MD12G1166000.v1.1

LIMR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011383
Physical Location & Seq
Reverse (-)
24681510 .. 24685886
4377 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1166000.v1.1.491

Sequence Viewer

Length: 1527 bp
ATGGGCGATTTCAACCTCGCGCTCGTCATCGTCGCTATTATCCTCTGCATCATCGTCTTCGTCTTCAATGTCTACCTGCTCGTCAATTACCAGCACCCCGACGACAAGAACCAGGCCTACTTCCCCAAATTCGTCGTCGTTTTGGGGCTCTCCGTCGCCGCTATTTCAATACTCATGCTGCCGGCCGACGTGGCCAACCGCCAGGCGTGTCGCCACGCGATTTATAATGGCGCGTGCAATCTCACTCTTCCGATGAAGGATCTGTGGCTCGCAATCTATATCCTTGACGCCGTGCTCGTGTTCTTCATCATCCCCTTCGCTATGTTCTACTACGAAGGAGACCAGGACAAGAGTGTTGGAAAACGGATTGGAAGCGCGTTGTTGTGGGTTGCGGTGACCGCCGTTGTCTGCGCTCTCGTGCTCGGAATCTTATACGGGTTAGTTGGGAAGGTGGATTTTACTGTTAGGCACCTCTCTTCTTCCACAACTTCCTTTCCCAGTTCATTTCAATTTTCTAGCAGTAATGAATGCATAAATGGCGCAAAGGCGTGCTCTGCATATCTTGCAAGCGATACGTCAGAGAAAACATGGACAATGCGTACTACCTTTCCAGAATATGTCGTTGCACTTGCTACCATTGTTGGATCCGTGCTTTTTACTATATTCGGTGGTGTTGGCATTGCCTGTCTACCGCTGGGACTCATATTTTCATTCATTCGGCGTCCAAAGGCTGTTATTACTCGGTCACAGTATATCAAGGAAGCTACTGAACTGGGCAAGAAAGCAAAAGAGTTGAAGAAAGCAGCTGATGCGCTTCATCAAGAAGAAAGAGCTGGCTCTAAGGGTAGAAAATGGCGTAAAAATGTAAAGGCAGTAGAGAAGGAGTTGCTTCAATTAGAAGAAGATGTAAAACTTTTAGAAGAGGTGTATCCTCAAGGAGAGAAAGCTGAGACTACTTGGGCTATGACAGTTCTTGGGTACTTGGCAAAACTTGTGTTGGGAATCTTAGGATTGATTGTTTCAGTGGCTTGGATTGCTCATATTGTCATATATCTATTGATTAGCCCTCCGCTTTCTCCTTTCTTGAACGAAGTTTTCATCAAGCTGGATGATGTTTGGGGTCTTTTAGGCACTGTTGCGTTTGCATTCTTCTGCTTCTACCTTCTGCTTGCAGTCATTGCTGGGTCGATGATGCTTGGGCTGAAATTAGTTTTCATCACAATTCATCCGATGAAGTGGGGTGCTACTCTAATGAATTCATTTTTATTTAATGTGGGACTTATACTACTTAGCTCCATCAGTGTGATTCAGTTCTGCTCCACGGCATTTGCATACTATGCTCAAGCAACTGCAGCACAAGAAATATTTGGTCACACCTTGGAATCTCTTCGTGGAATTAGATATCTGTACAAGTACAATGTGTTCCAAATTGCATTTATTGTCCTGGCAGGACTGACGTTTGTGTACTATGCTGCTTTTGGCTGGAGAAGAAAAAAACCTAGCGGCAGGTTCCAACTTTCCTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04977 map04977
Pfam Domains
Protein Families
LMBR1

Protein Analysis

509

Amino Acids

56.31

Weight (kDa)

9.06

Isoelectric Point (pI)

