MD12G1092800.v1.1

HAUS augmin-like complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012064
Physical Location & Seq
Reverse (-)
11949931 .. 11952829
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1092800.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGCCGACCCCTCTCTAATCGAAACGACGTCGAAGAGCAGCAGCGCCGGCGGTGGCGGTTCAGACGCATCTCGAATCGGCGACGTAAAGCAATGGCTCACTCAAGAGTTCTCCCAAGCCGGAAAAGAAGTGCCCGATTTCGAATATACCCCTCGTTCGGTGGCGTATTTACACAACCTTGCCACTCTCTCCCAAGCCCAAACTCAAGCTTCCAAGATCCTCGCTTCCGATTTCCGCCTCAAAGCCTCCGAATACCAAGCCCAAGCTGCGAGGATCAGAGAGATATTGGAGAGTGTGGGTTTGGCACAGGAGGGTTTGGGTTCGAACGTGATGTCTTCAGCTCAGGTTCTAGCGAATGTAGCCAATTTGTTGAATATAAGAGACACTGAGCTCAGCAGTTTTCTTGTGGCGATGGGGGATATATCGTTGAGGAAGACGGGTGTGGAGGAGAAGAGGGCTAAAGCGCAAAAAGAGTCTAATGTTCTTCTTGATTATACTCGAAAGGCGATAGCCAGGTTAACTTATTTGAAAAGAACACTTGCCCAACTAGAAGATGATGCAGGCCAATGTGAGGAGCATGTTGAGCATTGGAAGAACAACTTGGCGCTGATGCTTGGCAAGGAGAAACAATACAATCAAGAGACTGTCCGTTTCAAGGATACTCTTAATCGCGTGGGCTACACACCAGAGATTAGCCATGGGGTGCTGGTTGAATTGGCTGAGCATCGAAAGGAATTAGAGAAGAAGACAAAGCCCGTCCTTGATACTTTGAGAAGCTACCAAGACTTGCCTCCGGATAAAGCTTTGGCTGCTTTAGCAATTGAGGACAAGAAAAGGCAATATGCTGCTGCGGAGAAGTATCTCGAAGATGTATTGCATTCAGCTCTAGCCACTTCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005819 GO:0005856 GO:0015630 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

33.23

Weight (kDa)

5.97

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS1 PF25762 29 - 265 4e-73 HAUS augmin-like complex subunit 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012064)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41350 AT2G41350
fragaria_vesca FvH4_6g25170
malus_domestica MD12G1092800.v1.1 MD14G1084500.v1.1
prunus_persica Prupe.7G043700_v2.0.a1
pyrus_communis pycom12g07760 pycom14g07420
rosa_chinensis RchiOBHm_Chr2g0125061
rosa_laevigata RLG00000018846
rosa_multiflora Rmu_sc0003505.1_g000017
rosa_roxburghii Rroxscaffold_2G00118280 Rroxscaffold_2G00118730
rosa_rugosa Rorug02G0255300
rosa_samantha Rh2AG313800 Rh2BG322100 Rh2CG304400 Rh2DG340500
rosa_wichuraiana Rw2G025390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 32
AccII CGCG 1 cut(s) 672
AccIII TCCGGA 1 cut(s) 793
AciI CCGC 4 cut(s) 51, 57, 235, 851
AclWI GGATC 2 cut(s) 211, 281
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 321, 879
AcyI GRCGYC 1 cut(s) 29
AfiI CCNNNNNNNGG 3 cut(s) 157, 571, 655
AgsI TTSAA 4 cut(s) 373, 529, 655, 713
AjnI CCWGG 1 cut(s) 512
AjuI GAANNNNNNNTTGG 2 cut(s) 584, 616
AluBI AGCT 7 cut(s) 209, 266, 341, 391, 777, 803, 884
AluI AGCT 7 cut(s) 209, 266, 341, 391, 777, 803, 884
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 2 cut(s) 375, 635
