Rroxscaffold_7G00173600

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000072
Physical Location & Seq
Reverse (-)
13571817 .. 13574625
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173600.1

Sequence Viewer

Length: 594 bp
ATGATTATGCATGATCATGTTGTACGTGAAGAAATCCAAACACATATATCTGAGGAGACTAAAAAAGGAGTCATGTCTGCCTTATTTTCACAAAGGCCATCTAAATTTGTTTTTCAAAGGATTCAATCAATGCACAAAGCAAGAGACAAACACAAACCTCTAAGCTATATCATGTGCATGGGAAACAGCCTTTGGATGCAGCTGCCTGAGAAAGTGGGGTGTTGGTGGGATGTTTATATAAGAGCCTGTGAGCCGGAGGGGAGTCAGGACAAACCATTTCATCATGGTGGGTCTTCACATGGGCAACAAGAGTTTTCTTGGAGGCGTCCTAGGCCGGGAACAATTAAACTAAATATTGATGGAGCACTGAAGATGGACCGGCGTGTATTTGGGACTGGCGCAGTTTGCAGAGATGAGGATGGGGCCTGCGTTGGTGTTCTTGCTGTACCAGGAAGAGGATTTTTGAGCCCGCAAACTTGTGAGTTTTTAGCCTTAATCAATGGGTTACATTTTTGTCTTCAAGCGGGGTTTTCACACCTGGAGATTGACGGTGATGCCCAGAACATATTCACAGCTCTTAACTCATCACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

197

Amino Acids

22.2

Weight (kDa)

7.68

Isoelectric Point (pI)

65.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 118 - 196 3.6e-14 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000072)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38920
fragaria_vesca FvH4_1g15990 FvH4_1g20960 FvH4_1g24221 FvH4_1g24420 FvH4_2g01203 FvH4_2g08721 FvH4_2g09751 FvH4_2g13111 FvH4_2g14361 FvH4_2g19531 FvH4_2g20801 FvH4_2g36230 FvH4_3g07262 FvH4_3g14231 FvH4_3g21341 FvH4_3g23591 FvH4_3g27972 FvH4_3g28692 FvH4_4g05541 FvH4_4g07351 FvH4_4g07410 FvH4_4g13062 FvH4_4g15487 FvH4_4g15790 FvH4_4g17671 FvH4_4g26583 FvH4_4g28900 FvH4_4g31671 FvH4_5g25821 FvH4_5g31851 FvH4_5g38981 FvH4_6g29902 FvH4_7g03001 FvH4_7g10254
malus_domestica MD03G1147200.v1.1 MD15G1309200.v1.1 MD17G1150900.v1.1
prunus_persica Prupe.1G205500_v2.0.a1 Prupe.2G069500_v2.0.a1 Prupe.2G082600_v2.0.a1 Prupe.3G063400_v2.0.a1 Prupe.3G126800_v2.0.a1 Prupe.3G133900_v2.0.a1 Prupe.3G283300_v2.0.a1 Prupe.4G143500_v2.0.a1 Prupe.4G186900_v2.0.a1 Prupe.6G204400_v2.0.a1 Prupe.6G272800_v2.0.a1 Prupe.7G035500_v2.0.a1 Prupe.7G038400_v2.0.a1 Prupe.7G049300_v2.0.a1 Prupe.7G076800_v2.0.a1 Prupe.8G099500_v2.0.a1 Prupe.8G101300_v2.0.a1
pyrus_communis pycom03g10120 pycom04g16660 pycom08g19920 pycom11g21950 pycom14g01510 pycom14g06470
rosa_chinensis RchiOBHm_Chr1g0323941 RchiOBHm_Chr1g0325331 RchiOBHm_Chr1g0339311 RchiOBHm_Chr1g0345991 RchiOBHm_Chr1g0354891 RchiOBHm_Chr2g0118041 RchiOBHm_Chr3g0468731 RchiOBHm_Chr3g0488841 RchiOBHm_Chr4g0388981 RchiOBHm_Chr4g0398671 RchiOBHm_Chr4g0400431 RchiOBHm_Chr4g0402201 RchiOBHm_Chr4g0430711 RchiOBHm_Chr4g0432871 RchiOBHm_Chr4g0437761 RchiOBHm_Chr5g0066251 RchiOBHm_Chr5g0078071 RchiOBHm_Chr6g0279961 RchiOBHm_Chr6g0289791 RchiOBHm_Chr7g0187741 RchiOBHm_Chr7g0208481
