MD12G1192900.v1.1

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011912
Physical Location & Seq
Forward (+)
27475936 .. 27480233
4298 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1192900.v1.1.491

Sequence Viewer

Length: 1797 bp
ATGAAAATGGAGTTCACATTTAGGAAGGTCCTATTATTTTCACTGTTCTCCCTAGTTCTGTTTGGATATGCTACATCAGAGTTGGAAAATGAAGTTTCACAAAAGCTGAGATCTCCTCCACACAAAAATGTGAGAAGTAATGTTATTGATGGCACTGGTCAGGAGAGTTCCTTAAATTTTGAGGTTTCTAAAAATGGACTGGGTGAGGCGAAGAGCGCAAACAGCAAAGTCTCAGTCTCTACTGTTGCGTTATTTACGTTGGCAATGGCTGCTGCTACAGGTTTAGGTGCGGTCCCATTCTTCTTTGTTGAGCTTGATCCACAATGGGCGGGATTATGCAATGGTATGGCAGCTGGTGTAATGTTGGCTGCAAGCTTCGATCTTATACAGGAAGGGCAAGGTCATGGTGCTGGGAACTGGGTCGTTCATGGGCTTTTAGCTGGGGGCCTTTTTATTTGGCTGTGCAAGCAGTTTCTTGAACACTATGGAGAGGTTAGTATGTTAGACATCAAAGGTGCAGATGCAACTAAAGTCGTTCTTGTGATTGGAATAATGACTCTTCATTCGTTCGGAGAGGGTTCTGGTGTTGGGGTTTCCTTTGCTGGCTCAAAGGGTTTCTCTCAGGGACTTTTGGTAACTTTGGCTATTGCTGTACACAACATACCAGAAGGCTTAGCTGTGAGTATGGTGCTTGCATCCAGGGGTGTTTCTCCGCAAAATGCCATGTTATGGAGCGTGATTACATCATTACCACAGCCCCTTGTAGCAGTTCCTTCTTTCATCTGCGCTGATGCATTTAGCAAGTTTCTTCCTTTCTGTACGGGCTTTGCTGCTGGATGTATGATCTGGATGGTTGTTGCTGAGGTGCTTCCTGATGCATTTAAGGAAGCCTCTCCATCGCAGGTTGCATCAGCAGCCACTCTCTCTGTAGCATTCATGGAAGCTCTTAGCACTCTTTTTCAGAATTTCACCCATGATTACAACTCAGAGGATGCTTCTGGCTTCTTTGTCTCATTACTTTTTGGTCTTGGACCATTACTCGGTGGCACTGTTCTTGTTGCATTTGCTTTAGCTTTTCATCTTCAGCATGCTCTTCTCATGGGTGCAGCATCTGGCATTGCCTTTGTTCTTGGTGCCTGGAGACCATTGCAGCTACTTTTTTCGTTAAAGATGGGATTTCTATCTACTATATTTCTGCTTGCAATGGGAGCTGGATCCGTCCATATTTTGAGCTCCAGTATTTTGAAGCTCGCAGGTCGCAAAAAAACTTCAGTTCAAAACTTACCCACAGCAAATGGTTTTCCAGTAAGCATTCACACCCTCCAGGCATTCTTGTCATGTGGAGCTGTTGCCTTCCATGCTTTGGCTGAAGGGCTTGCACTAGGGGTTGCTGCACCAAAAGCTTATGGACTTGGTCGGCACATGGTCCTTCCTGTCTCCCTACATGGACTCCCTCGAGGTGCAGCTGTGGCTAGCTGCATCTTTGGGGCTACTGATAACTGGCATGCGGCCCTTGTGGCGGCTGCTTTAATTGGATTTGTGGGTCCAATATCAGCAATAGGAGCAATACTTGCAGGAATTGACTATAGCGGATTAGACCATGTGATGGTTTTTGCTTGTGGGGGACTGCTCCCAAGTTTTGGAAATGTAGTAAAGAGAGCAGTGAAGTTGGATGTGCGGAAAAGTAGCTGCGGGATTTTGATTGGTGTGGCATTTGCAACTCTGTGCTTGACATGCACAAAGTTGGTGTGCTTGCACACACCTTACTGTAATTCTGCACCGGAGGCTGTTAGATGA

Protein Analysis

599

Amino Acids

62.34

Weight (kDa)

7.6

