Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0013868

Physical Location & Seq

Genomic Coordinates Forward (+)

3780706 .. 3781926

Gene Structure 1221 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1040300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1221 bp

ATGGACCAAGAAATCGACGTTCCCTCGTTTTTTCTCTGCCCCATTTCTCTAGAAATCATGAGGGATCCCGTCACAATCTCTACCGGGATCACATACGATCGGGAGAGCATAGAGAAATGGTTGTTCTCGAGCAAGAACAAGACTTGTCCCGTTACCAAACAAGTGGTTTCGGAGGATTCCGACATAACTCCAAATCACACTCTTCGCCGGTTGCTCCAAGCTTGGTGCACAATGAACGCTTCTCATGGCATCGAAAGGATCCCAACTCCAAAGCCGCCCGTCAACAAATCCCAGATCGCTAAGCTCCTCCGAGACGCCAAAAAGTCTCCAACCGGTCTGGCAAAATGCCTCCGCGAGCTTCGATCCATTTCGTCCGACAGCGAAGCCAACAAGAAAAGCATCGAAGCCGCCGGTGCAGGCGAGTTCCTTGCTTCAACGATAATCAACAACATCAAGAACGACAGAGAAAATGTCGAAAACGAAGAAGCTTTGAGCATCCTCCATAACCTAAATCTCAGCGAAGATGTTCTCAAAACCCTAATAGGCAAAGAGGGTGAGTTTGTTGAGGCCTTGATCAAACTCATGCAACGTGGGAGCTACGAGTCTCGAGCGTACGCTGTGATGTTGTTGAGATCAATTTTTGAGGTTGCTGATTCGACGAGGATGGCAAACGTAAAACCCGAAATTTTCGCCGAAGTTGTTCAGGTCTTAAATGATCAGATCTCACAACAGGCCTCAAAAGCCACATTGCAACTGTTGATCCAGCTCTGTCCGTGGGGACGTAACAGAATCAAAGCCGTTGAAGCAGGGGCGGTTATGGTCTTGATTGAGCTTCTTTTGGATACTTCATCTGATAGGAGAACCTTGGAAATGACACTCATGGTGCTTGACATGATATGCGGCTGCGCAGAAGGCCGCGCAGAGCTGCTGAACCACGGCGCAGGGCTCGCTGTGGTGTCTAAGAAGATAATGAGGGTCTCGAAGGTGGCCAGCGAGAGGGCAGTAAGGATTTTGTTCTCCATTTGCAAGTTTTCAGCGACGTCGAGTGTTTTGCAGGAGATGATGCAATTGGGTGTTGTGGCGAAGCTGTGCTTAGTTCTTCAAGTGGATAGTGGGAGCAAGACCAAGGATAAGGCTAGAGAGGTCTTAAAATTGCATGGAAGGACATGGAAGAATTCTACTTGTATACCAAGCAATTTGGTTTCTTCTTATCCATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010033 GO:0010035 GO:0010200 GO:0010243 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051865 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

U-box

Protein Analysis

407

Amino Acids

44.94

Weight (kDa)

8.59

Isoelectric Point (pI)

