Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011574

Physical Location & Seq

Genomic Coordinates Reverse (-)

28126770 .. 28130369

Gene Structure 3600 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1200600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1887 bp

ATGGCAATTGCAGCTTCTAAGTGCCTCTTCCTTTTCTTCCTTTCTCTCCTCTCTTGTTGTTCATCAGGAACTCTGGTGGGGTTTTCATATCATGCAAGTGGAAACACTACAACTTCATCACCAGTCAGAACAATATCATTCCTGAAGCAGAACAAGATCAACCTGTCTCAGATTCGAGTCTTTGTCGAAGATCCTAGGGTTTTAAGCACTCTGTCCAAAACCGGCGTAGCTGTTGATCTCTACTTGAATGAGAGGCAGGTTGAAAATCTGATGAGCTCCAAATCTTCCTCAAAATCATGGCTCAAAACCCACACAATGCCCTTCTTCTCAAAAGCAGATATCAAAAGCATCATAGCAATTGGTGGCAGTGATCATACAAGAAAATCTAAGCTACCTAGGGTTTTATCCAACCTCAAAACCCTACACAAGGTCCTCACTAGTCTTCCTTTCGACCGAAGAATTAAGGTCTCGGTAGCATTTTCTCTGCCATTTCTTGAGAATTTGAGAAGAGGAAATGGAAAAGACCTGCACAGGATCTGTAGTTTTATTAATGAAGTGAGATCTTCTGTCATAGTAGAAGCCAATGTCGACGGAGAATTGAACATGGGAGATAGGTTTGTTCAGTCAGTGATCGAAAAAGCCGGCCTTGCTCATTCTGTTCTTCCTTGTAATGCTGTCCCCATGGTTTTGACAATCAAGAGTCCAGCAGCTCCCAGCGCAACTGAAGTGGCTGAGTTCACAGATAAGGTTTCAAAAGCTTTAGAAAGCGAGACTCGGATTACAAGTAGGCTAGTAGGGTTATACGCAGAAGTAGATTCCATGGAAGATTTTGCGCAAAAAGAGCTCAAGAGGGAAGAGGAACAGATCCTTCCTTCTTCTAGAAGAGAAATCCTAAGCGATCCCCACCCGAAAACAACTTCACATGATGTATATGGCTCACCAACAATCTTCCCTACAAATCCAACAACACCAGCAGTACCTACAACTTCTCCATACACCTCTTCCCCAACCACTGTCACTGTCCCGGCTACAAACCCGGTAACAGTAACTCCGGTTAACCCCGCTGCGCCTGTGGAAGTTCCCTCCACCACACCCATCATTCTTCCCCCCTCAAACCCTGTGAGTTCATCAGTGCCAGTCACCAATCCAGCCACAACTCCAACAATAACTAACCCTGCGACAACTTACCCGCCGCCAACTGGAGGTGTTCCCTCCACAACAACACCTGTCACGAATCCGGTCACACCTCCTTCAACAACTAACGCTCCTGCAGTTCCAGGGCAGAGTTGGTGTGTGGCAAAGAGTGGAGCACCACAAGCAGCACTTCAGGCAGCTTTGGATTATGCATGTGGAATGGGAAGCGCAGATTGCTCACAGATCCAGCAGGGTGGGAGCTGCTACAATCCAATTTCTCTGCAAAATCATGCCTCCTTTGCCTTCAACAGCTACTATCAGAAGAATCCTGTGTCAACTAGCTGTGACTTTGGAGGGGTTGCCACAATAGTTAGTGCTAATCCAAGCACTGGTTCCTGCATTTATGCATCATCATCATCAACAACACCAACTCCGAATCCGGCATCAACAAATCCACCACCAACATCAACAATTCCACCACCAACGTCAACAATTCCACCACCAACGTCAACAACTCCGCCAGCAATCACAACATTTCCACCACCTGGGGAAGGTGTATCAGGCTCTGGTACTCCGCCTTCAGTTTTGAACTCAAGCAACCCGGCTAATTCAGGAACCATGCCAGAACTTGGGCCCTACACCCCTCCCGGTTTCAACACAACAACATCCACTTCAGCTAGCTTGCAACCGTCTTTTGGTTGCATCTTTTTCGTAGCAACCTTAGTTACAAGAATTTTTTTTCTTAACATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016787 GO:0016798 GO:0030246 GO:0030247 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_17 X8