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 175 - 297 4.9e-12 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G08650 AT3G08650
fragaria_vesca FvH4_6g09450 FvH4_6g09450
malus_domestica MD04G1177600.v1.1 MD12G1192900.v1.1
prunus_persica Prupe.6G297500_v2.0.a1
pyrus_communis pycom04g15660 pycom12g17960
rosa_chinensis RchiOBHm_Chr3g0459921
rosa_laevigata RLG00000025051
rosa_multiflora Rmu_sc0000240.1_g000047 Rmu_sc0016534.1_g000002
rosa_roxburghii Rroxscaffold_6G00419910
rosa_rugosa Rorug03G0038800
rosa_samantha Rh3AG097400 Rh3BG100300 Rh3CG102100 Rh3DG101800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1455
Acc36I ACCTGC 2 cut(s) 892, 1244
AccB1I GGYRCC 1 cut(s) 1133
AccB7I CCANNNNNTGG 4 cut(s) 729, 1363, 1606, 1640
AciI CCGC 8 cut(s) 290, 329, 713, 1508, 1520, 1590, 1678, 1692
AclWI GGATC 3 cut(s) 311, 1209, 1222
AcsI RAATTY 2 cut(s) 175, 964
AcuI CTGAAG 3 cut(s) 1067, 1254, 1389
AfaI GTAC 2 cut(s) 654, 820
AfiI CCNNNNNNNGG 6 cut(s) 729, 1040, 1363, 1519, 1606, 1640
AgsI TTSAA 3 cut(s) 479, 1246, 1277
AjnI CCWGG 3 cut(s) 698, 1136, 1323
Alw21I GWGCWC 1 cut(s) 1235
Alw26I GTCTC 5 cut(s) 235, 241, 1015, 1135, 1441
AlwI GGATC 3 cut(s) 311, 1209, 1222
AlwNI CAGNNNCTG 1 cut(s) 1112
Ama87I CYCGRG 1 cut(s) 1455
AoxI GGCC 2 cut(s) 445, 1509
ApoI RAATTY 2 cut(s) 175, 964
ArsI GACNNNNNNTTYG 4 cut(s) 219, 251, 547, 579
AspLEI GCGC 2 cut(s) 218, 788
AspS9I GGNCC 7 cut(s) 28, 292, 445, 1031, 1426, 1510, 1544
AsuHPI GGTGA 2 cut(s) 215, 961
AsuNHI GCTAGC 1 cut(s) 1472
AvaI CYCGRG 1 cut(s) 1455
AvaII GGWCC 5 cut(s) 28, 292, 1031, 1426, 1544
BamHI GGATCC 1 cut(s) 1214
BanI GGYRCC 1 cut(s) 1133
BanII GRGCYC 1 cut(s) 1235
Bbv12I GWGCWC 1 cut(s) 1235
BbvCI CCTCAGC 1 cut(s) 861
BccI CCATC 5 cut(s) 143, 844, 904, 1165, 1600
BciT130I CCWGG 3 cut(s) 700, 1138, 1325
BcoDI GTCTC 5 cut(s) 235, 241, 1015, 1135, 1441
BfaI CTAG 3 cut(s) 53, 1382, 1473
BfmI CTRYAG 3 cut(s) 276, 927, 1585
BfuAI ACCTGC 2 cut(s) 892, 1244
BglI GCCNNNNNGGC 1 cut(s) 1517
BglII AGATCT 1 cut(s) 110
BlpI GCTNAGC 1 cut(s) 673
Bme1390I CCNGG 3 cut(s) 700, 1138, 1325
Bme18I GGWCC 5 cut(s) 28, 292, 1031, 1426, 1544
BmeT110I CYCGRG 1 cut(s) 1455
