MD12G1100800.v1.1

SRSF protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008320
Physical Location & Seq
Forward (+)
15737238 .. 15740871
3634 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1100800.v1.1.491

Sequence Viewer

Length: 1656 bp
ATGGGGGATAAACAGGACGACGATCGGAGTCAGAGCAGCGACTACACGTCGGAGGATGAAGGCACCGAGGATTACCGGCGCGGTGGCTACCACGCCGTCCGAATCGGCGACACTTTCAAGAGCGGACGGTATGCCGTTCAGACCAAGCTCGGCTGGGGCCATTTCTCCACCGTCTGGCTCGCCTGGGACACCCAACACTCCAGATTTGTTGCTCTGAAAGTGCAAAAGAGTGCTGACCACTACACTGAGGCGGCCATGGATGAGATAACCATCTTGAAACAGATTGCAGAGGGAGACCCAGGTGATAAAAAATGCGTGGTGAAGCTTCTAGACCATTTTAAGCATTCGGGTCCGAATGGGCAACATGTTTGTATGGTTTTTGAGTACTTGGGTGATAATCTGTTGACGCTTATTAAGTATAGTGATTACCGGGGAATGCCACTACATAAGGTTAAGGAGATTTGTTATCATGTTTTGGTTGGATTGGATTATTTGCACAGACAGCTGTCGATTATACACACTGATTTGAAGCCAGAGAATATCTTGTTGATGTCAATGATAGACCCGCTCAAGGATCCGACAAAGTCAAGAGCACCTCTTATTCTTCCAGGTGGTAAAGACAAGGATGCATCGGAGTCAGGGTATGCAAAAGCGTTAAATGGGGATATGAGTCGGAACCAGAAGAAAAAGATTAGAAGAAAGGCGAAGCGTGCAGCTCAAGAGTGCGTGGATAGAGATGAAGCTGATGCTGATGTAGAAACATCCACTGAGGTAGAGGCATCTCCTAACACAAGATTGAATGTGCGTTCTGTTGAAGACCAGTCTACTAGTTCTGGCAATGCGAATAGATCATCAGATGCTGGTGGGCCAAATGGCTCTCGTCCAGGAAGTCATGGTAGTAAGAGAGGCAATCGCACTACAAGGCAGAAGTTGTTGGCATCAATTGACCTGAGGTGCAAATTGGTTGATTTTGGGAATGCATGTTGGACGTACAAACAGTTCACAAACGATATCCAAACAAGACAGTATAGGTGTCCAGAGGTGATCCTCGGCTCAAAATATTCAACCTCGGCAGATCTTTGGTCTTTTGCATGCATTTGTTTTGAGCTTGCAACTGGTGATGTACTCTTTGATCCCCACAGTGGTGACAACTTTGACAGAGATGAGGATCACTTAGCATTGATGATGGAGCTTCTTGGAATGATGCCACGAAAGATTGCCTTAGGTGGTCGATATTCTAGAGATTACTTTAATAGATACGGTGATTTGAGGCACATTCGTCGCTTGCGTTTTTGGCCCCTGAATAAGGTTCTGGTAGAAAAGTACGAATTTAGTGAGAAAGATGCAAGTGAATTAACTGACTTCCTCGTTCCCATCCTCGACTTTGTCCCTGAGAAACGGCCTACTGCTGCACAGTGCCTTGTTCATCCATGGATCAATGCAGGTCCTCGGCTACTGGAACCATCTATAGCTGCTGATAAAAAACAAGGAGTGGACAGTGACACGGCTGAAGAAAACCAGAGGGAAAAAGACGAGAGGGAGGCAATGGAGGTAGGGGTGGGAAATATCGCCATTAATTCCGATTCTAAACGGGTCAAAGATGTCCCATCCAGTAGTAAACCCTCCCTGGCAGCCGAAGGTAATCCATCCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000245 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016071 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016779 GO:0019219 GO:0019222 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0023052 GO:0031323 GO:0031325 GO:0032153 GO:0032386 GO:0032388 GO:0032507 GO:0032879 GO:0032880 GO:0033036 GO:0033120 GO:0033157 GO:0033365 GO:0034504 GO:0034613 GO:0034622 GO:0034641 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045185 GO:0045935 GO:0046483 GO:0046822 GO:0046824 GO:0046825 GO:0046827 GO:0048024 GO:0048026 GO:0048518 GO:0048522 GO:0050265 GO:0050684 GO:0050685 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051235 GO:0051252 GO:0051254 GO:0051457 GO:0051641 GO:0051651 GO:0051716 GO:0060255 GO:0060341 GO:0065003 GO:0065007 GO:0065008 GO:0070201 GO:0070569 GO:0070727 GO:0071704 GO:0071826 GO:0071840 GO:0072595 GO:0080090 GO:0090087 GO:0090304 GO:0090316 GO:0140096 GO:0140098 GO:1901360 GO:1901564 GO:1903311 GO:1903313 GO:1903827 GO:1903829 GO:1904951
