Rorug03G0271300

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000072
Physical Location & Seq
Reverse (-)
26766045 .. 26769972
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0271300.1

Sequence Viewer

Length: 3540 bp
ATGGCCTCGAGCTCCCAAACAACCTCTTCATCAGCTCCTCAGTGGAAGCATGATGTGTTTCTCAGCTTCAGGGGTGAAGACACCCGGAAGGGTATTGTATCCTCTTTATACCATGAATTGAAAACCAGAGGAATACAAACATTCATGGATGACCAAGGGCTTCAGCAAGGGATGCCTATTTCTCCAAGTCTCCTAACAGCAATTGAGGAATCCAGGTGTGCAGTCGTTCTTCTATCGCCAAACTATGCTTCTTCTACATGGTGTTTGGATGAACTTGCAAAGATTCTTCAATGCTCTGAAGCCAACAAGACACTGCTTCTGCCAATTTTTTATAATGTGGACCCCTCTCATGTGCGACATCAAAAGGGAAGTTTTGCAGAAGCCTTCACTAAGCATGAAGAGAGGTATAGGCAAGGCAATGATGATGCAGAGAAGGTGAAGAAGTGGAGAGCTGCTTTAACACAAGTGGCCAATCTCTCTGGGTTGGGTTCAAACAAATTTGGGTCTGACAGAGAACTTGTCCAACGCATTGTGGAACTTGTGCGCCGTGATGTAAGGCCTATCTCAATAATGTCGAGCAGCAATTTTGGAGCATTTGAATCAACAAGAAAAGCCATGAATGAGCTGATGGAGGCATTAGAAGATGATGAGGTCGCTGCCATTGGGGTCTATGGAATGGGAGGGGTTGGGAAGACAACCATGGTGAAACATGTCGGTGCACAAGCCCAAAAAATGGGGCGTTTTGATCGTGTGATTATGGCTGTTGTATCCCAAACCCCAGATACAAGAAATATTCAAGGCGCACTTGCGGATCTGCTGGGCTTGAAATTGGTGGAGGAGTCAGAAAGTGGACGAGCCGATAGATTGCAGACGGTGATACTTGGAAGAAATAAGATCCTTATAATCTTAGACGACATTTGGAATACACTTGACTTGTCAAGCATTGGAATTCCCAGCTACAACGAGCTTCAAATATGCAATTCCAAACTGCTATTCACCACAAGGAGATTGAATGTTTGCCATACCATGGAGAGCCAAGCAAAAATTCATCTCAATATCTTATCAGAAGATGATGCTTGGAACTTGTTTTTGAAGAAGGCAAGAAGGTCTTTTCAAAAGTCTAGCCCTTTCTATAAAGTAGCCAGAAAGGTAGCTAGAGAATGTGCTGGTCTCCCAGTTGCATTGATTGCAGTTGCGAGGGCACTTGGAGATAAAGAGCTTGAAGACTGGAAAGAAGCAGCCCGACAACTTGAGATGTCTCAACCTGCCAACCTTGACGAGGAGAAAGATGTGTTCAAGTGCATTAAGTTGAGCTATGATTACCTTGACAGCGACGACGCCAAGTCTTGCTTCTTGCTTTGCTGCCTATTCCCAGAAGATTATGACATCCGTGTTGAAGACTTGATGAGCTATGGGTTCGGAACAGGGTTGTTTCGACATGCCAACAGCACAATAGAATATGCCAGAGACAGAGCACATTCGGTTACTAGATACCTTATAGCTTCTTGTCTGCTTTTGGATGGGATAGAAGAAGGATATGTAAGGATGCATGATGTCATTCGAGATATGGCCATATCCATTGCATTCTCTGTAGATGGCCACCCCTCTTTGGTGAAAACAAATTGTCATTTGAAAGATTGGTCGATGGATGCGGACGAAGGCTACTCAGCAATCTCACTAATCGAGAGCAACATTTGCAAGCTTCCCAACAAGTTGGTATGTTCAAAGCTTCAGATTTTATTGCTACAAAACAATGCTGATATAGGAGAGATCCCAATAACCTTTTTTCAAAGCCCCAATGATTTAAGGGTCTTAGATCTTAGTTACACTGGCATTTCATCACTGCCCCAATCATTCAGTCTCCTAACCAGACTCCAAGCTTTGCATTTAGATGATTGTCTTTGCATGATTGACATATCCCTACTTGGAAAACTGAAAAAGCTTGAGATTCTTAGTATGAGACGCTGTTACTCTATTAAGAAATTTCCAAAAGAGATTGGAAATTTGACGAATTTAAGAATGTTGGACTTGACTGGAACCCGTTTTAAGACAGTTCCCTCTCAAGTGATATCAAGGTTGTATAGATTGGAAGAACTGTACATGAGATGTTATTTTCGCAAGTGGGGTTGTAAAGCTGCGGGGGGCTGGTTGCCTTTTCTTTGTAAGAATGCTAGCTTTGATGAGTTAATTGGCTTGTCAAGTTTAAACACTTTGCAGGTTGCCGTATGTGGTGCAGAATGCTTGCCTCAAAACGTTGAGTTCAATCCAAATTGGGACAGATTTTGTATAATTATCCAAAGATACGGCACGATGAGAGTACCCTCTTGGGACAATTTGGGCATTTTTGACGAGGTCTTCCTACATCGTCCTTCAAGAACATTGATCATGGCCGAAACCATGAATAACTTACCCGACTGGTTCATCAGTGTAGTGACAGAAAGAACAGATAATTTATGGTACAGAGATCGCCATGGCTTAACTAACCTTCTCGAGGAACACGACCGTGGGAGATTACATGGACTCAAGTGTTTGTGTATAGAATCTTCCGAATATGCCAAAGTGTTGTTGAACACAATAACATGGACTAGGGTGTCAAATGAACCTGTGTTTGAGGACTTGGAAGAGTTGGAAATCGCCGTGTGTCTCTTGGAGGAGCTATGTGTCGGTGAATTACCACCTGGCTCTCTACGTAATCTTAAGAAATTGATCGTGCTTAAGTGTAATTGGTTGGTGAAACCGCTTTTACCGTCAAAGTTGTTGCACAGATTAGAGAGTCTGGAAGAACTGTGTTGTTCTCATATGCGGGCAATGAAATATGTGTTTGGATTTGAAGGGCATGAACCACAACACGTTGTCTTGACGAGGCTGAGAATGATTTCATTGAGATCCATGAACAGCTTAATAAACATATGGGATGGTCCTGCTCCATCTGGAATCTTCCAGAGTCTCAAAATCTTTTCCTTGTTTAATTGCCAGAAACTGGAATATCTCTTCACATCTGATGTAGCTCGATGTCTTTTGCAATTGGAAGACGTTTTGGTAGAGGATTGCGATAGCTTGGACAGAATAATTGAAGCGAGTGAGGAAATAGAGAGCAACAAGATCGTTCTTCCACAGTTGAAGAACTTGGTTTTAAAAGATCTTCCAAAGCTCACAAGGCTGTCCAGTACAAGCAGCAGTACTATTGATATTGAAATCGAGTGCCCTACATTGGAACGCTTATATGTCTCTGCGTGCCGCCAGTTATCAATCTCTGCTTCTGATTTCAACAGCAGCAACGAAGTCCAACTAGATGATTACCTGCATTGGGCCACACTACGGTACAGGATCGACGACGTGAGTTACCACTCAGACTTTCTACGTGCGATAAGGTATGTCTCTGCGTGCCCCCAGTTATCAATCTCTGCTTCTGATTTCAACAGCAGCAACGAAGTCCAACTAGATGATTACCAGCATTGGGCCACACTACGGTACAGGATCGACGACGTGAGTTGCCACTCAGACTTTCTACGTGCGATAAGGTATGTCTCCATGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

1179

Amino Acids

133.91

Weight (kDa)

5.85

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 189 2.4e-52 TIR domain
TIR_2 PF13676 19 - 116 5e-17 TIR domain
NB-ARC PF00931 204 - 368 1.3e-32 NB-ARC domain
LRR_8 PF13855 576 - 633 6.7e-09 Leucine rich repeat
LRR_14 PF23598 628 - 702 1.7e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 873 - 1018 1.4e-23 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1008 - 1083 6.9e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000072)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38920
fragaria_vesca FvH4_1g15990 FvH4_1g20960 FvH4_1g24221 FvH4_1g24420 FvH4_2g01203 FvH4_2g08721 FvH4_2g09751 FvH4_2g13111 FvH4_2g14361 FvH4_2g19531 FvH4_2g20801 FvH4_2g36230 FvH4_3g07262 FvH4_3g14231 FvH4_3g21341 FvH4_3g23591 FvH4_3g27972 FvH4_3g28692 FvH4_4g05541 FvH4_4g07351 FvH4_4g07410 FvH4_4g13062 FvH4_4g15487 FvH4_4g15790 FvH4_4g17671 FvH4_4g26583 FvH4_4g28900 FvH4_4g31671 FvH4_5g25821 FvH4_5g31851 FvH4_5g38981 FvH4_6g29902 FvH4_7g03001 FvH4_7g10254
malus_domestica MD03G1147200.v1.1 MD15G1309200.v1.1 MD17G1150900.v1.1
prunus_persica Prupe.1G205500_v2.0.a1 Prupe.2G069500_v2.0.a1 Prupe.2G082600_v2.0.a1 Prupe.3G063400_v2.0.a1 Prupe.3G126800_v2.0.a1 Prupe.3G133900_v2.0.a1 Prupe.3G283300_v2.0.a1 Prupe.4G143500_v2.0.a1 Prupe.4G186900_v2.0.a1 Prupe.6G204400_v2.0.a1 Prupe.6G272800_v2.0.a1 Prupe.7G035500_v2.0.a1 Prupe.7G038400_v2.0.a1 Prupe.7G049300_v2.0.a1 Prupe.7G076800_v2.0.a1 Prupe.8G099500_v2.0.a1 Prupe.8G101300_v2.0.a1
pyrus_communis pycom03g10120 pycom04g16660 pycom08g19920 pycom11g21950 pycom14g01510 pycom14g06470
rosa_chinensis RchiOBHm_Chr1g0323941 RchiOBHm_Chr1g0325331 RchiOBHm_Chr1g0339311 RchiOBHm_Chr1g0345991 RchiOBHm_Chr1g0354891 RchiOBHm_Chr2g0118041 RchiOBHm_Chr3g0468731 RchiOBHm_Chr3g0488841 RchiOBHm_Chr4g0388981 RchiOBHm_Chr4g0398671 RchiOBHm_Chr4g0400431 RchiOBHm_Chr4g0402201 RchiOBHm_Chr4g0430711 RchiOBHm_Chr4g0432871 RchiOBHm_Chr4g0437761 RchiOBHm_Chr5g0066251 RchiOBHm_Chr5g0078071 RchiOBHm_Chr6g0279961 RchiOBHm_Chr6g0289791 RchiOBHm_Chr7g0187741 RchiOBHm_Chr7g0208481