43.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
U-box	PF04564	7 - 79	3.2e-19	U-box domain
ARM_PUB	PF25598	98 - 404	5.7e-88	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013868)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35930
fragaria_vesca	FvH4_6g20400
malus_domestica	MD12G1040900.v1.1 MD14G1040300.v1.1
prunus_persica	Prupe.7G094000_v2.0.a1
pyrus_communis	pycom12g03520 pycom14g03420
rosa_chinensis	RchiOBHm_Chr3g0476101
rosa_laevigata	RLG00000023790
rosa_multiflora	Rmu_sc0000114.1_g000029
rosa_roxburghii	Rroxscaffold_6G00405470
rosa_rugosa	Rorug03G0152800
rosa_samantha	Rh3AG203800 Rh3BG234100 Rh3CG229400 Rh3DG229300
rosa_wichuraiana	Rw3G018520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1043
Acc16I	TGCGCA	1 cut(s)	907
AccI	GTMKAC	1 cut(s)	1186
AccII	CGCG	2 cut(s)	354, 918
AciI	CCGC	6 cut(s)	275, 352, 408, 812, 900, 916
AclWI	GGATC	7 cut(s)	59, 72, 95, 253, 266, 357, 754
AcoI	YGGCCR	1 cut(s)	987
AcsI	RAATTY	2 cut(s)	684, 1174
AcyI	GRCGYC	2 cut(s)	315, 1040
AfaI	GTAC	1 cut(s)	614
AfiI	CCNNNNNNNGG	1 cut(s)	996
AgeI	ACCGGT	1 cut(s)	332
AgsI	TTSAA	3 cut(s)	435, 803, 1103
AluBI	AGCT	9 cut(s)	221, 304, 358, 488, 597, 766, 832, 925, 1087
AluI	AGCT	9 cut(s)	221, 304, 358, 488, 597, 766, 832, 925, 1087
Alw21I	GWGCWC	1 cut(s)	230
Alw26I	GTCTC	4 cut(s)	306, 330, 609, 982
Alw44I	GTGCAC	1 cut(s)	226
AlwI	GGATC	7 cut(s)	59, 72, 95, 253, 266, 357, 754
Ama87I	CYCGRG	2 cut(s)	127, 606
AoxI	GGCC	4 cut(s)	567, 732, 913, 987
ApaLI	GTGCAC	1 cut(s)	226
ApeKI	GCWGC	2 cut(s)	903, 925
ApoI	RAATTY	2 cut(s)	684, 1174
AsiGI	ACCGGT	1 cut(s)	332
Asp700I	GAANNNNTTC	2 cut(s)	525, 699
AspLEI	GCGC	3 cut(s)	908, 920, 941
AspS9I	GGNCC	1 cut(s)	4
AsuC2I	CCSGG	1 cut(s)	85
AsuHPI	GGTGA	1 cut(s)	566
AvaI	CYCGRG	2 cut(s)	127, 606
AvaII	GGWCC	1 cut(s)	4
BaeGI	GKGCMC	1 cut(s)	230
BalI	TGGCCA	1 cut(s)	989
BamHI	GGATCC	2 cut(s)	64, 258
BanII	GRGCYC	1 cut(s)	948
Bbv12I	GWGCWC	1 cut(s)	230
BbvI	GCAGC	2 cut(s)	890, 912
BccI	CCATC	1 cut(s)	658
BceAI	ACGGC	2 cut(s)	782, 952
BcgI	CGANNNNNNTGC	4 cut(s)	410, 444, 1033, 1067
BciVI	GTATCC	1 cut(s)	835
