Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006985

Physical Location & Seq

Genomic Coordinates Forward (+)

7128710 .. 7132475

Gene Structure 3766 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1061300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2325 bp

ATGCAGAGACCTCCCCCGGAAGACTTTGCTTTGAAGGAGACCAAACCCCACCTCGGTGGGGGGAGGATCTCCGGTGACAAGCTAACGAGCACGTATGACCTCGTTGAGCAGATGCAGTACCTCTATGTCCGGGTTGTTAAGGCGAAGGACTTGCCCACGAAAGATGTTACTGGTAGCTGTGACCCTTATGTCGAAGTTAAGCTTGGGAATTACAAAGGCGCGACTCGACATTTTGAGAAGAAGTCAAATCCGGAGTGGAACCAGGTTTTCGCATTCTCGAAAGATCGTATTCAGGCGACGGTTCTTGAGGTCATTGTGAAGGACAAGGATCTTATGAAGGATGATTTCATGGGCCGTGTTTCTTTTGATCTGAACGAGGTTCCAAAACGTGTTCCTCCCGATAGTCCTCTGGCACCACAGTGGTATAGATTGGAAGATCATAAGGGGAACAAGGCAAGGGGAGAGCTGATGTTGGCTGTATGGATGGGCACCCAAGCTGATGAAGCTTTTCCTGAAGCATGGCATTCCGATGCTGCCACAATTAGCGGAGCTGACAGTCTTGCAAATATCCGATCAAAGGTGTATCTCTCTCCTAAGCTTTGGTATTTGAGGGTTAATGTGATTGAAGCTCAGGATTTGATGCCGAGTGATAAGGGTCGGTATCCTGAAGTTTATGTGAAAGCTATACTTGGAACTCAAGCTTTGAGAACTAGGATTTCTCCAAGCAGGAGCATAAATCCTATGTGGAACGAAGATTTAATGTTTGTTGCATCAGAACCATTTGAGGAGCCCTTGATTTTGAGTGTGGAGGACAGAATTGCACCTAACAAAGATGAAGTTTTGGGGAGATGTGCAATTCCTCTGCAGTATGTGCCCCGAAGGTATGATCATAAACCTGTTAACACTAGCTGGCACAATCTTGAAAAGCATGTTATTGTAGAAGGGGAAAAGAAGAAGGAAATCAAGTTTGCAAGCAGGATTCATATGAGGATCTGTCTGGAAGGTGGTTACCATGTCCTTGATGAATCAACACACTATAGTAGTGATCTTCGACCGACAGCAAAACCGTTGTGGAAGTCCAGCATTGGGATCCTTGAAGTGGGAATTCTGAATGCTCAGGGGTTGATGCCAATGAAGACCAAAGATGGGAGGGGTACGACAGATGCGTATTGTGTGGCGAAATATGGGCAGAAATGGGTTCGAACAAGAACCATTATTGATAGCTTTATGCCCAGGTGGAATGAGCAGTACACTTGGGAAGTGTTTGATCCTTGTACTGTCATAACAATTGGGGTATTTGATAACTGCCATCTGCATGGGGGAGAAAAGGCTGGAGGGGCCAAGGATGCAAGAATTGGGAAGGTAAGGATTCGTCTCTCCACCCTCGAGACTGATCGGGTTTACACACACTCGTACCCGCTTTTGGTTCTGCACCCAAATGGTGTAAAGAAGATGGGTGAAATTCATATGGCCGTGCGGTTCACTTGCTCTTCTCTGCTTAACATGATGCACATGTACTCACAGCCCCTGCTGCCAAAAATGCACTACATTCATCCATTGACTGTAAGTCAGCTTGATAGTTTGAGGCACCAGGCCACTCAGATTGTATCGATAAGGCTGAGCCGCGCTGAGCCACCGTTGAGGAAAGAGGTGGTCGAATATATGCTGGATGTGGGCTCCCACATGTGGAGTATGAGAAGAAGCAAAGCTAACTTTTTCAGGATTATGAATGTTTTTGGTGGGATAATTGCTGTTGGAAAATGGTTTGATCAGATCTGCAACTGGAAAAACCCCATTACTACCGTACTCATTCACATCCTCTTTATTATTCTGGTCATGTACCCGGAGCTCATATTGCCTACAATTTTCCTCTACCTCTTCTTGATTGGAGTTTGGTACTATAGATGGAGGCCAAGGCACCCTCCTCACATGGACACTCGTCTGTCTCATGCAGATTCTGCACATCCCGATGAACTTGATGAAGAATTTGATACATTCCCTACTTCGCGGCCTTCTGACATAGTGAGGATGAGATATGATAGGTTGAGGAGTATTGCAGGGAGAATCCAGACAGTGGTTGGCGACTTGGCTACTCAAGGGGAGAGGCTGCAATCTTTGCTGAGCTGGAGAGACCCGAGAGCTACTGCACTGTTTGTGCTTTTCTGTCTGATTGCAGCGATTGTTCTGTATGTTACTCCGTTCCAGGTTGTGGCTCTTCTTGCTGGATTTTATGTGCTGAGACACCCAAGATTCCGTCATAAGCTTCCTTCTGTGCCGCTGAATTTCTTCAGGAGGTTGCCGGCTAGAACTGACTGCATGCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023052 GO:0031224 GO:0031974 GO:0031981 GO:0035556 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 PRT_C

Protein Analysis

775

Amino Acids

89.04

Weight (kDa)

9.19

Isoelectric Point (pI)

47.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	40 - 143	5.7e-24	C2 domain
C2	PF00168	201 - 306	3e-19	C2 domain
C2	PF00168	363 - 474	5.2e-20	C2 domain
Pex24p	PF06398	591 - 754	3e-07	Integral peroxisomal membrane peroxin
PRT_C	PF08372	619 - 774	4e-83	Plant phosphoribosyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006985)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51570 AT3G57880 AT3G57880 AT3G57880 AT3G57880
fragaria_vesca	FvH4_6g22730 FvH4_6g22730 FvH4_6g22730
malus_domestica	MD12G1061300.v1.1 MD14G1061300.v1.1
prunus_persica	Prupe.7G073400_v2.0.a1
pyrus_communis	pycom12g05420 pycom14g04960
rosa_chinensis	RchiOBHm_Chr3g0480351
rosa_laevigata	RLG00000023491
rosa_multiflora	Rmu_sc0004090.1_g000007
rosa_roxburghii	Rroxscaffold_6G00401530
rosa_rugosa	Rorug03G0182800 Rorug03G0182900 Rorug03G0182900
rosa_samantha	Rh3AG234600 Rh3BG266800 Rh3CG264400 Rh3DG260100
rosa_wichuraiana	Rw3G021050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	188
AccB1I	GGYRCC	4 cut(s)	412, 488, 1587, 1917
AccB7I	CCANNNNNTGG	2 cut(s)	2074, 2209
AccII	CGCG	3 cut(s)	221, 1626, 2008
AccIII	TCCGGA	1 cut(s)	250
AciI	CCGC	6 cut(s)	546, 1418, 1477, 1624, 2008, 2276
AclWI	GGATC	6 cut(s)	74, 336, 998, 1084, 1097, 1262
AcoI	YGGCCR	1 cut(s)	1470
AcsI	RAATTY	4 cut(s)	1104, 1461, 1985, 2281