AlwI GGATC 2 cut(s) 211, 281
Aor13HI TCCGGA 1 cut(s) 793
AoxI GGCC 2 cut(s) 3, 562
ApeKI GCWGC 6 cut(s) 39, 42, 266, 809, 845, 848
ArsI GACNNNNNNTTYG 2 cut(s) 317, 349
AspLEI GCGC 3 cut(s) 47, 466, 607
AsuII TTCGAA 2 cut(s) 141, 323
BaeGI GKGCMC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 393
BbsI GAAGAC 3 cut(s) 327, 440, 752
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 6 cut(s) 51, 54, 253, 796, 832, 835
BccI CCATC 1 cut(s) 406
BciT130I CCWGG 1 cut(s) 514
BciVI GTATCC 1 cut(s) 652
BcoDI GTCTC 2 cut(s) 375, 635
BfaI CTAG 3 cut(s) 350, 548, 887
BfoI RGCGCY 2 cut(s) 48, 608
BfuI GTATCC 1 cut(s) 652
BisI GCNGC 6 cut(s) 40, 43, 267, 810, 846, 849
BlpI GCTNAGC 2 cut(s) 392, 720
BlsI GCNGC 6 cut(s) 41, 44, 268, 811, 847, 850
Bme1390I CCNGG 1 cut(s) 514
BmrFI CCNGG 1 cut(s) 514
BmsI GCATC 4 cut(s) 77, 547, 600, 733
BpiI GAAGAC 3 cut(s) 327, 440, 752
Bpu10I CCTNAGC 1 cut(s) 342
Bpu1102I GCTNAGC 2 cut(s) 392, 720
Bpu14I TTCGAA 2 cut(s) 141, 323
BpuEI CTTGAG 2 cut(s) 87, 189
BsaHI GRCGYC 1 cut(s) 29
BsaJI CCNNGG 1 cut(s) 697
BsaWI WCCGGW 1 cut(s) 793
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 571, 655
Bse118I RCCGGY 1 cut(s) 47
Bse3DI GCAATG 1 cut(s) 98
BseAI TCCGGA 1 cut(s) 793
BseBI CCWGG 1 cut(s) 514
BseDI CCNNGG 1 cut(s) 697
BseLI CCNNNNNNNGG 3 cut(s) 157, 571, 655
BseMI GCAATG 1 cut(s) 98
BseMII CTCAG 4 cut(s) 356, 378, 406, 711
BseRI GAGGAG 2 cut(s) 461, 587
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 6 cut(s) 51, 54, 253, 796, 832, 835
Bsh1236I CGCG 1 cut(s) 672
BshFI GGCC 2 cut(s) 5, 564
BsiHKAI GWGCWC 1 cut(s) 393
BsiSI CCGG 3 cut(s) 48, 120, 794
BslI CCNNNNNNNGG 3 cut(s) 157, 571, 655
BsmAI GTCTC 2 cut(s) 375, 635
BsmI GAATGC 1 cut(s) 877
BsnI GGCC 2 cut(s) 5, 564
Bsp119I TTCGAA 2 cut(s) 141, 323
Bsp1286I GDGCHC 2 cut(s) 135, 393
Bsp13I TCCGGA 1 cut(s) 793
Bsp143I GATC 2 cut(s) 216, 273
Bsp1720I GCTNAGC 2 cut(s) 392, 720
Bsp19I CCATGG 1 cut(s) 697
BspACI CCGC 4 cut(s) 51, 57, 235, 851
BspANI GGCC 2 cut(s) 5, 564
BspCNI CTCAG 4 cut(s) 355, 379, 405, 712
BspEI TCCGGA 1 cut(s) 793
BspFNI CGCG 1 cut(s) 672
BspPI GGATC 2 cut(s) 211, 281
BspQI GCTCTTC 1 cut(s) 29
BspT104I TTCGAA 2 cut(s) 141, 323
BsrDI GCAATG 1 cut(s) 98
BsrFI RCCGGY 1 cut(s) 47
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 697
BssMI GATC 2 cut(s) 216, 273
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 697
Bst2UI CCWGG 1 cut(s) 514
Bst4CI ACNGT 1 cut(s) 646
Bst6I CTCTTC 2 cut(s) 29, 446
BstACI GRCGYC 1 cut(s) 29
BstBI TTCGAA 2 cut(s) 141, 323
BstC8I GCNNGC 2 cut(s) 49, 562
BstDEI CTNAG 4 cut(s) 342, 387, 392, 720
BstDSI CCRYGG 1 cut(s) 697
