pycom14g09320

SRSF protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008320
Physical Location & Seq
Reverse (-)
11143030 .. 11145968
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g09320.2

Sequence Viewer

Length: 1656 bp
ATGGGGGATAACCAGGACGACGATCGGAGTCAGAGCAGCGACTACACGTCGGCGGACGAAGGCACCGAAGACTACCGGCGCGGCGGCTACCACGCCGTTCGAATCGGCGACACTTTCAAGAGCGGACGGTATGTCGTTCAGACCAAGCTCGGTTGGGGCCATTTCTCCACCGTCTGGCTCGCCTGGGACACCCAACACTCTAGGTTTGTTGCTCTGAAAGTGCAAAAGAGTGCTGACCACTATACGGAGGCGGCCATGGATGAGATAACCATCTTGAAACAGATTGCAGAGGGAGACCCGGATGATAAAAAATGCGTGGTGAAGCTTCTGGACCATTTTAAGCATTCGGGACCAAATGGGCAGCATGTTTGTATGGTTTTCGAGTACTTGGGTGATAATCTGTTGACGCTTATTAAGTACAGTGATTACCGGGGAGTGCCATTACGTATGGTTAAGGAGATTTGTTATCATGTTTTGGTTGGATTGGATTATTTGCACAGACAGCTCTCCATTATACACACTGATTTGAAGCCGGAGAATATCTTGTTGATGTCAATGATAGACCATCTCAAGGATCCGACAAATTCAAGAGCACCTCTTATTCTTCCAAGTGGTAAAGACAAGGATTCATCGGAGTCGGGGTATGCAAAAGCATTAAATGGGGATGTGTCTCGGAACCAGAAGAAAAAGATTAGAAGAAAGGCAAAGCGTGCAGCTCAAGAGTGCGCGGATAGAGATGAAGCTGATGCTGATGTAGAAACATCCGCCAAGGTAGAGGCATCTCTTAACACAAGATTGAATGTGTGTTCTGTCGAAGAGCAGACTACTAGTTCTGTCAAAGCGGATAGATCATCAGATGCTGATGGACCAAATGGATCTCGTCCAGGAAGTCAGGGTACTAAGAGAGGCAATCGCACCACAAGGCGGAAGTTGTTGGCATCAATTGACCTGAAGTGCAAATTGGTTGATTTTGGTAATGCATGTTGGACGTACAAACAGTTCACAAACGATATCCAAACAAGACAGTATAGGTGCCCAGAGGTGATCCTCGGCTCAAAATATTCTACCTCCGCAGATCTTTGGTCTTTTGCATGCATTTGTTTTGAGCTTGCGACTGGTGATGTGCTCTTTGATCCCCACAGTGGTGACAACTTTGATAGGGATGAGGATCACTTAGCATTGATGATGGAGCTTCTTGGAATGATGCCACGAAAGATTGCCTTAGGTGGTCGATATTCCAGAGATTACTTTAATAGATATGGTGATTTGAGGCACATTCGCCGCTTGCGCTTTTGGCCCCTGAATAAGGTTCTGGTAGAAAAGTATGAATTCAGCGAGAAAGATGCAAGTGAATTGGCTGACTTTCTCGTTACCATCCTCGACTTTGTCCCTGAGAAACGGCCTACTGCTGCACAGTGCCTTCTTCATCCATGGATCAGTGCAGGTCCTCGACTATTGGAACCATCTATAGCTTCTGATAAAAAGCAAGGAGTGGACGGTGACACGACTGAAGAAAACCAGAGGGAAAAAAACGAAAGGGAGGCAATGGAAGTTGGATTGGCAAATATTGCCATTAATTCCGATTCTAAACCGGTCAAAGATGTCCCATCTAGTAGTAAACCCTCGAGGGCAGCCATAAGTAGTTCATCCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000245 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016071 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016779 GO:0019219 GO:0019222 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0023052 GO:0031323 GO:0031325 GO:0032153 GO:0032386 GO:0032388 GO:0032507 GO:0032879 GO:0032880 GO:0033036 GO:0033120 GO:0033157 GO:0033365 GO:0034504 GO:0034613 GO:0034622 GO:0034641 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045185 GO:0045935 GO:0046483 GO:0046822 GO:0046824 GO:0046825 GO:0046827 