Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0013868

Physical Location & Seq

Genomic Coordinates Forward (+)

4528126 .. 4529346

Gene Structure 1221 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1040900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1221 bp

ATGGACGAAGAAATTGATGTTCCCTCATTTTTTCTGTGCCCCATTTCTCTAGAAATCATGAAGGATCCCGTCACAATCTCGACCGGGATCACTTACGATCGAGAGAGCATAGAGAAATGGTTGTTCTCAAGCAAGAACAAGACTTGTCCCGTTACCAAACAAGTGGTTTCGGAGGATTCCGACTTAACTCCCAACCACACTCTTCGCCGGTTGCTCCAAGCTTGGTGCACAATGAACGCTTCTCACGGCATTGAAAGGATCCCAACTCCGAAGCCGCCCGTTAACAAATCTCAGATCGATAAGCTCCTCCGAGACGCCAAAAAGTCCCCAAACGGTCTGGCAAAATGCCTCAGTGAGCTTCGATCCATTTCATCCCAAAGCGAAGCCAACAAGAGAAGCATCGAAGCCGCTGGTGCAGGCGAGTTCCTTGCTTCAACGATAATCAACAACATCAAGAACGACAGAGAAAATGTCGAAAACGAAGAAGCTTTGAGCATTCTCTATAACCTAAATCTTAGAGAAGATGTTCTCAAAACTCTAATAGGCAAAGAGGGTGAGTTTGTCGAGGCCTTAATCAAACTCATGCAACGTGGGAGCGACGAGTCTCGGACGTACGCTGTGATGTTGTTGAGATCAATGTTCGAGGTTGCTGATTCGATGAGGATGACAAACGTAAAACCTGCATTCTTCGTCCAAGTTGTTCAGGTCTTAAATGATCAAATCTCACAACAGGCCTCAAAAGCCACATTGCAACTGTTGATCCAGTTCTGTCAATGGGGACGAAACAGAACCAAAGCCGTTGAATCCGGGGCGGTTATGGTCTTGATTGAGCTTCTTCTGGATACTTCTTCCGATAGGAGAACCTTGGAAATGACACTCTTGGTGCTTGACATGCTATGCAGCTGTGCGGAAGGCCGCGCAGAACTGCTTACCCACAGCGCAGGACTAGCCGTGGTGTCTAAGAAGATAATGAGGGTCTCGAAGGTGGCGAGCGAGAGGGCAGTGAAGATTTTGTTCTCCATTTGCAAGTTTTCCCCGACGTCGAATGTTTTGCAGGAGATGATGCAATTAGGTATTGTGGCGAAGCTATGCTTAGTTCTTCAAGTGGATAGTGGGAGCAAGACCAAGGAGAAGGCTAGAGAGATCTTAAAATTGCATGCAAGGACATGGAAGAATTCTACTTGTATACCAAGCAATTTGGTTTCTTCTTATCCATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010033 GO:0010035 GO:0010200 GO:0010243 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051865 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

U-box

Protein Analysis

407

Amino Acids

45.28

Weight (kDa)

8.58

Isoelectric Point (pI)

