MD12G1037400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011416
Physical Location & Seq
Forward (+)
4006567 .. 4008917
2351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1037400.v1.1.491

Sequence Viewer

Length: 441 bp
ATGGAAGGAGTTCTGCATTCTTCAACACTAACCCTTCCCATTACCTGTCATCATATCTCCAAAACCCAGATCCTGAAAACAGCCAACCACAACCCCACGCAAAGGCCAATGCTAATGATCTCCACGTTCCCAGCAACACTGGGAACCAAAACTAGCACGAAATTCTCAACAATATTGGCAGCAGCAGCGGTTCAGCCTTCGCAGCCTCTCGATCTAACGGAAGACAATATTCGACAGGTCTTAGCCGATGCAAGGGTTGAATTCGGGCAGCTCTTCGACACTTCAGTTGGCATGACAGGACAAGTAGACCTTGCTGAACTTGACGGACCCTTTGTGAAGATCAGTCTGAAAGGCCGGTTTTGGCACGAACGGAGCGTGGTTCTGGCTAGACTGGCCAATTATCTCAAGCAGAGGATCCCTGTGAGAAATTTTAGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

16.17

Weight (kDa)

9.8

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011416)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33180
fragaria_vesca FvH4_6g20030 FvH4_6g20030 FvH4_6g20030
malus_domestica MD12G1037400.v1.1
prunus_persica Prupe.7G097800_v2.0.a1 Prupe.7G097800_v2.0.a1
pyrus_communis pycom12g03240
rosa_chinensis RchiOBHm_Chr3g0475481
rosa_laevigata RLG00000023841
rosa_multiflora Rmu_sc0015717.1_g000002 Rmu_sc0016637.1_g000011
rosa_roxburghii Rroxscaffold_6G00406280
rosa_rugosa Rorug03G0149100 Rorug03G0149200
rosa_samantha Rh3AG199400 Rh3BG229200 Rh3CG223600 Rh3DG224500
rosa_wichuraiana Rw3G018080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 306
AciI CCGC 1 cut(s) 188
AclWI GGATC 3 cut(s) 64, 409, 422
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 3 cut(s) 161, 260, 427
AcuI CTGAAG 1 cut(s) 267
AfiI CCNNNNNNNGG 2 cut(s) 102, 252
AgsI TTSAA 2 cut(s) 24, 260
AluBI AGCT 1 cut(s) 271
AluI AGCT 1 cut(s) 271
AlwI GGATC 3 cut(s) 64, 409, 422
AlwNI CAGNNNCTG 1 cut(s) 73
AoxI GGCC 3 cut(s) 104, 352, 393
ApeKI GCWGC 5 cut(s) 179, 182, 185, 202, 268
ApoI RAATTY 3 cut(s) 161, 260, 427
ArsI GACNNNNNNTTYG 2 cut(s) 314, 346
Asp700I GAANNNNTTC 1 cut(s) 9
AspS9I GGNCC 1 cut(s) 326
AvaII GGWCC 1 cut(s) 326
BalI TGGCCA 1 cut(s) 395
BamHI GGATCC 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 228
BbvI GCAGC 5 cut(s) 191, 194, 197, 214, 280
BfaI CTAG 2 cut(s) 153, 387
BisI GCNGC 5 cut(s) 180, 183, 186, 203, 269
BlsI GCNGC 5 cut(s) 181, 184, 187, 204, 270
Bme18I GGWCC 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 3 cut(s) 145, 328, 416
BmrI ACTGGG 1 cut(s) 149
BmsI GCATC 1 cut(s) 238
BmuI ACTGGG 1 cut(s) 149
BpiI GAAGAC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 389
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 252
Bse118I RCCGGY 1 cut(s) 354
Bse1I ACTGG 2 cut(s) 144, 396
BseLI CCNNNNNNNGG 2 cut(s) 102, 252
BseNI ACTGG 2 cut(s) 144, 396
BseXI GCAGC 5 cut(s) 191, 194, 197, 214, 280
BseYI CCCAGC 1 cut(s) 130
BshFI GGCC 3 cut(s) 106, 354, 395
BsiSI CCGG 1 cut(s) 355
BslI CCNNNNNNNGG 2 cut(s) 102, 252
BsmI GAATGC 1 cut(s) 16
BsnI GGCC 3 cut(s) 106, 354, 395
Bsp143I GATC 5 cut(s) 69, 117, 211, 339, 414
BspACI CCGC 1 cut(s) 188
BspANI GGCC 3 cut(s) 106, 354, 395
BspLI GGNNCC 3 cut(s) 145, 328, 416
BspPI GGATC 3 cut(s) 64, 409, 422
BspQI GCTCTTC 1 cut(s) 278
BsrFI RCCGGY 1 cut(s) 354
BsrI ACTGG 2 cut(s) 144, 396
BssAI RCCGGY 1 cut(s) 354
BssMI GATC 5 cut(s) 69, 117, 211, 339, 414
Bst6I CTCTTC 1 cut(s) 278
BstDEI CTNAG 1 cut(s) 241
BstKTI GATC 5 cut(s) 72, 120, 214, 342, 417
BstMBI GATC 5 cut(s) 69, 117, 211, 339, 414
BstMWI GCNNNNNNNGC 3 cut(s) 185, 202, 392
BstV1I GCAGC 5 cut(s) 191, 194, 197, 214, 280
