Rh3BG100300

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0011912
Physical Location & Seq
Reverse (-)
7995987 .. 8000343
4357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG100300.1

Sequence Viewer

Length: 1791 bp
ATGAAGTCTGCTTGTAGGAAAGCTGTGTTGTTTTCACTCCTCTTCGTGGTTCTATTTGGGTATGCTACAGCCGAGTCTGAAGAAGAGGTTTCACGGAAGTTGATATCTGCTCCGCACAAGAATGTGAGAACTAATGTTATTGATGGCACTGGTAATGAGAATACCTTAGGTTTTGAGGATATAAATAATGGGATCGGTGAGACAAAGGGCGGGAACAGCAAGGTCTCAATCTCCACTGTTGCATTGTTTACGCTGGCAATGGCTGCTGCCACTGGTTTAGGTGCAGTGCCATTCTTCTTTGTGGAGCTTGATCCGCAATGGGCTGGACTCTGCAATGGAATGGCTGCTGGTGTGATGTTGGCTGCGAGCTTTGATCTTATACAGGAAGGGCAAGGTCATGGAGCAGGGAATTGGGTGGTCTTTGGGATTCTAGCTGGGGGAATTTTTATTTTGCTTTGCAAGAAGTTTCTCGAACAATATGGAGAGGTTAGTATGTTGGACATTAAAGGTGCAGATGCAACTAAAGTAGTTCTTGTGATTGGAATAATGACTCTTCATTCATTTGGGGAGGGCTCTGGTGTTGGGGTTTCCTTTGCTGGTTCAAAGGGTTTCTCTCAGGGCCTTTTGGTAACTTTGGCTATTGCTGTTCACAATATACCAGAAGGATTAGCTGTAAGTATGGTGCTTGCATCAAGGGGTGTTTCACCACAGAATGCCATGTTATGGAGTGTAATTACGTCATTACCGCAGCCAATTGTAGCAGTTCCTTCATTCATGTGCGCTGATGCATTTAGCAAGTTCCTTCCTTTCTGTACGGGATTTGCTGCTGGATGTATGATCTGGATGGTTGTTGCAGAGGTGCTTCCTGATGCATTTAAGGAAGCCTCTCCATCACATGTTGCATCTGCAGCTACTCTCTCTGTTGCATTCATGGAAGCACTTAGTACTCTTTTTCAGAGTTTCAGCCATGACTACAGTTCAGAGGATGCTTCTGGCTTCTTTGTTTCATTGCTTTTTGGTCTTGGGCCATTGCTTGGTGGCATGGTCCTTGTTGCATTTGCACTTGCTTTCCGTTTCCAGCATGCTCTTCTCATGGGTGCGGCATCTGGCATTGCCTTTGTCCTTGGTGCCTGGCGACCATCGCAGCTACTTTTCTCCTCAAAGATGGGATTTTTTCCTCTTATTTTGCTGCTTGCCTTGGGAGCTGCATCTGTCCATACTTTGAGCTCTAGTATTCTGAAGCTTGCAGGTCGCAAAAAAACTTCAGTGCATAATTTGCCGACAGTAACTGGTTTCCCCGTGAGTGTTCACACCCTCCAGTCATTCTTGTCATGTGGAGCAGTTGCTCTTCACGCTTTGGCTGAAGGGCTTGCACTAGGGGTTGCTGCTCCAAAAGCTTATGGACTTGGTCGGCATATGGTTCTTCCTGTCTCCCTCCATGGACTCCCTCGAGGTGCAGCTGTGGCTAGCTGCATCTTCGGGGCTACAGATAACTGGCAGGGGTCCCTTGCGGCTGCTGCTTTAATTGGATTCATGGGTCCAATATCTGCAATTGGAGCAATACTTGCAGGAATTGACTATAGCGGTCTAGACCATGTCATGGTGTTTGCTTGTGGGGGACTACTCCCAAGTTTTGGAAATGTAGTAAGGAGAGCAGGGAGATTGGATATGCGAAAAAGCAGTTGTGGGCTTCTGATGGGTATGGTTTTTGCAACTTTGTGTTTGACTTGCACCAAGTTGGTTTGCTTGCACACACCTTATTGCAATTCTGCACCTGAGGCTGTCAGATAG

Protein Analysis

596

Amino Acids

61.89

Weight (kDa)

7.57

