MD12G1023800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014416
Physical Location & Seq
Forward (+)
2399630 .. 2400061
432 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1023800.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGCTTCTCTCGTTGGAGGTTCCGCTTCTGCGCAACGTGCCGGACCGGACATGGAAGACGAAGACGATCTCTTCGAGATTGATCTCGATTCGGTTGACAGCATTCCTCAAACACAGTACTACTGGAAGAGCTATTTCACAGAGACCGGAAATGCATTGCTTGCCAATTGCCTACTGCCGGTCACTGACATCTCCCGTGCCATCCCCATGGTTTCGAAGGCACTGTCGTCGTCGGAGCGGACTGGCAATGTTGTAATGATCCCACAGGCCGTGCCCTTGGGGCAGCTTCTAGGGTTGCCATTCCTGGAGGCTTTTGGGCTTCACCAAAAGGAAATGAAACAAATGTGCAATCCGATTAGGAAAACTGAAGCTCATTTGGATGTGGGGCCTGAGCCACTAGAAGGAAGGTATCACTTTGTTTATGGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

144

Amino Acids

15.75

Weight (kDa)

4.72

Isoelectric Point (pI)

31.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014416)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36295
fragaria_vesca FvH4_6g18470
malus_domestica MD12G1023800.v1.1 MD14G1021000.v1.1
prunus_persica Prupe.7G112700_v2.0.a1
pyrus_communis pycom12g02220 pycom14g02040
rosa_chinensis RchiOBHm_Chr3g0473001
rosa_laevigata RLG00000024038
rosa_multiflora Rmu_sc0010986.1_g000005
rosa_roxburghii Rroxscaffold_6G00408410
rosa_rugosa Rorug03G0130600
rosa_samantha Rh3AG182400 Rh3BG208700 Rh3CG205700 Rh3DG167300
rosa_wichuraiana Rw3G016080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 33
AccBSI CCGCTC 1 cut(s) 238
AciI CCGC 2 cut(s) 24, 238
AclWI GGATC 1 cut(s) 253
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 1 cut(s) 119
AfiI CCNNNNNNNGG 2 cut(s) 178, 401
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 3 cut(s) 132, 286, 371
AluI AGCT 3 cut(s) 132, 286, 371
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 1 cut(s) 253
AoxI GGCC 2 cut(s) 267, 386
ApeKI GCWGC 1 cut(s) 283
ArsI GACNNNNNNTTYG 4 cut(s) 56, 88, 209, 241
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 2 cut(s) 44, 386
AsuHPI GGTGA 1 cut(s) 314
AsuII TTCGAA 1 cut(s) 215
AvaII GGWCC 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 276
BbsI GAAGAC 2 cut(s) 63, 69
BbvI GCAGC 1 cut(s) 295
BccI CCATC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 254
BcgI CGANNNNNNTGC 2 cut(s) 210, 244
BciT130I CCWGG 1 cut(s) 305
BcoDI GTCTC 1 cut(s) 137
BfaI CTAG 2 cut(s) 290, 398
BglI GCCNNNNNGGC 1 cut(s) 280
BisI GCNGC 1 cut(s) 284
BlsI GCNGC 1 cut(s) 285
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 305
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 44, 386
BmiI GGNNCC 2 cut(s) 22, 387
BmrFI CCNGG 1 cut(s) 305
BpiI GAAGAC 2 cut(s) 63, 69
BpmI CTGGAG 1 cut(s) 326
Bpu10I CCTNAGC 1 cut(s) 390
Bpu14I TTCGAA 1 cut(s) 215
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 207, 276
BsaWI WCCGGW 2 cut(s) 46, 146
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 401
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 2 cut(s) 128, 247
Bse3DI GCAATG 2 cut(s) 155, 253
