MD12G1015700.v1.1

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008046
Physical Location & Seq
Forward (+)
1575101 .. 1580525
5425 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1015700.v1.1.491

Sequence Viewer

Length: 1179 bp
ATGGGAATGGGATTGCTGGGGAGCTCAACAATCCCATTGGGAGTGGGAATGGGAGGAGTAGTGGGCGAGCATTGCTGGGAATGGGGGAGAAAGTACTTGAAGTACGAACTCTGCAGCGTGAAGGACGGCATGTCGTTGGCGCTGGGTGTAATCAGCGTACTCAGTTGGGGTGTCGCTGAGGTTCCCCAAATCATCACCAACTACAAGGCCAAATCCAGTCAAGGCCTCTCTCTTGCTTTCTTGATCACTTGGATTATCGGGGACCTGTTTAACCTTTTTGGCTGCCTGTTAGAACCAGCTACTCTTCCAACACAGTACTATACAGCAATTTTGTATTTAACAACCACATTGGTTCTTTCTGTACAGACCATATACTATGGTTATGTATATCCTCGTCTTAATAATTTGAGACAGAAAAAGGGTTCCAAGACTAATCCGGCTGAGGTTCCAGGAAAAGCTAGTGGCAGGGTTAATGTGATTGACACCCCCTCTGCAGGAAATGAATGGACTTCACCCATTCTTTGTCCCAATATTTCTCCTGGACGAGAATCATACTACATATCGGCAAGATCTTTATCAAGGAGTCACACCCCTTCAGAAGGATTTCATGCAGCACAAAGAAAAATTCTTCCATTTAATCGTAATCGAAATCCAAATGAAGAGCCCTTGCTTGGTGCACTTGCATCTACACAGTCTGCACCATCTAATAATGTCAAGACTACGCTGTGTGTGGTCTCGCTGATGACCCTCTTTTGCACTTTTAATCAACGATCAGTTGATTTAATTGTTGACAATCCAAATCGAGGAGTAGTGATGCAAGTAGGAAGAAGGCTGTTGCAGGTAAATAGTGGGCTGTTGCGAGAAATGGGTACTAAACATAGCAGTGGGATAGGAAGTTTCCTTGGTTGGGGAATGGCAGTTATTTACATTGGTGGACGGTTTCCCCAAATTTACTTAAATATCCGCAAGGGGAATGTTGACGGGCTTAATCCTTTTATGTTCATCTTTGCTGTTGTTGGAAATGCAGCTTACGTCGCAAGCATTTTGGTGAACAGCATGGAGTGGTCGAAAATCAGTCCGAATCTTGCGTGGCTGGTGGATGCTGGAGGATGCATGCTTCTGGATATTTTTTTTAGATACTACTTCAATTCTTCCAATACTGGAGACACCGAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

393

Amino Acids

42.92

Weight (kDa)

