Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0017324

Physical Location & Seq

Genomic Coordinates Forward (+)

28711258 .. 28713799

Gene Structure 2542 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1206800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 525 bp

ATGCCAGATAAGCCGCGAGTGCACAAGTATGCCATATGGGCCGTCCCACCGGATGATGTGTCGGAGAGGATCAAGAAGGTGATGGACGGTCTTCGCGAAGAATTTGGTGGGCCGGAGATCCGACCCCACATTCCGATTATGGTGTCGATCCACTCGGATCACGAGTCCATCGTGAAGAAGTTCAGAGAGATATGTACGGGAGTCAGTTACGATTGTAAAATTGAGCGCGTGAATACCAGTCCATGTGCATTCTATTACCAGTGCGTTTACCTCTTCATCGATCCCGAGGTAATTCATATGAGGAACGTGGCTGGGAGATTTGGACGCTTCGGTCCTATGCCACATTTGAGCCTCCTCTATGGGCATCTGACCGACGAAGAGAAGCAAAAGGCTAAGGAAAAAGTTCTTGCTCTTGATGGTGGCATCGTTGGTCTCCGTTTCACGATGAGACGCCTTGTTTTGTACAAGTGTAACGACGATATGACTCAACAGACTTGGGAGAAGGTTGCTGAAGTTTTTGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000394 GO:0003674 GO:0003824 GO:0004112 GO:0004113 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006388 GO:0006396 GO:0006399 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008033 GO:0008081 GO:0008150 GO:0008152 GO:0008380 GO:0009117 GO:0009187 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0019637 GO:0034470 GO:0034641 GO:0034660 GO:0042578 GO:0043170 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044464 GO:0046483 GO:0055086 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2_5_RNA_ligase2 CPDase

Protein Analysis

175

Amino Acids

20.1

Weight (kDa)

7.68

Isoelectric Point (pI)

