Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0010958

Physical Location & Seq

Genomic Coordinates Forward (+)

27690062 .. 27693254

Gene Structure 3193 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1195800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1251 bp

ATGGGGAACTGCGGCACGAGGGAGGAATCAGCTGTGGTGACCAATGCTCAAGTTCAGCAGCTAAGCACTCTGGTGAAGAACGGTGGCGGCGAGAGGAAGCAGAATCAAAGCCGTTCGATTTCAGATCTGATCGATCCTTCGACGCCACGCAACTTCGAGGACGCGCGGAAGAATGCGGTGCTGTACACGCACGTCATCGCCTTCACGCTCTTCGAGCTCGAGACCATCACTAAGAGCTTCCGTTCCGACTACATTTTGGGGGAAGGCGGCTTCGGGACGGTGTACAAGGGCTACATTGACGAGAACGTTCGGGTCGGGTTGAAATCTCTGCCCGTTGCCGTCAAGGTCCTCAACAAGGAGGGCTACCAGGGCCACCGCGAGTGGCTGACGGAGGTCAACTTCCTTGGCCAGCTCCGTCACCCCAATCTTGTGAAATTGATTGGATACTGCTGCGAGGACGATCACAGATTGCTGGTTTACGAGTTCATGTTCCGAGGGAGCCTCGAGAATCATCTTTTCCGGAAGGCAACTGTCCCATTGTCGTGGGGGACGAGGATGATGATTGCTCTGGGAGCTGCCAAGGGGCTTGCTTTTCTTCACAATGCCGAAAGGCCTGTGATTTATCGAGATTTCAAGACTTCGAATATCTTGCTGGATTCTGATTATGCTACCAAGCTTTCGGATTTCGGGCTTGCTAAAGCTGGACCTCAAGGGGATGAGACTCATGTATCTACTCGCGTTATGGGTACCTATGGATATGCAGCACCGGAGTATGTAATGACTGGCCACCTGACTGCCAGGAGTGACGTATACAGCTTTGGAGTCGTTCTTCTTGAGCTTTTGACAGGAAGAAGATCTGTTGACAAGACGAGACCAAGCAAGGAGCAGAACCTGGTAGATTGGGCGCGGCCAAAACTGAACGACAAGAGAAAGTTGCTACAAATAATCGACCCTAGGTTGGAGAATCAGTACTCGGTCAGAGCAGCACAGAAGGCCTGCAGCTTGGCATACTACTGTCTCAGCCAAAACCCCAAAGCAAGGCCCTTAATGAGTGATGTAGTTGAAACTTTGGAACCTCTACAAAGTTGCAACGAAGCAAGTGACTCAACTGGCGGTCCATTTGCCATGACCAGAATCCCTGAGTACCGAACGCGTCACAGGTTTTCTAACAACGTAGGTCCGGGTGTTGTTTGTCGATCTCCCAACCCAAATTTATCGCCAGGAGGTCCTGCAGCGTGCAGAGTTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

417

Amino Acids

46.42

Weight (kDa)

9.12

Isoelectric Point (pI)

