Rroxscaffold_6G00390660

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008046
Physical Location & Seq
Reverse (-)
6165056 .. 6174033
8978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00390660.1

Sequence Viewer

Length: 1017 bp
ATGGCTAATGAGGAGCATTGTTGGGAATGGGCTCGAAAGTATTTGGAGTACAATCTGTGCTCTACTAAAGACGGCGTTTCGTTGGTGTTGGGTGTAGTCAGTGTTGTCAGTTGGGGTGTTGCCGAGCTTCCAACCCAATACTATACGGCAATGGATTCCAAATCTGATCTGACTGAGGTTCCAGGAAAATTGAGAGGAAGCACTAGTGATTTATTTGGGAATCAGGTTGACATTGTTGGCCGGGGTAATGGGTTTGAAACCTCCAGTAGAGGAAATGTTTTGAGTTCACCTATTTCTCTTCCTCCAAGTAAATCTCCCAGAAGAGAATCATATTACATATCAGCAAGATCTCTGTCAAGGAGTCATACTCCTACAGAAGGATCTCATTTAGCACAAAAAATGACTGGTACATCTAATCGTATTCGCAACTCCAATGAAGAGCCCTTGCTGGGTTCACTTGCATCTAGACAATCTGCACCAACAAATGTCAAGACTGTGCTCTGTGTGGTCTCTCTGATGACTCTCTTTGGCACTTCTAATTATCGATCAGCAGACAATAAAGGACATTTGATTGTTGATTCAAATCCAGGAGTAGTGATGCTAGTAGGAAGAAAGCTTTTGCAGGTACATAGTGGGCTGTTGCAAGAAAAGGATGTTAAAGATAGCAGTGTGATTGGACATGTCCTTGGTTGGGGAATGGCAGCTATTTACATTGGTGGAAGGTTTCCTCAGATTTTCCTCAACTTCAAAAGAAGCAATGTTGAGGGCCTTAATCCTTTAATGTTCGTCTTTGCTATTGTTGGAAATGCCACTTATGTAGCAAGCATTGTTGTGAACAGCTTAGAATGGTCGAAAATCAGACCAAATCTAGCGTGGCTAGTGGACGCTGGAGGGTACAAAGGAAATTTTCTAAAAGTCCACTACAAGATGGATGGTGTCAACATAATGCAAATTCTGTGGGTTTATCTTCAGAAAGCTGCACCAGAAGAACATCTATTATTTGCGGCAACTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

338

Amino Acids

37.12

Weight (kDa)

