MD12G1216700.v1.1

PsbP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012050
Physical Location & Seq
Reverse (-)
29426132 .. 29427388
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1216700.v1.1.491

Sequence Viewer

Length: 897 bp
ATGCCGGCATTGCCCCAATACTTTCATGCATGCAAACATATATGCCTCAACCTGAACCAGATACGCATAATCACACCGTCCTCCGGCTGCCGGAAAAAGGGTTTGCGAGAGGTGAGAATAATCCGGGCAGAAAGGCCTCCGGCGGACCAATTTGCTTCACTACAATCAGGGTTTAGGCGGCGGCTTCTTCTCGGTTTAGGGACAGCTTCTGTGGTTGCTCTTGGTGCTGATTTTCTCGGAGTCACAAGTTTTCTTCTCGGGTTGTCGCCGGAAAGTAGTAGGAGTCTTAAGCTGGATGTGGTTTATCCCATAGGAGGCTACAGTAGGTGCATTGACACAAATGAAGGATTTGAATTTATATACCCAGCAAGTTGGGTTGGAGATCAAACACTTCTGTATCGAGCAGCAGAGAAAAAGGAATTTGAAAGATCACTTGATCCTCCTCCCTTGAACTTCAACACCAAATCCAGGACTGATGGCCGCCGACGAAATGTCAGTGAACCCGTTGTCGCATTTGGCCCGCCCGCAACCACCGGCGAGCTCAATGTTAGCGTCATTGTGTCGCCAGTTGCTCCTGATTTCAAAATTGAAGCATTTGGAGGACCAAAAGAGGTAGGAGAAGCCGTGGTTAGGACCGTCACAGGATCCGGCAAACGCCCCGGCATCAAGGGAACTTTGATCGAATCAAAATTGAGAGAGGATAGTGCAAGCAATGTTAAGTACTACGAGCTTGAATTCAGAGTTGAGAGCCCCTTGTTTCACCGGCATAACATCGCAGTATGTTGCGTTCGTGGTGGTCGGTTATTTACACTTAATGCTCAGGCACCGGAATCAGCATGGCCGGAGTTGAAGTCTGACTTCCATAGAATTGCCGAATCGTTTAGCCTCACCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0009579 GO:0031977 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044436 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PsbP

Protein Analysis

299

Amino Acids

32.84

Weight (kDa)

9.25

Isoelectric Point (pI)