GO:0048024 GO:0048026 GO:0048518 GO:0048522 GO:0050265 GO:0050684 GO:0050685 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051235 GO:0051252 GO:0051254 GO:0051457 GO:0051641 GO:0051651 GO:0051716 GO:0060255 GO:0060341 GO:0065003 GO:0065007 GO:0065008 GO:0070201 GO:0070569 GO:0070727 GO:0071704 GO:0071826 GO:0071840 GO:0072595 GO:0080090 GO:0090087 GO:0090304 GO:0090316 GO:0140096 GO:0140098 GO:1901360 GO:1901564 GO:1903311 GO:1903313 GO:1903827 GO:1903829 GO:1904951
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

552

Amino Acids

62.01

Weight (kDa)

6.59

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1624
Acc36I ACCTGC 1 cut(s) 1433
AccB1I GGYRCC 2 cut(s) 62, 1032
AccB7I CCANNNNNTGG 1 cut(s) 174
AccBSI CCGCTC 1 cut(s) 123
AccII CGCG 2 cut(s) 81, 728
AclWI GGATC 7 cut(s) 569, 582, 883, 1039, 1127, 1176, 1442
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 2 cut(s) 583, 1328
AcuI CTGAAG 2 cut(s) 971, 1530
AfaI GTAC 4 cut(s) 386, 419, 898, 992
AfiI CCNNNNNNNGG 6 cut(s) 174, 244, 571, 924, 1142, 1306
AflIII ACRYGT 1 cut(s) 45
AgeI ACCGGT 1 cut(s) 1591
AgsI TTSAA 5 cut(s) 118, 277, 529, 588, 799
AhdI GACNNNNNGTC 2 cut(s) 46, 131
AhlI ACTAGT 1 cut(s) 827
AjiI CACGTC 1 cut(s) 48
AjnI CCWGG 4 cut(s) 12, 182, 883, 1649
AjuI GAANNNNNNNTTGG 2 cut(s) 944, 976
AleI CACNNNNGTG 1 cut(s) 1143
AluBI AGCT 8 cut(s) 148, 325, 505, 716, 743, 1108, 1192, 1472
AluI AGCT 8 cut(s) 148, 325, 505, 716, 743, 1108, 1192, 1472
Alw21I GWGCWC 2 cut(s) 595, 1128
Alw26I GTCTC 2 cut(s) 288, 675
AlwI GGATC 7 cut(s) 569, 582, 883, 1039, 1127, 1176, 1442
AlwNI CAGNNNCTG 1 cut(s) 860
Ama87I CYCGRG 1 cut(s) 1624
AoxI GGCC 4 cut(s) 157, 252, 1295, 1400
ApeKI GCWGC 5 cut(s) 36, 361, 713, 1409, 1631
ApoI RAATTY 2 cut(s) 583, 1328
AseI ATTAAT 1 cut(s) 1575
AsiGI ACCGGT 1 cut(s) 1591
AspLEI GCGC 3 cut(s) 81, 728, 1290
AspS9I GGNCC 6 cut(s) 157, 331, 350, 866, 1296, 1445
AsuC2I CCSGG 2 cut(s) 299, 431
AsuHPI GGTGA 7 cut(s) 331, 404, 1054, 1130, 1157, 1274, 1511
AsuII TTCGAA 1 cut(s) 100
AvaI CYCGRG 1 cut(s) 1624
AvaII GGWCC 4 cut(s) 331, 350, 866, 1445
AxyI CCTNAGG 1 cut(s) 1222
BaeGI GKGCMC 1 cut(s) 1037
BamHI GGATCC 1 cut(s) 574
BanI GGYRCC 2 cut(s) 62, 1032
BarI GAAGNNNNNNTAC 2 cut(s) 880, 912
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 2 cut(s) 595, 1128
BbvI GCAGC 5 cut(s) 48, 373, 725, 1396, 1643
BccI CCATC 7 cut(s) 278, 573, 857, 1180, 1382, 1471, 1615
BceAI ACGGC 2 cut(s) 80, 1415
BcgI CGANNNNNNTGC 2 cut(s) 1325, 1359