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 173 - 297 2.3e-12 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G08650 AT3G08650
fragaria_vesca FvH4_6g09450 FvH4_6g09450
malus_domestica MD04G1177600.v1.1 MD12G1192900.v1.1
prunus_persica Prupe.6G297500_v2.0.a1
pyrus_communis pycom04g15660 pycom12g17960
rosa_chinensis RchiOBHm_Chr3g0459921
rosa_laevigata RLG00000025051
rosa_multiflora Rmu_sc0000240.1_g000047 Rmu_sc0016534.1_g000002
rosa_roxburghii Rroxscaffold_6G00419910
rosa_rugosa Rorug03G0038800
rosa_samantha Rh3AG097400 Rh3BG100300 Rh3CG102100 Rh3DG101800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1449
Acc36I ACCTGC 1 cut(s) 1238
AccB1I GGYRCC 1 cut(s) 1127
AccB7I CCANNNNNTGG 4 cut(s) 723, 1034, 1600, 1634
AciI CCGC 7 cut(s) 113, 210, 314, 746, 1100, 1511, 1584
AclWI GGATC 2 cut(s) 200, 305
AcsI RAATTY 1 cut(s) 441
AcuI CTGAAG 4 cut(s) 99, 1248, 1259, 1383
AfaI GTAC 2 cut(s) 814, 946
AfiI CCNNNNNNNGG 5 cut(s) 46, 723, 1034, 1600, 1634
AflIII ACRYGT 1 cut(s) 895
AgsI TTSAA 1 cut(s) 603
AjnI CCWGG 1 cut(s) 1130
Alw21I GWGCWC 1 cut(s) 1229
Alw26I GTCTC 3 cut(s) 194, 229, 1435
AlwI GGATC 2 cut(s) 200, 305
AlwNI CAGNNNCTG 1 cut(s) 1289
Ama87I CYCGRG 1 cut(s) 1449
AoxI GGCC 2 cut(s) 619, 1025
ApoI RAATTY 1 cut(s) 441
AspLEI GCGC 1 cut(s) 782
AspS9I GGNCC 5 cut(s) 619, 1025, 1045, 1503, 1538
AsuHPI GGTGA 2 cut(s) 209, 696
AsuNHI GCTAGC 1 cut(s) 1466
AvaI CYCGRG 1 cut(s) 1449
AvaII GGWCC 3 cut(s) 1045, 1503, 1538
AxyI CCTNAGG 2 cut(s) 166, 1776
BanI GGYRCC 1 cut(s) 1127
BanII GRGCYC 2 cut(s) 575, 1229
Bbv12I GWGCWC 1 cut(s) 1229
BccI CCATC 6 cut(s) 137, 838, 898, 1147, 1159, 1690
BciT130I CCWGG 1 cut(s) 1132
BcoDI GTCTC 3 cut(s) 194, 229, 1435
BfaI CTAG 5 cut(s) 431, 1230, 1376, 1467, 1589
BfmI CTRYAG 5 cut(s) 66, 906, 973, 1485, 1579
BfuAI ACCTGC 1 cut(s) 1238
BmcAI AGTACT 1 cut(s) 946
Bme1390I CCNGG 1 cut(s) 1132
Bme18I GGWCC 3 cut(s) 1045, 1503, 1538
BmeT110I CYCGRG 1 cut(s) 1449
BmgT120I GGNCC 5 cut(s) 619, 1025, 1045, 1503, 1538
BmiI GGNNCC 4 cut(s) 1129, 1504, 1505, 1539
BmrFI CCNGG 1 cut(s) 1132
BmsI GCATC 9 cut(s) 505, 698, 775, 859, 911, 976, 1112, 1217, 1482
BmtI GCTAGC 1 cut(s) 1470