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 2 cut(s) 69, 414
BstYI RGATCY 2 cut(s) 69, 414
BsuRI GGCC 3 cut(s) 106, 354, 395
BtsIMutI CAGTG 1 cut(s) 137
CaiI CAGNNNCTG 1 cut(s) 73
Cfr10I RCCGGY 1 cut(s) 354
Cfr13I GGNCC 1 cut(s) 326
CviAII CATG 1 cut(s) 292
CviJI RGCY 9 cut(s) 83, 106, 196, 205, 245, 271, 354, 386, 395
CviKI_1 RGCY 9 cut(s) 83, 106, 196, 205, 245, 271, 354, 386, 395
DdeI CTNAG 1 cut(s) 241
DpnI GATC 5 cut(s) 71, 119, 213, 341, 416
DpnII GATC 5 cut(s) 69, 117, 211, 339, 414
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
Eco47I GGWCC 1 cut(s) 326
Eco57I CTGAAG 1 cut(s) 267
EcoRI GAATTC 1 cut(s) 260
FaeI CATG 1 cut(s) 295
FaiI YATR 2 cut(s) 54, 293
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FatI CATG 1 cut(s) 291
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 5 cut(s) 180, 183, 186, 203, 269
Fsp4HI GCNGC 5 cut(s) 180, 183, 186, 203, 269
FspBI CTAG 2 cut(s) 153, 387
GluI GCNGC 5 cut(s) 180, 183, 186, 203, 269
GsaI CCCAGC 1 cut(s) 134
HaeIII GGCC 3 cut(s) 106, 354, 395
HapII CCGG 1 cut(s) 355
Hin1II CATG 1 cut(s) 295
HpaII CCGG 1 cut(s) 355
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 1 cut(s) 348
Hpy188III TCNNGA 2 cut(s) 73, 209
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 2 cut(s) 44, 207
HpyCH4IV ACGT 1 cut(s) 125
HpyCH4V TGCA 2 cut(s) 16, 251
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 202, 392
HpyF3I CTNAG 1 cut(s) 241
HpySE526I ACGT 1 cut(s) 125
Hsp92II CATG 1 cut(s) 295
Kzo9I GATC 5 cut(s) 69, 117, 211, 339, 414
LguI GCTCTTC 1 cut(s) 278
LmnI GCTCC 1 cut(s) 372
Lsp1109I GCAGC 5 cut(s) 191, 194, 197, 214, 280
LweI GCATC 1 cut(s) 238
MaeI CTAG 2 cut(s) 153, 387
MaeII ACGT 1 cut(s) 125
MalI GATC 5 cut(s) 71, 119, 213, 341, 416
MboI GATC 5 cut(s) 69, 117, 211, 339, 414
MboII GAAGA 4 cut(s) 12, 233, 265, 349
MflI RGATCY 2 cut(s) 69, 414
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 4 cut(s) 161, 260, 397, 427
MluNI TGGCCA 1 cut(s) 395
MnlI CCTC 3 cut(s) 216, 405, 428
Mox20I TGGCCA 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 9
MscI TGGCCA 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 355
Mva1269I GAATGC 1 cut(s) 16
MwoI GCNNNNNNNGC 3 cut(s) 185, 202, 392
NdeII GATC 5 cut(s) 69, 117, 211, 339, 414
NlaIII CATG 1 cut(s) 295
NlaIV GGNNCC 3 cut(s) 145, 328, 416
PciSI GCTCTTC 1 cut(s) 278
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PctI GAATGC 1 cut(s) 16
PdmI GAANNNNTTC 1 cut(s) 9
PkrI GCNGC 5 cut(s) 181, 184, 187, 204, 270
PspFI CCCAGC 1 cut(s) 130
PspN4I GGNNCC 3 cut(s) 145, 328, 416
PspPI GGNCC 1 cut(s) 326
PstNI CAGNNNCTG 1 cut(s) 73
PsuI RGATCY 2 cut(s) 69, 414
SapI GCTCTTC 1 cut(s) 278
SatI GCNGC 5 cut(s) 180, 183, 186, 203, 269
Sau3AI GATC 5 cut(s) 69, 117, 211, 339, 414
Sau96I GGNCC 1 cut(s) 326
SetI ASST 6 cut(s) 47, 128, 240, 273, 312, 439
SfaNI GCATC 1 cut(s) 238
SinI GGWCC 1 cut(s) 326
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 4 cut(s) 161, 260, 397, 427
SsiI CCGC 1 cut(s) 188
SspI AATATT 2 cut(s) 174, 229
SspMI CTAG 2 cut(s) 153, 387
TaiI ACGT 1 cut(s) 128
TaqI TCGA 3 cut(s) 210, 232, 276
TasI AATT 4 cut(s) 161, 260, 397, 427
TscAI CASTG 1 cut(s) 144
TseI GCWGC 5 cut(s) 179, 182, 185, 202, 268
TspGWI ACGGA 3 cut(s) 233, 339, 385
TspRI CASTG 1 cut(s) 144
VpaK11BI GGWCC 1 cut(s) 326
XapI RAATTY 3 cut(s) 161, 260, 427
XmiI GTMKAC 1 cut(s) 306
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 2 cut(s) 153, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.