BciT130I CCWGG 4 cut(s) 14, 184, 885, 1651
BcnI CCSGG 2 cut(s) 299, 431
BcoDI GTCTC 2 cut(s) 288, 675
BcuI ACTAGT 1 cut(s) 827
BfaI CTAG 3 cut(s) 201, 828, 1611
BfmI CTRYAG 1 cut(s) 1467
BfuAI ACCTGC 1 cut(s) 1433
BglII AGATCT 1 cut(s) 1075
BisI GCNGC 9 cut(s) 37, 82, 85, 252, 362, 714, 1282, 1410, 1632
BlsI GCNGC 9 cut(s) 38, 83, 86, 253, 363, 715, 1283, 1411, 1633
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 6 cut(s) 14, 184, 299, 431, 885, 1651
Bme18I GGWCC 4 cut(s) 331, 350, 866, 1445
BmeRI GACNNNNNGTC 2 cut(s) 46, 131
BmeT110I CYCGRG 1 cut(s) 1624
BmgBI CACGTC 1 cut(s) 48
BmgT120I GGNCC 6 cut(s) 157, 331, 350, 866, 1296, 1445
BmiI GGNNCC 8 cut(s) 64, 158, 351, 576, 677, 1034, 1298, 1461
BmrFI CCNGG 6 cut(s) 14, 184, 299, 431, 885, 1651
BmsI GCATC 6 cut(s) 736, 788, 847, 947, 1194, 1333
BpiI GAAGAC 1 cut(s) 75
Bpu14I TTCGAA 1 cut(s) 100
BpuEI CTTGAG 2 cut(s) 554, 702
BpuMI CCSGG 2 cut(s) 299, 431
BsaAI YACGTR 1 cut(s) 446
BsaBI GATNNNNATC 2 cut(s) 269, 1167
BsaI GGTCTC 1 cut(s) 288
BsaJI CCNNGG 6 cut(s) 183, 255, 430, 768, 1048, 1430
BsaWI WCCGGW 1 cut(s) 1591
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 6 cut(s) 174, 244, 571, 924, 1142, 1306
Bse118I RCCGGY 2 cut(s) 75, 1591
Bse1I ACTGG 1 cut(s) 1120
Bse21I CCTNAGG 1 cut(s) 1222
Bse3DI GCAATG 1 cut(s) 1551
Bse8I GATNNNNATC 2 cut(s) 269, 1167
BseBI CCWGG 4 cut(s) 14, 184, 885, 1651
BseDI CCNNGG 6 cut(s) 183, 255, 430, 768, 1048, 1430
BseGI GGATG 8 cut(s) 265, 307, 670, 761, 1168, 1374, 1426, 1646
BseJI GATNNNNATC 2 cut(s) 269, 1167
BseLI CCNNNNNNNGG 6 cut(s) 174, 244, 571, 924, 1142, 1306
BseMI GCAATG 1 cut(s) 1551
BseMII CTCAG 1 cut(s) 1383
BseNI ACTGG 1 cut(s) 1120
BseSI GKGCMC 1 cut(s) 1037
BseXI GCAGC 5 cut(s) 48, 373, 725, 1396, 1643
BsgI GTGCAG 3 cut(s) 732, 1395, 1461
Bsh1236I CGCG 2 cut(s) 81, 728
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 4 cut(s) 159, 254, 1297, 1402
BshNI GGYRCC 2 cut(s) 62, 1032
BshTI ACCGGT 1 cut(s) 1591
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 2 cut(s) 595, 1128
BsiHKCI CYCGRG 1 cut(s) 1624
BsiSI CCGG 5 cut(s) 76, 299, 430, 533, 1592
BslFI GGGAC 4 cut(s) 200, 363, 1373, 1589
BslI CCNNNNNNNGG 6 cut(s) 174, 244, 571, 924, 1142, 1306
BsmAI GTCTC 2 cut(s) 288, 675
BsmFI GGGAC 4 cut(s) 200, 363, 1373, 1589
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 4 cut(s) 159, 254, 1297, 1402
Bso31I GGTCTC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 1624
Bsp119I TTCGAA 1 cut(s) 100