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

552

Amino Acids

61.95

Weight (kDa)

6.49

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 44 - 184 1.8e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 46 - 185 2.1e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 316 - 479 4.1e-19 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1433
AccB1I GGYRCC 1 cut(s) 62
AccB7I CCANNNNNTGG 1 cut(s) 174
AccBSI CCGCTC 2 cut(s) 123, 568
AccI GTMKAC 1 cut(s) 824
AccII CGCG 1 cut(s) 81
AciI CCGC 4 cut(s) 81, 123, 251, 566
AclWI GGATC 6 cut(s) 569, 582, 1039, 1127, 1176, 1442
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 1 cut(s) 1328
AcuI CTGAAG 1 cut(s) 1530
AfaI GTAC 4 cut(s) 386, 992, 1125, 1325
AfiI CCNNNNNNNGG 4 cut(s) 174, 571, 1142, 1306
AflIII ACRYGT 2 cut(s) 45, 364
AgsI TTSAA 6 cut(s) 118, 277, 529, 799, 815, 1065
AhdI GACNNNNNGTC 1 cut(s) 46
AhlI ACTAGT 1 cut(s) 827
AjiI CACGTC 1 cut(s) 48
AjnI CCWGG 6 cut(s) 182, 298, 607, 883, 1626, 1649
AleI CACNNNNGTG 1 cut(s) 1143
AluBI AGCT 8 cut(s) 148, 325, 505, 716, 743, 1108, 1192, 1472
AluI AGCT 8 cut(s) 148, 325, 505, 716, 743, 1108, 1192, 1472
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 6 cut(s) 569, 582, 1039, 1127, 1176, 1442
AlwNI CAGNNNCTG 1 cut(s) 860
AoxI GGCC 5 cut(s) 157, 252, 866, 1295, 1400
ApeKI GCWGC 5 cut(s) 36, 713, 1409, 1472, 1631
ApoI RAATTY 1 cut(s) 1328
AseI ATTAAT 1 cut(s) 1575
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 5 cut(s) 157, 350, 866, 1296, 1445
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 7 cut(s) 314, 331, 404, 1054, 1130, 1157, 1274
AvaII GGWCC 2 cut(s) 350, 1445
AxyI CCTNAGG 2 cut(s) 950, 1222
BamHI GGATCC 1 cut(s) 574
BanI GGYRCC 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 822
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 5 cut(s) 48, 725, 1396, 1459, 1643
BccI CCATC 6 cut(s) 278, 1180, 1382, 1471, 1615, 1654
BceAI ACGGC 4 cut(s) 80, 119, 1415, 1521
BcgI CGANNNNNNTGC 2 cut(s) 1262, 1296
BciT130I CCWGG 6 cut(s) 184, 300, 609, 885, 1628, 1651
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 1 cut(s) 288
BcuI ACTAGT 1 cut(s) 827
BfaI CTAG 3 cut(s) 329, 828, 1239
BfmI CTRYAG 1 cut(s) 1467
BfuAI ACCTGC 1 cut(s) 1433
BglII AGATCT 1 cut(s) 1075
BisI GCNGC 6 cut(s) 37, 252, 714, 1410, 1473, 1632
BlsI GCNGC 6 cut(s) 38, 253, 715, 1411, 1474, 1633
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 7 cut(s) 184, 300, 431, 609, 885, 1628, 1651
Bme18I GGWCC 2 cut(s) 350, 1445
BmeRI GACNNNNNGTC 1 cut(s) 46