BpmI CTGGAG 1 cut(s) 1301
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 3 cut(s) 1123, 1197, 1438
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 723, 1034, 1600, 1634
Bse1I ACTGG 5 cut(s) 154, 277, 1294, 1318, 1499
Bse21I CCTNAGG 2 cut(s) 166, 1776
Bse3DI GCAATG 6 cut(s) 264, 323, 340, 1007, 1028, 1110
BseBI CCWGG 1 cut(s) 1132
BseDI CCNNGG 3 cut(s) 1123, 1197, 1438
BseGI GGATG 3 cut(s) 836, 849, 991
BseLI CCNNNNNNNGG 5 cut(s) 46, 723, 1034, 1600, 1634
BseMI GCAATG 6 cut(s) 264, 323, 340, 1007, 1028, 1110
BseMII CTCAG 2 cut(s) 629, 1767
BseNI ACTGG 5 cut(s) 154, 277, 1294, 1318, 1499
BseRI GAGGAG 2 cut(s) 29, 1147
BseYI CCCAGC 1 cut(s) 434
BsgI GTGCAG 4 cut(s) 303, 531, 1476, 1755
BshFI GGCC 2 cut(s) 621, 1027
BshNI GGYRCC 1 cut(s) 1127
BsiHKAI GWGCWC 1 cut(s) 1229
BsiHKCI CYCGRG 1 cut(s) 1449
BslFI GGGAC 2 cut(s) 1489, 1632
BslI CCNNNNNNNGG 5 cut(s) 46, 723, 1034, 1600, 1634
BsmAI GTCTC 3 cut(s) 194, 229, 1435
BsmFI GGGAC 2 cut(s) 1489, 1632
BsmI GAATGC 2 cut(s) 718, 926
BsnI GGCC 2 cut(s) 621, 1027
Bso31I GGTCTC 1 cut(s) 229
BsoBI CYCGRG 1 cut(s) 1449
Bsp1286I GDGCHC 2 cut(s) 575, 1229
Bsp143I GATC 4 cut(s) 192, 310, 373, 837
Bsp19I CCATGG 1 cut(s) 1438
BspACI CCGC 7 cut(s) 113, 210, 314, 746, 1100, 1511, 1584
BspANI GGCC 2 cut(s) 621, 1027
BspCNI CTCAG 2 cut(s) 628, 1768
BspLI GGNNCC 4 cut(s) 1129, 1504, 1505, 1539
BspMAI CTGCAG 1 cut(s) 910
BspMI ACCTGC 1 cut(s) 1238
BspOI GCTAGC 1 cut(s) 1470
BspPI GGATC 2 cut(s) 200, 305
BspQI GCTCTTC 2 cut(s) 1092, 1353
BspT107I GGYRCC 1 cut(s) 1127
BspTNI GGTCTC 1 cut(s) 229
BsrDI GCAATG 6 cut(s) 264, 323, 340, 1007, 1028, 1110
BsrI ACTGG 5 cut(s) 154, 277, 1294, 1318, 1499
BssECI CCNNGG 3 cut(s) 1123, 1197, 1438
BssMI GATC 4 cut(s) 192, 310, 373, 837
BssT1I CCWWGG 3 cut(s) 1123, 1197, 1438
Bst2UI CCWGG 1 cut(s) 1132
Bst4CI ACNGT 3 cut(s) 238, 977, 1285
Bst6I CTCTTC 5 cut(s) 47, 78, 558, 1092, 1353
BstAPI GCANNNNNTGC 3 cut(s) 263, 1276, 1565
BstC8I GCNNGC 9 cut(s) 255, 367, 687, 1083, 1194, 1245, 1371, 1468, 1748
BstDEI CTNAG 4 cut(s) 166, 615, 941, 1776
BstDSI CCRYGG 1 cut(s) 1438
BstF5I GGATG 3 cut(s) 836, 849, 991