BmgT120I GGNCC 7 cut(s) 28, 292, 445, 1031, 1426, 1510, 1544
BmiI GGNNCC 5 cut(s) 294, 446, 1135, 1216, 1545
BmrFI CCNGG 3 cut(s) 700, 1138, 1325
BmrI ACTGGG 2 cut(s) 209, 427
BmsI GCATC 8 cut(s) 511, 704, 781, 865, 917, 982, 1118, 1488
BmtI GCTAGC 1 cut(s) 1476
BmuI ACTGGG 2 cut(s) 209, 427
BplI GAGNNNNNCTC 2 cut(s) 100, 132
BpmI CTGGAG 3 cut(s) 1159, 1219, 1307
Bpu10I CCTNAGC 1 cut(s) 861
Bpu1102I GCTNAGC 1 cut(s) 673
BsaBI GATNNNNATC 1 cut(s) 1180
BsaI GGTCTC 1 cut(s) 1135
BsaJI CCNNGG 1 cut(s) 699
BsaWI WCCGGW 1 cut(s) 1780
BsaXI ACNNNNNCTCC 2 cut(s) 1434, 1464
Bsc4I CCNNNNNNNGG 6 cut(s) 729, 1040, 1363, 1519, 1606, 1640
Bse1I ACTGG 6 cut(s) 160, 204, 422, 1236, 1304, 1505
Bse3DI GCAATG 5 cut(s) 270, 346, 1116, 1145, 1209
Bse8I GATNNNNATC 1 cut(s) 1180
BseBI CCWGG 3 cut(s) 700, 1138, 1325
BseDI CCNNGG 1 cut(s) 699
BseGI GGATG 5 cut(s) 695, 842, 855, 997, 1678
BseJI GATNNNNATC 1 cut(s) 1180
BseLI CCNNNNNNNGG 6 cut(s) 729, 1040, 1363, 1519, 1606, 1640
BseMI GCAATG 5 cut(s) 270, 346, 1116, 1145, 1209
BseMII CTCAG 5 cut(s) 98, 246, 635, 852, 999
BseNI ACTGG 6 cut(s) 160, 204, 422, 1236, 1304, 1505
BseRI GAGGAG 1 cut(s) 105
BseYI CCCAGC 2 cut(s) 410, 440
BsgI GTGCAG 5 cut(s) 537, 1125, 1377, 1482, 1761
BshFI GGCC 2 cut(s) 447, 1511
BshNI GGYRCC 1 cut(s) 1133
BsiHKAI GWGCWC 1 cut(s) 1235
BsiHKCI CYCGRG 1 cut(s) 1455
BsiSI CCGG 1 cut(s) 1781
BslFI GGGAC 3 cut(s) 278, 639, 1638
BslI CCNNNNNNNGG 6 cut(s) 729, 1040, 1363, 1519, 1606, 1640
BsmAI GTCTC 5 cut(s) 235, 241, 1015, 1135, 1441
BsmFI GGGAC 3 cut(s) 278, 639, 1638
BsmI GAATGC 3 cut(s) 932, 1311, 1328
BsnI GGCC 2 cut(s) 447, 1511
Bso31I GGTCTC 1 cut(s) 1135
BsoBI CYCGRG 1 cut(s) 1455
Bsp1286I GDGCHC 1 cut(s) 1235
Bsp1407I TGTACA 1 cut(s) 652
Bsp143I GATC 5 cut(s) 110, 316, 379, 843, 1214
Bsp1720I GCTNAGC 1 cut(s) 673
BspACI CCGC 8 cut(s) 290, 329, 713, 1508, 1520, 1590, 1678, 1692
BspANI GGCC 2 cut(s) 447, 1511
BspCNI CTCAG 5 cut(s) 99, 245, 634, 853, 998
BspLI GGNNCC 5 cut(s) 294, 446, 1135, 1216, 1545
BspMI ACCTGC 2 cut(s) 892, 1244
BspOI GCTAGC 1 cut(s) 1476
BspPI GGATC 3 cut(s) 311, 1209, 1222
BspQI GCTCTTC 2 cut(s) 206, 1098
