Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011643

Physical Location & Seq

Genomic Coordinates Reverse (-)

7284809 .. 7287417

Gene Structure 2609 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1062100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2388 bp

ATGAAGTCACAAGCAGCACCTAACCAAGAGGACTACAAGTTGAAGGACACGAAGCCCCAGCTCGGGGAACGATGGCCACATGGAGGACTTCGCGGTGGAGGAGGGTGGATAAGCAGCGATAGAGCTACGAGCACTTACGATCTCGTTGAGCAGATGTTTTATCTATATGTAAGAGTTGAGAAAGCCAAAGATCTTCCGACAAACCCCGTGACGGGAAGCTGTGATCCATATGTACAAGTGAAGCTGGGAAATTACAAGGGCAAAACAAAGCACTTTGAGAAGAAGACAAACCCTGAATGGAAGGAAGTTTTTGCATTTGCAAAGGAGAAGATTCAGTCTTCGGTACTCGAAGTTTATGTCAAGGATAAAGGGATGGTGGCAAGAGATGACTATGTTGGGAAGGTGGTGTTTGACATGCATGAGGTGCCGACTAGAGTTCCACCAGATAGCCCTTTGGCACCTCAATGGTACAGATTAGAAGACCGGCGGAGAGATACTAAGGTTAGAGGAGAGGTGATGCTTGCAGTTTGGATGGGTACACAGGCTGATGAAGCTTTCCCAGAAGCCTGGCACTCAGACGCTGCTTCAGTTCACGGAGAGGGAGTTTTCAGCATTCGCTCCAAGGTTTATGTCTCGCCGAAGCTGTGGTACCTTAGAGTTAATGTAATCGAAGCTCAGGATGTGGAGCCACACGATAGAAGCCAACCACCACAAGCTTTCGTCAAGGCTCATGTCGGAAATCAAACACTTAAAACAAAGGTATCTCCAACCAGGACAGCGAATCCCATGTGGAACGAAGACTTGGTCTTCGTAGCAGCTGAGCCTTTTGAAGAACAGCTTGTGCTTACAGTTGAAAATAAAGTGAGTGCTGCAAAAGATGAACATGTGGGAAAAATAAACCTGCCGCTGACAATCTTTGAGAGGCGGTTGGATCACAGGCCAGTCCATTCACGTTGGTTCAACCTCGAAAAATTTGGTTTTGGTGCTTTGGAGGGAGACAAGAGGCACGACCACAAGTTTTCGACCAGAGTCCATCTCAGAGTCTGTCTTGAGGGTGCTTACCATGTACTAGATGAATCAACCTTGTACATAAGCGACGTGAGGCCTACTGCCAGGCAGCTATGGAAGCATCCAATTGGGATTCTTGAAGTAGGGATCTTAAGCGCGCAAGGACTTCTTCCAATGAAGAACAAGGATGACAAAGCAACAACAGATGCCTACTGCGTAGCCAAGTACGGGCAGAAATGGGTGAGGACCAGAACAATCATCGAAAGCTTCAGTCCCAAGTGGAATGAACAATATACATGGGAGGTCTACGACCCTTGCACGGTGATCACACTGGGAGTTTTCGACAACTGCCACCTCGGTGGAAATGAGAAACCAATTTCAGGCAGTGGAGCTAAAAATGACTCGAGAATTGGCAAGGTGAGAATTCGGCTATCAACCCTAGAAATGGATCGGATTTATACAAACTCTTATCCGCTTCTAGTCCTACAACCATCCGGATTGAAAAAAATGGGAGAGCTCCAATTAGCAGTAAGATTTACATGTCTTTCACTAACTAATATAATTTACCTCTACGGCCACCCCTTGCTGCCAAAAATGCATTATCTGCATCCATTCACTGTAAATCAGTTGGATAGTTTGAGATATCAGGCAATGAACATTGTAGCCGTGAGACTTGGCAGAGCTGAACCACCACTAAGGAAAGAGGTTGTTGAGTACATGCTAGATGTAGACTCCCACGTGTGGAGCATGAGAAGAAGTAAGGCTAACTTTTTTAGAATTGTCTCACTATTCTCCGGCCTCATCTCCATGAGCAGGTGGCTTGGTGAAGTTCGTCATTGGAAAAACCCGATCACTACTGTTTTAGTCCATTTCCTGTTTTTCCTACTGATCTGCTACCCTGAATTGATTCTGCCAACTATCTTTCTCTACATGTTTCTCATCGGATTATGGAACTTCCGCTTCCGTCCAAGGCTTCCGCCTCACATGGACACTAAACTTTCATGGGCAGAGGCAGTTCACCCGGATGAATTGGATGAAGAGTTTGACACCTTCCCTACGTCTAAGGGTCAAGATGTCGTGAGGATGAGGTACGACAGACTAAGAAGTGTGGCTGGAAGAATTCAGACAGTGGTTGGAGACATTGCAACTCAGGGAGAGAGATTTCAGGCGGTCCTTAGCTGGAGAGACCCAAGAGCAAGCAGCCTGTTTGTATTTTTGTGTCTAATTGCCGCTGTGGTGCTCTACGTGACACCATTCAAGATGATTGCTCTGGCTGCAGGCATTGTTTGGCTAAGGCATCCTAGATTCCGAAGCAAGCTACCTTCGATTCCAAGCAATTTCTTCAGAAGATTGCCATCTCGTGCAGATAGCATGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005215 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008565 GO:0009314 GO:0009416 GO:0009506 GO:0009510 GO:0009511 GO:0009628 GO:0009648 GO:0009791 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0022414 GO:0023052 GO:0030054 GO:0031224 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033036 GO:0034613 GO:0035556 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0045184 GO:0046872 GO:0048518 GO:0048571 GO:0048573 GO:0048574 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 PRT_C

Protein Analysis

796

Amino Acids

91.41

Weight (kDa)

9.29

Isoelectric Point (pI)

44.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	54 - 159	5e-21	C2 domain
C2	PF00168	216 - 322	2.8e-19	C2 domain
C2	PF00168	380 - 495	1.8e-19	C2 domain
PRT_C	PF08372	640 - 795	5.7e-80	Plant phosphoribosyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06850
fragaria_vesca	FvH4_6g22780 FvH4_6g22780
malus_domestica	MD12G1062100.v1.1 MD14G1056400.v1.1
prunus_persica	Prupe.7G072800_v2.0.a1
pyrus_communis	pycom12g05490 pycom14g04620
rosa_chinensis	RchiOBHm_Chr3g0480381
rosa_laevigata	RLG00000023487
rosa_multiflora	Rmu_sc0002205.1_g000002 Rmu_sc0003742.1_g000015
rosa_roxburghii	Rroxscaffold_6G00401430
rosa_rugosa	Rorug03G0183500
rosa_samantha	Rh3AG235000 Rh3BG267500 Rh3CG264800 Rh3DG260700
rosa_wichuraiana	Rw3G021090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1812
Acc36I	ACCTGC	2 cut(s)	909, 1812
Acc65I	GGTACC	1 cut(s)	648
AccB1I	GGYRCC	3 cut(s)	424, 457, 648
AccI	GTMKAC	2 cut(s)	1314, 1737
AccII	CGCG	2 cut(s)	93, 1166
AccIII	TCCGGA	1 cut(s)	1502