42.35

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017324)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g07730
malus_domestica	MD12G1206800.v1.1
prunus_persica	Prupe.6G314300_v2.0.a1
pyrus_communis	pycom12g19470
rosa_chinensis	RchiOBHm_Chr3g0457371
rosa_multiflora	Rmu_sc0005442.1_g000004
rosa_roxburghii	Rroxscaffold_6G00421940
rosa_rugosa	Rorug03G0020200 Rorug03G0020300
rosa_samantha	Rh3AG080400 Rh3BG082200 Rh3CG082500
rosa_wichuraiana	Rw3G006910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	330
AccII	CGCG	3 cut(s)	16, 96, 228
AciI	CCGC	1 cut(s)	14
AclWI	GGATC	5 cut(s)	77, 112, 142, 165, 275
AcsI	RAATTY	1 cut(s)	101
AcyI	GRCGYC	1 cut(s)	451
AfaI	GTAC	2 cut(s)	196, 464
Alw21I	GWGCWC	1 cut(s)	24
Alw26I	GTCTC	2 cut(s)	437, 442
Alw44I	GTGCAC	1 cut(s)	20
AlwI	GGATC	5 cut(s)	77, 112, 142, 165, 275
Ama87I	CYCGRG	1 cut(s)	284
AoxI	GGCC	2 cut(s)	39, 110
ApaLI	GTGCAC	1 cut(s)	20
ApoI	RAATTY	1 cut(s)	101
Asp700I	GAANNNNTTC	2 cut(s)	179, 402
AspLEI	GCGC	1 cut(s)	228
AspS9I	GGNCC	3 cut(s)	39, 110, 332
AsuHPI	GGTGA	1 cut(s)	91
AvaI	CYCGRG	1 cut(s)	284
AvaII	GGWCC	1 cut(s)	332
BaeGI	GKGCMC	1 cut(s)	24
BauI	CACGAG	1 cut(s)	161
BbsI	GAAGAC	1 cut(s)	83
Bbv12I	GWGCWC	1 cut(s)	24
BccI	CCATC	3 cut(s)	76, 176, 410
BceAI	ACGGC	1 cut(s)	26
BcoDI	GTCTC	2 cut(s)	437, 442
BglI	GCCNNNNNGGC	1 cut(s)	38
BisI	GCNGC	1 cut(s)	14
BlsI	GCNGC	1 cut(s)	15
Bme18I	GGWCC	1 cut(s)	332
BmeT110I	CYCGRG	1 cut(s)	284
BmgT120I	GGNCC	3 cut(s)	39, 110, 332
BmsI	GCATC	2 cut(s)	373, 432
BpiI	GAAGAC	1 cut(s)	83
Bpu10I	CCTNAGC	1 cut(s)	393
Bsa29I	ATCGAT	1 cut(s)	279
BsaHI	GRCGYC	1 cut(s)	451
BsaI	GGTCTC	1 cut(s)	437
BsaJI	CCNNGG	1 cut(s)	285
BsaWI	WCCGGW	1 cut(s)	49
Bse1I	ACTGG	2 cut(s)	237, 259
BseCI	ATCGAT	1 cut(s)	279
BseDI	CCNNGG	1 cut(s)	285
BseGI	GGATG	1 cut(s)	58
BseNI	ACTGG	2 cut(s)	237, 259
BseRI	GAGGAG	1 cut(s)	344
BseSI	GKGCMC	1 cut(s)	24
BseYI	CCCAGC	1 cut(s)	311
Bsh1236I	CGCG	3 cut(s)	16, 96, 228
BshFI	GGCC	2 cut(s)	41, 112
BshVI	ATCGAT	1 cut(s)	279
BsiHKAI	GWGCWC	1 cut(s)	24
BsiHKCI	CYCGRG	1 cut(s)	284
BsiSI	CCGG	2 cut(s)	50, 113
BslFI	GGGAC	1 cut(s)	29
BsmAI	GTCTC	2 cut(s)	437, 442
BsmBI	CGTCTC	1 cut(s)	442
BsmFI	GGGAC	1 cut(s)	29
BsmI	GAATGC	1 cut(s)	248
BsnI	GGCC	2 cut(s)	41, 112
Bso31I	GGTCTC	1 cut(s)	437
BsoBI	CYCGRG	1 cut(s)	284
Bsp1286I	GDGCHC	1 cut(s)	24
Bsp1407I	TGTACA	1 cut(s)	462
Bsp143I	GATC	5 cut(s)	69, 117, 147, 157, 280