31.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LMBR1 PF04791 9 - 275 4e-45 LMBR1-like membrane protein
LMBR1 PF04791 266 - 486 7.6e-27 LMBR1-like membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G08930 AT5G01460
fragaria_vesca FvH4_6g11450 FvH4_6g11450 FvH4_6g11450
malus_domestica MD04G1152700.v1.1 MD12G1166000.v1.1
prunus_persica Prupe.6G277300_v2.0.a1
pyrus_communis pycom04g13840 pycom12g15830
rosa_chinensis RchiOBHm_Chr3g0463141
rosa_laevigata RLG00000024810
rosa_multiflora Rmu_sc0008124.1_g000007
rosa_roxburghii Rroxscaffold_6G00416940
rosa_rugosa Rorug03G0061700
rosa_samantha Rh3AG119900 Rh3BG124300 Rh3CG126300 Rh3DG125300
rosa_wichuraiana Rw3G010140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 225
Acc36I ACCTGC 2 cut(s) 84, 1497
AccB1I GGYRCC 1 cut(s) 468
AccI GTMKAC 2 cut(s) 72, 688
AccII CGCG 4 cut(s) 20, 218, 233, 377
AciI CCGC 7 cut(s) 159, 199, 392, 399, 692, 1070, 1503
AclWI GGATC 3 cut(s) 267, 639, 652
AcoI YGGCCR 2 cut(s) 183, 192
AcsI RAATTY 2 cut(s) 128, 1255
AcyI GRCGYC 2 cut(s) 288, 721
AfaI GTAC 5 cut(s) 601, 980, 1409, 1415, 1466
AgsI TTSAA 7 cut(s) 13, 67, 168, 509, 796, 893, 1087
AjiI CACGTC 1 cut(s) 190
AjnI CCWGG 4 cut(s) 111, 201, 342, 1443
AluBI AGCT 6 cut(s) 764, 806, 833, 947, 1105, 1293
AluI AGCT 6 cut(s) 764, 806, 833, 947, 1105, 1293
Alw21I GWGCWC 3 cut(s) 297, 423, 554
Alw26I GTCTC 2 cut(s) 333, 944
AlwI GGATC 3 cut(s) 267, 639, 652
AoxI GGCC 3 cut(s) 114, 183, 192
ApeKI GCWGC 4 cut(s) 178, 803, 1352, 1472
ApoI RAATTY 2 cut(s) 128, 1255
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
Asp700I GAANNNNTTC 1 cut(s) 1386
AspLEI GCGC 6 cut(s) 22, 233, 377, 413, 542, 814
AsuHPI GGTGA 1 cut(s) 406
BalI TGGCCA 1 cut(s) 194
BamHI GGATCC 1 cut(s) 644
BanI GGYRCC 1 cut(s) 468
BanII GRGCYC 1 cut(s) 150
BauI CACGAG 2 cut(s) 296, 416
BbsI GAAGAC 2 cut(s) 49, 55
Bbv12I GWGCWC 3 cut(s) 297, 423, 554
BbvI GCAGC 4 cut(s) 165, 815, 1364, 1459
BccI CCATC 1 cut(s) 1304
BceAI ACGGC 3 cut(s) 275, 386, 1338
BciT130I CCWGG 4 cut(s) 113, 203, 344, 1445
BciVI GTATCC 1 cut(s) 939
BcoDI GTCTC 2 cut(s) 333, 944
BfaI CTAG 2 cut(s) 516, 1500
BfmI CTRYAG 1 cut(s) 1350
BfuAI ACCTGC 2 cut(s) 84, 1497
BfuI GTATCC 1 cut(s) 939
BglI GCCNNNNNGGC 1 cut(s) 191
BisI GCNGC 6 cut(s) 159, 179, 804, 1353, 1473, 1504
BlsI GCNGC 6 cut(s) 160, 180, 805, 1354, 1474, 1505
Bme1390I CCNGG 4 cut(s) 113, 203, 344, 1445
BmgBI CACGTC 1 cut(s) 190
BmiI GGNNCC 3 cut(s) 470, 646, 1511
BmrFI CCNGG 4 cut(s) 113, 203, 344, 1445
BmrI ACTGGG 2 cut(s) 492, 782
BmsI GCATC 3 cut(s) 57, 799, 1182
BmuI ACTGGG 2 cut(s) 492, 782
BpiI GAAGAC 2 cut(s) 49, 55
BpmI CTGGAG 1 cut(s) 1504
BpuEI CTTGAG 2 cut(s) 918, 1326
BsaHI GRCGYC 2 cut(s) 288, 721
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 1320, 1377
Bse118I RCCGGY 1 cut(s) 181
Bse1I ACTGG 2 cut(s) 498, 777
Bse3DI GCAATG 2 cut(s) 678, 1176
BseBI CCWGG 4 cut(s) 113, 203, 344, 1445
BseDI CCNNGG 2 cut(s) 1320, 1377
BseGI GGATG 3 cut(s) 309, 1114, 1225
BseMI GCAATG 2 cut(s) 678, 1176
BseMII CTCAG 1 cut(s) 939
BseNI ACTGG 2 cut(s) 498, 777
BseX3I CGGCCG 1 cut(s) 183
BseXI GCAGC 4 cut(s) 165, 815, 1364, 1459
BseYI CCCAGC 2 cut(s) 694, 1181
Bsh1236I CGCG 4 cut(s) 20, 218, 233, 377
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 3 cut(s) 116, 185, 194
BshNI GGYRCC 1 cut(s) 468
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 3 cut(s) 297, 423, 554
BsiSI CCGG 1 cut(s) 182
BslFI GGGAC 2 cut(s) 711, 1290
BsmAI GTCTC 2 cut(s) 333, 944
BsmFI GGGAC 2 cut(s) 711, 1290
BsmI GAATGC 2 cut(s) 533, 1145
BsnI GGCC 3 cut(s) 116, 185, 194
Bso31I GGTCTC 1 cut(s) 333
Bsp1286I GDGCHC 4 cut(s) 150, 297, 423, 554
Bsp1407I TGTACA 1 cut(s) 1407
Bsp143I GATC 2 cut(s) 259, 644
BspACI CCGC 7 cut(s) 159, 199, 392, 399, 692, 1070, 1503
BspANI GGCC 3 cut(s) 116, 185, 194
BspCNI CTCAG 1 cut(s) 940
BspFNI CGCG 4 cut(s) 20, 218, 233, 377
BspLI GGNNCC 3 cut(s) 470, 646, 1511
BspMAI CTGCAG 1 cut(s) 1354
BspMI ACCTGC 2 cut(s) 84, 1497
BspPI GGATC 3 cut(s) 267, 639, 652
BspT107I GGYRCC 1 cut(s) 468
BspTNI GGTCTC 1 cut(s) 333
BsrDI GCAATG 2 cut(s) 678, 1176
BsrFI RCCGGY 1 cut(s) 181
BsrGI TGTACA 1 cut(s) 1407
BsrI ACTGG 2 cut(s) 498, 777
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 2 cut(s) 1320, 1377
BssMI GATC 2 cut(s) 259, 644
BssNI GRCGYC 2 cut(s) 288, 721
BssSI CACGAG 2 cut(s) 296, 416
BssT1I CCWWGG 1 cut(s) 1377
Bst2BI CACGAG 2 cut(s) 296, 416
Bst2UI CCWGG 4 cut(s) 113, 203, 344, 1445
Bst4CI ACNGT 4 cut(s) 463, 750, 970, 1135
Bst6I CTCTTC 4 cut(s) 252, 481, 915, 1392
BstACI GRCGYC 2 cut(s) 288, 721
BstAPI GCANNNNNTGC 4 cut(s) 563, 809, 1178, 1337
BstAUI TGTACA 1 cut(s) 1407
BstC8I GCNNGC 7 cut(s) 183, 235, 270, 550, 568, 835, 1170