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 2 cut(s) 207, 276
BseGI GGATG 2 cut(s) 201, 385
BseLI CCNNNNNNNGG 2 cut(s) 178, 401
BseMI GCAATG 2 cut(s) 155, 253
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 2 cut(s) 128, 247
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 1 cut(s) 295
BshFI GGCC 2 cut(s) 269, 388
BsiSI CCGG 4 cut(s) 42, 47, 147, 179
BslI CCNNNNNNNGG 2 cut(s) 178, 401
BsmAI GTCTC 1 cut(s) 137
BsmI GAATGC 1 cut(s) 102
BsnI GGCC 2 cut(s) 269, 388
Bso31I GGTCTC 1 cut(s) 137
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 3 cut(s) 67, 82, 258
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 2 cut(s) 24, 238
BspANI GGCC 2 cut(s) 269, 388
BspCNI CTCAG 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 22, 387
BspPI GGATC 1 cut(s) 253
BspQI GCTCTTC 1 cut(s) 122
BspT104I TTCGAA 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 137
BsrBI CCGCTC 1 cut(s) 238
BsrDI GCAATG 2 cut(s) 155, 253
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 2 cut(s) 128, 247
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 207, 276
BssMI GATC 3 cut(s) 67, 82, 258
BssT1I CCWWGG 2 cut(s) 207, 276
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 2 cut(s) 117, 225
Bst6I CTCTTC 2 cut(s) 77, 122
BstAPI GCANNNNNTGC 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 2 cut(s) 201, 385
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 3 cut(s) 70, 85, 261
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 3 cut(s) 67, 82, 258
BstMWI GCNNNNNNNGC 3 cut(s) 38, 161, 280
BstNI CCWGG 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 303
BstSLI GKGCMC 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 295
BstV2I GAAGAC 2 cut(s) 63, 69
BstXI CCANNNNNNTGG 1 cut(s) 208
BsuRI GGCC 2 cut(s) 269, 388
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 2 cut(s) 201, 385
BtsIMutI CAGTG 2 cut(s) 183, 221
Cac8I GCNNGC 1 cut(s) 162
CfoI GCGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 2 cut(s) 44, 386
CpoI CGGWCCG 1 cut(s) 44
Csp6I GTAC 1 cut(s) 118
CspI CGGWCCG 1 cut(s) 44
CviAII CATG 2 cut(s) 52, 208
CviJI RGCY 9 cut(s) 5, 132, 269, 286, 311, 319, 371, 388, 394
CviKI_1 RGCY 9 cut(s) 5, 132, 269, 286, 311, 319, 371, 388, 394
CviQI GTAC 1 cut(s) 118
DdeI CTNAG 1 cut(s) 390
DpnI GATC 3 cut(s) 69, 84, 260
DpnII GATC 3 cut(s) 67, 82, 258
Eam1104I CTCTTC 2 cut(s) 77, 122
EarI CTCTTC 2 cut(s) 77, 122
Eco130I CCWWGG 2 cut(s) 207, 276
Eco31I GGTCTC 1 cut(s) 137
Eco47I GGWCC 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 303
EcoT14I CCWWGG 2 cut(s) 207, 276
EcoT22I ATGCAT 1 cut(s) 157
ErhI CCWWGG 2 cut(s) 207, 276
FaeI CATG 2 cut(s) 55, 211
FaiI YATR 3 cut(s) 53, 209, 423
FatI CATG 2 cut(s) 51, 207
Fnu4HI GCNGC 1 cut(s) 284
FokI GGATG 2 cut(s) 188, 392
Fsp4HI GCNGC 1 cut(s) 284
FspBI CTAG 2 cut(s) 290, 398
FspI TGCGCA 1 cut(s) 33
GlaI GCGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 284
GsuI CTGGAG 1 cut(s) 326
HaeIII GGCC 2 cut(s) 269, 388
