV ACGT 1 cut(s) 475
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 406
HpyF3I CTNAG 6 cut(s) 35, 498, 516, 903, 927, 999
HpySE526I ACGT 1 cut(s) 475
Hsp92II CATG 9 cut(s) 104, 165, 191, 252, 260, 337, 555, 668, 686
HspAI GCGC 1 cut(s) 967
Ksp22I TGATCA 2 cut(s) 3, 670
Kzo9I GATC 6 cut(s) 3, 196, 670, 727, 893, 995
LmnI GCTCC 1 cut(s) 803
Lsp1109I GCAGC 1 cut(s) 733
LweI GCATC 1 cut(s) 551
MaeI CTAG 1 cut(s) 423
MaeII ACGT 1 cut(s) 475
MaeIII GTNAC 3 cut(s) 522, 843, 930
MalI GATC 6 cut(s) 5, 198, 672, 729, 895, 997
MboI GATC 6 cut(s) 3, 196, 670, 727, 893, 995
MflI RGATCY 2 cut(s) 196, 995
MluCI AATT 9 cut(s) 63, 141, 211, 261, 363, 402, 443, 546, 960
MlyI GAGTC 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 716
MspR9I CCNGG 3 cut(s) 369, 432, 489
MvaI CCWGG 3 cut(s) 369, 432, 489
MwoI GCNNNNNNNGC 2 cut(s) 266, 406
NdeI CATATG 2 cut(s) 343, 466
NdeII GATC 6 cut(s) 3, 196, 670, 727, 893, 995
NlaIII CATG 9 cut(s) 104, 165, 191, 252, 260, 337, 555, 668, 686
NlaIV GGNNCC 2 cut(s) 137, 493
NmuCI GTSAC 2 cut(s) 843, 930
NspI RCATGY 4 cut(s) 260, 337, 555, 668
PagI TCATGA 1 cut(s) 187
PceI AGGCCT 1 cut(s) 439
PciI ACATGT 1 cut(s) 551
PfeI GAWTC 2 cut(s) 119, 629
PflFI GACNNNGTC 1 cut(s) 928
PflMI CCANNNNNTGG 3 cut(s) 368, 488, 509
PfoI TCCNGGA 1 cut(s) 487
PkrI GCNGC 1 cut(s) 723
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
PpuMI RGGWCCY 1 cut(s) 428
PscI ACATGT 1 cut(s) 551
PsiI TTATAA 1 cut(s) 179
Psp5II RGGWCCY 1 cut(s) 428
Psp6I CCWGG 3 cut(s) 367, 430, 487
PspGI CCWGG 3 cut(s) 367, 430, 487
PspN4I GGNNCC 2 cut(s) 137, 493
PspPI GGNCC 4 cut(s) 56, 283, 347, 428
PspPPI RGGWCCY 1 cut(s) 428
PstI CTGCAG 1 cut(s) 224
PsuI RGATCY 2 cut(s) 196, 995
PsyI GACNNNGTC 1 cut(s) 928
RsaI GTAC 3 cut(s) 107, 217, 419
RsaNI GTAC 3 cut(s) 106, 216, 418
RseI CAYNNNNRTG 1 cut(s) 716
SatI GCNGC 1 cut(s) 722
Sau3AI GATC 6 cut(s) 3, 196, 670, 727, 893, 995
Sau96I GGNCC 4 cut(s) 56, 283, 347, 428
SchI GAGTC 1 cut(s) 165
ScrFI CCNGG 3 cut(s) 369, 432, 489
SfaNI GCATC 1 cut(s) 551
SfcI CTRYAG 1 cut(s) 220
SinI GGWCC 3 cut(s) 56, 347, 428
SmiMI CAYNNNNRTG 1 cut(s) 716
SmlI CTYRAG 2 cut(s) 448, 793
SmoI CTYRAG 2 cut(s) 448, 793
Sse9I AATT 9 cut(s) 63, 141, 211, 261, 363, 402, 443, 546, 960
SseBI AGGCCT 1 cut(s) 439
SsiI CCGC 1 cut(s) 922
SspMI CTAG 1 cut(s) 423
StuI AGGCCT 1 cut(s) 439
StyD4I CCNGG 3 cut(s) 367, 430, 487
StyI CCWWGG 1 cut(s) 527
TaaI ACNGT 3 cut(s) 75, 417, 612
TaiI ACGT 1 cut(s) 478
TaqI TCGA 1 cut(s) 393
TaqII GACCGA 1 cut(s) 44
TasI AATT 9 cut(s) 63, 141, 211, 261, 363, 402, 443, 546, 960
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 2 cut(s) 119, 629
TscAI CASTG 2 cut(s) 244, 768
TseFI GTSAC 2 cut(s) 843, 930
TseI GCWGC 1 cut(s) 721
Tsp45I GTSAC 2 cut(s) 843, 930
TspDTI ATGAA 1 cut(s) 801
TspRI CASTG 2 cut(s) 244, 768
Tth111I GACNNNGTC 1 cut(s) 928
Van91I CCANNNNNTGG 3 cut(s) 368, 488, 509
VpaK11BI GGWCC 3 cut(s) 56, 347, 428
XapI RAATTY 3 cut(s) 141, 443, 960
XceI RCATGY 4 cut(s) 260, 337, 555, 668
XmiI GTMKAC 1 cut(s) 478
XspI CTAG 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.