AciI	CCGC	9 cut(s)	93, 487, 905, 925, 1481, 1966, 1985, 2177, 2238
AclWI	GGATC	4 cut(s)	218, 939, 1163, 1464
AcoI	YGGCCR	2 cut(s)	74, 1582
AcsI	RAATTY	3 cut(s)	971, 1431, 2127
AcuI	CTGAAG	3 cut(s)	570, 1261, 2335
AcvI	CACGTG	1 cut(s)	1747
AflII	CTTAAG	1 cut(s)	1159
AflIII	ACRYGT	4 cut(s)	883, 1547, 1746, 1938
AgsI	TTSAA	7 cut(s)	43, 830, 854, 961, 1148, 1510, 2266
AjiI	CACGTC	1 cut(s)	1099
AjnI	CCWGG	3 cut(s)	566, 770, 1112
AjuI	GAANNNNNNNTTGG	2 cut(s)	785, 817
AleI	CACNNNNGTG	1 cut(s)	1365
Alw21I	GWGCWC	3 cut(s)	134, 1527, 2250
Alw26I	GTCTC	6 cut(s)	637, 990, 1672, 1795, 2139, 2187
AlwI	GGATC	4 cut(s)	218, 939, 1163, 1464
AlwNI	CAGNNNCTG	2 cut(s)	581, 1044
Ama87I	CYCGRG	2 cut(s)	62, 1411
Aor13HI	TCCGGA	1 cut(s)	1502
AoxI	GGCC	5 cut(s)	74, 938, 1103, 1582, 1804
ApeKI	GCWGC	9 cut(s)	14, 114, 581, 815, 869, 1117, 1594, 2208, 2282
ApoI	RAATTY	3 cut(s)	971, 1431, 2127
ArsI	GACNNNNNNTTYG	6 cut(s)	342, 374, 789, 791, 821, 823
Asp700I	GAANNNNTTC	1 cut(s)	1914
Asp718I	GGTACC	1 cut(s)	648
AspLEI	GCGC	2 cut(s)	1166, 1168
AspS9I	GGNCC	2 cut(s)	1254, 2179
AsuC2I	CCSGG	1 cut(s)	2030
AsuHPI	GGTGA	6 cut(s)	526, 1261, 1342, 1438, 1844, 2018
AvaI	CYCGRG	2 cut(s)	62, 1411
AvaII	GGWCC	2 cut(s)	1254, 2179
BaeI	ACNNNNGTAYC	4 cut(s)	486, 519, 744, 777
BalI	TGGCCA	1 cut(s)	76
BanI	GGYRCC	3 cut(s)	424, 457, 648
BanII	GRGCYC	1 cut(s)	1527
BarI	GAAGNNNNNNTAC	2 cut(s)	632, 664
BauI	CACGAG	1 cut(s)	2367
BbrPI	CACGTG	1 cut(s)	1747
BbsI	GAAGAC	5 cut(s)	290, 330, 486, 799, 804
Bbv12I	GWGCWC	3 cut(s)	134, 1527, 2250
BbvI	GCAGC	9 cut(s)	26, 126, 568, 827, 856, 1129, 1581, 2220, 2269
BccI	CCATC	6 cut(s)	66, 367, 526, 1041, 1507, 2371
BceAI	ACGGC	2 cut(s)	1597, 1658
BciT130I	CCWGG	3 cut(s)	568, 772, 1114
BclI	TGATCA	1 cut(s)	1332
BcnI	CCSGG	1 cut(s)	2030
BcoDI	GTCTC	6 cut(s)	637, 990, 1672, 1795, 2139, 2187
BfaI	CTAG	6 cut(s)	432, 1070, 1448, 1487, 1730, 2310
BfmI	CTRYAG	1 cut(s)	2283
BfrI	CTTAAG	1 cut(s)	1159
BfuAI	ACCTGC	2 cut(s)	909, 1812
BglII	AGATCT	1 cut(s)	190
BlpI	GCTNAGC	1 cut(s)	819
Bme1390I	CCNGG	4 cut(s)	568, 772, 1114, 2030
Bme18I	GGWCC	2 cut(s)	1254, 2179
BmeT110I	CYCGRG	2 cut(s)	62, 1411
BmgBI	CACGTC	1 cut(s)	1099
BmgT120I	GGNCC	2 cut(s)	1254, 2179
