Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0010958

Physical Location & Seq

Genomic Coordinates Forward (+)

20405835 .. 20408471

Gene Structure 2637 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g18200.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1254 bp

ATGGGGAACTGCGGCACGAGGGAAGAATCAGCTGTGGTGACCAATGCTCAAGCAGTTCAGCAGCTGAGCACTCTGGTGAAGAACGGCGGCGGCGAGAGGAAGCAGAATCACAGCCGTTCGATTTCAGATCTGATCGATCCTTCGACGCCACGCAACTTCGAGGACGCGCGGAAGAATGCGGTACTGTACACGCACGTCATCGCCTTCACGCTCTTCGAGCTCGAGACCATTACTAAGAGCTTCCGCTCCGACTACATTTTGGGGGAAGGCGGCTTCGGGACGGTGTACAAGGGCTACATTGACGAGAACGTTCGGGTCGGGTTGAAATCTCTGCCTGTCGCCGTCAAGGTCCTCAACAAGGAGGGCTTCCAGGGCCACCGCGAGTGGCTGACGGAGGTCAACTTCCTCGGCCAGCTCCGTCACCCCAATCTCGTGAAATTGATTGGATACTGCTGCGAAGACGATCACAGATTGCTGGTTTACGAGTTCATGTTCCGAGGGAGCCTCGAGAATCATCTTTTCCGGAAGGCAACTGTCCCATTGTTGTGGGGGACGCGGATGATGATTGCTCTAGGAGCTGCCAAGGGGCTTGCTTTTCTTCACAATGCCGAAAGGCCTGTGATTTATCGAGATTTCAAGACTTCGAATATCTTGCTGGATTCTGATTATGCTACCAAGCTTTCGGATTTCGGGCTTGCTAAAGCTGGACCTCAAGGGGATGAGACTCATGTATCTACTCGCGTTATGGGTACCTATGGATATGCAGCCCCGGAGTATGTAATGACTGGCCACCTAACTGCCCGGAGTGACGTATACAGCTTTGGAGTTGTTCTCCTTGAGCTTTTGACGGGAAGAAGATCTGTTGACAAGACGAGACCAAGCAAGGAGCAGAACCTGGTAGATTGGGCGCGGCCAAAACTGAACGACAAGAGAAAGTTGCTACAAATAATCGACCCTAGGTTGGAGAATCAGTACTCGGTCAGAGCAGCACAGAAGGCCTGCAGCTTGGCATACTACTGTCTCAGCCAAAACCCCAAAGCAAGGCCCTTAATGAGTGATGTAGTTGAAACTTTGGAACCTCTACAAAGTTGCAACGAAGCAAGCGACTCAACTGGCGGTCCATTTGCCATGACCAGAATCCCCGAGTACCGAACGCATCACAGGTTTTCTAACAACGTACGTCCGGGTGTTGTTTGTCGATCTCCCAACCCAAATTTATCGCCAGGAGGTCCTGCAGCGTGCAGAGTTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

418

Amino Acids

46.59

Weight (kDa)

9.13

Isoelectric Point (pI)

