pycom12g09920

SRSF protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008320
Physical Location & Seq
Forward (+)
11794680 .. 11797660
2981 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g09920.4

Sequence Viewer

Length: 1665 bp
ATGGGGGATAAACAGGACGACGATCGGAGTCAGAGCAGCGACTACACGTCGGAGGACGAAGGCACCGAGGACTACCGGCGCGGCGGCTACCACGCCGTCCGAATCGGCGACACTTTCAAGAGCGGACGGTATGTCGTTCAGACCAAGCTCGGCTGGGGCCATTTCTCCACCGTCTGGCTCGCCTGGGACACCCAACACTCCAGATTTGTTGCTCTGAAAGTGCAAAAGAGTGCTGACCACTACACCGAGGCGGCCATGGATGAGATAACCATCTTGAAACAGATTGCAGAGGGAGACCCAGATGATAAAAAATGCGTGGTGAAGCTTCTAGACCATTTTAAGCATTCGGGTCCGAATGGGCAACATGTTTGTATGGTTTTTGAGTACTTGGGTGACAATCTGTTGACGCTTATTAAGTATAGTGATTACCGGGGAGTGCCACTACATAAGGTTAAGGAGATTTGTTATCATGTTTTGGTTGGATTGGATTATTTGCACAGACAGCTGTCGATTATACACACTGATTTGAAGCCAGAGAATATCTTGTTGATGTCAATGATAGACCCGCTCAAGGATCCGACAAAGTCAAGAGCACCTCTTATTCTTCCGGGTGGTAACGACAAGGATGCATCGGAGTCAGGGTATGCAAAAGTGTTAAATGGGGATATGAGTCGGAACCAGAAGAAAAAGATTAGAAGAAAGGCGAAGCGTGCAGCTCAAGAGTGCGTGGATAGAGATGAAGCTGATGCTGATGTAGAAACATCCACTGAGGTAGAGGCATCTCCTAACACAAGATTGAATGTGCGTTCTGTCGAAGACCAGTCTACTAGTTCTGTCAGTACGAATAGATCATCTGGCCCCAGAGATGCTGGTGGGCCAAATGGCTCTCGTCCAGGAAGTCAGGGTAGTAAGAGAGGCAATCGCACCACAAGGCAGAAGTTGTTGGCATCAATTGACCTGACGTGCAAATTGGTTGATTTCGGGAATGCATGTTGGACGTACAAACAGTTCACAAACGATATCCAAACAAGACAGTATAGGTGTCCAGAGGTGATCCTCGGCTCAAAATATTCAACCTCGGCAGATCTTTGGTCTTTTGCATGCATTTGTTTTGAGCTTGCAACGGGTGATGTACTCTTTGATCCCCACAGCGGTGACAACTTTGACAGGGATGAGGATCACTTAGCATTGATGATGGAGCTTCTTGGAATGATGCCACGAAAGATTGCCTTAGGTGGCCGATATTCTAGAGATTACTTTAATAGATACGGGGATTTGAGGCACATTCGTCGCTTGCGTTTTTGGCCCCTGAATAAGGTTCTGGTAGAAAAGTACGAATTCAGCGAGAAAGATGCAAGTGAATTAACCGACTTCCTCGTTCCCATCCTCGACTTTGTCCCTGAGAAACGGCCTACTGCTGCACAGTGCCTTCTTCATCCATGGATCAATGCAGGTCCTCGGCTACTGGAACCATCTATAGCTGCTGATAAAAAACAAGGAGTGGGCAGTGACACGGCTGAAGAAAACCAGAGGGAAAAAGACGAGAGGGAGGCAATGGAGGTTGGAGTGGGAAATATCGCCATTAATTCCGATTCTAAACGGGTCAAAGATGTCCCATCCAGTAGTAAACCTTCCAGGGCAGCCGAAGGTAATCCATCCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000245 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016071 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016779 GO:0019219 GO:0019222 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0023052 GO:0031323 GO:0031325 GO:0032153 GO:0032386 GO:0032388 GO:0032507 GO:0032879 GO:0032880 GO:0033036 GO:0033120 GO:0033157 