MD12G1154500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016667
Physical Location & Seq
Forward (+)
23496463 .. 23496915
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1154500.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGCTCAATATCAATTTCTTCTCCTTTTCTTGATCTTGGGAGCTGCGAGTGTAACCATGGCTAATGAAGTGGCTCATCAAACCCTAGAGCAGTCTACTTTAGCTTCACTCTCAAGCAATCCAGCTCCAAGTCCCTTTCCACCTATAACTAGGAAGTTGGGAAAGCACAACCCTAGTAAGACCAACCCTAAGTCTTCTGATGCGCCAGCTTTAAGCCCAAGAAGTGCACCATCTCCGACGAGTGAAACACCGGAGACCGGAGAGAGCGTGAGCATTCTTGAGCAGGAGATCCACATCCAAAAGCACCACCACTCAATGGACAAGTCGGTTGCCGGAGGCGGTGTGATCCTCGGAGGGCTTGCGACAACCTTCTTAGTGGCAATTTTCTGTTACATTAGAGCCACTGCAAAACATGAAGGTTCGGTGACTAGTACTACTGGTTCTAGTCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

15.72

Weight (kDa)

7.06

Isoelectric Point (pI)

59.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016667)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09280
fragaria_vesca FvH4_6g12750
malus_domestica MD12G1154500.v1.1
prunus_persica Prupe.6G264300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0464671
rosa_laevigata RLG00000024676
rosa_multiflora Rmu_sc0002031.1_g000003 Rmu_sc0030142.1_g000002
rosa_roxburghii Rroxscaffold_6G00415470
rosa_rugosa Rorug03G0073700
rosa_samantha Rh3BG136000 Rh3CG138600 Rh3DG137600
rosa_wichuraiana Rw3G011140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 316
AccI GTMKAC 1 cut(s) 95
AciI CCGC 1 cut(s) 339
AclWI GGATC 2 cut(s) 283, 340
AfaI GTAC 1 cut(s) 433
AfiI CCNNNNNNNGG 2 cut(s) 257, 316
AhlI ACTAGT 1 cut(s) 428
AluBI AGCT 4 cut(s) 44, 104, 125, 209
AluI AGCT 4 cut(s) 44, 104, 125, 209
Alw21I GWGCWC 1 cut(s) 229
Alw26I GTCTC 1 cut(s) 248
Alw44I GTGCAC 1 cut(s) 225
AlwI GGATC 2 cut(s) 283, 340
ApaLI GTGCAC 1 cut(s) 225
ApeKI GCWGC 1 cut(s) 44
AspLEI GCGC 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 436
BaeGI GKGCMC 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 229
BbvI GCAGC 1 cut(s) 31
BccI CCATC 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 248
BcuI ACTAGT 1 cut(s) 428
BfaI CTAG 6 cut(s) 86, 150, 174, 429, 444, 451
BisI GCNGC 1 cut(s) 45
BlsI GCNGC 1 cut(s) 46
BmcAI AGTACT 1 cut(s) 433
BmsI GCATC 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 186
BpuEI CTTGAG 2 cut(s) 97, 299
BsaBI GATNNNNATC 1 cut(s) 293
BsaI GGTCTC 1 cut(s) 248
BsaJI CCNNGG 2 cut(s) 57, 349
BsaWI WCCGGW 2 cut(s) 250, 257
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 327, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 316
Bse1I ACTGG 1 cut(s) 442
Bse8I GATNNNNATC 1 cut(s) 293
BseDI CCNNGG 2 cut(s) 57, 349
BseGI GGATG 1 cut(s) 294
BseJI GATNNNNATC 1 cut(s) 293
BseLI CCNNNNNNNGG 2 cut(s) 257, 316
BseNI ACTGG 1 cut(s) 442
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 229
BsiSI CCGG 3 cut(s) 251, 258, 333
BslFI GGGAC 2 cut(s) 117, 432
BslI CCNNNNNNNGG 2 cut(s) 257, 316
BsmAI GTCTC 1 cut(s) 248
BsmFI GGGAC 2 cut(s) 117, 432
BsmI GAATGC 1 cut(s) 273
Bso31I GGTCTC 1 cut(s) 248
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 3 cut(s) 33, 288, 345
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 1 cut(s) 339
BspPI GGATC 2 cut(s) 283, 340
BspTNI GGTCTC 1 cut(s) 248
BsrI ACTGG 1 cut(s) 442
BssECI CCNNGG 2 cut(s) 57, 349
BssMI GATC 3 cut(s) 33, 288, 345
BssT1I CCWWGG 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 207, 360