BmiI	GGNNCC	4 cut(s)	426, 459, 650, 687
BmrFI	CCNGG	4 cut(s)	568, 772, 1114, 2030
BmrI	ACTGGG	1 cut(s)	1349
BmsI	GCATC	5 cut(s)	507, 1138, 1204, 1624, 2314
BmuI	ACTGGG	1 cut(s)	1349
BoxI	GACNNNNGTC	1 cut(s)	1028
BpiI	GAAGAC	5 cut(s)	290, 330, 486, 799, 804
BplI	GAGNNNNNCTC	2 cut(s)	1020, 1052
BpmI	CTGGAG	1 cut(s)	2209
Bpu10I	CCTNAGC	3 cut(s)	675, 2183, 2300
Bpu1102I	GCTNAGC	1 cut(s)	819
BpuEI	CTTGAG	1 cut(s)	1070
BpuMI	CCSGG	1 cut(s)	2030
BsaAI	YACGTR	2 cut(s)	1747, 2254
BsaBI	GATNNNNATC	1 cut(s)	2362
BsaI	GGTCTC	1 cut(s)	2187
BsaJI	CCNNGG	3 cut(s)	621, 1363, 1976
BsaWI	WCCGGW	1 cut(s)	1502
BsaXI	ACNNNNNCTCC	2 cut(s)	588, 618
Bse118I	RCCGGY	1 cut(s)	483
Bse1I	ACTGG	2 cut(s)	941, 1344
Bse3DI	GCAATG	2 cut(s)	1665, 2148
Bse8I	GATNNNNATC	1 cut(s)	2362
BseAI	TCCGGA	1 cut(s)	1502
BseBI	CCWGG	3 cut(s)	568, 772, 1114
BseDI	CCNNGG	3 cut(s)	621, 1363, 1976
BseJI	GATNNNNATC	1 cut(s)	2362
BseMI	GCAATG	2 cut(s)	1665, 2148
BseMII	CTCAG	5 cut(s)	588, 689, 810, 1051, 2171
BseNI	ACTGG	2 cut(s)	941, 1344
BsePI	GCGCGC	1 cut(s)	1164
BseRI	GAGGAG	2 cut(s)	114, 522
BseXI	GCAGC	9 cut(s)	26, 126, 568, 827, 856, 1129, 1581, 2220, 2269
BseYI	CCCAGC	2 cut(s)	57, 244
Bsh1236I	CGCG	2 cut(s)	93, 1166
BshFI	GGCC	5 cut(s)	76, 940, 1105, 1584, 1806
BshNI	GGYRCC	3 cut(s)	424, 457, 648
BsiHKAI	GWGCWC	3 cut(s)	134, 1527, 2250
BsiHKCI	CYCGRG	2 cut(s)	62, 1411
BsiSI	CCGG	4 cut(s)	484, 1503, 1803, 2030
BslFI	GGGAC	1 cut(s)	1266
BsmAI	GTCTC	6 cut(s)	637, 990, 1672, 1795, 2139, 2187
BsmFI	GGGAC	1 cut(s)	1266
BsmI	GAATGC	1 cut(s)	612
BsnI	GGCC	5 cut(s)	76, 940, 1105, 1584, 1806
Bso31I	GGTCTC	1 cut(s)	2187
BsoBI	CYCGRG	2 cut(s)	62, 1411
Bsp1286I	GDGCHC	3 cut(s)	134, 1527, 2250
Bsp13I	TCCGGA	1 cut(s)	1502
Bsp1407I	TGTACA	2 cut(s)	232, 1086
Bsp143I	GATC	9 cut(s)	139, 190, 223, 931, 1155, 1332, 1456, 1857, 1896
Bsp1720I	GCTNAGC	1 cut(s)	819
BspACI	CCGC	9 cut(s)	93, 487, 905, 925, 1481, 1966, 1985, 2177, 2238
BspANI	GGCC	5 cut(s)	76, 940, 1105, 1584, 1806
BspCNI	CTCAG	5 cut(s)	587, 688, 811, 1050, 2170
BspEI	TCCGGA	1 cut(s)	1502
BspFNI	CGCG	2 cut(s)	93, 1166
BspLI	GGNNCC	4 cut(s)	426, 459, 650, 687
BspMAI	CTGCAG	1 cut(s)	2287
BspMI	ACCTGC	2 cut(s)	909, 1812