AcuI	CTGAAG	3 cut(s)	534, 687, 2272
AfaI	GTAC	9 cut(s)	119, 1156, 1250, 1276, 1415, 1517, 1806, 1841, 1898
AflIII	ACRYGT	3 cut(s)	388, 1512, 1683
AgsI	TTSAA	4 cut(s)	34, 626, 923, 1097
AhdI	GACNNNNNGTC	1 cut(s)	1566
AjnI	CCWGG	4 cut(s)	261, 1232, 1590, 2202
AjuI	GAANNNNNNNTTGG	2 cut(s)	186, 218
AleI	CACNNNNGTG	2 cut(s)	54, 418
Alw21I	GWGCWC	2 cut(s)	92, 1851
Alw26I	GTCTC	6 cut(s)	32, 1379, 1382, 1950, 2124, 2233
AlwI	GGATC	6 cut(s)	74, 336, 998, 1084, 1097, 1262
AlwNI	CAGNNNCTG	2 cut(s)	1528, 1958
Ama87I	CYCGRG	2 cut(s)	1385, 2134
Aor13HI	TCCGGA	1 cut(s)	250
AoxI	GGCC	6 cut(s)	352, 1338, 1470, 1593, 1910, 2009
ApeKI	GCWGC	4 cut(s)	533, 1531, 2107, 2174
ApoI	RAATTY	4 cut(s)	1104, 1461, 1985, 2281
Asp700I	GAANNNNTTC	2 cut(s)	507, 2285
AspLEI	GCGC	2 cut(s)	221, 1628
AspS9I	GGNCC	2 cut(s)	352, 1338
AsuC2I	CCSGG	3 cut(s)	17, 131, 1844
AsuHPI	GGTGA	2 cut(s)	86, 1469
AsuII	TTCGAA	1 cut(s)	1201
AvaI	CYCGRG	2 cut(s)	1385, 2134
BaeGI	GKGCMC	2 cut(s)	491, 876
BaeI	ACNNNNGTAYC	2 cut(s)	1397, 1430
BamHI	GGATCC	1 cut(s)	1089
BanI	GGYRCC	4 cut(s)	412, 488, 1587, 1917
BanII	GRGCYC	3 cut(s)	792, 1679, 1851
BbsI	GAAGAC	2 cut(s)	27, 1142
Bbv12I	GWGCWC	2 cut(s)	92, 1851
BbvI	GCAGC	4 cut(s)	520, 1518, 2094, 2186
BccI	CCATC	5 cut(s)	478, 1139, 1317, 1447, 1899
BceAI	ACGGC	2 cut(s)	339, 1457
BcgI	CGANNNNNNTGC	2 cut(s)	133, 167
BciT130I	CCWGG	4 cut(s)	263, 1234, 1592, 2204
BciVI	GTATCC	1 cut(s)	672
BclI	TGATCA	2 cut(s)	886, 1768
BcnI	CCSGG	3 cut(s)	17, 131, 1844
BcoDI	GTCTC	6 cut(s)	32, 1379, 1382, 1950, 2124, 2233
BfaI	CTAG	3 cut(s)	711, 906, 2304
BfmI	CTRYAG	3 cut(s)	863, 1036, 1900
BfuI	GTATCC	1 cut(s)	672
BglII	AGATCT	1 cut(s)	1773
BisI	GCNGC	7 cut(s)	534, 1532, 1624, 2009, 2108, 2175, 2276
BlpI	GCTNAGC	3 cut(s)	1619, 1629, 2120
BlsI	GCNGC	7 cut(s)	535, 1533, 1625, 2010, 2109, 2176, 2277
Bme1390I	CCNGG	7 cut(s)	17, 131, 263, 1234, 1592, 1844, 2204
BmeRI	GACNNNNNGTC	1 cut(s)	1566
BmeT110I	CYCGRG	2 cut(s)	1385, 2134
BmgT120I	GGNCC	2 cut(s)	352, 1338
BmrFI	CCNGG	7 cut(s)	17, 131, 263, 1234, 1592, 1844, 2204
BmsI	GCATC	8 cut(s)	102, 520, 630, 779, 1116, 1153, 1336, 1497