rosa_laevigata RLG00000002376 RLG00000011026 RLG00000011403 RLG00000012646 RLG00000014435 RLG00000014869 RLG00000015450 RLG00000015464 RLG00000016451 RLG00000017121 RLG00000018788 RLG00000018819 RLG00000021054 RLG00000028424 RLG00000030071 RLG00000031242 RLG00000031840 RLG00000031955 RLG00000033437 RLG00000033571 RLG00000034722 RLG00000035706 RLG00000035774 RLG00000036341 RLG00000036639
rosa_multiflora Rmu_co8023178.1_g000001 Rmu_co8119834.1_g000001 Rmu_co8158796.1_g000001 Rmu_co8339863.1_g000001 Rmu_co8512857.1_g000001 Rmu_sc0000174.1_g000006 Rmu_sc0000206.1_g000002 Rmu_sc0000266.1_g000002 Rmu_sc0000361.1_g000026 Rmu_sc0000493.1_g000035 Rmu_sc0000600.1_g000057 Rmu_sc0000796.1_g000012 Rmu_sc0000882.1_g000045 Rmu_sc0000913.1_g000014 Rmu_sc0000946.1_g000041 Rmu_sc0000966.1_g000013 Rmu_sc0001018.1_g000024 Rmu_sc0001018.1_g000025 Rmu_sc0001159.1_g000043 Rmu_sc0001287.1_g000024 Rmu_sc0001291.1_g000041 Rmu_sc0001380.1_g000028 Rmu_sc0001767.1_g000008 Rmu_sc0001802.1_g000010 Rmu_sc0001824.1_g000009 Rmu_sc0001850.1_g000024 Rmu_sc0002105.1_g000011 Rmu_sc0002129.1_g000032 Rmu_sc0002845.1_g000002 Rmu_sc0003054.1_g000001 Rmu_sc0003063.1_g000035 Rmu_sc0003131.1_g000034 Rmu_sc0003303.1_g000022 Rmu_sc0004347.1_g000013 Rmu_sc0004521.1_g000015 Rmu_sc0004618.1_g000056 Rmu_sc0004757.1_g000013 Rmu_sc0005056.1_g000003 Rmu_sc0005354.1_g000011 Rmu_sc0005385.1_g000018 Rmu_sc0005928.1_g000007 Rmu_sc0005933.1_g000003 Rmu_sc0006223.1_g000044 Rmu_sc0006314.1_g000007 Rmu_sc0006399.1_g000008 Rmu_sc0006847.1_g000001 Rmu_sc0007698.1_g000004 Rmu_sc0008509.1_g000023 Rmu_sc0008804.1_g000010 Rmu_sc0008818.1_g000001 Rmu_sc0009489.1_g000001 Rmu_sc0010407.1_g000001 Rmu_sc0010532.1_g000006 Rmu_sc0010988.1_g000001 Rmu_sc0012343.1_g000003 Rmu_sc0012922.1_g000001 Rmu_sc0013078.1_g000009 Rmu_sc0013419.1_g000005 Rmu_sc0013453.1_g000002 Rmu_sc0017867.1_g000004 Rmu_sc0023349.1_g000001 Rmu_sc0025859.1_g000001 Rmu_sc0028153.1_g000002 Rmu_sc0029528.1_g000001 Rmu_sc0030464.1_g000001 Rmu_sc0035933.1_g000001 Rmu_sc0038973.1_g000001 Rmu_sc0040007.1_g000003 Rmu_ssc0000053.1_g000007 Rmu_ssc0000128.1_g000001
rosa_roxburghii Rroxscaffold_175G00431900 Rroxscaffold_1G00018370 Rroxscaffold_1G00035430 Rroxscaffold_1G00040660 Rroxscaffold_1G00048390 Rroxscaffold_1G00048520 Rroxscaffold_2G00078960 Rroxscaffold_2G00086820 Rroxscaffold_2G00112940 Rroxscaffold_2G00118350 Rroxscaffold_2G00139200 Rroxscaffold_2G00141770 Rroxscaffold_3G00221980 Rroxscaffold_3G00227730 Rroxscaffold_3G00241010 Rroxscaffold_3G00242660 Rroxscaffold_4G00305460 Rroxscaffold_4G00324810 Rroxscaffold_4G00326090 Rroxscaffold_5G00334080 Rroxscaffold_5G00335320 Rroxscaffold_5G00344260 Rroxscaffold_5G00348290 Rroxscaffold_5G00381300 Rroxscaffold_6G00392050 Rroxscaffold_6G00401420 Rroxscaffold_6G00405930 Rroxscaffold_6G00418270 Rroxscaffold_6G00421490 Rroxscaffold_7G00167580 Rroxscaffold_7G00172500 Rroxscaffold_7G00173600 Rroxscaffold_7G00185890 Rroxscaffold_7G00192320 Rroxscaffold_7G00197110 Rroxscaffold_7G00209660 Rroxscaffold_7G00211150 Rroxscaffold_7G00216650