Bsp1286I GDGCHC 3 cut(s) 595, 1037, 1128
Bsp143I GATC 9 cut(s) 22, 574, 848, 875, 1044, 1075, 1132, 1168, 1434
Bsp19I CCATGG 2 cut(s) 255, 1430
BspANI GGCC 4 cut(s) 159, 254, 1297, 1402
BspCNI CTCAG 1 cut(s) 1384
BspFNI CGCG 2 cut(s) 81, 728
BspLI GGNNCC 8 cut(s) 64, 158, 351, 576, 677, 1034, 1298, 1461
BspMI ACCTGC 1 cut(s) 1433
BspPI GGATC 7 cut(s) 569, 582, 883, 1039, 1127, 1176, 1442
BspQI GCTCTTC 1 cut(s) 810
BspT104I TTCGAA 1 cut(s) 100
BspT107I GGYRCC 2 cut(s) 62, 1032
BspTNI GGTCTC 1 cut(s) 288
BsrBI CCGCTC 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 1551
BsrFI RCCGGY 2 cut(s) 75, 1591
BsrI ACTGG 1 cut(s) 1120
BssAI RCCGGY 2 cut(s) 75, 1591
BssECI CCNNGG 6 cut(s) 183, 255, 430, 768, 1048, 1430
BssMI GATC 9 cut(s) 22, 574, 848, 875, 1044, 1075, 1132, 1168, 1434
BssT1I CCWWGG 3 cut(s) 255, 768, 1430
Bst2UI CCWGG 4 cut(s) 14, 184, 885, 1651
Bst4CI ACNGT 8 cut(s) 129, 172, 422, 999, 1026, 1142, 1416, 1499
Bst6I CTCTTC 1 cut(s) 810
BstAPI GCANNNNNTGC 2 cut(s) 710, 1568
BstBAI YACGTR 1 cut(s) 446
BstBI TTCGAA 1 cut(s) 100
BstC8I GCNNGC 5 cut(s) 180, 711, 1093, 1110, 1286
BstDEI CTNAG 4 cut(s) 900, 1174, 1222, 1392
BstDSI CCRYGG 2 cut(s) 255, 1430
BstENI CCTNNNNNAGG 1 cut(s) 1304
BstF5I GGATG 8 cut(s) 265, 307, 670, 761, 1168, 1374, 1426, 1646
BstFNI CGCG 2 cut(s) 81, 728
BstHHI GCGC 3 cut(s) 81, 728, 1290
BstKTI GATC 9 cut(s) 25, 577, 851, 878, 1047, 1078, 1135, 1171, 1437
BstMAI GTCTC 2 cut(s) 288, 675
BstMBI GATC 9 cut(s) 22, 574, 848, 875, 1044, 1075, 1132, 1168, 1434
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 5 cut(s) 502, 710, 1287, 1294, 1568
BstNI CCWGG 4 cut(s) 14, 184, 885, 1651
BstNSI RCATGY 3 cut(s) 368, 984, 1095
BstSCI CCNGG 6 cut(s) 12, 182, 297, 429, 883, 1649
BstSFI CTRYAG 1 cut(s) 1467
BstSLI GKGCMC 1 cut(s) 1037
BstSNI TACGTA 1 cut(s) 446
BstUI CGCG 2 cut(s) 81, 728
BstV1I GCAGC 5 cut(s) 48, 373, 725, 1396, 1643
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 3 cut(s) 574, 875, 1075
BstYI RGATCY 3 cut(s) 574, 875, 1075
Bsu36I CCTNAGG 1 cut(s) 1222
BsuRI GGCC 4 cut(s) 159, 254, 1297, 1402
BtgI CCRYGG 2 cut(s) 255, 1430
BtrI CACGTC 1 cut(s) 48
BtsCI GGATG 8 cut(s) 265, 307, 670, 761, 1168, 1374, 1426, 1646
BtsIMutI CAGTG 5 cut(s) 427, 519, 1147, 1421, 1444
BveI ACCTGC 1 cut(s) 1433
Cac8I GCNNGC 5 cut(s) 180, 711, 1093, 1110, 1286
CaiI CAGNNNCTG 1 cut(s) 860
CfoI GCGC 3 cut(s) 81, 728, 1290
Cfr10I RCCGGY 2 cut(s) 75, 1591
Cfr13I GGNCC 6 cut(s) 157, 331, 350, 866, 1296, 1445