BoxI	GACNNNNGTC	1 cut(s)	1938
BpiI	GAAGAC	2 cut(s)	27, 1142
BpmI	CTGGAG	2 cut(s)	1353, 2146
Bpu10I	CCTNAGC	3 cut(s)	594, 630, 1116
Bpu1102I	GCTNAGC	3 cut(s)	1619, 1629, 2120
Bpu14I	TTCGAA	1 cut(s)	1201
BpuEI	CTTGAG	3 cut(s)	326, 681, 2079
BpuMI	CCSGG	3 cut(s)	17, 131, 1844
Bsa29I	ATCGAT	1 cut(s)	1610
BsaAI	YACGTR	1 cut(s)	93
BsaI	GGTCTC	2 cut(s)	32, 2124
BsaJI	CCNNGG	5 cut(s)	15, 52, 1232, 1341, 1913
BsaWI	WCCGGW	2 cut(s)	71, 250
Bse118I	RCCGGY	1 cut(s)	2299
Bse1I	ACTGG	2 cut(s)	175, 1787
BseAI	TCCGGA	1 cut(s)	250
BseBI	CCWGG	4 cut(s)	263, 1234, 1592, 2204
BseCI	ATCGAT	1 cut(s)	1610
BseDI	CCNNGG	5 cut(s)	15, 52, 1232, 1341, 1913
BseGI	GGATG	8 cut(s)	346, 489, 1351, 1552, 1675, 1815, 1963, 2034
BseMII	CTCAG	7 cut(s)	644, 1130, 1610, 1613, 1620, 2111, 2228
BseNI	ACTGG	2 cut(s)	175, 1787
BseRI	GAGGAG	3 cut(s)	800, 1914, 2062
BseSI	GKGCMC	2 cut(s)	491, 876
BseXI	GCAGC	4 cut(s)	520, 1518, 2094, 2186
BsgI	GTGCAG	3 cut(s)	1415, 1944, 2130
Bsh1236I	CGCG	3 cut(s)	221, 1626, 2008
Bsh1285I	CGRYCG	1 cut(s)	1055
BshFI	GGCC	6 cut(s)	354, 1340, 1472, 1595, 1912, 2011
BshNI	GGYRCC	4 cut(s)	412, 488, 1587, 1917
BshVI	ATCGAT	1 cut(s)	1610
BsiEI	CGRYCG	1 cut(s)	1055
BsiHKAI	GWGCWC	2 cut(s)	92, 1851
BsiHKCI	CYCGRG	2 cut(s)	1385, 2134
BsiSI	CCGG	6 cut(s)	17, 72, 130, 251, 1844, 2300
BsmAI	GTCTC	6 cut(s)	32, 1379, 1382, 1950, 2124, 2233
BsmBI	CGTCTC	1 cut(s)	1379
BsmI	GAATGC	3 cut(s)	272, 523, 1117
BsnI	GGCC	6 cut(s)	354, 1340, 1472, 1595, 1912, 2011
Bso31I	GGTCTC	2 cut(s)	32, 2124
BsoBI	CYCGRG	2 cut(s)	1385, 2134
Bsp119I	TTCGAA	1 cut(s)	1201
Bsp1286I	GDGCHC	6 cut(s)	92, 491, 792, 876, 1679, 1851
Bsp13I	TCCGGA	1 cut(s)	250
Bsp1720I	GCTNAGC	3 cut(s)	1619, 1629, 2120
BspACI	CCGC	6 cut(s)	546, 1418, 1477, 1624, 2008, 2276
BspANI	GGCC	6 cut(s)	354, 1340, 1472, 1595, 1912, 2011
BspCNI	CTCAG	7 cut(s)	643, 1129, 1611, 1612, 1621, 2112, 2229
BspDI	ATCGAT	1 cut(s)	1610
BspEI	TCCGGA	1 cut(s)	250
BspFNI	CGCG	3 cut(s)	221, 1626, 2008
BspMAI	CTGCAG	1 cut(s)	867
BspPI	GGATC	6 cut(s)	74, 336, 998, 1084, 1097, 1262
BspQI	GCTCTTC	2 cut(s)	1495, 2220
BspT104I	TTCGAA	1 cut(s)	1201