46.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
U-box	PF04564	7 - 79	1.4e-19	U-box domain
ARM_PUB	PF25598	98 - 404	2.7e-84	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013868)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35930
fragaria_vesca	FvH4_6g20400
malus_domestica	MD12G1040900.v1.1 MD14G1040300.v1.1
prunus_persica	Prupe.7G094000_v2.0.a1
pyrus_communis	pycom12g03520 pycom14g03420
rosa_chinensis	RchiOBHm_Chr3g0476101
rosa_laevigata	RLG00000023790
rosa_multiflora	Rmu_sc0000114.1_g000029
rosa_roxburghii	Rroxscaffold_6G00405470
rosa_rugosa	Rorug03G0152800
rosa_samantha	Rh3AG203800 Rh3BG234100 Rh3CG229400 Rh3DG229300
rosa_wichuraiana	Rw3G018520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1043
Acc36I	ACCTGC	1 cut(s)	688
AccI	GTMKAC	1 cut(s)	1186
AccII	CGCG	1 cut(s)	918
AciI	CCGC	5 cut(s)	275, 408, 812, 908, 916
AclWI	GGATC	7 cut(s)	59, 72, 95, 253, 266, 357, 754
AcsI	RAATTY	1 cut(s)	1174
AcyI	GRCGYC	2 cut(s)	315, 1040
AfaI	GTAC	1 cut(s)	614
AgsI	TTSAA	4 cut(s)	254, 435, 803, 1103
AluBI	AGCT	7 cut(s)	221, 304, 358, 488, 832, 903, 1087
AluI	AGCT	7 cut(s)	221, 304, 358, 488, 832, 903, 1087
Alw21I	GWGCWC	1 cut(s)	230
Alw26I	GTCTC	3 cut(s)	306, 609, 982
Alw44I	GTGCAC	1 cut(s)	226
AlwI	GGATC	7 cut(s)	59, 72, 95, 253, 266, 357, 754
AoxI	GGCC	3 cut(s)	567, 732, 913
ApaLI	GTGCAC	1 cut(s)	226
ApeKI	GCWGC	1 cut(s)	900
ApoI	RAATTY	1 cut(s)	1174
Asp700I	GAANNNNTTC	1 cut(s)	525
AspLEI	GCGC	2 cut(s)	920, 941
AsuC2I	CCSGG	2 cut(s)	85, 808
AsuHPI	GGTGA	1 cut(s)	566
BaeGI	GKGCMC	2 cut(s)	41, 230
BamHI	GGATCC	2 cut(s)	64, 258
Bbv12I	GWGCWC	1 cut(s)	230
BbvI	GCAGC	1 cut(s)	912
BceAI	ACGGC	3 cut(s)	262, 782, 935
BcgI	CGANNNNNNTGC	4 cut(s)	410, 444, 1033, 1067
BciVI	GTATCC	1 cut(s)	835
BclI	TGATCA	1 cut(s)	715
BcnI	CCSGG	2 cut(s)	85, 808
BcoDI	GTCTC	3 cut(s)	306, 609, 982
BfaI	CTAG	3 cut(s)	50, 947, 1137
BfuAI	ACCTGC	1 cut(s)	688
BfuI	GTATCC	1 cut(s)	835
BglII	AGATCT	1 cut(s)	1143
BisI	GCNGC	4 cut(s)	275, 408, 901, 916
BlsI	GCNGC	4 cut(s)	276, 409, 902, 917
Bme1390I	CCNGG	2 cut(s)	85, 808
BmiI	GGNNCC	2 cut(s)	66, 260
BmrFI	CCNGG	2 cut(s)	85, 808
BmsI	GCATC	2 cut(s)	408, 1053
BpuEI	CTTGAG	1 cut(s)	112
BpuMI	CCSGG	2 cut(s)	85, 808
Bsa29I	ATCGAT	1 cut(s)	297
BsaHI	GRCGYC	2 cut(s)	315, 1040
BsaI	GGTCTC	1 cut(s)	982
BsaJI	CCNNGG	4 cut(s)	807, 864, 951, 1125
Bse118I	RCCGGY	1 cut(s)	207