Protein Analysis

629

Amino Acids

66.15

Weight (kDa)

8.51

Isoelectric Point (pI)

51.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_17	PF00332	27 - 167	1.1e-06	Glycosyl hydrolases family 17
X8	PF07983	429 - 500	4.8e-21	X8 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011574)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g08400 FvH4_6g08400 FvH4_6g08400 FvH4_6g08400
malus_domestica	MD04G1187200.v1.1 MD12G1200600.v1.1
prunus_persica	Prupe.6G305700_v2.0.a1
pyrus_communis	pycom04g16530 pycom12g18710
rosa_chinensis	RchiOBHm_Chr3g0458371
rosa_laevigata	RLG00000025165
rosa_multiflora	Rmu_sc0003967.1_g000004
rosa_roxburghii	Rroxscaffold_6G00421140
rosa_rugosa	Rorug03G0026100 Rorug03G0026200
rosa_samantha	Rh3AG087100 Rh3BG089800 Rh3CG090200 Rh3DG090800
rosa_wichuraiana	Rw3G007390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	834
Acc36I	ACCTGC	2 cut(s)	247, 534
AccB7I	CCANNNNNTGG	3 cut(s)	1523, 1679, 1763
AccI	GTMKAC	1 cut(s)	588
AciI	CCGC	5 cut(s)	1062, 1190, 1193, 1652, 1709
AclWI	GGATC	5 cut(s)	185, 542, 859, 893, 1372
AcsI	RAATTY	2 cut(s)	499, 1866
AcuI	CTGAAG	5 cut(s)	164, 744, 1310, 1698, 1791
AfaI	GTAC	2 cut(s)	978, 1705
AfiI	CCNNNNNNNGG	8 cut(s)	427, 1199, 1202, 1523, 1679, 1685, 1763, 1829
AflIII	ACRYGT	1 cut(s)	1881
AgsI	TTSAA	8 cut(s)	247, 263, 601, 753, 1254, 1441, 1723, 1789
AhlI	ACTAGT	1 cut(s)	437
AjnI	CCWGG	2 cut(s)	1276, 1678
AjuI	GAANNNNNNNTTGG	2 cut(s)	1510, 1542
Alw21I	GWGCWC	3 cut(s)	278, 846, 1312
Alw26I	GTCTC	3 cut(s)	171, 472, 764
AlwI	GGATC	5 cut(s)	185, 542, 859, 893, 1372
AlwNI	CAGNNNCTG	2 cut(s)	537, 1700
AoxI	GGCC	2 cut(s)	643, 1766
ApaI	GGGCCC	1 cut(s)	1770
ApeKI	GCWGC	6 cut(s)	11, 707, 1064, 1319, 1331, 1395
ApoI	RAATTY	2 cut(s)	499, 1866
AseI	ATTAAT	1 cut(s)	549
AspA2I	CCTAGG	2 cut(s)	194, 395
AspLEI	GCGC	4 cut(s)	719, 835, 1069, 1364
AspS9I	GGNCC	3 cut(s)	430, 1766, 1767
AsuC2I	CCSGG	4 cut(s)	1025, 1037, 1736, 1782
AsuHPI	GGTGA	3 cut(s)	111, 930, 1132
AsuNHI	GCTAGC	1 cut(s)	1811
AvaII	GGWCC	1 cut(s)	430
AvrII	CCTAGG	2 cut(s)	194, 395
BaeGI	GKGCMC	1 cut(s)	1770
BanII	GRGCYC	3 cut(s)	278, 846, 1770
BarI	GAAGNNNNNNTAC	2 cut(s)	1696, 1728
BbsI	GAAGAC	1 cut(s)	434