rosa_laevigata RLG00000002376 RLG00000011026 RLG00000011403 RLG00000012646 RLG00000014435 RLG00000014869 RLG00000015450 RLG00000015464 RLG00000016451 RLG00000017121 RLG00000018788 RLG00000018819 RLG00000021054 RLG00000028424 RLG00000030071 RLG00000031242 RLG00000031840 RLG00000031955 RLG00000033437 RLG00000033571 RLG00000034722 RLG00000035706 RLG00000035774 RLG00000036341 RLG00000036639
rosa_multiflora Rmu_co8023178.1_g000001 Rmu_co8119834.1_g000001 Rmu_co8158796.1_g000001 Rmu_co8339863.1_g000001 Rmu_co8512857.1_g000001 Rmu_sc0000174.1_g000006 Rmu_sc0000206.1_g000002 Rmu_sc0000266.1_g000002 Rmu_sc0000361.1_g000026 Rmu_sc0000493.1_g000035 Rmu_sc0000600.1_g000057 Rmu_sc0000796.1_g000012 Rmu_sc0000882.1_g000045 Rmu_sc0000913.1_g000014 Rmu_sc0000946.1_g000041 Rmu_sc0000966.1_g000013 Rmu_sc0001018.1_g000024 Rmu_sc0001018.1_g000025 Rmu_sc0001159.1_g000043 Rmu_sc0001287.1_g000024 Rmu_sc0001291.1_g000041 Rmu_sc0001380.1_g000028 Rmu_sc0001767.1_g000008 Rmu_sc0001802.1_g000010 Rmu_sc0001824.1_g000009 Rmu_sc0001850.1_g000024 Rmu_sc0002105.1_g000011 Rmu_sc0002129.1_g000032 Rmu_sc0002845.1_g000002 Rmu_sc0003054.1_g000001 Rmu_sc0003063.1_g000035 Rmu_sc0003131.1_g000034 Rmu_sc0003303.1_g000022 Rmu_sc0004347.1_g000013 Rmu_sc0004521.1_g000015 Rmu_sc0004618.1_g000056 Rmu_sc0004757.1_g000013 Rmu_sc0005056.1_g000003 Rmu_sc0005354.1_g000011 Rmu_sc0005385.1_g000018 Rmu_sc0005928.1_g000007 Rmu_sc0005933.1_g000003 Rmu_sc0006223.1_g000044 Rmu_sc0006314.1_g000007 Rmu_sc0006399.1_g000008 Rmu_sc0006847.1_g000001 Rmu_sc0007698.1_g000004 Rmu_sc0008509.1_g000023 Rmu_sc0008804.1_g000010 Rmu_sc0008818.1_g000001 Rmu_sc0009489.1_g000001 Rmu_sc0010407.1_g000001 Rmu_sc0010532.1_g000006 Rmu_sc0010988.1_g000001 Rmu_sc0012343.1_g000003 Rmu_sc0012922.1_g000001 Rmu_sc0013078.1_g000009 Rmu_sc0013419.1_g000005 Rmu_sc0013453.1_g000002 Rmu_sc0017867.1_g000004 Rmu_sc0023349.1_g000001 Rmu_sc0025859.1_g000001 Rmu_sc0028153.1_g000002 Rmu_sc0029528.1_g000001 Rmu_sc0030464.1_g000001 Rmu_sc0035933.1_g000001 Rmu_sc0038973.1_g000001 Rmu_sc0040007.1_g000003 Rmu_ssc0000053.1_g000007 Rmu_ssc0000128.1_g000001
