ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 141 |
| AccB1I | GGYRCC | 2 cut(s) | 28, 63 |
| AccBSI | CCGCTC | 1 cut(s) | 162 |
| AccII | CGCG | 1 cut(s) | 315 |
| AccIII | TCCGGA | 1 cut(s) | 15 |
| AciI | CCGC | 4 cut(s) | 90, 160, 174, 184 |
| AclWI | GGATC | 2 cut(s) | 93, 175 |
| AcoI | YGGCCR | 1 cut(s) | 295 |
| AcuI | CTGAAG | 1 cut(s) | 67 |
| AcyI | GRCGYC | 1 cut(s) | 138 |
| AgsI | TTSAA | 1 cut(s) | 235 |
| AjnI | CCWGG | 1 cut(s) | 348 |
| Alw26I | GTCTC | 1 cut(s) | 49 |
| AlwI | GGATC | 2 cut(s) | 93, 175 |
| Aor13HI | TCCGGA | 1 cut(s) | 15 |
| AoxI | GGCC | 3 cut(s) | 87, 295, 354 |
| ApeKI | GCWGC | 1 cut(s) | 226 |
| AspLEI | GCGC | 1 cut(s) | 341 |
| AspS9I | GGNCC | 3 cut(s) | 87, 346, 354 |
| AsuC2I | CCSGG | 1 cut(s) | 61 |
| AsuHPI | GGTGA | 2 cut(s) | 146, 193 |
| AvaII | GGWCC | 1 cut(s) | 346 |
| BalI | TGGCCA | 1 cut(s) | 297 |
| BanI | GGYRCC | 2 cut(s) | 28, 63 |
| BbsI | GAAGAC | 1 cut(s) | 64 |
| BbvI | GCAGC | 1 cut(s) | 213 |
| BccI | CCATC | 2 cut(s) | 110, 157 |
| BciT130I | CCWGG | 1 cut(s) | 350 |
| BciVI | GTATCC | 1 cut(s) | 325 |
| BcnI | CCSGG | 1 cut(s) | 61 |
| BcoDI | GTCTC | 1 cut(s) | 49 |
| BfmI | CTRYAG | 2 cut(s) | 94, 108 |
| BfuI | GTATCC | 1 cut(s) | 325 |
| BisI | GCNGC | 3 cut(s) | 90, 174, 227 |
| BlsI | GCNGC | 3 cut(s) | 91, 175, 228 |
| Bme1390I | CCNGG | 2 cut(s) | 61, 350 |
| Bme18I | GGWCC | 1 cut(s) | 346 |
| BmgT120I | GGNCC | 3 cut(s) | 87, 346, 354 |
| BmiI | GGNNCC | 3 cut(s) | 30, 65, 355 |
| BmrFI | CCNGG | 2 cut(s) | 61, 350 |
| BpiI | GAAGAC | 1 cut(s) | 64 |
| BpuMI | CCSGG | 1 cut(s) | 61 |
| BsaHI | GRCGYC | 1 cut(s) | 138 |
| BsaI | GGTCTC | 1 cut(s) | 49 |
| BsaJI | CCNNGG | 2 cut(s) | 210, 349 |
| BsaWI | WCCGGW | 1 cut(s) | 15 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 146, 176 |
| Bse1I | ACTGG | 1 cut(s) | 298 |
| Bse3DI | GCAATG | 1 cut(s) | 246 |
| BseAI | TCCGGA | 1 cut(s) | 15 |
| BseBI | CCWGG | 1 cut(s) | 350 |
| BseDI | CCNNGG | 2 cut(s) | 210, 349 |
| BseGI | GGATG | 1 cut(s) | 102 |
| BseMI | GCAATG | 1 cut(s) | 246 |
| BseMII | CTCAG | 1 cut(s) | 255 |
| BseNI | ACTGG | 1 cut(s) | 298 |
| BseXI | GCAGC | 1 cut(s) | 213 |
| Bsh1236I | CGCG | 1 cut(s) | 315 |
| BshFI | GGCC | 3 cut(s) | 89, 297, 356 |
| BshNI | GGYRCC | 2 cut(s) | 28, 63 |
| BsiSI | CCGG | 2 cut(s) | 16, 61 |
| BsmAI | GTCTC | 1 cut(s) | 49 |
| BsnI | GGCC | 3 cut(s) | 89, 297, 356 |
| Bso31I | GGTCTC | 1 cut(s) | 49 |
