Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011571

Physical Location & Seq

Genomic Coordinates Forward (+)

30647846 .. 30652437

Gene Structure 4592 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1231500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2001 bp

ATGAGGCCGGTGTATGTCAGGCAGAAGAGCAATAGTAGTGGCGGCACCCCGGGGGCACCAACTTCGCCAATGATGTCGCCAATGAACCACCATGGGAGGTCAGGGTCGGTTGGAATGGTGGGTGCCAGGAAAGTGCAGCACACGAAAGCCGCAGCGCAAAGGCTTGCACATGTGATGGCTCACAAACCAGCTGACGACGACGGTGAGGATGATGACGATCTTTCTTATGATCCTGGCTTGTCAAGCCGCACAGGCAGCCTTGGACTTGTCGGCGGAAGATCAATGCGCCCACGGTCTCCAATGTCAGTTCGTGCCGCTCAAGAACATTCTACATCTACACGGGGACGATCATCACCGTCTCTTAACTCCATGGAACTACCATCTTCTACCCATTCAACACCATCTAGTCGACCATATCAGTACGCAGAACAACCGTCATCAGCTCATTCAACACCAGCAAGCCGAACATACCAATCCATCAACACAAAACCCATTAACCCTGTTGTCGAACAACCACATTCTCTTCGCTCTTCAATCGCTACTAGACCATCCCAGCCTTCGAATTCCAACGAACAACCTACCTCTGCTCGTACTATATCGGGCATGAACTCTGCTCGATCATCTCAGCCAACAAATGTTGTCGAACAACCTACCTCTGCTCGTTCTATTTCAGGCCTGAACTCTGCCCGGTCATCTCAGGCTACCAGTGCTGTCGAACAACCTACCTCTGCACGCTCTTTAGCTGTTGCCCGTCCGCAGTTAGGAACCAAGCCAGTCCACATCATGCCAGCTAGTGTACCTATATCACTTAGGCCACCCTCTTCTGCTGATCCTCCAGCTGATAATCGGAGAGAGCCAGCACCTATACCACTACGGCCACCATCTACTGCCGGTGGATCAGACGCTTCAGCTGATACTCGGAGAGATAAAAGGATGTCTTTGGATTTAGGGACTTTTAACATGAGAGAAAATCGGGCGCAGCGTTCTTCTGCTTTAGAAGATGAGCTTGATATGCTCCAGGAAGAAAATGAAAATTTACTCGAAAAGCTTCGAGTTGCAGAAGAGAGGTGCGACGAAACAGAAGCAAGAGCTCGACAGCTCGAGCAACAGGTTGCTAATCTTGGCGAAGGTGTGACGTTGGAAGCCCGTCTTCTAAGCAGGCAAGCTTCAAAGATTTCATGCGCTGCTTTGAGAGAGGCTGCACAGACTCATGGTGGTAGCGCTCATGGTGGTAGAAATGACGAAGCAGAGTACGAACTCAGATCGCTTCGGATTACGACTCAACGAATGATACTGAGTCGCGAAGAGATGGAAGAGGTGGTCTTGAAGAGATCTTGGCTAGCTCGGTACTGGAGTTTATGCGTTGAGCATGGTATTCATGCTGAGATAGCGGCATCAAGATCCGAATTCTGGTCATCGCTTGCTCCTCTTCCTGTTCAAGCTATATTAGAAGCCGGACAAAAAGCGAAAGAGGGGATGGACAACAATGATCTAGATGAAAGAGAAGATAGTCCACGAGATTTGAATGAACTCTTGGGCGAGAGAAACATTGAGAGCATGCTCTTGGTGGAGAAGGGTCTGCGCGAACTTGCTTCGTTGAAGGTAGAGGAAGCAGTGGCACTTGCAATGGCTAAACATCGACGCCCTAGTTCAAAGAAAACTGGTCTTATAGATTTGAAGCTACCAATTGATGGACAGTTTGAAGCATTTGAACTCAGCAAAGAGGAGTCTGAAGATGTGCTTTTCAAGCAGGCTTGGCTTGCATATTTCTGGAGAAGAGCTACAAACCATGGCGTGGAACCAGATATAGCAGATGAGCGTTTGCAATTCTGGATCAACCACAACTCCAAGTCACCCGCCACCTCGCACGATGCAGTTGATATTGAGCGCGGTCTTCTGGAGCTGAAGAAGCTGGGAATCGAAACCCAGTTATGGAGAGAATCTCGGAAATGGCTCGAGCATGATTCGATGCATAGAACGCAAAGTCTGTACGACTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