35.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	83 - 355	8.4e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	85 - 357	1.3e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010958)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01020
fragaria_vesca	FvH4_6g09140 FvH4_6g09140 FvH4_6g09140
malus_domestica	MD04G1180400.v1.1 MD12G1195800.v1.1
prunus_persica	Prupe.6G300300_v2.0.a1
pyrus_communis	pycom04g15950 pycom12g18200
rosa_chinensis	RchiOBHm_Chr3g0459351
rosa_laevigata	RLG00000025088
rosa_multiflora	Rmu_sc0006944.1_g000003
rosa_roxburghii	Rroxscaffold_6G00420240
rosa_rugosa	Rorug03G0035600 Rorug03G0035700
rosa_samantha	Rh3AG093700 Rh3BG096600 Rh3CG097800 Rh3DG097200
rosa_wichuraiana	Rw3G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	746
AccB1I	GGYRCC	1 cut(s)	746
AccI	GTMKAC	1 cut(s)	810
AccII	CGCG	6 cut(s)	164, 166, 378, 738, 907, 1153
AccIII	TCCGGA	1 cut(s)	519
AciI	CCGC	8 cut(s)	12, 87, 166, 176, 267, 376, 907, 1113
AclI	AACGTT	1 cut(s)	306
AclWI	GGATC	1 cut(s)	128
AcoI	YGGCCR	3 cut(s)	406, 784, 908
AcsI	RAATTY	1 cut(s)	1210
AcyI	GRCGYC	1 cut(s)	143
AfaI	GTAC	5 cut(s)	185, 284, 748, 971, 1145
AfiI	CCNNNNNNNGG	3 cut(s)	355, 958, 1038
AflIII	ACRYGT	1 cut(s)	1151
AgsI	TTSAA	3 cut(s)	322, 634, 1064
AjiI	CACGTC	1 cut(s)	193
AjnI	CCWGG	4 cut(s)	366, 797, 891, 1219
AleI	CACNNNNGTG	1 cut(s)	71
Alw21I	GWGCWC	1 cut(s)	219
Alw26I	GTCTC	4 cut(s)	215, 713, 865, 1022
AlwI	GGATC	1 cut(s)	128
AlwNI	CAGNNNCTG	1 cut(s)	892
Ama87I	CYCGRG	2 cut(s)	218, 503
Aor13HI	TCCGGA	1 cut(s)	519
AoxI	GGCC	7 cut(s)	370, 406, 611, 784, 908, 993, 1040
ApeKI	GCWGC	7 cut(s)	58, 450, 575, 761, 983, 999, 1232
ApoI	RAATTY	1 cut(s)	1210
Asp718I	GGTACC	1 cut(s)	746
AspA2I	CCTAGG	1 cut(s)	953
AspLEI	GCGC	2 cut(s)	166, 907
AspS9I	GGNCC	7 cut(s)	346, 370, 704, 1041, 1115, 1178, 1226
AsuC2I	CCSGG	1 cut(s)	1182
AsuHPI	GGTGA	3 cut(s)	49, 85, 410
AsuII	TTCGAA	1 cut(s)	641
AvaI	CYCGRG	2 cut(s)	218, 503
AvaII	GGWCC	5 cut(s)	346, 704, 1115, 1178, 1226
AvrII	CCTAGG	1 cut(s)	953
BaeI	ACNNNNGTAYC	2 cut(s)	711, 744
BalI	TGGCCA	2 cut(s)	408, 786
BanI	GGYRCC	1 cut(s)	746
BanII	GRGCYC	1 cut(s)	219
BauI	CACGAG	1 cut(s)	16
Bbv12I	GWGCWC	1 cut(s)	219
BbvI	GCAGC	7 cut(s)	70, 437, 562, 773, 995, 1011, 1244
