MD12G1102800.v1.1

dnaJ homolog subfamily C member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015042
Physical Location & Seq
Forward (+)
16084615 .. 16086428
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1102800.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGCGACCCGCCTTGGGCGACTAACGGTAGCTGCGTTGTCGTCTCAGATTCGGAGATCAACGGCCCACTATTCGTCTCTGACCGCGATCAGCGTCGGCCAGGGTGGGTCCCAAACGAGACTGTGGTCTTCATCATCGTCTAGTAAATCTGATAAGGCGGAGAAGAAGAAGCCGTTAGGAGACCGCCTATCGGCGGTGATAGACGCCGTTAACGACAGTAAACTTCCTCCTGAGCTCCGTGGTCAGCGCAACAACATCAGGTCAGAAACAGATATAATCAATGTGGTGGAGCAAAGAATATGGCATTCTATGGAAGCAGGGCAGTTTGAGAATTTGCCGGGGAAAGGGAAGCCCCTAAACTTAAGTTCAAATCCTCACGCAGACCCAGCTGAGGACACTTTGTACCGAATCCTCTCAAAGAATGGCTGCGCGCCACAGTGGGTTGAGCTCAACAAAGAGATAAGGAGCCAGGTTTCGGAATGGAGAATCGCTCTGAAGAAGGCTTGCGCAAGCAAAAATTGCAACGGGGGCGGGGACGGGGGTGATCAATCGAAATGGGTTGAAGCTTCAGAGGCTTTGAAATTGCAAGTGAAACAGATCAATAATAAGGTACTGCAGTATAATCTTATGGCTCCTTTCGGCCGTCAAATGTTTGGACTCAACTGGGACAAGGAGCTGGATCGTTTGAAATCGGAGGAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1992

Protein Analysis

235

Amino Acids

25.92

Weight (kDa)

9.44

Isoelectric Point (pI)

