RLG00000022565

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013911
Physical Location & Seq
Reverse (-)
3882900 .. 3884634
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022565

Sequence Viewer

Length: 1530 bp
ATGGCTAGTTCGGCCTCCAGTTCTACACTCAAGAAACTCGCTCTATTCCTCAACATATCCTTTCTCCTCTTATGTATCTGTCTCACAGCTACTAATTATCTCCATCCCAATAATCACAGCACAACCTTACCAAACCAGTCCACTATTCTTCGTGACACCATAGTTGATGATGGCTGCAGTAAACTCCATGAACAGCCAGATTTTGAAACCAAGTGTTTGTATGTCAAAACCCAAATAGGTTGCAGACCTGATGGTTACTTAAACTATCTCCAAATCTTTTATTGCAACTTTGGCCAGTTCCCAATAATAGCCCATGTTGCAATTCTACTTTGGCTTCTTGTTTTGTTCTACCTCTTGGGGAACACAGCTGCCAATTACTTCTGCTCCTCCTTAGAAAGCTTGTCCAAAATCTTGACGCTATCTCCCACCATTGCCGGAGTGACTCTCCTCTCGCTCGGAAATGGAGCCCCAGATGTCTTTTCGAGCATCGTTTCTTTCACGGGATCAGGTGATGGTGATGTCGGCCTAAACAGTGTGTTGGGGGGAGCCTTCTTTGTGTCTACTATTGTGGTAGTCTCATCCACACACATATACAACGTAAGGAAATTAGAAAGAGAAAGAGACCAATTATTAGTTCTCGGAGACACTACCACTAGTCCTTTATTGGGGCATGTCGTCGAAGAAAAAGTAGTTGTTTCTGTTCAAGATGATGATGGTAAAGATGGTGTCGATGATCAAGAACGGAAACTATATTCGTCTTTTGGTTATTTGGGGTATCTGTTGGAGCTGCCTCTGTCGTTGCCTAGAAGATTGACCATACCGGTTGTGAGCGAGGAAAATTGGTCCAAGCCATTTGCAGTTATTTCTGTGACTTTGGCACCAATGTCACTCGCTGCCCTTTTCAACACACAGGCTGAAAATGTGGGTTCCAGAACTAAATTAGTGGCTTACATAACAGCAGCTATTACTGGTTTGGTTTTTGGTAATATTGCTTATTTGAGCACGAAAAAGTCTGGTCCACCAAACAATAACAAGTACTTGTTCCCATGGCTTGTGGGAGGGTTTGTGATGAGTGTGGCCTGGACTTACCTCACTGCTGAAGAACTGGTGTGTTTGTTAGAGTCATATGGGAATATATTGGGGATTAGTCCTTCCATTCTGGGACTTACTATTCTGGCTTGGGGTAACTCTCTCGGAGATTTGATAGCTAATGTGGCCATGGCGGTTAACGGTGGGGCAGACGGAGCCCAGATTGCCATTACGGGCTGCTACGCCGGGCCGATGTTCAACACACTGGTGGGATTGGGGTTCTCACTGGTTTTGTCAGCTTGGAAGGAGTATCCATCCTCATACGTCATTGTTATCGACCATTCTCTGTACGAGACGGTGGCCTTTCTGATGGGGGGGTTGCTCTGGGCGCTTGCCTTGTTGCCAAACAAGAATATGAAGCTGGATCGTTATATGGGGAGTGGCCTAATCGCAATCTACTTGTGTTTTCTGTCTCTGAAGCTAGCCATGCTGCTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_Ca_ex

Protein Analysis

510

Amino Acids

55.04

Weight (kDa)

5.41

Isoelectric Point (pI)