BmgBI CACGTC 1 cut(s) 48
BmgT120I GGNCC 5 cut(s) 157, 350, 866, 1296, 1445
BmiI GGNNCC 7 cut(s) 64, 158, 351, 576, 677, 1298, 1461
BmrFI CCNGG 7 cut(s) 184, 300, 431, 609, 885, 1628, 1651
BmsI GCATC 8 cut(s) 616, 638, 736, 788, 847, 947, 1194, 1333
BoxI GACNNNNGTC 1 cut(s) 505
BpiI GAAGAC 1 cut(s) 822
BpmI CTGGAG 1 cut(s) 184
BpuEI CTTGAG 2 cut(s) 554, 702
BpuMI CCSGG 1 cut(s) 431
BsaBI GATNNNNATC 2 cut(s) 269, 1167
BsaI GGTCTC 1 cut(s) 288
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 571, 1142, 1306
Bse118I RCCGGY 1 cut(s) 75
Bse1I ACTGG 4 cut(s) 820, 1120, 1461, 1611
Bse21I CCTNAGG 2 cut(s) 950, 1222
Bse3DI GCAATG 2 cut(s) 844, 1551
Bse8I GATNNNNATC 2 cut(s) 269, 1167
BseBI CCWGG 6 cut(s) 184, 300, 609, 885, 1628, 1651
BseGI GGATG 8 cut(s) 61, 265, 631, 761, 1374, 1426, 1607, 1646
BseJI GATNNNNATC 2 cut(s) 269, 1167
BseLI CCNNNNNNNGG 4 cut(s) 174, 571, 1142, 1306
BseMI GCAATG 2 cut(s) 844, 1551
BseMII CTCAG 4 cut(s) 237, 759, 941, 1383
BseNI ACTGG 4 cut(s) 820, 1120, 1461, 1611
BseXI GCAGC 5 cut(s) 48, 725, 1396, 1459, 1643
BseYI CCCAGC 1 cut(s) 153
BsgI GTGCAG 2 cut(s) 732, 1395
Bsh1236I CGCG 1 cut(s) 81
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 5 cut(s) 159, 254, 868, 1297, 1402
BshNI GGYRCC 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 595
BsiSI CCGG 2 cut(s) 76, 430
BslFI GGGAC 3 cut(s) 200, 1373, 1589
BslI CCNNNNNNNGG 4 cut(s) 174, 571, 1142, 1306
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 3 cut(s) 200, 1373, 1589
BsmI GAATGC 3 cut(s) 343, 441, 982
BsnI GGCC 5 cut(s) 159, 254, 868, 1297, 1402
Bso31I GGTCTC 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 595
Bsp143I GATC 8 cut(s) 22, 574, 848, 1044, 1075, 1132, 1168, 1434
Bsp19I CCATGG 2 cut(s) 255, 1430
BspACI CCGC 4 cut(s) 81, 123, 251, 566
BspANI GGCC 5 cut(s) 159, 254, 868, 1297, 1402
BspCNI CTCAG 4 cut(s) 238, 760, 942, 1384
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 7 cut(s) 64, 158, 351, 576, 677, 1298, 1461
BspMI ACCTGC 1 cut(s) 1433
BspPI GGATC 6 cut(s) 569, 582, 1039, 1127, 1176, 1442
BspT107I GGYRCC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 288
BsrBI CCGCTC 2 cut(s) 123, 568
BsrDI GCAATG 2 cut(s) 844, 1551
BsrFI RCCGGY 1 cut(s) 75
BsrI ACTGG 4 cut(s) 820, 1120, 1461, 1611
BssAI RCCGGY 1 cut(s) 75
BssMI GATC 8 cut(s) 22, 574, 848, 1044, 1075, 1132, 1168, 1434
BssT1I CCWWGG 2 cut(s) 255, 1430
Bst2UI CCWGG 6 cut(s) 184, 300, 609, 885, 1628, 1651
Bst4CI ACNGT 8 cut(s) 129, 172, 999, 1026, 1142, 1262, 1416, 1499
BstC8I GCNNGC 5 cut(s) 180, 711, 1093, 1110, 1286
BstDEI CTNAG 6 cut(s) 246, 768, 950, 1174, 1222, 1392
BstDSI CCRYGG 2 cut(s) 255, 1430
BstENI CCTNNNNNAGG 1 cut(s) 1304
BstF5I GGATG 8 cut(s) 61, 265, 631, 761, 1374, 1426, 1607, 1646