| Bsp13I | TCCGGA | 1 cut(s) | 15 |
| Bsp143I | GATC | 2 cut(s) | 98, 180 |
| Bsp68I | TCGCGA | 1 cut(s) | 315 |
| BspACI | CCGC | 4 cut(s) | 90, 160, 174, 184 |
| BspANI | GGCC | 3 cut(s) | 89, 297, 356 |
| BspCNI | CTCAG | 1 cut(s) | 256 |
| BspEI | TCCGGA | 1 cut(s) | 15 |
| BspFNI | CGCG | 1 cut(s) | 315 |
| BspLI | GGNNCC | 3 cut(s) | 30, 65, 355 |
| BspMAI | CTGCAG | 1 cut(s) | 98 |
| BspPI | GGATC | 2 cut(s) | 93, 175 |
| BspQI | GCTCTTC | 1 cut(s) | 236 |
| BspT107I | GGYRCC | 2 cut(s) | 28, 63 |
| BspTNI | GGTCTC | 1 cut(s) | 49 |
| BsrBI | CCGCTC | 1 cut(s) | 162 |
| BsrDI | GCAATG | 1 cut(s) | 246 |
| BsrI | ACTGG | 1 cut(s) | 298 |
| BssECI | CCNNGG | 2 cut(s) | 210, 349 |
| BssMI | GATC | 2 cut(s) | 98, 180 |
| BssNI | GRCGYC | 1 cut(s) | 138 |
| BssT1I | CCWWGG | 1 cut(s) | 210 |
| Bst2UI | CCWGG | 1 cut(s) | 350 |
| Bst4CI | ACNGT | 1 cut(s) | 112 |
| Bst6I | CTCTTC | 2 cut(s) | 236, 240 |
| BstACI | GRCGYC | 1 cut(s) | 138 |
| BstC8I | GCNNGC | 4 cut(s) | 94, 188, 192, 270 |
| BstDEI | CTNAG | 1 cut(s) | 264 |
| BstF5I | GGATG | 1 cut(s) | 102 |
| BstFNI | CGCG | 1 cut(s) | 315 |
| BstHHI | GCGC | 1 cut(s) | 341 |
| BstKTI | GATC | 2 cut(s) | 101, 183 |
| BstMAI | GTCTC | 1 cut(s) | 49 |
| BstMBI | GATC | 2 cut(s) | 98, 180 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 274 |
| BstNI | CCWGG | 1 cut(s) | 350 |
| BstNSI | RCATGY | 1 cut(s) | 272 |
| BstSCI | CCNGG | 2 cut(s) | 59, 348 |
| BstSFI | CTRYAG | 2 cut(s) | 94, 108 |
| BstUI | CGCG | 1 cut(s) | 315 |
| BstV1I | GCAGC | 1 cut(s) | 213 |
| BstV2I | GAAGAC | 1 cut(s) | 64 |
| BstX2I | RGATCY | 2 cut(s) | 98, 180 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 293 |
| BstYI | RGATCY | 2 cut(s) | 98, 180 |
| BsuI | GTATCC | 1 cut(s) | 325 |
| BsuRI | GGCC | 3 cut(s) | 89, 297, 356 |
| BtsCI | GGATG | 1 cut(s) | 102 |
| BtsIMutI | CAGTG | 1 cut(s) | 261 |
| BtuMI | TCGCGA | 1 cut(s) | 315 |
| Cac8I | GCNNGC | 4 cut(s) | 94, 188, 192, 270 |
| CfoI | GCGC | 1 cut(s) | 341 |
| Cfr13I | GGNCC | 3 cut(s) | 87, 346, 354 |
| CviAII | CATG | 3 cut(s) | 115, 269, 325 |
| CviJI | RGCY | 4 cut(s) | 59, 89, 297, 356 |
| CviKI_1 | RGCY | 4 cut(s) | 59, 89, 297, 356 |
| DdeI | CTNAG | 1 cut(s) | 264 |
| DpnI | GATC | 2 cut(s) | 100, 182 |
| DpnII | GATC | 2 cut(s) | 98, 180 |
| EaeI | YGGCCR | 1 cut(s) | 295 |
| Eam1104I | CTCTTC | 2 cut(s) | 236, 240 |
| EarI | CTCTTC | 2 cut(s) | 236, 240 |
| Eco130I | CCWWGG | 1 cut(s) | 210 |
| Eco31I | GGTCTC | 1 cut(s) | 49 |