Bbv12I	GWGCWC	3 cut(s)	278, 846, 1312
BbvI	GCAGC	6 cut(s)	23, 719, 1051, 1331, 1343, 1382
BccI	CCATC	1 cut(s)	1103
BcgI	CGANNNNNNTGC	2 cut(s)	1825, 1859
BciT130I	CCWGG	2 cut(s)	1278, 1680
BclI	TGATCA	1 cut(s)	370
BcnI	CCSGG	4 cut(s)	1025, 1037, 1736, 1782
BcoDI	GTCTC	3 cut(s)	171, 472, 764
BcuI	ACTAGT	1 cut(s)	437
BfaI	CTAG	7 cut(s)	195, 396, 438, 791, 879, 1473, 1812
BfmI	CTRYAG	2 cut(s)	538, 1269
BfuAI	ACCTGC	2 cut(s)	247, 534
BglII	AGATCT	1 cut(s)	560
BisI	GCNGC	7 cut(s)	12, 708, 1065, 1193, 1320, 1332, 1396
BlnI	CCTAGG	2 cut(s)	194, 395
BlsI	GCNGC	7 cut(s)	13, 709, 1066, 1194, 1321, 1333, 1397
Bme1390I	CCNGG	6 cut(s)	1025, 1037, 1278, 1680, 1736, 1782
Bme18I	GGWCC	1 cut(s)	430
BmgT120I	GGNCC	3 cut(s)	430, 1766, 1767
BmiI	GGNNCC	3 cut(s)	1528, 1750, 1768
BmrFI	CCNGG	6 cut(s)	1025, 1037, 1278, 1680, 1736, 1782
BmsI	GCATC	4 cut(s)	357, 1550, 1586, 1845
BmtI	GCTAGC	1 cut(s)	1815
BpiI	GAAGAC	1 cut(s)	434
BpmI	CTGGAG	1 cut(s)	1221
Bpu10I	CCTNAGC	1 cut(s)	893
BpuEI	CTTGAG	3 cut(s)	515, 830, 1711
BpuMI	CCSGG	4 cut(s)	1025, 1037, 1736, 1782
BsaI	GGTCTC	1 cut(s)	472
BsaJI	CCNNGG	6 cut(s)	194, 395, 681, 819, 1277, 1679
BsaWI	WCCGGW	2 cut(s)	1051, 1237
BsaXI	ACNNNNNCTCC	8 cut(s)	973, 1003, 1033, 1063, 1249, 1279, 1549, 1579
Bsc4I	CCNNNNNNNGG	8 cut(s)	427, 1199, 1202, 1523, 1679, 1685, 1763, 1829
Bse118I	RCCGGY	2 cut(s)	221, 641
Bse1I	ACTGG	4 cut(s)	122, 1136, 1204, 1528
BseBI	CCWGG	2 cut(s)	1278, 1680
BseDI	CCNNGG	6 cut(s)	194, 395, 681, 819, 1277, 1679
BseGI	GGATG	1 cut(s)	1799
BseLI	CCNNNNNNNGG	8 cut(s)	427, 1199, 1202, 1523, 1679, 1685, 1763, 1829
BseMII	CTCAG	2 cut(s)	182, 723
BseNI	ACTGG	4 cut(s)	122, 1136, 1204, 1528
BseRI	GAGGAG	1 cut(s)	38
BseSI	GKGCMC	1 cut(s)	1770
BseXI	GCAGC	6 cut(s)	23, 719, 1051, 1331, 1343, 1382
BseYI	CCCAGC	1 cut(s)	713
BsgI	GTGCAG	1 cut(s)	512
Bsh1285I	CGRYCG	1 cut(s)	454
BshFI	GGCC	2 cut(s)	645, 1768
BsiEI	CGRYCG	1 cut(s)	454
BsiHKAI	GWGCWC	3 cut(s)	278, 846, 1312
BsiSI	CCGG	9 cut(s)	222, 642, 1025, 1037, 1052, 1238, 1574, 1736, 1782
BslFI	GGGAC	2 cut(s)	662, 1007
BslI	CCNNNNNNNGG	8 cut(s)	427, 1199, 1202, 1523, 1679, 1685, 1763, 1829
BsmAI	GTCTC	3 cut(s)	171, 472, 764
BsmFI	GGGAC	2 cut(s)	662, 1007
