pycom12g00900

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013911
Physical Location & Seq
Forward (+)
762798 .. 763442
645 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00900.1

Sequence Viewer

Length: 645 bp
ATGTTACTGGCAGCCCTTTGTAACACACAGAGGGAAGATTTGAGTTCAAGAGGTAAGTTGGTAACTTACATGACAGCAGTGCTGATTGGGTTAATTTTGAGTAACCTTGCATACGTGACTACAAATAAGTGTAGCCCACCAAAGGAGTGCTTGTTCCCTTGGCTTGTTGGTGGGTTTTTGATGAGTGTAGCTTGGACATATATTACTGCTGAAGAGTTGGTTGGCTTGTTAGAATCATATGGTAATATTTTAGGGATAAGCCCTTCAATTCTAGGACTTACTGTCCTAGCTTGGGGTAACTCCCTCAATGATTTGATAGCTAATGCTACAATGGCAGTGAAGGGTGGGACAGATGGGGCCCAAATTGCTATTTCAGGATGCTATGCAGGGCCCATGTTTAACACATTGGTGGGAGTGGGAACGTCACTGGTTCTGTCATCATGGTTTGAGTATCCATCTTCATATGTCATACCTACCGACCATTCCCTCTACGAAACAATAGGGTTTTTGATGGGTGGTTTACTTTGGGCACTTGTGATCTTGCCAAAGAAGGGTATGAAGCTGGATAGATACATGGGGAGTGGCCTTGTGGCAATTTACTTGTGTTTTCTGTTTGTGAGGTTAGCCAGGCTCTTGTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_Ca_ex

Protein Analysis

215

Amino Acids

23.14

Weight (kDa)