| Eco47I | GGWCC | 1 cut(s) | 346 |
| Eco57I | CTGAAG | 1 cut(s) | 67 |
| EcoO109I | RGGNCCY | 1 cut(s) | 354 |
| EcoRII | CCWGG | 1 cut(s) | 348 |
| EcoT14I | CCWWGG | 1 cut(s) | 210 |
| ErhI | CCWWGG | 1 cut(s) | 210 |
| FaeI | CATG | 3 cut(s) | 118, 272, 328 |
| FaiI | YATR | 5 cut(s) | 116, 224, 270, 303, 326 |
| FatI | CATG | 3 cut(s) | 114, 268, 324 |
| Fnu4HI | GCNGC | 3 cut(s) | 90, 174, 227 |
| FokI | GGATG | 1 cut(s) | 89 |
| Fsp4HI | GCNGC | 3 cut(s) | 90, 174, 227 |
| GlaI | GCGC | 1 cut(s) | 340 |
| GluI | GCNGC | 3 cut(s) | 90, 174, 227 |
| HaeIII | GGCC | 3 cut(s) | 89, 297, 356 |
| HapII | CCGG | 2 cut(s) | 16, 61 |
| HhaI | GCGC | 1 cut(s) | 341 |
| Hin1I | GRCGYC | 1 cut(s) | 138 |
| Hin1II | CATG | 3 cut(s) | 118, 272, 328 |
| Hin6I | GCGC | 1 cut(s) | 339 |
| HinP1I | GCGC | 1 cut(s) | 339 |
| HinfI | GANTC | 2 cut(s) | 69, 321 |
| HpaII | CCGG | 2 cut(s) | 16, 61 |
| HphI | GGTGA | 2 cut(s) | 146, 193 |
| Hpy166II | GTNNAC | 1 cut(s) | 9 |
| Hpy188III | TCNNGA | 4 cut(s) | 16, 50, 134, 314 |
| Hpy8I | GTNNAC | 1 cut(s) | 9 |
| Hpy99I | CGWCG | 6 cut(s) | 56, 140, 143, 146, 170, 173 |
| HpyAV | CCTTC | 3 cut(s) | 19, 140, 311 |
| HpyCH4III | ACNGT | 1 cut(s) | 112 |
| HpyCH4IV | ACGT | 1 cut(s) | 138 |
| HpyCH4V | TGCA | 2 cut(s) | 96, 328 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 274 |
| HpyF3I | CTNAG | 1 cut(s) | 264 |
| HpySE526I | ACGT | 1 cut(s) | 138 |
| Hsp92I | GRCGYC | 1 cut(s) | 138 |
| Hsp92II | CATG | 3 cut(s) | 118, 272, 328 |
| HspAI | GCGC | 1 cut(s) | 339 |
| Kpn2I | TCCGGA | 1 cut(s) | 15 |
| Kzo9I | GATC | 2 cut(s) | 98, 180 |
| LguI | GCTCTTC | 1 cut(s) | 236 |
| LmnI | GCTCC | 2 cut(s) | 18, 167 |
| LpnPI | CCDG | 9 cut(s) | 29, 70, 74, 106, 311, 314, 321, 335, 362 |
| Lsp1109I | GCAGC | 1 cut(s) | 213 |
| MaeII | ACGT | 1 cut(s) | 138 |
| MaeIII | GTNAC | 1 cut(s) | 199 |
| MalI | GATC | 2 cut(s) | 100, 182 |
| MbiI | CCGCTC | 1 cut(s) | 162 |
| MboI | GATC | 2 cut(s) | 98, 180 |
| MboII | GAAGA | 4 cut(s) | 64, 73, 223, 257 |
| MflI | RGATCY | 2 cut(s) | 98, 180 |
| MlsI | TGGCCA | 1 cut(s) | 297 |
| MluCI | AATT | 2 cut(s) | 361, 388 |
| MluNI | TGGCCA | 1 cut(s) | 297 |
| MlyI | GAGTC | 2 cut(s) | 78, 315 |
| MnlI | CCTC | 3 cut(s) | 167, 205, 367 |
| Mox20I | TGGCCA | 1 cut(s) | 297 |
| MroI | TCCGGA | 1 cut(s) | 15 |
| MscI | TGGCCA | 1 cut(s) | 297 |
| MseI | TTAA | 1 cut(s) | 251 |
| MslI | CAYNNNNRTG | 1 cut(s) | 221 |
| Msp20I | TGGCCA | 1 cut(s) | 297 |