GO:0033365 GO:0034504 GO:0034613 GO:0034622 GO:0034641 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045185 GO:0045935 GO:0046483 GO:0046822 GO:0046824 GO:0046825 GO:0046827 GO:0048024 GO:0048026 GO:0048518 GO:0048522 GO:0050265 GO:0050684 GO:0050685 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051235 GO:0051252 GO:0051254 GO:0051457 GO:0051641 GO:0051651 GO:0051716 GO:0060255 GO:0060341 GO:0065003 GO:0065007 GO:0065008 GO:0070201 GO:0070569 GO:0070727 GO:0071704 GO:0071826 GO:0071840 GO:0072595 GO:0080090 GO:0090087 GO:0090304 GO:0090316 GO:0140096 GO:0140098 GO:1901360 GO:1901564 GO:1903311 GO:1903313 GO:1903827 GO:1903829 GO:1904951
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

555

Amino Acids

62.31

Weight (kDa)

6.46

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1442
AccB1I GGYRCC 1 cut(s) 62
AccB7I CCANNNNNTGG 1 cut(s) 174
AccBSI CCGCTC 2 cut(s) 123, 568
AccI GTMKAC 1 cut(s) 824
AccII CGCG 1 cut(s) 81
AciI CCGC 6 cut(s) 81, 84, 123, 251, 566, 1152
AclWI GGATC 6 cut(s) 569, 582, 1048, 1136, 1185, 1451
AcoI YGGCCR 2 cut(s) 252, 1237
AcsI RAATTY 1 cut(s) 1337
AcuI CTGAAG 1 cut(s) 1539
AfaI GTAC 5 cut(s) 386, 841, 1001, 1134, 1334
AfiI CCNNNNNNNGG 4 cut(s) 174, 571, 1151, 1315
AflIII ACRYGT 2 cut(s) 45, 364
AgsI TTSAA 5 cut(s) 118, 277, 529, 799, 1074
AhdI GACNNNNNGTC 2 cut(s) 46, 131
AhlI ACTAGT 1 cut(s) 827
AjiI CACGTC 2 cut(s) 48, 963
AjnI CCWGG 4 cut(s) 182, 892, 1634, 1658
AleI CACNNNNGTG 1 cut(s) 1152
AluBI AGCT 8 cut(s) 148, 325, 505, 716, 743, 1117, 1201, 1481
AluI AGCT 8 cut(s) 148, 325, 505, 716, 743, 1117, 1201, 1481
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 6 cut(s) 569, 582, 1048, 1136, 1185, 1451
AoxI GGCC 7 cut(s) 157, 252, 856, 875, 1237, 1304, 1409
ApeKI GCWGC 5 cut(s) 36, 713, 1418, 1481, 1640
ApoI RAATTY 1 cut(s) 1337
AseI ATTAAT 1 cut(s) 1584
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 6 cut(s) 157, 350, 857, 875, 1305, 1454
AsuC2I CCSGG 2 cut(s) 431, 609
AsuHPI GGTGA 5 cut(s) 331, 404, 1063, 1139, 1166
AvaII GGWCC 2 cut(s) 350, 1454
AxyI CCTNAGG 1 cut(s) 1231
BamHI GGATCC 1 cut(s) 574
BanI GGYRCC 1 cut(s) 62
BarI GAAGNNNNNNTAC 2 cut(s) 1615, 1647
BbsI GAAGAC 1 cut(s) 822
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 5 cut(s) 48, 725, 1405, 1468, 1652