BclI	TGATCA	2 cut(s)	573, 715
BcnI	CCSGG	1 cut(s)	85
BcoDI	GTCTC	4 cut(s)	306, 330, 609, 982
BfaI	CTAG	2 cut(s)	50, 1137
BfuI	GTATCC	1 cut(s)	835
BglII	AGATCT	1 cut(s)	720
BisI	GCNGC	6 cut(s)	275, 408, 901, 904, 916, 926
BlpI	GCTNAGC	1 cut(s)	300
BlsI	GCNGC	6 cut(s)	276, 409, 902, 905, 917, 927
Bme1390I	CCNGG	1 cut(s)	85
Bme18I	GGWCC	1 cut(s)	4
BmeT110I	CYCGRG	2 cut(s)	127, 606
BmgT120I	GGNCC	1 cut(s)	4
BmiI	GGNNCC	2 cut(s)	66, 260
BmrFI	CCNGG	1 cut(s)	85
BmsI	GCATC	4 cut(s)	258, 408, 504, 1053
Bpu1102I	GCTNAGC	1 cut(s)	300
BpuMI	CCSGG	1 cut(s)	85
BsaHI	GRCGYC	2 cut(s)	315, 1040
BsaI	GGTCTC	1 cut(s)	982
BsaJI	CCNNGG	4 cut(s)	773, 864, 934, 1125
BsaWI	WCCGGW	1 cut(s)	332
Bsc4I	CCNNNNNNNGG	1 cut(s)	996
Bse118I	RCCGGY	3 cut(s)	207, 332, 410
Bse3DI	GCAATG	1 cut(s)	746
BseDI	CCNNGG	4 cut(s)	773, 864, 934, 1125
BseGI	GGATG	2 cut(s)	495, 669
BseLI	CCNNNNNNNGG	1 cut(s)	996
BseMI	GCAATG	1 cut(s)	746
BseMII	CTCAG	1 cut(s)	529
BseRI	GAGGAG	1 cut(s)	296
BseSI	GKGCMC	1 cut(s)	230
BseXI	GCAGC	2 cut(s)	890, 912
BsgI	GTGCAG	1 cut(s)	435
Bsh1236I	CGCG	2 cut(s)	354, 918
Bsh1285I	CGRYCG	1 cut(s)	100
BshFI	GGCC	4 cut(s)	569, 734, 915, 989
BshTI	ACCGGT	1 cut(s)	332
BsiEI	CGRYCG	1 cut(s)	100
BsiHKAI	GWGCWC	1 cut(s)	230
BsiHKCI	CYCGRG	2 cut(s)	127, 606
BsiSI	CCGG	4 cut(s)	84, 208, 333, 411
BsiWI	CGTACG	1 cut(s)	612
BslFI	GGGAC	2 cut(s)	132, 792
BslI	CCNNNNNNNGG	1 cut(s)	996
BsmAI	GTCTC	4 cut(s)	306, 330, 609, 982
BsmBI	CGTCTC	1 cut(s)	306
BsmFI	GGGAC	2 cut(s)	132, 792
BsnI	GGCC	4 cut(s)	569, 734, 915, 989
Bso31I	GGTCTC	1 cut(s)	982
BsoBI	CYCGRG	2 cut(s)	127, 606
Bsp1286I	GDGCHC	2 cut(s)	230, 948
Bsp1720I	GCTNAGC	1 cut(s)	300
BspACI	CCGC	6 cut(s)	275, 352, 408, 812, 900, 916
BspANI	GGCC	4 cut(s)	569, 734, 915, 989
BspCNI	CTCAG	1 cut(s)	528
BspFNI	CGCG	2 cut(s)	354, 918
BspHI	TCATGA	1 cut(s)	57
BspLI	GGNNCC	2 cut(s)	66, 260
BspPI	GGATC	7 cut(s)	59, 72, 95, 253, 266, 357, 754
BspTNI	GGTCTC	1 cut(s)	982
BsrDI	GCAATG	1 cut(s)	746
BsrFI	RCCGGY	3 cut(s)	207, 332, 410
BssAI	RCCGGY	3 cut(s)	207, 332, 410
BssECI	CCNNGG	4 cut(s)	773, 864, 934, 1125
BssNAI	GTATAC	1 cut(s)	1187