BsnI	GGCC	2 cut(s)	645, 1768
Bso31I	GGTCTC	1 cut(s)	472
Bsp120I	GGGCCC	1 cut(s)	1766
Bsp1286I	GDGCHC	4 cut(s)	278, 846, 1312, 1770
Bsp19I	CCATGG	2 cut(s)	681, 819
BspACI	CCGC	5 cut(s)	1062, 1190, 1193, 1652, 1709
BspANI	GGCC	2 cut(s)	645, 1768
BspCNI	CTCAG	2 cut(s)	181, 724
BspLI	GGNNCC	3 cut(s)	1528, 1750, 1768
BspMAI	CTGCAG	1 cut(s)	1273
BspMI	ACCTGC	2 cut(s)	247, 534
BspOI	GCTAGC	1 cut(s)	1815
BspPI	GGATC	5 cut(s)	185, 542, 859, 893, 1372
BspTNI	GGTCTC	1 cut(s)	472
BsrFI	RCCGGY	2 cut(s)	221, 641
BsrI	ACTGG	4 cut(s)	122, 1136, 1204, 1528
BssAI	RCCGGY	2 cut(s)	221, 641
BssECI	CCNNGG	6 cut(s)	194, 395, 681, 819, 1277, 1679
BssT1I	CCWWGG	4 cut(s)	194, 395, 681, 819
Bst2UI	CCWGG	2 cut(s)	1278, 1680
Bst4CI	ACNGT	4 cut(s)	1015, 1021, 1045, 1824
Bst6I	CTCTTC	5 cut(s)	32, 502, 849, 877, 1006
BstC8I	GCNNGC	4 cut(s)	643, 1656, 1813, 1817
BstDEI	CTNAG	6 cut(s)	18, 168, 387, 732, 893, 1855
BstDSI	CCRYGG	2 cut(s)	681, 819
BstENI	CCTNNNNNAGG	2 cut(s)	425, 1683
BstF5I	GGATG	1 cut(s)	1799
BstHHI	GCGC	4 cut(s)	719, 835, 1069, 1364
BstMAI	GTCTC	3 cut(s)	171, 472, 764
BstMCI	CGRYCG	1 cut(s)	454
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 647, 716, 841, 1316, 1328, 1368, 1433
BstNI	CCWGG	2 cut(s)	1278, 1680
BstNSI	RCATGY	2 cut(s)	1350, 1885
BstSCI	CCNGG	6 cut(s)	1023, 1035, 1276, 1678, 1734, 1780
BstSFI	CTRYAG	2 cut(s)	538, 1269
BstSLI	GKGCMC	1 cut(s)	1770
BstV1I	GCAGC	6 cut(s)	23, 719, 1051, 1331, 1343, 1382
BstV2I	GAAGAC	1 cut(s)	434
BstX2I	RGATCY	5 cut(s)	190, 534, 560, 864, 1377
BstXI	CCANNNNNNTGG	1 cut(s)	1388
BstYI	RGATCY	5 cut(s)	190, 534, 560, 864, 1377
BsuRI	GGCC	2 cut(s)	645, 1768
BtgI	CCRYGG	2 cut(s)	681, 819
BtsCI	GGATG	1 cut(s)	1799
BtsI	GCAGTG	1 cut(s)	373
BtsIMutI	CAGTG	6 cut(s)	373, 633, 1011, 1017, 1137, 1521
BveI	ACCTGC	2 cut(s)	247, 534
Cac8I	GCNNGC	4 cut(s)	643, 1656, 1813, 1817
CaiI	CAGNNNCTG	2 cut(s)	537, 1700
CfoI	GCGC	4 cut(s)	719, 835, 1069, 1364
Cfr10I	RCCGGY	2 cut(s)	221, 641
Cfr13I	GGNCC	3 cut(s)	430, 1766, 1767
Csp6I	GTAC	2 cut(s)	977, 1704
CspCI	CAANNNNNGTGG	2 cut(s)	708, 743
CviQI	GTAC	2 cut(s)	977, 1704
DdeI	CTNAG	6 cut(s)	18, 168, 387, 732, 893, 1855
Eam1104I	CTCTTC	5 cut(s)	32, 502, 849, 877, 1006