BspT107I	GGYRCC	4 cut(s)	412, 488, 1587, 1917
BspTNI	GGTCTC	2 cut(s)	32, 2124
BsrFI	RCCGGY	1 cut(s)	2299
BsrI	ACTGG	2 cut(s)	175, 1787
BssAI	RCCGGY	1 cut(s)	2299
BssECI	CCNNGG	5 cut(s)	15, 52, 1232, 1341, 1913
BssT1I	CCWWGG	2 cut(s)	1341, 1913
Bst2UI	CCWGG	4 cut(s)	263, 1234, 1592, 2204
Bst6I	CTCTTC	3 cut(s)	1495, 1883, 2220
BstAPI	GCANNNNNTGC	3 cut(s)	871, 1958, 2116
BstBAI	YACGTR	1 cut(s)	93
BstBI	TTCGAA	1 cut(s)	1201
BstC8I	GCNNGC	4 cut(s)	911, 973, 2301, 2318
BstDEI	CTNAG	8 cut(s)	594, 630, 1116, 1599, 1619, 1629, 2120, 2237
BstEII	GGTNACC	1 cut(s)	1007
BstF5I	GGATG	8 cut(s)	346, 489, 1351, 1552, 1675, 1815, 1963, 2034
BstFNI	CGCG	3 cut(s)	221, 1626, 2008
BstHHI	GCGC	2 cut(s)	221, 1628
BstMAI	GTCTC	6 cut(s)	32, 1379, 1382, 1950, 2124, 2233
BstMCI	CGRYCG	1 cut(s)	1055
BstNI	CCWGG	4 cut(s)	263, 1234, 1592, 2204
BstNSI	RCATGY	4 cut(s)	932, 1516, 1687, 2320
BstPAI	GACNNNNGTC	1 cut(s)	1938
BstPI	GGTNACC	1 cut(s)	1007
BstSCI	CCNGG	7 cut(s)	15, 129, 261, 1232, 1590, 1842, 2202
BstSFI	CTRYAG	3 cut(s)	863, 1036, 1900
BstSLI	GKGCMC	2 cut(s)	491, 876
BstUI	CGCG	3 cut(s)	221, 1626, 2008
BstV1I	GCAGC	4 cut(s)	520, 1518, 2094, 2186
BstV2I	GAAGAC	2 cut(s)	27, 1142
BstX2I	RGATCY	5 cut(s)	66, 328, 990, 1089, 1773
BstXI	CCANNNNNNTGG	2 cut(s)	56, 1316
BstYI	RGATCY	5 cut(s)	66, 328, 990, 1089, 1773
Bsu15I	ATCGAT	1 cut(s)	1610
BsuI	GTATCC	1 cut(s)	672
BsuRI	GGCC	6 cut(s)	354, 1340, 1472, 1595, 1912, 2011
BsuTUI	ATCGAT	1 cut(s)	1610
BtsCI	GGATG	8 cut(s)	346, 489, 1351, 1552, 1675, 1815, 1963, 2034
BtsIMutI	CAGTG	3 cut(s)	425, 2079, 2147
Cac8I	GCNNGC	4 cut(s)	911, 973, 2301, 2318
CaiI	CAGNNNCTG	2 cut(s)	1528, 1958
CfoI	GCGC	2 cut(s)	221, 1628
Cfr10I	RCCGGY	1 cut(s)	2299
Cfr13I	GGNCC	2 cut(s)	352, 1338
ClaI	ATCGAT	1 cut(s)	1610
CsiI	ACCWGGT	1 cut(s)	261
Csp6I	GTAC	9 cut(s)	118, 1155, 1249, 1275, 1414, 1516, 1805, 1840, 1897
CspCI	CAANNNNNGTGG	2 cut(s)	1585, 1620
CviQI	GTAC	9 cut(s)	118, 1155, 1249, 1275, 1414, 1516, 1805, 1840, 1897
DdeI	CTNAG	8 cut(s)	594, 630, 1116, 1599, 1619, 1629, 2120, 2237
DrdI	GACNNNNNNGTC	1 cut(s)	188
DriI	GACNNNNNGTC	1 cut(s)	1566