BssNI	GRCGYC	2 cut(s)	315, 1040
BssT1I	CCWWGG	2 cut(s)	864, 1125
Bst1107I	GTATAC	1 cut(s)	1187
Bst4CI	ACNGT	1 cut(s)	756
Bst6I	CTCTTC	1 cut(s)	207
BstACI	GRCGYC	2 cut(s)	315, 1040
BstC8I	GCNNGC	4 cut(s)	356, 418, 948, 991
BstDEI	CTNAG	4 cut(s)	300, 515, 960, 1093
BstDSI	CCRYGG	2 cut(s)	773, 934
BstF5I	GGATG	2 cut(s)	495, 669
BstFNI	CGCG	2 cut(s)	354, 918
BstHHI	GCGC	3 cut(s)	908, 920, 941
BstMAI	GTCTC	4 cut(s)	306, 330, 609, 982
BstMCI	CGRYCG	1 cut(s)	100
BstMWI	GCNNNNNNNGC	5 cut(s)	413, 740, 803, 912, 947
BstSCI	CCNGG	1 cut(s)	83
BstSLI	GKGCMC	1 cut(s)	230
BstUI	CGCG	2 cut(s)	354, 918
BstV1I	GCAGC	2 cut(s)	890, 912
BstX2I	RGATCY	3 cut(s)	64, 258, 720
BstXI	CCANNNNNNTGG	1 cut(s)	163
BstYI	RGATCY	3 cut(s)	64, 258, 720
BstZ17I	GTATAC	1 cut(s)	1187
BsuI	GTATCC	1 cut(s)	835
BsuRI	GGCC	4 cut(s)	569, 734, 915, 989
BtgI	CCRYGG	2 cut(s)	773, 934
BtsCI	GGATG	2 cut(s)	495, 669
Cac8I	GCNNGC	4 cut(s)	356, 418, 948, 991
CciI	TCATGA	1 cut(s)	57
CfoI	GCGC	3 cut(s)	908, 920, 941
Cfr10I	RCCGGY	3 cut(s)	207, 332, 410
Cfr13I	GGNCC	1 cut(s)	4
CseI	GACGC	1 cut(s)	323
Csp6I	GTAC	1 cut(s)	613
CspAI	ACCGGT	1 cut(s)	332
CviAII	CATG	7 cut(s)	58, 245, 583, 880, 892, 1157, 1167
CviQI	GTAC	1 cut(s)	613
DdeI	CTNAG	4 cut(s)	300, 515, 960, 1093
EaeI	YGGCCR	1 cut(s)	987
Eam1104I	CTCTTC	1 cut(s)	207
EarI	CTCTTC	1 cut(s)	207
Eco130I	CCWWGG	2 cut(s)	864, 1125
Eco147I	AGGCCT	2 cut(s)	569, 734
Eco24I	GRGCYC	1 cut(s)	948
Eco31I	GGTCTC	1 cut(s)	982
Eco47I	GGWCC	1 cut(s)	4
Eco88I	CYCGRG	2 cut(s)	127, 606
EcoRI	GAATTC	1 cut(s)	1174
EcoT14I	CCWWGG	2 cut(s)	864, 1125
EcoT38I	GRGCYC	1 cut(s)	948
ErhI	CCWWGG	2 cut(s)	864, 1125
Esp3I	CGTCTC	1 cut(s)	306
FaeI	CATG	7 cut(s)	61, 248, 586, 883, 895, 1160, 1170
FalI	AAGNNNNNCTT	2 cut(s)	1076, 1108
FaqI	GGGAC	2 cut(s)	132, 792
FatI	CATG	7 cut(s)	57, 244, 582, 879, 891, 1156, 1166
FbaI	TGATCA	2 cut(s)	573, 715
FblI	GTMKAC	1 cut(s)	1186
Fnu4HI	GCNGC	6 cut(s)	275, 408, 901, 904, 916, 926
FokI	GGATG	2 cut(s)	482, 676
FriOI	GRGCYC	1 cut(s)	948
Fsp4HI	GCNGC	6 cut(s)	275, 408, 901, 904, 916, 926
FspBI	CTAG	2 cut(s)	50, 1137
FspI	TGCGCA	1 cut(s)	907
