MD12G1186600.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017325
Physical Location & Seq
Forward (+)
26877240 .. 26878699
1460 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1186600.v1.1.491

Sequence Viewer

Length: 555 bp
ATGATCAGAGTTTACAGCAACAGCTTCTTTCCCGTCAGTGCTATACACATTTACAACCATATGCGGGAGATGGGTGTTGGATCGGATCATTTCACCTTCAATTTTGCGCTCAAAGCTTGCGCCAGAGTAATGGAGCAGATGGAAGATGGTGAGAAATCGTATGGATTTGGTATGGATCGAAAGGGCGGGGAAATTCATTGCCGGGTTTTGAAGTTGGGGAAATTCAATGATGGAACATTGAAGCTCTTCCGTGAAATGCAAGTTGCCGAGGTGGAAGCTGCCGAAGTAACGGTCATATCGATATTGGGTGCTTGTGCTGAAACGGGTGCACTGGAGATTGGAAGGAAGATTCACGAGTCCTTGAAGCACCATAATATTGGAGGGTGTTTGGGGGTTGCCCTTGTAGATACGTATTCGAAATGTGGAAAATTGAGTGTTGCTTGGGAAGTGTTTAGTGAGCTGAAAATGAAACCCGTCGGCTGCTGGGACTCGATGATTATGGGTTTGGCTGTCCATGGTTACTGCAACGACCAACGAGGCTCTGGAATTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

185

Amino Acids

20.3

Weight (kDa)

6.7

Isoelectric Point (pI)