BspPI	GGATC	4 cut(s)	218, 939, 1163, 1464
BspT107I	GGYRCC	3 cut(s)	424, 457, 648
BspTI	CTTAAG	1 cut(s)	1159
BspTNI	GGTCTC	1 cut(s)	2187
BsrDI	GCAATG	2 cut(s)	1665, 2148
BsrFI	RCCGGY	1 cut(s)	483
BsrGI	TGTACA	2 cut(s)	232, 1086
BsrI	ACTGG	2 cut(s)	941, 1344
BssAI	RCCGGY	1 cut(s)	483
BssECI	CCNNGG	3 cut(s)	621, 1363, 1976
BssHII	GCGCGC	1 cut(s)	1164
BssMI	GATC	9 cut(s)	139, 190, 223, 931, 1155, 1332, 1456, 1857, 1896
BssSI	CACGAG	1 cut(s)	2367
BssT1I	CCWWGG	2 cut(s)	621, 1976
Bst2BI	CACGAG	1 cut(s)	2367
Bst2UI	CCWGG	3 cut(s)	568, 772, 1114
Bst4CI	ACNGT	5 cut(s)	850, 1330, 1627, 1867, 2137
Bst6I	CTCTTC	1 cut(s)	2040
BstAFI	CTTAAG	1 cut(s)	1159
BstAPI	GCANNNNNTGC	2 cut(s)	424, 1612
BstAUI	TGTACA	2 cut(s)	232, 1086
BstBAI	YACGTR	2 cut(s)	1747, 2254
BstC8I	GCNNGC	6 cut(s)	522, 1166, 2206, 2287, 2324, 2381
BstFNI	CGCG	2 cut(s)	93, 1166
BstHHI	GCGC	2 cut(s)	1166, 1168
BstKTI	GATC	9 cut(s)	142, 193, 226, 934, 1158, 1335, 1459, 1860, 1899
BstMAI	GTCTC	6 cut(s)	637, 990, 1672, 1795, 2139, 2187
BstMBI	GATC	9 cut(s)	139, 190, 223, 931, 1155, 1332, 1456, 1857, 1896
BstMWI	GCNNNNNNNGC	6 cut(s)	424, 551, 1126, 1603, 1612, 2282
BstNI	CCWGG	3 cut(s)	568, 772, 1114
BstNSI	RCATGY	6 cut(s)	418, 887, 1551, 1729, 1942, 2383
BstPAI	GACNNNNGTC	1 cut(s)	1028
BstSCI	CCNGG	4 cut(s)	566, 770, 1112, 2028
BstSFI	CTRYAG	1 cut(s)	2283
BstUI	CGCG	2 cut(s)	93, 1166
BstV1I	GCAGC	9 cut(s)	26, 126, 568, 827, 856, 1129, 1581, 2220, 2269
BstV2I	GAAGAC	5 cut(s)	290, 330, 486, 799, 804
BstX2I	RGATCY	2 cut(s)	190, 1155
BstXI	CCANNNNNNTGG	2 cut(s)	567, 1367
BstYI	RGATCY	2 cut(s)	190, 1155
BsuRI	GGCC	5 cut(s)	76, 940, 1105, 1584, 1806
BtrI	CACGTC	1 cut(s)	1099
BtsI	GCAGTG	1 cut(s)	1399
BtsIMutI	CAGTG	4 cut(s)	1337, 1399, 1623, 2142
BveI	ACCTGC	2 cut(s)	909, 1812
Cac8I	GCNNGC	6 cut(s)	522, 1166, 2206, 2287, 2324, 2381
CaiI	CAGNNNCTG	2 cut(s)	581, 1044
CfoI	GCGC	2 cut(s)	1166, 1168
Cfr10I	RCCGGY	1 cut(s)	483
Cfr13I	GGNCC	2 cut(s)	1254, 2179
CseI	GACGC	1 cut(s)	587
DpnI	GATC	9 cut(s)	141, 192, 225, 933, 1157, 1334, 1458, 1859, 1898
DpnII	GATC	9 cut(s)	139, 190, 223, 931, 1155, 1332, 1456, 1857, 1896
EaeI	YGGCCR	2 cut(s)	74, 1582
Eam1104I	CTCTTC	1 cut(s)	2040