BstFNI CGCG 1 cut(s) 81
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 8 cut(s) 25, 577, 851, 1047, 1078, 1135, 1171, 1437
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 8 cut(s) 22, 574, 848, 1044, 1075, 1132, 1168, 1434
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 3 cut(s) 502, 710, 1294
BstNI CCWGG 6 cut(s) 184, 300, 609, 885, 1628, 1651
BstNSI RCATGY 3 cut(s) 368, 984, 1095
BstPAI GACNNNNGTC 1 cut(s) 505
BstSCI CCNGG 7 cut(s) 182, 298, 429, 607, 883, 1626, 1649
BstSFI CTRYAG 1 cut(s) 1467
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 5 cut(s) 48, 725, 1396, 1459, 1643
BstV2I GAAGAC 1 cut(s) 822
BstX2I RGATCY 2 cut(s) 574, 1075
BstYI RGATCY 2 cut(s) 574, 1075
Bsu36I CCTNAGG 2 cut(s) 950, 1222
BsuRI GGCC 5 cut(s) 159, 254, 868, 1297, 1402
BtgI CCRYGG 2 cut(s) 255, 1430
BtrI CACGTC 1 cut(s) 48
BtsCI GGATG 8 cut(s) 61, 265, 631, 761, 1374, 1426, 1607, 1646
BtsIMutI CAGTG 6 cut(s) 243, 519, 765, 1147, 1421, 1504
BveI ACCTGC 1 cut(s) 1433
Cac8I GCNNGC 5 cut(s) 180, 711, 1093, 1110, 1286
CaiI CAGNNNCTG 1 cut(s) 860
CfoI GCGC 1 cut(s) 81
Cfr10I RCCGGY 1 cut(s) 75
Cfr13I GGNCC 5 cut(s) 157, 350, 866, 1296, 1445
CseI GACGC 1 cut(s) 415
Csp6I GTAC 4 cut(s) 385, 991, 1124, 1324
CviAII CATG 7 cut(s) 256, 365, 470, 893, 981, 1092, 1431
CviQI GTAC 4 cut(s) 385, 991, 1124, 1324
DdeI CTNAG 6 cut(s) 246, 768, 950, 1174, 1222, 1392
DpnI GATC 8 cut(s) 24, 576, 850, 1046, 1077, 1134, 1170, 1436
DpnII GATC 8 cut(s) 22, 574, 848, 1044, 1075, 1132, 1168, 1434
DriI GACNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 252
Eam1105I GACNNNNNGTC 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 255, 1430
Eco31I GGTCTC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 1012
Eco47I GGWCC 2 cut(s) 350, 1445
Eco57I CTGAAG 1 cut(s) 1530
Eco81I CCTNAGG 2 cut(s) 950, 1222
EcoNI CCTNNNNNAGG 1 cut(s) 1304
EcoO109I RGGNCCY 1 cut(s) 1445
EcoRII CCWGG 6 cut(s) 182, 298, 607, 883, 1626, 1649
EcoRV GATATC 1 cut(s) 1012
EcoT14I CCWWGG 2 cut(s) 255, 1430
EcoT22I ATGCAT 3 cut(s) 631, 982, 1097
ErhI CCWWGG 2 cut(s) 255, 1430
FaeI CATG 7 cut(s) 259, 368, 473, 896, 984, 1095, 1434
FalI AAGNNNNNCTT 2 cut(s) 1205, 1237
FaqI GGGAC 3 cut(s) 200, 1373, 1589
FatI CATG 7 cut(s) 255, 364, 469, 892, 980, 1091, 1430
FauI CCCGC 1 cut(s) 573
FblI GTMKAC 1 cut(s) 824
Fnu4HI GCNGC 6 cut(s) 37, 252, 714, 1410, 1473, 1632
FokI GGATG 8 cut(s) 68, 272, 638, 748, 1361, 1413, 1594, 1633
Fsp4HI GCNGC 6 cut(s) 37, 252, 714, 1410, 1473, 1632
FspBI CTAG 3 cut(s) 329, 828, 1239
GlaI GCGC 1 cut(s) 80
GluI GCNGC 6 cut(s) 37, 252, 714, 1410, 1473, 1632
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 1 cut(s) 184
HaeIII GGCC 5 cut(s) 159, 254, 868, 1297, 1402
HapII CCGG 2 cut(s) 76, 430