GlaI	GCGC	3 cut(s)	907, 919, 940
GluI	GCNGC	6 cut(s)	275, 408, 901, 904, 916, 926
HaeIII	GGCC	4 cut(s)	569, 734, 915, 989
HapII	CCGG	4 cut(s)	84, 208, 333, 411
HgaI	GACGC	1 cut(s)	323
HhaI	GCGC	3 cut(s)	908, 920, 941
Hin1I	GRCGYC	2 cut(s)	315, 1040
Hin1II	CATG	7 cut(s)	61, 248, 586, 883, 895, 1160, 1170
Hin6I	GCGC	3 cut(s)	906, 918, 939
HinP1I	GCGC	3 cut(s)	906, 918, 939
HincII	GTYRAC	1 cut(s)	283
HindII	GTYRAC	1 cut(s)	283
HindIII	AAGCTT	2 cut(s)	219, 486
HinfI	GANTC	4 cut(s)	176, 602, 653, 789
HpaII	CCGG	4 cut(s)	84, 208, 333, 411
HphI	GGTGA	1 cut(s)	566
Hpy166II	GTNNAC	3 cut(s)	228, 283, 1187
Hpy188I	TCNGA	6 cut(s)	172, 181, 311, 376, 720, 853
Hpy188III	TCNNGA	9 cut(s)	50, 58, 101, 127, 454, 606, 823, 979, 1218
Hpy8I	GTNNAC	3 cut(s)	228, 283, 1187
Hpy99I	CGWCG	4 cut(s)	20, 661, 1042, 1045
HpyAV	CCTTC	3 cut(s)	905, 976, 1155
HpyCH4III	ACNGT	1 cut(s)	756
HpyCH4IV	ACGT	5 cut(s)	18, 589, 672, 781, 1040
HpyCH4V	TGCA	8 cut(s)	228, 416, 586, 751, 1026, 1054, 1066, 1156
HpyF10VI	GCNNNNNNNGC	5 cut(s)	413, 740, 803, 912, 947
HpyF3I	CTNAG	4 cut(s)	300, 515, 960, 1093
HpySE526I	ACGT	5 cut(s)	18, 589, 672, 781, 1040
Hsp92I	GRCGYC	2 cut(s)	315, 1040
Hsp92II	CATG	7 cut(s)	61, 248, 586, 883, 895, 1160, 1170
HspAI	GCGC	3 cut(s)	906, 918, 939
Ksp22I	TGATCA	2 cut(s)	573, 715
LmnI	GCTCC	4 cut(s)	219, 309, 594, 1116
Lsp1109I	GCAGC	2 cut(s)	890, 912
LweI	GCATC	4 cut(s)	258, 408, 504, 1053
MaeI	CTAG	2 cut(s)	50, 1137
MaeII	ACGT	5 cut(s)	18, 589, 672, 781, 1040
MaeIII	GTNAC	3 cut(s)	70, 151, 782
MboII	GAAGA	7 cut(s)	194, 494, 533, 976, 1091, 1183, 1197
MfeI	CAATTG	1 cut(s)	1067
MflI	RGATCY	3 cut(s)	64, 258, 720
MhlI	GDGCHC	2 cut(s)	230, 948
MlsI	TGGCCA	1 cut(s)	989
MluCI	AATT	6 cut(s)	636, 684, 1067, 1151, 1174, 1195
MluNI	TGGCCA	1 cut(s)	989
MlyI	GAGTC	1 cut(s)	611
MmeI	TCCRAC	3 cut(s)	204, 353, 399
Mox20I	TGGCCA	1 cut(s)	989
MroXI	GAANNNNTTC	2 cut(s)	525, 699
MscI	TGGCCA	1 cut(s)	989
MseI	TTAA	2 cut(s)	710, 1148
Msp20I	TGGCCA	1 cut(s)	989
MspI	CCGG	4 cut(s)	84, 208, 333, 411
MspR9I	CCNGG	1 cut(s)	85
MunI	CAATTG	1 cut(s)	1067
MvnI	CGCG	2 cut(s)	354, 918