CseI GACGC 1 cut(s) 415
Csp6I GTAC 4 cut(s) 385, 418, 897, 991
CspAI ACCGGT 1 cut(s) 1591
CviAII CATG 6 cut(s) 256, 365, 470, 981, 1092, 1431
CviQI GTAC 4 cut(s) 385, 418, 897, 991
DdeI CTNAG 4 cut(s) 900, 1174, 1222, 1392
DpnI GATC 9 cut(s) 24, 576, 850, 877, 1046, 1077, 1134, 1170, 1436
DpnII GATC 9 cut(s) 22, 574, 848, 875, 1044, 1075, 1132, 1168, 1434
DriI GACNNNNNGTC 2 cut(s) 46, 131
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 810
Eam1105I GACNNNNNGTC 2 cut(s) 46, 131
EarI CTCTTC 1 cut(s) 810
EciI GGCGGA 3 cut(s) 68, 754, 940
Eco105I TACGTA 1 cut(s) 446
Eco130I CCWWGG 3 cut(s) 255, 768, 1430
Eco31I GGTCTC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 1012
Eco47I GGWCC 4 cut(s) 331, 350, 866, 1445
Eco57I CTGAAG 2 cut(s) 971, 1530
Eco81I CCTNAGG 1 cut(s) 1222
Eco88I CYCGRG 1 cut(s) 1624
EcoNI CCTNNNNNAGG 1 cut(s) 1304
EcoO109I RGGNCCY 1 cut(s) 1445
EcoRI GAATTC 1 cut(s) 1328
EcoRII CCWGG 4 cut(s) 12, 182, 883, 1649
EcoRV GATATC 1 cut(s) 1012
EcoT14I CCWWGG 3 cut(s) 255, 768, 1430
EcoT22I ATGCAT 2 cut(s) 982, 1097
ErhI CCWWGG 3 cut(s) 255, 768, 1430
FaeI CATG 6 cut(s) 259, 368, 473, 984, 1095, 1434
FalI AAGNNNNNCTT 2 cut(s) 1205, 1237
FaqI GGGAC 4 cut(s) 200, 363, 1373, 1589
FatI CATG 6 cut(s) 255, 364, 469, 980, 1091, 1430
Fnu4HI GCNGC 9 cut(s) 37, 82, 85, 252, 362, 714, 1282, 1410, 1632
FokI GGATG 8 cut(s) 272, 314, 677, 748, 1175, 1361, 1413, 1633
Fsp4HI GCNGC 9 cut(s) 37, 82, 85, 252, 362, 714, 1282, 1410, 1632
FspBI CTAG 3 cut(s) 201, 828, 1611
GlaI GCGC 3 cut(s) 80, 727, 1289
GluI GCNGC 9 cut(s) 37, 82, 85, 252, 362, 714, 1282, 1410, 1632
HaeIII GGCC 4 cut(s) 159, 254, 1297, 1402
HapII CCGG 5 cut(s) 76, 299, 430, 533, 1592
HgaI GACGC 1 cut(s) 415
HhaI GCGC 3 cut(s) 81, 728, 1290
Hin1II CATG 6 cut(s) 259, 368, 473, 984, 1095, 1434
Hin6I GCGC 3 cut(s) 79, 726, 1288
HinP1I GCGC 3 cut(s) 79, 726, 1288
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 5 cut(s) 28, 102, 626, 635, 1583
HpaII CCGG 5 cut(s) 76, 299, 430, 533, 1592
HphI GGTGA 7 cut(s) 331, 404, 1054, 1130, 1157, 1274, 1511
Hpy166II GTNNAC 4 cut(s) 405, 1002, 1495, 1619
Hpy188III TCNNGA 7 cut(s) 118, 274, 329, 348, 588, 719, 1239
Hpy8I GTNNAC 4 cut(s) 405, 1002, 1495, 1619
Hpy99I CGWCG 2 cut(s) 23, 52
HpyAV CCTTC 2 cut(s) 53, 1430
HpyCH4III ACNGT 8 cut(s) 129, 172, 422, 999, 1026, 1142, 1416, 1499
HpyCH4IV ACGT 3 cut(s) 47, 445, 989
HpyF10VI GCNNNNNNNGC 5 cut(s) 502, 710, 1287, 1294, 1568
HpyF3I CTNAG 4 cut(s) 900, 1174, 1222, 1392