rosa_roxburghii Rroxscaffold_175G00431900 Rroxscaffold_1G00018370 Rroxscaffold_1G00035430 Rroxscaffold_1G00040660 Rroxscaffold_1G00048390 Rroxscaffold_1G00048520 Rroxscaffold_2G00078960 Rroxscaffold_2G00086820 Rroxscaffold_2G00112940 Rroxscaffold_2G00118350 Rroxscaffold_2G00139200 Rroxscaffold_2G00141770 Rroxscaffold_3G00221980 Rroxscaffold_3G00227730 Rroxscaffold_3G00241010 Rroxscaffold_3G00242660 Rroxscaffold_4G00305460 Rroxscaffold_4G00324810 Rroxscaffold_4G00326090 Rroxscaffold_5G00334080 Rroxscaffold_5G00335320 Rroxscaffold_5G00344260 Rroxscaffold_5G00348290 Rroxscaffold_5G00381300 Rroxscaffold_6G00392050 Rroxscaffold_6G00401420 Rroxscaffold_6G00405930 Rroxscaffold_6G00418270 Rroxscaffold_6G00421490 Rroxscaffold_7G00167580 Rroxscaffold_7G00172500 Rroxscaffold_7G00173600 Rroxscaffold_7G00185890 Rroxscaffold_7G00192320 Rroxscaffold_7G00197110 Rroxscaffold_7G00209660 Rroxscaffold_7G00211150 Rroxscaffold_7G00216650
rosa_rugosa Rorug01G0040600 Rorug01G0063600 Rorug01G0071700 Rorug01G0184500 Rorug01G0302600 Rorug01G0322600 Rorug01G0403900 Rorug02G0026600 Rorug02G0026700 Rorug02G0074300 Rorug02G0074400 Rorug02G0074500.1 Rorug02G0326900 Rorug02G0351800 Rorug02G0378400 Rorug02G0473800 Rorug03G0023400 Rorug03G0073100 Rorug03G0074900 Rorug03G0118200 Rorug03G0129800 Rorug03G0150100 Rorug03G0178300 Rorug03G0192600 Rorug03G0201700 Rorug03G0231800 Rorug03G0271300 Rorug03G0360000.1 Rorug04G0026900 Rorug04G0036300 Rorug04G0081200 Rorug04G0129600 Rorug04G0153600 Rorug04G0169000 Rorug04G0211000 Rorug04G0211000 Rorug04G0440300 Rorug05G0008100 Rorug05G0083200 Rorug05G0137000 Rorug05G0251200 Rorug05G0411900 Rorug05G0422200 Rorug05G0472700 Rorug06G0026700 Rorug06G0062900 Rorug06G0063000 Rorug06G0069800 Rorug06G0081100 Rorug06G0093500 Rorug06G0095300 Rorug06G0241900 Rorug06G0480400 Rorug07G0043700 Rorug07G0113100 Rorug07G0211300.1 Rorug07G0267700 Rorug07G0281900 Rorug07G0307100
rosa_samantha Rh1AG057800 Rh1AG058600 Rh1AG167200 Rh1AG312300 Rh1AG364500 Rh1BG048900 Rh1BG120100 Rh1BG275500 Rh1BG351000 Rh1CG060500 Rh1CG060600 Rh1DG064500 Rh2AG116500 Rh2BG281500 Rh2BG454900 Rh2BG458000 Rh2BG661700 Rh2CG270000 Rh2DG031300 Rh2DG465000 Rh3DG087300 Rh4AG000500 Rh4AG015500 Rh4AG212800 Rh4BG073500 Rh4BG077400 Rh4BG096800 Rh4BG390300 Rh4CG238100 Rh4CG325900 Rh5AG150200 Rh5CG253600 Rh5DG148600 Rh6AG397200 Rh6BG405900 Rh7BG108000 Rh7BG438000 Rh7CG128200 Rh7CG286000 Rh7CG372000 Rh7CG454900
rosa_wichuraiana Rw1G016000 Rw2G002480 Rw3G029030 Rw4G012010 Rw4G019280 Rw4G020280 Rw4G026190 Rw7G009640 Rw7G020950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 470, 524