BccI CCATC 6 cut(s) 278, 1189, 1391, 1480, 1624, 1663
BceAI ACGGC 3 cut(s) 80, 1424, 1530
BcgI CGANNNNNNTGC 6 cut(s) 608, 642, 1271, 1305, 1334, 1368
BciT130I CCWGG 4 cut(s) 184, 894, 1636, 1660
BcnI CCSGG 2 cut(s) 431, 609
BcoDI GTCTC 1 cut(s) 288
BcuI ACTAGT 1 cut(s) 827
BfaI CTAG 3 cut(s) 329, 828, 1248
BfmI CTRYAG 1 cut(s) 1476
BfuAI ACCTGC 1 cut(s) 1442
BglII AGATCT 1 cut(s) 1084
BisI GCNGC 8 cut(s) 37, 82, 85, 252, 714, 1419, 1482, 1641
BlsI GCNGC 8 cut(s) 38, 83, 86, 253, 715, 1420, 1483, 1642
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 6 cut(s) 184, 431, 609, 894, 1636, 1660
Bme18I GGWCC 2 cut(s) 350, 1454
BmeRI GACNNNNNGTC 2 cut(s) 46, 131
BmgBI CACGTC 2 cut(s) 48, 963
BmgT120I GGNCC 6 cut(s) 157, 350, 857, 875, 1305, 1454
BmiI GGNNCC 8 cut(s) 64, 158, 351, 576, 677, 859, 1307, 1470
BmrFI CCNGG 6 cut(s) 184, 431, 609, 894, 1636, 1660
BmsI GCATC 8 cut(s) 616, 638, 736, 788, 856, 956, 1203, 1342
BoxI GACNNNNGTC 1 cut(s) 505
BpiI GAAGAC 1 cut(s) 822
BpmI CTGGAG 1 cut(s) 184
BpuEI CTTGAG 2 cut(s) 554, 702
BpuMI CCSGG 2 cut(s) 431, 609
BsaBI GATNNNNATC 2 cut(s) 269, 1176
BsaI GGTCTC 1 cut(s) 288
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 571, 1151, 1315
Bse118I RCCGGY 1 cut(s) 75
Bse1I ACTGG 3 cut(s) 820, 1470, 1620
Bse21I CCTNAGG 1 cut(s) 1231
Bse3DI GCAATG 1 cut(s) 1560
Bse8I GATNNNNATC 2 cut(s) 269, 1176
BseBI CCWGG 4 cut(s) 184, 894, 1636, 1660
BseGI GGATG 8 cut(s) 265, 631, 761, 1177, 1383, 1435, 1616, 1655
BseJI GATNNNNATC 2 cut(s) 269, 1176
BseLI CCNNNNNNNGG 4 cut(s) 174, 571, 1151, 1315
BseMI GCAATG 1 cut(s) 1560
BseMII CTCAG 2 cut(s) 759, 1392
BseNI ACTGG 3 cut(s) 820, 1470, 1620
BseXI GCAGC 5 cut(s) 48, 725, 1405, 1468, 1652
BseYI CCCAGC 1 cut(s) 153
BsgI GTGCAG 2 cut(s) 732, 1404
Bsh1236I CGCG 1 cut(s) 81
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 7 cut(s) 159, 254, 858, 877, 1239, 1306, 1411
BshNI GGYRCC 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 595
BsiSI CCGG 3 cut(s) 76, 430, 608
BslFI GGGAC 3 cut(s) 200, 1382, 1598
BslI CCNNNNNNNGG 4 cut(s) 174, 571, 1151, 1315
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 3 cut(s) 200, 1382, 1598
BsmI GAATGC 2 cut(s) 343, 991
BsnI GGCC 7 cut(s) 159, 254, 858, 877, 1239, 1306, 1411
Bso31I GGTCTC 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 595
