pycom12g02220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014416
Physical Location & Seq
Forward (+)
1931491 .. 1931799
309 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02220.1

Sequence Viewer

Length: 309 bp
ATGGCTTCTCCCGTTGGAGGTTCTGCTTCCGCGCAAGGTGCCGGACTGTGTAAGGGCCTACCGGACATGGAAGACGAAGATGATCTCTTCGAGATTGATCTCGATTCAGTTGACAGCATTCCTCAAACACAGTACTACTGGGACAGCTATTTCACAGAGACCGGAAATGCATTGCTTGCCAATTGCCTACTGCCCGTCACCGACATCTCCCGTGCCATCCCGATGGTTTCGAAGGCACTATCGTCGTCGGAGCAGACTGGCGATGTTGCAATGATCCCACTTAGGCTGTGCCCTTGGGGCAGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

10.89

Weight (kDa)

4.05

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014416)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36295
fragaria_vesca FvH4_6g18470
malus_domestica MD12G1023800.v1.1 MD14G1021000.v1.1
prunus_persica Prupe.7G112700_v2.0.a1
pyrus_communis pycom12g02220 pycom14g02040
rosa_chinensis RchiOBHm_Chr3g0473001
rosa_laevigata RLG00000024038
rosa_multiflora Rmu_sc0010986.1_g000005
rosa_roxburghii Rroxscaffold_6G00408410
rosa_rugosa Rorug03G0130600
rosa_samantha Rh3AG182400 Rh3BG208700 Rh3CG205700 Rh3DG167300
rosa_wichuraiana Rw3G016080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 38
AccII CGCG 1 cut(s) 32
AciI CCGC 1 cut(s) 30
AclWI GGATC 1 cut(s) 268
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 17
AluBI AGCT 2 cut(s) 147, 303
AluI AGCT 2 cut(s) 147, 303
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 1 cut(s) 268
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 300
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 55
AsuHPI GGTGA 1 cut(s) 190
AsuII TTCGAA 1 cut(s) 230
BaeGI GKGCMC 1 cut(s) 293
BanI GGYRCC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 78
BccI CCATC 2 cut(s) 217, 224
BcgI CGANNNNNNTGC 2 cut(s) 225, 259
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 307
BglI GCCNNNNNGGC 1 cut(s) 297
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
BmcAI AGTACT 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 40
BmrI ACTGGG 1 cut(s) 148
BmuI ACTGGG 1 cut(s) 148
BpiI GAAGAC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 230
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 293
BsaWI WCCGGW 2 cut(s) 61, 161
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 2 cut(s) 143, 262
Bse3DI GCAATG 2 cut(s) 170, 276
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 1 cut(s) 216
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMI GCAATG 2 cut(s) 170, 276
BseNI ACTGG 2 cut(s) 143, 262
BseSI GKGCMC 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 32
BshFI GGCC 1 cut(s) 57
BshNI GGYRCC 1 cut(s) 38
BsiSI CCGG 3 cut(s) 42, 62, 162
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 155
BsmI GAATGC 1 cut(s) 117
BsnI GGCC 1 cut(s) 57
Bso31I GGTCTC 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 293
Bsp143I GATC 3 cut(s) 82, 97, 273
BspACI CCGC 1 cut(s) 30
BspANI GGCC 1 cut(s) 57
BspFNI CGCG 1 cut(s) 32
BspLI GGNNCC 1 cut(s) 40
BspPI GGATC 1 cut(s) 268
BspT104I TTCGAA 1 cut(s) 230
BspT107I GGYRCC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 152
BsrDI GCAATG 2 cut(s) 170, 276
BsrI ACTGG 2 cut(s) 143, 262
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 3 cut(s) 82, 97, 273
BssT1I CCWWGG 1 cut(s) 293
Bst4CI ACNGT 2 cut(s) 48, 132
Bst6I CTCTTC 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 176
BstBI TTCGAA 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 1 cut(s) 281
