MD12G1057000.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011995
Physical Location & Seq
Reverse (-)
6524989 .. 6528345
3357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1057000.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGTTTTCGTGGCTGGCAAGGATAGCGTGTACGTGTTGGAGGCCATTGAGGAGATATGCCCGTATGAACAAGGATGATGTCGAGGACTCGTCGTTGGAGGACCCTTTGCTGTGGTGCAGAAGCCTCGACAGGCACAGCTATGGAGAATTCTCCTTCGCTGTCGTGCAGGCCAATGAAGTTATTGAGGATCACAGCCAAGTGGAGACCGGCCGTGACGCCACCTTCGTCGGTGTTTATGACGGACATGGTGGACCTGATGCTTCTCGCTTCATTAATGACCATCTCTTTCTTCATCTCATGAGACATGCTCGAGAAAAGGGAACCATATCTGAAGATACCCTTAGAAGTGCCTTTTCTTCAACTGAGGATGGGTTCCTTACTCTTGTGCGTAGGACATGTGGAATAAAGCCATTAATGGCAGCAATTGGGTCTTGTTGTCTGGTTGGAGTTATCTGGAGGGGGACATTGTATGTTGCTAATCTAGGAGACTCCCGAGCAGTCATTGGTTCTGTAGGTAGATCGAATAAGATAGGTGCCGAACAGCTAAATAGGGAGCACAATGCAAGTATGGAGGAGGTTAGACAAGAACTCAGGTCTTTGCATCCAAATGATTCACATATTGTAGTTATGAAGCAGGGAGTTTGGCGTATCAAAGGCATCATACAGGTATCCAGATCTATCGGTGATGCATACTTAAAGAGGCCAGAATTTTCTCTTGATCCCACGTTTCCAAGGTTCCACCTTCCTGAACCTCTTGGCCGGCCTGTGCTAACAGCAGAACCATCTGTATGTGCTAGAGTTCTACAGCCCAATGATAAATTTGTAATATTCGCATCCGATGGGCTATGGGAGCACATGACAAATCAGGAAGCTGTGGAGATTGTACACAATAATCCTCGAGCAGGAATTGCGAAAAGACTTCTTAAAACAGCTCTGAGTGAGGCAGCTAGAAAAAGGGAGATGAGACACGATGACCTTAAGAAGGTTGAAAAGGGAGTTAGGCGGTTCTTTCACGATGACATTACGATTGTTGTCATTTTTATAGATCACGATTTACTGGGGAAGAACCAACCCGCGCCTGAGCTGTCAGTAAGAGGGTTCATTGACACTGTTGGACCGTCAAGGTTTAACATGTTCAAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

382

Amino Acids

43.11

Weight (kDa)

7.71

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 71 - 317 8.9e-37 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011995)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06750 AT5G06750 AT5G06750
fragaria_vesca FvH4_6g22310
malus_domestica MD12G1057000.v1.1 MD14G1057600.v1.1
prunus_persica Prupe.7G077100_v2.0.a1
pyrus_communis pycom12g05090 pycom14g04670
rosa_chinensis RchiOBHm_Chr3g0479661
rosa_laevigata RLG00000023549
rosa_multiflora Rmu_sc0003755.1_g000003
rosa_roxburghii Rroxscaffold_6G00402080
rosa_rugosa Rorug03G0177300
rosa_samantha Rh3AG228000 Rh3BG261200 Rh3CG258300 Rh3DG254500
rosa_wichuraiana Rw3G020400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 533
AccII CGCG 1 cut(s) 1076
AciI CCGC 2 cut(s) 1003, 1074
AclWI GGATC 2 cut(s) 195, 713
AcoI YGGCCR 2 cut(s) 208, 757
AcsI RAATTY 3 cut(s) 146, 707, 818
AcuI CTGAAG 1 cut(s) 351
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 2 cut(s) 31, 885