BstHHI GCGC 1 cut(s) 782
BstKTI GATC 4 cut(s) 195, 313, 376, 840
BstMAI GTCTC 3 cut(s) 194, 229, 1435
BstMBI GATC 4 cut(s) 192, 310, 373, 837
BstNI CCWGG 1 cut(s) 1132
BstNSI RCATGY 2 cut(s) 899, 1085
BstSCI CCNGG 1 cut(s) 1130
BstSFI CTRYAG 5 cut(s) 66, 906, 973, 1485, 1579
Bsu36I CCTNAGG 2 cut(s) 166, 1776
BsuRI GGCC 2 cut(s) 621, 1027
BtgI CCRYGG 1 cut(s) 1438
BtgZI GCGATG 1 cut(s) 1125
BtsCI GGATG 3 cut(s) 836, 849, 991
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 5 cut(s) 147, 234, 270, 291, 1272
BveI ACCTGC 1 cut(s) 1238
Cac8I GCNNGC 9 cut(s) 255, 367, 687, 1083, 1194, 1245, 1371, 1468, 1748
CaiI CAGNNNCTG 1 cut(s) 1289
CfoI GCGC 1 cut(s) 782
Cfr13I GGNCC 5 cut(s) 619, 1025, 1045, 1503, 1538
Csp6I GTAC 2 cut(s) 813, 945
CviQI GTAC 2 cut(s) 813, 945
DdeI CTNAG 4 cut(s) 166, 615, 941, 1776
DpnI GATC 4 cut(s) 194, 312, 375, 839
DpnII GATC 4 cut(s) 192, 310, 373, 837
Eam1104I CTCTTC 5 cut(s) 47, 78, 558, 1092, 1353
EarI CTCTTC 5 cut(s) 47, 78, 558, 1092, 1353
Ecl136II GAGCTC 1 cut(s) 1227
Eco130I CCWWGG 3 cut(s) 1123, 1197, 1438
Eco24I GRGCYC 2 cut(s) 575, 1229
Eco31I GGTCTC 1 cut(s) 229
Eco32I GATATC 1 cut(s) 105
Eco47I GGWCC 3 cut(s) 1045, 1503, 1538
Eco53kI GAGCTC 1 cut(s) 1227
Eco57I CTGAAG 4 cut(s) 99, 1248, 1259, 1383
Eco81I CCTNAGG 2 cut(s) 166, 1776
Eco88I CYCGRG 1 cut(s) 1449
EcoICRI GAGCTC 1 cut(s) 1227
EcoO109I RGGNCCY 2 cut(s) 619, 1503
EcoRII CCWGG 1 cut(s) 1130
EcoRV GATATC 1 cut(s) 105
EcoT14I CCWWGG 3 cut(s) 1123, 1197, 1438
EcoT22I ATGCAT 2 cut(s) 790, 874
EcoT38I GRGCYC 2 cut(s) 575, 1229
ErhI CCWWGG 3 cut(s) 1123, 1197, 1438
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 2 cut(s) 1489, 1632
FauI CCCGC 1 cut(s) 203
FauNDI CATATG 1 cut(s) 1416
FokI GGATG 3 cut(s) 843, 856, 998
FriOI GRGCYC 2 cut(s) 575, 1229
FspBI CTAG 5 cut(s) 431, 1230, 1376, 1467, 1589
GlaI GCGC 1 cut(s) 781
GsaI CCCAGC 1 cut(s) 438
GsuI CTGGAG 1 cut(s) 1301
HaeIII GGCC 2 cut(s) 621, 1027
HhaI GCGC 1 cut(s) 782
Hin6I GCGC 1 cut(s) 780
HinP1I GCGC 1 cut(s) 780
HindIII AAGCTT 2 cut(s) 1241, 1395
HinfI GANTC 6 cut(s) 74, 327, 427, 550, 1443, 1530