EarI	CTCTTC	5 cut(s)	32, 502, 849, 877, 1006
EciI	GGCGGA	2 cut(s)	1641, 1698
Ecl136II	GAGCTC	2 cut(s)	276, 844
Eco130I	CCWWGG	4 cut(s)	194, 395, 681, 819
Eco24I	GRGCYC	3 cut(s)	278, 846, 1770
Eco31I	GGTCTC	1 cut(s)	472
Eco32I	GATATC	1 cut(s)	340
Eco47I	GGWCC	1 cut(s)	430
Eco53kI	GAGCTC	2 cut(s)	276, 844
Eco57I	CTGAAG	5 cut(s)	164, 744, 1310, 1698, 1791
EcoICRI	GAGCTC	2 cut(s)	276, 844
EcoNI	CCTNNNNNAGG	2 cut(s)	425, 1683
EcoO109I	RGGNCCY	2 cut(s)	430, 1767
EcoRII	CCWGG	2 cut(s)	1276, 1678
EcoRV	GATATC	1 cut(s)	340
EcoT14I	CCWWGG	4 cut(s)	194, 395, 681, 819
EcoT22I	ATGCAT	2 cut(s)	1348, 1543
EcoT38I	GRGCYC	3 cut(s)	278, 846, 1770
ErhI	CCWWGG	4 cut(s)	194, 395, 681, 819
FalI	AAGNNNNNCTT	6 cut(s)	11, 43, 630, 662, 1308, 1340
FaqI	GGGAC	2 cut(s)	662, 1007
FauI	CCCGC	2 cut(s)	1069, 1197
FbaI	TGATCA	1 cut(s)	370
FblI	GTMKAC	1 cut(s)	588
Fnu4HI	GCNGC	7 cut(s)	12, 708, 1065, 1193, 1320, 1332, 1396
FokI	GGATG	1 cut(s)	1786
FriOI	GRGCYC	3 cut(s)	278, 846, 1770
Fsp4HI	GCNGC	7 cut(s)	12, 708, 1065, 1193, 1320, 1332, 1396
FspBI	CTAG	7 cut(s)	195, 396, 438, 791, 879, 1473, 1812
FspI	TGCGCA	1 cut(s)	834
GlaI	GCGC	4 cut(s)	718, 834, 1068, 1363
GluI	GCNGC	7 cut(s)	12, 708, 1065, 1193, 1320, 1332, 1396
GsaI	CCCAGC	1 cut(s)	717
GsuI	CTGGAG	1 cut(s)	1221
HaeIII	GGCC	2 cut(s)	645, 1768
HapII	CCGG	9 cut(s)	222, 642, 1025, 1037, 1052, 1238, 1574, 1736, 1782
HhaI	GCGC	4 cut(s)	719, 835, 1069, 1364
Hin6I	GCGC	4 cut(s)	717, 833, 1067, 1362
HinP1I	GCGC	4 cut(s)	717, 833, 1067, 1362
HincII	GTYRAC	5 cut(s)	589, 1057, 1470, 1623, 1644
HindII	GTYRAC	5 cut(s)	589, 1057, 1470, 1623, 1644
HindIII	AAGCTT	1 cut(s)	756
HinfI	GANTC	8 cut(s)	172, 177, 700, 772, 815, 1234, 1459, 1570
HpaI	GTTAAC	1 cut(s)	1057
HpaII	CCGG	9 cut(s)	222, 642, 1025, 1037, 1052, 1238, 1574, 1736, 1782
HphI	GGTGA	3 cut(s)	111, 930, 1132
Hpy166II	GTNNAC	6 cut(s)	589, 738, 1057, 1470, 1623, 1644
Hpy188I	TCNGA	6 cut(s)	128, 171, 270, 777, 1455, 1569
Hpy188III	TCNNGA	8 cut(s)	66, 142, 494, 697, 847, 879, 1231, 1746
Hpy8I	GTNNAC	6 cut(s)	589, 738, 1057, 1470, 1623, 1644
Hpy99I	CGWCG	1 cut(s)	593
HpyAV	CCTTC	7 cut(s)	331, 878, 882, 1260, 1447, 1679, 1722
HpyCH4III	ACNGT	4 cut(s)	1015, 1021, 1045, 1824
