Reverse transcriptase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 152, 372 |
| AccII | CGCG | 1 cut(s) | 191 |
| AciI | CCGC | 2 cut(s) | 191, 403 |
| AdeI | CACNNNGTG | 1 cut(s) | 379 |
| AfaI | GTAC | 4 cut(s) | 26, 132, 415, 502 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 51, 95, 141, 215 |
| AgsI | TTSAA | 2 cut(s) | 308, 341 |
| AjnI | CCWGG | 2 cut(s) | 375, 543 |
| AoxI | GGCC | 1 cut(s) | 558 |
| ApeKI | GCWGC | 2 cut(s) | 487, 529 |
| AspS9I | GGNCC | 1 cut(s) | 558 |
| AsuC2I | CCSGG | 1 cut(s) | 157 |
| AsuHPI | GGTGA | 1 cut(s) | 121 |
| BaeGI | GKGCMC | 1 cut(s) | 375 |
| BanI | GGYRCC | 2 cut(s) | 152, 372 |
| BbvI | GCAGC | 2 cut(s) | 499, 516 |
| BccI | CCATC | 1 cut(s) | 113 |
| BciT130I | CCWGG | 2 cut(s) | 377, 545 |
| BclI | TGATCA | 2 cut(s) | 387, 511 |
| BcnI | CCSGG | 1 cut(s) | 157 |
| BfaI | CTAG | 2 cut(s) | 231, 562 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 526 |
| BisI | GCNGC | 3 cut(s) | 404, 488, 530 |
| BlsI | GCNGC | 3 cut(s) | 405, 489, 531 |
| Bme1390I | CCNGG | 3 cut(s) | 157, 377, 545 |
| BmgT120I | GGNCC | 1 cut(s) | 558 |
| BmiI | GGNNCC | 2 cut(s) | 154, 374 |
| BmrFI | CCNGG | 3 cut(s) | 157, 377, 545 |
| BmsI | GCATC | 2 cut(s) | 304, 390 |
| Bpu10I | CCTNAGC | 1 cut(s) | 318 |
| BpuEI | CTTGAG | 1 cut(s) | 78 |
| BpuMI | CCSGG | 1 cut(s) | 157 |
| BsaJI | CCNNGG | 2 cut(s) | 156, 437 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 272, 302 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 51, 95, 141, 215 |
| BseBI | CCWGG | 2 cut(s) | 377, 545 |
| BseDI | CCNNGG | 2 cut(s) | 156, 437 |
| BseGI | GGATG | 1 cut(s) | 319 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 51, 95, 141, 215 |
| BseMII | CTCAG | 1 cut(s) | 332 |
| BseRI | GAGGAG | 1 cut(s) | 365 |
| BseSI | GKGCMC | 1 cut(s) | 375 |
| BseXI | GCAGC | 2 cut(s) | 499, 516 |
| Bsh1236I | CGCG | 1 cut(s) | 191 |
| BshFI | GGCC | 1 cut(s) | 560 |
| BshNI | GGYRCC | 2 cut(s) | 152, 372 |
| BsiSI | CCGG | 1 cut(s) | 156 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 51, 95, 141, 215 |
| BsnI | GGCC | 1 cut(s) | 560 |
| Bsp1286I | GDGCHC | 1 cut(s) | 375 |
| Bsp143I | GATC | 2 cut(s) | 387, 511 |
| BspACI | CCGC | 2 cut(s) | 191, 403 |
| BspANI | GGCC | 1 cut(s) | 560 |
| BspCNI | CTCAG | 1 cut(s) | 331 |
| BspFNI | CGCG | 1 cut(s) | 191 |
| BspLI | GGNNCC | 2 cut(s) | 154, 374 |
| BspT107I | GGYRCC | 2 cut(s) | 152, 372 |
| BssECI | CCNNGG | 2 cut(s) | 156, 437 |
| BssMI | GATC | 2 cut(s) | 387, 511 |
| BssT1I | CCWWGG | 1 cut(s) | 437 |
| Bst2UI | CCWGG | 2 cut(s) | 377, 545 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 476 |