Bsp143I GATC 8 cut(s) 22, 574, 848, 1053, 1084, 1141, 1177, 1443
Bsp19I CCATGG 2 cut(s) 255, 1439
BspACI CCGC 6 cut(s) 81, 84, 123, 251, 566, 1152
BspANI GGCC 7 cut(s) 159, 254, 858, 877, 1239, 1306, 1411
BspCNI CTCAG 2 cut(s) 760, 1393
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 8 cut(s) 64, 158, 351, 576, 677, 859, 1307, 1470
BspMI ACCTGC 1 cut(s) 1442
BspPI GGATC 6 cut(s) 569, 582, 1048, 1136, 1185, 1451
BspT107I GGYRCC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 288
BsrBI CCGCTC 2 cut(s) 123, 568
BsrDI GCAATG 1 cut(s) 1560
BsrFI RCCGGY 1 cut(s) 75
BsrI ACTGG 3 cut(s) 820, 1470, 1620
BssAI RCCGGY 1 cut(s) 75
BssMI GATC 8 cut(s) 22, 574, 848, 1053, 1084, 1141, 1177, 1443
BssT1I CCWWGG 2 cut(s) 255, 1439
Bst2UI CCWGG 4 cut(s) 184, 894, 1636, 1660
Bst4CI ACNGT 5 cut(s) 129, 172, 1008, 1035, 1425
BstC8I GCNNGC 5 cut(s) 180, 711, 1102, 1119, 1295
BstDEI CTNAG 4 cut(s) 768, 1183, 1231, 1401
BstDSI CCRYGG 2 cut(s) 255, 1439
BstENI CCTNNNNNAGG 1 cut(s) 1313
BstF5I GGATG 8 cut(s) 265, 631, 761, 1177, 1383, 1435, 1616, 1655
BstFNI CGCG 1 cut(s) 81
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 8 cut(s) 25, 577, 851, 1056, 1087, 1144, 1180, 1446
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 8 cut(s) 22, 574, 848, 1053, 1084, 1141, 1177, 1443
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 3 cut(s) 502, 710, 1303
BstNI CCWGG 4 cut(s) 184, 894, 1636, 1660
BstNSI RCATGY 3 cut(s) 368, 993, 1104
BstPAI GACNNNNGTC 1 cut(s) 505
BstSCI CCNGG 6 cut(s) 182, 429, 607, 892, 1634, 1658
BstSFI CTRYAG 1 cut(s) 1476
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 5 cut(s) 48, 725, 1405, 1468, 1652
BstV2I GAAGAC 1 cut(s) 822
BstX2I RGATCY 2 cut(s) 574, 1084
BstYI RGATCY 2 cut(s) 574, 1084
Bsu36I CCTNAGG 1 cut(s) 1231
BsuRI GGCC 7 cut(s) 159, 254, 858, 877, 1239, 1306, 1411
BtgI CCRYGG 2 cut(s) 255, 1439
BtrI CACGTC 2 cut(s) 48, 963
BtsCI GGATG 8 cut(s) 265, 631, 761, 1177, 1383, 1435, 1616, 1655
BtsI GCAGTG 1 cut(s) 1513
BtsIMutI CAGTG 4 cut(s) 519, 765, 1430, 1513
BveI ACCTGC 1 cut(s) 1442
Cac8I GCNNGC 5 cut(s) 180, 711, 1102, 1119, 1295
CfoI GCGC 1 cut(s) 81
Cfr10I RCCGGY 1 cut(s) 75
Cfr13I GGNCC 6 cut(s) 157, 350, 857, 875, 1305, 1454
CseI GACGC 1 cut(s) 415
Csp6I GTAC 5 cut(s) 385, 840, 1000, 1133, 1333
CviAII CATG 6 cut(s) 256, 365, 470, 990, 1101, 1440