EarI	CTCTTC	1 cut(s)	2040
EciI	GGCGGA	2 cut(s)	502, 1974
Ecl136II	GAGCTC	1 cut(s)	1525
Eco130I	CCWWGG	2 cut(s)	621, 1976
Eco147I	AGGCCT	1 cut(s)	1105
Eco24I	GRGCYC	1 cut(s)	1527
Eco31I	GGTCTC	1 cut(s)	2187
Eco32I	GATATC	1 cut(s)	1652
Eco47I	GGWCC	2 cut(s)	1254, 2179
Eco53kI	GAGCTC	1 cut(s)	1525
Eco57I	CTGAAG	3 cut(s)	570, 1261, 2335
Eco72I	CACGTG	1 cut(s)	1747
Eco88I	CYCGRG	2 cut(s)	62, 1411
EcoICRI	GAGCTC	1 cut(s)	1525
EcoRI	GAATTC	2 cut(s)	1431, 2127
EcoRII	CCWGG	3 cut(s)	566, 770, 1112
EcoRV	GATATC	1 cut(s)	1652
EcoT14I	CCWWGG	2 cut(s)	621, 1976
EcoT22I	ATGCAT	2 cut(s)	420, 1608
EcoT38I	GRGCYC	1 cut(s)	1527
ErhI	CCWWGG	2 cut(s)	621, 1976
FalI	AAGNNNNNCTT	6 cut(s)	822, 854, 1161, 1193, 1760, 1792
FaqI	GGGAC	1 cut(s)	1266
FauNDI	CATATG	1 cut(s)	229
FbaI	TGATCA	1 cut(s)	1332
FblI	GTMKAC	2 cut(s)	1314, 1737
FriOI	GRGCYC	1 cut(s)	1527
FspBI	CTAG	6 cut(s)	432, 1070, 1448, 1487, 1730, 2310
GlaI	GCGC	2 cut(s)	1165, 1167
GsaI	CCCAGC	2 cut(s)	61, 248
GsuI	CTGGAG	1 cut(s)	2209
HaeIII	GGCC	5 cut(s)	76, 940, 1105, 1584, 1806
HapII	CCGG	4 cut(s)	484, 1503, 1803, 2030
HgaI	GACGC	1 cut(s)	587
HhaI	GCGC	2 cut(s)	1166, 1168
Hin6I	GCGC	2 cut(s)	1164, 1166
HinP1I	GCGC	2 cut(s)	1164, 1166
HindIII	AAGCTT	3 cut(s)	552, 714, 1273
HpaII	CCGG	4 cut(s)	484, 1503, 1803, 2030
HphI	GGTGA	6 cut(s)	526, 1261, 1342, 1438, 1844, 2018
Hpy166II	GTNNAC	5 cut(s)	539, 592, 1315, 1738, 2026
Hpy188III	TCNNGA	8 cut(s)	677, 1049, 1145, 1413, 1503, 2078, 2086, 2266
Hpy8I	GTNNAC	5 cut(s)	539, 592, 1315, 1738, 2026
Hpy99I	CGWCG	1 cut(s)	1100
HpyAV	CCTTC	5 cut(s)	37, 295, 394, 2068, 2340
HpyCH4III	ACNGT	5 cut(s)	850, 1330, 1627, 1867, 2137
HpyCH4IV	ACGT	5 cut(s)	952, 1098, 1746, 2066, 2253
HpyF10VI	GCNNNNNNNGC	6 cut(s)	424, 551, 1126, 1603, 1612, 2282
HpySE526I	ACGT	5 cut(s)	952, 1098, 1746, 2066, 2253
HspAI	GCGC	2 cut(s)	1164, 1166
Kpn2I	TCCGGA	1 cut(s)	1502
KpnI	GGTACC	1 cut(s)	652
Ksp22I	TGATCA	1 cut(s)	1332
Kzo9I	GATC	9 cut(s)	139, 190, 223, 931, 1155, 1332, 1456, 1857, 1896
LmnI	GCTCC	5 cut(s)	623, 685, 1397, 1530, 1752
Lsp1109I	GCAGC	9 cut(s)	26, 126, 568, 827, 856, 1129, 1581, 2220, 2269
LweI	GCATC	5 cut(s)	507, 1138, 1204, 1624, 2314