6.55

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 55 - 208 1.5e-30 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013911)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17860 AT5G17860
fragaria_vesca FvH4_4g12810
malus_domestica MD12G1011500.v1.1
prunus_persica Prupe.7G014900_v2.0.a1 Prupe.7G014900_v2.0.a1
pyrus_communis pycom12g00900
rosa_chinensis RchiOBHm_Chr3g0496071
rosa_laevigata RLG00000022565
rosa_multiflora Rmu_sc0005781.1_g000006
rosa_roxburghii Rroxscaffold_6G00389590
rosa_rugosa Rorug03G0279300
rosa_samantha Rh3AG326400 Rh3BG362700 Rh3CG358800 Rh3DG362100
rosa_wichuraiana Rw3G028580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 231
AfiI CCNNNNNNNGG 3 cut(s) 142, 292, 551
AgsI TTSAA 2 cut(s) 48, 267
AhdI GACNNNNNGTC 1 cut(s) 281
AjnI CCWGG 1 cut(s) 626
AjuI GAANNNNNNNTTGG 2 cut(s) 204, 236
AleI CACNNNNGTG 1 cut(s) 407
AloI GAACNNNNNNTCC 2 cut(s) 137, 169
AluBI AGCT 4 cut(s) 191, 290, 320, 562
AluI AGCT 4 cut(s) 191, 290, 320, 562
AoxI GGCC 3 cut(s) 357, 389, 583
ApaI GGGCCC 2 cut(s) 361, 393
ApeKI GCWGC 1 cut(s) 11
AspS9I GGNCC 4 cut(s) 357, 358, 389, 390
BaeGI GKGCMC 3 cut(s) 361, 393, 532
BanII GRGCYC 2 cut(s) 361, 393
BbvI GCAGC 1 cut(s) 23
BccI CCATC 3 cut(s) 347, 463, 505
BciT130I CCWGG 1 cut(s) 628
BciVI GTATCC 1 cut(s) 462
BfaI CTAG 2 cut(s) 272, 287
BfuI GTATCC 1 cut(s) 462
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 628
BmeRI GACNNNNNGTC 1 cut(s) 281
BmgT120I GGNCC 4 cut(s) 357, 358, 389, 390
BmiI GGNNCC 3 cut(s) 358, 359, 391
BmrFI CCNGG 1 cut(s) 628
BmsI GCATC 1 cut(s) 368
BsaAI YACGTR 1 cut(s) 115
BsaJI CCNNGG 1 cut(s) 158
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 292, 551
Bse1I ACTGG 2 cut(s) 12, 432
BseBI CCWGG 1 cut(s) 628
BseDI CCNNGG 1 cut(s) 158
BseGI GGATG 1 cut(s) 383
BseLI CCNNNNNNNGG 3 cut(s) 142, 292, 551
BseNI ACTGG 2 cut(s) 12, 432
BseSI GKGCMC 3 cut(s) 361, 393, 532
BseXI GCAGC 1 cut(s) 23
BshFI GGCC 3 cut(s) 359, 391, 585
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 3 cut(s) 142, 292, 551
BsmFI GGGAC 1 cut(s) 361
BsnI GGCC 3 cut(s) 359, 391, 585
Bsp120I GGGCCC 2 cut(s) 357, 389
Bsp1286I GDGCHC 3 cut(s) 361, 393, 532
Bsp143I GATC 1 cut(s) 537
BspANI GGCC 3 cut(s) 359, 391, 585
BspLI GGNNCC 3 cut(s) 358, 359, 391
BsrI ACTGG 2 cut(s) 12, 432
BssECI CCNNGG 1 cut(s) 158
BssMI GATC 1 cut(s) 537
BssT1I CCWWGG 1 cut(s) 158
Bst2UI CCWGG 1 cut(s) 628
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 1 cut(s) 207
BstBAI YACGTR 1 cut(s) 115
BstF5I GGATG 1 cut(s) 383
BstKTI GATC 1 cut(s) 540
BstMBI GATC 1 cut(s) 537
BstMWI GCNNNNNNNGC 2 cut(s) 332, 365
BstNI CCWGG 1 cut(s) 628
BstSCI CCNGG 1 cut(s) 626
BstSLI GKGCMC 3 cut(s) 361, 393, 532
BstV1I GCAGC 1 cut(s) 23
BsuI GTATCC 1 cut(s) 462
BsuRI GGCC 3 cut(s) 359, 391, 585
BtsCI GGATG 1 cut(s) 383
BtsI GCAGTG 2 cut(s) 84, 342
BtsIMutI CAGTG 3 cut(s) 84, 342, 425
Cfr13I GGNCC 4 cut(s) 357, 358, 389, 390
CviAII CATG 4 cut(s) 70, 394, 441, 574
DpnI GATC 1 cut(s) 539
DpnII GATC 1 cut(s) 537
DriI GACNNNNNGTC 1 cut(s) 281
Eam1104I CTCTTC 1 cut(s) 207
Eam1105I GACNNNNNGTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 158
Eco24I GRGCYC 2 cut(s) 361, 393
Eco57I CTGAAG 1 cut(s) 231
EcoO109I RGGNCCY 2 cut(s) 357, 389
EcoRII CCWGG 1 cut(s) 626
EcoT14I CCWWGG 1 cut(s) 158
EcoT38I GRGCYC 2 cut(s) 361, 393
ErhI CCWWGG 1 cut(s) 158
FaeI CATG 4 cut(s) 73, 397, 444, 577
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 1 cut(s) 361
FatI CATG 4 cut(s) 69, 393, 440, 573
FauNDI CATATG 2 cut(s) 238, 463
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 1 cut(s) 390
FriOI GRGCYC 2 cut(s) 361, 393
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 2 cut(s) 272, 287
GluI GCNGC 1 cut(s) 12
HaeIII GGCC 3 cut(s) 359, 391, 585
Hin1II CATG 4 cut(s) 73, 397, 444, 577
HinfI GANTC 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 521
Hpy188III TCNNGA 2 cut(s) 48, 375
Hpy8I GTNNAC 1 cut(s) 521
HpyAV CCTTC 3 cut(s) 273, 334, 544
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 2 cut(s) 114, 422
HpyCH4V TGCA 2 cut(s) 110, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 332, 365
HpySE526I ACGT 2 cut(s) 114, 422
Hsp92II CATG 4 cut(s) 73, 397, 444, 577
Kzo9I GATC 1 cut(s) 537
LpnPI CCDG 6 cut(s) 360, 372, 413, 548, 613, 640
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 1 cut(s) 368
MaeI CTAG 2 cut(s) 272, 287
MaeII ACGT 2 cut(s) 114, 422
MaeIII GTNAC 7 cut(s) 3, 20, 61, 101, 115, 296, 423
MalI GATC 1 cut(s) 539
MboI GATC 1 cut(s) 537
MboII GAAGA 3 cut(s) 47, 224, 450
MhlI GDGCHC 3 cut(s) 361, 393, 532
MluCI AATT 4 cut(s) 93, 267, 363, 594
MnlI CCTC 5 cut(s) 24, 44, 314, 497, 612
MseI TTAA 2 cut(s) 92, 399
MslI CAYNNNNRTG 1 cut(s) 407
MspR9I CCNGG 1 cut(s) 628
MvaI CCWGG 1 cut(s) 628
MwoI GCNNNNNNNGC 2 cut(s) 332, 365
NdeI CATATG 2 cut(s) 238, 463
NdeII GATC 1 cut(s) 537
NlaIII CATG 4 cut(s) 73, 397, 444, 577
NlaIV GGNNCC 3 cut(s) 358, 359, 391
NmuCI GTSAC 2 cut(s) 115, 423
OliI CACNNNNGTG 1 cut(s) 407
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 1 cut(s) 13
Ppu21I YACGTR 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 626
PspGI CCWGG 1 cut(s) 626
PspN4I GGNNCC 3 cut(s) 358, 359, 391
PspOMI GGGCCC 2 cut(s) 357, 389
PspPI GGNCC 4 cut(s) 357, 358, 389, 390
RseI CAYNNNNRTG 1 cut(s) 407
SaqAI TTAA 2 cut(s) 92, 399
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 1 cut(s) 537
Sau96I GGNCC 4 cut(s) 357, 358, 389, 390
ScrFI CCNGG 1 cut(s) 628
SduI GDGCHC 3 cut(s) 361, 393, 532
SfaNI GCATC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 407
Sse9I AATT 4 cut(s) 93, 267, 363, 594
SspI AATATT 1 cut(s) 247
SspMI CTAG 2 cut(s) 272, 287
StyD4I CCNGG 1 cut(s) 626
StyI CCWWGG 1 cut(s) 158
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 2 cut(s) 117, 425
TasI AATT 4 cut(s) 93, 267, 363, 594
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 2 cut(s) 92, 399
Tru9I TTAA 2 cut(s) 92, 399
TscAI CASTG 3 cut(s) 84, 342, 432
TseFI GTSAC 2 cut(s) 115, 423
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 2 cut(s) 115, 423
TspDTI ATGAA 2 cut(s) 450, 572
TspRI CASTG 3 cut(s) 84, 342, 432
XspI CTAG 2 cut(s) 272, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.