MD12G1201400.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016872
Physical Location & Seq
Reverse (-)
28198055 .. 28199212
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1201400.v1.1.491

Sequence Viewer

Length: 984 bp
ATGGCTGCTGCCCCAATGTTAAGATTGTGTTTGATATTCTTTTTGGCCATGTCGATGATGGTTTTTGTGTCATCAGCAGCTCCGACAGTTGGGTTGTATGGATCAAGTTGCCCTTCTGCAGAGGCAATCGTGAGAAAAGCCGTGACGAAAGCTGTGGCAAGGAATCCTGGCATCGCTGCTGGCCTCATCAGAATGCATTTCCATGATTGTTTTGTTAGGGGTTGTGATGCTTCTGTCCTACTTGATTCAACTCCCGAAAACCCATCCGAAAAAGAGCATCCAGCAAACTTTCCAAGCCTAAGAGGGTTTGAAGTGATTGATGAGGCAAAGGCAGAACTCGAAGCAAAATGTCCAAATACCGTGTCATGTGCTGACATCCTTGCATTTGCAGCTCGTGACGGCGCACTCCAAGTTGGTGGAATAAACTATGCAGTGCCATCAGGTCGGCGTGACGGGAGAATCTCCCTCCGAGATGAGCCTAACGACCACCTGCCCCCACCTTCCTTCGACGCCAAGCAACTCGAGGAGAATTTTGCTCGAAAAGGCCTTAGCTTGGACGAAATGGTCACATTGTCCGGGGCACATTCTATTGGTGTGACACGCTGTTCTACCATCTTGGATCGCTTGTATTCCTTCAATGCAACTCACCCTACAGATCCATCAATGGATCCCATATTTGCTAGAAACTTGAAGAAGAAGTGCCCTAGATCGTCTGACGATCCAAAAACGGTGCCACTTGATGTTCTTACACCAAATAAGCTAGACAATAAGTACTACACTGATTTGAAGAACCACCAGGGCATGCTTGCATCGGATCAAACGCTTCTCACTAGCCGGTCGACGGCTGGGTTTGTAAGGAACAATGCCAGATTTGGTGCTGCATGGGCTAATAAGTTTGGAGCAGCCATGGTGAAAATGGGATCTATTGATGTCCTCACAGGAAGACAGGGTGAAATCAGGAAGAATTGCAGGGTTGTCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

328

Amino Acids

35.43

Weight (kDa)