| BstDEI | CTNAG | 1 cut(s) | 318 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 93 |
| BstF5I | GGATG | 1 cut(s) | 319 |
| BstFNI | CGCG | 1 cut(s) | 191 |
| BstKTI | GATC | 2 cut(s) | 390, 514 |
| BstMBI | GATC | 2 cut(s) | 387, 511 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 476, 526 |
| BstNI | CCWGG | 2 cut(s) | 377, 545 |
| BstNSI | RCATGY | 1 cut(s) | 239 |
| BstSCI | CCNGG | 3 cut(s) | 155, 375, 543 |
| BstSLI | GKGCMC | 1 cut(s) | 375 |
| BstUI | CGCG | 1 cut(s) | 191 |
| BstV1I | GCAGC | 2 cut(s) | 499, 516 |
| BsuRI | GGCC | 1 cut(s) | 560 |
| BtsCI | GGATG | 1 cut(s) | 319 |
| BtsI | GCAGTG | 1 cut(s) | 39 |
| BtsIMutI | CAGTG | 1 cut(s) | 39 |
| Cfr13I | GGNCC | 1 cut(s) | 558 |
| CsiI | ACCWGGT | 1 cut(s) | 375 |
| Csp6I | GTAC | 4 cut(s) | 25, 131, 414, 501 |
| CviAII | CATG | 3 cut(s) | 236, 258, 461 |
| CviJI | RGCY | 5 cut(s) | 99, 429, 529, 548, 560 |
| CviKI_1 | RGCY | 5 cut(s) | 99, 429, 529, 548, 560 |
| CviQI | GTAC | 4 cut(s) | 25, 131, 414, 501 |
| DdeI | CTNAG | 1 cut(s) | 318 |
| DpnI | GATC | 2 cut(s) | 389, 513 |
| DpnII | GATC | 2 cut(s) | 387, 511 |
| DraIII | CACNNNGTG | 1 cut(s) | 379 |
| Eco130I | CCWWGG | 1 cut(s) | 437 |
| Eco32I | GATATC | 1 cut(s) | 42 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 93 |
| EcoO109I | RGGNCCY | 1 cut(s) | 558 |
| EcoRII | CCWGG | 2 cut(s) | 375, 543 |
| EcoRV | GATATC | 1 cut(s) | 42 |
| EcoT14I | CCWWGG | 1 cut(s) | 437 |
| ErhI | CCWWGG | 1 cut(s) | 437 |
| FaeI | CATG | 3 cut(s) | 239, 261, 464 |
| FaiI | YATR | 9 cut(s) | 18, 237, 259, 291, 432, 462, 477, 526, 554 |
| FatI | CATG | 3 cut(s) | 235, 257, 460 |
| FbaI | TGATCA | 2 cut(s) | 387, 511 |
| Fnu4HI | GCNGC | 3 cut(s) | 404, 488, 530 |
| FokI | GGATG | 1 cut(s) | 326 |
| Fsp4HI | GCNGC | 3 cut(s) | 404, 488, 530 |
| FspBI | CTAG | 2 cut(s) | 231, 562 |
| GluI | GCNGC | 3 cut(s) | 404, 488, 530 |
| HaeIII | GGCC | 1 cut(s) | 560 |
| HapII | CCGG | 1 cut(s) | 156 |
| Hin1II | CATG | 3 cut(s) | 239, 261, 464 |
| HinfI | GANTC | 1 cut(s) | 308 |
| HpaII | CCGG | 1 cut(s) | 156 |
| HphI | GGTGA | 1 cut(s) | 121 |
| Hpy188I | TCNGA | 2 cut(s) | 313, 511 |
| HpyAV | CCTTC | 1 cut(s) | 99 |
| HpyCH4V | TGCA | 4 cut(s) | 125, 239, 362, 470 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 476, 526 |
| HpyF3I | CTNAG | 1 cut(s) | 318 |
| Hsp92II | CATG | 3 cut(s) | 239, 261, 464 |
| Ksp22I | TGATCA | 2 cut(s) | 387, 511 |
| Kzo9I | GATC | 2 cut(s) | 387, 511 |
| LmnI | GCTCC | 2 cut(s) | 56, 280 |
| LpnPI | CCDG | 9 cut(s) | 64, 127, 169, 305, 362, 389, 518, 530, 557 |