667

Amino Acids

73.46

Weight (kDa)

6.5

Isoelectric Point (pI)

64.78

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011571)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g04230
malus_domestica	MD00G1051700.v1.1 MD12G1231500.v1.1
prunus_persica	Prupe.6G335100_v2.0.a1
pyrus_communis	pycom04g19150 pycom12g21430
rosa_chinensis	RchiOBHm_Chr3g0452891
rosa_laevigata	RLG00000025562
rosa_multiflora	Rmu_sc0002823.1_g000008
rosa_roxburghii	Rroxscaffold_6G00429900
rosa_rugosa	Rorug02G0643400 Rorug02G0643500 Rorug02G0643500 Rorug02G0643500 Rorug02G0643500
rosa_samantha	Rh3AG046200 Rh3BG048700 Rh3CG047300 Rh3DG048300
rosa_wichuraiana	Rw3G003630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	44, 55, 122
AccB7I	CCANNNNNTGG	2 cut(s)	1691, 1795
AccBSI	CCGCTC	1 cut(s)	317
AccI	GTMKAC	1 cut(s)	409
AccII	CGCG	3 cut(s)	1302, 1584, 1890
AciI	CCGC	9 cut(s)	42, 150, 247, 273, 315, 755, 1391, 1857, 1890
AclWI	GGATC	5 cut(s)	224, 824, 904, 1395, 1841
AcoI	YGGCCR	1 cut(s)	875
AcsI	RAATTY	3 cut(s)	562, 1033, 1406
AcuI	CTGAAG	3 cut(s)	891, 1752, 1925
AcyI	GRCGYC	1 cut(s)	1642
AfaI	GTAC	6 cut(s)	422, 592, 798, 1253, 1349, 1991
AfeI	AGCGCT	1 cut(s)	1222
AfiI	CCNNNNNNNGG	5 cut(s)	761, 1410, 1691, 1795, 1932
AflIII	ACRYGT	1 cut(s)	169
AjnI	CCWGG	3 cut(s)	125, 232, 1017
AjuI	GAANNNNNNNTTGG	2 cut(s)	1517, 1549
Alw21I	GWGCWC	1 cut(s)	1093
Alw26I	GTCTC	2 cut(s)	300, 363
AlwI	GGATC	5 cut(s)	224, 824, 904, 1395, 1841
Ama87I	CYCGRG	3 cut(s)	49, 1100, 1955
Aor51HI	AGCGCT	1 cut(s)	1222
AoxI	GGCC	4 cut(s)	5, 673, 812, 875
ApeKI	GCWGC	6 cut(s)	136, 152, 255, 979, 1184, 1199
ApoI	RAATTY	3 cut(s)	562, 1033, 1406
Asp700I	GAANNNNTTC	1 cut(s)	1047
AspLEI	GCGC	7 cut(s)	157, 288, 979, 1184, 1223, 1584, 1890
AsuC2I	CCSGG	3 cut(s)	50, 51, 688
AsuHPI	GGTGA	3 cut(s)	215, 345, 1845
AsuII	TTCGAA	1 cut(s)	560
AsuNHI	GCTAGC	1 cut(s)	1339
AvaI	CYCGRG	3 cut(s)	49, 1100, 1955
BaeGI	GKGCMC	1 cut(s)	58
BaeI	ACNNNNGTAYC	2 cut(s)	1339, 1372
BanI	GGYRCC	3 cut(s)	44, 55, 122
BanII	GRGCYC	1 cut(s)	1093
BauI	CACGAG	1 cut(s)	1515
BbsI	GAAGAC	2 cut(s)	1142, 1886
Bbv12I	GWGCWC	1 cut(s)	1093