36.1

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010958)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01020
fragaria_vesca	FvH4_6g09140 FvH4_6g09140 FvH4_6g09140
malus_domestica	MD04G1180400.v1.1 MD12G1195800.v1.1
prunus_persica	Prupe.6G300300_v2.0.a1
pyrus_communis	pycom04g15950 pycom12g18200
rosa_chinensis	RchiOBHm_Chr3g0459351
rosa_laevigata	RLG00000025088
rosa_multiflora	Rmu_sc0006944.1_g000003
rosa_roxburghii	Rroxscaffold_6G00420240
rosa_rugosa	Rorug03G0035600 Rorug03G0035700
rosa_samantha	Rh3AG093700 Rh3BG096600 Rh3CG097800 Rh3DG097200
rosa_wichuraiana	Rw3G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	749
AccB1I	GGYRCC	1 cut(s)	749
AccBSI	CCGCTC	1 cut(s)	246
AccI	GTMKAC	1 cut(s)	813
AccII	CGCG	6 cut(s)	167, 169, 381, 556, 741, 910
AccIII	TCCGGA	1 cut(s)	522
AclI	AACGTT	1 cut(s)	309
AclWI	GGATC	1 cut(s)	131
AcoI	YGGCCR	3 cut(s)	409, 787, 911
AcsI	RAATTY	1 cut(s)	1213
AcyI	GRCGYC	1 cut(s)	146
AfaI	GTAC	7 cut(s)	183, 188, 287, 751, 974, 1148, 1179
AfiI	CCNNNNNNNGG	3 cut(s)	358, 961, 1041
AgsI	TTSAA	3 cut(s)	325, 637, 1067
AjiI	CACGTC	1 cut(s)	196
AjnI	CCWGG	3 cut(s)	369, 894, 1222
AleI	CACNNNNGTG	1 cut(s)	74
Alw21I	GWGCWC	2 cut(s)	71, 222
Alw26I	GTCTC	4 cut(s)	218, 716, 868, 1025
AlwI	GGATC	1 cut(s)	131
AlwNI	CAGNNNCTG	2 cut(s)	64, 895
Ama87I	CYCGRG	3 cut(s)	221, 506, 1142
Aor13HI	TCCGGA	1 cut(s)	522
AoxI	GGCC	7 cut(s)	373, 409, 614, 787, 911, 996, 1043
ApeKI	GCWGC	7 cut(s)	61, 453, 578, 764, 986, 1002, 1235
ApoI	RAATTY	1 cut(s)	1213
Asp718I	GGTACC	1 cut(s)	749
AspA2I	CCTAGG	1 cut(s)	956
AspLEI	GCGC	2 cut(s)	169, 910
AspS9I	GGNCC	6 cut(s)	349, 373, 707, 1044, 1118, 1229
AsuC2I	CCSGG	3 cut(s)	770, 802, 1185
AsuHPI	GGTGA	3 cut(s)	49, 88, 413
AsuII	TTCGAA	1 cut(s)	644
AvaI	CYCGRG	3 cut(s)	221, 506, 1142
AvaII	GGWCC	4 cut(s)	349, 707, 1118, 1229
AvrII	CCTAGG	1 cut(s)	956
BaeI	ACNNNNGTAYC	2 cut(s)	714, 747
BalI	TGGCCA	1 cut(s)	789
BanI	GGYRCC	1 cut(s)	749
BanII	GRGCYC	1 cut(s)	222
BauI	CACGAG	2 cut(s)	16, 431
BbsI	GAAGAC	1 cut(s)	465
Bbv12I	GWGCWC	2 cut(s)	71, 222
BbvI	GCAGC	7 cut(s)	73, 440, 565, 776, 998, 1014, 1247
BceAI	ACGGC	3 cut(s)	99, 100, 326
BcgI	CGANNNNNNTGC	2 cut(s)	634, 668
BciT130I	CCWGG	3 cut(s)	371, 896, 1224
BciVI	GTATCC	1 cut(s)	440