9.11

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQ-loop PF04193 225 - 284 3.3e-16 PQ loop repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 613
AciI CCGC 1 cut(s) 1004
AclWI GGATC 1 cut(s) 388
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 2 cut(s) 904, 951
AcuI CTGAAG 1 cut(s) 953
AfaI GTAC 4 cut(s) 50, 409, 627, 896
AfiI CCNNNNNNNGG 3 cut(s) 377, 449, 691
AflIII ACRYGT 1 cut(s) 679
AgsI TTSAA 3 cut(s) 257, 582, 748
AhlI ACTAGT 1 cut(s) 203
AjnI CCWGG 2 cut(s) 181, 586
AluBI AGCT 5 cut(s) 127, 616, 704, 840, 977
AluI AGCT 5 cut(s) 127, 616, 704, 840, 977
Alw21I GWGCWC 2 cut(s) 62, 501
Alw26I GTCTC 1 cut(s) 514
AlwI GGATC 1 cut(s) 388
AoxI GGCC 2 cut(s) 238, 766
ApeKI GCWGC 2 cut(s) 701, 977
ApoI RAATTY 2 cut(s) 904, 951
AspS9I GGNCC 1 cut(s) 766
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 1 cut(s) 279
BanII GRGCYC 2 cut(s) 34, 444
Bbv12I GWGCWC 2 cut(s) 62, 501
BbvI GCAGC 2 cut(s) 713, 964
BccI CCATC 2 cut(s) 922, 926
BceAI ACGGC 2 cut(s) 88, 162
BciT130I CCWGG 2 cut(s) 183, 588
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 514
BcuI ACTAGT 1 cut(s) 203
BfaI CTAG 5 cut(s) 204, 465, 602, 869, 878
BfmI CTRYAG 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 613
BglII AGATCT 1 cut(s) 347
BisI GCNGC 3 cut(s) 702, 978, 1005
BlsI GCNGC 3 cut(s) 703, 979, 1006
Bme1390I CCNGG 3 cut(s) 183, 242, 588
BmgT120I GGNCC 1 cut(s) 766
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 3 cut(s) 183, 242, 588
BmsI GCATC 2 cut(s) 470, 588
BplI GAGNNNNNCTC 2 cut(s) 352, 384
BpmI CTGGAG 2 cut(s) 247, 909
BpuMI CCSGG 1 cut(s) 242
Bsa29I ATCGAT 1 cut(s) 544
BsaBI GATNNNNATC 1 cut(s) 582
BsaI GGTCTC 1 cut(s) 514
BsaJI CCNNGG 2 cut(s) 241, 685
Bsc4I CCNNNNNNNGG 3 cut(s) 377, 449, 691
Bse1I ACTGG 2 cut(s) 264, 409
Bse3DI GCAATG 2 cut(s) 156, 763
Bse8I GATNNNNATC 1 cut(s) 582
BseBI CCWGG 2 cut(s) 183, 588
BseCI ATCGAT 1 cut(s) 544
BseDI CCNNGG 2 cut(s) 241, 685
BseGI GGATG 2 cut(s) 658, 937
BseJI GATNNNNATC 1 cut(s) 582
BseLI CCNNNNNNNGG 3 cut(s) 377, 449, 691
BseMI GCAATG 2 cut(s) 156, 763
BseMII CTCAG 2 cut(s) 165, 743
BseNI ACTGG 2 cut(s) 264, 409
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 2 cut(s) 713, 964
BseYI CCCAGC 1 cut(s) 448
BsgI GTGCAG 2 cut(s) 459, 963
BshFI GGCC 2 cut(s) 240, 768
BshVI ATCGAT 1 cut(s) 544
BsiHKAI GWGCWC 2 cut(s) 62, 501
BsiSI CCGG 1 cut(s) 241
BslI CCNNNNNNNGG 3 cut(s) 377, 449, 691
BsmAI GTCTC 1 cut(s) 514
BsnI GGCC 2 cut(s) 240, 768
Bso31I GGTCTC 1 cut(s) 514
Bsp1286I GDGCHC 4 cut(s) 34, 62, 444, 501
Bsp143I GATC 4 cut(s) 166, 347, 380, 545
BspACI CCGC 1 cut(s) 1004
BspANI GGCC 2 cut(s) 240, 768
BspCNI CTCAG 2 cut(s) 166, 742
BspDI ATCGAT 1 cut(s) 544