Bse1I	ACTGG	1 cut(s)	763
Bse3DI	GCAATG	1 cut(s)	746
BseCI	ATCGAT	1 cut(s)	297
BseDI	CCNNGG	4 cut(s)	807, 864, 951, 1125
BseGI	GGATG	2 cut(s)	371, 669
BseMI	GCAATG	1 cut(s)	746
BseMII	CTCAG	2 cut(s)	305, 364
BseNI	ACTGG	1 cut(s)	763
BseRI	GAGGAG	1 cut(s)	296
BseSI	GKGCMC	2 cut(s)	41, 230
BseXI	GCAGC	1 cut(s)	912
BsgI	GTGCAG	1 cut(s)	435
Bsh1236I	CGCG	1 cut(s)	918
Bsh1285I	CGRYCG	2 cut(s)	84, 100
BshFI	GGCC	3 cut(s)	569, 734, 915
BshVI	ATCGAT	1 cut(s)	297
BsiEI	CGRYCG	2 cut(s)	84, 100
BsiHKAI	GWGCWC	1 cut(s)	230
BsiSI	CCGG	3 cut(s)	84, 208, 807
BsiWI	CGTACG	1 cut(s)	612
BslFI	GGGAC	3 cut(s)	132, 310, 792
BsmAI	GTCTC	3 cut(s)	306, 609, 982
BsmBI	CGTCTC	1 cut(s)	306
BsmFI	GGGAC	3 cut(s)	132, 310, 792
BsmI	GAATGC	2 cut(s)	495, 683
BsnI	GGCC	3 cut(s)	569, 734, 915
Bso31I	GGTCTC	1 cut(s)	982
Bsp1286I	GDGCHC	2 cut(s)	41, 230
BspACI	CCGC	5 cut(s)	275, 408, 812, 908, 916
BspANI	GGCC	3 cut(s)	569, 734, 915
BspCNI	CTCAG	2 cut(s)	304, 363
BspDI	ATCGAT	1 cut(s)	297
BspFNI	CGCG	1 cut(s)	918
BspHI	TCATGA	1 cut(s)	57
BspLI	GGNNCC	2 cut(s)	66, 260
BspMI	ACCTGC	1 cut(s)	688
BspPI	GGATC	7 cut(s)	59, 72, 95, 253, 266, 357, 754
BspTNI	GGTCTC	1 cut(s)	982
BsrDI	GCAATG	1 cut(s)	746
BsrFI	RCCGGY	1 cut(s)	207
BsrI	ACTGG	1 cut(s)	763
BssAI	RCCGGY	1 cut(s)	207
BssECI	CCNNGG	4 cut(s)	807, 864, 951, 1125
BssNAI	GTATAC	1 cut(s)	1187
BssNI	GRCGYC	2 cut(s)	315, 1040
BssT1I	CCWWGG	2 cut(s)	864, 1125
Bst1107I	GTATAC	1 cut(s)	1187
Bst4CI	ACNGT	2 cut(s)	335, 756
Bst6I	CTCTTC	1 cut(s)	207
BstACI	GRCGYC	2 cut(s)	315, 1040
BstC8I	GCNNGC	3 cut(s)	418, 991, 1158
BstDEI	CTNAG	5 cut(s)	291, 350, 515, 960, 1093
BstDSI	CCRYGG	1 cut(s)	951
BstF5I	GGATG	2 cut(s)	371, 669
BstFNI	CGCG	1 cut(s)	918
BstHHI	GCGC	2 cut(s)	920, 941
BstMAI	GTCTC	3 cut(s)	306, 609, 982
BstMCI	CGRYCG	2 cut(s)	84, 100
BstMWI	GCNNNNNNNGC	4 cut(s)	413, 740, 892, 947
BstNSI	RCATGY	2 cut(s)	895, 1160
BstSCI	CCNGG	2 cut(s)	83, 806
BstSLI	GKGCMC	2 cut(s)	41, 230
BstUI	CGCG	1 cut(s)	918
BstV1I	GCAGC	1 cut(s)	912
BstX2I	RGATCY	3 cut(s)	64, 258, 1143
BstXI	CCANNNNNNTGG	1 cut(s)	163
BstYI	RGATCY	3 cut(s)	64, 258, 1143
BstZ17I	GTATAC	1 cut(s)	1187
Bsu15I	ATCGAT	1 cut(s)	297
BsuI	GTATCC	1 cut(s)	835
BsuRI	GGCC	3 cut(s)	569, 734, 915
BsuTUI	ATCGAT	1 cut(s)	297
BtgI	CCRYGG	1 cut(s)	951
BtsCI	GGATG	2 cut(s)	371, 669
BtsI	GCAGTG	1 cut(s)	1008
BtsIMutI	CAGTG	2 cut(s)	358, 1008
BveI	ACCTGC	1 cut(s)	688