AfiI CCNNNNNNNGG 2 cut(s) 902, 982
AflII CTTAAG 1 cut(s) 977
AflIII ACRYGT 3 cut(s) 32, 395, 1131
AgsI TTSAA 3 cut(s) 360, 989, 1138
AluBI AGCT 6 cut(s) 138, 544, 872, 932, 947, 1084
AluI AGCT 6 cut(s) 138, 544, 872, 932, 947, 1084
Alw21I GWGCWC 2 cut(s) 558, 855
Alw26I GTCTC 4 cut(s) 197, 295, 480, 958
AlwI GGATC 2 cut(s) 195, 713
Ama87I CYCGRG 3 cut(s) 309, 492, 897
AoxI GGCC 6 cut(s) 41, 168, 208, 701, 757, 761
ApeKI GCWGC 2 cut(s) 419, 944
ApoI RAATTY 3 cut(s) 146, 707, 818
ArsI GACNNNNNNTTYG 2 cut(s) 206, 238
AseI ATTAAT 2 cut(s) 273, 413
AspLEI GCGC 1 cut(s) 1078
AspS9I GGNCC 3 cut(s) 100, 251, 1115
AsuHPI GGTGA 1 cut(s) 695
AvaI CYCGRG 3 cut(s) 309, 492, 897
AvaII GGWCC 3 cut(s) 100, 251, 1115
BanI GGYRCC 1 cut(s) 533
Bbv12I GWGCWC 2 cut(s) 558, 855
BbvI GCAGC 2 cut(s) 431, 956
BccI CCATC 4 cut(s) 288, 362, 790, 833
BceAI ACGGC 1 cut(s) 195
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciVI GTATCC 1 cut(s) 679
BcoDI GTCTC 4 cut(s) 197, 295, 480, 958
BfaI CTAG 3 cut(s) 482, 795, 948
BfmI CTRYAG 2 cut(s) 510, 803
BfrI CTTAAG 1 cut(s) 977
BfuI GTATCC 1 cut(s) 679
BglII AGATCT 1 cut(s) 674
BisI GCNGC 2 cut(s) 420, 945
BlsI GCNGC 2 cut(s) 421, 946
Bme18I GGWCC 3 cut(s) 100, 251, 1115
BmeT110I CYCGRG 3 cut(s) 309, 492, 897
BmgT120I GGNCC 3 cut(s) 100, 251, 1115
BmiI GGNNCC 5 cut(s) 102, 322, 374, 535, 737
BmrI ACTGGG 1 cut(s) 1067
BmsI GCATC 5 cut(s) 247, 610, 666, 676, 842
BmuI ACTGGG 1 cut(s) 1067
BplI GAGNNNNNCTC 2 cut(s) 292, 324
BpmI CTGGAG 1 cut(s) 475
Bpu10I CCTNAGC 1 cut(s) 1080
BsaAI YACGTR 1 cut(s) 33
BsaHI GRCGYC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 731
Bsc4I CCNNNNNNNGG 2 cut(s) 902, 982
Bse118I RCCGGY 2 cut(s) 206, 759
Bse1I ACTGG 1 cut(s) 1062
BseDI CCNNGG 1 cut(s) 731
BseGI GGATG 4 cut(s) 79, 373, 601, 833
BseLI CCNNNNNNNGG 2 cut(s) 902, 982
BseMII CTCAG 4 cut(s) 354, 604, 926, 1071
BseNI ACTGG 1 cut(s) 1062
BseRI GAGGAG 2 cut(s) 64, 587
BseX3I CGGCCG 1 cut(s) 208
BseXI GCAGC 2 cut(s) 431, 956
BsgI GTGCAG 2 cut(s) 136, 185
Bsh1236I CGCG 1 cut(s) 1076
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 6 cut(s) 43, 170, 210, 703, 759, 763
BshNI GGYRCC 1 cut(s) 533
BsiEI CGRYCG 1 cut(s) 211
BsiHKAI GWGCWC 2 cut(s) 558, 855
BsiHKCI CYCGRG 3 cut(s) 309, 492, 897
BsiSI CCGG 2 cut(s) 207, 760
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 2 cut(s) 902, 982
BsmAI GTCTC 4 cut(s) 197, 295, 480, 958
BsmFI GGGAC 1 cut(s) 475
BsnI GGCC 6 cut(s) 43, 170, 210, 703, 759, 763