HpyCH4IV	ACGT	2 cut(s)	1619, 1640
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 647, 716, 841, 1316, 1328, 1368, 1433
HpyF3I	CTNAG	6 cut(s)	18, 168, 387, 732, 893, 1855
HpySE526I	ACGT	2 cut(s)	1619, 1640
HspAI	GCGC	4 cut(s)	717, 833, 1067, 1362
KroI	GCCGGC	1 cut(s)	641
KroNI	GCCGGC	1 cut(s)	643
Ksp22I	TGATCA	1 cut(s)	370
KspAI	GTTAAC	1 cut(s)	1057
LmnI	GCTCC	5 cut(s)	281, 715, 1270, 1307, 1392
Lsp1109I	GCAGC	6 cut(s)	23, 719, 1051, 1331, 1343, 1382
LweI	GCATC	4 cut(s)	357, 1550, 1586, 1845
MaeI	CTAG	7 cut(s)	195, 396, 438, 791, 879, 1473, 1812
MaeII	ACGT	2 cut(s)	1619, 1640
MaeIII	GTNAC	8 cut(s)	1015, 1039, 1045, 1138, 1228, 1240, 1478, 1858
MfeI	CAATTG	2 cut(s)	6, 357
MflI	RGATCY	5 cut(s)	190, 534, 560, 864, 1377
MhlI	GDGCHC	4 cut(s)	278, 846, 1312, 1770
MlyI	GAGTC	3 cut(s)	186, 709, 766
MmeI	TCCRAC	3 cut(s)	432, 986, 1184
Mph1103I	ATGCAT	2 cut(s)	1348, 1543
MroNI	GCCGGC	1 cut(s)	641
MseI	TTAA	5 cut(s)	203, 462, 549, 1056, 1878
MslI	CAYNNNNRTG	1 cut(s)	96
MspA1I	CMGCKG	1 cut(s)	1064
MspI	CCGG	9 cut(s)	222, 642, 1025, 1037, 1052, 1238, 1574, 1736, 1782
MspR9I	CCNGG	6 cut(s)	1025, 1037, 1278, 1680, 1736, 1782
MunI	CAATTG	2 cut(s)	6, 357
MvaI	CCWGG	2 cut(s)	1278, 1680
MwoI	GCNNNNNNNGC	8 cut(s)	11, 647, 716, 841, 1316, 1328, 1368, 1433
NaeI	GCCGGC	1 cut(s)	643
NciI	CCSGG	4 cut(s)	1025, 1037, 1736, 1782
NcoI	CCATGG	2 cut(s)	681, 819
NgoMIV	GCCGGC	1 cut(s)	641
NheI	GCTAGC	1 cut(s)	1811
NlaIV	GGNNCC	3 cut(s)	1528, 1750, 1768
NmuCI	GTSAC	5 cut(s)	1015, 1138, 1228, 1240, 1478
NsbI	TGCGCA	1 cut(s)	834
NsiI	ATGCAT	2 cut(s)	1348, 1543
NspI	RCATGY	2 cut(s)	1350, 1885
PciI	ACATGT	1 cut(s)	1881
PdiI	GCCGGC	1 cut(s)	643
PfeI	GAWTC	5 cut(s)	172, 815, 1234, 1459, 1570
PflMI	CCANNNNNTGG	3 cut(s)	1523, 1679, 1763
PkrI	GCNGC	7 cut(s)	13, 709, 1066, 1194, 1321, 1333, 1397
PleI	GAGTC	3 cut(s)	185, 708, 766
PpsI	GAGTC	3 cut(s)	185, 708, 766
PpuMI	RGGWCCY	1 cut(s)	430
PscI	ACATGT	1 cut(s)	1881
PshBI	ATTAAT	1 cut(s)	549
Psp124BI	GAGCTC	2 cut(s)	278, 846
Psp5II	RGGWCCY	1 cut(s)	430
Psp6I	CCWGG	2 cut(s)	1276, 1678
PspFI	CCCAGC	1 cut(s)	713
PspGI	CCWGG	2 cut(s)	1276, 1678
PspN4I	GGNNCC	3 cut(s)	1528, 1750, 1768
PspOMI	GGGCCC	1 cut(s)	1766