51.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DJC28_CD PF09350 95 - 160 1.7e-22 DnaJ homologue, subfamily C, member 28, conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015042)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03560
fragaria_vesca FvH4_6g17220
malus_domestica MD12G1102800.v1.1
prunus_persica Prupe.7G122900_v2.0.a1
pyrus_communis pycom12g10090
rosa_chinensis RchiOBHm_Chr3g0471301
rosa_laevigata RLG00000024189
rosa_multiflora Rmu_co8050216.1_g000001 Rmu_sc0020748.1_g000002
rosa_roxburghii Rroxscaffold_6G00410090
rosa_rugosa Rorug03G0116900
rosa_samantha Rh3AG166400 Rh3BG191800 Rh3CG182100 Rh3DG183900
rosa_wichuraiana Rw3G015660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 508
AccII CGCG 2 cut(s) 86, 431
AciI CCGC 6 cut(s) 10, 84, 158, 184, 194, 531
AclWI GGATC 1 cut(s) 687
AcoI YGGCCR 2 cut(s) 97, 640
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 2 cut(s) 515, 552
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 2 cut(s) 404, 612
AfiI CCNNNNNNNGG 6 cut(s) 15, 190, 193, 344, 391, 475
AflII CTTAAG 1 cut(s) 361
AgsI TTSAA 4 cut(s) 369, 563, 580, 688
AjnI CCWGG 2 cut(s) 99, 468
AluBI AGCT 6 cut(s) 32, 235, 389, 448, 566, 676
AluI AGCT 6 cut(s) 32, 235, 389, 448, 566, 676
Alw21I GWGCWC 2 cut(s) 237, 450
Alw26I GTCTC 4 cut(s) 48, 81, 112, 174
AlwI GGATC 1 cut(s) 687
AoxI GGCC 3 cut(s) 63, 97, 640
ApeKI GCWGC 2 cut(s) 32, 426
ApoI RAATTY 1 cut(s) 331
AspLEI GCGC 4 cut(s) 249, 431, 433, 509
AspS9I GGNCC 2 cut(s) 64, 108
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 2 cut(s) 208, 554
AvaII GGWCC 1 cut(s) 108
BaeI ACNNNNGTAYC 2 cut(s) 602, 635
BanII GRGCYC 2 cut(s) 237, 450
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 2 cut(s) 237, 450
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 2 cut(s) 19, 413
BceAI ACGGC 4 cut(s) 78, 157, 191, 627
BciT130I CCWGG 2 cut(s) 101, 470
BclI TGATCA 1 cut(s) 544
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 4 cut(s) 48, 81, 112, 174
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 614
BfrI CTTAAG 1 cut(s) 361
BisI GCNGC 2 cut(s) 33, 427
BlsI GCNGC 2 cut(s) 34, 428
Bme1390I CCNGG 3 cut(s) 101, 339, 470
Bme18I GGWCC 1 cut(s) 108
BmgT120I GGNCC 2 cut(s) 64, 108
BmiI GGNNCC 4 cut(s) 109, 110, 467, 633
BmrFI CCNGG 3 cut(s) 101, 339, 470
BmrI ACTGGG 1 cut(s) 673
BmuI ACTGGG 1 cut(s) 673
BoxI GACNNNNGTC 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 120
BplI GAGNNNNNCTC 2 cut(s) 475, 507
Bpu10I CCTNAGC 2 cut(s) 231, 390
BpuMI CCSGG 1 cut(s) 339
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 174
BsaJI CCNNGG 4 cut(s) 13, 100, 238, 338
Bsc4I CCNNNNNNNGG 6 cut(s) 15, 190, 193, 344, 391, 475
Bse1I ACTGG 1 cut(s) 668
BseBI CCWGG 2 cut(s) 101, 470
BseDI CCNNGG 4 cut(s) 13, 100, 238, 338
BseLI CCNNNNNNNGG 6 cut(s) 15, 190, 193, 344, 391, 475
BseMII CTCAG 3 cut(s) 59, 222, 381
BseNI ACTGG 1 cut(s) 668
BsePI GCGCGC 1 cut(s) 429
BseX3I CGGCCG 1 cut(s) 640
BseXI GCAGC 2 cut(s) 19, 413
BseYI CCCAGC 1 cut(s) 385
Bsh1236I CGCG 2 cut(s) 86, 431
Bsh1285I CGRYCG 1 cut(s) 643
BshFI GGCC 3 cut(s) 65, 99, 642
BsiEI CGRYCG 1 cut(s) 643
BsiHKAI GWGCWC 2 cut(s) 237, 450
BsiSI CCGG 1 cut(s) 338
BslFI GGGAC 3 cut(s) 94, 548, 680
BslI CCNNNNNNNGG 6 cut(s) 15, 190, 193, 344, 391, 475