BbvI	GCAGC	6 cut(s)	148, 164, 267, 991, 1171, 1186
BccI	CCATC	9 cut(s)	169, 388, 409, 485, 556, 889, 1303, 1471, 1685
BceAI	ACGGC	1 cut(s)	890
BcgI	CGANNNNNNTGC	2 cut(s)	45, 79
BciT130I	CCWGG	3 cut(s)	127, 234, 1019
BcnI	CCSGG	3 cut(s)	50, 51, 688
BcoDI	GTCTC	2 cut(s)	300, 363
BfaI	CTAG	6 cut(s)	405, 543, 792, 1340, 1493, 1647
BfoI	RGCGCY	1 cut(s)	1224
BglI	GCCNNNNNGGC	1 cut(s)	252
BglII	AGATCT	1 cut(s)	1331
Bme1390I	CCNGG	6 cut(s)	50, 51, 127, 234, 688, 1019
BmeT110I	CYCGRG	3 cut(s)	49, 1100, 1955
BmiI	GGNNCC	5 cut(s)	46, 57, 124, 766, 1800
BmrFI	CCNGG	6 cut(s)	50, 51, 127, 234, 688, 1019
BmrI	ACTGGG	1 cut(s)	1921
BmsI	GCATC	3 cut(s)	1403, 1861, 1959
BmtI	GCTAGC	1 cut(s)	1343
BmuI	ACTGGG	1 cut(s)	1921
BpiI	GAAGAC	2 cut(s)	1142, 1886
BplI	GAGNNNNNCTC	2 cut(s)	1927, 1959
BpmI	CTGGAG	5 cut(s)	819, 1001, 1372, 1792, 1919
Bpu14I	TTCGAA	1 cut(s)	560
BpuEI	CTTGAG	1 cut(s)	303
BpuMI	CCSGG	3 cut(s)	50, 51, 688
BsaBI	GATNNNNATC	1 cut(s)	216
BsaHI	GRCGYC	1 cut(s)	1642
BsaI	GGTCTC	1 cut(s)	300
BsaJI	CCNNGG	8 cut(s)	48, 49, 50, 91, 259, 290, 369, 1789
BsaXI	ACNNNNNCTCC	2 cut(s)	1829, 1859
Bsc4I	CCNNNNNNNGG	5 cut(s)	761, 1410, 1691, 1795, 1932
Bse118I	RCCGGY	2 cut(s)	7, 890
Bse1I	ACTGG	5 cut(s)	705, 773, 1355, 1666, 1927
Bse3DI	GCAATG	1 cut(s)	1632
Bse8I	GATNNNNATC	1 cut(s)	216
BseBI	CCWGG	3 cut(s)	127, 234, 1019
BseDI	CCNNGG	8 cut(s)	48, 49, 50, 91, 259, 290, 369, 1789
BseGI	GGATG	4 cut(s)	214, 548, 939, 1482
BseJI	GATNNNNATC	1 cut(s)	216
BseLI	CCNNNNNNNGG	5 cut(s)	761, 1410, 1691, 1795, 1932
BseMI	GCAATG	1 cut(s)	1632
BseMII	CTCAG	6 cut(s)	638, 710, 1273, 1286, 1374, 1729
BseNI	ACTGG	5 cut(s)	705, 773, 1355, 1666, 1927
BseRI	GAGGAG	2 cut(s)	1416, 1739
BseSI	GKGCMC	1 cut(s)	58
BseXI	GCAGC	6 cut(s)	148, 164, 267, 991, 1171, 1186
BseYI	CCCAGC	2 cut(s)	552, 1912
BsgI	GTGCAG	3 cut(s)	155, 714, 1185
Bsh1236I	CGCG	3 cut(s)	1302, 1584, 1890
BshFI	GGCC	4 cut(s)	7, 675, 814, 877
BshNI	GGYRCC	3 cut(s)	44, 55, 122
BsiHKAI	GWGCWC	1 cut(s)	1093
BsiHKCI	CYCGRG	3 cut(s)	49, 1100, 1955
BsiSI	CCGG	5 cut(s)	8, 50, 688, 891, 1455
BslFI	GGGAC	2 cut(s)	357, 964