8.7

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 44 - 289 1.2e-83 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016872)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g08310
malus_domestica MD12G1201400.v1.1
prunus_persica Prupe.6G306500_v2.0.a1
pyrus_communis pycom12g18750
rosa_chinensis RchiOBHm_Chr3g0458181
rosa_laevigata RLG00000025173
rosa_multiflora Rmu_sc0012767.1_g000002
rosa_roxburghii Rroxscaffold_6G00421230
rosa_rugosa Rorug03G0025400
rosa_samantha Rh3AG086200 Rh3BG088900 Rh3CG089500 Rh3DG089900
rosa_wichuraiana Rw3G007320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 498
AasI GACNNNNNNGTC 1 cut(s) 563
Acc36I ACCTGC 1 cut(s) 498
AccB1I GGYRCC 1 cut(s) 730
AccI GTMKAC 1 cut(s) 839
AclWI GGATC 8 cut(s) 109, 627, 650, 662, 675, 713, 822, 928
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 1 cut(s) 529
AcyI GRCGYC 1 cut(s) 510
AfaI GTAC 1 cut(s) 773
AfiI CCNNNNNNNGG 3 cut(s) 89, 553, 841
AgsI TTSAA 5 cut(s) 249, 311, 637, 691, 787
AjnI CCWGG 2 cut(s) 166, 795
AluBI AGCT 5 cut(s) 80, 152, 392, 552, 760
AluI AGCT 5 cut(s) 80, 152, 392, 552, 760
AlwI GGATC 8 cut(s) 109, 627, 650, 662, 675, 713, 822, 928
Ama87I CYCGRG 1 cut(s) 521
AoxI GGCC 3 cut(s) 45, 181, 544
ApeKI GCWGC 7 cut(s) 5, 8, 77, 176, 389, 878, 902
ApoI RAATTY 1 cut(s) 529
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AspLEI GCGC 1 cut(s) 404
AsuC2I CCSGG 1 cut(s) 577
AsuHPI GGTGA 3 cut(s) 638, 922, 962
AvaI CYCGRG 1 cut(s) 521
BaeGI GKGCMC 2 cut(s) 583, 704
BalI TGGCCA 1 cut(s) 47
BamHI GGATCC 1 cut(s) 667
BanI GGYRCC 1 cut(s) 730
BauI CACGAG 1 cut(s) 393
BbsI GAAGAC 1 cut(s) 949
BbvI GCAGC 5 cut(s) 89, 163, 401, 865, 914
BccI CCATC 5 cut(s) 52, 271, 445, 620, 667
BceAI ACGGC 3 cut(s) 125, 415, 858
BciT130I CCWGG 2 cut(s) 168, 797
BcnI CCSGG 1 cut(s) 577
BfaI CTAG 4 cut(s) 681, 705, 761, 831
BfmI CTRYAG 2 cut(s) 117, 651
BfuAI ACCTGC 1 cut(s) 498
BisI GCNGC 7 cut(s) 6, 9, 78, 177, 390, 879, 903
BlsI GCNGC 7 cut(s) 7, 10, 79, 178, 391, 880, 904
BmcAI AGTACT 1 cut(s) 773
Bme1390I CCNGG 3 cut(s) 168, 577, 797
BmeT110I CYCGRG 1 cut(s) 521
BmiI GGNNCC 2 cut(s) 669, 732
BmrFI CCNGG 3 cut(s) 168, 577, 797
BmsI GCATC 4 cut(s) 180, 217, 286, 818
BpiI GAAGAC 1 cut(s) 949
Bpu10I CCTNAGC 1 cut(s) 548
BpuMI CCSGG 1 cut(s) 577
BsaHI GRCGYC 1 cut(s) 510
BsaJI CCNNGG 3 cut(s) 576, 796, 906
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 553, 841
Bse118I RCCGGY 1 cut(s) 834
BseBI CCWGG 2 cut(s) 168, 797
BseDI CCNNGG 3 cut(s) 576, 796, 906
BseGI GGATG 3 cut(s) 263, 277, 375
BseLI CCNNNNNNNGG 3 cut(s) 89, 553, 841
BseRI GAGGAG 1 cut(s) 539
BseSI GKGCMC 2 cut(s) 583, 704
BseXI GCAGC 5 cut(s) 89, 163, 401, 865, 914
BseYI CCCAGC 1 cut(s) 845
Bsh1285I CGRYCG 1 cut(s) 839
BshFI GGCC 3 cut(s) 47, 183, 546
BshNI GGYRCC 1 cut(s) 730
BsiEI CGRYCG 1 cut(s) 839
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 2 cut(s) 576, 835
BslI CCNNNNNNNGG 3 cut(s) 89, 553, 841
BsmI GAATGC 1 cut(s) 198
BsnI GGCC 3 cut(s) 47, 183, 546
BsoBI CYCGRG 1 cut(s) 521
Bsp1286I GDGCHC 2 cut(s) 583, 704
Bsp143I GATC 8 cut(s) 101, 619, 655, 667, 707, 718, 814, 920
Bsp19I CCATGG 1 cut(s) 906
BspANI GGCC 3 cut(s) 47, 183, 546
BspLI GGNNCC 2 cut(s) 669, 732
BspMAI CTGCAG 1 cut(s) 121
BspMI ACCTGC 1 cut(s) 498
BspPI GGATC 8 cut(s) 109, 627, 650, 662, 675, 713, 822, 928