rosa_rugosa Rorug01G0040600 Rorug01G0063600 Rorug01G0071700 Rorug01G0184500 Rorug01G0302600 Rorug01G0322600 Rorug01G0403900 Rorug02G0026600 Rorug02G0026700 Rorug02G0074300 Rorug02G0074400 Rorug02G0074500.1 Rorug02G0326900 Rorug02G0351800 Rorug02G0378400 Rorug02G0473800 Rorug03G0023400 Rorug03G0073100 Rorug03G0074900 Rorug03G0118200 Rorug03G0129800 Rorug03G0150100 Rorug03G0178300 Rorug03G0192600 Rorug03G0201700 Rorug03G0231800 Rorug03G0271300 Rorug03G0360000.1 Rorug04G0026900 Rorug04G0036300 Rorug04G0081200 Rorug04G0129600 Rorug04G0153600 Rorug04G0169000 Rorug04G0211000 Rorug04G0211000 Rorug04G0440300 Rorug05G0008100 Rorug05G0083200 Rorug05G0137000 Rorug05G0251200 Rorug05G0411900 Rorug05G0422200 Rorug05G0472700 Rorug06G0026700 Rorug06G0062900 Rorug06G0063000 Rorug06G0069800 Rorug06G0081100 Rorug06G0093500 Rorug06G0095300 Rorug06G0241900 Rorug06G0480400 Rorug07G0043700 Rorug07G0113100 Rorug07G0211300.1 Rorug07G0267700 Rorug07G0281900 Rorug07G0307100
rosa_samantha Rh1AG057800 Rh1AG058600 Rh1AG167200 Rh1AG312300 Rh1AG364500 Rh1BG048900 Rh1BG120100 Rh1BG275500 Rh1BG351000 Rh1CG060500 Rh1CG060600 Rh1DG064500 Rh2AG116500 Rh2BG281500 Rh2BG454900 Rh2BG458000 Rh2BG661700 Rh2CG270000 Rh2DG031300 Rh2DG465000 Rh3DG087300 Rh4AG000500 Rh4AG015500 Rh4AG212800 Rh4BG073500 Rh4BG077400 Rh4BG096800 Rh4BG390300 Rh4CG238100 Rh4CG325900 Rh5AG150200 Rh5CG253600 Rh5DG148600 Rh6AG397200 Rh6BG405900 Rh7BG108000 Rh7BG438000 Rh7CG128200 Rh7CG286000 Rh7CG372000 Rh7CG454900
rosa_wichuraiana Rw1G016000 Rw2G002480 Rw3G029030 Rw4G012010 Rw4G019280 Rw4G020280 Rw4G026190 Rw7G009640 Rw7G020950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 333, 902
AasI GACNNNNNNGTC 1 cut(s) 2590
Acc36I ACCTGC 3 cut(s) 1273, 2206, 3309
AccB7I CCANNNNNTGG 3 cut(s) 733, 1027, 2980
AciI CCGC 6 cut(s) 809, 1652, 2138, 2738, 2803, 3238
AclI AACGTT 1 cut(s) 2253
AclWI GGATC 6 cut(s) 819, 889, 1765, 2880, 3335, 3485
AcoI YGGCCR 4 cut(s) 468, 1569, 1597, 2388
AcsI RAATTY 6 cut(s) 497, 948, 1044, 1982, 2002, 2011
AcuI CTGAAG 4 cut(s) 52, 146, 318, 1715
AcyI GRCGYC 1 cut(s) 1338
AfaI GTAC 7 cut(s) 2099, 2319, 2459, 3169, 3181, 3323, 3473
AflII CTTAAG 2 cut(s) 2696, 2714
AflIII ACRYGT 2 cut(s) 709, 2848
AhdI GACNNNNNGTC 1 cut(s) 1342
AjiI CACGTC 2 cut(s) 3337, 3487
AjnI CCWGG 2 cut(s) 212, 2677
AjuI GAANNNNNNNTTGG 8 cut(s) 232, 264, 977, 1009, 1029, 1061, 1334, 1366
AleI CACNNNNGTG 1 cut(s) 2502
Alw21I GWGCWC 3 cut(s) 14, 721, 1477
Alw44I GTGCAC 1 cut(s) 717
AlwI GGATC 6 cut(s) 819, 889, 1765, 2880, 3335, 3485
AlwNI CAGNNNCTG 1 cut(s) 2980
Ama87I CYCGRG 2 cut(s) 7, 2489
AoxI GGCC 8 cut(s) 3, 468, 557, 1569, 1597, 2388, 3309, 3459
ApaLI GTGCAC 1 cut(s) 717
ApeKI GCWGC 9 cut(s) 452, 579, 656, 1238, 1362, 2135, 3174, 3273, 3423
ApoI RAATTY 6 cut(s) 497, 948, 1044, 1982, 2002, 2011
Asp700I GAANNNNTTC 1 cut(s) 2875
AspLEI GCGC 2 cut(s) 546, 803
AspS9I GGNCC 4 cut(s) 340, 2918, 3309, 3459
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 8 cut(s) 86, 448, 715, 886, 988, 1624, 2678, 2743