BcnI	CCSGG	3 cut(s)	770, 802, 1185
BcoDI	GTCTC	4 cut(s)	218, 716, 868, 1025
BfaI	CTAG	2 cut(s)	572, 957
BfmI	CTRYAG	2 cut(s)	1000, 1233
BfuI	GTATCC	1 cut(s)	440
BglII	AGATCT	2 cut(s)	127, 857
BlnI	CCTAGG	1 cut(s)	956
BlpI	GCTNAGC	1 cut(s)	65
BmcAI	AGTACT	1 cut(s)	974
Bme1390I	CCNGG	6 cut(s)	371, 770, 802, 896, 1185, 1224
Bme18I	GGWCC	4 cut(s)	349, 707, 1118, 1229
BmeT110I	CYCGRG	3 cut(s)	221, 506, 1142
BmgBI	CACGTC	1 cut(s)	196
BmgT120I	GGNCC	6 cut(s)	349, 373, 707, 1044, 1118, 1229
BmiI	GGNNCC	3 cut(s)	503, 751, 1077
BmrFI	CCNGG	6 cut(s)	371, 770, 802, 896, 1185, 1224
BmsI	GCATC	1 cut(s)	1165
BoxI	GACNNNNGTC	1 cut(s)	395
BpiI	GAAGAC	1 cut(s)	465
BplI	GAGNNNNNCTC	4 cut(s)	489, 521, 816, 848
Bpu1102I	GCTNAGC	1 cut(s)	65
Bpu14I	TTCGAA	1 cut(s)	644
BpuEI	CTTGAG	3 cut(s)	33, 696, 857
BpuMI	CCSGG	3 cut(s)	770, 802, 1185
Bsa29I	ATCGAT	1 cut(s)	135
BsaHI	GRCGYC	1 cut(s)	146
BsaI	GGTCTC	2 cut(s)	218, 868
BsaJI	CCNNGG	6 cut(s)	370, 406, 496, 582, 768, 956
BsaWI	WCCGGW	1 cut(s)	522
BsaXI	ACNNNNNCTCC	2 cut(s)	956, 986
Bsc4I	CCNNNNNNNGG	3 cut(s)	358, 961, 1041
Bse1I	ACTGG	2 cut(s)	790, 1117
BseAI	TCCGGA	1 cut(s)	522
BseBI	CCWGG	3 cut(s)	371, 896, 1224
BseCI	ATCGAT	1 cut(s)	135
BseDI	CCNNGG	6 cut(s)	370, 406, 496, 582, 768, 956
BseGI	GGATG	2 cut(s)	564, 724
BseLI	CCNNNNNNNGG	3 cut(s)	358, 961, 1041
BseMII	CTCAG	2 cut(s)	56, 1036
BseNI	ACTGG	2 cut(s)	790, 1117
BseXI	GCAGC	7 cut(s)	73, 440, 565, 776, 998, 1014, 1247
Bsh1236I	CGCG	6 cut(s)	167, 169, 381, 556, 741, 910
BshFI	GGCC	7 cut(s)	375, 411, 616, 789, 913, 998, 1045
BshNI	GGYRCC	1 cut(s)	749
BshVI	ATCGAT	1 cut(s)	135
BsiHKAI	GWGCWC	2 cut(s)	71, 222
BsiHKCI	CYCGRG	3 cut(s)	221, 506, 1142
BsiSI	CCGG	4 cut(s)	523, 770, 802, 1184
BsiWI	CGTACG	1 cut(s)	1177
BslFI	GGGAC	3 cut(s)	292, 521, 565
BslI	CCNNNNNNNGG	3 cut(s)	358, 961, 1041
BsmAI	GTCTC	4 cut(s)	218, 716, 868, 1025
BsmFI	GGGAC	3 cut(s)	292, 521, 565
BsmI	GAATGC	1 cut(s)	181
BsnI	GGCC	7 cut(s)	375, 411, 616, 789, 913, 998, 1045
Bso31I	GGTCTC	2 cut(s)	218, 868
BsoBI	CYCGRG	3 cut(s)	221, 506, 1142
Bsp119I	TTCGAA	1 cut(s)	644
Bsp1286I	GDGCHC	2 cut(s)	71, 222
Bsp13I	TCCGGA	1 cut(s)	522
Bsp1407I	TGTACA	2 cut(s)	186, 285
Bsp143I	GATC	6 cut(s)	127, 132, 136, 463, 857, 1199