DseDI	GACNNNNNNGTC	1 cut(s)	188
EaeI	YGGCCR	1 cut(s)	1470
Eam1104I	CTCTTC	3 cut(s)	1495, 1883, 2220
Eam1105I	GACNNNNNGTC	1 cut(s)	1566
EarI	CTCTTC	3 cut(s)	1495, 1883, 2220
Ecl136II	GAGCTC	1 cut(s)	1849
Eco130I	CCWWGG	2 cut(s)	1341, 1913
Eco24I	GRGCYC	3 cut(s)	792, 1679, 1851
Eco31I	GGTCTC	2 cut(s)	32, 2124
Eco53kI	GAGCTC	1 cut(s)	1849
Eco57I	CTGAAG	3 cut(s)	534, 687, 2272
Eco88I	CYCGRG	2 cut(s)	1385, 2134
Eco91I	GGTNACC	1 cut(s)	1007
EcoICRI	GAGCTC	1 cut(s)	1849
EcoO65I	GGTNACC	1 cut(s)	1007
EcoRI	GAATTC	1 cut(s)	1104
EcoRII	CCWGG	4 cut(s)	261, 1232, 1590, 2202
EcoT14I	CCWWGG	2 cut(s)	1341, 1913
EcoT38I	GRGCYC	3 cut(s)	792, 1679, 1851
ErhI	CCWWGG	2 cut(s)	1341, 1913
Esp3I	CGTCTC	1 cut(s)	1379
FalI	AAGNNNNNCTT	4 cut(s)	186, 218, 672, 704
FauI	CCCGC	1 cut(s)	1425
FauNDI	CATATG	2 cut(s)	984, 1467
FbaI	TGATCA	2 cut(s)	886, 1768
Fnu4HI	GCNGC	7 cut(s)	534, 1532, 1624, 2009, 2108, 2175, 2276
FokI	GGATG	8 cut(s)	353, 496, 1358, 1539, 1682, 1802, 1950, 2041
FriOI	GRGCYC	3 cut(s)	792, 1679, 1851
Fsp4HI	GCNGC	7 cut(s)	534, 1532, 1624, 2009, 2108, 2175, 2276
FspBI	CTAG	3 cut(s)	711, 906, 2304
GlaI	GCGC	2 cut(s)	220, 1627
GluI	GCNGC	7 cut(s)	534, 1532, 1624, 2009, 2108, 2175, 2276
GsuI	CTGGAG	2 cut(s)	1353, 2146
HaeIII	GGCC	6 cut(s)	354, 1340, 1472, 1595, 1912, 2011
HapII	CCGG	6 cut(s)	17, 72, 130, 251, 1844, 2300
HhaI	GCGC	2 cut(s)	221, 1628
Hin6I	GCGC	2 cut(s)	219, 1626
HinP1I	GCGC	2 cut(s)	219, 1626
HincII	GTYRAC	1 cut(s)	901
HindII	GTYRAC	1 cut(s)	901
HindIII	AAGCTT	5 cut(s)	200, 504, 596, 699, 2261
HinfI	GANTC	7 cut(s)	223, 979, 1025, 1369, 1955, 2064, 2250
HpaI	GTTAAC	1 cut(s)	901
HpaII	CCGG	6 cut(s)	17, 72, 130, 251, 1844, 2300
HphI	GGTGA	2 cut(s)	86, 1469
Hpy166II	GTNNAC	4 cut(s)	901, 1251, 1402, 1482
Hpy188I	TCNGA	9 cut(s)	372, 529, 572, 775, 1110, 1602, 1773, 2017, 2169
Hpy8I	GTNNAC	4 cut(s)	901, 1251, 1402, 1482
Hpy99I	CGWCG	1 cut(s)	301
HpyCH4IV	ACGT	2 cut(s)	92, 388
HpyF3I	CTNAG	8 cut(s)	594, 630, 1116, 1599, 1619, 1629, 2120, 2237
HpySE526I	ACGT	2 cut(s)	92, 388
HspAI	GCGC	2 cut(s)	219, 1626
Kpn2I	TCCGGA	1 cut(s)	250
KroI	GCCGGC	1 cut(s)	2299