30.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 109 - 197 5.8e-18 Sodium/calcium exchanger protein
Na_Ca_ex PF01699 351 - 503 2.8e-29 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013911)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17860 AT5G17860
fragaria_vesca FvH4_4g12810
malus_domestica MD12G1011500.v1.1
prunus_persica Prupe.7G014900_v2.0.a1 Prupe.7G014900_v2.0.a1
pyrus_communis pycom12g00900
rosa_chinensis RchiOBHm_Chr3g0496071
rosa_laevigata RLG00000022565
rosa_multiflora Rmu_sc0005781.1_g000006
rosa_roxburghii Rroxscaffold_6G00389590
rosa_rugosa Rorug03G0279300
rosa_samantha Rh3AG326400 Rh3BG362700 Rh3CG358800 Rh3DG362100
rosa_wichuraiana Rw3G028580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 877
AccI GTMKAC 1 cut(s) 560
AciI CCGC 1 cut(s) 1223
AclWI GGATC 2 cut(s) 511, 1461
AcoI YGGCCR 2 cut(s) 292, 1215
AcuI CTGAAG 2 cut(s) 1119, 1526
AfaI GTAC 2 cut(s) 1037, 1379
AfiI CCNNNNNNNGG 1 cut(s) 665
AgeI ACCGGT 1 cut(s) 820
AgsI TTSAA 4 cut(s) 206, 704, 904, 1288
AhlI ACTAGT 1 cut(s) 653
AjnI CCWGG 1 cut(s) 1079
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AleI CACNNNNGTG 1 cut(s) 1295
AluBI AGCT 9 cut(s) 89, 368, 399, 787, 962, 1208, 1328, 1450, 1510
AluI AGCT 9 cut(s) 89, 368, 399, 787, 962, 1208, 1328, 1450, 1510
Alw21I GWGCWC 1 cut(s) 1004
Alw26I GTCTC 6 cut(s) 86, 580, 615, 636, 1376, 1506
AlwI GGATC 2 cut(s) 511, 1461
AoxI GGCC 8 cut(s) 12, 292, 523, 1077, 1215, 1277, 1389, 1471
ApeKI GCWGC 8 cut(s) 174, 368, 787, 893, 959, 1266, 1519, 1522
AsiGI ACCGGT 1 cut(s) 820
AspLEI GCGC 1 cut(s) 1420
AspS9I GGNCC 3 cut(s) 843, 1016, 1277
AsuC2I CCSGG 1 cut(s) 1276
AsuHPI GGTGA 2 cut(s) 521, 527
AsuNHI GCTAGC 1 cut(s) 1510
AvaII GGWCC 2 cut(s) 843, 1016
BalI TGGCCA 2 cut(s) 294, 1217
BanI GGYRCC 1 cut(s) 877
BanII GRGCYC 2 cut(s) 469, 1249
Bbv12I GWGCWC 1 cut(s) 1004
BbvI GCAGC 8 cut(s) 161, 355, 774, 880, 971, 1253, 1506, 1509
BccI CCATC 8 cut(s) 111, 164, 245, 506, 707, 716, 1351, 1393
BciT130I CCWGG 1 cut(s) 1081
BciVI GTATCC 1 cut(s) 1350
BclI TGATCA 1 cut(s) 733
BcnI CCSGG 1 cut(s) 1276
BcoDI GTCTC 6 cut(s) 86, 580, 615, 636, 1376, 1506
BcuI ACTAGT 1 cut(s) 653
BfaI CTAG 4 cut(s) 6, 654, 804, 1511
BfmI CTRYAG 1 cut(s) 175
BfoI RGCGCY 1 cut(s) 1421
BfuI GTATCC 1 cut(s) 1350
BisI GCNGC 8 cut(s) 175, 369, 788, 894, 960, 1267, 1520, 1523
BlsI GCNGC 8 cut(s) 176, 370, 789, 895, 961, 1268, 1521, 1524
BmcAI AGTACT 1 cut(s) 1037
Bme1390I CCNGG 2 cut(s) 1081, 1276
Bme18I GGWCC 2 cut(s) 843, 1016
BmgT120I GGNCC 3 cut(s) 843, 1016, 1277
BmiI GGNNCC 5 cut(s) 466, 547, 879, 928, 1246
BmrFI CCNGG 2 cut(s) 1081, 1276
BmsI GCATC 1 cut(s) 495
BmtI GCTAGC 1 cut(s) 1514
BplI GAGNNNNNCTC 2 cut(s) 429, 461
BpuEI CTTGAG 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 1276