AcsI RAATTY 1 cut(s) 104
AcuI CTGAAG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 325
AfaI GTAC 2 cut(s) 24, 447
AfiI CCNNNNNNNGG 2 cut(s) 335, 455
AgsI TTSAA 3 cut(s) 116, 125, 521
AjnI CCWGG 2 cut(s) 448, 537
AjuI GAANNNNNNNTTGG 2 cut(s) 175, 207
AluBI AGCT 3 cut(s) 165, 202, 575
AluI AGCT 3 cut(s) 165, 202, 575
Alw21I GWGCWC 1 cut(s) 367
Alw26I GTCTC 2 cut(s) 50, 138
AoxI GGCC 3 cut(s) 95, 332, 423
ApeKI GCWGC 2 cut(s) 199, 202
ApoI RAATTY 1 cut(s) 104
Asp700I GAANNNNTTC 1 cut(s) 566
AspA2I CCTAGG 1 cut(s) 329
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 2 cut(s) 376, 423
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 1 cut(s) 563
AvaII GGWCC 1 cut(s) 376
AvrII CCTAGG 1 cut(s) 329
BanII GRGCYC 1 cut(s) 470
BbsI GAAGAC 2 cut(s) 285, 509
Bbv12I GWGCWC 1 cut(s) 367
BbvI GCAGC 2 cut(s) 189, 211
BccI CCATC 4 cut(s) 106, 353, 367, 413
BciT130I CCWGG 2 cut(s) 450, 539
BclI TGATCA 1 cut(s) 13
BcnI CCSGG 1 cut(s) 336
BcoDI GTCTC 2 cut(s) 50, 138
BfaI CTAG 1 cut(s) 330
BisI GCNGC 2 cut(s) 200, 203
BlnI CCTAGG 1 cut(s) 329
BlsI GCNGC 2 cut(s) 201, 204
Bme1390I CCNGG 3 cut(s) 336, 450, 539
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 2 cut(s) 376, 423
BmiI GGNNCC 1 cut(s) 424
BmrFI CCNGG 3 cut(s) 336, 450, 539
BmsI GCATC 2 cut(s) 186, 544
BpiI GAAGAC 2 cut(s) 285, 509
BpmI CTGGAG 1 cut(s) 560
BpuMI CCSGG 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 325
BsaJI CCNNGG 1 cut(s) 329
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 455
Bse118I RCCGGY 1 cut(s) 378
Bse1I ACTGG 1 cut(s) 400
BseBI CCWGG 2 cut(s) 450, 539
BseDI CCNNGG 1 cut(s) 329
BseGI GGATG 3 cut(s) 201, 235, 424
BseLI CCNNNNNNNGG 2 cut(s) 335, 455
BseMII CTCAG 2 cut(s) 42, 198
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 2 cut(s) 189, 211
BshFI GGCC 3 cut(s) 97, 334, 425
BsiHKAI GWGCWC 1 cut(s) 367
BsiSI CCGG 3 cut(s) 254, 335, 379
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 2 cut(s) 335, 455
BsmAI GTCTC 2 cut(s) 50, 138
BsmFI GGGAC 1 cut(s) 406
BsnI GGCC 3 cut(s) 97, 334, 425
Bsp1286I GDGCHC 2 cut(s) 367, 470
Bsp143I GATC 1 cut(s) 13
BspACI CCGC 2 cut(s) 470, 524
BspANI GGCC 3 cut(s) 97, 334, 425
BspCNI CTCAG 2 cut(s) 43, 199
BspLI GGNNCC 1 cut(s) 424
BsrFI RCCGGY 1 cut(s) 378
BsrI ACTGG 1 cut(s) 400
BssAI RCCGGY 1 cut(s) 378
BssECI CCNNGG 1 cut(s) 329
BssMI GATC 1 cut(s) 13
BssNI GRCGYC 1 cut(s) 325