BslI	CCNNNNNNNGG	5 cut(s)	761, 1410, 1691, 1795, 1932
BsmAI	GTCTC	2 cut(s)	300, 363
BsmBI	CGTCTC	1 cut(s)	363
BsmFI	GGGAC	2 cut(s)	357, 964
BsnI	GGCC	4 cut(s)	7, 675, 814, 877
Bso31I	GGTCTC	1 cut(s)	300
BsoBI	CYCGRG	3 cut(s)	49, 1100, 1955
Bsp119I	TTCGAA	1 cut(s)	560
Bsp1286I	GDGCHC	2 cut(s)	58, 1093
Bsp19I	CCATGG	3 cut(s)	91, 369, 1789
Bsp68I	TCGCGA	1 cut(s)	1302
BspACI	CCGC	9 cut(s)	42, 150, 247, 273, 315, 755, 1391, 1857, 1890
BspANI	GGCC	4 cut(s)	7, 675, 814, 877
BspCNI	CTCAG	6 cut(s)	637, 709, 1272, 1287, 1375, 1728
BspFNI	CGCG	3 cut(s)	1302, 1584, 1890
BspLI	GGNNCC	5 cut(s)	46, 57, 124, 766, 1800
BspOI	GCTAGC	1 cut(s)	1343
BspPI	GGATC	5 cut(s)	224, 824, 904, 1395, 1841
BspQI	GCTCTTC	3 cut(s)	20, 535, 1771
BspT104I	TTCGAA	1 cut(s)	560
BspT107I	GGYRCC	3 cut(s)	44, 55, 122
BspTNI	GGTCTC	1 cut(s)	300
BsrBI	CCGCTC	1 cut(s)	317
BsrDI	GCAATG	1 cut(s)	1632
BsrFI	RCCGGY	2 cut(s)	7, 890
BsrI	ACTGG	5 cut(s)	705, 773, 1355, 1666, 1927
BssAI	RCCGGY	2 cut(s)	7, 890
BssECI	CCNNGG	8 cut(s)	48, 49, 50, 91, 259, 290, 369, 1789
BssNI	GRCGYC	1 cut(s)	1642
BssSI	CACGAG	1 cut(s)	1515
BssT1I	CCWWGG	4 cut(s)	91, 259, 369, 1789
Bst2BI	CACGAG	1 cut(s)	1515
Bst2UI	CCWGG	3 cut(s)	127, 234, 1019
Bst4CI	ACNGT	5 cut(s)	203, 294, 357, 435, 1698
BstACI	GRCGYC	1 cut(s)	1642
BstBI	TTCGAA	1 cut(s)	560
BstDEI	CTNAG	8 cut(s)	624, 696, 809, 1154, 1259, 1295, 1383, 1715
BstDSI	CCRYGG	4 cut(s)	91, 290, 369, 1789
BstF5I	GGATG	4 cut(s)	214, 548, 939, 1482
BstFNI	CGCG	3 cut(s)	1302, 1584, 1890
BstH2I	RGCGCY	1 cut(s)	1224
BstHHI	GCGC	7 cut(s)	157, 288, 979, 1184, 1223, 1584, 1890
BstMAI	GTCTC	2 cut(s)	300, 363
BstNI	CCWGG	3 cut(s)	127, 234, 1019
BstNSI	RCATGY	2 cut(s)	173, 1561
BstSCI	CCNGG	6 cut(s)	48, 49, 125, 232, 686, 1017
BstSLI	GKGCMC	1 cut(s)	58
BstUI	CGCG	3 cut(s)	1302, 1584, 1890
BstV1I	GCAGC	6 cut(s)	148, 164, 267, 991, 1171, 1186
BstV2I	GAAGAC	2 cut(s)	1142, 1886
BstX2I	RGATCY	2 cut(s)	1331, 1400
BstYI	RGATCY	2 cut(s)	1331, 1400
BsuRI	GGCC	4 cut(s)	7, 675, 814, 877
BtgI	CCRYGG	4 cut(s)	91, 290, 369, 1789
BtgZI	GCGATG	1 cut(s)	1401
BtsCI	GGATG	4 cut(s)	214, 548, 939, 1482
BtsI	GCAGTG	1 cut(s)	1620