AsuNHI GCTAGC 1 cut(s) 2171
AvaI CYCGRG 2 cut(s) 7, 2489
AvaII GGWCC 2 cut(s) 340, 2918
BaeGI GKGCMC 4 cut(s) 721, 1204, 3206, 3389
BalI TGGCCA 3 cut(s) 470, 1571, 1599
BanII GRGCYC 1 cut(s) 14
BarI GAAGNNNNNNTAC 2 cut(s) 2527, 2559
BbsI GAAGAC 6 cut(s) 84, 698, 1230, 1404, 2347, 3036
Bbv12I GWGCWC 3 cut(s) 14, 721, 1477
BbvI GCAGC 9 cut(s) 439, 591, 643, 1250, 1349, 2122, 3186, 3285, 3435
BccI CCATC 6 cut(s) 622, 1514, 1589, 1639, 2909, 2935
BceAI ACGGC 4 cut(s) 531, 2207, 2320, 2621
BcgI CGANNNNNNTGC 2 cut(s) 3085, 3119
BciT130I CCWGG 2 cut(s) 214, 2679
BciVI GTATCC 2 cut(s) 109, 778
BclI TGATCA 1 cut(s) 2382
BcnI CCSGG 1 cut(s) 85
BfaI CTAG 7 cut(s) 1122, 1155, 1488, 2172, 2586, 3290, 3440
BfmI CTRYAG 1 cut(s) 1590
BfrI CTTAAG 2 cut(s) 2696, 2714
BfuAI ACCTGC 3 cut(s) 1273, 2206, 3309
BfuI GTATCC 2 cut(s) 109, 778
BglII AGATCT 2 cut(s) 1816, 3139
BmcAI AGTACT 1 cut(s) 3181
Bme1390I CCNGG 3 cut(s) 85, 214, 2679
Bme18I GGWCC 2 cut(s) 340, 2918
BmeRI GACNNNNNGTC 1 cut(s) 1342
BmeT110I CYCGRG 2 cut(s) 7, 2489
BmgBI CACGTC 2 cut(s) 3337, 3487
BmgT120I GGNCC 4 cut(s) 340, 2918, 3309, 3459
BmiI GGNNCC 2 cut(s) 342, 2038
BmrFI CCNGG 3 cut(s) 85, 214, 2679
BmrI ACTGGG 2 cut(s) 1169, 3385
BmsI GCATC 5 cut(s) 162, 415, 1063, 1536, 1639
BmtI GCTAGC 1 cut(s) 2175
BmuI ACTGGG 2 cut(s) 1169, 3385
BpiI GAAGAC 6 cut(s) 84, 698, 1230, 1404, 2347, 3036
BpuEI CTTGAG 4 cut(s) 1271, 1964, 2046, 2507
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 3 cut(s) 2690, 3362, 3512
BsaHI GRCGYC 1 cut(s) 1338
BsaI GGTCTC 1 cut(s) 1175
BsaJI CCNNGG 5 cut(s) 154, 699, 1026, 2470, 2503
Bse1I ACTGG 9 cut(s) 1175, 1232, 1834, 2038, 2420, 2985, 3165, 3241, 3391
Bse3DI GCAATG 3 cut(s) 424, 1578, 2814
BseBI CCWGG 2 cut(s) 214, 2679
BseDI CCNNGG 5 cut(s) 154, 699, 1026, 2470, 2503
BseGI GGATG 8 cut(s) 154, 177, 274, 1386, 1525, 1551, 1654, 2920
BseMI GCAATG 3 cut(s) 424, 1578, 2814
BseMII CTCAG 6 cut(s) 53, 76, 1680, 2858, 3363, 3513
BseNI ACTGG 9 cut(s) 1175, 1232, 1834, 2038, 2420, 2985, 3165, 3241, 3391
BseRI GAGGAG 4 cut(s) 27, 851, 1295, 2666
BseSI GKGCMC 4 cut(s) 721, 1204, 3206, 3389
BseXI GCAGC 9 cut(s) 439, 591, 643, 1250, 1349, 2122, 3186, 3285, 3435
BseYI CCCAGC 2 cut(s) 817, 953
BsgI GTGCAG 2 cut(s) 240, 2253
Bsh1285I CGRYCG 1 cut(s) 2503
BshFI GGCC 8 cut(s) 5, 470, 559, 1571, 1599, 2390, 3311, 3461
BsiEI CGRYCG 1 cut(s) 2503
BsiHKAI GWGCWC 3 cut(s) 14, 721, 1477
BsiHKCI CYCGRG 2 cut(s) 7, 2489
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 2 cut(s) 2288, 2342
BsmBI CGTCTC 1 cut(s) 1954
BsmFI GGGAC 2 cut(s) 2288, 2342
BsmI GAATGC 3 cut(s) 1583, 2173, 2243
BsnI GGCC 8 cut(s) 5, 470, 559, 1571, 1599, 2390, 3311, 3461
Bso31I GGTCTC 1 cut(s) 1175
BsoBI CYCGRG 2 cut(s) 7, 2489
Bsp1286I GDGCHC 6 cut(s) 14, 721, 1204, 1477, 3206, 3389
Bsp1407I TGTACA 1 cut(s) 2097
Bsp19I CCATGG 3 cut(s) 699, 1026, 2470
BspACI CCGC 6 cut(s) 809, 1652, 2138, 2738, 2803, 3238
BspANI GGCC 8 cut(s) 5, 470, 559, 1571, 1599, 2390, 3311, 3461
BspCNI CTCAG 6 cut(s) 52, 75, 1679, 2859, 3362, 3512