BstFNI CGCG 1 cut(s) 672
BstH2I RGCGCY 2 cut(s) 48, 608
BstHHI GCGC 3 cut(s) 47, 466, 607
BstKTI GATC 2 cut(s) 219, 276
BstMAI GTCTC 2 cut(s) 375, 635
BstMBI GATC 2 cut(s) 216, 273
BstMWI GCNNNNNNNGC 6 cut(s) 48, 266, 359, 583, 809, 815
BstNI CCWGG 1 cut(s) 514
BstNSI RCATGY 1 cut(s) 581
BstSCI CCNGG 1 cut(s) 512
BstSLI GKGCMC 1 cut(s) 135
BstUI CGCG 1 cut(s) 672
BstV1I GCAGC 6 cut(s) 51, 54, 253, 796, 832, 835
BstV2I GAAGAC 3 cut(s) 327, 440, 752
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BsuI GTATCC 1 cut(s) 652
BsuRI GGCC 2 cut(s) 5, 564
BtgI CCRYGG 1 cut(s) 697
BtgZI GCGATG 1 cut(s) 425
BtsIMutI CAGTG 1 cut(s) 384
Cac8I GCNNGC 2 cut(s) 49, 562
CfoI GCGC 3 cut(s) 47, 466, 607
Cfr10I RCCGGY 1 cut(s) 47
CseI GACGC 1 cut(s) 74
CviAII CATG 2 cut(s) 578, 698
DdeI CTNAG 4 cut(s) 342, 387, 392, 720
DpnI GATC 2 cut(s) 218, 275
DpnII GATC 2 cut(s) 216, 273
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 29, 446
EarI CTCTTC 2 cut(s) 29, 446
EciI GGCGGA 1 cut(s) 224
Ecl136II GAGCTC 1 cut(s) 391
Eco130I CCWWGG 1 cut(s) 697
Eco24I GRGCYC 1 cut(s) 393
Eco53kI GAGCTC 1 cut(s) 391
Eco57I CTGAAG 2 cut(s) 321, 879
EcoICRI GAGCTC 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 512
EcoT14I CCWWGG 1 cut(s) 697
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 697
FaeI CATG 2 cut(s) 581, 701
FaiI YATR 7 cut(s) 147, 377, 422, 495, 579, 699, 843
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FatI CATG 2 cut(s) 577, 697
Fnu4HI GCNGC 6 cut(s) 40, 43, 267, 810, 846, 849
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 6 cut(s) 40, 43, 267, 810, 846, 849
FspBI CTAG 3 cut(s) 350, 548, 887
GlaI GCGC 3 cut(s) 46, 465, 606
GluI GCNGC 6 cut(s) 40, 43, 267, 810, 846, 849
HaeII RGCGCY 2 cut(s) 48, 608
HaeIII GGCC 2 cut(s) 5, 564
HapII CCGG 3 cut(s) 48, 120, 794
HgaI GACGC 1 cut(s) 74
HhaI GCGC 3 cut(s) 47, 466, 607
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 2 cut(s) 581, 701
Hin6I GCGC 3 cut(s) 45, 464, 605
HinP1I GCGC 3 cut(s) 45, 464, 605
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HindIII AAGCTT 2 cut(s) 207, 801
HinfI GANTC 2 cut(s) 75, 473
HpaI GTTAAC 1 cut(s) 519
HpaII CCGG 3 cut(s) 48, 120, 794
Hpy166II GTNNAC 1 cut(s) 519
Hpy188I TCNGA 5 cut(s) 64, 229, 250, 278, 898
Hpy188III TCNNGA 6 cut(s) 72, 104, 488, 638, 794, 863
Hpy8I GTNNAC 1 cut(s) 519
Hpy99I CGWCG 3 cut(s) 31, 34, 86
HpyCH4III ACNGT 1 cut(s) 646
HpyCH4IV ACGT 3 cut(s) 29, 84, 327
HpyCH4V TGCA 2 cut(s) 560, 877
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 266, 359, 583, 809, 815
HpyF3I CTNAG 4 cut(s) 342, 387, 392, 720