BspT107I GGYRCC 1 cut(s) 730
BsrFI RCCGGY 1 cut(s) 834
BssAI RCCGGY 1 cut(s) 834
BssECI CCNNGG 3 cut(s) 576, 796, 906
BssMI GATC 8 cut(s) 101, 619, 655, 667, 707, 718, 814, 920
BssNI GRCGYC 1 cut(s) 510
BssSI CACGAG 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 906
Bst2BI CACGAG 1 cut(s) 393
Bst2UI CCWGG 2 cut(s) 168, 797
Bst4CI ACNGT 3 cut(s) 88, 361, 730
BstACI GRCGYC 1 cut(s) 510
BstC8I GCNNGC 3 cut(s) 181, 803, 807
BstDEI CTNAG 2 cut(s) 299, 548
BstDSI CCRYGG 1 cut(s) 906
BstF5I GGATG 3 cut(s) 263, 277, 375
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 8 cut(s) 104, 622, 658, 670, 710, 721, 817, 923
BstMBI GATC 8 cut(s) 101, 619, 655, 667, 707, 718, 814, 920
BstMCI CGRYCG 1 cut(s) 839
BstMWI GCNNNNNNNGC 2 cut(s) 389, 884
BstNI CCWGG 2 cut(s) 168, 797
BstNSI RCATGY 1 cut(s) 805
BstSCI CCNGG 3 cut(s) 166, 575, 795
BstSFI CTRYAG 2 cut(s) 117, 651
BstSLI GKGCMC 2 cut(s) 583, 704
BstV1I GCAGC 5 cut(s) 89, 163, 401, 865, 914
BstV2I GAAGAC 1 cut(s) 949
BstX2I RGATCY 3 cut(s) 655, 667, 920
BstXI CCANNNNNNTGG 1 cut(s) 416
BstYI RGATCY 3 cut(s) 655, 667, 920
BsuRI GGCC 3 cut(s) 47, 183, 546
BtgI CCRYGG 1 cut(s) 906
BtgZI GCGATG 1 cut(s) 157
BtsCI GGATG 3 cut(s) 263, 277, 375
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 2 cut(s) 438, 777
BveI ACCTGC 1 cut(s) 498
Cac8I GCNNGC 3 cut(s) 181, 803, 807
CfoI GCGC 1 cut(s) 404
Cfr10I RCCGGY 1 cut(s) 834
CseI GACGC 1 cut(s) 518
Csp6I GTAC 1 cut(s) 772
CviAII CATG 6 cut(s) 49, 203, 366, 802, 882, 907
CviQI GTAC 1 cut(s) 772
DdeI CTNAG 2 cut(s) 299, 548
DpnI GATC 8 cut(s) 103, 621, 657, 669, 709, 720, 816, 922
DpnII GATC 8 cut(s) 101, 619, 655, 667, 707, 718, 814, 920
DrdI GACNNNNNNGTC 1 cut(s) 563
DseDI GACNNNNNNGTC 1 cut(s) 563
EaeI YGGCCR 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 906
Eco147I AGGCCT 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 521
EcoRII CCWGG 2 cut(s) 166, 795
EcoT14I CCWWGG 1 cut(s) 906
EcoT22I ATGCAT 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 906
FaeI CATG 6 cut(s) 52, 206, 369, 805, 885, 910
FaiI YATR 9 cut(s) 50, 99, 204, 367, 429, 674, 803, 883, 908
FalI AAGNNNNNCTT 2 cut(s) 97, 129
FatI CATG 6 cut(s) 48, 202, 365, 801, 881, 906
FblI GTMKAC 1 cut(s) 839
Fnu4HI GCNGC 7 cut(s) 6, 9, 78, 177, 390, 879, 903
FokI GGATG 3 cut(s) 250, 264, 362
Fsp4HI GCNGC 7 cut(s) 6, 9, 78, 177, 390, 879, 903
FspBI CTAG 4 cut(s) 681, 705, 761, 831
GlaI GCGC 1 cut(s) 403
GluI GCNGC 7 cut(s) 6, 9, 78, 177, 390, 879, 903
GsaI CCCAGC 1 cut(s) 849
HaeIII GGCC 3 cut(s) 47, 183, 546
HapII CCGG 2 cut(s) 576, 835
HgaI GACGC 1 cut(s) 518
HhaI GCGC 1 cut(s) 404
Hin1I GRCGYC 1 cut(s) 510
Hin1II CATG 6 cut(s) 52, 206, 369, 805, 885, 910
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HincII GTYRAC 1 cut(s) 840
HindII GTYRAC 1 cut(s) 840
HinfI GANTC 3 cut(s) 163, 245, 459
HpaII CCGG 2 cut(s) 576, 835
HphI GGTGA 3 cut(s) 638, 922, 962
Hpy166II GTNNAC 1 cut(s) 840
Hpy188I TCNGA 6 cut(s) 84, 191, 268, 470, 715, 814
Hpy188III TCNNGA 4 cut(s) 130, 254, 395, 958
Hpy8I GTNNAC 1 cut(s) 840
Hpy99I CGWCG 2 cut(s) 512, 844
HpyAV CCTTC 4 cut(s) 123, 510, 514, 643
HpyCH4III ACNGT 3 cut(s) 88, 361, 730
HpyCH4V TGCA 9 cut(s) 119, 196, 383, 389, 431, 641, 809, 881, 969
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 884
HpyF3I CTNAG 2 cut(s) 299, 548