BspLI GGNNCC 2 cut(s) 342, 2038
BspMI ACCTGC 3 cut(s) 1273, 2206, 3309
BspOI GCTAGC 1 cut(s) 2175
BspPI GGATC 6 cut(s) 819, 889, 1765, 2880, 3335, 3485
BspTI CTTAAG 2 cut(s) 2696, 2714
BspTNI GGTCTC 1 cut(s) 1175
BsrDI GCAATG 3 cut(s) 424, 1578, 2814
BsrGI TGTACA 1 cut(s) 2097
BsrI ACTGG 9 cut(s) 1175, 1232, 1834, 2038, 2420, 2985, 3165, 3241, 3391
BssECI CCNNGG 5 cut(s) 154, 699, 1026, 2470, 2503
BssNI GRCGYC 1 cut(s) 1338
BssT1I CCWWGG 4 cut(s) 154, 699, 1026, 2470
Bst2UI CCWGG 2 cut(s) 214, 2679
Bst4CI ACNGT 9 cut(s) 874, 2053, 2097, 2504, 2748, 2787, 3117, 3321, 3471
Bst6I CTCTTC 4 cut(s) 31, 393, 2616, 2996
BstACI GRCGYC 1 cut(s) 1338
BstAFI CTTAAG 2 cut(s) 2696, 2714
BstAPI GCANNNNNTGC 3 cut(s) 172, 1187, 1695
BstAUI TGTACA 1 cut(s) 2097
BstBAI YACGTR 3 cut(s) 2690, 3362, 3512
BstC8I GCNNGC 6 cut(s) 1700, 2173, 2243, 2805, 3235, 3385
BstDSI CCRYGG 4 cut(s) 699, 1026, 2470, 2503
BstENI CCTNNNNNAGG 2 cut(s) 1277, 2489
BstF5I GGATG 8 cut(s) 154, 177, 274, 1386, 1525, 1551, 1654, 2920
BstHHI GCGC 2 cut(s) 546, 803
BstMCI CGRYCG 1 cut(s) 2503
BstMWI GCNNNNNNNGC 4 cut(s) 172, 1187, 1695, 3157
BstNI CCWGG 2 cut(s) 214, 2679
BstNSI RCATGY 2 cut(s) 713, 1442
BstSCI CCNGG 3 cut(s) 83, 212, 2677
BstSFI CTRYAG 1 cut(s) 1590
BstSLI GKGCMC 4 cut(s) 721, 1204, 3206, 3389
BstSNI TACGTA 1 cut(s) 2690
BstV1I GCAGC 9 cut(s) 439, 591, 643, 1250, 1349, 2122, 3186, 3285, 3435
BstV2I GAAGAC 6 cut(s) 84, 698, 1230, 1404, 2347, 3036
BstX2I RGATCY 6 cut(s) 811, 894, 1770, 1816, 2885, 3139
BstXI CCANNNNNNTGG 1 cut(s) 1714
BstYI RGATCY 6 cut(s) 811, 894, 1770, 1816, 2885, 3139
BsuI GTATCC 2 cut(s) 109, 778
BsuRI GGCC 8 cut(s) 5, 470, 559, 1571, 1599, 2390, 3311, 3461
BtgI CCRYGG 4 cut(s) 699, 1026, 2470, 2503
BtrI CACGTC 2 cut(s) 3337, 3487
BtsCI GGATG 8 cut(s) 154, 177, 274, 1386, 1525, 1551, 1654, 2920
BtsI GCAGTG 2 cut(s) 311, 1841
BtsIMutI CAGTG 5 cut(s) 47, 311, 1827, 1841, 2431
BveI ACCTGC 3 cut(s) 1273, 2206, 3309
Cac8I GCNNGC 6 cut(s) 1700, 2173, 2243, 2805, 3235, 3385
CaiI CAGNNNCTG 1 cut(s) 2980
CfoI GCGC 2 cut(s) 546, 803
Cfr13I GGNCC 4 cut(s) 340, 2918, 3309, 3459
CseI GACGC 2 cut(s) 1346, 1971
Csp6I GTAC 7 cut(s) 2098, 2318, 2458, 3168, 3180, 3322, 3472
CviQI GTAC 7 cut(s) 2098, 2318, 2458, 3168, 3180, 3322, 3472
DraI TTTAAA 2 cut(s) 2205, 3135
DrdI GACNNNNNNGTC 1 cut(s) 2590
DriI GACNNNNNGTC 1 cut(s) 1342
DseDI GACNNNNNNGTC 1 cut(s) 2590
EaeI YGGCCR 4 cut(s) 468, 1569, 1597, 2388
Eam1104I CTCTTC 4 cut(s) 31, 393, 2616, 2996
Eam1105I GACNNNNNGTC 1 cut(s) 1342
EarI CTCTTC 4 cut(s) 31, 393, 2616, 2996
Ecl136II GAGCTC 1 cut(s) 12
Eco105I TACGTA 1 cut(s) 2690
Eco130I CCWWGG 4 cut(s) 154, 699, 1026, 2470
Eco147I AGGCCT 1 cut(s) 559
Eco24I GRGCYC 1 cut(s) 14
Eco31I GGTCTC 1 cut(s) 1175
Eco32I GATATC 1 cut(s) 2070
Eco47I GGWCC 2 cut(s) 340, 2918
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 4 cut(s) 52, 146, 318, 1715
Eco88I CYCGRG 2 cut(s) 7, 2489
EcoICRI GAGCTC 1 cut(s) 12
EcoNI CCTNNNNNAGG 2 cut(s) 1277, 2489
