Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005478

Physical Location & Seq

Genomic Coordinates Forward (+)

21495356 .. 21500680

Gene Structure 5325 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1137500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1485 bp

ATGAGGACCAACGGCGGGAAGATATCCAAGCCTTCGTGGCCGAAAGTGATGGCGAGGAAATGGCTCAACATACCAATCAAGGGGGATGAGTTTCATTCCGATTACTCTCCAAAATATGAGACTGGGAGAAGGAAGAGCTGCTCGGACCAAGACCGCTACGTCGTCGTACCGGACGATTTTTCAGATAGATGCACGTTGATGGGGGAAACAGCCCATAGACAAAGTTGTGGAATGGAGCCGCCTACTGTCACTGATGATCCCAATCTCAAGATGTTCGTAGGGACATGGAATGTTGGAGGCAAATCACCCCAGGACAACTTGAACTTGAGGGAATGGCTGAAATCTCCTACTCCCGCAGACGTCTATGTTCTTGGGTTCCAGGAAATTGTCCCTTTGAACGCCGGGAACGTATTGGGCGCGGAAGATAAGGGCCCAGCTGCCAAGTGGCTGTCCCTGATTCGCGAGGCACTGAACAAGAATGACCCTGGACAGCTTGACCAGCAAGGCAAGCCTAGGCTCAGCTTCTCCGACTTGCTTTCTTTAGAAGATGAGCTTAGCAGTGCAGATTTTGAGAGACTTCTGAATTTGAATCCCGCGTCGACTTCAAGTGGAGAAAACTCACCAACCTCGCCAGTGATGTGTCAGTGGAAAGGCAATAGTCCAATGCAAGGAAATCGTTACTGCCTATCAGCAAGCAAGCAGATGGTCGGAATTTTCATGTGCGTGTGGGTTCGTGCTGATCTTTGTAGACACATTAGCAACACGAAGGTCTCGTGTGTTGGTAGAGGCATAATGGGATACCTTGGAAACAAGGGTTCAATATCAATCAGCATGACGTTGCAAGGAACAACATTTTGTTTCGTGTGTGCACATTTAACCTCTGGGGAGAAAGAAGGGGATGAGATGAGAAGGAACTCAGATGTCATGGAAATCTTAAAGAAAACAAGATTTTCTCATTCATGTAGAACAAAGGGACAACTGCTTCCTCCCAATAGCATTTTAGACCATGACAAGGTTATTTGGCTCGGGGATTTAAATTATCGGCTAGCTGCTGCTTGCTGCGGCGACACACATGAACTATTAAAGAAGCATGATTGGCAGGCACTTCTAGAGAAAGATCAGCTAAGGATAGAGCAACAAGCAGGTCGAGTTTTTAAAGGGTGGGAGGAAGGGAGGATATATTTTGCTCCAACCTACAAATACCTAGCTAATTCTGATCATTATGTTGCCCAAAGTTCTAAATCTAGAGATAAGAGACGCACTCCAGCCTGGTGTGACAGGATTTTATGGAAGGGGGAAGGGCTTAAACAAATGTCGTATGTGAGAGGGGAGTCGAAATTCTCAGACCACAGACCGGTTTCTTCGTTGTTTTCAGTCTGGTTAGACTTGGCTGACAAGAAAAAGCTCAACTCTTGCATGCTCAAGTCATCAGCCAAAGTGCAGGCGGAAGAGCTTTTGCTGCTCACGGAACCAAAAGCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004439 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0030258 GO:0034485 GO:0034593 GO:0034594 GO:0034595 GO:0042578 GO:0044237 GO:0044238 GO:0044255 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0052866 GO:0071704 GO:0106019

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04070 map00562 map01100 map04070

Pfam Domains

Protein Families

Exo_endo_phos

Protein Analysis

495

Amino Acids

55.52

Weight (kDa)

8.81

Isoelectric Point (pI)