Cac8I	GCNNGC	3 cut(s)	418, 991, 1158
CciI	TCATGA	1 cut(s)	57
CfoI	GCGC	2 cut(s)	920, 941
Cfr10I	RCCGGY	1 cut(s)	207
ClaI	ATCGAT	1 cut(s)	297
CseI	GACGC	1 cut(s)	323
Csp6I	GTAC	1 cut(s)	613
CviAII	CATG	5 cut(s)	58, 583, 892, 1157, 1167
CviQI	GTAC	1 cut(s)	613
DdeI	CTNAG	5 cut(s)	291, 350, 515, 960, 1093
Eam1104I	CTCTTC	1 cut(s)	207
EarI	CTCTTC	1 cut(s)	207
Eco130I	CCWWGG	2 cut(s)	864, 1125
Eco147I	AGGCCT	2 cut(s)	569, 734
Eco31I	GGTCTC	1 cut(s)	982
EcoRI	GAATTC	1 cut(s)	1174
EcoT14I	CCWWGG	2 cut(s)	864, 1125
ErhI	CCWWGG	2 cut(s)	864, 1125
Esp3I	CGTCTC	1 cut(s)	306
FaeI	CATG	5 cut(s)	61, 586, 895, 1160, 1170
FalI	AAGNNNNNCTT	2 cut(s)	1076, 1108
FaqI	GGGAC	3 cut(s)	132, 310, 792
FatI	CATG	5 cut(s)	57, 582, 891, 1156, 1166
FbaI	TGATCA	1 cut(s)	715
FblI	GTMKAC	1 cut(s)	1186
Fnu4HI	GCNGC	4 cut(s)	275, 408, 901, 916
FokI	GGATG	2 cut(s)	358, 676
Fsp4HI	GCNGC	4 cut(s)	275, 408, 901, 916
FspBI	CTAG	3 cut(s)	50, 947, 1137
GlaI	GCGC	2 cut(s)	919, 940
GluI	GCNGC	4 cut(s)	275, 408, 901, 916
HaeIII	GGCC	3 cut(s)	569, 734, 915
HapII	CCGG	3 cut(s)	84, 208, 807
HgaI	GACGC	1 cut(s)	323
HhaI	GCGC	2 cut(s)	920, 941
Hin1I	GRCGYC	2 cut(s)	315, 1040
Hin1II	CATG	5 cut(s)	61, 586, 895, 1160, 1170
Hin6I	GCGC	2 cut(s)	918, 939
HinP1I	GCGC	2 cut(s)	918, 939
HincII	GTYRAC	1 cut(s)	283
HindII	GTYRAC	1 cut(s)	283
HindIII	AAGCTT	2 cut(s)	219, 486
HinfI	GANTC	4 cut(s)	176, 602, 653, 803
HpaI	GTTAAC	1 cut(s)	283
HpaII	CCGG	3 cut(s)	84, 208, 807
HphI	GGTGA	1 cut(s)	566
Hpy166II	GTNNAC	3 cut(s)	228, 283, 1187
Hpy188I	TCNGA	7 cut(s)	172, 181, 270, 294, 311, 609, 853
Hpy188III	TCNNGA	9 cut(s)	50, 58, 79, 101, 454, 823, 839, 979, 1218
Hpy8I	GTNNAC	3 cut(s)	228, 283, 1187
Hpy99I	CGWCG	3 cut(s)	602, 1042, 1045
HpyAV	CCTTC	4 cut(s)	55, 905, 976, 1126
HpyCH4III	ACNGT	2 cut(s)	335, 756
HpyCH4IV	ACGT	4 cut(s)	589, 611, 672, 1040
HpyF10VI	GCNNNNNNNGC	4 cut(s)	413, 740, 892, 947
HpyF3I	CTNAG	5 cut(s)	291, 350, 515, 960, 1093
HpySE526I	ACGT	4 cut(s)	589, 611, 672, 1040
Hsp92I	GRCGYC	2 cut(s)	315, 1040
Hsp92II	CATG	5 cut(s)	61, 586, 895, 1160, 1170
HspAI	GCGC	2 cut(s)	918, 939
Ksp22I	TGATCA	1 cut(s)	715
KspAI	GTTAAC	1 cut(s)	283
LmnI	GCTCC	4 cut(s)	219, 309, 594, 1116
Lsp1109I	GCAGC	1 cut(s)	912
LweI	GCATC	2 cut(s)	408, 1053
MaeI	CTAG	3 cut(s)	50, 947, 1137
MaeII	ACGT	4 cut(s)	589, 611, 672, 1040
MaeIII	GTNAC	2 cut(s)	70, 151
MflI	RGATCY	3 cut(s)	64, 258, 1143