9.15

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQ-loop PF04193 45 - 100 9.8e-18 PQ loop repeat
PQ-loop PF04193 297 - 355 4.5e-15 PQ loop repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 829
AciI CCGC 1 cut(s) 964
AcsI RAATTY 2 cut(s) 624, 948
AcuI CTGAAG 1 cut(s) 579
AfaI GTAC 6 cut(s) 95, 104, 159, 317, 363, 871
AfiI CCNNNNNNNGG 5 cut(s) 494, 599, 671, 803, 907
AgsI TTSAA 2 cut(s) 100, 1147
AhdI GACNNNNNGTC 1 cut(s) 130
AjnI CCWGG 2 cut(s) 448, 538
AluBI AGCT 4 cut(s) 24, 299, 458, 1028
AluI AGCT 4 cut(s) 24, 299, 458, 1028
Alw21I GWGCWC 2 cut(s) 26, 679
Alw26I GTCTC 3 cut(s) 403, 739, 1158
Alw44I GTGCAC 1 cut(s) 675
AoxI GGCC 2 cut(s) 207, 223
ApaLI GTGCAC 1 cut(s) 675
ApeKI GCWGC 4 cut(s) 114, 282, 611, 1025
ApoI RAATTY 2 cut(s) 624, 948
Asp700I GAANNNNTTC 1 cut(s) 603
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 1 cut(s) 262
AsuHPI GGTGA 3 cut(s) 187, 504, 1060
AvaII GGWCC 1 cut(s) 262
BaeGI GKGCMC 1 cut(s) 679
BanII GRGCYC 2 cut(s) 26, 666
Bbv12I GWGCWC 2 cut(s) 26, 679
BbvCI CCTCAGC 2 cut(s) 177, 441
BbvI GCAGC 4 cut(s) 126, 269, 623, 1037
BccI CCATC 1 cut(s) 709
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 2 cut(s) 450, 540
BclI TGATCA 1 cut(s) 243
BcoDI GTCTC 3 cut(s) 403, 739, 1158
BfaI CTAG 1 cut(s) 459
BfmI CTRYAG 2 cut(s) 112, 492
BfoI RGCGCY 1 cut(s) 143
BfuAI ACCTGC 1 cut(s) 829
BglII AGATCT 1 cut(s) 569
BisI GCNGC 4 cut(s) 115, 283, 612, 1026
BlsI GCNGC 4 cut(s) 116, 284, 613, 1027
BmcAI AGTACT 2 cut(s) 95, 317
Bme1390I CCNGG 2 cut(s) 450, 540
Bme18I GGWCC 1 cut(s) 262
BmeRI GACNNNNNGTC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 4 cut(s) 183, 263, 424, 447
BmrFI CCNGG 2 cut(s) 450, 540
BmsI GCATC 4 cut(s) 692, 804, 1090, 1100
BpmI CTGGAG 1 cut(s) 1125
Bpu10I CCTNAGC 2 cut(s) 177, 441
BsaBI GATNNNNATC 1 cut(s) 574
BsaI GGTCTC 1 cut(s) 739
BsaJI CCNNGG 1 cut(s) 901
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 5 cut(s) 494, 599, 671, 803, 907
Bse1I ACTGG 2 cut(s) 216, 1165
Bse3DI GCAATG 1 cut(s) 70
Bse8I GATNNNNATC 1 cut(s) 574
BseBI CCWGG 2 cut(s) 450, 540
BseDI CCNNGG 1 cut(s) 901
BseGI GGATG 2 cut(s) 1105, 1115
BseJI GATNNNNATC 1 cut(s) 574
BseLI CCNNNNNNNGG 5 cut(s) 494, 599, 671, 803, 907
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 3 cut(s) 168, 175, 432
BseNI ACTGG 2 cut(s) 216, 1165
BseRI GAGGAG 2 cut(s) 69, 819
BseSI GKGCMC 1 cut(s) 679
BseXI GCAGC 4 cut(s) 126, 269, 623, 1037
BseYI CCCAGC 3 cut(s) 16, 75, 142
BsgI GTGCAG 1 cut(s) 681