HphI GGTGA 2 cut(s) 209, 696
Hpy166II GTNNAC 3 cut(s) 249, 649, 1309
Hpy188I TCNGA 6 cut(s) 79, 957, 982, 1239, 1695, 1787
Hpy188III TCNNGA 4 cut(s) 470, 841, 866, 1589
Hpy8I GTNNAC 3 cut(s) 249, 649, 1309
HpyAV CCTTC 5 cut(s) 380, 656, 777, 812, 1358
HpyCH4III ACNGT 3 cut(s) 238, 977, 1285
HpyCH4IV ACGT 1 cut(s) 737
HpyF3I CTNAG 4 cut(s) 166, 615, 941, 1776
HpySE526I ACGT 1 cut(s) 737
HspAI GCGC 1 cut(s) 780
KflI GGGWCCC 1 cut(s) 1503
Kzo9I GATC 4 cut(s) 192, 310, 373, 837
LguI GCTCTTC 2 cut(s) 1092, 1353
LmnI GCTCC 7 cut(s) 115, 304, 401, 1202, 1337, 1393, 1556
LweI GCATC 9 cut(s) 505, 698, 775, 859, 911, 976, 1112, 1217, 1482
MaeI CTAG 5 cut(s) 431, 1230, 1376, 1467, 1589
MaeII ACGT 1 cut(s) 737
MaeIII GTNAC 2 cut(s) 628, 1285
MalI GATC 4 cut(s) 194, 312, 375, 839
MboI GATC 4 cut(s) 192, 310, 373, 837
MboII GAAGA 9 cut(s) 34, 92, 95, 286, 545, 1079, 1340, 1415, 1468
MfeI CAATTG 2 cut(s) 753, 1551
MhlI GDGCHC 2 cut(s) 575, 1229
MluCI AATT 9 cut(s) 409, 441, 732, 753, 1273, 1524, 1551, 1572, 1765
MlyI GAGTC 4 cut(s) 83, 321, 544, 1437
MmeI TCCRAC 1 cut(s) 477
Mph1103I ATGCAT 2 cut(s) 790, 874
MseI TTAA 3 cut(s) 504, 876, 1523
MslI CAYNNNNRTG 2 cut(s) 120, 775
MspA1I CMGCKG 1 cut(s) 1460
MspR9I CCNGG 1 cut(s) 1132
MunI CAATTG 2 cut(s) 753, 1551
Mva1269I GAATGC 2 cut(s) 718, 926
MvaI CCWGG 1 cut(s) 1132
NcoI CCATGG 1 cut(s) 1438
NdeI CATATG 1 cut(s) 1416
NdeII GATC 4 cut(s) 192, 310, 373, 837
NheI GCTAGC 1 cut(s) 1466
NlaIV GGNNCC 4 cut(s) 1129, 1504, 1505, 1539
NmeAIII GCCGAG 1 cut(s) 97
NsiI ATGCAT 2 cut(s) 790, 874
NspI RCATGY 2 cut(s) 899, 1085
PaeI GCATGC 1 cut(s) 1085
PaeR7I CTCGAG 1 cut(s) 1449
PciI ACATGT 1 cut(s) 895
PciSI GCTCTTC 2 cut(s) 1092, 1353
PctI GAATGC 2 cut(s) 718, 926
PfeI GAWTC 2 cut(s) 427, 1530
PflFI GACNNNGTC 2 cut(s) 1407, 1595
PflMI CCANNNNNTGG 4 cut(s) 723, 1034, 1600, 1634
PleI GAGTC 4 cut(s) 82, 321, 544, 1437
PpsI GAGTC 4 cut(s) 82, 321, 544, 1437
PpuMI RGGWCCY 1 cut(s) 1503
PscI ACATGT 1 cut(s) 895
Psp124BI GAGCTC 1 cut(s) 1229
Psp5II RGGWCCY 1 cut(s) 1503
Psp6I CCWGG 1 cut(s) 1130
PspFI CCCAGC 1 cut(s) 434
PspGI CCWGG 1 cut(s) 1130
PspN4I GGNNCC 4 cut(s) 1129, 1504, 1505, 1539