BstDEI CTNAG 4 cut(s) 840, 948, 1006, 1289
BstDSI CCRYGG 1 cut(s) 1320
BstEII GGTNACC 1 cut(s) 394
BstF5I GGATG 3 cut(s) 309, 1114, 1225
BstFNI CGCG 4 cut(s) 20, 218, 233, 377
BstHHI GCGC 6 cut(s) 22, 233, 377, 413, 542, 814
BstKTI GATC 2 cut(s) 262, 647
BstMAI GTCTC 2 cut(s) 333, 944
BstMBI GATC 2 cut(s) 259, 644
BstMCI CGRYCG 1 cut(s) 186
BstNI CCWGG 4 cut(s) 113, 203, 344, 1445
BstPI GGTNACC 1 cut(s) 394
BstSCI CCNGG 4 cut(s) 111, 201, 342, 1443
BstSFI CTRYAG 1 cut(s) 1350
BstUI CGCG 4 cut(s) 20, 218, 233, 377
BstV1I GCAGC 4 cut(s) 165, 815, 1364, 1459
BstV2I GAAGAC 2 cut(s) 49, 55
BstX2I RGATCY 2 cut(s) 259, 644
BstYI RGATCY 2 cut(s) 259, 644
BstZI CGGCCG 1 cut(s) 183
BsuI GTATCC 1 cut(s) 939
BsuRI GGCC 3 cut(s) 116, 185, 194
BtgI CCRYGG 1 cut(s) 1320
BtrI CACGTC 1 cut(s) 190
BtsCI GGATG 3 cut(s) 309, 1114, 1225
BtsIMutI CAGTG 3 cut(s) 1029, 1131, 1306
BveI ACCTGC 2 cut(s) 84, 1497
Cac8I GCNNGC 7 cut(s) 183, 235, 270, 550, 568, 835, 1170
CfoI GCGC 6 cut(s) 22, 233, 377, 413, 542, 814
Cfr10I RCCGGY 1 cut(s) 181
CseI GACGC 2 cut(s) 296, 710
Csp6I GTAC 5 cut(s) 600, 979, 1408, 1414, 1465
CspCI CAANNNNNGTGG 2 cut(s) 1309, 1344
CviAII CATG 2 cut(s) 175, 588
CviQI GTAC 5 cut(s) 600, 979, 1408, 1414, 1465
DdeI CTNAG 4 cut(s) 840, 948, 1006, 1289
DpnI GATC 2 cut(s) 261, 646
DpnII GATC 2 cut(s) 259, 644
EaeI YGGCCR 2 cut(s) 183, 192
EagI CGGCCG 1 cut(s) 183
Eam1104I CTCTTC 4 cut(s) 252, 481, 915, 1392
EarI CTCTTC 4 cut(s) 252, 481, 915, 1392
EclXI CGGCCG 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 1377
Eco147I AGGCCT 1 cut(s) 116
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 333
Eco32I GATATC 1 cut(s) 1403
Eco52I CGGCCG 1 cut(s) 183
Eco91I GGTNACC 1 cut(s) 394
EcoO65I GGTNACC 1 cut(s) 394
EcoRI GAATTC 1 cut(s) 1255
EcoRII CCWGG 4 cut(s) 111, 201, 342, 1443
EcoRV GATATC 1 cut(s) 1403
EcoT14I CCWWGG 1 cut(s) 1377
EcoT22I ATGCAT 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 1377
FaeI CATG 2 cut(s) 178, 591
FaqI GGGAC 2 cut(s) 711, 1290
FatI CATG 2 cut(s) 174, 587
FblI GTMKAC 2 cut(s) 72, 688
Fnu4HI GCNGC 6 cut(s) 159, 179, 804, 1353, 1473, 1504
FokI GGATG 3 cut(s) 296, 1121, 1212
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 6 cut(s) 159, 179, 804, 1353, 1473, 1504
FspBI CTAG 2 cut(s) 516, 1500
GlaI GCGC 6 cut(s) 21, 232, 376, 412, 541, 813