BspT107I GGYRCC 1 cut(s) 1133
BspTNI GGTCTC 1 cut(s) 1135
BsrDI GCAATG 5 cut(s) 270, 346, 1116, 1145, 1209
BsrGI TGTACA 1 cut(s) 652
BsrI ACTGG 6 cut(s) 160, 204, 422, 1236, 1304, 1505
BssECI CCNNGG 1 cut(s) 699
BssMI GATC 5 cut(s) 110, 316, 379, 843, 1214
Bst2UI CCWGG 3 cut(s) 700, 1138, 1325
Bst4CI ACNGT 4 cut(s) 45, 244, 1051, 1769
Bst6I CTCTTC 3 cut(s) 206, 564, 1098
BstAPI GCANNNNNTGC 2 cut(s) 269, 1571
BstAUI TGTACA 1 cut(s) 652
BstDEI CTNAG 7 cut(s) 107, 232, 621, 673, 861, 947, 985
BstF5I GGATG 5 cut(s) 695, 842, 855, 997, 1678
BstHHI GCGC 2 cut(s) 218, 788
BstKTI GATC 5 cut(s) 113, 319, 382, 846, 1217
BstMAI GTCTC 5 cut(s) 235, 241, 1015, 1135, 1441
BstMBI GATC 5 cut(s) 110, 316, 379, 843, 1214
BstNI CCWGG 3 cut(s) 700, 1138, 1325
BstNSI RCATGY 3 cut(s) 1091, 1508, 1737
BstSCI CCNGG 3 cut(s) 698, 1136, 1323
BstSFI CTRYAG 3 cut(s) 276, 927, 1585
BstX2I RGATCY 2 cut(s) 110, 1214
BstYI RGATCY 2 cut(s) 110, 1214
BsuRI GGCC 2 cut(s) 447, 1511
BtgZI GCGATG 1 cut(s) 882
BtsCI GGATG 5 cut(s) 695, 842, 855, 997, 1678
BtsI GCAGTG 1 cut(s) 1668
BtsIMutI CAGTG 4 cut(s) 41, 153, 1047, 1668
BveI ACCTGC 2 cut(s) 892, 1244
CaiI CAGNNNCTG 1 cut(s) 1112
CfoI GCGC 2 cut(s) 218, 788
Cfr13I GGNCC 7 cut(s) 28, 292, 445, 1031, 1426, 1510, 1544
Csp6I GTAC 2 cut(s) 653, 819
CviQI GTAC 2 cut(s) 653, 819
DdeI CTNAG 7 cut(s) 107, 232, 621, 673, 861, 947, 985
DpnI GATC 5 cut(s) 112, 318, 381, 845, 1216
DpnII GATC 5 cut(s) 110, 316, 379, 843, 1214
Eam1104I CTCTTC 3 cut(s) 206, 564, 1098
EarI CTCTTC 3 cut(s) 206, 564, 1098
Ecl136II GAGCTC 1 cut(s) 1233
Eco24I GRGCYC 1 cut(s) 1235
Eco31I GGTCTC 1 cut(s) 1135
Eco47I GGWCC 5 cut(s) 28, 292, 1031, 1426, 1544
Eco53kI GAGCTC 1 cut(s) 1233
Eco57I CTGAAG 3 cut(s) 1067, 1254, 1389
Eco88I CYCGRG 1 cut(s) 1455
EcoICRI GAGCTC 1 cut(s) 1233
EcoO109I RGGNCCY 2 cut(s) 28, 445
EcoRII CCWGG 3 cut(s) 698, 1136, 1323
EcoT22I ATGCAT 2 cut(s) 796, 880
EcoT38I GRGCYC 1 cut(s) 1235
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 3 cut(s) 278, 639, 1638
FauI CCCGC 2 cut(s) 322, 1685
FokI GGATG 5 cut(s) 682, 849, 862, 1004, 1685
FriOI GRGCYC 1 cut(s) 1235
FspBI CTAG 3 cut(s) 53, 1382, 1473