HpySE526I ACGT 3 cut(s) 47, 445, 989
Hsp92II CATG 6 cut(s) 259, 368, 473, 984, 1095, 1434
HspAI GCGC 3 cut(s) 79, 726, 1288
Kzo9I GATC 9 cut(s) 22, 574, 848, 875, 1044, 1075, 1132, 1168, 1434
LguI GCTCTTC 1 cut(s) 810
LmnI GCTCC 1 cut(s) 1189
Lsp1109I GCAGC 5 cut(s) 48, 373, 725, 1396, 1643
LweI GCATC 6 cut(s) 736, 788, 847, 947, 1194, 1333
MaeI CTAG 3 cut(s) 201, 828, 1611
MaeII ACGT 3 cut(s) 47, 445, 989
MaeIII GTNAC 3 cut(s) 1145, 1369, 1499
MalI GATC 9 cut(s) 24, 576, 850, 877, 1046, 1077, 1134, 1170, 1436
MbiI CCGCTC 1 cut(s) 123
MboI GATC 9 cut(s) 22, 574, 848, 875, 1044, 1075, 1132, 1168, 1434
MboII GAAGA 7 cut(s) 80, 596, 694, 708, 827, 1415, 1523
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 3 cut(s) 574, 875, 1075
MhlI GDGCHC 3 cut(s) 595, 1037, 1128
MluCI AATT 6 cut(s) 583, 942, 959, 1328, 1352, 1576
MlyI GAGTC 2 cut(s) 37, 644
MmeI TCCRAC 4 cut(s) 460, 602, 965, 1534
Mph1103I ATGCAT 2 cut(s) 982, 1097
MseI TTAA 7 cut(s) 339, 414, 453, 656, 786, 1251, 1575
MslI CAYNNNNRTG 1 cut(s) 1143
MspI CCGG 5 cut(s) 76, 299, 430, 533, 1592
MspR9I CCNGG 6 cut(s) 14, 184, 299, 431, 885, 1651
MunI CAATTG 1 cut(s) 942
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 4 cut(s) 14, 184, 885, 1651
MvnI CGCG 2 cut(s) 81, 728
MwoI GCNNNNNNNGC 5 cut(s) 502, 710, 1287, 1294, 1568
NciI CCSGG 2 cut(s) 299, 431
NcoI CCATGG 2 cut(s) 255, 1430
NdeII GATC 9 cut(s) 22, 574, 848, 875, 1044, 1075, 1132, 1168, 1434
NlaIII CATG 6 cut(s) 259, 368, 473, 984, 1095, 1434
NlaIV GGNNCC 8 cut(s) 64, 158, 351, 576, 677, 1034, 1298, 1461
NmeAIII GCCGAG 1 cut(s) 1029
NmuCI GTSAC 2 cut(s) 1145, 1499
NsiI ATGCAT 2 cut(s) 982, 1097
NspI RCATGY 3 cut(s) 368, 984, 1095
NspV TTCGAA 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 1143
PaeI GCATGC 1 cut(s) 1095
PaeR7I CTCGAG 1 cut(s) 1624
PciSI GCTCTTC 1 cut(s) 810
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 343
PfeI GAWTC 3 cut(s) 102, 626, 1583
PflFI GACNNNGTC 1 cut(s) 1385
PflMI CCANNNNNTGG 1 cut(s) 174
PfoI TCCNGGA 1 cut(s) 883
PinAI ACCGGT 1 cut(s) 1591
PkrI GCNGC 9 cut(s) 38, 83, 86, 253, 363, 715, 1283, 1411, 1633
Ple19I CGATCG 1 cut(s) 25
PleI GAGTC 2 cut(s) 36, 643
PpsI GAGTC 2 cut(s) 36, 643
Ppu21I YACGTR 1 cut(s) 446
PpuMI RGGWCCY 1 cut(s) 1445
PshBI ATTAAT 1 cut(s) 1575
Psp5II RGGWCCY 1 cut(s) 1445
Psp6I CCWGG 4 cut(s) 12, 182, 883, 1649
PspGI CCWGG 4 cut(s) 12, 182, 883, 1649
PspN4I GGNNCC 8 cut(s) 64, 158, 351, 576, 677, 1034, 1298, 1461