BccI	CCATC	1 cut(s)	233
BceAI	ACGGC	2 cut(s)	96, 323
BcgI	CGANNNNNNTGC	2 cut(s)	631, 665
BciT130I	CCWGG	4 cut(s)	368, 799, 893, 1221
BciVI	GTATCC	1 cut(s)	437
BcnI	CCSGG	1 cut(s)	1182
BcoDI	GTCTC	4 cut(s)	215, 713, 865, 1022
BfaI	CTAG	1 cut(s)	954
BfmI	CTRYAG	2 cut(s)	997, 1230
BfuI	GTATCC	1 cut(s)	437
BglII	AGATCT	2 cut(s)	124, 854
BlnI	CCTAGG	1 cut(s)	953
BlpI	GCTNAGC	1 cut(s)	62
BmcAI	AGTACT	1 cut(s)	971
Bme1390I	CCNGG	5 cut(s)	368, 799, 893, 1182, 1221
Bme18I	GGWCC	5 cut(s)	346, 704, 1115, 1178, 1226
BmeT110I	CYCGRG	2 cut(s)	218, 503
BmgBI	CACGTC	1 cut(s)	193
BmgT120I	GGNCC	7 cut(s)	346, 370, 704, 1041, 1115, 1178, 1226
BmiI	GGNNCC	3 cut(s)	500, 748, 1074
BmrFI	CCNGG	5 cut(s)	368, 799, 893, 1182, 1221
BoxI	GACNNNNGTC	1 cut(s)	392
BplI	GAGNNNNNCTC	2 cut(s)	486, 518
Bpu1102I	GCTNAGC	1 cut(s)	62
Bpu14I	TTCGAA	1 cut(s)	641
BpuEI	CTTGAG	3 cut(s)	33, 693, 854
BpuMI	CCSGG	1 cut(s)	1182
Bsa29I	ATCGAT	1 cut(s)	132
BsaHI	GRCGYC	1 cut(s)	143
BsaI	GGTCTC	2 cut(s)	215, 865
BsaJI	CCNNGG	5 cut(s)	367, 403, 493, 579, 953
BsaWI	WCCGGW	2 cut(s)	519, 766
BsaXI	ACNNNNNCTCC	2 cut(s)	953, 983
Bsc4I	CCNNNNNNNGG	3 cut(s)	355, 958, 1038
Bse1I	ACTGG	2 cut(s)	787, 1114
BseAI	TCCGGA	1 cut(s)	519
BseBI	CCWGG	4 cut(s)	368, 799, 893, 1221
BseCI	ATCGAT	1 cut(s)	132
BseDI	CCNNGG	5 cut(s)	367, 403, 493, 579, 953
BseGI	GGATG	2 cut(s)	561, 721
BseLI	CCNNNNNNNGG	3 cut(s)	355, 958, 1038
BseMII	CTCAG	2 cut(s)	1033, 1131
BseNI	ACTGG	2 cut(s)	787, 1114
BseXI	GCAGC	7 cut(s)	70, 437, 562, 773, 995, 1011, 1244
Bsh1236I	CGCG	6 cut(s)	164, 166, 378, 738, 907, 1153
BshFI	GGCC	7 cut(s)	372, 408, 613, 786, 910, 995, 1042
BshNI	GGYRCC	1 cut(s)	746
BshVI	ATCGAT	1 cut(s)	132
BsiHKAI	GWGCWC	1 cut(s)	219
BsiHKCI	CYCGRG	2 cut(s)	218, 503
BsiSI	CCGG	3 cut(s)	520, 767, 1181
BslFI	GGGAC	3 cut(s)	289, 518, 562
BslI	CCNNNNNNNGG	3 cut(s)	355, 958, 1038
BsmAI	GTCTC	4 cut(s)	215, 713, 865, 1022
BsmFI	GGGAC	3 cut(s)	289, 518, 562
BsmI	GAATGC	1 cut(s)	178
BsnI	GGCC	7 cut(s)	372, 408, 613, 786, 910, 995, 1042
Bso31I	GGTCTC	2 cut(s)	215, 865
BsoBI	CYCGRG	2 cut(s)	218, 503
Bsp119I	TTCGAA	1 cut(s)	641
Bsp1286I	GDGCHC	1 cut(s)	219
Bsp13I	TCCGGA	1 cut(s)	519
Bsp1407I	TGTACA	2 cut(s)	183, 282