BsmAI GTCTC 4 cut(s) 48, 81, 112, 174
BsmBI CGTCTC 2 cut(s) 48, 81
BsmFI GGGAC 3 cut(s) 94, 548, 680
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 3 cut(s) 65, 99, 642
Bso31I GGTCTC 1 cut(s) 174
Bsp1286I GDGCHC 2 cut(s) 237, 450
Bsp143I GATC 5 cut(s) 56, 87, 544, 597, 679
BspACI CCGC 6 cut(s) 10, 84, 158, 184, 194, 531
BspANI GGCC 3 cut(s) 65, 99, 642
BspCNI CTCAG 3 cut(s) 58, 223, 382
BspFNI CGCG 2 cut(s) 86, 431
BspLI GGNNCC 4 cut(s) 109, 110, 467, 633
BspMAI CTGCAG 1 cut(s) 618
BspPI GGATC 1 cut(s) 687
BspTI CTTAAG 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 174
BsrI ACTGG 1 cut(s) 668
BssECI CCNNGG 4 cut(s) 13, 100, 238, 338
BssHII GCGCGC 1 cut(s) 429
BssMI GATC 5 cut(s) 56, 87, 544, 597, 679
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 13
Bst2UI CCWGG 2 cut(s) 101, 470
Bst4CI ACNGT 4 cut(s) 28, 123, 218, 438
BstACI GRCGYC 1 cut(s) 204
BstAFI CTTAAG 1 cut(s) 361
BstAPI GCANNNNNTGC 1 cut(s) 519
BstC8I GCNNGC 3 cut(s) 431, 505, 511
BstDEI CTNAG 3 cut(s) 45, 231, 390
BstDSI CCRYGG 1 cut(s) 238
BstFNI CGCG 2 cut(s) 86, 431
BstHHI GCGC 4 cut(s) 249, 431, 433, 509
BstKTI GATC 5 cut(s) 59, 90, 547, 600, 682
BstMAI GTCTC 4 cut(s) 48, 81, 112, 174
BstMBI GATC 5 cut(s) 56, 87, 544, 597, 679
BstMCI CGRYCG 1 cut(s) 643
BstMWI GCNNNNNNNGC 4 cut(s) 386, 519, 528, 572
BstNI CCWGG 2 cut(s) 101, 470
BstPAI GACNNNNGTC 1 cut(s) 124
BstSCI CCNGG 3 cut(s) 99, 337, 468
BstSFI CTRYAG 1 cut(s) 614
BstUI CGCG 2 cut(s) 86, 431
BstV1I GCAGC 2 cut(s) 19, 413
BstV2I GAAGAC 1 cut(s) 120
BstZI CGGCCG 1 cut(s) 640
BsuRI GGCC 3 cut(s) 65, 99, 642
BtgI CCRYGG 1 cut(s) 238
BtsIMutI CAGTG 1 cut(s) 443
Cac8I GCNNGC 3 cut(s) 431, 505, 511
CfoI GCGC 4 cut(s) 249, 431, 433, 509
Cfr13I GGNCC 2 cut(s) 64, 108
CseI GACGC 2 cut(s) 82, 212
Csp6I GTAC 2 cut(s) 403, 611
CviQI GTAC 2 cut(s) 403, 611
DdeI CTNAG 3 cut(s) 45, 231, 390
DpnI GATC 5 cut(s) 58, 89, 546, 599, 681
DpnII GATC 5 cut(s) 56, 87, 544, 597, 679
EaeI YGGCCR 2 cut(s) 97, 640
EagI CGGCCG 1 cut(s) 640
EciI GGCGGA 1 cut(s) 173
Ecl136II GAGCTC 2 cut(s) 235, 448
EclXI CGGCCG 1 cut(s) 640
Eco130I CCWWGG 1 cut(s) 13
Eco24I GRGCYC 2 cut(s) 237, 450
Eco31I GGTCTC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 108
Eco52I CGGCCG 1 cut(s) 640
Eco53kI GAGCTC 2 cut(s) 235, 448
Eco57I CTGAAG 2 cut(s) 515, 552
EcoICRI GAGCTC 2 cut(s) 235, 448
EcoO109I RGGNCCY 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 99, 468
EcoT14I CCWWGG 1 cut(s) 13
EcoT38I GRGCYC 2 cut(s) 237, 450
ErhI CCWWGG 1 cut(s) 13
Esp3I CGTCTC 2 cut(s) 48, 81
FaiI YATR 5 cut(s) 275, 301, 311, 621, 629
FaqI GGGAC 3 cut(s) 94, 548, 680
FauI CCCGC 2 cut(s) 17, 524
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 2 cut(s) 33, 427
FriOI GRGCYC 2 cut(s) 237, 450
Fsp4HI GCNGC 2 cut(s) 33, 427
FspBI CTAG 1 cut(s) 141
FspI TGCGCA 1 cut(s) 508
GlaI GCGC 4 cut(s) 248, 430, 432, 508
GluI GCNGC 2 cut(s) 33, 427
GsaI CCCAGC 1 cut(s) 389
HaeIII GGCC 3 cut(s) 65, 99, 642
HapII CCGG 1 cut(s) 338
HgaI GACGC 2 cut(s) 82, 212
HhaI GCGC 4 cut(s) 249, 431, 433, 509
Hin1I GRCGYC 1 cut(s) 204
Hin6I GCGC 4 cut(s) 247, 429, 431, 507
HinP1I GCGC 4 cut(s) 247, 429, 431, 507
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 564
HinfI GANTC 4 cut(s) 49, 408, 486, 657