GlaI GCGC 2 cut(s) 217, 787
GsaI CCCAGC 2 cut(s) 414, 444
GsuI CTGGAG 3 cut(s) 1159, 1219, 1307
HaeIII GGCC 2 cut(s) 447, 1511
HapII CCGG 1 cut(s) 1781
HhaI GCGC 2 cut(s) 218, 788
Hin6I GCGC 2 cut(s) 216, 786
HinP1I GCGC 2 cut(s) 216, 786
HindIII AAGCTT 2 cut(s) 373, 1401
HinfI GANTC 2 cut(s) 556, 1449
HpaII CCGG 1 cut(s) 1781
HphI GGTGA 2 cut(s) 215, 961
Hpy166II GTNNAC 2 cut(s) 15, 655
Hpy188I TCNGA 4 cut(s) 79, 572, 963, 988
Hpy188III TCNNGA 4 cut(s) 161, 476, 847, 872
Hpy8I GTNNAC 2 cut(s) 15, 655
HpyAV CCTTC 7 cut(s) 19, 386, 662, 783, 1363, 1364, 1439
HpyCH4III ACNGT 4 cut(s) 45, 244, 1051, 1769
HpyCH4IV ACGT 1 cut(s) 257
HpyF3I CTNAG 7 cut(s) 107, 232, 621, 673, 861, 947, 985
HpySE526I ACGT 1 cut(s) 257
HspAI GCGC 2 cut(s) 216, 786
Kzo9I GATC 5 cut(s) 110, 316, 379, 843, 1214
LguI GCTCTTC 2 cut(s) 206, 1098
LmnI GCTCC 6 cut(s) 732, 1208, 1238, 1343, 1562, 1635
LweI GCATC 8 cut(s) 511, 704, 781, 865, 917, 982, 1118, 1488
MaeI CTAG 3 cut(s) 53, 1382, 1473
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 1 cut(s) 634
MalI GATC 5 cut(s) 112, 318, 381, 845, 1216
MboI GATC 5 cut(s) 110, 316, 379, 843, 1214
MboII GAAGA 6 cut(s) 223, 292, 551, 800, 1073, 1085
MflI RGATCY 2 cut(s) 110, 1214
MhlI GDGCHC 1 cut(s) 1235
MluCI AATT 5 cut(s) 175, 964, 1530, 1578, 1771
MlyI GAGTC 2 cut(s) 550, 1443
MmeI TCCRAC 2 cut(s) 63, 1650
Mph1103I ATGCAT 2 cut(s) 796, 880
MseI TTAA 4 cut(s) 173, 882, 1166, 1529
MslI CAYNNNNRTG 1 cut(s) 126
MspA1I CMGCKG 2 cut(s) 353, 1466
MspI CCGG 1 cut(s) 1781
MspR9I CCNGG 3 cut(s) 700, 1138, 1325
Mva1269I GAATGC 3 cut(s) 932, 1311, 1328
MvaI CCWGG 3 cut(s) 700, 1138, 1325
NdeII GATC 5 cut(s) 110, 316, 379, 843, 1214
NheI GCTAGC 1 cut(s) 1472
NlaIV GGNNCC 5 cut(s) 294, 446, 1135, 1216, 1545
NsiI ATGCAT 2 cut(s) 796, 880
NspI RCATGY 3 cut(s) 1091, 1508, 1737
PaeI GCATGC 2 cut(s) 1091, 1508
PaeR7I CTCGAG 1 cut(s) 1455
PciSI GCTCTTC 2 cut(s) 206, 1098
PctI GAATGC 3 cut(s) 932, 1311, 1328
PflFI GACNNNGTC 1 cut(s) 1413
PflMI CCANNNNNTGG 4 cut(s) 729, 1363, 1606, 1640
PleI GAGTC 2 cut(s) 550, 1443
PpsI GAGTC 2 cut(s) 550, 1443
PpuMI RGGWCCY 1 cut(s) 28
Psp124BI GAGCTC 1 cut(s) 1235
