MD12G1234200.v1.1

SEC-C motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011179
Physical Location & Seq
Forward (+)
30959962 .. 30962695
2734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1234200.v1.1.491

Sequence Viewer

Length: 684 bp
ATGCATCGCTCTTCGCGAATCATAAGCACCACATTCTGCAACAAAAACTCGAAGTTCCCGTCATCTTTCTTCGGCTACCGTTACGGTGACCGCAACCTCGCTCCGTCGTTTCACCGCCCGATCTCCTCCACGACGCACCTGGATAACTCATGGATGAACAAGATCAAAGGGGTCTTCACCGGCAACAAGGCCTCTCCGGAGGATGCAAAGTCCAGCTCCGATTCGTTCACGCTGCTTCGATTTGCGGATGAGATGAAGAATGCTAGAAGAATTGGGGCGTTCAAGCAATACATTGTGGGGAGAAGCAGTGAAGCTACTTTTGCCGATGCTTTCGAGAAGCAGGAAGCGATTATTCGCTATCTCGGAGGATTTGATTCCACTGGAGAGAATCTCCAAGTTGCTCAAAAACAAGAAGCAGCGAAGCATTGTAAATGCACGATTGCTGATGTTGAGAATGCTCTTGCAAAATTCACGTGGGCTAGAGAAGCACAAAAGAAGATGGAGAATTTAAAGAAAGAAGGGAAACCAATGCCAAGGAACATTAACGAGGTGCAAAAATTGATGGGTTCAACGCCATTGGACCTTGCCAAGTCTAATTTGGCTAAGAGTGGGCAAATCAGTAGGAATGCGCTCTGTCCATGTGGCTCCAAGAAGAGATACAAACGGTGTTGCGGCAAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEC-C

Protein Analysis

228

Amino Acids

25.44

Weight (kDa)