Bsp1720I	GCTNAGC	1 cut(s)	65
BspANI	GGCC	7 cut(s)	375, 411, 616, 789, 913, 998, 1045
BspCNI	CTCAG	2 cut(s)	57, 1035
BspDI	ATCGAT	1 cut(s)	135
BspEI	TCCGGA	1 cut(s)	522
BspFNI	CGCG	6 cut(s)	167, 169, 381, 556, 741, 910
BspLI	GGNNCC	3 cut(s)	503, 751, 1077
BspMAI	CTGCAG	2 cut(s)	1004, 1237
BspPI	GGATC	1 cut(s)	131
BspQI	GCTCTTC	1 cut(s)	218
BspT104I	TTCGAA	1 cut(s)	644
BspT107I	GGYRCC	1 cut(s)	749
BspTNI	GGTCTC	2 cut(s)	218, 868
BsrBI	CCGCTC	1 cut(s)	246
BsrGI	TGTACA	2 cut(s)	186, 285
BsrI	ACTGG	2 cut(s)	790, 1117
BssECI	CCNNGG	6 cut(s)	370, 406, 496, 582, 768, 956
BssMI	GATC	6 cut(s)	127, 132, 136, 463, 857, 1199
BssNAI	GTATAC	1 cut(s)	814
BssNI	GRCGYC	1 cut(s)	146
BssSI	CACGAG	2 cut(s)	16, 431
BssT1I	CCWWGG	2 cut(s)	582, 956
Bst1107I	GTATAC	1 cut(s)	814
Bst2BI	CACGAG	2 cut(s)	16, 431
Bst2UI	CCWGG	3 cut(s)	371, 896, 1224
Bst4CI	ACNGT	4 cut(s)	186, 283, 535, 1019
Bst6I	CTCTTC	1 cut(s)	218
BstACI	GRCGYC	1 cut(s)	146
BstAUI	TGTACA	2 cut(s)	186, 285
BstBI	TTCGAA	1 cut(s)	644
BstC8I	GCNNGC	6 cut(s)	413, 591, 696, 1000, 1102, 1240
BstDEI	CTNAG	3 cut(s)	65, 234, 1022
BstEII	GGTNACC	1 cut(s)	37
BstENI	CCTNNNNNAGG	1 cut(s)	356
BstF5I	GGATG	2 cut(s)	564, 724
BstFNI	CGCG	6 cut(s)	167, 169, 381, 556, 741, 910
BstHHI	GCGC	2 cut(s)	169, 910
BstKTI	GATC	6 cut(s)	130, 135, 139, 466, 860, 1202
BstMAI	GTCTC	4 cut(s)	218, 716, 868, 1025
BstMBI	GATC	6 cut(s)	127, 132, 136, 463, 857, 1199
BstMWI	GCNNNNNNNGC	4 cut(s)	217, 372, 575, 995
BstNI	CCWGG	3 cut(s)	371, 896, 1224
BstPAI	GACNNNNGTC	1 cut(s)	395
BstPI	GGTNACC	1 cut(s)	37
BstSCI	CCNGG	6 cut(s)	369, 768, 800, 894, 1183, 1222
BstSFI	CTRYAG	2 cut(s)	1000, 1233
BstUI	CGCG	6 cut(s)	167, 169, 381, 556, 741, 910
BstV1I	GCAGC	7 cut(s)	73, 440, 565, 776, 998, 1014, 1247
BstV2I	GAAGAC	1 cut(s)	465
BstX2I	RGATCY	2 cut(s)	127, 857
BstXI	CCANNNNNNTGG	1 cut(s)	546
BstYI	RGATCY	2 cut(s)	127, 857
BstZ17I	GTATAC	1 cut(s)	814
Bsu15I	ATCGAT	1 cut(s)	135
BsuI	GTATCC	1 cut(s)	440
BsuRI	GGCC	7 cut(s)	375, 411, 616, 789, 913, 998, 1045
BsuTUI	ATCGAT	1 cut(s)	135
BtgZI	GCGATG	1 cut(s)	184
BtrI	CACGTC	1 cut(s)	196
BtsCI	GGATG	2 cut(s)	564, 724
Cac8I	GCNNGC	6 cut(s)	413, 591, 696, 1000, 1102, 1240
CaiI	CAGNNNCTG	2 cut(s)	64, 895
CfoI	GCGC	2 cut(s)	169, 910