Bsp143I	GATC	6 cut(s)	124, 129, 133, 460, 854, 1196
Bsp1720I	GCTNAGC	1 cut(s)	62
BspACI	CCGC	8 cut(s)	12, 87, 166, 176, 267, 376, 907, 1113
BspANI	GGCC	7 cut(s)	372, 408, 613, 786, 910, 995, 1042
BspCNI	CTCAG	2 cut(s)	1032, 1132
BspDI	ATCGAT	1 cut(s)	132
BspEI	TCCGGA	1 cut(s)	519
BspFNI	CGCG	6 cut(s)	164, 166, 378, 738, 907, 1153
BspLI	GGNNCC	3 cut(s)	500, 748, 1074
BspMAI	CTGCAG	2 cut(s)	1001, 1234
BspPI	GGATC	1 cut(s)	128
BspQI	GCTCTTC	1 cut(s)	215
BspT104I	TTCGAA	1 cut(s)	641
BspT107I	GGYRCC	1 cut(s)	746
BspTNI	GGTCTC	2 cut(s)	215, 865
BsrGI	TGTACA	2 cut(s)	183, 282
BsrI	ACTGG	2 cut(s)	787, 1114
BssECI	CCNNGG	5 cut(s)	367, 403, 493, 579, 953
BssMI	GATC	6 cut(s)	124, 129, 133, 460, 854, 1196
BssNAI	GTATAC	1 cut(s)	811
BssNI	GRCGYC	1 cut(s)	143
BssSI	CACGAG	1 cut(s)	16
BssT1I	CCWWGG	3 cut(s)	403, 579, 953
Bst1107I	GTATAC	1 cut(s)	811
Bst2BI	CACGAG	1 cut(s)	16
Bst2UI	CCWGG	4 cut(s)	368, 799, 893, 1221
Bst4CI	ACNGT	4 cut(s)	83, 280, 532, 1016
Bst6I	CTCTTC	1 cut(s)	215
BstACI	GRCGYC	1 cut(s)	143
BstAUI	TGTACA	2 cut(s)	183, 282
BstBI	TTCGAA	1 cut(s)	641
BstC8I	GCNNGC	5 cut(s)	410, 588, 693, 997, 1237
BstDEI	CTNAG	4 cut(s)	62, 231, 1019, 1140
BstEII	GGTNACC	1 cut(s)	37
BstENI	CCTNNNNNAGG	1 cut(s)	353
BstF5I	GGATG	2 cut(s)	561, 721
BstFNI	CGCG	6 cut(s)	164, 166, 378, 738, 907, 1153
BstHHI	GCGC	2 cut(s)	166, 907
BstKTI	GATC	6 cut(s)	127, 132, 136, 463, 857, 1199
BstMAI	GTCTC	4 cut(s)	215, 713, 865, 1022
BstMBI	GATC	6 cut(s)	124, 129, 133, 460, 854, 1196
BstMWI	GCNNNNNNNGC	5 cut(s)	187, 214, 369, 572, 992
BstNI	CCWGG	4 cut(s)	368, 799, 893, 1221
BstPAI	GACNNNNGTC	1 cut(s)	392
BstPI	GGTNACC	1 cut(s)	37
BstSCI	CCNGG	5 cut(s)	366, 797, 891, 1180, 1219
BstSFI	CTRYAG	2 cut(s)	997, 1230
BstUI	CGCG	6 cut(s)	164, 166, 378, 738, 907, 1153
BstV1I	GCAGC	7 cut(s)	70, 437, 562, 773, 995, 1011, 1244
BstX2I	RGATCY	2 cut(s)	124, 854
BstXI	CCANNNNNNTGG	1 cut(s)	543
BstYI	RGATCY	2 cut(s)	124, 854
BstZ17I	GTATAC	1 cut(s)	811
Bsu15I	ATCGAT	1 cut(s)	132
BsuI	GTATCC	1 cut(s)	437
BsuRI	GGCC	7 cut(s)	372, 408, 613, 786, 910, 995, 1042
BsuTUI	ATCGAT	1 cut(s)	132
BtgZI	GCGATG	1 cut(s)	181
BtrI	CACGTC	1 cut(s)	193
BtsCI	GGATG	2 cut(s)	561, 721
Cac8I	GCNNGC	5 cut(s)	410, 588, 693, 997, 1237