| MspI | CCGG | 2 cut(s) | 16, 61 |
| MspR9I | CCNGG | 2 cut(s) | 61, 350 |
| MvaI | CCWGG | 1 cut(s) | 350 |
| MvnI | CGCG | 1 cut(s) | 315 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 274 |
| NciI | CCSGG | 1 cut(s) | 61 |
| NdeII | GATC | 2 cut(s) | 98, 180 |
| NlaIII | CATG | 3 cut(s) | 118, 272, 328 |
| NlaIV | GGNNCC | 3 cut(s) | 30, 65, 355 |
| NmeAIII | GCCGAG | 1 cut(s) | 202 |
| NmuCI | GTSAC | 1 cut(s) | 199 |
| NruI | TCGCGA | 1 cut(s) | 315 |
| NspI | RCATGY | 1 cut(s) | 272 |
| PaeI | GCATGC | 1 cut(s) | 272 |
| PciSI | GCTCTTC | 1 cut(s) | 236 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 174 |
| PflFI | GACNNNGTC | 1 cut(s) | 140 |
| PkrI | GCNGC | 3 cut(s) | 91, 175, 228 |
| PleI | GAGTC | 2 cut(s) | 77, 315 |
| PpsI | GAGTC | 2 cut(s) | 77, 315 |
| Psp6I | CCWGG | 1 cut(s) | 348 |
| PspGI | CCWGG | 1 cut(s) | 348 |
| PspN4I | GGNNCC | 3 cut(s) | 30, 65, 355 |
| PspPI | GGNCC | 3 cut(s) | 87, 346, 354 |
| PstI | CTGCAG | 1 cut(s) | 98 |
| PsuI | RGATCY | 2 cut(s) | 98, 180 |
| PsyI | GACNNNGTC | 1 cut(s) | 140 |
| RruI | TCGCGA | 1 cut(s) | 315 |
| RseI | CAYNNNNRTG | 1 cut(s) | 221 |
| SapI | GCTCTTC | 1 cut(s) | 236 |
| SaqAI | TTAA | 1 cut(s) | 251 |
| SatI | GCNGC | 3 cut(s) | 90, 174, 227 |
| Sau3AI | GATC | 2 cut(s) | 98, 180 |
| Sau96I | GGNCC | 3 cut(s) | 87, 346, 354 |
| SchI | GAGTC | 2 cut(s) | 78, 315 |
| ScrFI | CCNGG | 2 cut(s) | 61, 350 |
| SetI | ASST | 5 cut(s) | 30, 132, 141, 159, 216 |
| SfcI | CTRYAG | 2 cut(s) | 94, 108 |
| SinI | GGWCC | 1 cut(s) | 346 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 221 |
| SphI | GCATGC | 1 cut(s) | 272 |
| Sse9I | AATT | 2 cut(s) | 361, 388 |
| SsiI | CCGC | 4 cut(s) | 90, 160, 174, 184 |
| StyD4I | CCNGG | 2 cut(s) | 59, 348 |
| StyI | CCWWGG | 1 cut(s) | 210 |
| TaaI | ACNGT | 1 cut(s) | 112 |
| TaiI | ACGT | 1 cut(s) | 141 |
| TaqI | TCGA | 2 cut(s) | 51, 75 |
| TaqII | GACCGA | 1 cut(s) | 31 |
| TasI | AATT | 2 cut(s) | 361, 388 |
| TauI | GCSGC | 2 cut(s) | 92, 176 |
| Tru1I | TTAA | 1 cut(s) | 251 |
| Tru9I | TTAA | 1 cut(s) | 251 |
| TscAI | CASTG | 1 cut(s) | 268 |
| TseFI | GTSAC | 1 cut(s) | 199 |
| TseI | GCWGC | 1 cut(s) | 226 |
| Tsp45I | GTSAC | 1 cut(s) | 199 |
| TspGWI | ACGGA | 2 cut(s) | 154, 187 |
| TspRI | CASTG | 1 cut(s) | 268 |
| Tth111I | GACNNNGTC | 1 cut(s) | 140 |
| VpaK11BI | GGWCC | 1 cut(s) | 346 |
| XceI | RCATGY | 1 cut(s) | 272 |
| ZraI | GACGTC | 1 cut(s) | 139 |