BssT1I CCWWGG 1 cut(s) 329
Bst2UI CCWGG 2 cut(s) 450, 539
Bst4CI ACNGT 2 cut(s) 551, 591
Bst6I CTCTTC 1 cut(s) 448
BstACI GRCGYC 1 cut(s) 325
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 427, 470
BstDEI CTNAG 3 cut(s) 51, 161, 207
BstF5I GGATG 3 cut(s) 201, 235, 424
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 1 cut(s) 16
BstMAI GTCTC 2 cut(s) 50, 138
BstMBI GATC 1 cut(s) 13
BstMWI GCNNNNNNNGC 2 cut(s) 331, 405
BstNI CCWGG 2 cut(s) 450, 539
BstSCI CCNGG 3 cut(s) 334, 448, 537
BstV1I GCAGC 2 cut(s) 189, 211
BstV2I GAAGAC 2 cut(s) 285, 509
BsuRI GGCC 3 cut(s) 97, 334, 425
BtsCI GGATG 3 cut(s) 201, 235, 424
BtsIMutI CAGTG 1 cut(s) 365
Cac8I GCNNGC 2 cut(s) 427, 470
CfoI GCGC 1 cut(s) 401
Cfr10I RCCGGY 1 cut(s) 378
Cfr13I GGNCC 2 cut(s) 376, 423
CseI GACGC 1 cut(s) 314
Csp6I GTAC 2 cut(s) 23, 446
CviAII CATG 7 cut(s) 11, 17, 73, 172, 178, 284, 299
CviQI GTAC 2 cut(s) 23, 446
DdeI CTNAG 3 cut(s) 51, 161, 207
DpnI GATC 1 cut(s) 15
DpnII GATC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 448
EarI CTCTTC 1 cut(s) 448
Eco130I CCWWGG 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 470
Eco47I GGWCC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 389
EcoO109I RGGNCCY 1 cut(s) 423
EcoRII CCWGG 2 cut(s) 448, 537
EcoT14I CCWWGG 1 cut(s) 329
EcoT22I ATGCAT 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 470
ErhI CCWWGG 1 cut(s) 329
FaeI CATG 7 cut(s) 14, 20, 76, 175, 181, 287, 302
FaqI GGGAC 1 cut(s) 406
FatI CATG 7 cut(s) 10, 16, 72, 171, 177, 283, 298
FauI CCCGC 2 cut(s) 477, 517
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 2 cut(s) 200, 203
FokI GGATG 3 cut(s) 208, 242, 431
FriOI GRGCYC 1 cut(s) 470
Fsp4HI GCNGC 2 cut(s) 200, 203
FspBI CTAG 1 cut(s) 330
GlaI GCGC 1 cut(s) 400
GluI GCNGC 2 cut(s) 200, 203
GsuI CTGGAG 1 cut(s) 560
HaeIII GGCC 3 cut(s) 97, 334, 425
HapII CCGG 3 cut(s) 254, 335, 379
HgaI GACGC 1 cut(s) 314
HhaI GCGC 1 cut(s) 401
Hin1I GRCGYC 1 cut(s) 325
Hin1II CATG 7 cut(s) 14, 20, 76, 175, 181, 287, 302
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HinfI GANTC 3 cut(s) 69, 121, 262
HpaII CCGG 3 cut(s) 254, 335, 379
HphI GGTGA 1 cut(s) 563
Hpy188I TCNGA 1 cut(s) 52
Hpy188III TCNNGA 1 cut(s) 266
HpyCH4III ACNGT 2 cut(s) 551, 591
HpyCH4IV ACGT 1 cut(s) 25
HpyCH4V TGCA 5 cut(s) 10, 133, 177, 199, 408
HpyF10VI GCNNNNNNNGC 2 cut(s) 331, 405
HpyF3I CTNAG 3 cut(s) 51, 161, 207
HpySE526I ACGT 1 cut(s) 25
Hsp92I GRCGYC 1 cut(s) 325