EcoRI GAATTC 1 cut(s) 948
EcoRII CCWGG 2 cut(s) 212, 2677
EcoRV GATATC 1 cut(s) 2070
EcoT14I CCWWGG 4 cut(s) 154, 699, 1026, 2470
EcoT22I ATGCAT 1 cut(s) 1551
EcoT38I GRGCYC 1 cut(s) 14
ErhI CCWWGG 4 cut(s) 154, 699, 1026, 2470
Esp3I CGTCTC 1 cut(s) 1954
FalI AAGNNNNNCTT 6 cut(s) 789, 821, 1093, 1125, 1334, 1366
FaqI GGGAC 2 cut(s) 2288, 2342
FauI CCCGC 2 cut(s) 2131, 2796
FauNDI CATATG 2 cut(s) 2799, 2909
FbaI TGATCA 1 cut(s) 2382
FokI GGATG 8 cut(s) 161, 184, 281, 1373, 1532, 1558, 1661, 2927
FriOI GRGCYC 1 cut(s) 14
FspBI CTAG 7 cut(s) 1122, 1155, 1488, 2172, 2586, 3290, 3440
GlaI GCGC 2 cut(s) 545, 802
GsaI CCCAGC 2 cut(s) 821, 957
HaeIII GGCC 8 cut(s) 5, 470, 559, 1571, 1599, 2390, 3311, 3461
HapII CCGG 1 cut(s) 85
HgaI GACGC 2 cut(s) 1346, 1971
HhaI GCGC 2 cut(s) 546, 803
Hin1I GRCGYC 1 cut(s) 1338
Hin6I GCGC 2 cut(s) 544, 801
HinP1I GCGC 2 cut(s) 544, 801
HindIII AAGCTT 4 cut(s) 1700, 1727, 1878, 1940
HpaII CCGG 1 cut(s) 85
HphI GGTGA 8 cut(s) 86, 448, 715, 886, 988, 1624, 2678, 2743
Hpy166II GTNNAC 3 cut(s) 340, 719, 851
Hpy188III TCNNGA 8 cut(s) 1562, 1684, 2373, 2489, 2777, 2857, 2931, 2941
Hpy8I GTNNAC 3 cut(s) 340, 719, 851
Hpy99I CGWCG 6 cut(s) 1337, 1340, 3335, 3338, 3485, 3488
HpyCH4III ACNGT 9 cut(s) 874, 2053, 2097, 2504, 2748, 2787, 3117, 3321, 3471
HpyCH4IV ACGT 8 cut(s) 2253, 2689, 2850, 3033, 3336, 3361, 3486, 3511
HpyF10VI GCNNNNNNNGC 4 cut(s) 172, 1187, 1695, 3157
HpySE526I ACGT 8 cut(s) 2253, 2689, 2850, 3033, 3336, 3361, 3486, 3511
Hsp92I GRCGYC 1 cut(s) 1338
HspAI GCGC 2 cut(s) 544, 801
Ksp22I TGATCA 1 cut(s) 2382
LmnI GCTCC 5 cut(s) 17, 40, 590, 2653, 2929
Lsp1109I GCAGC 9 cut(s) 439, 591, 643, 1250, 1349, 2122, 3186, 3285, 3435
LweI GCATC 5 cut(s) 162, 415, 1063, 1536, 1639
MaeI CTAG 7 cut(s) 1122, 1155, 1488, 2172, 2586, 3290, 3440
MaeII ACGT 8 cut(s) 2253, 2689, 2850, 3033, 3336, 3361, 3486, 3511
MaeIII GTNAC 5 cut(s) 1483, 1823, 1967, 2431, 3341
MfeI CAATTG 2 cut(s) 201, 3023
MflI RGATCY 6 cut(s) 811, 894, 1770, 1816, 2885, 3139
MhlI GDGCHC 6 cut(s) 14, 721, 1204, 1477, 3206, 3389
MlsI TGGCCA 3 cut(s) 470, 1571, 1599
MluNI TGGCCA 3 cut(s) 470, 1571, 1599
MlyI GAGTC 5 cut(s) 848, 1866, 2514, 2782, 2953
MmeI TCCRAC 5 cut(s) 547, 2004, 2607, 3310, 3460
Mox20I TGGCCA 3 cut(s) 470, 1571, 1599
Mph1103I ATGCAT 1 cut(s) 1551
MroXI GAANNNNTTC 1 cut(s) 2875
MscI TGGCCA 3 cut(s) 470, 1571, 1599
MslI CAYNNNNRTG 3 cut(s) 714, 1890, 2502
Msp20I TGGCCA 3 cut(s) 470, 1571, 1599
MspCI CTTAAG 2 cut(s) 2696, 2714
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 3 cut(s) 85, 214, 2679
MssI GTTTAAAC 1 cut(s) 2205
MunI CAATTG 2 cut(s) 201, 3023
Mva1269I GAATGC 3 cut(s) 1583, 2173, 2243
MvaI CCWGG 2 cut(s) 214, 2679
MwoI GCNNNNNNNGC 4 cut(s) 172, 1187, 1695, 3157
NciI CCSGG 1 cut(s) 85
NcoI CCATGG 3 cut(s) 699, 1026, 2470
NdeI CATATG 2 cut(s) 2799, 2909
NheI GCTAGC 1 cut(s) 2171
NlaIV GGNNCC 2 cut(s) 342, 2038
NmuCI GTSAC 1 cut(s) 2431
NsiI ATGCAT 1 cut(s) 1551
NspI RCATGY 2 cut(s) 713, 1442