MaeI	CTAG	6 cut(s)	432, 1070, 1448, 1487, 1730, 2310
MaeII	ACGT	5 cut(s)	952, 1098, 1746, 2066, 2253
MaeIII	GTNAC	3 cut(s)	6, 208, 2254
MalI	GATC	9 cut(s)	141, 192, 225, 933, 1157, 1334, 1458, 1859, 1898
MboI	GATC	9 cut(s)	139, 190, 223, 931, 1155, 1332, 1456, 1857, 1896
MfeI	CAATTG	1 cut(s)	1134
MflI	RGATCY	2 cut(s)	190, 1155
MhlI	GDGCHC	3 cut(s)	134, 1527, 2250
MlsI	TGGCCA	1 cut(s)	76
MluNI	TGGCCA	1 cut(s)	76
MlyI	GAGTC	4 cut(s)	1038, 1050, 1403, 1733
MmeI	TCCRAC	6 cut(s)	221, 715, 791, 909, 1617, 2122
Mox20I	TGGCCA	1 cut(s)	76
Mph1103I	ATGCAT	2 cut(s)	420, 1608
MroI	TCCGGA	1 cut(s)	1502
MroXI	GAANNNNTTC	1 cut(s)	1914
MscI	TGGCCA	1 cut(s)	76
MseI	TTAA	3 cut(s)	660, 750, 1160
MslI	CAYNNNNRTG	4 cut(s)	463, 1365, 1814, 2031
Msp20I	TGGCCA	1 cut(s)	76
MspA1I	CMGCKG	3 cut(s)	818, 907, 2240
MspCI	CTTAAG	1 cut(s)	1159
MspI	CCGG	4 cut(s)	484, 1503, 1803, 2030
MspR9I	CCNGG	4 cut(s)	568, 772, 1114, 2030
MunI	CAATTG	1 cut(s)	1134
Mva1269I	GAATGC	1 cut(s)	612
MvaI	CCWGG	3 cut(s)	568, 772, 1114
MvnI	CGCG	2 cut(s)	93, 1166
MwoI	GCNNNNNNNGC	6 cut(s)	424, 551, 1126, 1603, 1612, 2282
NciI	CCSGG	1 cut(s)	2030
NdeI	CATATG	1 cut(s)	229
NdeII	GATC	9 cut(s)	139, 190, 223, 931, 1155, 1332, 1456, 1857, 1896
NlaIV	GGNNCC	4 cut(s)	426, 459, 650, 687
NmuCI	GTSAC	3 cut(s)	6, 208, 2254
NsiI	ATGCAT	2 cut(s)	420, 1608
NspI	RCATGY	6 cut(s)	418, 887, 1551, 1729, 1942, 2383
OliI	CACNNNNGTG	1 cut(s)	1365
PaeI	GCATGC	1 cut(s)	2383
PaeR7I	CTCGAG	1 cut(s)	1411
PaqCI	CACCTGC	1 cut(s)	1812
PauI	GCGCGC	1 cut(s)	1164
PceI	AGGCCT	1 cut(s)	1105
PciI	ACATGT	3 cut(s)	883, 1547, 1938
PctI	GAATGC	1 cut(s)	612
PdmI	GAANNNNTTC	1 cut(s)	1914
PfeI	GAWTC	7 cut(s)	331, 781, 1076, 1141, 1915, 2313, 2335
PflFI	GACNNNGTC	1 cut(s)	803
PleI	GAGTC	4 cut(s)	1037, 1049, 1403, 1733
PmaCI	CACGTG	1 cut(s)	1747
PmlI	CACGTG	1 cut(s)	1747
PpsI	GAGTC	4 cut(s)	1037, 1049, 1403, 1733
Ppu21I	YACGTR	2 cut(s)	1747, 2254
PscI	ACATGT	3 cut(s)	883, 1547, 1938
PshAI	GACNNNNGTC	1 cut(s)	1028
Psp124BI	GAGCTC	1 cut(s)	1527
Psp6I	CCWGG	3 cut(s)	566, 770, 1112
PspCI	CACGTG	1 cut(s)	1747
PspFI	CCCAGC	2 cut(s)	57, 244
PspGI	CCWGG	3 cut(s)	566, 770, 1112
PspN4I	GGNNCC	4 cut(s)	426, 459, 650, 687