40.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exo_endo_phos2	PF22669	94 - 162	1e-07	Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos2	PF22669	223 - 455	8.9e-61	Endonuclease/Exonuclease/phosphatase family 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005478)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37440 AT2G37440 AT2G37440
fragaria_vesca	FvH4_6g14390 FvH4_6g14390 FvH4_6g14390 FvH4_6g14390 FvH4_6g14390 FvH4_6g14390
malus_domestica	MD04G1120100.v1.1 MD12G1137500.v1.1
prunus_persica	Prupe.6G249400_v2.0.a1 Prupe.6G249400_v2.0.a1 Prupe.6G249400_v2.0.a1 Prupe.6G249400_v2.0.a1
pyrus_communis	pycom04g10930 pycom12g13150
rosa_chinensis	RchiOBHm_Chr3g0467461
rosa_laevigata	RLG00000024503
rosa_multiflora	Rmu_sc0001601.1_g000003
rosa_roxburghii	Rroxscaffold_6G00413660
rosa_rugosa	Rorug03G0087200 Rorug03G0087300 Rorug03G0087400
rosa_samantha	Rh3AG135600 Rh3BG157200 Rh3CG157100 Rh3DG158000
rosa_wichuraiana	Rw3G012880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	158
AatII	GACGTC	1 cut(s)	363
Acc36I	ACCTGC	1 cut(s)	1133
AccI	GTMKAC	2 cut(s)	599, 748
AccII	CGCG	3 cut(s)	419, 462, 596
AciI	CCGC	8 cut(s)	15, 154, 239, 354, 419, 594, 1062, 1445
AclWI	GGATC	1 cut(s)	251
AcoI	YGGCCR	1 cut(s)	38
AcsI	RAATTY	3 cut(s)	583, 711, 1337
AcyI	GRCGYC	1 cut(s)	360
AfaI	GTAC	1 cut(s)	168
AfiI	CCNNNNNNNGG	5 cut(s)	15, 80, 668, 1012, 1354
AgeI	ACCGGT	1 cut(s)	1354
AgsI	TTSAA	5 cut(s)	322, 397, 589, 606, 819
AjnI	CCWGG	4 cut(s)	309, 378, 484, 1268
Alw21I	GWGCWC	1 cut(s)	871
Alw26I	GTCTC	4 cut(s)	113, 568, 775, 1249
Alw44I	GTGCAC	1 cut(s)	867
AlwI	GGATC	1 cut(s)	251
Ama87I	CYCGRG	1 cut(s)	1025
AoxI	GGCC	2 cut(s)	38, 430
ApaI	GGGCCC	1 cut(s)	434
ApaLI	GTGCAC	1 cut(s)	867
ApeKI	GCWGC	7 cut(s)	138, 437, 1049, 1052, 1059, 1459, 1478
ApoI	RAATTY	3 cut(s)	583, 711, 1337
AsiGI	ACCGGT	1 cut(s)	1354
AspA2I	CCTAGG	1 cut(s)	512
AspLEI	GCGC	1 cut(s)	419
AspS9I	GGNCC	4 cut(s)	6, 145, 430, 431
AsuC2I	CCSGG	1 cut(s)	403
AsuHPI	GGTGA	2 cut(s)	297, 612
AsuNHI	GCTAGC	1 cut(s)	1045
AvaI	CYCGRG	1 cut(s)	1025
AvaII	GGWCC	2 cut(s)	6, 145
AvrII	CCTAGG	1 cut(s)	512
BaeGI	GKGCMC	2 cut(s)	434, 871
BanII	GRGCYC	1 cut(s)	434