BspLI GGNNCC 1 cut(s) 180
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 1 cut(s) 388
BspQI GCTCTTC 1 cut(s) 432
BspTNI GGTCTC 1 cut(s) 514
BsrDI GCAATG 2 cut(s) 156, 763
BsrI ACTGG 2 cut(s) 264, 409
BssECI CCNNGG 2 cut(s) 241, 685
BssMI GATC 4 cut(s) 166, 347, 380, 545
BssT1I CCWWGG 1 cut(s) 685
Bst2UI CCWGG 2 cut(s) 183, 588
Bst4CI ACNGT 1 cut(s) 496
Bst6I CTCTTC 3 cut(s) 303, 316, 432
BstC8I GCNNGC 1 cut(s) 823
BstDEI CTNAG 3 cut(s) 174, 729, 841
BstENI CCTNNNNNAGG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 658, 937
BstKTI GATC 4 cut(s) 169, 350, 383, 548
BstMAI GTCTC 1 cut(s) 514
BstMBI GATC 4 cut(s) 166, 347, 380, 545
BstNI CCWGG 2 cut(s) 183, 588
BstNSI RCATGY 1 cut(s) 683
BstSCI CCNGG 3 cut(s) 181, 240, 586
BstSFI CTRYAG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 713, 964
BstX2I RGATCY 2 cut(s) 347, 380
BstYI RGATCY 2 cut(s) 347, 380
Bsu15I ATCGAT 1 cut(s) 544
BsuRI GGCC 2 cut(s) 240, 768
BsuTUI ATCGAT 1 cut(s) 544
BtsCI GGATG 2 cut(s) 658, 937
BtsI GCAGTG 1 cut(s) 673
BtsIMutI CAGTG 2 cut(s) 106, 673
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 1 cut(s) 823
Cfr13I GGNCC 1 cut(s) 766
ClaI ATCGAT 1 cut(s) 544
CseI GACGC 1 cut(s) 893
Csp6I GTAC 4 cut(s) 49, 408, 626, 895
CspCI CAANNNNNGTGG 2 cut(s) 938, 973
CviAII CATG 1 cut(s) 680
CviQI GTAC 4 cut(s) 49, 408, 626, 895
DdeI CTNAG 3 cut(s) 174, 729, 841
DpnI GATC 4 cut(s) 168, 349, 382, 547
DpnII GATC 4 cut(s) 166, 347, 380, 545
EaeI YGGCCR 1 cut(s) 238
Eam1104I CTCTTC 3 cut(s) 303, 316, 432
EarI CTCTTC 3 cut(s) 303, 316, 432
Eco130I CCWWGG 1 cut(s) 685
Eco24I GRGCYC 2 cut(s) 34, 444
Eco31I GGTCTC 1 cut(s) 514
Eco57I CTGAAG 1 cut(s) 953
EcoNI CCTNNNNNAGG 1 cut(s) 375
EcoO109I RGGNCCY 1 cut(s) 766
EcoRII CCWGG 2 cut(s) 181, 586
EcoT14I CCWWGG 1 cut(s) 685
EcoT38I GRGCYC 2 cut(s) 34, 444
ErhI CCWWGG 1 cut(s) 685
FaeI CATG 1 cut(s) 683
FaiI YATR 9 cut(s) 144, 331, 338, 366, 630, 681, 816, 944, 1013
FatI CATG 1 cut(s) 679
Fnu4HI GCNGC 3 cut(s) 702, 978, 1005
FokI GGATG 2 cut(s) 665, 944
FriOI GRGCYC 2 cut(s) 34, 444
Fsp4HI GCNGC 3 cut(s) 702, 978, 1005
FspBI CTAG 5 cut(s) 204, 465, 602, 869, 878
GluI GCNGC 3 cut(s) 702, 978, 1005
GsaI CCCAGC 1 cut(s) 452
GsuI CTGGAG 2 cut(s) 247, 909
HaeIII GGCC 2 cut(s) 240, 768
HapII CCGG 1 cut(s) 241
HgaI GACGC 1 cut(s) 893
Hin1II CATG 1 cut(s) 683
HincII GTYRAC 2 cut(s) 229, 940
HindII GTYRAC 2 cut(s) 229, 940
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 6 cut(s) 155, 220, 326, 361, 520, 578
HpaII CCGG 1 cut(s) 241
HphI GGTGA 1 cut(s) 279
Hpy166II GTNNAC 7 cut(s) 229, 287, 455, 835, 883, 919, 940
Hpy188I TCNGA 6 cut(s) 166, 171, 516, 732, 860, 972
Hpy188III TCNNGA 2 cut(s) 465, 490