GluI GCNGC 6 cut(s) 159, 179, 804, 1353, 1473, 1504
GsaI CCCAGC 2 cut(s) 698, 1185
GsuI CTGGAG 1 cut(s) 1504
HaeIII GGCC 3 cut(s) 116, 185, 194
HapII CCGG 1 cut(s) 182
HgaI GACGC 2 cut(s) 296, 710
HhaI GCGC 6 cut(s) 22, 233, 377, 413, 542, 814
Hin1I GRCGYC 2 cut(s) 288, 721
Hin1II CATG 2 cut(s) 178, 591
Hin6I GCGC 6 cut(s) 20, 231, 375, 411, 540, 812
HinP1I GCGC 6 cut(s) 20, 231, 375, 411, 540, 812
HinfI GANTC 5 cut(s) 426, 699, 1002, 1306, 1382
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 3 cut(s) 73, 689, 1465
Hpy188I TCNGA 4 cut(s) 252, 425, 580, 1230
Hpy188III TCNNGA 3 cut(s) 611, 821, 1084
Hpy8I GTNNAC 3 cut(s) 73, 689, 1465
Hpy99I CGWCG 6 cut(s) 35, 104, 137, 140, 158, 191
HpyAV CCTTC 6 cut(s) 250, 325, 329, 442, 874, 1172
HpyCH4III ACNGT 4 cut(s) 463, 750, 970, 1135
HpyCH4IV ACGT 3 cut(s) 189, 575, 1457
HpyF3I CTNAG 4 cut(s) 840, 948, 1006, 1289
HpySE526I ACGT 3 cut(s) 189, 575, 1457
Hsp92I GRCGYC 2 cut(s) 288, 721
Hsp92II CATG 2 cut(s) 178, 591
HspAI GCGC 6 cut(s) 20, 231, 375, 411, 540, 812
KroI GCCGGC 1 cut(s) 181
KroNI GCCGGC 1 cut(s) 183
Kzo9I GATC 2 cut(s) 259, 644
LmnI GCTCC 2 cut(s) 1298, 1322
Lsp1109I GCAGC 4 cut(s) 165, 815, 1364, 1459
LweI GCATC 3 cut(s) 57, 799, 1182
MaeI CTAG 2 cut(s) 516, 1500
MaeII ACGT 3 cut(s) 189, 575, 1457
MaeIII GTNAC 3 cut(s) 394, 744, 1370
MalI GATC 2 cut(s) 261, 646
MboI GATC 2 cut(s) 259, 644
MflI RGATCY 2 cut(s) 259, 644
MhlI GDGCHC 4 cut(s) 150, 297, 423, 554
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 9 cut(s) 85, 128, 509, 893, 1205, 1221, 1255, 1395, 1428
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 1 cut(s) 693
MmeI TCCRAC 2 cut(s) 337, 622
MnlI CCTC 6 cut(s) 26, 53, 482, 916, 942, 1077
Mox20I TGGCCA 1 cut(s) 194
Mph1103I ATGCAT 1 cut(s) 533
MroNI GCCGGC 1 cut(s) 181
MroXI GAANNNNTTC 1 cut(s) 1386
MscI TGGCCA 1 cut(s) 194
MseI TTAA 1 cut(s) 1269
MslI CAYNNNNRTG 1 cut(s) 1301
Msp20I TGGCCA 1 cut(s) 194
MspA1I CMGCKG 2 cut(s) 694, 806
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 4 cut(s) 113, 203, 344, 1445
Mva1269I GAATGC 2 cut(s) 533, 1145
MvaI CCWGG 4 cut(s) 113, 203, 344, 1445
MvnI CGCG 4 cut(s) 20, 218, 233, 377
NaeI GCCGGC 1 cut(s) 183
NdeII GATC 2 cut(s) 259, 644
NgoMIV GCCGGC 1 cut(s) 181
NlaIII CATG 2 cut(s) 178, 591
NlaIV GGNNCC 3 cut(s) 470, 646, 1511