BauI	CACGAG	1 cut(s)	772
Bbv12I	GWGCWC	1 cut(s)	871
BbvI	GCAGC	7 cut(s)	125, 424, 1036, 1039, 1046, 1446, 1465
BccI	CCATC	3 cut(s)	43, 193, 697
BceAI	ACGGC	1 cut(s)	28
BcgI	CGANNNNNNTGC	2 cut(s)	656, 690
BciT130I	CCWGG	4 cut(s)	311, 380, 486, 1270
BciVI	GTATCC	1 cut(s)	791
BclI	TGATCA	1 cut(s)	1216
BcnI	CCSGG	1 cut(s)	403
BcoDI	GTCTC	4 cut(s)	113, 568, 775, 1249
BfaI	CTAG	5 cut(s)	513, 1046, 1109, 1205, 1245
BfuAI	ACCTGC	1 cut(s)	1133
BfuI	GTATCC	1 cut(s)	791
BglI	GCCNNNNNGGC	1 cut(s)	37
BisI	GCNGC	9 cut(s)	139, 239, 438, 1050, 1053, 1060, 1063, 1460, 1479
BlnI	CCTAGG	1 cut(s)	512
BlpI	GCTNAGC	2 cut(s)	518, 554
BlsI	GCNGC	9 cut(s)	140, 240, 439, 1051, 1054, 1061, 1064, 1461, 1480
Bme1390I	CCNGG	5 cut(s)	311, 380, 403, 486, 1270
Bme18I	GGWCC	2 cut(s)	6, 145
BmeT110I	CYCGRG	1 cut(s)	1025
BmgT120I	GGNCC	4 cut(s)	6, 145, 430, 431
BmiI	GGNNCC	4 cut(s)	237, 377, 432, 1470
BmrFI	CCNGG	5 cut(s)	311, 380, 403, 486, 1270
BmrI	ACTGGG	1 cut(s)	132
BmsI	GCATC	1 cut(s)	179
BmtI	GCTAGC	1 cut(s)	1049
BmuI	ACTGGG	1 cut(s)	132
BplI	GAGNNNNNCTC	2 cut(s)	1246, 1278
BpmI	CTGGAG	1 cut(s)	1248
Bpu10I	CCTNAGC	1 cut(s)	1124
Bpu1102I	GCTNAGC	2 cut(s)	518, 554
BpuEI	CTTGAG	3 cut(s)	251, 346, 1406
BpuMI	CCSGG	1 cut(s)	403
BsaBI	GATNNNNATC	1 cut(s)	261
BsaHI	GRCGYC	1 cut(s)	360
BsaI	GGTCTC	1 cut(s)	775
BsaJI	CCNNGG	4 cut(s)	309, 484, 512, 802
BsaWI	WCCGGW	2 cut(s)	169, 1354
Bsc4I	CCNNNNNNNGG	5 cut(s)	15, 80, 668, 1012, 1354
Bse118I	RCCGGY	1 cut(s)	1354
Bse1I	ACTGG	2 cut(s)	127, 632
Bse8I	GATNNNNATC	1 cut(s)	261
BseBI	CCWGG	4 cut(s)	311, 380, 486, 1270
BseDI	CCNNGG	4 cut(s)	309, 484, 512, 802
BseGI	GGATG	2 cut(s)	91, 904
BseJI	GATNNNNATC	1 cut(s)	261
BseLI	CCNNNNNNNGG	5 cut(s)	15, 80, 668, 1012, 1354
BseMII	CTCAG	3 cut(s)	532, 930, 1356
BseNI	ACTGG	2 cut(s)	127, 632
BseSI	GKGCMC	2 cut(s)	434, 871
BseXI	GCAGC	7 cut(s)	125, 424, 1036, 1039, 1046, 1446, 1465
BseYI	CCCAGC	1 cut(s)	433
BsgI	GTGCAG	2 cut(s)	582, 1460
Bsh1236I	CGCG	3 cut(s)	419, 462, 596
BshFI	GGCC	2 cut(s)	40, 432
BshTI	ACCGGT	1 cut(s)	1354
BsiHKAI	GWGCWC	1 cut(s)	871
BsiHKCI	CYCGRG	1 cut(s)	1025
BsiSI	CCGG	3 cut(s)	170, 402, 1355
BslFI	GGGAC	4 cut(s)	295, 374, 436, 987