HgaI GACGC 1 cut(s) 415
HhaI GCGC 1 cut(s) 81
Hin1II CATG 7 cut(s) 259, 368, 473, 896, 984, 1095, 1434
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 5 cut(s) 28, 102, 635, 670, 1583
HpaII CCGG 2 cut(s) 76, 430
HphI GGTGA 7 cut(s) 314, 331, 404, 1054, 1130, 1157, 1274
Hpy166II GTNNAC 5 cut(s) 405, 825, 1002, 1495, 1619
Hpy188III TCNNGA 8 cut(s) 118, 201, 274, 329, 588, 719, 1037, 1239
Hpy8I GTNNAC 5 cut(s) 405, 825, 1002, 1495, 1619
Hpy99I CGWCG 3 cut(s) 23, 52, 1284
HpyAV CCTTC 2 cut(s) 53, 1631
HpyCH4III ACNGT 8 cut(s) 129, 172, 999, 1026, 1142, 1262, 1416, 1499
HpyCH4IV ACGT 2 cut(s) 47, 989
HpyF10VI GCNNNNNNNGC 3 cut(s) 502, 710, 1294
HpyF3I CTNAG 6 cut(s) 246, 768, 950, 1174, 1222, 1392
HpySE526I ACGT 2 cut(s) 47, 989
Hsp92II CATG 7 cut(s) 259, 368, 473, 896, 984, 1095, 1434
HspAI GCGC 1 cut(s) 79
Kzo9I GATC 8 cut(s) 22, 574, 848, 1044, 1075, 1132, 1168, 1434
LmnI GCTCC 1 cut(s) 1189
Lsp1109I GCAGC 5 cut(s) 48, 725, 1396, 1459, 1643
LweI GCATC 8 cut(s) 616, 638, 736, 788, 847, 947, 1194, 1333
MaeI CTAG 3 cut(s) 329, 828, 1239
MaeII ACGT 2 cut(s) 47, 989
MaeIII GTNAC 2 cut(s) 1145, 1499
MalI GATC 8 cut(s) 24, 576, 850, 1046, 1077, 1134, 1170, 1436
MbiI CCGCTC 2 cut(s) 123, 568
MboI GATC 8 cut(s) 22, 574, 848, 1044, 1075, 1132, 1168, 1434
MboII GAAGA 5 cut(s) 596, 694, 708, 827, 1523
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 2 cut(s) 574, 1075
MhlI GDGCHC 1 cut(s) 595
MluCI AATT 5 cut(s) 942, 959, 1328, 1352, 1576
MlyI GAGTC 3 cut(s) 37, 644, 679
MmeI TCCRAC 5 cut(s) 30, 460, 602, 653, 965
Mph1103I ATGCAT 3 cut(s) 631, 982, 1097
MseI TTAA 7 cut(s) 339, 414, 453, 656, 1251, 1355, 1575
MslI CAYNNNNRTG 1 cut(s) 1143
MspA1I CMGCKG 1 cut(s) 505
MspI CCGG 2 cut(s) 76, 430
MspR9I CCNGG 7 cut(s) 184, 300, 431, 609, 885, 1628, 1651
MunI CAATTG 1 cut(s) 942
Mva1269I GAATGC 3 cut(s) 343, 441, 982
MvaI CCWGG 6 cut(s) 184, 300, 609, 885, 1628, 1651
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 3 cut(s) 502, 710, 1294
NciI CCSGG 1 cut(s) 431
NcoI CCATGG 2 cut(s) 255, 1430
NdeII GATC 8 cut(s) 22, 574, 848, 1044, 1075, 1132, 1168, 1434
NlaIII CATG 7 cut(s) 259, 368, 473, 896, 984, 1095, 1434
NlaIV GGNNCC 7 cut(s) 64, 158, 351, 576, 677, 1298, 1461
NmeAIII GCCGAG 4 cut(s) 129, 1029, 1049, 1429
NmuCI GTSAC 2 cut(s) 1145, 1499
NsiI ATGCAT 3 cut(s) 631, 982, 1097
NspI RCATGY 3 cut(s) 368, 984, 1095
OliI CACNNNNGTG 1 cut(s) 1143
PaeI GCATGC 1 cut(s) 1095
PciI ACATGT 1 cut(s) 364
PcsI WCGNNNNNNNCGW 1 cut(s) 1285
PctI GAATGC 3 cut(s) 343, 441, 982
PfeI GAWTC 2 cut(s) 102, 1583
PflFI GACNNNGTC 2 cut(s) 583, 1385
PflMI CCANNNNNTGG 1 cut(s) 174
PfoI TCCNGGA 1 cut(s) 883
PkrI GCNGC 6 cut(s) 38, 253, 715, 1411, 1474, 1633
Ple19I CGATCG 1 cut(s) 25
PleI GAGTC 3 cut(s) 36, 643, 678