CaiI	CAGNNNCTG	1 cut(s)	892
CfoI	GCGC	2 cut(s)	166, 907
Cfr13I	GGNCC	7 cut(s)	346, 370, 704, 1041, 1115, 1178, 1226
ClaI	ATCGAT	1 cut(s)	132
CseI	GACGC	3 cut(s)	151, 170, 1142
CsiI	ACCWGGT	1 cut(s)	891
Csp6I	GTAC	5 cut(s)	184, 283, 747, 970, 1144
CviAII	CATG	3 cut(s)	487, 725, 1126
CviQI	GTAC	5 cut(s)	184, 283, 747, 970, 1144
DdeI	CTNAG	4 cut(s)	62, 231, 1019, 1140
DpnI	GATC	6 cut(s)	126, 131, 135, 462, 856, 1198
DpnII	GATC	6 cut(s)	124, 129, 133, 460, 854, 1196
EaeI	YGGCCR	3 cut(s)	406, 784, 908
Eam1104I	CTCTTC	1 cut(s)	215
EarI	CTCTTC	1 cut(s)	215
Ecl136II	GAGCTC	1 cut(s)	217
Eco130I	CCWWGG	3 cut(s)	403, 579, 953
Eco147I	AGGCCT	2 cut(s)	613, 995
Eco24I	GRGCYC	1 cut(s)	219
Eco31I	GGTCTC	2 cut(s)	215, 865
Eco47I	GGWCC	5 cut(s)	346, 704, 1115, 1178, 1226
Eco53kI	GAGCTC	1 cut(s)	217
Eco88I	CYCGRG	2 cut(s)	218, 503
Eco91I	GGTNACC	1 cut(s)	37
EcoICRI	GAGCTC	1 cut(s)	217
EcoNI	CCTNNNNNAGG	1 cut(s)	353
EcoO109I	RGGNCCY	3 cut(s)	346, 1041, 1226
EcoO65I	GGTNACC	1 cut(s)	37
EcoRII	CCWGG	4 cut(s)	366, 797, 891, 1219
EcoT14I	CCWWGG	3 cut(s)	403, 579, 953
EcoT38I	GRGCYC	1 cut(s)	219
ErhI	CCWWGG	3 cut(s)	403, 579, 953
FaeI	CATG	3 cut(s)	490, 728, 1129
FaqI	GGGAC	3 cut(s)	289, 518, 562
FatI	CATG	3 cut(s)	486, 724, 1125
FblI	GTMKAC	1 cut(s)	810
FokI	GGATG	2 cut(s)	568, 728
FriOI	GRGCYC	1 cut(s)	219
FspBI	CTAG	1 cut(s)	954
GlaI	GCGC	2 cut(s)	165, 906
HaeIII	GGCC	7 cut(s)	372, 408, 613, 786, 910, 995, 1042
HapII	CCGG	3 cut(s)	520, 767, 1181
HgaI	GACGC	3 cut(s)	151, 170, 1142
HhaI	GCGC	2 cut(s)	166, 907
Hin1I	GRCGYC	1 cut(s)	143
Hin1II	CATG	3 cut(s)	490, 728, 1129
Hin6I	GCGC	2 cut(s)	164, 905
HinP1I	GCGC	2 cut(s)	164, 905
HincII	GTYRAC	2 cut(s)	397, 862
HindII	GTYRAC	2 cut(s)	397, 862
HindIII	AAGCTT	1 cut(s)	674
HinfI	GANTC	9 cut(s)	26, 103, 508, 656, 721, 822, 964, 1103, 1134
HpaII	CCGG	3 cut(s)	520, 767, 1181
HphI	GGTGA	3 cut(s)	49, 85, 410
Hpy166II	GTNNAC	6 cut(s)	186, 283, 397, 478, 811, 862
Hpy188I	TCNGA	7 cut(s)	124, 129, 247, 494, 661, 682, 980
Hpy188III	TCNNGA	7 cut(s)	220, 274, 505, 520, 626, 634, 833
Hpy8I	GTNNAC	6 cut(s)	186, 283, 397, 478, 811, 862
Hpy99I	CGWCG	1 cut(s)	145
HpyAV	CCTTC	5 cut(s)	147, 211, 257, 517, 985
HpyCH4III	ACNGT	4 cut(s)	83, 280, 532, 1016