CviQI GTAC 5 cut(s) 385, 840, 1000, 1133, 1333
DdeI CTNAG 4 cut(s) 768, 1183, 1231, 1401
DpnI GATC 8 cut(s) 24, 576, 850, 1055, 1086, 1143, 1179, 1445
DpnII GATC 8 cut(s) 22, 574, 848, 1053, 1084, 1141, 1177, 1443
DriI GACNNNNNGTC 2 cut(s) 46, 131
EaeI YGGCCR 2 cut(s) 252, 1237
Eam1105I GACNNNNNGTC 2 cut(s) 46, 131
Eco130I CCWWGG 2 cut(s) 255, 1439
Eco31I GGTCTC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 1021
Eco47I GGWCC 2 cut(s) 350, 1454
Eco57I CTGAAG 1 cut(s) 1539
Eco81I CCTNAGG 1 cut(s) 1231
EcoNI CCTNNNNNAGG 1 cut(s) 1313
EcoO109I RGGNCCY 1 cut(s) 1454
EcoRI GAATTC 1 cut(s) 1337
EcoRII CCWGG 4 cut(s) 182, 892, 1634, 1658
EcoRV GATATC 1 cut(s) 1021
EcoT14I CCWWGG 2 cut(s) 255, 1439
EcoT22I ATGCAT 3 cut(s) 631, 991, 1106
ErhI CCWWGG 2 cut(s) 255, 1439
FaeI CATG 6 cut(s) 259, 368, 473, 993, 1104, 1443
FalI AAGNNNNNCTT 2 cut(s) 1214, 1246
FaqI GGGAC 3 cut(s) 200, 1382, 1598
FatI CATG 6 cut(s) 255, 364, 469, 989, 1100, 1439
FauI CCCGC 1 cut(s) 573
FblI GTMKAC 1 cut(s) 824
Fnu4HI GCNGC 8 cut(s) 37, 82, 85, 252, 714, 1419, 1482, 1641
FokI GGATG 8 cut(s) 272, 638, 748, 1184, 1370, 1422, 1603, 1642
Fsp4HI GCNGC 8 cut(s) 37, 82, 85, 252, 714, 1419, 1482, 1641
FspBI CTAG 3 cut(s) 329, 828, 1248
GlaI GCGC 1 cut(s) 80
GluI GCNGC 8 cut(s) 37, 82, 85, 252, 714, 1419, 1482, 1641
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 1 cut(s) 184
HaeIII GGCC 7 cut(s) 159, 254, 858, 877, 1239, 1306, 1411
HapII CCGG 3 cut(s) 76, 430, 608
HgaI GACGC 1 cut(s) 415
HhaI GCGC 1 cut(s) 81
Hin1II CATG 6 cut(s) 259, 368, 473, 993, 1104, 1443
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 5 cut(s) 28, 102, 635, 670, 1592
HpaII CCGG 3 cut(s) 76, 430, 608
HphI GGTGA 5 cut(s) 331, 404, 1063, 1139, 1166
Hpy166II GTNNAC 4 cut(s) 405, 825, 1011, 1628
Hpy188III TCNNGA 9 cut(s) 118, 201, 274, 329, 588, 719, 982, 1046, 1248
Hpy8I GTNNAC 4 cut(s) 405, 825, 1011, 1628
Hpy99I CGWCG 3 cut(s) 23, 52, 1293
HpyAV CCTTC 4 cut(s) 53, 1439, 1640, 1641
HpyCH4III ACNGT 5 cut(s) 129, 172, 1008, 1035, 1425
HpyCH4IV ACGT 3 cut(s) 47, 962, 998
HpyF10VI GCNNNNNNNGC 3 cut(s) 502, 710, 1303
HpyF3I CTNAG 4 cut(s) 768, 1183, 1231, 1401
HpySE526I ACGT 3 cut(s) 47, 962, 998
Hsp92II CATG 6 cut(s) 259, 368, 473, 993, 1104, 1443
HspAI GCGC 1 cut(s) 79