PpsI GAGTC 3 cut(s) 36, 643, 678
PpuMI RGGWCCY 1 cut(s) 1445
PscI ACATGT 1 cut(s) 364
PshAI GACNNNNGTC 1 cut(s) 505
PshBI ATTAAT 1 cut(s) 1575
Psp5II RGGWCCY 1 cut(s) 1445
Psp6I CCWGG 6 cut(s) 182, 298, 607, 883, 1626, 1649
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 6 cut(s) 182, 298, 607, 883, 1626, 1649
PspN4I GGNNCC 7 cut(s) 64, 158, 351, 576, 677, 1298, 1461
PspPI GGNCC 5 cut(s) 157, 350, 866, 1296, 1445
PspPPI RGGWCCY 1 cut(s) 1445
PsrI GAACNNNNNNTAC 2 cut(s) 983, 1015
PstNI CAGNNNCTG 1 cut(s) 860
PsuI RGATCY 2 cut(s) 574, 1075
PsyI GACNNNGTC 2 cut(s) 583, 1385
PvuI CGATCG 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 505
RsaI GTAC 4 cut(s) 386, 992, 1125, 1325
RsaNI GTAC 4 cut(s) 385, 991, 1124, 1324
RseI CAYNNNNRTG 1 cut(s) 1143
SaqAI TTAA 7 cut(s) 339, 414, 453, 656, 1251, 1355, 1575
SatI GCNGC 6 cut(s) 37, 252, 714, 1410, 1473, 1632
Sau3AI GATC 8 cut(s) 22, 574, 848, 1044, 1075, 1132, 1168, 1434
Sau96I GGNCC 5 cut(s) 157, 350, 866, 1296, 1445
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 3 cut(s) 37, 644, 679
ScrFI CCNGG 7 cut(s) 184, 300, 431, 609, 885, 1628, 1651
SduI GDGCHC 1 cut(s) 595
SfaNI GCATC 8 cut(s) 616, 638, 736, 788, 847, 947, 1194, 1333
SfcI CTRYAG 1 cut(s) 1467
SinI GGWCC 2 cut(s) 350, 1445
SmiMI CAYNNNNRTG 1 cut(s) 1143
SmlI CTYRAG 2 cut(s) 569, 717
SmoI CTYRAG 2 cut(s) 569, 717
SpeI ACTAGT 1 cut(s) 827
SphI GCATGC 1 cut(s) 1095
Sse9I AATT 5 cut(s) 942, 959, 1328, 1352, 1576
SsiI CCGC 4 cut(s) 81, 123, 251, 566
SspI AATATT 1 cut(s) 1061
SspMI CTAG 3 cut(s) 329, 828, 1239
StyD4I CCNGG 7 cut(s) 182, 298, 429, 607, 883, 1626, 1649
StyI CCWWGG 2 cut(s) 255, 1430
TaaI ACNGT 8 cut(s) 129, 172, 999, 1026, 1142, 1262, 1416, 1499
TaiI ACGT 2 cut(s) 50, 992
TaqI TCGA 3 cut(s) 509, 1231, 1380
TasI AATT 5 cut(s) 942, 959, 1328, 1352, 1576
TatI WGTACW 2 cut(s) 384, 1123
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 2 cut(s) 102, 1583
Tru1I TTAA 7 cut(s) 339, 414, 453, 656, 1251, 1355, 1575
Tru9I TTAA 7 cut(s) 339, 414, 453, 656, 1251, 1355, 1575
TscAI CASTG 6 cut(s) 250, 526, 772, 1147, 1421, 1504
TseFI GTSAC 2 cut(s) 1145, 1499
TseI GCWGC 5 cut(s) 36, 713, 1409, 1472, 1631
Tsp45I GTSAC 2 cut(s) 1145, 1499
TspDTI ATGAA 3 cut(s) 72, 753, 1415
TspRI CASTG 6 cut(s) 250, 526, 772, 1147, 1421, 1504
Tth111I GACNNNGTC 2 cut(s) 583, 1385
Van91I CCANNNNNTGG 1 cut(s) 174
VpaK11BI GGWCC 2 cut(s) 350, 1445
VspI ATTAAT 1 cut(s) 1575
XagI CCTNNNNNAGG 1 cut(s) 1304
XapI RAATTY 1 cut(s) 1328
XbaI TCTAGA 2 cut(s) 328, 1238
XceI RCATGY 3 cut(s) 368, 984, 1095
XmiI GTMKAC 1 cut(s) 824
XspI CTAG 3 cut(s) 329, 828, 1239
ZrmI AGTACT 1 cut(s) 386
Zsp2I ATGCAT 3 cut(s) 631, 982, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.