BshFI GGCC 2 cut(s) 209, 225
BsiHKAI GWGCWC 2 cut(s) 26, 679
BsiSI CCGG 1 cut(s) 437
BslFI GGGAC 2 cut(s) 275, 510
BslI CCNNNNNNNGG 5 cut(s) 494, 599, 671, 803, 907
BsmAI GTCTC 3 cut(s) 403, 739, 1158
BsmFI GGGAC 2 cut(s) 275, 510
BsnI GGCC 2 cut(s) 209, 225
Bso31I GGTCTC 1 cut(s) 739
Bsp1286I GDGCHC 3 cut(s) 26, 666, 679
Bsp1407I TGTACA 1 cut(s) 361
Bsp143I GATC 3 cut(s) 243, 569, 770
BspACI CCGC 1 cut(s) 964
BspANI GGCC 2 cut(s) 209, 225
BspCNI CTCAG 3 cut(s) 169, 174, 433
BspLI GGNNCC 4 cut(s) 183, 263, 424, 447
BspMAI CTGCAG 2 cut(s) 116, 496
BspMI ACCTGC 1 cut(s) 829
BspQI GCTCTTC 1 cut(s) 654
BspTNI GGTCTC 1 cut(s) 739
BsrDI GCAATG 1 cut(s) 70
BsrGI TGTACA 1 cut(s) 361
BsrI ACTGG 2 cut(s) 216, 1165
BssECI CCNNGG 1 cut(s) 901
BssMI GATC 3 cut(s) 243, 569, 770
BssT1I CCWWGG 1 cut(s) 901
Bst2UI CCWGG 2 cut(s) 450, 540
Bst4CI ACNGT 3 cut(s) 315, 693, 939
Bst6I CTCTTC 2 cut(s) 309, 654
BstAUI TGTACA 1 cut(s) 361
BstC8I GCNNGC 3 cut(s) 68, 1039, 1115
BstDEI CTNAG 3 cut(s) 161, 177, 441
BstENI CCTNNNNNAGG 1 cut(s) 597
BstF5I GGATG 2 cut(s) 1105, 1115
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 3 cut(s) 246, 572, 773
BstMAI GTCTC 3 cut(s) 403, 739, 1158
BstMBI GATC 3 cut(s) 243, 569, 770
BstMWI GCNNNNNNNGC 2 cut(s) 72, 1034
BstNI CCWGG 2 cut(s) 450, 540
BstNSI RCATGY 2 cut(s) 133, 1117
BstSCI CCNGG 2 cut(s) 448, 538
BstSFI CTRYAG 2 cut(s) 112, 492
BstSLI GKGCMC 1 cut(s) 679
BstV1I GCAGC 4 cut(s) 126, 269, 623, 1037
BstX2I RGATCY 1 cut(s) 569
BstYI RGATCY 1 cut(s) 569
BsuRI GGCC 2 cut(s) 209, 225
BtsCI GGATG 2 cut(s) 1105, 1115
BtsI GCAGTG 1 cut(s) 889
BtsIMutI CAGTG 1 cut(s) 889
BveI ACCTGC 1 cut(s) 829
Cac8I GCNNGC 3 cut(s) 68, 1039, 1115
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 262
Csp6I GTAC 6 cut(s) 94, 103, 158, 316, 362, 870
CviAII CATG 4 cut(s) 130, 608, 1057, 1114
CviQI GTAC 6 cut(s) 94, 103, 158, 316, 362, 870
DdeI CTNAG 3 cut(s) 161, 177, 441
DpnI GATC 3 cut(s) 245, 571, 772
DpnII GATC 3 cut(s) 243, 569, 770
DriI GACNNNNNGTC 1 cut(s) 130
Eam1104I CTCTTC 2 cut(s) 309, 654
Eam1105I GACNNNNNGTC 1 cut(s) 130
EarI CTCTTC 2 cut(s) 309, 654
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 901
Eco147I AGGCCT 1 cut(s) 225
Eco24I GRGCYC 2 cut(s) 26, 666
Eco31I GGTCTC 1 cut(s) 739
Eco47I GGWCC 1 cut(s) 262
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 579
EcoICRI GAGCTC 1 cut(s) 24
EcoNI CCTNNNNNAGG 1 cut(s) 597
EcoO109I RGGNCCY 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 448, 538