PspPI	GGNCC	3 cut(s)	430, 1766, 1767
PspPPI	RGGWCCY	1 cut(s)	430
PstI	CTGCAG	1 cut(s)	1273
PstNI	CAGNNNCTG	2 cut(s)	537, 1700
PsuI	RGATCY	5 cut(s)	190, 534, 560, 864, 1377
RsaI	GTAC	2 cut(s)	978, 1705
RsaNI	GTAC	2 cut(s)	977, 1704
RseI	CAYNNNNRTG	1 cut(s)	96
SacI	GAGCTC	2 cut(s)	278, 846
SalI	GTCGAC	1 cut(s)	587
SaqAI	TTAA	5 cut(s)	203, 462, 549, 1056, 1878
SatI	GCNGC	7 cut(s)	12, 708, 1065, 1193, 1320, 1332, 1396
Sau96I	GGNCC	3 cut(s)	430, 1766, 1767
SchI	GAGTC	3 cut(s)	186, 709, 766
ScrFI	CCNGG	6 cut(s)	1025, 1037, 1278, 1680, 1736, 1782
SduI	GDGCHC	4 cut(s)	278, 846, 1312, 1770
SfaNI	GCATC	4 cut(s)	357, 1550, 1586, 1845
SfcI	CTRYAG	2 cut(s)	538, 1269
SinI	GGWCC	1 cut(s)	430
SmiMI	CAYNNNNRTG	1 cut(s)	96
SmlI	CTYRAG	3 cut(s)	494, 845, 1726
SmoI	CTYRAG	3 cut(s)	494, 845, 1726
SpeI	ACTAGT	1 cut(s)	437
SsiI	CCGC	5 cut(s)	1062, 1190, 1193, 1652, 1709
SspMI	CTAG	7 cut(s)	195, 396, 438, 791, 879, 1473, 1812
SstI	GAGCTC	2 cut(s)	278, 846
StyD4I	CCNGG	6 cut(s)	1023, 1035, 1276, 1678, 1734, 1780
StyI	CCWWGG	4 cut(s)	194, 395, 681, 819
TaaI	ACNGT	4 cut(s)	1015, 1021, 1045, 1824
TaiI	ACGT	2 cut(s)	1622, 1643
TaqI	TCGA	5 cut(s)	175, 186, 450, 588, 633
TaqII	GACCGA	1 cut(s)	468
TauI	GCSGC	1 cut(s)	1195
TfiI	GAWTC	5 cut(s)	172, 815, 1234, 1459, 1570
Tru1I	TTAA	5 cut(s)	203, 462, 549, 1056, 1878
Tru9I	TTAA	5 cut(s)	203, 462, 549, 1056, 1878
TscAI	CASTG	6 cut(s)	373, 633, 1018, 1024, 1137, 1528
TseFI	GTSAC	5 cut(s)	1015, 1138, 1228, 1240, 1478
TseI	GCWGC	6 cut(s)	11, 707, 1064, 1319, 1331, 1395
Tsp45I	GTSAC	5 cut(s)	1015, 1138, 1228, 1240, 1478
TspDTI	ATGAA	5 cut(s)	51, 75, 105, 567, 1116
TspGWI	ACGGA	1 cut(s)	606
TspRI	CASTG	6 cut(s)	373, 633, 1018, 1024, 1137, 1528
Van91I	CCANNNNNTGG	3 cut(s)	1523, 1679, 1763
VpaK11BI	GGWCC	1 cut(s)	430
VspI	ATTAAT	1 cut(s)	549
XagI	CCTNNNNNAGG	2 cut(s)	425, 1683
XapI	RAATTY	2 cut(s)	499, 1866
XbaI	TCTAGA	1 cut(s)	878
XceI	RCATGY	2 cut(s)	1350, 1885
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1284
XmaJI	CCTAGG	2 cut(s)	194, 395
XmiI	GTMKAC	1 cut(s)	588
XspI	CTAG	7 cut(s)	195, 396, 438, 791, 879, 1473, 1812
Zsp2I	ATGCAT	2 cut(s)	1348, 1543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.