Kzo9I GATC 8 cut(s) 22, 574, 848, 1053, 1084, 1141, 1177, 1443
LmnI GCTCC 1 cut(s) 1198
Lsp1109I GCAGC 5 cut(s) 48, 725, 1405, 1468, 1652
LweI GCATC 8 cut(s) 616, 638, 736, 788, 856, 956, 1203, 1342
MaeI CTAG 3 cut(s) 329, 828, 1248
MaeII ACGT 3 cut(s) 47, 962, 998
MaeIII GTNAC 4 cut(s) 392, 614, 1154, 1508
MalI GATC 8 cut(s) 24, 576, 850, 1055, 1086, 1143, 1179, 1445
MbiI CCGCTC 2 cut(s) 123, 568
MboI GATC 8 cut(s) 22, 574, 848, 1053, 1084, 1141, 1177, 1443
MboII GAAGA 6 cut(s) 596, 694, 708, 827, 1424, 1532
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 2 cut(s) 574, 1084
MhlI GDGCHC 1 cut(s) 595
MluCI AATT 5 cut(s) 951, 968, 1337, 1361, 1585
MlyI GAGTC 3 cut(s) 37, 644, 679
MmeI TCCRAC 6 cut(s) 30, 460, 602, 653, 974, 1543
Mph1103I ATGCAT 3 cut(s) 631, 991, 1106
MseI TTAA 7 cut(s) 339, 414, 453, 656, 1260, 1364, 1584
MslI CAYNNNNRTG 1 cut(s) 1152
MspA1I CMGCKG 2 cut(s) 505, 1152
MspI CCGG 3 cut(s) 76, 430, 608
MspR9I CCNGG 6 cut(s) 184, 431, 609, 894, 1636, 1660
MunI CAATTG 1 cut(s) 951
Mva1269I GAATGC 2 cut(s) 343, 991
MvaI CCWGG 4 cut(s) 184, 894, 1636, 1660
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 3 cut(s) 502, 710, 1303
NciI CCSGG 2 cut(s) 431, 609
NcoI CCATGG 2 cut(s) 255, 1439
NdeII GATC 8 cut(s) 22, 574, 848, 1053, 1084, 1141, 1177, 1443
NlaIII CATG 6 cut(s) 259, 368, 473, 993, 1104, 1443
NlaIV GGNNCC 8 cut(s) 64, 158, 351, 576, 677, 859, 1307, 1470
NmeAIII GCCGAG 4 cut(s) 129, 1038, 1058, 1438
NmuCI GTSAC 3 cut(s) 392, 1154, 1508
NsiI ATGCAT 3 cut(s) 631, 991, 1106
NspI RCATGY 3 cut(s) 368, 993, 1104
OliI CACNNNNGTG 1 cut(s) 1152
PaeI GCATGC 1 cut(s) 1104
PciI ACATGT 1 cut(s) 364
PcsI WCGNNNNNNNCGW 3 cut(s) 63, 1294, 1341
PctI GAATGC 2 cut(s) 343, 991
PfeI GAWTC 2 cut(s) 102, 1592
PflFI GACNNNGTC 2 cut(s) 583, 1394
PflMI CCANNNNNTGG 1 cut(s) 174
PfoI TCCNGGA 1 cut(s) 892
PkrI GCNGC 8 cut(s) 38, 83, 86, 253, 715, 1420, 1483, 1642
Ple19I CGATCG 1 cut(s) 25
PleI GAGTC 3 cut(s) 36, 643, 678
PpsI GAGTC 3 cut(s) 36, 643, 678
PpuMI RGGWCCY 1 cut(s) 1454
PscI ACATGT 1 cut(s) 364
PshAI GACNNNNGTC 1 cut(s) 505
PshBI ATTAAT 1 cut(s) 1584
Psp5II RGGWCCY 1 cut(s) 1454
Psp6I CCWGG 4 cut(s) 182, 892, 1634, 1658
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 4 cut(s) 182, 892, 1634, 1658
PspN4I GGNNCC 8 cut(s) 64, 158, 351, 576, 677, 859, 1307, 1470