EcoT14I CCWWGG 1 cut(s) 901
EcoT22I ATGCAT 1 cut(s) 1115
EcoT38I GRGCYC 2 cut(s) 26, 666
ErhI CCWWGG 1 cut(s) 901
FaeI CATG 4 cut(s) 133, 611, 1060, 1117
FaqI GGGAC 2 cut(s) 275, 510
FatI CATG 4 cut(s) 129, 607, 1056, 1113
FbaI TGATCA 1 cut(s) 243
Fnu4HI GCNGC 4 cut(s) 115, 283, 612, 1026
FokI GGATG 2 cut(s) 1112, 1122
FriOI GRGCYC 2 cut(s) 26, 666
Fsp4HI GCNGC 4 cut(s) 115, 283, 612, 1026
FspBI CTAG 1 cut(s) 459
GlaI GCGC 1 cut(s) 141
GluI GCNGC 4 cut(s) 115, 283, 612, 1026
GsaI CCCAGC 3 cut(s) 20, 79, 146
GsuI CTGGAG 1 cut(s) 1125
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 2 cut(s) 209, 225
HapII CCGG 1 cut(s) 437
HhaI GCGC 1 cut(s) 142
Hin1II CATG 4 cut(s) 133, 611, 1060, 1117
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HincII GTYRAC 2 cut(s) 790, 979
HindII GTYRAC 2 cut(s) 790, 979
HinfI GANTC 3 cut(s) 548, 583, 1081
HpaII CCGG 1 cut(s) 437
HphI GGTGA 3 cut(s) 187, 504, 1060
Hpy166II GTNNAC 5 cut(s) 677, 790, 935, 979, 1051
Hpy188I TCNGA 2 cut(s) 598, 1080
Hpy188III TCNNGA 3 cut(s) 241, 715, 1121
Hpy8I GTNNAC 5 cut(s) 677, 790, 935, 979, 1051
Hpy99I CGWCG 1 cut(s) 1037
HpyAV CCTTC 4 cut(s) 115, 593, 603, 822
HpyCH4III ACNGT 3 cut(s) 315, 693, 939
HpyCH4IV ACGT 1 cut(s) 1032
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 1034
HpyF3I CTNAG 3 cut(s) 161, 177, 441
HpySE526I ACGT 1 cut(s) 1032
Hsp92II CATG 4 cut(s) 133, 611, 1060, 1117
HspAI GCGC 1 cut(s) 140
Ksp22I TGATCA 1 cut(s) 243
Kzo9I GATC 3 cut(s) 243, 569, 770
LguI GCTCTTC 1 cut(s) 654
LmnI GCTCC 1 cut(s) 21
Lsp1109I GCAGC 4 cut(s) 126, 269, 623, 1037
LweI GCATC 4 cut(s) 692, 804, 1090, 1100
MaeI CTAG 1 cut(s) 459
MaeII ACGT 1 cut(s) 1032
MaeIII GTNAC 1 cut(s) 584
MalI GATC 3 cut(s) 245, 571, 772
MboI GATC 3 cut(s) 243, 569, 770
MboII GAAGA 5 cut(s) 296, 620, 671, 837, 1143
MflI RGATCY 1 cut(s) 569
MhlI GDGCHC 3 cut(s) 26, 666, 679
MluCI AATT 6 cut(s) 327, 403, 624, 783, 948, 1147
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 2 cut(s) 332, 997
MnlI CCTC 9 cut(s) 47, 172, 236, 402, 436, 499, 758, 797, 1100
Mph1103I ATGCAT 1 cut(s) 1115
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 9 cut(s) 270, 338, 399, 471, 636, 762, 782, 956, 987
MslI CAYNNNNRTG 3 cut(s) 882, 1046, 1172
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 450, 540
MvaI CCWGG 2 cut(s) 450, 540
MwoI GCNNNNNNNGC 2 cut(s) 72, 1034
NdeII GATC 3 cut(s) 243, 569, 770
NlaIII CATG 4 cut(s) 133, 611, 1060, 1117
NlaIV GGNNCC 4 cut(s) 183, 263, 424, 447