BstDEI CTNAG 2 cut(s) 189, 373
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 1 cut(s) 294
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 3 cut(s) 36, 291, 348
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 3 cut(s) 33, 288, 345
BstSLI GKGCMC 1 cut(s) 229
BstV1I GCAGC 1 cut(s) 31
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 288
BstYI RGATCY 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 1 cut(s) 294
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 2 cut(s) 207, 360
CfoI GCGC 1 cut(s) 205
Csp6I GTAC 1 cut(s) 432
CviAII CATG 2 cut(s) 58, 413
CviQI GTAC 1 cut(s) 432
DdeI CTNAG 2 cut(s) 189, 373
DpnI GATC 3 cut(s) 35, 290, 347
DpnII GATC 3 cut(s) 33, 288, 345
Eco130I CCWWGG 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 2 cut(s) 61, 416
FaiI YATR 3 cut(s) 59, 146, 414
FaqI GGGAC 2 cut(s) 117, 432
FatI CATG 2 cut(s) 57, 412
FblI GTMKAC 1 cut(s) 95
Fnu4HI GCNGC 1 cut(s) 45
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 1 cut(s) 45
FspBI CTAG 6 cut(s) 86, 150, 174, 429, 444, 451
GlaI GCGC 1 cut(s) 204
GluI GCNGC 1 cut(s) 45
HapII CCGG 3 cut(s) 251, 258, 333
HhaI GCGC 1 cut(s) 205
Hin1II CATG 2 cut(s) 61, 416
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HpaII CCGG 3 cut(s) 251, 258, 333
HphI GGTGA 1 cut(s) 436
Hpy166II GTNNAC 2 cut(s) 96, 227
Hpy188I TCNGA 3 cut(s) 199, 237, 353
Hpy188III TCNNGA 2 cut(s) 31, 278
Hpy8I GTNNAC 2 cut(s) 96, 227
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 2 cut(s) 379, 410
HpyCH4V TGCA 2 cut(s) 227, 407
HpyF3I CTNAG 2 cut(s) 189, 373
Hsp92II CATG 2 cut(s) 61, 416
HspAI GCGC 1 cut(s) 203
Kzo9I GATC 3 cut(s) 33, 288, 345
LmnI GCTCC 2 cut(s) 41, 130
LpnPI CCDG 7 cut(s) 135, 219, 264, 269, 271, 346, 423
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 1 cut(s) 190
MaeI CTAG 6 cut(s) 86, 150, 174, 429, 444, 451
MaeIII GTNAC 3 cut(s) 52, 389, 424
MalI GATC 3 cut(s) 35, 290, 347
MboI GATC 3 cut(s) 33, 288, 345
MboII GAAGA 2 cut(s) 11, 186
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 2 cut(s) 14, 381
MmeI TCCRAC 1 cut(s) 260
MnlI CCTC 3 cut(s) 329, 347, 359
MseI TTAA 1 cut(s) 212
MspI CCGG 3 cut(s) 251, 258, 333
Mva1269I GAATGC 1 cut(s) 273
NcoI CCATGG 1 cut(s) 57
NdeII GATC 3 cut(s) 33, 288, 345
NlaIII CATG 2 cut(s) 61, 416
NmuCI GTSAC 1 cut(s) 424
PctI GAATGC 1 cut(s) 273
PflMI CCANNNNNTGG 1 cut(s) 316
PkrI GCNGC 1 cut(s) 46
PsrI GAACNNNNNNTAC 1 cut(s) 424
PsuI RGATCY 1 cut(s) 288
RsaI GTAC 1 cut(s) 433
RsaNI GTAC 1 cut(s) 432
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 1 cut(s) 45
Sau3AI GATC 3 cut(s) 33, 288, 345
ScaI AGTACT 1 cut(s) 433
SduI GDGCHC 1 cut(s) 229
SetI ASST 7 cut(s) 46, 106, 127, 145, 211, 371, 421
SfaNI GCATC 1 cut(s) 190
SmlI CTYRAG 2 cut(s) 112, 278
SmoI CTYRAG 2 cut(s) 112, 278
SpeI ACTAGT 1 cut(s) 428
Sse9I AATT 2 cut(s) 14, 381
SsiI CCGC 1 cut(s) 339
SspMI CTAG 6 cut(s) 86, 150, 174, 429, 444, 451
StyI CCWWGG 1 cut(s) 57
TasI AATT 2 cut(s) 14, 381
TatI WGTACW 1 cut(s) 431
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 81, 429
TspRI CASTG 1 cut(s) 409
Van91I CCANNNNNTGG 1 cut(s) 316
VneI GTGCAC 1 cut(s) 225
XmiI GTMKAC 1 cut(s) 95
XspI CTAG 6 cut(s) 86, 150, 174, 429, 444, 451
ZrmI AGTACT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.