BsaI GGTCTC 1 cut(s) 615
BsaJI CCNNGG 2 cut(s) 1046, 1218
BsaWI WCCGGW 1 cut(s) 820
BsaXI ACNNNNNCTCC 6 cut(s) 368, 398, 406, 436, 1050, 1080
Bsc4I CCNNNNNNNGG 1 cut(s) 665
Bse118I RCCGGY 1 cut(s) 820
Bse1I ACTGG 7 cut(s) 18, 136, 295, 973, 1110, 1299, 1320
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 1 cut(s) 1081
BseDI CCNNGG 2 cut(s) 1046, 1218
BseGI GGATG 3 cut(s) 103, 578, 1343
BseLI CCNNNNNNNGG 1 cut(s) 665
BseMI GCAATG 1 cut(s) 429
BseNI ACTGG 7 cut(s) 18, 136, 295, 973, 1110, 1299, 1320
BseRI GAGGAG 3 cut(s) 56, 376, 437
BseXI GCAGC 8 cut(s) 161, 355, 774, 880, 971, 1253, 1506, 1509
BshFI GGCC 8 cut(s) 14, 294, 525, 1079, 1217, 1279, 1391, 1473
BshNI GGYRCC 1 cut(s) 877
BshTI ACCGGT 1 cut(s) 820
BsiHKAI GWGCWC 1 cut(s) 1004
BsiSI CCGG 3 cut(s) 435, 821, 1275
BslFI GGGAC 1 cut(s) 1176
BslI CCNNNNNNNGG 1 cut(s) 665
BsmAI GTCTC 6 cut(s) 86, 580, 615, 636, 1376, 1506
BsmBI CGTCTC 1 cut(s) 1376
BsmFI GGGAC 1 cut(s) 1176
BsnI GGCC 8 cut(s) 14, 294, 525, 1079, 1217, 1279, 1391, 1473
Bso31I GGTCTC 1 cut(s) 615
Bsp1286I GDGCHC 3 cut(s) 469, 1004, 1249
Bsp143I GATC 3 cut(s) 503, 733, 1453
Bsp19I CCATGG 2 cut(s) 1046, 1218
BspACI CCGC 1 cut(s) 1223
BspANI GGCC 8 cut(s) 14, 294, 525, 1079, 1217, 1279, 1391, 1473
BspLI GGNNCC 5 cut(s) 466, 547, 879, 928, 1246
BspMAI CTGCAG 1 cut(s) 179
BspOI GCTAGC 1 cut(s) 1514
BspPI GGATC 2 cut(s) 511, 1461
BspT107I GGYRCC 1 cut(s) 877
BspTNI GGTCTC 1 cut(s) 615
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 1 cut(s) 820
BsrI ACTGG 7 cut(s) 18, 136, 295, 973, 1110, 1299, 1320
BssAI RCCGGY 1 cut(s) 820
BssECI CCNNGG 2 cut(s) 1046, 1218
BssMI GATC 3 cut(s) 503, 733, 1453
BssT1I CCWWGG 2 cut(s) 1046, 1218
Bst2UI CCWGG 1 cut(s) 1081
Bst4CI ACNGT 3 cut(s) 533, 1232, 1387
BstC8I GCNNGC 2 cut(s) 1422, 1512
BstDEI CTNAG 1 cut(s) 391
BstDSI CCRYGG 2 cut(s) 1046, 1218
BstF5I GGATG 3 cut(s) 103, 578, 1343
BstH2I RGCGCY 1 cut(s) 1421
BstHHI GCGC 1 cut(s) 1420
BstKTI GATC 3 cut(s) 506, 736, 1456
BstMAI GTCTC 6 cut(s) 86, 580, 615, 636, 1376, 1506
BstMBI GATC 3 cut(s) 503, 733, 1453
BstMWI GCNNNNNNNGC 8 cut(s) 11, 291, 317, 1214, 1244, 1253, 1417, 1516
BstNI CCWGG 1 cut(s) 1081
BstNSI RCATGY 1 cut(s) 674
BstSCI CCNGG 2 cut(s) 1079, 1274
BstSFI CTRYAG 1 cut(s) 175
BstV1I GCAGC 8 cut(s) 161, 355, 774, 880, 971, 1253, 1506, 1509
BsuI GTATCC 1 cut(s) 1350
BsuRI GGCC 8 cut(s) 14, 294, 525, 1079, 1217, 1279, 1391, 1473
BtgI CCRYGG 2 cut(s) 1046, 1218
BtsCI GGATG 3 cut(s) 103, 578, 1343
BtsI GCAGTG 1 cut(s) 1092
BtsIMutI CAGTG 4 cut(s) 538, 1092, 1292, 1313
Cac8I GCNNGC 2 cut(s) 1422, 1512
CfoI GCGC 1 cut(s) 1420
Cfr10I RCCGGY 1 cut(s) 820
Cfr13I GGNCC 3 cut(s) 843, 1016, 1277
CseI GACGC 1 cut(s) 424