PspPI	GGNCC	2 cut(s)	1254, 2179
PstI	CTGCAG	1 cut(s)	2287
PstNI	CAGNNNCTG	2 cut(s)	581, 1044
PsuI	RGATCY	2 cut(s)	190, 1155
PsyI	GACNNNGTC	1 cut(s)	803
PteI	GCGCGC	1 cut(s)	1164
PvuII	CAGCTG	1 cut(s)	818
RseI	CAYNNNNRTG	4 cut(s)	463, 1365, 1814, 2031
SacI	GAGCTC	1 cut(s)	1527
SaqAI	TTAA	3 cut(s)	660, 750, 1160
Sau3AI	GATC	9 cut(s)	139, 190, 223, 931, 1155, 1332, 1456, 1857, 1896
Sau96I	GGNCC	2 cut(s)	1254, 2179
SchI	GAGTC	4 cut(s)	1038, 1050, 1403, 1733
ScrFI	CCNGG	4 cut(s)	568, 772, 1114, 2030
SduI	GDGCHC	3 cut(s)	134, 1527, 2250
SfaNI	GCATC	5 cut(s)	507, 1138, 1204, 1624, 2314
SfcI	CTRYAG	1 cut(s)	2283
Sfr274I	CTCGAG	1 cut(s)	1411
SinI	GGWCC	2 cut(s)	1254, 2179
SlaI	CTCGAG	1 cut(s)	1411
SmiMI	CAYNNNNRTG	4 cut(s)	463, 1365, 1814, 2031
SmlI	CTYRAG	3 cut(s)	1049, 1159, 1411
SmoI	CTYRAG	3 cut(s)	1049, 1159, 1411
SphI	GCATGC	1 cut(s)	2383
SseBI	AGGCCT	1 cut(s)	1105
SsiI	CCGC	9 cut(s)	93, 487, 905, 925, 1481, 1966, 1985, 2177, 2238
SspMI	CTAG	6 cut(s)	432, 1070, 1448, 1487, 1730, 2310
SstI	GAGCTC	1 cut(s)	1527
StuI	AGGCCT	1 cut(s)	1105
StyD4I	CCNGG	4 cut(s)	566, 770, 1112, 2028
StyI	CCWWGG	2 cut(s)	621, 1976
TaaI	ACNGT	5 cut(s)	850, 1330, 1627, 1867, 2137
TaiI	ACGT	5 cut(s)	955, 1101, 1749, 2069, 2256
TaqI	TCGA	8 cut(s)	348, 669, 966, 1022, 1269, 1350, 1412, 2333
TatI	WGTACW	4 cut(s)	232, 1066, 1086, 1722
TauI	GCSGC	2 cut(s)	907, 2240
TfiI	GAWTC	7 cut(s)	331, 781, 1076, 1141, 1915, 2313, 2335
Tru1I	TTAA	3 cut(s)	660, 750, 1160
Tru9I	TTAA	3 cut(s)	660, 750, 1160
TscAI	CASTG	4 cut(s)	1344, 1399, 1630, 2142
TseFI	GTSAC	3 cut(s)	6, 208, 2254
TseI	GCWGC	9 cut(s)	14, 114, 581, 815, 869, 1117, 1594, 2208, 2282
Tsp45I	GTSAC	3 cut(s)	6, 208, 2254
TspGWI	ACGGA	2 cut(s)	609, 1961
TspRI	CASTG	4 cut(s)	1344, 1399, 1630, 2142
Tth111I	GACNNNGTC	1 cut(s)	803
Vha464I	CTTAAG	1 cut(s)	1159
VpaK11BI	GGWCC	2 cut(s)	1254, 2179
XapI	RAATTY	3 cut(s)	971, 1431, 2127
XceI	RCATGY	6 cut(s)	418, 887, 1551, 1729, 1942, 2383
XhoI	CTCGAG	1 cut(s)	1411
XmiI	GTMKAC	2 cut(s)	1314, 1737
XmnI	GAANNNNTTC	1 cut(s)	1914
XspI	CTAG	6 cut(s)	432, 1070, 1448, 1487, 1730, 2310
Zsp2I	ATGCAT	2 cut(s)	420, 1608

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.