KroNI	GCCGGC	1 cut(s)	2301
Ksp22I	TGATCA	2 cut(s)	886, 1768
KspAI	GTTAAC	1 cut(s)	901
LguI	GCTCTTC	2 cut(s)	1495, 2220
LmnI	GCTCC	5 cut(s)	548, 729, 787, 1682, 1846
Lsp1109I	GCAGC	4 cut(s)	520, 1518, 2094, 2186
LweI	GCATC	8 cut(s)	102, 520, 630, 779, 1116, 1153, 1336, 1497
MabI	ACCWGGT	1 cut(s)	261
MaeI	CTAG	3 cut(s)	711, 906, 2304
MaeII	ACGT	2 cut(s)	92, 388
MaeIII	GTNAC	5 cut(s)	74, 166, 179, 1007, 2191
MfeI	CAATTG	1 cut(s)	1287
MflI	RGATCY	5 cut(s)	66, 328, 990, 1089, 1773
MhlI	GDGCHC	6 cut(s)	92, 491, 792, 876, 1679, 1851
MlyI	GAGTC	1 cut(s)	217
MmeI	TCCRAC	1 cut(s)	1735
MroI	TCCGGA	1 cut(s)	250
MroNI	GCCGGC	1 cut(s)	2299
MroXI	GAANNNNTTC	2 cut(s)	507, 2285
MseI	TTAA	6 cut(s)	138, 198, 615, 758, 900, 1500
MslI	CAYNNNNRTG	6 cut(s)	54, 418, 528, 1314, 1437, 1968
MspA1I	CMGCKG	1 cut(s)	2278
MspI	CCGG	6 cut(s)	17, 72, 130, 251, 1844, 2300
MspR9I	CCNGG	7 cut(s)	17, 131, 263, 1234, 1592, 1844, 2204
MunI	CAATTG	1 cut(s)	1287
Mva1269I	GAATGC	3 cut(s)	272, 523, 1117
MvaI	CCWGG	4 cut(s)	263, 1234, 1592, 2204
MvnI	CGCG	3 cut(s)	221, 1626, 2008
NaeI	GCCGGC	1 cut(s)	2301
NciI	CCSGG	3 cut(s)	17, 131, 1844
NdeI	CATATG	2 cut(s)	984, 1467
NgoMIV	GCCGGC	1 cut(s)	2299
NmeAIII	GCCGAG	1 cut(s)	669
NmuCI	GTSAC	2 cut(s)	74, 179
NspI	RCATGY	4 cut(s)	932, 1516, 1687, 2320
NspV	TTCGAA	1 cut(s)	1201
OliI	CACNNNNGTG	2 cut(s)	54, 418
PaeI	GCATGC	1 cut(s)	2320
PaeR7I	CTCGAG	1 cut(s)	1385
PciI	ACATGT	2 cut(s)	1512, 1683
PciSI	GCTCTTC	2 cut(s)	1495, 2220
PcsI	WCGNNNNNNNCGW	1 cut(s)	1163
PctI	GAATGC	3 cut(s)	272, 523, 1117
PdiI	GCCGGC	1 cut(s)	2301
PdmI	GAANNNNTTC	2 cut(s)	507, 2285
PfeI	GAWTC	6 cut(s)	979, 1025, 1369, 1955, 2064, 2250
PflMI	CCANNNNNTGG	2 cut(s)	2074, 2209
PkrI	GCNGC	7 cut(s)	535, 1533, 1625, 2010, 2109, 2176, 2277
PleI	GAGTC	1 cut(s)	217
PpsI	GAGTC	1 cut(s)	217
Ppu21I	YACGTR	1 cut(s)	93
PscI	ACATGT	2 cut(s)	1512, 1683
PshAI	GACNNNNGTC	1 cut(s)	1938
Psp124BI	GAGCTC	1 cut(s)	1851
Psp6I	CCWGG	4 cut(s)	261, 1232, 1590, 2202
PspEI	GGTNACC	1 cut(s)	1007
PspGI	CCWGG	4 cut(s)	261, 1232, 1590, 2202
PspPI	GGNCC	2 cut(s)	352, 1338
PstI	CTGCAG	1 cut(s)	867