Cfr13I	GGNCC	6 cut(s)	349, 373, 707, 1044, 1118, 1229
ClaI	ATCGAT	1 cut(s)	135
CseI	GACGC	3 cut(s)	154, 173, 562
CsiI	ACCWGGT	1 cut(s)	894
Csp6I	GTAC	7 cut(s)	182, 187, 286, 750, 973, 1147, 1178
CviAII	CATG	3 cut(s)	490, 728, 1129
CviQI	GTAC	7 cut(s)	182, 187, 286, 750, 973, 1147, 1178
DdeI	CTNAG	3 cut(s)	65, 234, 1022
DpnI	GATC	6 cut(s)	129, 134, 138, 465, 859, 1201
DpnII	GATC	6 cut(s)	127, 132, 136, 463, 857, 1199
EaeI	YGGCCR	3 cut(s)	409, 787, 911
Eam1104I	CTCTTC	1 cut(s)	218
EarI	CTCTTC	1 cut(s)	218
Ecl136II	GAGCTC	1 cut(s)	220
Eco130I	CCWWGG	2 cut(s)	582, 956
Eco147I	AGGCCT	2 cut(s)	616, 998
Eco24I	GRGCYC	1 cut(s)	222
Eco31I	GGTCTC	2 cut(s)	218, 868
Eco47I	GGWCC	4 cut(s)	349, 707, 1118, 1229
Eco53kI	GAGCTC	1 cut(s)	220
Eco88I	CYCGRG	3 cut(s)	221, 506, 1142
Eco91I	GGTNACC	1 cut(s)	37
EcoICRI	GAGCTC	1 cut(s)	220
EcoNI	CCTNNNNNAGG	1 cut(s)	356
EcoO109I	RGGNCCY	3 cut(s)	349, 1044, 1229
EcoO65I	GGTNACC	1 cut(s)	37
EcoRII	CCWGG	3 cut(s)	369, 894, 1222
EcoT14I	CCWWGG	2 cut(s)	582, 956
EcoT38I	GRGCYC	1 cut(s)	222
ErhI	CCWWGG	2 cut(s)	582, 956
FaeI	CATG	3 cut(s)	493, 731, 1132
FalI	AAGNNNNNCTT	2 cut(s)	350, 382
FaqI	GGGAC	3 cut(s)	292, 521, 565
FatI	CATG	3 cut(s)	489, 727, 1128
FblI	GTMKAC	1 cut(s)	813
FokI	GGATG	2 cut(s)	571, 731
FriOI	GRGCYC	1 cut(s)	222
FspBI	CTAG	2 cut(s)	572, 957
GlaI	GCGC	2 cut(s)	168, 909
HaeIII	GGCC	7 cut(s)	375, 411, 616, 789, 913, 998, 1045
HapII	CCGG	4 cut(s)	523, 770, 802, 1184
HgaI	GACGC	3 cut(s)	154, 173, 562
HhaI	GCGC	2 cut(s)	169, 910
Hin1I	GRCGYC	1 cut(s)	146
Hin1II	CATG	3 cut(s)	493, 731, 1132
Hin6I	GCGC	2 cut(s)	167, 908
HinP1I	GCGC	2 cut(s)	167, 908
HincII	GTYRAC	2 cut(s)	400, 865
HindII	GTYRAC	2 cut(s)	400, 865
HindIII	AAGCTT	1 cut(s)	677
HinfI	GANTC	8 cut(s)	26, 106, 511, 659, 724, 967, 1106, 1137
HpaII	CCGG	4 cut(s)	523, 770, 802, 1184
HphI	GGTGA	3 cut(s)	49, 88, 413
Hpy166II	GTNNAC	6 cut(s)	189, 286, 400, 481, 814, 865
Hpy188I	TCNGA	7 cut(s)	127, 132, 250, 497, 664, 685, 983
Hpy188III	TCNNGA	7 cut(s)	223, 277, 433, 508, 523, 629, 637
Hpy8I	GTNNAC	6 cut(s)	189, 286, 400, 481, 814, 865
Hpy99I	CGWCG	1 cut(s)	148
HpyAV	CCTTC	5 cut(s)	150, 214, 260, 520, 988
HpyCH4III	ACNGT	4 cut(s)	186, 283, 535, 1019
HpyCH4IV	ACGT	5 cut(s)	195, 309, 810, 1176, 1180