Csp6I GTAC 2 cut(s) 1036, 1378
CspAI ACCGGT 1 cut(s) 820
CviAII CATG 6 cut(s) 188, 314, 671, 1047, 1219, 1516
CviQI GTAC 2 cut(s) 1036, 1378
DdeI CTNAG 1 cut(s) 391
DpnI GATC 3 cut(s) 505, 735, 1455
DpnII GATC 3 cut(s) 503, 733, 1453
EaeI YGGCCR 2 cut(s) 292, 1215
Eco130I CCWWGG 2 cut(s) 1046, 1218
Eco24I GRGCYC 2 cut(s) 469, 1249
Eco31I GGTCTC 1 cut(s) 615
Eco47I GGWCC 2 cut(s) 843, 1016
Eco57I CTGAAG 2 cut(s) 1119, 1526
EcoRII CCWGG 1 cut(s) 1079
EcoT14I CCWWGG 2 cut(s) 1046, 1218
EcoT38I GRGCYC 2 cut(s) 469, 1249
ErhI CCWWGG 2 cut(s) 1046, 1218
Esp3I CGTCTC 1 cut(s) 1376
FaeI CATG 6 cut(s) 191, 317, 674, 1050, 1222, 1519
FaqI GGGAC 1 cut(s) 1176
FatI CATG 6 cut(s) 187, 313, 670, 1046, 1218, 1515
FauNDI CATATG 1 cut(s) 1126
FbaI TGATCA 1 cut(s) 733
FblI GTMKAC 1 cut(s) 560
Fnu4HI GCNGC 8 cut(s) 175, 369, 788, 894, 960, 1267, 1520, 1523
FokI GGATG 3 cut(s) 90, 565, 1330
FriOI GRGCYC 2 cut(s) 469, 1249
Fsp4HI GCNGC 8 cut(s) 175, 369, 788, 894, 960, 1267, 1520, 1523
FspBI CTAG 4 cut(s) 6, 654, 804, 1511
GlaI GCGC 1 cut(s) 1419
GluI GCNGC 8 cut(s) 175, 369, 788, 894, 960, 1267, 1520, 1523
HaeII RGCGCY 1 cut(s) 1421
HaeIII GGCC 8 cut(s) 14, 294, 525, 1079, 1217, 1279, 1391, 1473
HapII CCGG 3 cut(s) 435, 821, 1275
HgaI GACGC 1 cut(s) 424
HhaI GCGC 1 cut(s) 1420
Hin1II CATG 6 cut(s) 191, 317, 674, 1050, 1222, 1519
Hin6I GCGC 1 cut(s) 1418
HinP1I GCGC 1 cut(s) 1418
HincII GTYRAC 1 cut(s) 1228
HindII GTYRAC 1 cut(s) 1228
HindIII AAGCTT 1 cut(s) 397
HinfI GANTC 2 cut(s) 442, 1121
HpaI GTTAAC 1 cut(s) 1228
HpaII CCGG 3 cut(s) 435, 821, 1275
HphI GGTGA 2 cut(s) 521, 527
Hpy166II GTNNAC 5 cut(s) 141, 182, 561, 1019, 1228
Hpy188I TCNGA 6 cut(s) 458, 641, 1196, 1398, 1506, 1529
Hpy188III TCNNGA 6 cut(s) 31, 152, 412, 704, 737, 930
Hpy8I GTNNAC 5 cut(s) 141, 182, 561, 1019, 1228
Hpy99I CGWCG 1 cut(s) 680
HpyAV CCTTC 3 cut(s) 559, 1161, 1327
HpyCH4III ACNGT 3 cut(s) 533, 1232, 1387
HpyCH4IV ACGT 2 cut(s) 597, 1353
HpyCH4V TGCA 5 cut(s) 177, 243, 285, 320, 857
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 291, 317, 1214, 1244, 1253, 1417, 1516
HpyF3I CTNAG 1 cut(s) 391
HpySE526I ACGT 2 cut(s) 597, 1353
Hsp92II CATG 6 cut(s) 191, 317, 674, 1050, 1222, 1519
HspAI GCGC 1 cut(s) 1418
Ksp22I TGATCA 1 cut(s) 733
KspAI GTTAAC 1 cut(s) 1228
Kzo9I GATC 3 cut(s) 503, 733, 1453
LmnI GCTCC 5 cut(s) 389, 464, 545, 784, 1244
Lsp1109I GCAGC 8 cut(s) 161, 355, 774, 880, 971, 1253, 1506, 1509
LweI GCATC 1 cut(s) 495
MaeI CTAG 4 cut(s) 6, 654, 804, 1511
MaeII ACGT 2 cut(s) 597, 1353
MaeIII GTNAC 6 cut(s) 152, 254, 439, 868, 885, 1184
MalI GATC 3 cut(s) 505, 735, 1455
MboI GATC 3 cut(s) 503, 733, 1453
MboII GAAGA 4 cut(s) 140, 692, 819, 1112
MhlI GDGCHC 3 cut(s) 469, 1004, 1249
MlsI TGGCCA 2 cut(s) 294, 1217