OliI CACNNNNGTG 1 cut(s) 2502
PaeR7I CTCGAG 2 cut(s) 7, 2489
PceI AGGCCT 1 cut(s) 559
PciI ACATGT 1 cut(s) 709
PcsI WCGNNNNNNNCGW 1 cut(s) 2496
PctI GAATGC 3 cut(s) 1583, 2173, 2243
PdmI GAANNNNTTC 1 cut(s) 2875
PfeI GAWTC 6 cut(s) 209, 283, 599, 1948, 2540, 2934
PflFI GACNNNGTC 1 cut(s) 2351
PflMI CCANNNNNTGG 3 cut(s) 733, 1027, 2980
PleI GAGTC 5 cut(s) 847, 1866, 2514, 2781, 2952
PmeI GTTTAAAC 1 cut(s) 2205
PpsI GAGTC 5 cut(s) 847, 1866, 2514, 2781, 2952
Ppu21I YACGTR 3 cut(s) 2690, 3362, 3512
PscI ACATGT 1 cut(s) 709
PsiI TTATAA 2 cut(s) 333, 902
Psp124BI GAGCTC 1 cut(s) 14
Psp1406I AACGTT 1 cut(s) 2253
Psp6I CCWGG 2 cut(s) 212, 2677
PspFI CCCAGC 2 cut(s) 817, 953
PspGI CCWGG 2 cut(s) 212, 2677
PspN4I GGNNCC 2 cut(s) 342, 2038
PspPI GGNCC 4 cut(s) 340, 2918, 3309, 3459
PspXI VCTCGAGB 1 cut(s) 7
PstNI CAGNNNCTG 1 cut(s) 2980
PsuI RGATCY 6 cut(s) 811, 894, 1770, 1816, 2885, 3139
PsyI GACNNNGTC 1 cut(s) 2351
RsaI GTAC 7 cut(s) 2099, 2319, 2459, 3169, 3181, 3323, 3473
RsaNI GTAC 7 cut(s) 2098, 2318, 2458, 3168, 3180, 3322, 3472
RseI CAYNNNNRTG 3 cut(s) 714, 1890, 2502
SacI GAGCTC 1 cut(s) 14
Sau96I GGNCC 4 cut(s) 340, 2918, 3309, 3459
ScaI AGTACT 1 cut(s) 3181
SchI GAGTC 5 cut(s) 848, 1866, 2514, 2782, 2953
ScrFI CCNGG 3 cut(s) 85, 214, 2679
SduI GDGCHC 6 cut(s) 14, 721, 1204, 1477, 3206, 3389
SfaNI GCATC 5 cut(s) 162, 415, 1063, 1536, 1639
SfcI CTRYAG 1 cut(s) 1590
Sfr274I CTCGAG 2 cut(s) 7, 2489
SinI GGWCC 2 cut(s) 340, 2918
SlaI CTCGAG 2 cut(s) 7, 2489
SmiMI CAYNNNNRTG 3 cut(s) 714, 1890, 2502
SmlI CTYRAG 8 cut(s) 7, 1250, 1943, 2061, 2489, 2522, 2696, 2714
SmoI CTYRAG 8 cut(s) 7, 1250, 1943, 2061, 2489, 2522, 2696, 2714
SnaBI TACGTA 1 cut(s) 2690
SseBI AGGCCT 1 cut(s) 559
SsiI CCGC 6 cut(s) 809, 1652, 2138, 2738, 2803, 3238
SspI AATATT 1 cut(s) 793
SspMI CTAG 7 cut(s) 1122, 1155, 1488, 2172, 2586, 3290, 3440
SstI GAGCTC 1 cut(s) 14
StuI AGGCCT 1 cut(s) 559
StyD4I CCNGG 3 cut(s) 83, 212, 2677
StyI CCWWGG 4 cut(s) 154, 699, 1026, 2470
TaaI ACNGT 9 cut(s) 874, 2053, 2097, 2504, 2748, 2787, 3117, 3321, 3471
TaiI ACGT 8 cut(s) 2256, 2692, 2853, 3036, 3339, 3364, 3489, 3514
TatI WGTACW 3 cut(s) 2097, 3167, 3179
TauI GCSGC 1 cut(s) 3240
TfiI GAWTC 6 cut(s) 209, 283, 599, 1948, 2540, 2934
TscAI CASTG 5 cut(s) 47, 318, 1834, 1848, 2431
TseFI GTSAC 1 cut(s) 2431
TseI GCWGC 9 cut(s) 452, 579, 656, 1238, 1362, 2135, 3174, 3273, 3423
Tsp45I GTSAC 1 cut(s) 2431
TspGWI ACGGA 1 cut(s) 1379
TspRI CASTG 5 cut(s) 47, 318, 1834, 1848, 2431
Tth111I GACNNNGTC 1 cut(s) 2351
Van91I CCANNNNNTGG 3 cut(s) 733, 1027, 2980
Vha464I CTTAAG 2 cut(s) 2696, 2714
VneI GTGCAC 1 cut(s) 717
VpaK11BI GGWCC 2 cut(s) 340, 2918
XagI CCTNNNNNAGG 2 cut(s) 1277, 2489
XapI RAATTY 6 cut(s) 497, 948, 1044, 1982, 2002, 2011
XceI RCATGY 2 cut(s) 713, 1442
XhoI CTCGAG 2 cut(s) 7, 2489
XmnI GAANNNNTTC 1 cut(s) 2875
XspI CTAG 7 cut(s) 1122, 1155, 1488, 2172, 2586, 3290, 3440
ZrmI AGTACT 1 cut(s) 3181
Zsp2I ATGCAT 1 cut(s) 1551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.