HpyCH4V	TGCA	5 cut(s)	764, 1002, 1092, 1235, 1242
HpyF10VI	GCNNNNNNNGC	4 cut(s)	217, 372, 575, 995
HpyF3I	CTNAG	3 cut(s)	65, 234, 1022
HpySE526I	ACGT	5 cut(s)	195, 309, 810, 1176, 1180
Hsp92I	GRCGYC	1 cut(s)	146
Hsp92II	CATG	3 cut(s)	493, 731, 1132
HspAI	GCGC	2 cut(s)	167, 908
Kpn2I	TCCGGA	1 cut(s)	522
KpnI	GGTACC	1 cut(s)	753
Kzo9I	GATC	6 cut(s)	127, 132, 136, 463, 857, 1199
LguI	GCTCTTC	1 cut(s)	218
LmnI	GCTCC	5 cut(s)	251, 420, 501, 575, 886
Lsp1109I	GCAGC	7 cut(s)	73, 440, 565, 776, 998, 1014, 1247
LweI	GCATC	1 cut(s)	1165
MabI	ACCWGGT	1 cut(s)	894
MaeI	CTAG	2 cut(s)	572, 957
MaeII	ACGT	5 cut(s)	195, 309, 810, 1176, 1180
MaeIII	GTNAC	3 cut(s)	37, 419, 806
MalI	GATC	6 cut(s)	129, 134, 138, 465, 859, 1201
MbiI	CCGCTC	1 cut(s)	246
MboI	GATC	6 cut(s)	127, 132, 136, 463, 857, 1199
MboII	GAAGA	8 cut(s)	35, 91, 184, 205, 470, 590, 864, 867
MflI	RGATCY	2 cut(s)	127, 857
MhlI	GDGCHC	2 cut(s)	71, 222
MlsI	TGGCCA	1 cut(s)	789
MluCI	AATT	2 cut(s)	437, 1213
MluNI	TGGCCA	1 cut(s)	789
MlyI	GAGTC	2 cut(s)	718, 1100
MmeI	TCCRAC	2 cut(s)	273, 942
Mox20I	TGGCCA	1 cut(s)	789
MroI	TCCGGA	1 cut(s)	522
MscI	TGGCCA	1 cut(s)	789
MseI	TTAA	1 cut(s)	1049
MslI	CAYNNNNRTG	2 cut(s)	74, 544
Msp20I	TGGCCA	1 cut(s)	789
MspA1I	CMGCKG	2 cut(s)	32, 64
MspI	CCGG	4 cut(s)	523, 770, 802, 1184
MspR9I	CCNGG	6 cut(s)	371, 770, 802, 896, 1185, 1224
Mva1269I	GAATGC	1 cut(s)	181
MvaI	CCWGG	3 cut(s)	371, 896, 1224
MvnI	CGCG	6 cut(s)	167, 169, 381, 556, 741, 910
MwoI	GCNNNNNNNGC	4 cut(s)	217, 372, 575, 995
NciI	CCSGG	3 cut(s)	770, 802, 1185
NdeII	GATC	6 cut(s)	127, 132, 136, 463, 857, 1199
NlaIII	CATG	3 cut(s)	493, 731, 1132
NlaIV	GGNNCC	3 cut(s)	503, 751, 1077
NmeAIII	GCCGAG	1 cut(s)	387
NmuCI	GTSAC	3 cut(s)	37, 419, 806
NspV	TTCGAA	1 cut(s)	644
OliI	CACNNNNGTG	1 cut(s)	74
PaeR7I	CTCGAG	2 cut(s)	221, 506
PceI	AGGCCT	2 cut(s)	616, 998
PciSI	GCTCTTC	1 cut(s)	218
PcsI	WCGNNNNNNNCGW	2 cut(s)	90, 1101
PctI	GAATGC	1 cut(s)	181
PfeI	GAWTC	6 cut(s)	26, 106, 511, 659, 967, 1137
Pfl23II	CGTACG	1 cut(s)	1177
PleI	GAGTC	2 cut(s)	718, 1100
PpsI	GAGTC	2 cut(s)	718, 1100
PpuMI	RGGWCCY	2 cut(s)	349, 1229
PshAI	GACNNNNGTC	1 cut(s)	395
Psp124BI	GAGCTC	1 cut(s)	222
Psp1406I	AACGTT	1 cut(s)	309