BslI	CCNNNNNNNGG	5 cut(s)	15, 80, 668, 1012, 1354
BsmAI	GTCTC	4 cut(s)	113, 568, 775, 1249
BsmBI	CGTCTC	1 cut(s)	1249
BsmFI	GGGAC	4 cut(s)	295, 374, 436, 987
BsnI	GGCC	2 cut(s)	40, 432
Bso31I	GGTCTC	1 cut(s)	775
BsoBI	CYCGRG	1 cut(s)	1025
Bsp120I	GGGCCC	1 cut(s)	430
Bsp1286I	GDGCHC	2 cut(s)	434, 871
Bsp143I	GATC	4 cut(s)	256, 739, 1117, 1216
Bsp1720I	GCTNAGC	2 cut(s)	518, 554
Bsp68I	TCGCGA	1 cut(s)	462
BspACI	CCGC	8 cut(s)	15, 154, 239, 354, 419, 594, 1062, 1445
BspANI	GGCC	2 cut(s)	40, 432
BspCNI	CTCAG	3 cut(s)	531, 929, 1355
BspFNI	CGCG	3 cut(s)	419, 462, 596
BspLI	GGNNCC	4 cut(s)	237, 377, 432, 1470
BspMI	ACCTGC	1 cut(s)	1133
BspOI	GCTAGC	1 cut(s)	1049
BspPI	GGATC	1 cut(s)	251
BspQI	GCTCTTC	2 cut(s)	128, 1443
BspTNI	GGTCTC	1 cut(s)	775
BsrFI	RCCGGY	1 cut(s)	1354
BsrI	ACTGG	2 cut(s)	127, 632
BssAI	RCCGGY	1 cut(s)	1354
BssECI	CCNNGG	4 cut(s)	309, 484, 512, 802
BssMI	GATC	4 cut(s)	256, 739, 1117, 1216
BssNI	GRCGYC	1 cut(s)	360
BssSI	CACGAG	1 cut(s)	772
BssT1I	CCWWGG	2 cut(s)	512, 802
Bst2BI	CACGAG	1 cut(s)	772
Bst2UI	CCWGG	4 cut(s)	311, 380, 486, 1270
Bst4CI	ACNGT	1 cut(s)	247
Bst6I	CTCTTC	2 cut(s)	128, 1443
BstACI	GRCGYC	1 cut(s)	360
BstC8I	GCNNGC	8 cut(s)	509, 694, 698, 1047, 1057, 1101, 1418, 1443
BstDEI	CTNAG	6 cut(s)	518, 554, 916, 1124, 1342, 1482
BstF5I	GGATG	2 cut(s)	91, 904
BstFNI	CGCG	3 cut(s)	419, 462, 596
BstHHI	GCGC	1 cut(s)	419
BstKTI	GATC	4 cut(s)	259, 742, 1120, 1219
BstMAI	GTCTC	4 cut(s)	113, 568, 775, 1249
BstMBI	GATC	4 cut(s)	256, 739, 1117, 1216
BstMWI	GCNNNNNNNGC	5 cut(s)	37, 499, 508, 1096, 1459
BstNI	CCWGG	4 cut(s)	311, 380, 486, 1270
BstNSI	RCATGY	1 cut(s)	1420
BstSCI	CCNGG	5 cut(s)	309, 378, 401, 484, 1268
BstSLI	GKGCMC	2 cut(s)	434, 871
BstUI	CGCG	3 cut(s)	419, 462, 596
BstV1I	GCAGC	7 cut(s)	125, 424, 1036, 1039, 1046, 1446, 1465
BsuI	GTATCC	1 cut(s)	791
BsuRI	GGCC	2 cut(s)	40, 432
BtsCI	GGATG	2 cut(s)	91, 904
BtsI	GCAGTG	1 cut(s)	565
BtsIMutI	CAGTG	5 cut(s)	249, 467, 565, 639, 650
BtuMI	TCGCGA	1 cut(s)	462
BveI	ACCTGC	1 cut(s)	1133
Cac8I	GCNNGC	8 cut(s)	509, 694, 698, 1047, 1057, 1101, 1418, 1443
CfoI	GCGC	1 cut(s)	419
Cfr10I	RCCGGY	1 cut(s)	1354