MhlI	GDGCHC	2 cut(s)	41, 230
MluCI	AATT	5 cut(s)	12, 1067, 1151, 1174, 1195
MlyI	GAGTC	1 cut(s)	611
MmeI	TCCRAC	1 cut(s)	204
MroXI	GAANNNNTTC	1 cut(s)	525
MseI	TTAA	5 cut(s)	185, 282, 572, 710, 1148
MspA1I	CMGCKG	2 cut(s)	410, 903
MspI	CCGG	3 cut(s)	84, 208, 807
MspR9I	CCNGG	2 cut(s)	85, 808
Mva1269I	GAATGC	2 cut(s)	495, 683
MvnI	CGCG	1 cut(s)	918
MwoI	GCNNNNNNNGC	4 cut(s)	413, 740, 892, 947
NciI	CCSGG	2 cut(s)	85, 808
NlaIII	CATG	5 cut(s)	61, 586, 895, 1160, 1170
NlaIV	GGNNCC	2 cut(s)	66, 260
NmuCI	GTSAC	1 cut(s)	70
NspI	RCATGY	2 cut(s)	895, 1160
PaeI	GCATGC	1 cut(s)	1160
PagI	TCATGA	1 cut(s)	57
PceI	AGGCCT	2 cut(s)	569, 734
PcsI	WCGNNNNNNNCGW	1 cut(s)	986
PctI	GAATGC	2 cut(s)	495, 683
PdmI	GAANNNNTTC	1 cut(s)	525
PfeI	GAWTC	3 cut(s)	176, 653, 803
Pfl23II	CGTACG	1 cut(s)	612
PkrI	GCNGC	4 cut(s)	276, 409, 902, 917
Ple19I	CGATCG	1 cut(s)	100
PleI	GAGTC	1 cut(s)	610
PpsI	GAGTC	1 cut(s)	610
PspLI	CGTACG	1 cut(s)	612
PspN4I	GGNNCC	2 cut(s)	66, 260
PsuI	RGATCY	3 cut(s)	64, 258, 1143
PvuI	CGATCG	1 cut(s)	100
PvuII	CAGCTG	1 cut(s)	903
RsaI	GTAC	1 cut(s)	614
RsaNI	GTAC	1 cut(s)	613
SaqAI	TTAA	5 cut(s)	185, 282, 572, 710, 1148
SatI	GCNGC	4 cut(s)	275, 408, 901, 916
SchI	GAGTC	1 cut(s)	611
ScrFI	CCNGG	2 cut(s)	85, 808
SduI	GDGCHC	2 cut(s)	41, 230
SfaNI	GCATC	2 cut(s)	408, 1053
SmlI	CTYRAG	1 cut(s)	127
SmoI	CTYRAG	1 cut(s)	127
SphI	GCATGC	1 cut(s)	1160
Sse9I	AATT	5 cut(s)	12, 1067, 1151, 1174, 1195
SseBI	AGGCCT	2 cut(s)	569, 734
SsiI	CCGC	5 cut(s)	275, 408, 812, 908, 916
SspMI	CTAG	3 cut(s)	50, 947, 1137
StuI	AGGCCT	2 cut(s)	569, 734
StyD4I	CCNGG	2 cut(s)	83, 806
StyI	CCWWGG	2 cut(s)	864, 1125
TaaI	ACNGT	2 cut(s)	335, 756
TaiI	ACGT	4 cut(s)	592, 614, 675, 1043
TasI	AATT	5 cut(s)	12, 1067, 1151, 1174, 1195
TauI	GCSGC	3 cut(s)	277, 410, 918
TfiI	GAWTC	3 cut(s)	176, 653, 803
Tru1I	TTAA	5 cut(s)	185, 282, 572, 710, 1148
Tru9I	TTAA	5 cut(s)	185, 282, 572, 710, 1148
TscAI	CASTG	2 cut(s)	358, 1008
TseFI	GTSAC	1 cut(s)	70
TseI	GCWGC	1 cut(s)	900
Tsp45I	GTSAC	1 cut(s)	70
TspDTI	ATGAA	3 cut(s)	74, 248, 360
TspRI	CASTG	2 cut(s)	358, 1008
VneI	GTGCAC	1 cut(s)	226
XapI	RAATTY	1 cut(s)	1174
XbaI	TCTAGA	1 cut(s)	49
XceI	RCATGY	2 cut(s)	895, 1160
XmiI	GTMKAC	1 cut(s)	1186
XmnI	GAANNNNTTC	1 cut(s)	525
XspI	CTAG	3 cut(s)	50, 947, 1137
ZraI	GACGTC	1 cut(s)	1041

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.