Bsp68I	TCGCGA	1 cut(s)	96
BspACI	CCGC	1 cut(s)	14
BspANI	GGCC	2 cut(s)	41, 112
BspDI	ATCGAT	1 cut(s)	279
BspFNI	CGCG	3 cut(s)	16, 96, 228
BspPI	GGATC	5 cut(s)	77, 112, 142, 165, 275
BspTNI	GGTCTC	1 cut(s)	437
BsrGI	TGTACA	1 cut(s)	462
BsrI	ACTGG	2 cut(s)	237, 259
BssECI	CCNNGG	1 cut(s)	285
BssMI	GATC	5 cut(s)	69, 117, 147, 157, 280
BssNI	GRCGYC	1 cut(s)	451
BssSI	CACGAG	1 cut(s)	161
Bst2BI	CACGAG	1 cut(s)	161
Bst4CI	ACNGT	1 cut(s)	89
Bst6I	CTCTTC	2 cut(s)	278, 372
BstACI	GRCGYC	1 cut(s)	451
BstAUI	TGTACA	1 cut(s)	462
BstDEI	CTNAG	1 cut(s)	393
BstF5I	GGATG	1 cut(s)	58
BstFNI	CGCG	3 cut(s)	16, 96, 228
BstHHI	GCGC	1 cut(s)	228
BstKTI	GATC	5 cut(s)	72, 120, 150, 160, 283
BstMAI	GTCTC	2 cut(s)	437, 442
BstMBI	GATC	5 cut(s)	69, 117, 147, 157, 280
BstMWI	GCNNNNNNNGC	3 cut(s)	10, 19, 38
BstSLI	GKGCMC	1 cut(s)	24
BstUI	CGCG	3 cut(s)	16, 96, 228
BstV2I	GAAGAC	1 cut(s)	83
BstX2I	RGATCY	1 cut(s)	117
BstYI	RGATCY	1 cut(s)	117
Bsu15I	ATCGAT	1 cut(s)	279
BsuRI	GGCC	2 cut(s)	41, 112
BsuTUI	ATCGAT	1 cut(s)	279
BtsCI	GGATG	1 cut(s)	58
BtsIMutI	CAGTG	1 cut(s)	266
BtuMI	TCGCGA	1 cut(s)	96
CfoI	GCGC	1 cut(s)	228
Cfr13I	GGNCC	3 cut(s)	39, 110, 332
ClaI	ATCGAT	1 cut(s)	279
CseI	GACGC	2 cut(s)	333, 459
Csp6I	GTAC	2 cut(s)	195, 463
CviAII	CATG	1 cut(s)	243
CviJI	RGCY	6 cut(s)	13, 41, 112, 311, 351, 392
CviKI_1	RGCY	6 cut(s)	13, 41, 112, 311, 351, 392
CviQI	GTAC	2 cut(s)	195, 463
DdeI	CTNAG	1 cut(s)	393
DpnI	GATC	5 cut(s)	71, 119, 149, 159, 282
DpnII	GATC	5 cut(s)	69, 117, 147, 157, 280
DrdI	GACNNNNNNGTC	1 cut(s)	330
DseDI	GACNNNNNNGTC	1 cut(s)	330
Eam1104I	CTCTTC	2 cut(s)	278, 372
EarI	CTCTTC	2 cut(s)	278, 372
Eco31I	GGTCTC	1 cut(s)	437
Eco47I	GGWCC	1 cut(s)	332
Eco88I	CYCGRG	1 cut(s)	284
Esp3I	CGTCTC	1 cut(s)	442
FaeI	CATG	1 cut(s)	246
FaqI	GGGAC	1 cut(s)	29
FatI	CATG	1 cut(s)	242
FauNDI	CATATG	2 cut(s)	35, 297
Fnu4HI	GCNGC	1 cut(s)	14
FokI	GGATG	1 cut(s)	65
Fsp4HI	GCNGC	1 cut(s)	14
GlaI	GCGC	1 cut(s)	227
GluI	GCNGC	1 cut(s)	14
GsaI	CCCAGC	1 cut(s)	315
HaeIII	GGCC	2 cut(s)	41, 112
HapII	CCGG	2 cut(s)	50, 113
HgaI	GACGC	2 cut(s)	333, 459
HhaI	GCGC	1 cut(s)	228
Hin1I	GRCGYC	1 cut(s)	451
Hin1II	CATG	1 cut(s)	246
Hin6I	GCGC	1 cut(s)	226
HinP1I	GCGC	1 cut(s)	226
HinfI	GANTC	3 cut(s)	164, 201, 484
HpaII	CCGG	2 cut(s)	50, 113
HphI	GGTGA	1 cut(s)	91
Hpy166II	GTNNAC	2 cut(s)	22, 268
Hpy188I	TCNGA	6 cut(s)	64, 122, 135, 157, 185, 369