Cfr13I	GGNCC	4 cut(s)	6, 145, 430, 431
CseI	GACGC	2 cut(s)	585, 1266
Csp6I	GTAC	1 cut(s)	167
CspAI	ACCGGT	1 cut(s)	1354
CviAII	CATG	9 cut(s)	285, 718, 832, 925, 960, 1007, 1073, 1091, 1417
CviQI	GTAC	1 cut(s)	167
DdeI	CTNAG	6 cut(s)	518, 554, 916, 1124, 1342, 1482
DpnI	GATC	4 cut(s)	258, 741, 1119, 1218
DpnII	GATC	4 cut(s)	256, 739, 1117, 1216
DraI	TTTAAA	2 cut(s)	1035, 1156
DrdI	GACNNNNNNGTC	1 cut(s)	158
DseDI	GACNNNNNNGTC	1 cut(s)	158
EaeI	YGGCCR	1 cut(s)	38
Eam1104I	CTCTTC	2 cut(s)	128, 1443
EarI	CTCTTC	2 cut(s)	128, 1443
EciI	GGCGGA	1 cut(s)	1460
Eco130I	CCWWGG	2 cut(s)	512, 802
Eco24I	GRGCYC	1 cut(s)	434
Eco31I	GGTCTC	1 cut(s)	775
Eco32I	GATATC	1 cut(s)	24
Eco47I	GGWCC	2 cut(s)	6, 145
Eco88I	CYCGRG	1 cut(s)	1025
EcoO109I	RGGNCCY	1 cut(s)	430
EcoRII	CCWGG	4 cut(s)	309, 378, 484, 1268
EcoRV	GATATC	1 cut(s)	24
EcoT14I	CCWWGG	2 cut(s)	512, 802
EcoT38I	GRGCYC	1 cut(s)	434
ErhI	CCWWGG	2 cut(s)	512, 802
Esp3I	CGTCTC	1 cut(s)	1249
FaeI	CATG	9 cut(s)	288, 721, 835, 928, 963, 1010, 1076, 1094, 1420
FalI	AAGNNNNNCTT	2 cut(s)	537, 569
FaqI	GGGAC	4 cut(s)	295, 374, 436, 987
FatI	CATG	9 cut(s)	284, 717, 831, 924, 959, 1006, 1072, 1090, 1416
FauI	CCCGC	3 cut(s)	8, 361, 601
FbaI	TGATCA	1 cut(s)	1216
FblI	GTMKAC	2 cut(s)	599, 748
Fnu4HI	GCNGC	9 cut(s)	139, 239, 438, 1050, 1053, 1060, 1063, 1460, 1479
FokI	GGATG	2 cut(s)	98, 911
FriOI	GRGCYC	1 cut(s)	434
Fsp4HI	GCNGC	9 cut(s)	139, 239, 438, 1050, 1053, 1060, 1063, 1460, 1479
FspBI	CTAG	5 cut(s)	513, 1046, 1109, 1205, 1245
GlaI	GCGC	1 cut(s)	418
GluI	GCNGC	9 cut(s)	139, 239, 438, 1050, 1053, 1060, 1063, 1460, 1479
GsaI	CCCAGC	1 cut(s)	437
GsuI	CTGGAG	1 cut(s)	1248
HaeIII	GGCC	2 cut(s)	40, 432
HapII	CCGG	3 cut(s)	170, 402, 1355
HgaI	GACGC	2 cut(s)	585, 1266
HhaI	GCGC	1 cut(s)	419
Hin1I	GRCGYC	1 cut(s)	360
Hin1II	CATG	9 cut(s)	288, 721, 835, 928, 963, 1010, 1076, 1094, 1420
Hin6I	GCGC	1 cut(s)	417
HinP1I	GCGC	1 cut(s)	417
HincII	GTYRAC	1 cut(s)	600
HindII	GTYRAC	1 cut(s)	600
HinfI	GANTC	3 cut(s)	457, 589, 1331
HpaII	CCGG	3 cut(s)	170, 402, 1355
HphI	GGTGA	2 cut(s)	297, 612
Hpy166II	GTNNAC	3 cut(s)	600, 749, 869
Hpy188I	TCNGA	9 cut(s)	100, 145, 184, 529, 582, 710, 919, 1216, 1345