MwoI	GCNNNNNNNGC	5 cut(s)	413, 740, 803, 912, 947
NciI	CCSGG	1 cut(s)	85
NlaIII	CATG	7 cut(s)	61, 248, 586, 883, 895, 1160, 1170
NlaIV	GGNNCC	2 cut(s)	66, 260
NmuCI	GTSAC	1 cut(s)	70
NsbI	TGCGCA	1 cut(s)	907
PaeR7I	CTCGAG	2 cut(s)	127, 606
PagI	TCATGA	1 cut(s)	57
PceI	AGGCCT	2 cut(s)	569, 734
PcsI	WCGNNNNNNNCGW	1 cut(s)	678
PdmI	GAANNNNTTC	2 cut(s)	525, 699
PfeI	GAWTC	3 cut(s)	176, 653, 789
Pfl23II	CGTACG	1 cut(s)	612
PinAI	ACCGGT	1 cut(s)	332
PkrI	GCNGC	6 cut(s)	276, 409, 902, 905, 917, 927
Ple19I	CGATCG	1 cut(s)	100
PleI	GAGTC	1 cut(s)	610
PpsI	GAGTC	1 cut(s)	610
PspLI	CGTACG	1 cut(s)	612
PspN4I	GGNNCC	2 cut(s)	66, 260
PspPI	GGNCC	1 cut(s)	4
PsuI	RGATCY	3 cut(s)	64, 258, 720
PvuI	CGATCG	1 cut(s)	100
RsaI	GTAC	1 cut(s)	614
RsaNI	GTAC	1 cut(s)	613
SaqAI	TTAA	2 cut(s)	710, 1148
SatI	GCNGC	6 cut(s)	275, 408, 901, 904, 916, 926
Sau96I	GGNCC	1 cut(s)	4
SchI	GAGTC	1 cut(s)	611
ScrFI	CCNGG	1 cut(s)	85
SduI	GDGCHC	2 cut(s)	230, 948
SfaNI	GCATC	4 cut(s)	258, 408, 504, 1053
Sfr274I	CTCGAG	2 cut(s)	127, 606
SgrAI	CRCCGGYG	1 cut(s)	410
SinI	GGWCC	1 cut(s)	4
SlaI	CTCGAG	2 cut(s)	127, 606
SmlI	CTYRAG	2 cut(s)	127, 606
SmoI	CTYRAG	2 cut(s)	127, 606
Sse9I	AATT	6 cut(s)	636, 684, 1067, 1151, 1174, 1195
SseBI	AGGCCT	2 cut(s)	569, 734
SsiI	CCGC	6 cut(s)	275, 352, 408, 812, 900, 916
SspMI	CTAG	2 cut(s)	50, 1137
StuI	AGGCCT	2 cut(s)	569, 734
StyD4I	CCNGG	1 cut(s)	83
StyI	CCWWGG	2 cut(s)	864, 1125
TaaI	ACNGT	1 cut(s)	756
TaiI	ACGT	5 cut(s)	21, 592, 675, 784, 1043
TasI	AATT	6 cut(s)	636, 684, 1067, 1151, 1174, 1195
TauI	GCSGC	4 cut(s)	277, 410, 903, 918
TfiI	GAWTC	3 cut(s)	176, 653, 789
Tru1I	TTAA	2 cut(s)	710, 1148
Tru9I	TTAA	2 cut(s)	710, 1148
TseFI	GTSAC	1 cut(s)	70
TseI	GCWGC	2 cut(s)	903, 925
Tsp45I	GTSAC	1 cut(s)	70
TspDTI	ATGAA	2 cut(s)	248, 837
TspGWI	ACGGA	1 cut(s)	762
VneI	GTGCAC	1 cut(s)	226
VpaK11BI	GGWCC	1 cut(s)	4
XapI	RAATTY	2 cut(s)	684, 1174
XbaI	TCTAGA	1 cut(s)	49
XhoI	CTCGAG	2 cut(s)	127, 606
XmiI	GTMKAC	1 cut(s)	1186
XmnI	GAANNNNTTC	2 cut(s)	525, 699
XspI	CTAG	2 cut(s)	50, 1137
ZraI	GACGTC	1 cut(s)	1041

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.