HpyCH4IV	ACGT	4 cut(s)	192, 306, 807, 1173
HpyCH4V	TGCA	5 cut(s)	761, 999, 1089, 1232, 1239
HpyF10VI	GCNNNNNNNGC	5 cut(s)	187, 214, 369, 572, 992
HpyF3I	CTNAG	4 cut(s)	62, 231, 1019, 1140
HpySE526I	ACGT	4 cut(s)	192, 306, 807, 1173
Hsp92I	GRCGYC	1 cut(s)	143
Hsp92II	CATG	3 cut(s)	490, 728, 1129
HspAI	GCGC	2 cut(s)	164, 905
Kpn2I	TCCGGA	1 cut(s)	519
KpnI	GGTACC	1 cut(s)	750
Kzo9I	GATC	6 cut(s)	124, 129, 133, 460, 854, 1196
LguI	GCTCTTC	1 cut(s)	215
LmnI	GCTCC	4 cut(s)	417, 498, 572, 883
Lsp1109I	GCAGC	7 cut(s)	70, 437, 562, 773, 995, 1011, 1244
MabI	ACCWGGT	1 cut(s)	891
MaeI	CTAG	1 cut(s)	954
MaeII	ACGT	4 cut(s)	192, 306, 807, 1173
MaeIII	GTNAC	5 cut(s)	37, 416, 803, 1100, 1154
MalI	GATC	6 cut(s)	126, 131, 135, 462, 856, 1198
MboI	GATC	6 cut(s)	124, 129, 133, 460, 854, 1196
MboII	GAAGA	7 cut(s)	88, 181, 202, 587, 821, 861, 864
MflI	RGATCY	2 cut(s)	124, 854
MhlI	GDGCHC	1 cut(s)	219
MlsI	TGGCCA	2 cut(s)	408, 786
MluCI	AATT	2 cut(s)	434, 1210
MluI	ACGCGT	1 cut(s)	1151
MluNI	TGGCCA	2 cut(s)	408, 786
MlyI	GAGTC	3 cut(s)	715, 831, 1097
MmeI	TCCRAC	2 cut(s)	270, 939
Mox20I	TGGCCA	2 cut(s)	408, 786
MroI	TCCGGA	1 cut(s)	519
MscI	TGGCCA	2 cut(s)	408, 786
MseI	TTAA	1 cut(s)	1046
MslI	CAYNNNNRTG	2 cut(s)	71, 541
Msp20I	TGGCCA	2 cut(s)	408, 786
MspA1I	CMGCKG	1 cut(s)	32
MspI	CCGG	3 cut(s)	520, 767, 1181
MspR9I	CCNGG	5 cut(s)	368, 799, 893, 1182, 1221
Mva1269I	GAATGC	1 cut(s)	178
MvaI	CCWGG	4 cut(s)	368, 799, 893, 1221
MvnI	CGCG	6 cut(s)	164, 166, 378, 738, 907, 1153
MwoI	GCNNNNNNNGC	5 cut(s)	187, 214, 369, 572, 992
NciI	CCSGG	1 cut(s)	1182
NdeII	GATC	6 cut(s)	124, 129, 133, 460, 854, 1196
NlaIII	CATG	3 cut(s)	490, 728, 1129
NlaIV	GGNNCC	3 cut(s)	500, 748, 1074
NmuCI	GTSAC	5 cut(s)	37, 416, 803, 1100, 1154
NspV	TTCGAA	1 cut(s)	641
OliI	CACNNNNGTG	1 cut(s)	71
PaeR7I	CTCGAG	2 cut(s)	218, 503
PceI	AGGCCT	2 cut(s)	613, 995
PciSI	GCTCTTC	1 cut(s)	215
PcsI	WCGNNNNNNNCGW	2 cut(s)	87, 548
PctI	GAATGC	1 cut(s)	178
PfeI	GAWTC	6 cut(s)	26, 103, 508, 656, 964, 1134
PleI	GAGTC	3 cut(s)	715, 830, 1097
PpsI	GAGTC	3 cut(s)	715, 830, 1097
PpuMI	RGGWCCY	2 cut(s)	346, 1226
PshAI	GACNNNNGTC	1 cut(s)	392
Psp124BI	GAGCTC	1 cut(s)	219
Psp1406I	AACGTT	1 cut(s)	306
Psp5II	RGGWCCY	2 cut(s)	346, 1226