52.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbP PF01789 106 - 295 9.5e-29 PsbP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05410 AT3G05410 AT3G05410
fragaria_vesca FvH4_6g06280
malus_domestica MD04G1202900.v1.1 MD12G1216700.v1.1
prunus_persica Prupe.6G323000_v2.0.a1
pyrus_communis pycom04g17920 pycom12g20220
rosa_chinensis RchiOBHm_Chr3g0455321
rosa_laevigata RLG00000025370
rosa_multiflora Rmu_sc0002169.1_g000042
rosa_roxburghii Rroxscaffold_6G00424380
rosa_rugosa Rorug03G0006200.1
rosa_samantha Rh3AG066000 Rh3BG068100 Rh3CG066500 Rh3DG068100
rosa_wichuraiana Rw3G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 823
AciI CCGC 6 cut(s) 143, 178, 181, 481, 521, 525
AclWI GGATC 3 cut(s) 431, 639, 652
AcoI YGGCCR 2 cut(s) 478, 839
AcsI RAATTY 3 cut(s) 353, 419, 734
AfaI GTAC 1 cut(s) 722
AfiI CCNNNNNNNGG 6 cut(s) 83, 90, 97, 314, 468, 630
AflII CTTAAG 1 cut(s) 287
AgsI TTSAA 8 cut(s) 353, 425, 451, 457, 583, 590, 734, 850
AhdI GACNNNNNGTC 1 cut(s) 491
AjnI CCWGG 1 cut(s) 467
AluBI AGCT 4 cut(s) 206, 292, 541, 730
AluI AGCT 4 cut(s) 206, 292, 541, 730
Alw21I GWGCWC 1 cut(s) 543
AlwI GGATC 3 cut(s) 431, 639, 652
AlwNI CAGNNNCTG 1 cut(s) 209
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 4 cut(s) 134, 478, 517, 839
ApeKI GCWGC 2 cut(s) 87, 404
ApoI RAATTY 3 cut(s) 353, 419, 734
AspS9I GGNCC 4 cut(s) 145, 518, 602, 633
AsuC2I CCSGG 2 cut(s) 125, 660
AsuHPI GGTGA 3 cut(s) 124, 752, 880
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 3 cut(s) 145, 602, 633
BaeI ACNNNNGTAYC 2 cut(s) 380, 413
BamHI GGATCC 1 cut(s) 644
BanI GGYRCC 1 cut(s) 823
BanII GRGCYC 2 cut(s) 543, 752
Bbv12I GWGCWC 1 cut(s) 543
BbvI GCAGC 2 cut(s) 74, 416
BccI CCATC 1 cut(s) 470
BceAI ACGGC 1 cut(s) 608
BciT130I CCWGG 1 cut(s) 469
BcnI CCSGG 2 cut(s) 125, 660
BfmI CTRYAG 1 cut(s) 319
BfrI CTTAAG 1 cut(s) 287
BisI GCNGC 5 cut(s) 88, 179, 182, 405, 481
BlsI GCNGC 5 cut(s) 89, 180, 183, 406, 482
BmcAI AGTACT 1 cut(s) 722
Bme1390I CCNGG 3 cut(s) 125, 469, 660
Bme18I GGWCC 3 cut(s) 145, 602, 633
BmeRI GACNNNNNGTC 1 cut(s) 491
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 4 cut(s) 145, 518, 602, 633
BmiI GGNNCC 2 cut(s) 646, 825
BmrFI CCNGG 3 cut(s) 125, 469, 660
BmsI GCATC 1 cut(s) 672
Bpu10I CCTNAGC 1 cut(s) 819
BpuMI CCSGG 2 cut(s) 125, 660
BsaJI CCNNGG 2 cut(s) 624, 658
BsaWI WCCGGW 1 cut(s) 826
BsaXI ACNNNNNCTCC 4 cut(s) 609, 639, 836, 866
Bsc4I CCNNNNNNNGG 6 cut(s) 83, 90, 97, 314, 468, 630
Bse118I RCCGGY 3 cut(s) 4, 533, 762
Bse1I ACTGG 1 cut(s) 566
Bse3DI GCAATG 2 cut(s) 8, 718
BseBI CCWGG 1 cut(s) 469
BseDI CCNNGG 2 cut(s) 624, 658
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 6 cut(s) 83, 90, 97, 314, 468, 630
BseMI GCAATG 2 cut(s) 8, 718
BseMII CTCAG 1 cut(s) 833
BseNI ACTGG 1 cut(s) 566
BseRI GAGGAG 1 cut(s) 432
BseXI GCAGC 2 cut(s) 74, 416
BseYI CCCAGC 1 cut(s) 364
BshFI GGCC 4 cut(s) 136, 480, 519, 841
BshNI GGYRCC 1 cut(s) 823
BsiHKAI GWGCWC 1 cut(s) 543
BsiHKCI CYCGRG 1 cut(s) 257
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 6 cut(s) 83, 90, 97, 314, 468, 630