HpaI GTTAAC 1 cut(s) 211
HpaII CCGG 1 cut(s) 338
HphI GGTGA 2 cut(s) 208, 554
Hpy166II GTNNAC 2 cut(s) 211, 221
Hpy188I TCNGA 9 cut(s) 48, 54, 81, 151, 265, 478, 495, 571, 694
Hpy188III TCNNGA 1 cut(s) 230
Hpy8I GTNNAC 2 cut(s) 211, 221
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 1 cut(s) 493
HpyCH4III ACNGT 4 cut(s) 28, 123, 218, 438
HpyCH4V TGCA 3 cut(s) 522, 586, 616
HpyF10VI GCNNNNNNNGC 4 cut(s) 386, 519, 528, 572
HpyF3I CTNAG 3 cut(s) 45, 231, 390
Hsp92I GRCGYC 1 cut(s) 204
HspAI GCGC 4 cut(s) 247, 429, 431, 507
KflI GGGWCCC 1 cut(s) 108
Ksp22I TGATCA 1 cut(s) 544
KspAI GTTAAC 1 cut(s) 211
Kzo9I GATC 5 cut(s) 56, 87, 544, 597, 679
LmnI GCTCC 5 cut(s) 240, 289, 465, 637, 673
Lsp1109I GCAGC 2 cut(s) 19, 413
MaeI CTAG 1 cut(s) 141
MalI GATC 5 cut(s) 58, 89, 546, 599, 681
MboI GATC 5 cut(s) 56, 87, 544, 597, 679
MboII GAAGA 4 cut(s) 120, 175, 178, 508
MhlI GDGCHC 2 cut(s) 237, 450
MluCI AATT 3 cut(s) 331, 517, 581
MlyI GAGTC 1 cut(s) 651
MnlI CCTC 6 cut(s) 237, 384, 385, 422, 565, 688
MseI TTAA 2 cut(s) 210, 362
MspA1I CMGCKG 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 361
MspI CCGG 1 cut(s) 338
MspR9I CCNGG 3 cut(s) 101, 339, 470
Mva1269I GAATGC 1 cut(s) 304
MvaI CCWGG 2 cut(s) 101, 470
MvnI CGCG 2 cut(s) 86, 431
MwoI GCNNNNNNNGC 4 cut(s) 386, 519, 528, 572
NciI CCSGG 1 cut(s) 339
NdeII GATC 5 cut(s) 56, 87, 544, 597, 679
NlaIV GGNNCC 4 cut(s) 109, 110, 467, 633
NsbI TGCGCA 1 cut(s) 508
PauI GCGCGC 1 cut(s) 429
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 210
PctI GAATGC 1 cut(s) 304
PfeI GAWTC 3 cut(s) 49, 408, 486
PkrI GCNGC 2 cut(s) 34, 428
PleI GAGTC 1 cut(s) 651
PpsI GAGTC 1 cut(s) 651
PpuMI RGGWCCY 1 cut(s) 108
PshAI GACNNNNGTC 1 cut(s) 124
Psp124BI GAGCTC 2 cut(s) 237, 450
Psp5II RGGWCCY 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 99, 468
PspFI CCCAGC 1 cut(s) 385
PspGI CCWGG 2 cut(s) 99, 468
PspN4I GGNNCC 4 cut(s) 109, 110, 467, 633
PspPI GGNCC 2 cut(s) 64, 108
PspPPI RGGWCCY 1 cut(s) 108
PstI CTGCAG 1 cut(s) 618
PteI GCGCGC 1 cut(s) 429
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 2 cut(s) 404, 612
RsaNI GTAC 2 cut(s) 403, 611
SacI GAGCTC 2 cut(s) 237, 450
SaqAI TTAA 2 cut(s) 210, 362
SatI GCNGC 2 cut(s) 33, 427
Sau3AI GATC 5 cut(s) 56, 87, 544, 597, 679
Sau96I GGNCC 2 cut(s) 64, 108
SchI GAGTC 1 cut(s) 651
ScrFI CCNGG 3 cut(s) 101, 339, 470
SduI GDGCHC 2 cut(s) 237, 450
SetI ASST 9 cut(s) 34, 237, 263, 391, 450, 474, 568, 612, 678
SfcI CTRYAG 1 cut(s) 614
SinI GGWCC 1 cut(s) 108
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
Sse9I AATT 3 cut(s) 331, 517, 581
SsiI CCGC 6 cut(s) 10, 84, 158, 184, 194, 531
SspMI CTAG 1 cut(s) 141
SstI GAGCTC 2 cut(s) 237, 450
StyD4I CCNGG 3 cut(s) 99, 337, 468
StyI CCWWGG 1 cut(s) 13
TaaI ACNGT 4 cut(s) 28, 123, 218, 438
TaqI TCGA 1 cut(s) 551
TasI AATT 3 cut(s) 331, 517, 581
TfiI GAWTC 3 cut(s) 49, 408, 486
Tru1I TTAA 2 cut(s) 210, 362
Tru9I TTAA 2 cut(s) 210, 362
TscAI CASTG 1 cut(s) 443
TseI GCWGC 2 cut(s) 32, 426
TspDTI ATGAA 1 cut(s) 120
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 1 cut(s) 443
Vha464I CTTAAG 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 108
XapI RAATTY 1 cut(s) 331
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.