HpySE526I ACGT 3 cut(s) 29, 84, 327
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 2 cut(s) 581, 701
HspAI GCGC 3 cut(s) 45, 464, 605
Kpn2I TCCGGA 1 cut(s) 793
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
KspAI GTTAAC 1 cut(s) 519
Kzo9I GATC 2 cut(s) 216, 273
LguI GCTCTTC 1 cut(s) 29
LmnI GCTCC 1 cut(s) 574
Lsp1109I GCAGC 6 cut(s) 51, 54, 253, 796, 832, 835
LweI GCATC 4 cut(s) 77, 547, 600, 733
MaeI CTAG 3 cut(s) 350, 548, 887
MaeII ACGT 3 cut(s) 29, 84, 327
MalI GATC 2 cut(s) 218, 275
MboI GATC 2 cut(s) 216, 273
MfeI CAATTG 1 cut(s) 819
MflI RGATCY 1 cut(s) 216
MhlI GDGCHC 2 cut(s) 135, 393
MluCI AATT 4 cut(s) 364, 713, 734, 819
MlyI GAGTC 1 cut(s) 482
MreI CGCCGGCG 1 cut(s) 47
MroI TCCGGA 1 cut(s) 793
MroNI GCCGGC 1 cut(s) 47
MseI TTAA 2 cut(s) 518, 666
MspI CCGG 3 cut(s) 48, 120, 794
MspR9I CCNGG 1 cut(s) 514
MunI CAATTG 1 cut(s) 819
Mva1269I GAATGC 1 cut(s) 877
MvaI CCWGG 1 cut(s) 514
MvnI CGCG 1 cut(s) 672
MwoI GCNNNNNNNGC 6 cut(s) 48, 266, 359, 583, 809, 815
NaeI GCCGGC 1 cut(s) 49
NcoI CCATGG 1 cut(s) 697
NdeII GATC 2 cut(s) 216, 273
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 2 cut(s) 581, 701
NspI RCATGY 1 cut(s) 581
NspV TTCGAA 2 cut(s) 141, 323
PciSI GCTCTTC 1 cut(s) 29
PctI GAATGC 1 cut(s) 877
PdiI GCCGGC 1 cut(s) 49
PfeI GAWTC 1 cut(s) 75
PkrI GCNGC 6 cut(s) 41, 44, 268, 811, 847, 850
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
Psp124BI GAGCTC 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 512
PspGI CCWGG 1 cut(s) 512
PsuI RGATCY 1 cut(s) 216
SacI GAGCTC 1 cut(s) 393
SapI GCTCTTC 1 cut(s) 29
SaqAI TTAA 2 cut(s) 518, 666
SatI GCNGC 6 cut(s) 40, 43, 267, 810, 846, 849
Sau3AI GATC 2 cut(s) 216, 273
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 1 cut(s) 514
SduI GDGCHC 2 cut(s) 135, 393
SfaNI GCATC 4 cut(s) 77, 547, 600, 733
SfuI TTCGAA 2 cut(s) 141, 323
SgrAI CRCCGGYG 1 cut(s) 47
SmlI CTYRAG 2 cut(s) 102, 204
SmoI CTYRAG 2 cut(s) 102, 204
Sse9I AATT 4 cut(s) 364, 713, 734, 819
SsiI CCGC 4 cut(s) 51, 57, 235, 851
SspMI CTAG 3 cut(s) 350, 548, 887
SstI GAGCTC 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 512
StyI CCWWGG 1 cut(s) 697
TaaI ACNGT 1 cut(s) 646
TaiI ACGT 3 cut(s) 32, 87, 330
TaqI TCGA 8 cut(s) 21, 32, 73, 141, 323, 499, 727, 864
TasI AATT 4 cut(s) 364, 713, 734, 819
TfiI GAWTC 1 cut(s) 75
Tru1I TTAA 2 cut(s) 518, 666
Tru9I TTAA 2 cut(s) 518, 666
TscAI CASTG 1 cut(s) 391
TseI GCWGC 6 cut(s) 39, 42, 266, 809, 845, 848
TspGWI ACGGA 1 cut(s) 638
TspRI CASTG 1 cut(s) 391
XceI RCATGY 1 cut(s) 581
XspI CTAG 3 cut(s) 350, 548, 887
ZraI GACGTC 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.