HapII CCGG 4 cut(s) 42, 47, 147, 179
HhaI GCGC 1 cut(s) 34
Hin1II CATG 2 cut(s) 55, 211
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 1 cut(s) 89
HpaII CCGG 4 cut(s) 42, 47, 147, 179
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 2 cut(s) 235, 354
Hpy188III TCNNGA 2 cut(s) 76, 86
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 2 cut(s) 232, 235
HpyAV CCTTC 3 cut(s) 211, 395, 399
HpyCH4III ACNGT 2 cut(s) 117, 225
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 2 cut(s) 155, 348
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 161, 280
HpyF3I CTNAG 1 cut(s) 390
HpySE526I ACGT 1 cut(s) 37
Hsp92II CATG 2 cut(s) 55, 211
HspAI GCGC 1 cut(s) 32
Kzo9I GATC 3 cut(s) 67, 82, 258
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 1 cut(s) 235
Lsp1109I GCAGC 1 cut(s) 295
MaeI CTAG 2 cut(s) 290, 398
MaeII ACGT 1 cut(s) 37
MaeIII GTNAC 1 cut(s) 181
MalI GATC 3 cut(s) 69, 84, 260
MbiI CCGCTC 1 cut(s) 238
MboI GATC 3 cut(s) 67, 82, 258
MboII GAAGA 4 cut(s) 64, 68, 74, 139
MfeI CAATTG 1 cut(s) 166
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 1 cut(s) 166
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 3 cut(s) 11, 117, 301
Mph1103I ATGCAT 1 cut(s) 157
MslI CAYNNNNRTG 2 cut(s) 206, 378
MspI CCGG 4 cut(s) 42, 47, 147, 179
MspR9I CCNGG 1 cut(s) 305
MunI CAATTG 1 cut(s) 166
Mva1269I GAATGC 1 cut(s) 102
MvaI CCWGG 1 cut(s) 305
MwoI GCNNNNNNNGC 3 cut(s) 38, 161, 280
NcoI CCATGG 1 cut(s) 207
NdeII GATC 3 cut(s) 67, 82, 258
NlaIII CATG 2 cut(s) 55, 211
NlaIV GGNNCC 2 cut(s) 22, 387
NmuCI GTSAC 1 cut(s) 181
NsbI TGCGCA 1 cut(s) 33
NsiI ATGCAT 1 cut(s) 157
NspV TTCGAA 1 cut(s) 215
PciSI GCTCTTC 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 1 cut(s) 102
PfeI GAWTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 303
PkrI GCNGC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 2 cut(s) 22, 387
PspPI GGNCC 2 cut(s) 44, 386
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
RseI CAYNNNNRTG 2 cut(s) 206, 378
Rsr2I CGGWCCG 1 cut(s) 44
RsrII CGGWCCG 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 122
SatI GCNGC 1 cut(s) 284
Sau3AI GATC 3 cut(s) 67, 82, 258
Sau96I GGNCC 2 cut(s) 44, 386
ScaI AGTACT 1 cut(s) 119
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 1 cut(s) 276
SetI ASST 6 cut(s) 22, 40, 134, 288, 373, 410
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 1 cut(s) 44
SmiMI CAYNNNNRTG 2 cut(s) 206, 378
Sse9I AATT 1 cut(s) 166
SsiI CCGC 2 cut(s) 24, 238
SspMI CTAG 2 cut(s) 290, 398
StyD4I CCNGG 1 cut(s) 303
StyI CCWWGG 2 cut(s) 207, 276
TaaI ACNGT 2 cut(s) 117, 225
TaiI ACGT 1 cut(s) 40
TaqI TCGA 3 cut(s) 75, 87, 215
TasI AATT 1 cut(s) 166
TatI WGTACW 1 cut(s) 117
TfiI GAWTC 1 cut(s) 89
TscAI CASTG 2 cut(s) 190, 228
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 1 cut(s) 350
TspRI CASTG 2 cut(s) 190, 228
VpaK11BI GGWCC 1 cut(s) 44
XspI CTAG 2 cut(s) 290, 398
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.