Hsp92I GRCGYC 1 cut(s) 510
Hsp92II CATG 6 cut(s) 52, 206, 369, 805, 885, 910
HspAI GCGC 1 cut(s) 402
Kzo9I GATC 8 cut(s) 101, 619, 655, 667, 707, 718, 814, 920
LmnI GCTCC 2 cut(s) 85, 899
Lsp1109I GCAGC 5 cut(s) 89, 163, 401, 865, 914
LweI GCATC 4 cut(s) 180, 217, 286, 818
MaeI CTAG 4 cut(s) 681, 705, 761, 831
MaeIII GTNAC 5 cut(s) 142, 395, 449, 565, 595
MalI GATC 8 cut(s) 103, 621, 657, 669, 709, 720, 816, 922
MboI GATC 8 cut(s) 101, 619, 655, 667, 707, 718, 814, 920
MboII GAAGA 5 cut(s) 703, 706, 799, 954, 973
MflI RGATCY 3 cut(s) 655, 667, 920
MhlI GDGCHC 2 cut(s) 583, 704
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 3 cut(s) 529, 964, 979
MluNI TGGCCA 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 7 cut(s) 115, 194, 296, 316, 476, 517, 944
Mox20I TGGCCA 1 cut(s) 47
Mph1103I ATGCAT 1 cut(s) 198
MscI TGGCCA 1 cut(s) 47
MseI TTAA 1 cut(s) 20
MslI CAYNNNNRTG 3 cut(s) 53, 191, 201
Msp20I TGGCCA 1 cut(s) 47
MspI CCGG 2 cut(s) 576, 835
MspR9I CCNGG 3 cut(s) 168, 577, 797
Mva1269I GAATGC 1 cut(s) 198
MvaI CCWGG 2 cut(s) 168, 797
MwoI GCNNNNNNNGC 2 cut(s) 389, 884
NciI CCSGG 1 cut(s) 577
NcoI CCATGG 1 cut(s) 906
NdeII GATC 8 cut(s) 101, 619, 655, 667, 707, 718, 814, 920
NlaIII CATG 6 cut(s) 52, 206, 369, 805, 885, 910
NlaIV GGNNCC 2 cut(s) 669, 732
NmuCI GTSAC 5 cut(s) 142, 395, 449, 565, 595
NsiI ATGCAT 1 cut(s) 198
NspI RCATGY 1 cut(s) 805
PaeI GCATGC 1 cut(s) 805
PaeR7I CTCGAG 1 cut(s) 521
PaqCI CACCTGC 1 cut(s) 498
PceI AGGCCT 1 cut(s) 546
PctI GAATGC 1 cut(s) 198
PfeI GAWTC 3 cut(s) 163, 245, 459
PkrI GCNGC 7 cut(s) 7, 10, 79, 178, 391, 880, 904
Psp6I CCWGG 2 cut(s) 166, 795
PspFI CCCAGC 1 cut(s) 845
PspGI CCWGG 2 cut(s) 166, 795
PspN4I GGNNCC 2 cut(s) 669, 732
PspXI VCTCGAGB 1 cut(s) 521
PstI CTGCAG 1 cut(s) 121
PsuI RGATCY 3 cut(s) 655, 667, 920
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 3 cut(s) 53, 191, 201
SalI GTCGAC 1 cut(s) 838
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 7 cut(s) 6, 9, 78, 177, 390, 879, 903
Sau3AI GATC 8 cut(s) 101, 619, 655, 667, 707, 718, 814, 920
ScaI AGTACT 1 cut(s) 773
ScrFI CCNGG 3 cut(s) 168, 577, 797
SduI GDGCHC 2 cut(s) 583, 704
SetI ASST 8 cut(s) 82, 154, 394, 445, 492, 502, 554, 762
SfaNI GCATC 4 cut(s) 180, 217, 286, 818
SfcI CTRYAG 2 cut(s) 117, 651
Sfr274I CTCGAG 1 cut(s) 521
SlaI CTCGAG 1 cut(s) 521
SmiMI CAYNNNNRTG 3 cut(s) 53, 191, 201
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
SphI GCATGC 1 cut(s) 805
Sse9I AATT 3 cut(s) 529, 964, 979
SseBI AGGCCT 1 cut(s) 546
SspMI CTAG 4 cut(s) 681, 705, 761, 831
StuI AGGCCT 1 cut(s) 546
StyD4I CCNGG 3 cut(s) 166, 575, 795
StyI CCWWGG 1 cut(s) 906
TaaI ACNGT 3 cut(s) 88, 361, 730
TaqI TCGA 6 cut(s) 53, 339, 507, 522, 538, 839
TasI AATT 3 cut(s) 529, 964, 979
TatI WGTACW 1 cut(s) 771
TfiI GAWTC 3 cut(s) 163, 245, 459
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 2 cut(s) 438, 784
TseFI GTSAC 5 cut(s) 142, 395, 449, 565, 595
TseI GCWGC 7 cut(s) 5, 8, 77, 176, 389, 878, 902
Tsp45I GTSAC 5 cut(s) 142, 395, 449, 565, 595
TspRI CASTG 2 cut(s) 438, 784
XapI RAATTY 1 cut(s) 529
XceI RCATGY 1 cut(s) 805
XcmI CCANNNNNNNNNTGG 2 cut(s) 55, 913
XhoI CTCGAG 1 cut(s) 521
XmiI GTMKAC 1 cut(s) 839
XspI CTAG 4 cut(s) 681, 705, 761, 831
ZrmI AGTACT 1 cut(s) 773
Zsp2I ATGCAT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.