PstNI	CAGNNNCTG	2 cut(s)	1528, 1958
PsuI	RGATCY	5 cut(s)	66, 328, 990, 1089, 1773
RsaI	GTAC	9 cut(s)	119, 1156, 1250, 1276, 1415, 1517, 1806, 1841, 1898
RsaNI	GTAC	9 cut(s)	118, 1155, 1249, 1275, 1414, 1516, 1805, 1840, 1897
RseI	CAYNNNNRTG	6 cut(s)	54, 418, 528, 1314, 1437, 1968
SacI	GAGCTC	1 cut(s)	1851
SapI	GCTCTTC	2 cut(s)	1495, 2220
SaqAI	TTAA	6 cut(s)	138, 198, 615, 758, 900, 1500
SatI	GCNGC	7 cut(s)	534, 1532, 1624, 2009, 2108, 2175, 2276
Sau96I	GGNCC	2 cut(s)	352, 1338
SchI	GAGTC	1 cut(s)	217
ScrFI	CCNGG	7 cut(s)	17, 131, 263, 1234, 1592, 1844, 2204
SduI	GDGCHC	6 cut(s)	92, 491, 792, 876, 1679, 1851
SexAI	ACCWGGT	1 cut(s)	261
SfaNI	GCATC	8 cut(s)	102, 520, 630, 779, 1116, 1153, 1336, 1497
SfcI	CTRYAG	3 cut(s)	863, 1036, 1900
Sfr274I	CTCGAG	1 cut(s)	1385
SfuI	TTCGAA	1 cut(s)	1201
SlaI	CTCGAG	1 cut(s)	1385
SmiMI	CAYNNNNRTG	6 cut(s)	54, 418, 528, 1314, 1437, 1968
SmlI	CTYRAG	4 cut(s)	305, 696, 1385, 2094
SmoI	CTYRAG	4 cut(s)	305, 696, 1385, 2094
SphI	GCATGC	1 cut(s)	2320
SsiI	CCGC	6 cut(s)	546, 1418, 1477, 1624, 2008, 2276
SspMI	CTAG	3 cut(s)	711, 906, 2304
SstI	GAGCTC	1 cut(s)	1851
StyD4I	CCNGG	7 cut(s)	15, 129, 261, 1232, 1590, 1842, 2202
StyI	CCWWGG	2 cut(s)	1341, 1913
TaiI	ACGT	2 cut(s)	95, 391
TaqI	TCGA	8 cut(s)	192, 226, 278, 1051, 1201, 1386, 1610, 1656
TaqII	GACCGA	1 cut(s)	1069
TatI	WGTACW	3 cut(s)	1248, 1274, 1515
TauI	GCSGC	3 cut(s)	1626, 2011, 2278
TfiI	GAWTC	6 cut(s)	979, 1025, 1369, 1955, 2064, 2250
Tru1I	TTAA	6 cut(s)	138, 198, 615, 758, 900, 1500
Tru9I	TTAA	6 cut(s)	138, 198, 615, 758, 900, 1500
TscAI	CASTG	3 cut(s)	425, 2079, 2154
TseFI	GTSAC	2 cut(s)	74, 179
TseI	GCWGC	4 cut(s)	533, 1531, 2107, 2174
Tsp45I	GTSAC	2 cut(s)	74, 179
TspGWI	ACGGA	2 cut(s)	2187, 2243
TspRI	CASTG	3 cut(s)	425, 2079, 2154
Van91I	CCANNNNNTGG	2 cut(s)	2074, 2209
XapI	RAATTY	4 cut(s)	1104, 1461, 1985, 2281
XceI	RCATGY	4 cut(s)	932, 1516, 1687, 2320
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2075
XhoI	CTCGAG	1 cut(s)	1385
XmnI	GAANNNNTTC	2 cut(s)	507, 2285
XspI	CTAG	3 cut(s)	711, 906, 2304

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.