Bso31I GGTCTC 1 cut(s) 197
BsoBI CYCGRG 3 cut(s) 309, 492, 897
Bsp1286I GDGCHC 2 cut(s) 558, 855
Bsp1407I TGTACA 1 cut(s) 883
Bsp143I GATC 5 cut(s) 187, 518, 674, 718, 1045
BspACI CCGC 2 cut(s) 1003, 1074
BspANI GGCC 6 cut(s) 43, 170, 210, 703, 759, 763
BspCNI CTCAG 4 cut(s) 355, 603, 927, 1072
BspFNI CGCG 1 cut(s) 1076
BspHI TCATGA 1 cut(s) 297
BspLI GGNNCC 5 cut(s) 102, 322, 374, 535, 737
BspPI GGATC 2 cut(s) 195, 713
BspT107I GGYRCC 1 cut(s) 533
BspTI CTTAAG 1 cut(s) 977
BspTNI GGTCTC 1 cut(s) 197
BsrFI RCCGGY 2 cut(s) 206, 759
BsrGI TGTACA 1 cut(s) 883
BsrI ACTGG 1 cut(s) 1062
BssAI RCCGGY 2 cut(s) 206, 759
BssECI CCNNGG 1 cut(s) 731
BssMI GATC 5 cut(s) 187, 518, 674, 718, 1045
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 731
Bst4CI ACNGT 2 cut(s) 1111, 1119
BstACI GRCGYC 1 cut(s) 216
BstAFI CTTAAG 1 cut(s) 977
BstAPI GCANNNNNTGC 1 cut(s) 908
BstAUI TGTACA 1 cut(s) 883
BstBAI YACGTR 1 cut(s) 33
BstC8I GCNNGC 3 cut(s) 15, 168, 761
BstDEI CTNAG 5 cut(s) 341, 363, 590, 935, 1080
BstENI CCTNNNNNAGG 2 cut(s) 900, 980
BstF5I GGATG 4 cut(s) 79, 373, 601, 833
BstFNI CGCG 1 cut(s) 1076
BstHHI GCGC 1 cut(s) 1078
BstKTI GATC 5 cut(s) 190, 521, 677, 721, 1048
BstMAI GTCTC 4 cut(s) 197, 295, 480, 958
BstMBI GATC 5 cut(s) 187, 518, 674, 718, 1045
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 3 cut(s) 23, 850, 908
BstNSI RCATGY 3 cut(s) 308, 399, 1135
BstSFI CTRYAG 2 cut(s) 510, 803
BstUI CGCG 1 cut(s) 1076
BstV1I GCAGC 2 cut(s) 431, 956
BstX2I RGATCY 1 cut(s) 674
BstYI RGATCY 1 cut(s) 674
BstZI CGGCCG 1 cut(s) 208
BsuI GTATCC 1 cut(s) 679
BsuRI GGCC 6 cut(s) 43, 170, 210, 703, 759, 763
BtsCI GGATG 4 cut(s) 79, 373, 601, 833
BtsIMutI CAGTG 1 cut(s) 1107
Cac8I GCNNGC 3 cut(s) 15, 168, 761
CciI TCATGA 1 cut(s) 297
CfoI GCGC 1 cut(s) 1078
Cfr10I RCCGGY 2 cut(s) 206, 759
Cfr13I GGNCC 3 cut(s) 100, 251, 1115
CseI GACGC 1 cut(s) 224
Csp6I GTAC 2 cut(s) 30, 884
CviAII CATG 6 cut(s) 245, 298, 305, 396, 856, 1132
CviQI GTAC 2 cut(s) 30, 884
DdeI CTNAG 5 cut(s) 341, 363, 590, 935, 1080
DpnI GATC 5 cut(s) 189, 520, 676, 720, 1047
DpnII GATC 5 cut(s) 187, 518, 674, 718, 1045
EaeI YGGCCR 2 cut(s) 208, 757
EagI CGGCCG 1 cut(s) 208
EclXI CGGCCG 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 731
Eco31I GGTCTC 1 cut(s) 197
Eco47I GGWCC 3 cut(s) 100, 251, 1115
Eco52I CGGCCG 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 351
Eco88I CYCGRG 3 cut(s) 309, 492, 897
EcoNI CCTNNNNNAGG 2 cut(s) 900, 980