NmuCI GTSAC 1 cut(s) 584
NsiI ATGCAT 1 cut(s) 1115
NspI RCATGY 2 cut(s) 133, 1117
PaeI GCATGC 1 cut(s) 1117
PceI AGGCCT 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 654
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 2 cut(s) 548, 1081
PfoI TCCNGGA 2 cut(s) 448, 538
PkrI GCNGC 4 cut(s) 116, 284, 613, 1027
PleI GAGTC 1 cut(s) 591
PpsI GAGTC 1 cut(s) 591
PpuMI RGGWCCY 1 cut(s) 262
Psp124BI GAGCTC 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 2 cut(s) 448, 538
PspFI CCCAGC 3 cut(s) 16, 75, 142
PspGI CCWGG 2 cut(s) 448, 538
PspN4I GGNNCC 4 cut(s) 183, 263, 424, 447
PspPI GGNCC 1 cut(s) 262
PspPPI RGGWCCY 1 cut(s) 262
PstI CTGCAG 2 cut(s) 116, 496
PsuI RGATCY 1 cut(s) 569
RsaI GTAC 6 cut(s) 95, 104, 159, 317, 363, 871
RsaNI GTAC 6 cut(s) 94, 103, 158, 316, 362, 870
RseI CAYNNNNRTG 3 cut(s) 882, 1046, 1172
SacI GAGCTC 1 cut(s) 26
SapI GCTCTTC 1 cut(s) 654
SaqAI TTAA 9 cut(s) 270, 338, 399, 471, 636, 762, 782, 956, 987
SatI GCNGC 4 cut(s) 115, 283, 612, 1026
Sau3AI GATC 3 cut(s) 243, 569, 770
Sau96I GGNCC 1 cut(s) 262
ScaI AGTACT 2 cut(s) 95, 317
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 2 cut(s) 450, 540
SduI GDGCHC 3 cut(s) 26, 666, 679
SfaNI GCATC 4 cut(s) 692, 804, 1090, 1100
SfcI CTRYAG 2 cut(s) 112, 492
SinI GGWCC 1 cut(s) 262
SmiMI CAYNNNNRTG 3 cut(s) 882, 1046, 1172
SphI GCATGC 1 cut(s) 1117
Sse9I AATT 6 cut(s) 327, 403, 624, 783, 948, 1147
SseBI AGGCCT 1 cut(s) 225
SsiI CCGC 1 cut(s) 964
SspI AATATT 1 cut(s) 532
SspMI CTAG 1 cut(s) 459
SstI GAGCTC 1 cut(s) 26
StuI AGGCCT 1 cut(s) 225
StyD4I CCNGG 2 cut(s) 448, 538
StyI CCWWGG 1 cut(s) 901
TaaI ACNGT 3 cut(s) 315, 693, 939
TaiI ACGT 1 cut(s) 1035
TaqI TCGA 3 cut(s) 646, 802, 1067
TasI AATT 6 cut(s) 327, 403, 624, 783, 948, 1147
TatI WGTACW 3 cut(s) 93, 315, 361
TfiI GAWTC 2 cut(s) 548, 1081
Tru1I TTAA 9 cut(s) 270, 338, 399, 471, 636, 762, 782, 956, 987
Tru9I TTAA 9 cut(s) 270, 338, 399, 471, 636, 762, 782, 956, 987
TscAI CASTG 1 cut(s) 889
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 4 cut(s) 114, 282, 611, 1025
Tsp45I GTSAC 1 cut(s) 584
TspDTI ATGAA 4 cut(s) 516, 596, 672, 991
TspRI CASTG 1 cut(s) 889
VneI GTGCAC 1 cut(s) 675
VpaK11BI GGWCC 1 cut(s) 262
XagI CCTNNNNNAGG 1 cut(s) 597
XapI RAATTY 2 cut(s) 624, 948
XceI RCATGY 2 cut(s) 133, 1117
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 1 cut(s) 459
ZrmI AGTACT 2 cut(s) 95, 317
Zsp2I ATGCAT 1 cut(s) 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.