PspPI GGNCC 5 cut(s) 619, 1025, 1045, 1503, 1538
PspPPI RGGWCCY 1 cut(s) 1503
PspXI VCTCGAGB 1 cut(s) 1449
PstI CTGCAG 1 cut(s) 910
PstNI CAGNNNCTG 1 cut(s) 1289
PsyI GACNNNGTC 2 cut(s) 1407, 1595
PvuII CAGCTG 1 cut(s) 1460
RsaI GTAC 2 cut(s) 814, 946
RsaNI GTAC 2 cut(s) 813, 945
RseI CAYNNNNRTG 2 cut(s) 120, 775
SacI GAGCTC 1 cut(s) 1229
SapI GCTCTTC 2 cut(s) 1092, 1353
SaqAI TTAA 3 cut(s) 504, 876, 1523
Sau3AI GATC 4 cut(s) 192, 310, 373, 837
Sau96I GGNCC 5 cut(s) 619, 1025, 1045, 1503, 1538
ScaI AGTACT 1 cut(s) 946
SchI GAGTC 4 cut(s) 83, 321, 544, 1437
ScrFI CCNGG 1 cut(s) 1132
SduI GDGCHC 2 cut(s) 575, 1229
SfaNI GCATC 9 cut(s) 505, 698, 775, 859, 911, 976, 1112, 1217, 1482
SfcI CTRYAG 5 cut(s) 66, 906, 973, 1485, 1579
Sfr274I CTCGAG 1 cut(s) 1449
SinI GGWCC 3 cut(s) 1045, 1503, 1538
SlaI CTCGAG 1 cut(s) 1449
SmiMI CAYNNNNRTG 2 cut(s) 120, 775
SmlI CTYRAG 1 cut(s) 1449
SmoI CTYRAG 1 cut(s) 1449
SphI GCATGC 1 cut(s) 1085
Sse9I AATT 9 cut(s) 409, 441, 732, 753, 1273, 1524, 1551, 1572, 1765
SsiI CCGC 7 cut(s) 113, 210, 314, 746, 1100, 1511, 1584
SspMI CTAG 5 cut(s) 431, 1230, 1376, 1467, 1589
SstI GAGCTC 1 cut(s) 1229
StyD4I CCNGG 1 cut(s) 1130
StyI CCWWGG 3 cut(s) 1123, 1197, 1438
TaaI ACNGT 3 cut(s) 238, 977, 1285
TaiI ACGT 1 cut(s) 740
TaqI TCGA 2 cut(s) 471, 1450
TasI AATT 9 cut(s) 409, 441, 732, 753, 1273, 1524, 1551, 1572, 1765
TatI WGTACW 1 cut(s) 944
TauI GCSGC 2 cut(s) 1103, 1514
TfiI GAWTC 2 cut(s) 427, 1530
Tru1I TTAA 3 cut(s) 504, 876, 1523
Tru9I TTAA 3 cut(s) 504, 876, 1523
TscAI CASTG 5 cut(s) 154, 241, 277, 291, 1272
TspDTI ATGAA 8 cut(s) 17, 545, 549, 759, 763, 919, 996, 1522
TspGWI ACGGA 2 cut(s) 109, 1061
TspRI CASTG 5 cut(s) 154, 241, 277, 291, 1272
Tth111I GACNNNGTC 2 cut(s) 1407, 1595
Van91I CCANNNNNTGG 4 cut(s) 723, 1034, 1600, 1634
VpaK11BI GGWCC 3 cut(s) 1045, 1503, 1538
XapI RAATTY 1 cut(s) 441
XbaI TCTAGA 1 cut(s) 1588
XceI RCATGY 2 cut(s) 899, 1085
XhoI CTCGAG 1 cut(s) 1449
XspI CTAG 5 cut(s) 431, 1230, 1376, 1467, 1589
ZrmI AGTACT 1 cut(s) 946
Zsp2I ATGCAT 2 cut(s) 790, 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.