Hpy188III	TCNNGA	7 cut(s)	73, 95, 161, 172, 284, 413, 442
Hpy8I	GTNNAC	2 cut(s)	22, 268
Hpy99I	CGWCG	2 cut(s)	377, 479
HpyAV	CCTTC	2 cut(s)	70, 496
HpyCH4III	ACNGT	1 cut(s)	89
HpyCH4IV	ACGT	1 cut(s)	306
HpyCH4V	TGCA	2 cut(s)	22, 248
HpyF10VI	GCNNNNNNNGC	3 cut(s)	10, 19, 38
HpyF3I	CTNAG	1 cut(s)	393
HpySE526I	ACGT	1 cut(s)	306
Hsp92I	GRCGYC	1 cut(s)	451
Hsp92II	CATG	1 cut(s)	246
HspAI	GCGC	1 cut(s)	226
Kzo9I	GATC	5 cut(s)	69, 117, 147, 157, 280
LpnPI	CCDG	6 cut(s)	18, 63, 126, 250, 272, 297
LweI	GCATC	2 cut(s)	373, 432
MaeII	ACGT	1 cut(s)	306
MaeIII	GTNAC	2 cut(s)	206, 470
MalI	GATC	5 cut(s)	71, 119, 149, 159, 282
MboI	GATC	5 cut(s)	69, 117, 147, 157, 280
MboII	GAAGA	5 cut(s)	83, 110, 187, 265, 389
MflI	RGATCY	1 cut(s)	117
MhlI	GDGCHC	1 cut(s)	24
MluCI	AATT	3 cut(s)	101, 219, 291
MlyI	GAGTC	3 cut(s)	173, 210, 478
MmeI	TCCRAC	2 cut(s)	42, 145
MnlI	CCTC	6 cut(s)	60, 280, 281, 294, 362, 365
MroXI	GAANNNNTTC	2 cut(s)	179, 402
MslI	CAYNNNNRTG	1 cut(s)	27
MspI	CCGG	2 cut(s)	50, 113
Mva1269I	GAATGC	1 cut(s)	248
MvnI	CGCG	3 cut(s)	16, 96, 228
MwoI	GCNNNNNNNGC	3 cut(s)	10, 19, 38
NdeI	CATATG	2 cut(s)	35, 297
NdeII	GATC	5 cut(s)	69, 117, 147, 157, 280
NlaIII	CATG	1 cut(s)	246
NruI	TCGCGA	1 cut(s)	96
PcsI	WCGNNNNNNNCGW	2 cut(s)	93, 168
PctI	GAATGC	1 cut(s)	248
PdmI	GAANNNNTTC	2 cut(s)	179, 402
PkrI	GCNGC	1 cut(s)	15
PleI	GAGTC	3 cut(s)	172, 209, 478
PpsI	GAGTC	3 cut(s)	172, 209, 478
PspFI	CCCAGC	1 cut(s)	311
PspPI	GGNCC	3 cut(s)	39, 110, 332
PsuI	RGATCY	1 cut(s)	117
RruI	TCGCGA	1 cut(s)	96
RsaI	GTAC	2 cut(s)	196, 464
RsaNI	GTAC	2 cut(s)	195, 463
RseI	CAYNNNNRTG	1 cut(s)	27
SatI	GCNGC	1 cut(s)	14
Sau3AI	GATC	5 cut(s)	69, 117, 147, 157, 280
Sau96I	GGNCC	3 cut(s)	39, 110, 332
SchI	GAGTC	3 cut(s)	173, 210, 478
SduI	GDGCHC	1 cut(s)	24
SetI	ASST	5 cut(s)	81, 273, 291, 309, 507
SfaNI	GCATC	2 cut(s)	373, 432
SinI	GGWCC	1 cut(s)	332
SmiMI	CAYNNNNRTG	1 cut(s)	27
Sse9I	AATT	3 cut(s)	101, 219, 291
SsiI	CCGC	1 cut(s)	14
TaaI	ACNGT	1 cut(s)	89
TaiI	ACGT	1 cut(s)	309
TaqI	TCGA	2 cut(s)	146, 279
TaqII	GACCGA	2 cut(s)	320, 386
TasI	AATT	3 cut(s)	101, 219, 291
TatI	WGTACW	1 cut(s)	462
TauI	GCSGC	1 cut(s)	16
TscAI	CASTG	1 cut(s)	266
TspDTI	ATGAA	2 cut(s)	265, 284
TspGWI	ACGGA	1 cut(s)	425
TspRI	CASTG	1 cut(s)	266
VneI	GTGCAC	1 cut(s)	20
VpaK11BI	GGWCC	1 cut(s)	332
XapI	RAATTY	1 cut(s)	101
XmnI	GAANNNNTTC	2 cut(s)	179, 402

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.