BtsIMutI	CAGTG	2 cut(s)	712, 1620
BtuMI	TCGCGA	1 cut(s)	1302
CfoI	GCGC	7 cut(s)	157, 288, 979, 1184, 1223, 1584, 1890
Cfr10I	RCCGGY	2 cut(s)	7, 890
Cfr9I	CCCGGG	1 cut(s)	49
CseI	GACGC	2 cut(s)	911, 1650
Csp6I	GTAC	6 cut(s)	421, 591, 797, 1252, 1348, 1990
CspCI	CAANNNNNGTGG	4 cut(s)	19, 54, 1503, 1538
CviQI	GTAC	6 cut(s)	421, 591, 797, 1252, 1348, 1990
DdeI	CTNAG	8 cut(s)	624, 696, 809, 1154, 1259, 1295, 1383, 1715
EaeI	YGGCCR	1 cut(s)	875
EciI	GGCGGA	1 cut(s)	288
Ecl136II	GAGCTC	1 cut(s)	1091
Eco130I	CCWWGG	4 cut(s)	91, 259, 369, 1789
Eco147I	AGGCCT	1 cut(s)	675
Eco24I	GRGCYC	1 cut(s)	1093
Eco31I	GGTCTC	1 cut(s)	300
Eco47III	AGCGCT	1 cut(s)	1222
Eco53kI	GAGCTC	1 cut(s)	1091
Eco57I	CTGAAG	3 cut(s)	891, 1752, 1925
Eco88I	CYCGRG	3 cut(s)	49, 1100, 1955
EcoICRI	GAGCTC	1 cut(s)	1091
EcoRI	GAATTC	2 cut(s)	562, 1406
EcoRII	CCWGG	3 cut(s)	125, 232, 1017
EcoT14I	CCWWGG	4 cut(s)	91, 259, 369, 1789
EcoT22I	ATGCAT	1 cut(s)	1974
EcoT38I	GRGCYC	1 cut(s)	1093
ErhI	CCWWGG	4 cut(s)	91, 259, 369, 1789
Esp3I	CGTCTC	1 cut(s)	363
FalI	AAGNNNNNCTT	8 cut(s)	922, 954, 990, 1022, 1134, 1166, 1725, 1757
FaqI	GGGAC	2 cut(s)	357, 964
FauI	CCCGC	1 cut(s)	1864
FblI	GTMKAC	1 cut(s)	409
FokI	GGATG	4 cut(s)	221, 535, 946, 1489
FriOI	GRGCYC	1 cut(s)	1093
FspBI	CTAG	6 cut(s)	405, 543, 792, 1340, 1493, 1647
GlaI	GCGC	7 cut(s)	156, 287, 978, 1183, 1222, 1583, 1889
GsaI	CCCAGC	2 cut(s)	556, 1916
GsuI	CTGGAG	5 cut(s)	819, 1001, 1372, 1792, 1919
HaeII	RGCGCY	1 cut(s)	1224
HaeIII	GGCC	4 cut(s)	7, 675, 814, 877
HapII	CCGG	5 cut(s)	8, 50, 688, 891, 1455
HgaI	GACGC	2 cut(s)	911, 1650
HhaI	GCGC	7 cut(s)	157, 288, 979, 1184, 1223, 1584, 1890
Hin1I	GRCGYC	1 cut(s)	1642
Hin6I	GCGC	7 cut(s)	155, 286, 977, 1182, 1221, 1582, 1888
HinP1I	GCGC	7 cut(s)	155, 286, 977, 1182, 1221, 1582, 1888
HincII	GTYRAC	1 cut(s)	410
HindII	GTYRAC	1 cut(s)	410
HindIII	AAGCTT	2 cut(s)	1046, 1164
HinfI	GANTC	7 cut(s)	1207, 1279, 1297, 1727, 1917, 1940, 1964
HpaII	CCGG	5 cut(s)	8, 50, 688, 891, 1455
HphI	GGTGA	3 cut(s)	215, 345, 1845
Hpy166II	GTNNAC	4 cut(s)	410, 778, 797, 1514
Hpy188I	TCNGA	9 cut(s)	849, 901, 921, 1262, 1272, 1405, 1732, 1947, 2000