PspPI GGNCC 6 cut(s) 157, 350, 857, 875, 1305, 1454
PspPPI RGGWCCY 1 cut(s) 1454
PsrI GAACNNNNNNTAC 2 cut(s) 992, 1024
PsuI RGATCY 2 cut(s) 574, 1084
PsyI GACNNNGTC 2 cut(s) 583, 1394
PvuI CGATCG 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 505
RsaI GTAC 5 cut(s) 386, 841, 1001, 1134, 1334
RsaNI GTAC 5 cut(s) 385, 840, 1000, 1133, 1333
RseI CAYNNNNRTG 1 cut(s) 1152
SaqAI TTAA 7 cut(s) 339, 414, 453, 656, 1260, 1364, 1584
SatI GCNGC 8 cut(s) 37, 82, 85, 252, 714, 1419, 1482, 1641
Sau3AI GATC 8 cut(s) 22, 574, 848, 1053, 1084, 1141, 1177, 1443
Sau96I GGNCC 6 cut(s) 157, 350, 857, 875, 1305, 1454
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 3 cut(s) 37, 644, 679
ScrFI CCNGG 6 cut(s) 184, 431, 609, 894, 1636, 1660
SduI GDGCHC 1 cut(s) 595
SfaNI GCATC 8 cut(s) 616, 638, 736, 788, 856, 956, 1203, 1342
SfcI CTRYAG 1 cut(s) 1476
SinI GGWCC 2 cut(s) 350, 1454
SmiMI CAYNNNNRTG 1 cut(s) 1152
SmlI CTYRAG 2 cut(s) 569, 717
SmoI CTYRAG 2 cut(s) 569, 717
SpeI ACTAGT 1 cut(s) 827
SphI GCATGC 1 cut(s) 1104
Sse9I AATT 5 cut(s) 951, 968, 1337, 1361, 1585
SsiI CCGC 6 cut(s) 81, 84, 123, 251, 566, 1152
SspI AATATT 1 cut(s) 1070
SspMI CTAG 3 cut(s) 329, 828, 1248
StyD4I CCNGG 6 cut(s) 182, 429, 607, 892, 1634, 1658
StyI CCWWGG 2 cut(s) 255, 1439
TaaI ACNGT 5 cut(s) 129, 172, 1008, 1035, 1425
TaiI ACGT 3 cut(s) 50, 965, 1001
TaqI TCGA 3 cut(s) 509, 813, 1389
TasI AATT 5 cut(s) 951, 968, 1337, 1361, 1585
TatI WGTACW 2 cut(s) 384, 1132
TauI GCSGC 3 cut(s) 84, 87, 254
TfiI GAWTC 2 cut(s) 102, 1592
Tru1I TTAA 7 cut(s) 339, 414, 453, 656, 1260, 1364, 1584
Tru9I TTAA 7 cut(s) 339, 414, 453, 656, 1260, 1364, 1584
TscAI CASTG 4 cut(s) 526, 772, 1430, 1513
TseFI GTSAC 3 cut(s) 392, 1154, 1508
TseI GCWGC 5 cut(s) 36, 713, 1418, 1481, 1640
Tsp45I GTSAC 3 cut(s) 392, 1154, 1508
TspDTI ATGAA 2 cut(s) 753, 1424
TspRI CASTG 4 cut(s) 526, 772, 1430, 1513
Tth111I GACNNNGTC 2 cut(s) 583, 1394
Van91I CCANNNNNTGG 1 cut(s) 174
VpaK11BI GGWCC 2 cut(s) 350, 1454
VspI ATTAAT 1 cut(s) 1584
XagI CCTNNNNNAGG 1 cut(s) 1313
XapI RAATTY 1 cut(s) 1337
XbaI TCTAGA 2 cut(s) 328, 1247
XceI RCATGY 3 cut(s) 368, 993, 1104
XmiI GTMKAC 1 cut(s) 824
XspI CTAG 3 cut(s) 329, 828, 1248
ZrmI AGTACT 1 cut(s) 386
Zsp2I ATGCAT 3 cut(s) 631, 991, 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.