PspPI GGNCC 6 cut(s) 157, 331, 350, 866, 1296, 1445
PspPPI RGGWCCY 1 cut(s) 1445
PspXI VCTCGAGB 1 cut(s) 1624
PsrI GAACNNNNNNTAC 2 cut(s) 983, 1015
PstNI CAGNNNCTG 1 cut(s) 860
PsuI RGATCY 3 cut(s) 574, 875, 1075
PsyI GACNNNGTC 1 cut(s) 1385
PvuI CGATCG 1 cut(s) 25
RsaI GTAC 4 cut(s) 386, 419, 898, 992
RsaNI GTAC 4 cut(s) 385, 418, 897, 991
RseI CAYNNNNRTG 1 cut(s) 1143
SapI GCTCTTC 1 cut(s) 810
SaqAI TTAA 7 cut(s) 339, 414, 453, 656, 786, 1251, 1575
SatI GCNGC 9 cut(s) 37, 82, 85, 252, 362, 714, 1282, 1410, 1632
Sau3AI GATC 9 cut(s) 22, 574, 848, 875, 1044, 1075, 1132, 1168, 1434
Sau96I GGNCC 6 cut(s) 157, 331, 350, 866, 1296, 1445
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 2 cut(s) 37, 644
ScrFI CCNGG 6 cut(s) 14, 184, 299, 431, 885, 1651
SduI GDGCHC 3 cut(s) 595, 1037, 1128
SfaNI GCATC 6 cut(s) 736, 788, 847, 947, 1194, 1333
SfcI CTRYAG 1 cut(s) 1467
Sfr274I CTCGAG 1 cut(s) 1624
SfuI TTCGAA 1 cut(s) 100
SinI GGWCC 4 cut(s) 331, 350, 866, 1445
SlaI CTCGAG 1 cut(s) 1624
SmiMI CAYNNNNRTG 1 cut(s) 1143
SmlI CTYRAG 3 cut(s) 569, 717, 1624
SmoI CTYRAG 3 cut(s) 569, 717, 1624
SnaBI TACGTA 1 cut(s) 446
SpeI ACTAGT 1 cut(s) 827
SphI GCATGC 1 cut(s) 1095
Sse9I AATT 6 cut(s) 583, 942, 959, 1328, 1352, 1576
SspI AATATT 2 cut(s) 1061, 1567
SspMI CTAG 3 cut(s) 201, 828, 1611
StyD4I CCNGG 6 cut(s) 12, 182, 297, 429, 883, 1649
StyI CCWWGG 3 cut(s) 255, 768, 1430
TaaI ACNGT 8 cut(s) 129, 172, 422, 999, 1026, 1142, 1416, 1499
TaiI ACGT 3 cut(s) 50, 448, 992
TaqI TCGA 7 cut(s) 100, 381, 813, 1231, 1380, 1450, 1625
TasI AATT 6 cut(s) 583, 942, 959, 1328, 1352, 1576
TatI WGTACW 2 cut(s) 384, 417
TauI GCSGC 4 cut(s) 84, 87, 254, 1284
TfiI GAWTC 3 cut(s) 102, 626, 1583
Tru1I TTAA 7 cut(s) 339, 414, 453, 656, 786, 1251, 1575
Tru9I TTAA 7 cut(s) 339, 414, 453, 656, 786, 1251, 1575
TscAI CASTG 5 cut(s) 427, 526, 1147, 1421, 1444
TseFI GTSAC 2 cut(s) 1145, 1499
TseI GCWGC 5 cut(s) 36, 361, 713, 1409, 1631
Tsp45I GTSAC 2 cut(s) 1145, 1499
TspDTI ATGAA 5 cut(s) 618, 753, 1341, 1415, 1635
TspGWI ACGGA 1 cut(s) 260
TspRI CASTG 5 cut(s) 427, 526, 1147, 1421, 1444
Tth111I GACNNNGTC 1 cut(s) 1385
Van91I CCANNNNNTGG 1 cut(s) 174
VpaK11BI GGWCC 4 cut(s) 331, 350, 866, 1445
VspI ATTAAT 1 cut(s) 1575
XagI CCTNNNNNAGG 1 cut(s) 1304
XapI RAATTY 2 cut(s) 583, 1328
XceI RCATGY 3 cut(s) 368, 984, 1095
XhoI CTCGAG 1 cut(s) 1624
XspI CTAG 3 cut(s) 201, 828, 1611
ZrmI AGTACT 1 cut(s) 386
Zsp2I ATGCAT 2 cut(s) 982, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.