| Lsp1109I | GCAGC | 2 cut(s) | 499, 516 |
| LweI | GCATC | 2 cut(s) | 304, 390 |
| MabI | ACCWGGT | 1 cut(s) | 375 |
| MaeI | CTAG | 2 cut(s) | 231, 562 |
| MaeIII | GTNAC | 4 cut(s) | 220, 264, 491, 539 |
| MalI | GATC | 2 cut(s) | 389, 513 |
| MboI | GATC | 2 cut(s) | 387, 511 |
| MfeI | CAATTG | 2 cut(s) | 269, 363 |
| MhlI | GDGCHC | 1 cut(s) | 375 |
| MluCI | AATT | 3 cut(s) | 269, 336, 363 |
| MmeI | TCCRAC | 1 cut(s) | 258 |
| MnlI | CCTC | 4 cut(s) | 96, 173, 219, 343 |
| MseI | TTAA | 1 cut(s) | 105 |
| MspI | CCGG | 1 cut(s) | 156 |
| MspR9I | CCNGG | 3 cut(s) | 157, 377, 545 |
| MunI | CAATTG | 2 cut(s) | 269, 363 |
| MvaI | CCWGG | 2 cut(s) | 377, 545 |
| MvnI | CGCG | 1 cut(s) | 191 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 476, 526 |
| NciI | CCSGG | 1 cut(s) | 157 |
| NdeII | GATC | 2 cut(s) | 387, 511 |
| NlaIII | CATG | 3 cut(s) | 239, 261, 464 |
| NlaIV | GGNNCC | 2 cut(s) | 154, 374 |
| NmuCI | GTSAC | 1 cut(s) | 220 |
| NspI | RCATGY | 1 cut(s) | 239 |
| PfeI | GAWTC | 1 cut(s) | 308 |
| PkrI | GCNGC | 3 cut(s) | 405, 489, 531 |
| Psp6I | CCWGG | 2 cut(s) | 375, 543 |
| PspGI | CCWGG | 2 cut(s) | 375, 543 |
| PspN4I | GGNNCC | 2 cut(s) | 154, 374 |
| PspPI | GGNCC | 1 cut(s) | 558 |
| RsaI | GTAC | 4 cut(s) | 26, 132, 415, 502 |
| RsaNI | GTAC | 4 cut(s) | 25, 131, 414, 501 |
| SaqAI | TTAA | 1 cut(s) | 105 |
| SatI | GCNGC | 3 cut(s) | 404, 488, 530 |
| Sau3AI | GATC | 2 cut(s) | 387, 511 |
| Sau96I | GGNCC | 1 cut(s) | 558 |
| ScrFI | CCNGG | 3 cut(s) | 157, 377, 545 |
| SduI | GDGCHC | 1 cut(s) | 375 |
| SetI | ASST | 5 cut(s) | 52, 88, 111, 360, 378 |
| SexAI | ACCWGGT | 1 cut(s) | 375 |
| SfaNI | GCATC | 2 cut(s) | 304, 390 |
| SmlI | CTYRAG | 1 cut(s) | 93 |
| SmoI | CTYRAG | 1 cut(s) | 93 |
| Sse9I | AATT | 3 cut(s) | 269, 336, 363 |
| SsiI | CCGC | 2 cut(s) | 191, 403 |
| SspMI | CTAG | 2 cut(s) | 231, 562 |
| StyD4I | CCNGG | 3 cut(s) | 155, 375, 543 |
| StyI | CCWWGG | 1 cut(s) | 437 |
| TasI | AATT | 3 cut(s) | 269, 336, 363 |
| TatI | WGTACW | 3 cut(s) | 24, 413, 500 |
| TauI | GCSGC | 1 cut(s) | 406 |
| TfiI | GAWTC | 1 cut(s) | 308 |
| Tru1I | TTAA | 1 cut(s) | 105 |
| Tru9I | TTAA | 1 cut(s) | 105 |
| TscAI | CASTG | 1 cut(s) | 39 |
| TseFI | GTSAC | 1 cut(s) | 220 |
| TseI | GCWGC | 2 cut(s) | 487, 529 |
| Tsp45I | GTSAC | 1 cut(s) | 220 |
| TspDTI | ATGAA | 1 cut(s) | 477 |
| TspRI | CASTG | 1 cut(s) | 39 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 93 |
| XceI | RCATGY | 1 cut(s) | 239 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 142 |
| XspI | CTAG | 2 cut(s) | 231, 562 |