Hpy188III	TCNNGA	4 cut(s)	268, 461, 1109, 1245
Hpy8I	GTNNAC	3 cut(s)	600, 749, 869
Hpy99I	CGWCG	3 cut(s)	164, 167, 601
HpyAV	CCTTC	8 cut(s)	42, 123, 760, 887, 903, 1163, 1285, 1292
HpyCH4III	ACNGT	1 cut(s)	247
HpyCH4IV	ACGT	5 cut(s)	159, 194, 360, 408, 836
HpyCH4V	TGCA	7 cut(s)	192, 563, 667, 841, 869, 1416, 1441
HpyF10VI	GCNNNNNNNGC	5 cut(s)	37, 499, 508, 1096, 1459
HpyF3I	CTNAG	6 cut(s)	518, 554, 916, 1124, 1342, 1482
HpySE526I	ACGT	5 cut(s)	159, 194, 360, 408, 836
Hsp92I	GRCGYC	1 cut(s)	360
Hsp92II	CATG	9 cut(s)	288, 721, 835, 928, 963, 1010, 1076, 1094, 1420
HspAI	GCGC	1 cut(s)	417
Ksp22I	TGATCA	1 cut(s)	1216
Kzo9I	GATC	4 cut(s)	256, 739, 1117, 1216
LguI	GCTCTTC	2 cut(s)	128, 1443
LmnI	GCTCC	2 cut(s)	235, 1192
Lsp1109I	GCAGC	7 cut(s)	125, 424, 1036, 1039, 1046, 1446, 1465
LweI	GCATC	1 cut(s)	179
MaeI	CTAG	5 cut(s)	513, 1046, 1109, 1205, 1245
MaeII	ACGT	5 cut(s)	159, 194, 360, 408, 836
MaeIII	GTNAC	3 cut(s)	247, 677, 1274
MalI	GATC	4 cut(s)	258, 741, 1119, 1218
MboI	GATC	4 cut(s)	256, 739, 1117, 1216
MboII	GAAGA	6 cut(s)	31, 145, 434, 557, 1353, 1460
MhlI	GDGCHC	2 cut(s)	434, 871
MluCI	AATT	6 cut(s)	384, 583, 711, 1036, 1210, 1337
MlyI	GAGTC	1 cut(s)	1340
MmeI	TCCRAC	4 cut(s)	274, 552, 688, 1214
MseI	TTAA	6 cut(s)	875, 935, 1034, 1082, 1155, 1305
MslI	CAYNNNNRTG	2 cut(s)	197, 722
MspA1I	CMGCKG	1 cut(s)	437
MspI	CCGG	3 cut(s)	170, 402, 1355
MspR9I	CCNGG	5 cut(s)	311, 380, 403, 486, 1270
MvaI	CCWGG	4 cut(s)	311, 380, 486, 1270
MvnI	CGCG	3 cut(s)	419, 462, 596
MwoI	GCNNNNNNNGC	5 cut(s)	37, 499, 508, 1096, 1459
NciI	CCSGG	1 cut(s)	403
NdeII	GATC	4 cut(s)	256, 739, 1117, 1216
NheI	GCTAGC	1 cut(s)	1045
NlaIII	CATG	9 cut(s)	288, 721, 835, 928, 963, 1010, 1076, 1094, 1420
NlaIV	GGNNCC	4 cut(s)	237, 377, 432, 1470
NmuCI	GTSAC	2 cut(s)	247, 1274
NruI	TCGCGA	1 cut(s)	462
NspI	RCATGY	1 cut(s)	1420
PaeI	GCATGC	1 cut(s)	1420
PciSI	GCTCTTC	2 cut(s)	128, 1443
PcsI	WCGNNNNNNNCGW	3 cut(s)	171, 405, 770
PfeI	GAWTC	2 cut(s)	457, 589
PfoI	TCCNGGA	1 cut(s)	378
PinAI	ACCGGT	1 cut(s)	1354
PkrI	GCNGC	9 cut(s)	140, 240, 439, 1051, 1054, 1061, 1064, 1461, 1480
PleI	GAGTC	1 cut(s)	1339
PpsI	GAGTC	1 cut(s)	1339