Hpy188III	TCNNGA	8 cut(s)	320, 1301, 1324, 1398, 1493, 1771, 1831, 1898
Hpy8I	GTNNAC	4 cut(s)	410, 778, 797, 1514
Hpy99I	CGWCG	4 cut(s)	200, 203, 1076, 1644
HpyAV	CCTTC	4 cut(s)	567, 1121, 1567, 1594
HpyCH4III	ACNGT	5 cut(s)	203, 294, 357, 435, 1698
HpyCH4IV	ACGT	1 cut(s)	1136
HpyF3I	CTNAG	8 cut(s)	624, 696, 809, 1154, 1259, 1295, 1383, 1715
HpySE526I	ACGT	1 cut(s)	1136
Hsp92I	GRCGYC	1 cut(s)	1642
HspAI	GCGC	7 cut(s)	155, 286, 977, 1182, 1221, 1582, 1888
LguI	GCTCTTC	3 cut(s)	20, 535, 1771
LmnI	GCTCC	3 cut(s)	1020, 1429, 1900
Lsp1109I	GCAGC	6 cut(s)	148, 164, 267, 991, 1171, 1186
LweI	GCATC	3 cut(s)	1403, 1861, 1959
MaeI	CTAG	6 cut(s)	405, 543, 792, 1340, 1493, 1647
MaeII	ACGT	1 cut(s)	1136
MaeIII	GTNAC	2 cut(s)	1132, 1851
MbiI	CCGCTC	1 cut(s)	317
MfeI	CAATTG	1 cut(s)	1686
MflI	RGATCY	2 cut(s)	1331, 1400
MhlI	GDGCHC	2 cut(s)	58, 1093
MluCI	AATT	5 cut(s)	562, 1033, 1406, 1686, 1826
MlyI	GAGTC	4 cut(s)	1201, 1273, 1306, 1736
MmeI	TCCRAC	3 cut(s)	91, 591, 1119
Mph1103I	ATGCAT	1 cut(s)	1974
MroXI	GAANNNNTTC	1 cut(s)	1047
MseI	TTAA	3 cut(s)	363, 495, 957
MspA1I	CMGCKG	3 cut(s)	191, 839, 911
MspI	CCGG	5 cut(s)	8, 50, 688, 891, 1455
MspR9I	CCNGG	6 cut(s)	50, 51, 127, 234, 688, 1019
MunI	CAATTG	1 cut(s)	1686
MvaI	CCWGG	3 cut(s)	127, 234, 1019
MvnI	CGCG	3 cut(s)	1302, 1584, 1890
NciI	CCSGG	3 cut(s)	50, 51, 688
NcoI	CCATGG	3 cut(s)	91, 369, 1789
NheI	GCTAGC	1 cut(s)	1339
NlaIV	GGNNCC	5 cut(s)	46, 57, 124, 766, 1800
NmuCI	GTSAC	2 cut(s)	1132, 1851
NruI	TCGCGA	1 cut(s)	1302
NsiI	ATGCAT	1 cut(s)	1974
NspI	RCATGY	2 cut(s)	173, 1561
NspV	TTCGAA	1 cut(s)	560
PaeI	GCATGC	1 cut(s)	1561
PaeR7I	CTCGAG	2 cut(s)	1100, 1955
PceI	AGGCCT	1 cut(s)	675
PciI	ACATGT	1 cut(s)	169
PciSI	GCTCTTC	3 cut(s)	20, 535, 1771
PcsI	WCGNNNNNNNCGW	1 cut(s)	979
PdmI	GAANNNNTTC	1 cut(s)	1047
PfeI	GAWTC	3 cut(s)	1917, 1940, 1964
PflMI	CCANNNNNTGG	2 cut(s)	1691, 1795
PfoI	TCCNGGA	1 cut(s)	1017
PleI	GAGTC	4 cut(s)	1201, 1273, 1305, 1735
PpsI	GAGTC	4 cut(s)	1201, 1273, 1305, 1735
PscI	ACATGT	1 cut(s)	169
Psp124BI	GAGCTC	1 cut(s)	1093
Psp6I	CCWGG	3 cut(s)	125, 232, 1017
