MD12G1201300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0018454
Physical Location & Seq
Reverse (-)
28186440 .. 28186787
348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1201300.v1.1.491

Sequence Viewer

Length: 348 bp
ATGGAGAGAGTGAGTAAGCTGGCGCTGCTCCTGACCACCATCCTGGTGGTGGCTGCAATGCCGGTTGAGGGAGGCAGGGTGCTCAAGAGCAACGAAAAGGGGGAGGTTGTTGCTCAGCCTCAGAACGTTTACGGCGGTGTAGGCGGTATCTACCCAGGCTCACCAGGCTTTACTGGGATAGGGTTTGGGCCGTCAGGCTTCTGCTCTCTCCCTGGAGGCTGTGTCCCTACAGGCGGTACAGGTTTTGGCTCAGTCCCACTCCTGCCCACCAATACCCCTGGTGTTCCTCTTGGCGGCGGTGGTATTCCTTTAGGTGCAGGTGGTGGCTCAGCTCCAGTTGTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

11.0

Weight (kDa)

7.78

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018454)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g08340
malus_domestica MD12G1201300.v1.1
prunus_persica Prupe.6G306400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0458221
rosa_roxburghii Rroxscaffold_6G00421210
rosa_rugosa Rorug03G0025500
rosa_samantha Rh3AG086300 Rh3BG089000 Rh3CG089600 Rh3DG090100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 308
Acc36I ACCTGC 1 cut(s) 308
AciI CCGC 5 cut(s) 135, 144, 234, 294, 297
AclI AACGTT 1 cut(s) 126
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 4 cut(s) 49, 68, 233, 293
AjnI CCWGG 5 cut(s) 42, 154, 163, 211, 277
AluBI AGCT 2 cut(s) 19, 332
AluI AGCT 2 cut(s) 19, 332
Alw21I GWGCWC 1 cut(s) 84
AoxI GGCC 1 cut(s) 188
ApeKI GCWGC 2 cut(s) 25, 53
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 12, 40
BccI CCATC 1 cut(s) 47
BceAI ACGGC 2 cut(s) 148, 175
BciT130I CCWGG 5 cut(s) 44, 156, 165, 213, 279
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 1 cut(s) 26
BfuAI ACCTGC 1 cut(s) 308
BisI GCNGC 3 cut(s) 26, 54, 295
BlpI GCTNAGC 2 cut(s) 114, 328
BlsI GCNGC 3 cut(s) 27, 55, 296
Bme1390I CCNGG 5 cut(s) 44, 156, 165, 213, 279
BmgT120I GGNCC 1 cut(s) 188
BmrFI CCNGG 5 cut(s) 44, 156, 165, 213, 279
BmrI ACTGGG 1 cut(s) 183
BmuI ACTGGG 1 cut(s) 183
BpmI CTGGAG 2 cut(s) 234, 318
Bpu1102I GCTNAGC 2 cut(s) 114, 328
BpuEI CTTGAG 1 cut(s) 68
BsaJI CCNNGG 3 cut(s) 154, 211, 277
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 207, 237
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 68, 233, 293
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 2 cut(s) 178, 335
Bse3DI GCAATG 1 cut(s) 63
BseBI CCWGG 5 cut(s) 44, 156, 165, 213, 279
BseDI CCNNGG 3 cut(s) 154, 211, 277
BseGI GGATG 1 cut(s) 39
BseLI CCNNNNNNNGG 4 cut(s) 49, 68, 233, 293
BseMI GCAATG 1 cut(s) 63
BseMII CTCAG 4 cut(s) 128, 134, 264, 342
BseNI ACTGG 2 cut(s) 178, 335
BseXI GCAGC 2 cut(s) 12, 40
BsgI GTGCAG 1 cut(s) 336
BshFI GGCC 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 2 cut(s) 209, 239
BslI CCNNNNNNNGG 4 cut(s) 49, 68, 233, 293
BsmFI GGGAC 2 cut(s) 209, 239
BsnI GGCC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 84
Bsp1720I GCTNAGC 2 cut(s) 114, 328
BspACI CCGC 5 cut(s) 135, 144, 234, 294, 297
BspANI GGCC 1 cut(s) 190
BspCNI CTCAG 4 cut(s) 127, 133, 263, 341
BspMI ACCTGC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 63