Psp5II RGGWCCY 1 cut(s) 28
Psp6I CCWGG 3 cut(s) 698, 1136, 1323
PspFI CCCAGC 2 cut(s) 410, 440
PspGI CCWGG 3 cut(s) 698, 1136, 1323
PspN4I GGNNCC 5 cut(s) 294, 446, 1135, 1216, 1545
PspPI GGNCC 7 cut(s) 28, 292, 445, 1031, 1426, 1510, 1544
PspPPI RGGWCCY 1 cut(s) 28
PspXI VCTCGAGB 1 cut(s) 1455
PstNI CAGNNNCTG 1 cut(s) 1112
PsuI RGATCY 2 cut(s) 110, 1214
PsyI GACNNNGTC 1 cut(s) 1413
PvuII CAGCTG 2 cut(s) 353, 1466
RsaI GTAC 2 cut(s) 654, 820
RsaNI GTAC 2 cut(s) 653, 819
RseI CAYNNNNRTG 1 cut(s) 126
SacI GAGCTC 1 cut(s) 1235
SapI GCTCTTC 2 cut(s) 206, 1098
SaqAI TTAA 4 cut(s) 173, 882, 1166, 1529
Sau3AI GATC 5 cut(s) 110, 316, 379, 843, 1214
Sau96I GGNCC 7 cut(s) 28, 292, 445, 1031, 1426, 1510, 1544
SchI GAGTC 2 cut(s) 550, 1443
ScrFI CCNGG 3 cut(s) 700, 1138, 1325
SduI GDGCHC 1 cut(s) 1235
SfaNI GCATC 8 cut(s) 511, 704, 781, 865, 917, 982, 1118, 1488
SfcI CTRYAG 3 cut(s) 276, 927, 1585
Sfr274I CTCGAG 1 cut(s) 1455
SinI GGWCC 5 cut(s) 28, 292, 1031, 1426, 1544
SlaI CTCGAG 1 cut(s) 1455
SmiMI CAYNNNNRTG 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 1455
SmoI CTYRAG 1 cut(s) 1455
SphI GCATGC 2 cut(s) 1091, 1508
Sse9I AATT 5 cut(s) 175, 964, 1530, 1578, 1771
SsiI CCGC 8 cut(s) 290, 329, 713, 1508, 1520, 1590, 1678, 1692
SspMI CTAG 3 cut(s) 53, 1382, 1473
SstI GAGCTC 1 cut(s) 1235
StyD4I CCNGG 3 cut(s) 698, 1136, 1323
TaaI ACNGT 4 cut(s) 45, 244, 1051, 1769
TaiI ACGT 1 cut(s) 260
TaqI TCGA 2 cut(s) 378, 1456
TasI AATT 5 cut(s) 175, 964, 1530, 1578, 1771
TatI WGTACW 1 cut(s) 652
TauI GCSGC 2 cut(s) 1511, 1523
Tru1I TTAA 4 cut(s) 173, 882, 1166, 1529
Tru9I TTAA 4 cut(s) 173, 882, 1166, 1529
TscAI CASTG 4 cut(s) 48, 160, 1054, 1668
TspDTI ATGAA 7 cut(s) 17, 105, 416, 551, 769, 925, 1067
TspGWI ACGGA 1 cut(s) 1207
TspRI CASTG 4 cut(s) 48, 160, 1054, 1668
Tth111I GACNNNGTC 1 cut(s) 1413
Van91I CCANNNNNTGG 4 cut(s) 729, 1363, 1606, 1640
VpaK11BI GGWCC 5 cut(s) 28, 292, 1031, 1426, 1544
XapI RAATTY 2 cut(s) 175, 964
XceI RCATGY 3 cut(s) 1091, 1508, 1737
XhoI CTCGAG 1 cut(s) 1455
XspI CTAG 3 cut(s) 53, 1382, 1473
Zsp2I ATGCAT 2 cut(s) 796, 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.