Hpy8I GTNNAC 7 cut(s) 229, 287, 455, 835, 883, 919, 940
HpyAV CCTTC 2 cut(s) 371, 714
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4V TGCA 6 cut(s) 461, 476, 622, 643, 949, 980
HpyF3I CTNAG 3 cut(s) 174, 729, 841
Hsp92II CATG 1 cut(s) 683
Kzo9I GATC 4 cut(s) 166, 347, 380, 545
LguI GCTCTTC 1 cut(s) 432
LmnI GCTCC 1 cut(s) 13
Lsp1109I GCAGC 2 cut(s) 713, 964
LweI GCATC 2 cut(s) 470, 588
MaeI CTAG 5 cut(s) 204, 465, 602, 869, 878
MalI GATC 4 cut(s) 168, 349, 382, 547
MboI GATC 4 cut(s) 166, 347, 380, 545
MboII GAAGA 6 cut(s) 290, 333, 449, 621, 959, 998
MflI RGATCY 2 cut(s) 347, 380
MhlI GDGCHC 4 cut(s) 34, 62, 444, 501
MluCI AATT 4 cut(s) 188, 538, 904, 951
MlyI GAGTC 2 cut(s) 370, 514
MmeI TCCRAC 2 cut(s) 155, 781
MseI TTAA 3 cut(s) 657, 771, 779
MslI CAYNNNNRTG 1 cut(s) 830
MspI CCGG 1 cut(s) 241
MspR9I CCNGG 3 cut(s) 183, 242, 588
MvaI CCWGG 2 cut(s) 183, 588
NciI CCSGG 1 cut(s) 242
NdeII GATC 4 cut(s) 166, 347, 380, 545
NlaIII CATG 1 cut(s) 683
NlaIV GGNNCC 1 cut(s) 180
NmeAIII GCCGAG 1 cut(s) 148
NspI RCATGY 1 cut(s) 683
PciI ACATGT 1 cut(s) 679
PciSI GCTCTTC 1 cut(s) 432
PfeI GAWTC 4 cut(s) 155, 220, 326, 578
PfoI TCCNGGA 2 cut(s) 181, 586
PkrI GCNGC 3 cut(s) 703, 979, 1006
PleI GAGTC 2 cut(s) 369, 514
PpsI GAGTC 2 cut(s) 369, 514
PscI ACATGT 1 cut(s) 679
Psp6I CCWGG 2 cut(s) 181, 586
PspFI CCCAGC 1 cut(s) 448
PspGI CCWGG 2 cut(s) 181, 586
PspN4I GGNNCC 1 cut(s) 180
PspPI GGNCC 1 cut(s) 766
PsuI RGATCY 2 cut(s) 347, 380
RsaI GTAC 4 cut(s) 50, 409, 627, 896
RsaNI GTAC 4 cut(s) 49, 408, 626, 895
RseI CAYNNNNRTG 1 cut(s) 830
SapI GCTCTTC 1 cut(s) 432
SaqAI TTAA 3 cut(s) 657, 771, 779
SatI GCNGC 3 cut(s) 702, 978, 1005
Sau3AI GATC 4 cut(s) 166, 347, 380, 545
Sau96I GGNCC 1 cut(s) 766
SchI GAGTC 2 cut(s) 370, 514
ScrFI CCNGG 3 cut(s) 183, 242, 588
SduI GDGCHC 4 cut(s) 34, 62, 444, 501
SfaNI GCATC 2 cut(s) 470, 588
SfcI CTRYAG 1 cut(s) 372
SmiMI CAYNNNNRTG 1 cut(s) 830
SpeI ACTAGT 1 cut(s) 203
Sse9I AATT 4 cut(s) 188, 538, 904, 951
SsiI CCGC 1 cut(s) 1004
SspMI CTAG 5 cut(s) 204, 465, 602, 869, 878
StyD4I CCNGG 3 cut(s) 181, 240, 586
StyI CCWWGG 1 cut(s) 685
TaaI ACNGT 1 cut(s) 496
TaqI TCGA 3 cut(s) 34, 544, 851
TasI AATT 4 cut(s) 188, 538, 904, 951
TatI WGTACW 1 cut(s) 48
TauI GCSGC 1 cut(s) 1007
TfiI GAWTC 4 cut(s) 155, 220, 326, 578
Tru1I TTAA 3 cut(s) 657, 771, 779
Tru9I TTAA 3 cut(s) 657, 771, 779
TscAI CASTG 2 cut(s) 106, 673
TseI GCWGC 2 cut(s) 701, 977
TspDTI ATGAA 1 cut(s) 450
TspRI CASTG 2 cut(s) 106, 673
XagI CCTNNNNNAGG 1 cut(s) 375
XapI RAATTY 2 cut(s) 904, 951
XbaI TCTAGA 1 cut(s) 464
XceI RCATGY 1 cut(s) 683
XcmI CCANNNNNNNNNTGG 1 cut(s) 870
XspI CTAG 5 cut(s) 204, 465, 602, 869, 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.