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 4 cut(s) 136, 480, 519, 841
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 543, 752
Bsp143I GATC 5 cut(s) 382, 428, 436, 644, 678
BspACI CCGC 6 cut(s) 143, 178, 181, 481, 521, 525
BspANI GGCC 4 cut(s) 136, 480, 519, 841
BspCNI CTCAG 1 cut(s) 832
BspLI GGNNCC 2 cut(s) 646, 825
BspPI GGATC 3 cut(s) 431, 639, 652
BspT107I GGYRCC 1 cut(s) 823
BspTI CTTAAG 1 cut(s) 287
BsrDI GCAATG 2 cut(s) 8, 718
BsrFI RCCGGY 3 cut(s) 4, 533, 762
BsrI ACTGG 1 cut(s) 566
BssAI RCCGGY 3 cut(s) 4, 533, 762
BssECI CCNNGG 2 cut(s) 624, 658
BssMI GATC 5 cut(s) 382, 428, 436, 644, 678
Bst2UI CCWGG 1 cut(s) 469
Bst4CI ACNGT 3 cut(s) 78, 323, 637
BstAFI CTTAAG 1 cut(s) 287
BstC8I GCNNGC 6 cut(s) 6, 31, 521, 525, 539, 709
BstDEI CTNAG 1 cut(s) 819
BstDSI CCRYGG 1 cut(s) 624
BstF5I GGATG 1 cut(s) 301
BstKTI GATC 5 cut(s) 385, 431, 439, 647, 681
BstMBI GATC 5 cut(s) 382, 428, 436, 644, 678
BstMWI GCNNNNNNNGC 2 cut(s) 10, 224
BstNI CCWGG 1 cut(s) 469
BstNSI RCATGY 1 cut(s) 33
BstSCI CCNGG 3 cut(s) 123, 467, 658
BstSFI CTRYAG 1 cut(s) 319
BstV1I GCAGC 2 cut(s) 74, 416
BstX2I RGATCY 1 cut(s) 644
BstXI CCANNNNNNTGG 1 cut(s) 372
BstYI RGATCY 1 cut(s) 644
BsuRI GGCC 4 cut(s) 136, 480, 519, 841
BtgI CCRYGG 1 cut(s) 624
BtgZI GCGATG 1 cut(s) 757
BtsCI GGATG 1 cut(s) 301
BtsIMutI CAGTG 1 cut(s) 502
Cac8I GCNNGC 6 cut(s) 6, 31, 521, 525, 539, 709
CaiI CAGNNNCTG 1 cut(s) 209
Cfr10I RCCGGY 3 cut(s) 4, 533, 762
Cfr13I GGNCC 4 cut(s) 145, 518, 602, 633
CseI GACGC 1 cut(s) 541
Csp6I GTAC 1 cut(s) 721
CviAII CATG 3 cut(s) 26, 30, 837
CviQI GTAC 1 cut(s) 721
DdeI CTNAG 1 cut(s) 819
DpnI GATC 5 cut(s) 384, 430, 438, 646, 680
DpnII GATC 5 cut(s) 382, 428, 436, 644, 678
DriI GACNNNNNGTC 1 cut(s) 491
EaeI YGGCCR 2 cut(s) 478, 839
Eam1105I GACNNNNNGTC 1 cut(s) 491
EciI GGCGGA 1 cut(s) 158
Ecl136II GAGCTC 1 cut(s) 541
Eco147I AGGCCT 1 cut(s) 136
Eco24I GRGCYC 2 cut(s) 543, 752
Eco47I GGWCC 3 cut(s) 145, 602, 633
Eco53kI GAGCTC 1 cut(s) 541
Eco88I CYCGRG 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 541
EcoRI GAATTC 1 cut(s) 734
EcoRII CCWGG 1 cut(s) 467
EcoT22I ATGCAT 1 cut(s) 31
EcoT38I GRGCYC 2 cut(s) 543, 752
FaeI CATG 3 cut(s) 29, 33, 840
FalI AAGNNNNNCTT 2 cut(s) 842, 874
FaqI GGGAC 1 cut(s) 214
FatI CATG 3 cut(s) 25, 29, 836
FauI CCCGC 2 cut(s) 528, 532
Fnu4HI GCNGC 5 cut(s) 88, 179, 182, 405, 481
FokI GGATG 1 cut(s) 308
FriOI GRGCYC 2 cut(s) 543, 752
Fsp4HI GCNGC 5 cut(s) 88, 179, 182, 405, 481
GluI GCNGC 5 cut(s) 88, 179, 182, 405, 481
GsaI CCCAGC 1 cut(s) 368
HaeIII GGCC 4 cut(s) 136, 480, 519, 841
HgaI GACGC 1 cut(s) 541
Hin1II CATG 3 cut(s) 29, 33, 840
HinfI GANTC 5 cut(s) 240, 283, 683, 830, 875
HphI GGTGA 3 cut(s) 124, 752, 880
Hpy166II GTNNAC 1 cut(s) 500
Hpy188I TCNGA 3 cut(s) 239, 740, 856
Hpy188III TCNNGA 2 cut(s) 575, 894
Hpy8I GTNNAC 1 cut(s) 500
Hpy99I CGWCG 1 cut(s) 489
HpyAV CCTTC 1 cut(s) 338
HpyCH4III ACNGT 3 cut(s) 78, 323, 637
HpyCH4V TGCA 4 cut(s) 29, 33, 330, 707
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 224
HpyF3I CTNAG 1 cut(s) 819