Psp6I	CCWGG	4 cut(s)	366, 797, 891, 1219
PspEI	GGTNACC	1 cut(s)	37
PspGI	CCWGG	4 cut(s)	366, 797, 891, 1219
PspN4I	GGNNCC	3 cut(s)	500, 748, 1074
PspPI	GGNCC	7 cut(s)	346, 370, 704, 1041, 1115, 1178, 1226
PspPPI	RGGWCCY	2 cut(s)	346, 1226
PstI	CTGCAG	2 cut(s)	1001, 1234
PstNI	CAGNNNCTG	1 cut(s)	892
PsuI	RGATCY	2 cut(s)	124, 854
PvuII	CAGCTG	1 cut(s)	32
RsaI	GTAC	5 cut(s)	185, 284, 748, 971, 1145
RsaNI	GTAC	5 cut(s)	184, 283, 747, 970, 1144
RseI	CAYNNNNRTG	2 cut(s)	71, 541
SacI	GAGCTC	1 cut(s)	219
SapI	GCTCTTC	1 cut(s)	215
SaqAI	TTAA	1 cut(s)	1046
Sau3AI	GATC	6 cut(s)	124, 129, 133, 460, 854, 1196
Sau96I	GGNCC	7 cut(s)	346, 370, 704, 1041, 1115, 1178, 1226
ScaI	AGTACT	1 cut(s)	971
SchI	GAGTC	3 cut(s)	715, 831, 1097
ScrFI	CCNGG	5 cut(s)	368, 799, 893, 1182, 1221
SduI	GDGCHC	1 cut(s)	219
SexAI	ACCWGGT	1 cut(s)	891
SfcI	CTRYAG	2 cut(s)	997, 1230
Sfr274I	CTCGAG	2 cut(s)	218, 503
SfuI	TTCGAA	1 cut(s)	641
SinI	GGWCC	5 cut(s)	346, 704, 1115, 1178, 1226
SlaI	CTCGAG	2 cut(s)	218, 503
SmiMI	CAYNNNNRTG	2 cut(s)	71, 541
SmlI	CTYRAG	5 cut(s)	48, 218, 503, 708, 833
SmoI	CTYRAG	5 cut(s)	48, 218, 503, 708, 833
Sse9I	AATT	2 cut(s)	434, 1210
SseBI	AGGCCT	2 cut(s)	613, 995
SsiI	CCGC	8 cut(s)	12, 87, 166, 176, 267, 376, 907, 1113
SspMI	CTAG	1 cut(s)	954
SstI	GAGCTC	1 cut(s)	219
StuI	AGGCCT	2 cut(s)	613, 995
StyD4I	CCNGG	5 cut(s)	366, 797, 891, 1180, 1219
StyI	CCWWGG	3 cut(s)	403, 579, 953
TaaI	ACNGT	4 cut(s)	83, 280, 532, 1016
TaiI	ACGT	4 cut(s)	195, 309, 810, 1176
TaqII	GACCGA	1 cut(s)	964
TasI	AATT	2 cut(s)	434, 1210
TatI	WGTACW	3 cut(s)	183, 282, 969
TauI	GCSGC	4 cut(s)	15, 90, 270, 910
TfiI	GAWTC	6 cut(s)	26, 103, 508, 656, 964, 1134
Tru1I	TTAA	1 cut(s)	1046
Tru9I	TTAA	1 cut(s)	1046
TseFI	GTSAC	5 cut(s)	37, 416, 803, 1100, 1154
TseI	GCWGC	7 cut(s)	58, 450, 575, 761, 983, 999, 1232
Tsp45I	GTSAC	5 cut(s)	37, 416, 803, 1100, 1154
TspDTI	ATGAA	1 cut(s)	475
TspGWI	ACGGA	3 cut(s)	230, 404, 404
VpaK11BI	GGWCC	5 cut(s)	346, 704, 1115, 1178, 1226
XagI	CCTNNNNNAGG	1 cut(s)	353
XapI	RAATTY	1 cut(s)	1210
XhoI	CTCGAG	2 cut(s)	218, 503
XmaJI	CCTAGG	1 cut(s)	953
XmiI	GTMKAC	1 cut(s)	810
XspI	CTAG	1 cut(s)	954
ZrmI	AGTACT	1 cut(s)	971

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.