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 2 cut(s) 178, 335
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 3 cut(s) 154, 211, 277
Bst2UI CCWGG 5 cut(s) 44, 156, 165, 213, 279
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 4 cut(s) 114, 120, 250, 328
BstF5I GGATG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 26
BstHHI GCGC 1 cut(s) 25
BstMWI GCNNNNNNNGC 3 cut(s) 25, 141, 165
BstNI CCWGG 5 cut(s) 44, 156, 165, 213, 279
BstSCI CCNGG 5 cut(s) 42, 154, 163, 211, 277
BstSFI CTRYAG 1 cut(s) 228
BstV1I GCAGC 2 cut(s) 12, 40
BstXI CCANNNNNNTGG 2 cut(s) 43, 46
BsuRI GGCC 1 cut(s) 190
BtsCI GGATG 1 cut(s) 39
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 21
CfoI GCGC 1 cut(s) 25
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 237
CviAII CATG 1 cut(s) 345
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 4 cut(s) 114, 120, 250, 328
EcoRII CCWGG 5 cut(s) 42, 154, 163, 211, 277
FaeI CATG 1 cut(s) 348
FaiI YATR 1 cut(s) 346
FaqI GGGAC 2 cut(s) 209, 239
FatI CATG 1 cut(s) 344
Fnu4HI GCNGC 3 cut(s) 26, 54, 295
FokI GGATG 1 cut(s) 26
Fsp4HI GCNGC 3 cut(s) 26, 54, 295
GlaI GCGC 1 cut(s) 24
GluI GCNGC 3 cut(s) 26, 54, 295
GsuI CTGGAG 2 cut(s) 234, 318
HaeII RGCGCY 1 cut(s) 26
HaeIII GGCC 1 cut(s) 190
HapII CCGG 1 cut(s) 62
HhaI GCGC 1 cut(s) 25
Hin1II CATG 1 cut(s) 348
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HpaII CCGG 1 cut(s) 62
HphI GGTGA 1 cut(s) 153
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 123
Hpy188III TCNNGA 2 cut(s) 31, 85
Hpy8I GTNNAC 1 cut(s) 130
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 2 cut(s) 56, 317
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 141, 165
HpyF3I CTNAG 4 cut(s) 114, 120, 250, 328
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 1 cut(s) 348
HspAI GCGC 1 cut(s) 23
LmnI GCTCC 2 cut(s) 33, 337
Lsp1109I GCAGC 2 cut(s) 12, 40
MaeII ACGT 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 84
MnlI CCTC 6 cut(s) 61, 65, 97, 129, 209, 297
MslI CAYNNNNRTG 1 cut(s) 44
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 5 cut(s) 44, 156, 165, 213, 279
MvaI CCWGG 5 cut(s) 44, 156, 165, 213, 279
MwoI GCNNNNNNNGC 3 cut(s) 25, 141, 165
NlaIII CATG 1 cut(s) 348
PaqCI CACCTGC 1 cut(s) 308
PkrI GCNGC 3 cut(s) 27, 55, 296
Psp1406I AACGTT 1 cut(s) 126
Psp6I CCWGG 5 cut(s) 42, 154, 163, 211, 277
PspGI CCWGG 5 cut(s) 42, 154, 163, 211, 277
PspPI GGNCC 1 cut(s) 188
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
RseI CAYNNNNRTG 1 cut(s) 44
SatI GCNGC 3 cut(s) 26, 54, 295
Sau96I GGNCC 1 cut(s) 188
ScrFI CCNGG 5 cut(s) 44, 156, 165, 213, 279
SduI GDGCHC 1 cut(s) 84
SetI ASST 7 cut(s) 21, 108, 129, 244, 316, 322, 334
SfcI CTRYAG 1 cut(s) 228
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
SsiI CCGC 5 cut(s) 135, 144, 234, 294, 297
StyD4I CCNGG 5 cut(s) 42, 154, 163, 211, 277
TaiI ACGT 1 cut(s) 129
TauI GCSGC 1 cut(s) 297
TseI GCWGC 2 cut(s) 25, 53
XcmI CCANNNNNNNNNTGG 2 cut(s) 43, 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.