9.76

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEC-C PF02810 208 - 226 1.2e-06 SEC-C motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04950
fragaria_vesca FvH4_6g03930
malus_domestica MD12G1234200.v1.1
prunus_persica Prupe.6G337300_v2.0.a1
pyrus_communis pycom04g19330 pycom12g21660
rosa_chinensis RchiOBHm_Chr3g0452481 RchiOBHm_Chr3g0452521
rosa_laevigata RLG00000025595 RLG00000025598
rosa_multiflora Rmu_co8297771.1_g000001 Rmu_sc0011680.1_g000004
rosa_roxburghii Rroxscaffold_6G00429510
rosa_rugosa Rorug02G0641200
rosa_samantha Rh3AG043000 Rh3BG044700 Rh3CG043300 Rh3DG044100
rosa_wichuraiana Rw3G003330 Rw3G003360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 16
AccIII TCCGGA 1 cut(s) 196
AciI CCGC 4 cut(s) 91, 115, 245, 672
AcsI RAATTY 2 cut(s) 467, 505
AcvI CACGTG 1 cut(s) 474
AgsI TTSAA 2 cut(s) 283, 570
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 2 cut(s) 216, 314
AluI AGCT 2 cut(s) 216, 314
Aor13HI TCCGGA 1 cut(s) 196
AoxI GGCC 1 cut(s) 189
ApeKI GCWGC 2 cut(s) 232, 416
ApoI RAATTY 2 cut(s) 467, 505
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 1 cut(s) 631
AspS9I GGNCC 1 cut(s) 580
AsuHPI GGTGA 3 cut(s) 98, 104, 169
AvaII GGWCC 1 cut(s) 580
BbrPI CACGTG 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 219, 428
BccI CCATC 2 cut(s) 493, 556
BciT130I CCWGG 1 cut(s) 140
BfaI CTAG 2 cut(s) 264, 480
BisI GCNGC 3 cut(s) 233, 417, 673
BlsI GCNGC 3 cut(s) 234, 418, 674
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 1 cut(s) 580
BmgT120I GGNCC 1 cut(s) 580
BmiI GGNNCC 1 cut(s) 646
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 3 cut(s) 13, 193, 316
BpiI GAAGAC 1 cut(s) 166
BplI GAGNNNNNCTC 2 cut(s) 375, 407
BpmI CTGGAG 1 cut(s) 402
BsaAI YACGTR 1 cut(s) 474
BsaJI CCNNGG 1 cut(s) 533
BsaWI WCCGGW 1 cut(s) 196
BsaXI ACNNNNNCTCC 2 cut(s) 292, 322
Bse118I RCCGGY 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 385
BseAI TCCGGA 1 cut(s) 196
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 533
BseGI GGATG 3 cut(s) 159, 208, 253
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 1 cut(s) 115
BseXI GCAGC 2 cut(s) 219, 428
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 1 cut(s) 191
BsiSI CCGG 2 cut(s) 180, 197
BsmI GAATGC 3 cut(s) 265, 460, 631
BsnI GGCC 1 cut(s) 191
Bsp13I TCCGGA 1 cut(s) 196
Bsp143I GATC 2 cut(s) 120, 162
Bsp68I TCGCGA 1 cut(s) 16
BspACI CCGC 4 cut(s) 91, 115, 245, 672
BspANI GGCC 1 cut(s) 191
BspEI TCCGGA 1 cut(s) 196
BspFNI CGCG 1 cut(s) 16
BspLI GGNNCC 1 cut(s) 646
BspQI GCTCTTC 1 cut(s) 16
BsrFI RCCGGY 1 cut(s) 179
BsrI ACTGG 1 cut(s) 385
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 1 cut(s) 533
BssMI GATC 2 cut(s) 120, 162
BssT1I CCWWGG 1 cut(s) 533
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 3 cut(s) 80, 86, 666
Bst6I CTCTTC 2 cut(s) 16, 647
BstBAI YACGTR 1 cut(s) 474
BstDEI CTNAG 1 cut(s) 603
BstEII GGTNACC 1 cut(s) 86
BstF5I GGATG 3 cut(s) 159, 208, 253
BstFNI CGCG 1 cut(s) 16
BstHHI GCGC 1 cut(s) 631
BstKTI GATC 2 cut(s) 123, 165
BstMBI GATC 2 cut(s) 120, 162
BstMWI GCNNNNNNNGC 2 cut(s) 320, 485
BstNI CCWGG 1 cut(s) 140
BstPI GGTNACC 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 138
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 2 cut(s) 219, 428
BstV2I GAAGAC 1 cut(s) 166
BsuRI GGCC 1 cut(s) 191
BtsCI GGATG 3 cut(s) 159, 208, 253
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 2 cut(s) 313, 378
BtuMI TCGCGA 1 cut(s) 16
CfoI GCGC 1 cut(s) 631
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 580
CseI GACGC 1 cut(s) 142
CviAII CATG 2 cut(s) 150, 639
CviJI RGCY 7 cut(s) 75, 191, 216, 314, 479, 602, 645
CviKI_1 RGCY 7 cut(s) 75, 191, 216, 314, 479, 602, 645
DdeI CTNAG 1 cut(s) 603
DpnI GATC 2 cut(s) 122, 164
DpnII GATC 2 cut(s) 120, 162
DraI TTTAAA 1 cut(s) 510
Eam1104I CTCTTC 2 cut(s) 16, 647
EarI CTCTTC 2 cut(s) 16, 647
Eco130I CCWWGG 1 cut(s) 533