BstF5I GGATG 1 cut(s) 216
BstFNI CGCG 1 cut(s) 32
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 3 cut(s) 85, 100, 276
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 3 cut(s) 82, 97, 273
BstMWI GCNNNNNNNGC 3 cut(s) 38, 176, 297
BstSLI GKGCMC 1 cut(s) 293
BstUI CGCG 1 cut(s) 32
BstV2I GAAGAC 1 cut(s) 78
BstXI CCANNNNNNTGG 1 cut(s) 223
BsuRI GGCC 1 cut(s) 57
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 1 cut(s) 216
Cac8I GCNNGC 1 cut(s) 177
CfoI GCGC 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 55
Csp6I GTAC 1 cut(s) 133
CviAII CATG 1 cut(s) 67
CviJI RGCY 5 cut(s) 5, 57, 147, 286, 303
CviKI_1 RGCY 5 cut(s) 5, 57, 147, 286, 303
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 1 cut(s) 281
DpnI GATC 3 cut(s) 84, 99, 275
DpnII GATC 3 cut(s) 82, 97, 273
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 293
Eco31I GGTCTC 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 293
EcoT22I ATGCAT 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 293
FaeI CATG 1 cut(s) 70
FaiI YATR 1 cut(s) 68
FaqI GGGAC 1 cut(s) 155
FatI CATG 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 1 cut(s) 301
FspBI CTAG 1 cut(s) 307
GlaI GCGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 1 cut(s) 57
HapII CCGG 3 cut(s) 42, 62, 162
HhaI GCGC 1 cut(s) 34
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HinfI GANTC 1 cut(s) 104
HpaII CCGG 3 cut(s) 42, 62, 162
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 1 cut(s) 250
Hpy188III TCNNGA 3 cut(s) 91, 101, 220
Hpy8I GTNNAC 1 cut(s) 112
Hpy99I CGWCG 2 cut(s) 247, 250
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 2 cut(s) 48, 132
HpyCH4V TGCA 2 cut(s) 170, 269
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 176, 297
HpyF3I CTNAG 1 cut(s) 281
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 1 cut(s) 32
Kzo9I GATC 3 cut(s) 82, 97, 273
LmnI GCTCC 1 cut(s) 250
LpnPI CCDG 5 cut(s) 55, 75, 124, 175, 243
MaeI CTAG 1 cut(s) 307
MaeIII GTNAC 1 cut(s) 196
MalI GATC 3 cut(s) 84, 99, 275
MboI GATC 3 cut(s) 82, 97, 273
MboII GAAGA 3 cut(s) 79, 83, 89
MfeI CAATTG 1 cut(s) 181
MhlI GDGCHC 1 cut(s) 293
MluCI AATT 1 cut(s) 181
MmeI TCCRAC 1 cut(s) 228
MnlI CCTC 2 cut(s) 11, 132
Mph1103I ATGCAT 1 cut(s) 172
MslI CAYNNNNRTG 1 cut(s) 221
MspI CCGG 3 cut(s) 42, 62, 162
MunI CAATTG 1 cut(s) 181
Mva1269I GAATGC 1 cut(s) 117
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 3 cut(s) 38, 176, 297
NdeII GATC 3 cut(s) 82, 97, 273
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 1 cut(s) 40
NmuCI GTSAC 1 cut(s) 196
NsiI ATGCAT 1 cut(s) 172
NspV TTCGAA 1 cut(s) 230
PctI GAATGC 1 cut(s) 117
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 40
PspPI GGNCC 1 cut(s) 55
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 221
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 3 cut(s) 82, 97, 273
Sau96I GGNCC 1 cut(s) 55
ScaI AGTACT 1 cut(s) 134
SduI GDGCHC 1 cut(s) 293
SetI ASST 4 cut(s) 22, 40, 149, 305
SfuI TTCGAA 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 221
Sse9I AATT 1 cut(s) 181
SsiI CCGC 1 cut(s) 30
SspMI CTAG 1 cut(s) 307
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 2 cut(s) 48, 132
TaqI TCGA 3 cut(s) 90, 102, 230
TasI AATT 1 cut(s) 181
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 1 cut(s) 104
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 196
XspI CTAG 1 cut(s) 307
ZrmI AGTACT 1 cut(s) 134
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.