22.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017325)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g10030
malus_domestica MD04G1173200.v1.1 MD12G1186600.v1.1
prunus_persica Prupe.6G292100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0460711
rosa_laevigata RLG00000024986
rosa_multiflora Rmu_sc0007459.1_g000001 Rmu_sc0011069.1_g000007
rosa_roxburghii Rroxscaffold_6G00419280
rosa_rugosa Rorug03G0045400
rosa_samantha Rh3AG102900 Rh3DG108100
rosa_wichuraiana Rw3G008760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 64, 186
AclWI GGATC 3 cut(s) 88, 93, 183
AcsI RAATTY 2 cut(s) 192, 221
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 5 cut(s) 100, 211, 226, 241, 364
AluBI AGCT 5 cut(s) 24, 116, 244, 278, 460
AluI AGCT 5 cut(s) 24, 116, 244, 278, 460
Alw21I GWGCWC 1 cut(s) 331
Alw44I GTGCAC 1 cut(s) 327
AlwI GGATC 3 cut(s) 88, 93, 183
ApaLI GTGCAC 1 cut(s) 327
ApeKI GCWGC 2 cut(s) 278, 480
ApoI RAATTY 2 cut(s) 192, 221
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 1 cut(s) 245
AspLEI GCGC 2 cut(s) 109, 122
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 2 cut(s) 85, 161
AsuII TTCGAA 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 331
BauI CACGAG 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 2 cut(s) 265, 467
BccI CCATC 4 cut(s) 64, 133, 140, 224
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 203
BisI GCNGC 2 cut(s) 279, 481
BlsI GCNGC 2 cut(s) 280, 482
Bme1390I CCNGG 1 cut(s) 203
BmrFI CCNGG 1 cut(s) 203
BpmI CTGGAG 1 cut(s) 353
Bpu14I TTCGAA 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 203
Bsa29I ATCGAT 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 411
BsaJI CCNNGG 2 cut(s) 267, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 336
Bse3DI GCAATG 1 cut(s) 196
BseCI ATCGAT 1 cut(s) 299
BseDI CCNNGG 2 cut(s) 267, 514
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 196
BseNI ACTGG 1 cut(s) 336
BseSI GKGCMC 1 cut(s) 331
BseXI GCAGC 2 cut(s) 265, 467
BseYI CCCAGC 1 cut(s) 483
BshVI ATCGAT 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 331
BsiSI CCGG 1 cut(s) 202
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 500
Bsp119I TTCGAA 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 331
Bsp143I GATC 4 cut(s) 3, 80, 85, 175
Bsp19I CCATGG 1 cut(s) 514
BspACI CCGC 2 cut(s) 64, 186
BspDI ATCGAT 1 cut(s) 299
BspPI GGATC 3 cut(s) 88, 93, 183
BspQI GCTCTTC 1 cut(s) 251
BspT104I TTCGAA 1 cut(s) 416
BsrDI GCAATG 1 cut(s) 196
BsrI ACTGG 1 cut(s) 336
BssECI CCNNGG 2 cut(s) 267, 514
BssMI GATC 4 cut(s) 3, 80, 85, 175
BssSI CACGAG 1 cut(s) 353
BssT1I CCWWGG 1 cut(s) 514
Bst2BI CACGAG 1 cut(s) 353
Bst4CI ACNGT 1 cut(s) 292
Bst6I CTCTTC 1 cut(s) 251
BstBAI YACGTR 1 cut(s) 411
BstBI TTCGAA 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 118
BstDSI CCRYGG 1 cut(s) 514
BstHHI GCGC 2 cut(s) 109, 122
BstKTI GATC 4 cut(s) 6, 83, 88, 178
BstMBI GATC 4 cut(s) 3, 80, 85, 175
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 201
BstSLI GKGCMC 1 cut(s) 331
BstSNI TACGTA 1 cut(s) 411
BstV1I GCAGC 2 cut(s) 265, 467
BstXI CCANNNNNNTGG 2 cut(s) 130, 377
Bsu15I ATCGAT 1 cut(s) 299
BsuTUI ATCGAT 1 cut(s) 299
BtgI CCRYGG 1 cut(s) 514
BtsIMutI CAGTG 2 cut(s) 43, 329
Cac8I GCNNGC 1 cut(s) 118
CfoI GCGC 2 cut(s) 109, 122
ClaI ATCGAT 1 cut(s) 299
CviAII CATG 1 cut(s) 515
CviJI RGCY 8 cut(s) 24, 116, 244, 278, 460, 480, 509, 540
CviKI_1 RGCY 8 cut(s) 24, 116, 244, 278, 460, 480, 509, 540
DpnI GATC 4 cut(s) 5, 82, 87, 177
DpnII GATC 4 cut(s) 3, 80, 85, 175
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Eco105I TACGTA 1 cut(s) 411
Eco130I CCWWGG 1 cut(s) 514
EcoT14I CCWWGG 1 cut(s) 514
ErhI CCWWGG 1 cut(s) 514
FaeI CATG 1 cut(s) 518
FaiI YATR 9 cut(s) 44, 60, 62, 162, 173, 296, 372, 500, 516
FaqI GGGAC 1 cut(s) 500
FatI CATG 1 cut(s) 514
FauI CCCGC 2 cut(s) 57, 179
FauNDI CATATG 1 cut(s) 60
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 279, 481
Fsp4HI GCNGC 2 cut(s) 279, 481
GlaI GCGC 2 cut(s) 108, 121
GluI GCNGC 2 cut(s) 279, 481
GsaI CCCAGC 1 cut(s) 487
GsuI CTGGAG 1 cut(s) 353
HapII CCGG 1 cut(s) 202
HhaI GCGC 2 cut(s) 109, 122
Hin1II CATG 1 cut(s) 518
Hin6I GCGC 2 cut(s) 107, 120
HinP1I GCGC 2 cut(s) 107, 120
HindIII AAGCTT 1 cut(s) 114
HinfI GANTC 3 cut(s) 349, 356, 488
HpaII CCGG 1 cut(s) 202
HphI GGTGA 2 cut(s) 85, 161
Hpy166II GTNNAC 2 cut(s) 13, 329
Hpy188I TCNGA 2 cut(s) 8, 85
Hpy188III TCNNGA 2 cut(s) 353, 543
Hpy8I GTNNAC 2 cut(s) 13, 329
Hpy99I CGWCG 1 cut(s) 479
HpyAV CCTTC 2 cut(s) 106, 336
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 410
HpyCH4V TGCA 3 cut(s) 259, 329, 525
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 410
Hsp92II CATG 1 cut(s) 518
HspAI GCGC 2 cut(s) 107, 120
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 80, 85, 175
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 1 cut(s) 133
LpnPI CCDG 5 cut(s) 136, 215, 317, 469, 528
Lsp1109I GCAGC 2 cut(s) 265, 467
MaeII ACGT 1 cut(s) 410
MaeIII GTNAC 2 cut(s) 286, 518
MalI GATC 4 cut(s) 5, 82, 87, 177
MboI GATC 4 cut(s) 3, 80, 85, 175
MboII GAAGA 3 cut(s) 155, 238, 358
MhlI GDGCHC 1 cut(s) 331
MluCI AATT 5 cut(s) 100, 192, 221, 428, 546
MlyI GAGTC 2 cut(s) 365, 482
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 3 cut(s) 262, 374, 530
MroXI GAANNNNTTC 1 cut(s) 245
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 113
NciI CCSGG 1 cut(s) 203
NcoI CCATGG 1 cut(s) 514
NdeI CATATG 1 cut(s) 60
NdeII GATC 4 cut(s) 3, 80, 85, 175
NlaIII CATG 1 cut(s) 518
NmeAIII GCCGAG 1 cut(s) 292
NspV TTCGAA 1 cut(s) 416
PciSI GCTCTTC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PdmI GAANNNNTTC 1 cut(s) 245
PfeI GAWTC 1 cut(s) 349
PkrI GCNGC 2 cut(s) 280, 482
PleI GAGTC 2 cut(s) 364, 482
PpsI GAGTC 2 cut(s) 364, 482
Ppu21I YACGTR 1 cut(s) 411
PspFI CCCAGC 1 cut(s) 483
SapI GCTCTTC 1 cut(s) 251
SatI GCNGC 2 cut(s) 279, 481
Sau3AI GATC 4 cut(s) 3, 80, 85, 175
SchI GAGTC 2 cut(s) 365, 482
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 1 cut(s) 331
SetI ASST 8 cut(s) 26, 98, 118, 246, 273, 280, 413, 462
SfuI TTCGAA 1 cut(s) 416
SnaBI TACGTA 1 cut(s) 411
Sse9I AATT 5 cut(s) 100, 192, 221, 428, 546
SsiI CCGC 2 cut(s) 64, 186
SspI AATATT 1 cut(s) 376
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 1 cut(s) 514
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 1 cut(s) 413
TaqI TCGA 4 cut(s) 178, 299, 416, 491
TasI AATT 5 cut(s) 100, 192, 221, 428, 546
TfiI GAWTC 1 cut(s) 349
TscAI CASTG 2 cut(s) 43, 336
TseI GCWGC 2 cut(s) 278, 480
TspDTI ATGAA 2 cut(s) 185, 482
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 2 cut(s) 43, 336
VneI GTGCAC 1 cut(s) 327
XapI RAATTY 2 cut(s) 192, 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.