Psp6I	CCWGG	4 cut(s)	309, 378, 484, 1268
PspFI	CCCAGC	1 cut(s)	433
PspGI	CCWGG	4 cut(s)	309, 378, 484, 1268
PspN4I	GGNNCC	4 cut(s)	237, 377, 432, 1470
PspOMI	GGGCCC	1 cut(s)	430
PspPI	GGNCC	4 cut(s)	6, 145, 430, 431
PvuII	CAGCTG	1 cut(s)	437
RruI	TCGCGA	1 cut(s)	462
RsaI	GTAC	1 cut(s)	168
RsaNI	GTAC	1 cut(s)	167
RseI	CAYNNNNRTG	2 cut(s)	197, 722
SalI	GTCGAC	1 cut(s)	598
SapI	GCTCTTC	2 cut(s)	128, 1443
SaqAI	TTAA	6 cut(s)	875, 935, 1034, 1082, 1155, 1305
SatI	GCNGC	9 cut(s)	139, 239, 438, 1050, 1053, 1060, 1063, 1460, 1479
Sau3AI	GATC	4 cut(s)	256, 739, 1117, 1216
Sau96I	GGNCC	4 cut(s)	6, 145, 430, 431
SchI	GAGTC	1 cut(s)	1340
ScrFI	CCNGG	5 cut(s)	311, 380, 403, 486, 1270
SduI	GDGCHC	2 cut(s)	434, 871
SfaNI	GCATC	1 cut(s)	179
SinI	GGWCC	2 cut(s)	6, 145
SmiI	ATTTAAAT	1 cut(s)	1035
SmiMI	CAYNNNNRTG	2 cut(s)	197, 722
SmlI	CTYRAG	3 cut(s)	266, 325, 1421
SmoI	CTYRAG	3 cut(s)	266, 325, 1421
SphI	GCATGC	1 cut(s)	1420
Sse9I	AATT	6 cut(s)	384, 583, 711, 1036, 1210, 1337
SsiI	CCGC	8 cut(s)	15, 154, 239, 354, 419, 594, 1062, 1445
SspMI	CTAG	5 cut(s)	513, 1046, 1109, 1205, 1245
StyD4I	CCNGG	5 cut(s)	309, 378, 401, 484, 1268
StyI	CCWWGG	2 cut(s)	512, 802
SwaI	ATTTAAAT	1 cut(s)	1035
TaaI	ACNGT	1 cut(s)	247
TaiI	ACGT	5 cut(s)	162, 197, 363, 411, 839
TaqI	TCGA	3 cut(s)	599, 1147, 1334
TasI	AATT	6 cut(s)	384, 583, 711, 1036, 1210, 1337
TauI	GCSGC	2 cut(s)	241, 1065
TfiI	GAWTC	2 cut(s)	457, 589
Tru1I	TTAA	6 cut(s)	875, 935, 1034, 1082, 1155, 1305
Tru9I	TTAA	6 cut(s)	875, 935, 1034, 1082, 1155, 1305
TscAI	CASTG	5 cut(s)	256, 474, 565, 639, 650
TseFI	GTSAC	2 cut(s)	247, 1274
TseI	GCWGC	7 cut(s)	138, 437, 1049, 1052, 1059, 1459, 1478
Tsp45I	GTSAC	2 cut(s)	247, 1274
TspDTI	ATGAA	4 cut(s)	83, 706, 948, 1089
TspGWI	ACGGA	1 cut(s)	1481
TspRI	CASTG	5 cut(s)	256, 474, 565, 639, 650
VneI	GTGCAC	1 cut(s)	867
VpaK11BI	GGWCC	2 cut(s)	6, 145
XapI	RAATTY	3 cut(s)	583, 711, 1337
XbaI	TCTAGA	2 cut(s)	1108, 1244
XceI	RCATGY	1 cut(s)	1420
XcmI	CCANNNNNNNNNTGG	1 cut(s)	441
XmaJI	CCTAGG	1 cut(s)	512
XmiI	GTMKAC	2 cut(s)	599, 748
XspI	CTAG	5 cut(s)	513, 1046, 1109, 1205, 1245
ZraI	GACGTC	1 cut(s)	361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.