Psp5II	RGGWCCY	2 cut(s)	349, 1229
Psp6I	CCWGG	3 cut(s)	369, 894, 1222
PspEI	GGTNACC	1 cut(s)	37
PspGI	CCWGG	3 cut(s)	369, 894, 1222
PspLI	CGTACG	1 cut(s)	1177
PspN4I	GGNNCC	3 cut(s)	503, 751, 1077
PspPI	GGNCC	6 cut(s)	349, 373, 707, 1044, 1118, 1229
PspPPI	RGGWCCY	2 cut(s)	349, 1229
PstI	CTGCAG	2 cut(s)	1004, 1237
PstNI	CAGNNNCTG	2 cut(s)	64, 895
PsuI	RGATCY	2 cut(s)	127, 857
PvuII	CAGCTG	2 cut(s)	32, 64
RsaI	GTAC	7 cut(s)	183, 188, 287, 751, 974, 1148, 1179
RsaNI	GTAC	7 cut(s)	182, 187, 286, 750, 973, 1147, 1178
RseI	CAYNNNNRTG	2 cut(s)	74, 544
SacI	GAGCTC	1 cut(s)	222
SapI	GCTCTTC	1 cut(s)	218
SaqAI	TTAA	1 cut(s)	1049
Sau3AI	GATC	6 cut(s)	127, 132, 136, 463, 857, 1199
Sau96I	GGNCC	6 cut(s)	349, 373, 707, 1044, 1118, 1229
ScaI	AGTACT	1 cut(s)	974
SchI	GAGTC	2 cut(s)	718, 1100
ScrFI	CCNGG	6 cut(s)	371, 770, 802, 896, 1185, 1224
SduI	GDGCHC	2 cut(s)	71, 222
SexAI	ACCWGGT	1 cut(s)	894
SfaNI	GCATC	1 cut(s)	1165
SfcI	CTRYAG	2 cut(s)	1000, 1233
Sfr274I	CTCGAG	2 cut(s)	221, 506
SfuI	TTCGAA	1 cut(s)	644
SinI	GGWCC	4 cut(s)	349, 707, 1118, 1229
SlaI	CTCGAG	2 cut(s)	221, 506
SmiMI	CAYNNNNRTG	2 cut(s)	74, 544
SmlI	CTYRAG	5 cut(s)	48, 221, 506, 711, 836
SmoI	CTYRAG	5 cut(s)	48, 221, 506, 711, 836
Sse9I	AATT	2 cut(s)	437, 1213
SseBI	AGGCCT	2 cut(s)	616, 998
SspMI	CTAG	2 cut(s)	572, 957
SstI	GAGCTC	1 cut(s)	222
StuI	AGGCCT	2 cut(s)	616, 998
StyD4I	CCNGG	6 cut(s)	369, 768, 800, 894, 1183, 1222
StyI	CCWWGG	2 cut(s)	582, 956
TaaI	ACNGT	4 cut(s)	186, 283, 535, 1019
TaiI	ACGT	5 cut(s)	198, 312, 813, 1179, 1183
TaqII	GACCGA	1 cut(s)	967
TasI	AATT	2 cut(s)	437, 1213
TatI	WGTACW	3 cut(s)	186, 285, 972
TauI	GCSGC	5 cut(s)	15, 90, 93, 273, 913
TfiI	GAWTC	6 cut(s)	26, 106, 511, 659, 967, 1137
Tru1I	TTAA	1 cut(s)	1049
Tru9I	TTAA	1 cut(s)	1049
TseFI	GTSAC	3 cut(s)	37, 419, 806
TseI	GCWGC	7 cut(s)	61, 453, 578, 764, 986, 1002, 1235
Tsp45I	GTSAC	3 cut(s)	37, 419, 806
TspDTI	ATGAA	1 cut(s)	478
TspGWI	ACGGA	2 cut(s)	407, 407
VpaK11BI	GGWCC	4 cut(s)	349, 707, 1118, 1229
XagI	CCTNNNNNAGG	1 cut(s)	356
XapI	RAATTY	1 cut(s)	1213
XhoI	CTCGAG	2 cut(s)	221, 506
XmaJI	CCTAGG	1 cut(s)	956
XmiI	GTMKAC	1 cut(s)	813
XspI	CTAG	2 cut(s)	572, 957
ZrmI	AGTACT	1 cut(s)	974

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.