EcoO109I RGGNCCY 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 731
EcoT22I ATGCAT 1 cut(s) 691
ErhI CCWWGG 1 cut(s) 731
FaeI CATG 6 cut(s) 248, 301, 308, 399, 859, 1135
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FaqI GGGAC 1 cut(s) 475
FatI CATG 6 cut(s) 244, 297, 304, 395, 855, 1131
FauI CCCGC 1 cut(s) 1081
Fnu4HI GCNGC 2 cut(s) 420, 945
FokI GGATG 4 cut(s) 86, 380, 588, 820
FseI GGCCGGCC 1 cut(s) 763
Fsp4HI GCNGC 2 cut(s) 420, 945
FspBI CTAG 3 cut(s) 482, 795, 948
GlaI GCGC 1 cut(s) 1077
GluI GCNGC 2 cut(s) 420, 945
GsuI CTGGAG 1 cut(s) 475
HaeIII GGCC 6 cut(s) 43, 170, 210, 703, 759, 763
HapII CCGG 2 cut(s) 207, 760
HgaI GACGC 1 cut(s) 224
HhaI GCGC 1 cut(s) 1078
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 6 cut(s) 248, 301, 308, 399, 859, 1135
Hin6I GCGC 1 cut(s) 1076
HinP1I GCGC 1 cut(s) 1076
HinfI GANTC 3 cut(s) 86, 488, 611
HpaII CCGG 2 cut(s) 207, 760
HphI GGTGA 1 cut(s) 695
Hpy166II GTNNAC 3 cut(s) 30, 251, 886
Hpy188I TCNGA 3 cut(s) 331, 838, 936
Hpy8I GTNNAC 3 cut(s) 30, 251, 886
Hpy99I CGWCG 2 cut(s) 94, 230
HpyAV CCTTC 4 cut(s) 163, 232, 752, 976
HpyCH4III ACNGT 2 cut(s) 1111, 1119
HpyCH4IV ACGT 2 cut(s) 32, 725
HpyCH4V TGCA 5 cut(s) 117, 166, 563, 601, 689
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 850, 908
HpyF3I CTNAG 5 cut(s) 341, 363, 590, 935, 1080
HpySE526I ACGT 2 cut(s) 32, 725
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 6 cut(s) 248, 301, 308, 399, 859, 1135
HspAI GCGC 1 cut(s) 1076
KroI GCCGGC 1 cut(s) 759
KroNI GCCGGC 1 cut(s) 761
Kzo9I GATC 5 cut(s) 187, 518, 674, 718, 1045
LmnI GCTCC 2 cut(s) 553, 850
Lsp1109I GCAGC 2 cut(s) 431, 956
LweI GCATC 5 cut(s) 247, 610, 666, 676, 842
MaeI CTAG 3 cut(s) 482, 795, 948
MaeII ACGT 2 cut(s) 32, 725
MaeIII GTNAC 1 cut(s) 212
MalI GATC 5 cut(s) 189, 520, 676, 720, 1047
MboI GATC 5 cut(s) 187, 518, 674, 718, 1045
MboII GAAGA 4 cut(s) 281, 344, 348, 1075
MfeI CAATTG 1 cut(s) 423
MflI RGATCY 1 cut(s) 674
MhlI GDGCHC 2 cut(s) 558, 855
MluCI AATT 5 cut(s) 146, 423, 707, 818, 906
MlyI GAGTC 2 cut(s) 80, 482
MmeI TCCRAC 4 cut(s) 17, 75, 424, 1093
Mph1103I ATGCAT 1 cut(s) 691
MroNI GCCGGC 1 cut(s) 759
MseI TTAA 6 cut(s) 273, 413, 695, 924, 978, 1128
MslI CAYNNNNRTG 3 cut(s) 138, 606, 787
MspCI CTTAAG 1 cut(s) 977
MspI CCGG 2 cut(s) 207, 760
MunI CAATTG 1 cut(s) 423
MvnI CGCG 1 cut(s) 1076
MwoI GCNNNNNNNGC 3 cut(s) 23, 850, 908
NaeI GCCGGC 1 cut(s) 761
NdeII GATC 5 cut(s) 187, 518, 674, 718, 1045