Hsp92II CATG 3 cut(s) 29, 33, 840
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 5 cut(s) 382, 428, 436, 644, 678
LmnI GCTCC 1 cut(s) 577
Lsp1109I GCAGC 2 cut(s) 74, 416
LweI GCATC 1 cut(s) 672
MaeIII GTNAC 2 cut(s) 241, 637
MalI GATC 5 cut(s) 384, 430, 438, 646, 680
MboI GATC 5 cut(s) 382, 428, 436, 644, 678
MboII GAAGA 2 cut(s) 179, 245
MflI RGATCY 1 cut(s) 644
MhlI GDGCHC 2 cut(s) 543, 752
MluCI AATT 7 cut(s) 149, 353, 419, 585, 689, 734, 867
MlyI GAGTC 2 cut(s) 249, 292
MmeI TCCRAC 1 cut(s) 358
Mph1103I ATGCAT 1 cut(s) 31
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 3 cut(s) 288, 717, 813
MspCI CTTAAG 1 cut(s) 287
MspR9I CCNGG 3 cut(s) 125, 469, 660
MvaI CCWGG 1 cut(s) 469
MwoI GCNNNNNNNGC 2 cut(s) 10, 224
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 2 cut(s) 125, 660
NdeII GATC 5 cut(s) 382, 428, 436, 644, 678
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 3 cut(s) 29, 33, 840
NlaIV GGNNCC 2 cut(s) 646, 825
NmuCI GTSAC 2 cut(s) 241, 637
NsiI ATGCAT 1 cut(s) 31
NspI RCATGY 1 cut(s) 33
PaeI GCATGC 1 cut(s) 33
PceI AGGCCT 1 cut(s) 136
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 3 cut(s) 683, 830, 875
PfoI TCCNGGA 1 cut(s) 467
PkrI GCNGC 5 cut(s) 89, 180, 183, 406, 482
PleI GAGTC 2 cut(s) 248, 291
PpsI GAGTC 2 cut(s) 248, 291
Psp124BI GAGCTC 1 cut(s) 543
Psp6I CCWGG 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 364
PspGI CCWGG 1 cut(s) 467
PspN4I GGNNCC 2 cut(s) 646, 825
PspPI GGNCC 4 cut(s) 145, 518, 602, 633
PsrI GAACNNNNNNTAC 2 cut(s) 771, 803
PstNI CAGNNNCTG 1 cut(s) 209
PsuI RGATCY 1 cut(s) 644
RsaI GTAC 1 cut(s) 722
RsaNI GTAC 1 cut(s) 721
SacI GAGCTC 1 cut(s) 543
SaqAI TTAA 3 cut(s) 288, 717, 813
SatI GCNGC 5 cut(s) 88, 179, 182, 405, 481
Sau3AI GATC 5 cut(s) 382, 428, 436, 644, 678
Sau96I GGNCC 4 cut(s) 145, 518, 602, 633
ScaI AGTACT 1 cut(s) 722
SchI GAGTC 2 cut(s) 249, 292
ScrFI CCNGG 3 cut(s) 125, 469, 660
SduI GDGCHC 2 cut(s) 543, 752
SetI ASST 9 cut(s) 54, 114, 208, 294, 329, 543, 615, 732, 893
SfaNI GCATC 1 cut(s) 672
SfcI CTRYAG 1 cut(s) 319
SgrAI CRCCGGYG 1 cut(s) 533
SinI GGWCC 3 cut(s) 145, 602, 633
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SphI GCATGC 1 cut(s) 33
Sse9I AATT 7 cut(s) 149, 353, 419, 585, 689, 734, 867
SseBI AGGCCT 1 cut(s) 136
SsiI CCGC 6 cut(s) 143, 178, 181, 481, 521, 525
SstI GAGCTC 1 cut(s) 543
StuI AGGCCT 1 cut(s) 136
StyD4I CCNGG 3 cut(s) 123, 467, 658
TaaI ACNGT 3 cut(s) 78, 323, 637
TaqI TCGA 2 cut(s) 400, 681
TasI AATT 7 cut(s) 149, 353, 419, 585, 689, 734, 867
TatI WGTACW 1 cut(s) 720
TauI GCSGC 3 cut(s) 181, 184, 483
TfiI GAWTC 3 cut(s) 683, 830, 875
Tru1I TTAA 3 cut(s) 288, 717, 813
Tru9I TTAA 3 cut(s) 288, 717, 813
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 2 cut(s) 241, 637
TseI GCWGC 2 cut(s) 87, 404
Tsp45I GTSAC 2 cut(s) 241, 637
TspDTI ATGAA 2 cut(s) 14, 357
TspRI CASTG 1 cut(s) 502
Vha464I CTTAAG 1 cut(s) 287
VpaK11BI GGWCC 3 cut(s) 145, 602, 633
XapI RAATTY 3 cut(s) 353, 419, 734
XceI RCATGY 1 cut(s) 33
ZrmI AGTACT 1 cut(s) 722
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.