NmuCI GTSAC 3 cut(s) 394, 744, 1370
NsiI ATGCAT 1 cut(s) 533
PceI AGGCCT 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PctI GAATGC 2 cut(s) 533, 1145
PdiI GCCGGC 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 1386
PfeI GAWTC 4 cut(s) 426, 1002, 1306, 1382
PkrI GCNGC 6 cut(s) 160, 180, 805, 1354, 1474, 1505
PleI GAGTC 1 cut(s) 693
PpsI GAGTC 1 cut(s) 693
PsiI TTATAA 1 cut(s) 225
Psp6I CCWGG 4 cut(s) 111, 201, 342, 1443
PspEI GGTNACC 1 cut(s) 394
PspFI CCCAGC 2 cut(s) 694, 1181
PspGI CCWGG 4 cut(s) 111, 201, 342, 1443
PspN4I GGNNCC 3 cut(s) 470, 646, 1511
PsrI GAACNNNNNNTAC 4 cut(s) 101, 133, 1406, 1438
PstI CTGCAG 1 cut(s) 1354
PsuI RGATCY 2 cut(s) 259, 644
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 5 cut(s) 601, 980, 1409, 1415, 1466
RsaNI GTAC 5 cut(s) 600, 979, 1408, 1414, 1465
RseI CAYNNNNRTG 1 cut(s) 1301
SaqAI TTAA 1 cut(s) 1269
SatI GCNGC 6 cut(s) 159, 179, 804, 1353, 1473, 1504
Sau3AI GATC 2 cut(s) 259, 644
SchI GAGTC 1 cut(s) 693
ScrFI CCNGG 4 cut(s) 113, 203, 344, 1445
SduI GDGCHC 4 cut(s) 150, 297, 423, 554
SfaNI GCATC 3 cut(s) 57, 799, 1182
SfcI CTRYAG 1 cut(s) 1350
SfiI GGCCNNNNNGGCC 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 1301
SmlI CTYRAG 2 cut(s) 933, 1341
SmoI CTYRAG 2 cut(s) 933, 1341
Sse9I AATT 9 cut(s) 85, 128, 509, 893, 1205, 1221, 1255, 1395, 1428
SseBI AGGCCT 1 cut(s) 116
SsiI CCGC 7 cut(s) 159, 199, 392, 399, 692, 1070, 1503
SspI AATATT 1 cut(s) 1365
SspMI CTAG 2 cut(s) 516, 1500
StuI AGGCCT 1 cut(s) 116
StyD4I CCNGG 4 cut(s) 111, 201, 342, 1443
StyI CCWWGG 1 cut(s) 1377
TaaI ACNGT 4 cut(s) 463, 750, 970, 1135
TaiI ACGT 3 cut(s) 192, 578, 1460
TaqI TCGA 1 cut(s) 1187
TaqII GACCGA 1 cut(s) 732
TasI AATT 9 cut(s) 85, 128, 509, 893, 1205, 1221, 1255, 1395, 1428
TatI WGTACW 3 cut(s) 1407, 1413, 1464
TauI GCSGC 2 cut(s) 161, 1506
TfiI GAWTC 4 cut(s) 426, 1002, 1306, 1382
Tru1I TTAA 1 cut(s) 1269
Tru9I TTAA 1 cut(s) 1269
TscAI CASTG 3 cut(s) 1029, 1138, 1306
TseFI GTSAC 3 cut(s) 394, 744, 1370
TseI GCWGC 4 cut(s) 178, 803, 1352, 1472
Tsp45I GTSAC 3 cut(s) 394, 744, 1370
TspGWI ACGGA 3 cut(s) 142, 379, 637
TspRI CASTG 3 cut(s) 1029, 1138, 1306
XapI RAATTY 2 cut(s) 128, 1255
XmiI GTMKAC 2 cut(s) 72, 688
XmnI GAANNNNTTC 1 cut(s) 1386
XspI CTAG 2 cut(s) 516, 1500
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.