NgoMIV GCCGGC 1 cut(s) 759
NlaIII CATG 6 cut(s) 248, 301, 308, 399, 859, 1135
NlaIV GGNNCC 5 cut(s) 102, 322, 374, 535, 737
NmuCI GTSAC 1 cut(s) 212
NsiI ATGCAT 1 cut(s) 691
NspI RCATGY 3 cut(s) 308, 399, 1135
PaeR7I CTCGAG 2 cut(s) 309, 897
PagI TCATGA 1 cut(s) 297
PciI ACATGT 2 cut(s) 395, 1131
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdiI GCCGGC 1 cut(s) 761
PfeI GAWTC 1 cut(s) 611
PkrI GCNGC 2 cut(s) 421, 946
PleI GAGTC 2 cut(s) 80, 482
PpsI GAGTC 2 cut(s) 80, 482
Ppu21I YACGTR 1 cut(s) 33
PpuMI RGGWCCY 1 cut(s) 100
PscI ACATGT 2 cut(s) 395, 1131
PshBI ATTAAT 2 cut(s) 273, 413
Psp5II RGGWCCY 1 cut(s) 100
PspN4I GGNNCC 5 cut(s) 102, 322, 374, 535, 737
PspPI GGNCC 3 cut(s) 100, 251, 1115
PspPPI RGGWCCY 1 cut(s) 100
PspXI VCTCGAGB 1 cut(s) 897
PsuI RGATCY 1 cut(s) 674
RigI GGCCGGCC 1 cut(s) 763
RsaI GTAC 2 cut(s) 31, 885
RsaNI GTAC 2 cut(s) 30, 884
RseI CAYNNNNRTG 3 cut(s) 138, 606, 787
SaqAI TTAA 6 cut(s) 273, 413, 695, 924, 978, 1128
SatI GCNGC 2 cut(s) 420, 945
Sau3AI GATC 5 cut(s) 187, 518, 674, 718, 1045
Sau96I GGNCC 3 cut(s) 100, 251, 1115
SchI GAGTC 2 cut(s) 80, 482
SduI GDGCHC 2 cut(s) 558, 855
SfaNI GCATC 5 cut(s) 247, 610, 666, 676, 842
SfcI CTRYAG 2 cut(s) 510, 803
Sfr274I CTCGAG 2 cut(s) 309, 897
SinI GGWCC 3 cut(s) 100, 251, 1115
SlaI CTCGAG 2 cut(s) 309, 897
SmiMI CAYNNNNRTG 3 cut(s) 138, 606, 787
SmlI CTYRAG 3 cut(s) 309, 897, 977
SmoI CTYRAG 3 cut(s) 309, 897, 977
Sse9I AATT 5 cut(s) 146, 423, 707, 818, 906
SsiI CCGC 2 cut(s) 1003, 1074
SspI AATATT 1 cut(s) 828
SspMI CTAG 3 cut(s) 482, 795, 948
StyI CCWWGG 1 cut(s) 731
TaaI ACNGT 2 cut(s) 1111, 1119
TaiI ACGT 2 cut(s) 35, 728
TaqI TCGA 5 cut(s) 81, 126, 310, 521, 898
TasI AATT 5 cut(s) 146, 423, 707, 818, 906
TatI WGTACW 1 cut(s) 883
TfiI GAWTC 1 cut(s) 611
Tru1I TTAA 6 cut(s) 273, 413, 695, 924, 978, 1128
Tru9I TTAA 6 cut(s) 273, 413, 695, 924, 978, 1128
TscAI CASTG 1 cut(s) 1114
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 2 cut(s) 419, 944
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 6 cut(s) 80, 189, 259, 281, 644, 1090
TspGWI ACGGA 1 cut(s) 255
TspRI CASTG 1 cut(s) 1114
Vha464I CTTAAG 1 cut(s) 977
VpaK11BI GGWCC 3 cut(s) 100, 251, 1115
VspI ATTAAT 2 cut(s) 273, 413
XagI CCTNNNNNAGG 2 cut(s) 900, 980
XapI RAATTY 3 cut(s) 146, 707, 818
XceI RCATGY 3 cut(s) 308, 399, 1135
XhoI CTCGAG 2 cut(s) 309, 897
XspI CTAG 3 cut(s) 482, 795, 948
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.