MluCI AATT 7 cut(s) 94, 321, 373, 605, 626, 838, 938
MluNI TGGCCA 2 cut(s) 294, 1217
MlyI GAGTC 2 cut(s) 436, 1130
MmeI TCCRAC 1 cut(s) 762
Mox20I TGGCCA 2 cut(s) 294, 1217
MscI TGGCCA 2 cut(s) 294, 1217
MseI TTAA 2 cut(s) 260, 1227
MslI CAYNNNNRTG 1 cut(s) 1295
Msp20I TGGCCA 2 cut(s) 294, 1217
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 3 cut(s) 435, 821, 1275
MspR9I CCNGG 2 cut(s) 1081, 1276
MvaI CCWGG 1 cut(s) 1081
MwoI GCNNNNNNNGC 8 cut(s) 11, 291, 317, 1214, 1244, 1253, 1417, 1516
NciI CCSGG 1 cut(s) 1276
NcoI CCATGG 2 cut(s) 1046, 1218
NdeI CATATG 1 cut(s) 1126
NdeII GATC 3 cut(s) 503, 733, 1453
NheI GCTAGC 1 cut(s) 1510
NlaIII CATG 6 cut(s) 191, 317, 674, 1050, 1222, 1519
NlaIV GGNNCC 5 cut(s) 466, 547, 879, 928, 1246
NmuCI GTSAC 4 cut(s) 152, 439, 868, 885
NspI RCATGY 1 cut(s) 674
OliI CACNNNNGTG 1 cut(s) 1295
PinAI ACCGGT 1 cut(s) 820
PkrI GCNGC 8 cut(s) 176, 370, 789, 895, 961, 1268, 1521, 1524
PleI GAGTC 2 cut(s) 436, 1129
PpsI GAGTC 2 cut(s) 436, 1129
Psp6I CCWGG 1 cut(s) 1079
PspGI CCWGG 1 cut(s) 1079
PspN4I GGNNCC 5 cut(s) 466, 547, 879, 928, 1246
PspPI GGNCC 3 cut(s) 843, 1016, 1277
PstI CTGCAG 1 cut(s) 179
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 2 cut(s) 1037, 1379
RsaNI GTAC 2 cut(s) 1036, 1378
RseI CAYNNNNRTG 1 cut(s) 1295
SaqAI TTAA 2 cut(s) 260, 1227
SatI GCNGC 8 cut(s) 175, 369, 788, 894, 960, 1267, 1520, 1523
Sau3AI GATC 3 cut(s) 503, 733, 1453
Sau96I GGNCC 3 cut(s) 843, 1016, 1277
ScaI AGTACT 1 cut(s) 1037
SchI GAGTC 2 cut(s) 436, 1130
ScrFI CCNGG 2 cut(s) 1081, 1276
SduI GDGCHC 3 cut(s) 469, 1004, 1249
SfaNI GCATC 1 cut(s) 495
SfcI CTRYAG 1 cut(s) 175
SinI GGWCC 2 cut(s) 843, 1016
SmiMI CAYNNNNRTG 1 cut(s) 1295
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SpeI ACTAGT 1 cut(s) 653
Sse9I AATT 7 cut(s) 94, 321, 373, 605, 626, 838, 938
SsiI CCGC 1 cut(s) 1223
SspI AATATT 1 cut(s) 988
SspMI CTAG 4 cut(s) 6, 654, 804, 1511
StyD4I CCNGG 2 cut(s) 1079, 1274
StyI CCWWGG 2 cut(s) 1046, 1218
TaaI ACNGT 3 cut(s) 533, 1232, 1387
TaiI ACGT 2 cut(s) 600, 1356
TaqI TCGA 4 cut(s) 482, 678, 729, 1365
TasI AATT 7 cut(s) 94, 321, 373, 605, 626, 838, 938
TatI WGTACW 1 cut(s) 1035
Tru1I TTAA 2 cut(s) 260, 1227
Tru9I TTAA 2 cut(s) 260, 1227
TscAI CASTG 4 cut(s) 538, 1099, 1299, 1320
TseFI GTSAC 4 cut(s) 152, 439, 868, 885
TseI GCWGC 8 cut(s) 174, 368, 787, 893, 959, 1266, 1519, 1522
Tsp45I GTSAC 4 cut(s) 152, 439, 868, 885
TspDTI ATGAA 2 cut(s) 204, 1460
TspGWI ACGGA 2 cut(s) 757, 1257
TspRI CASTG 4 cut(s) 538, 1099, 1299, 1320
VpaK11BI GGWCC 2 cut(s) 843, 1016
XceI RCATGY 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 560
XspI CTAG 4 cut(s) 6, 654, 804, 1511
ZrmI AGTACT 1 cut(s) 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.