PspFI	CCCAGC	2 cut(s)	552, 1912
PspGI	CCWGG	3 cut(s)	125, 232, 1017
PspN4I	GGNNCC	5 cut(s)	46, 57, 124, 766, 1800
PspXI	VCTCGAGB	2 cut(s)	1100, 1955
PsuI	RGATCY	2 cut(s)	1331, 1400
PvuII	CAGCTG	3 cut(s)	191, 839, 911
RruI	TCGCGA	1 cut(s)	1302
RsaI	GTAC	6 cut(s)	422, 592, 798, 1253, 1349, 1991
RsaNI	GTAC	6 cut(s)	421, 591, 797, 1252, 1348, 1990
SacI	GAGCTC	1 cut(s)	1093
SalI	GTCGAC	1 cut(s)	408
SapI	GCTCTTC	3 cut(s)	20, 535, 1771
SaqAI	TTAA	3 cut(s)	363, 495, 957
SchI	GAGTC	4 cut(s)	1201, 1273, 1306, 1736
ScrFI	CCNGG	6 cut(s)	50, 51, 127, 234, 688, 1019
SduI	GDGCHC	2 cut(s)	58, 1093
SfaNI	GCATC	3 cut(s)	1403, 1861, 1959
Sfr274I	CTCGAG	2 cut(s)	1100, 1955
SfuI	TTCGAA	1 cut(s)	560
SlaI	CTCGAG	2 cut(s)	1100, 1955
SmaI	CCCGGG	1 cut(s)	51
SmlI	CTYRAG	3 cut(s)	318, 1100, 1955
SmoI	CTYRAG	3 cut(s)	318, 1100, 1955
SphI	GCATGC	1 cut(s)	1561
Sse9I	AATT	5 cut(s)	562, 1033, 1406, 1686, 1826
SseBI	AGGCCT	1 cut(s)	675
SsiI	CCGC	9 cut(s)	42, 150, 247, 273, 315, 755, 1391, 1857, 1890
SspMI	CTAG	6 cut(s)	405, 543, 792, 1340, 1493, 1647
SstI	GAGCTC	1 cut(s)	1093
StuI	AGGCCT	1 cut(s)	675
StyD4I	CCNGG	6 cut(s)	48, 49, 125, 232, 686, 1017
StyI	CCWWGG	4 cut(s)	91, 259, 369, 1789
TaaI	ACNGT	5 cut(s)	203, 294, 357, 435, 1698
TaiI	ACGT	1 cut(s)	1139
TasI	AATT	5 cut(s)	562, 1033, 1406, 1686, 1826
TauI	GCSGC	5 cut(s)	45, 152, 249, 317, 1394
TfiI	GAWTC	3 cut(s)	1917, 1940, 1964
Tru1I	TTAA	3 cut(s)	363, 495, 957
Tru9I	TTAA	3 cut(s)	363, 495, 957
TscAI	CASTG	2 cut(s)	712, 1620
TseFI	GTSAC	2 cut(s)	1132, 1851
TseI	GCWGC	6 cut(s)	136, 152, 255, 979, 1184, 1199
Tsp45I	GTSAC	2 cut(s)	1132, 1851
TspDTI	ATGAA	7 cut(s)	98, 620, 1044, 1167, 1367, 1512, 1542
TspMI	CCCGGG	1 cut(s)	49
TspRI	CASTG	2 cut(s)	712, 1620
Van91I	CCANNNNNTGG	2 cut(s)	1691, 1795
XapI	RAATTY	3 cut(s)	562, 1033, 1406
XbaI	TCTAGA	1 cut(s)	1492
XceI	RCATGY	2 cut(s)	173, 1561
XhoI	CTCGAG	2 cut(s)	1100, 1955
XmaI	CCCGGG	1 cut(s)	49
XmiI	GTMKAC	1 cut(s)	409
XmnI	GAANNNNTTC	1 cut(s)	1047
XspI	CTAG	6 cut(s)	405, 543, 792, 1340, 1493, 1647
Zsp2I	ATGCAT	1 cut(s)	1974

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.