Hsp92II CATG 7 cut(s) 14, 20, 76, 175, 181, 287, 302
HspAI GCGC 1 cut(s) 399
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 1 cut(s) 13
LmnI GCTCC 1 cut(s) 362
Lsp1109I GCAGC 2 cut(s) 189, 211
LweI GCATC 2 cut(s) 186, 544
MaeI CTAG 1 cut(s) 330
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 1 cut(s) 504
MalI GATC 1 cut(s) 15
MboI GATC 1 cut(s) 13
MboII GAAGA 5 cut(s) 41, 285, 382, 465, 509
MhlI GDGCHC 2 cut(s) 367, 470
MluCI AATT 2 cut(s) 104, 342
MlyI GAGTC 2 cut(s) 78, 271
MnlI CCTC 6 cut(s) 46, 168, 250, 315, 409, 449
Mph1103I ATGCAT 1 cut(s) 12
MroXI GAANNNNTTC 1 cut(s) 566
MseI TTAA 3 cut(s) 345, 494, 579
MslI CAYNNNNRTG 3 cut(s) 15, 176, 285
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 3 cut(s) 254, 335, 379
MspR9I CCNGG 3 cut(s) 336, 450, 539
MvaI CCWGG 2 cut(s) 450, 539
MwoI GCNNNNNNNGC 2 cut(s) 331, 405
NciI CCSGG 1 cut(s) 336
NdeII GATC 1 cut(s) 13
NlaIII CATG 7 cut(s) 14, 20, 76, 175, 181, 287, 302
NlaIV GGNNCC 1 cut(s) 424
NsiI ATGCAT 1 cut(s) 12
PdmI GAANNNNTTC 1 cut(s) 566
PfeI GAWTC 1 cut(s) 121
PkrI GCNGC 2 cut(s) 201, 204
PleI GAGTC 2 cut(s) 77, 270
PpsI GAGTC 2 cut(s) 77, 270
Ppu21I YACGTR 1 cut(s) 26
Psp6I CCWGG 2 cut(s) 448, 537
PspGI CCWGG 2 cut(s) 448, 537
PspN4I GGNNCC 1 cut(s) 424
PspPI GGNCC 2 cut(s) 376, 423
PvuII CAGCTG 1 cut(s) 202
RsaI GTAC 2 cut(s) 24, 447
RsaNI GTAC 2 cut(s) 23, 446
RseI CAYNNNNRTG 3 cut(s) 15, 176, 285
SaqAI TTAA 3 cut(s) 345, 494, 579
SatI GCNGC 2 cut(s) 200, 203
Sau3AI GATC 1 cut(s) 13
Sau96I GGNCC 2 cut(s) 376, 423
SchI GAGTC 2 cut(s) 78, 271
ScrFI CCNGG 3 cut(s) 336, 450, 539
SduI GDGCHC 2 cut(s) 367, 470
SetI ASST 6 cut(s) 28, 160, 167, 204, 540, 577
SfaNI GCATC 2 cut(s) 186, 544
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 3 cut(s) 15, 176, 285
Sse9I AATT 2 cut(s) 104, 342
SsiI CCGC 2 cut(s) 470, 524
SspI AATATT 1 cut(s) 355
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 3 cut(s) 334, 448, 537
StyI CCWWGG 1 cut(s) 329
TaaI ACNGT 2 cut(s) 551, 591
TaiI ACGT 1 cut(s) 28
TasI AATT 2 cut(s) 104, 342
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 3 cut(s) 345, 494, 579
Tru9I TTAA 3 cut(s) 345, 494, 579
TscAI CASTG 1 cut(s) 372
TseI GCWGC 2 cut(s) 199, 202
TspDTI ATGAA 1 cut(s) 269
TspRI CASTG 1 cut(s) 372
VpaK11BI GGWCC 1 cut(s) 376
XapI RAATTY 1 cut(s) 104
XmaJI CCTAGG 1 cut(s) 329
XmnI GAANNNNTTC 1 cut(s) 566
XspI CTAG 1 cut(s) 330
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.