Eco147I AGGCCT 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 580
Eco72I CACGTG 1 cut(s) 474
Eco91I GGTNACC 1 cut(s) 86
EcoO65I GGTNACC 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 533
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 533
FaeI CATG 2 cut(s) 153, 642
FaiI YATR 3 cut(s) 23, 151, 640
FatI CATG 2 cut(s) 149, 638
Fnu4HI GCNGC 3 cut(s) 233, 417, 673
FokI GGATG 3 cut(s) 166, 215, 260
Fsp4HI GCNGC 3 cut(s) 233, 417, 673
FspBI CTAG 2 cut(s) 264, 480
GlaI GCGC 1 cut(s) 630
GluI GCNGC 3 cut(s) 233, 417, 673
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 1 cut(s) 191
HapII CCGG 2 cut(s) 180, 197
HgaI GACGC 1 cut(s) 142
HhaI GCGC 1 cut(s) 631
Hin1II CATG 2 cut(s) 153, 642
Hin6I GCGC 1 cut(s) 629
HinP1I GCGC 1 cut(s) 629
HinfI GANTC 4 cut(s) 18, 221, 374, 388
HpaII CCGG 2 cut(s) 180, 197
HphI GGTGA 3 cut(s) 98, 104, 169
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 2 cut(s) 220, 365
Hpy188III TCNNGA 3 cut(s) 15, 197, 334
Hpy8I GTNNAC 1 cut(s) 228
Hpy99I CGWCG 2 cut(s) 109, 136
HpyAV CCTTC 1 cut(s) 512
HpyCH4III ACNGT 3 cut(s) 80, 86, 666
HpyCH4IV ACGT 1 cut(s) 473
HpyCH4V TGCA 6 cut(s) 4, 39, 206, 435, 464, 553
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 485
HpyF3I CTNAG 1 cut(s) 603
HpySE526I ACGT 1 cut(s) 473
Hsp92II CATG 2 cut(s) 153, 642
HspAI GCGC 1 cut(s) 629
Kpn2I TCCGGA 1 cut(s) 196
Kzo9I GATC 2 cut(s) 120, 162
LguI GCTCTTC 1 cut(s) 16
LmnI GCTCC 3 cut(s) 106, 221, 650
LpnPI CCDG 7 cut(s) 125, 152, 193, 210, 226, 326, 366
Lsp1109I GCAGC 2 cut(s) 219, 428
LweI GCATC 3 cut(s) 13, 193, 316
MaeI CTAG 2 cut(s) 264, 480
MaeII ACGT 1 cut(s) 473
MaeIII GTNAC 2 cut(s) 80, 86
MalI GATC 2 cut(s) 122, 164
MboI GATC 2 cut(s) 120, 162
MboII GAAGA 7 cut(s) 3, 61, 166, 268, 279, 508, 664
MluCI AATT 5 cut(s) 270, 467, 505, 557, 595
MnlI CCTC 6 cut(s) 107, 136, 193, 202, 359, 541
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 196
MseI TTAA 2 cut(s) 509, 543
MspI CCGG 2 cut(s) 180, 197
MspR9I CCNGG 1 cut(s) 140
Mva1269I GAATGC 3 cut(s) 265, 460, 631
MvaI CCWGG 1 cut(s) 140
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 2 cut(s) 320, 485
NdeII GATC 2 cut(s) 120, 162
NlaIII CATG 2 cut(s) 153, 642
NlaIV GGNNCC 1 cut(s) 646
NmuCI GTSAC 1 cut(s) 86
NruI TCGCGA 1 cut(s) 16
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 191
PciSI GCTCTTC 1 cut(s) 16
PcsI WCGNNNNNNNCGW 2 cut(s) 13, 56
PctI GAATGC 3 cut(s) 265, 460, 631
PfeI GAWTC 4 cut(s) 18, 221, 374, 388
PkrI GCNGC 3 cut(s) 234, 418, 674
PmaCI CACGTG 1 cut(s) 474
PmlI CACGTG 1 cut(s) 474
Ppu21I YACGTR 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 138
PspCI CACGTG 1 cut(s) 474
PspEI GGTNACC 1 cut(s) 86
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 646
PspPI GGNCC 1 cut(s) 580
RruI TCGCGA 1 cut(s) 16
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 2 cut(s) 509, 543
SatI GCNGC 3 cut(s) 233, 417, 673
Sau3AI GATC 2 cut(s) 120, 162
Sau96I GGNCC 1 cut(s) 580
ScrFI CCNGG 1 cut(s) 140
SetI ASST 7 cut(s) 99, 141, 218, 316, 476, 552, 585
SfaNI GCATC 3 cut(s) 13, 193, 316
SinI GGWCC 1 cut(s) 580
Sse9I AATT 5 cut(s) 270, 467, 505, 557, 595
SseBI AGGCCT 1 cut(s) 191
SsiI CCGC 4 cut(s) 91, 115, 245, 672
SspMI CTAG 2 cut(s) 264, 480
StuI AGGCCT 1 cut(s) 191
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 1 cut(s) 533
TaaI ACNGT 3 cut(s) 80, 86, 666
TaiI ACGT 1 cut(s) 476
TaqI TCGA 3 cut(s) 50, 238, 333
TasI AATT 5 cut(s) 270, 467, 505, 557, 595
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 4 cut(s) 18, 221, 374, 388
Tru1I TTAA 2 cut(s) 509, 543
Tru9I TTAA 2 cut(s) 509, 543
TscAI CASTG 2 cut(s) 313, 385
TseFI GTSAC 1 cut(s) 86
TseI GCWGC 2 cut(s) 232, 416
Tsp45I GTSAC 1 cut(s) 86
TspDTI ATGAA 2 cut(s) 170, 269
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 2 cut(s) 313, 385
VpaK11BI GGWCC 1 cut(s) 580
XapI RAATTY 2 cut(s) 467, 505
XcmI CCANNNNNNNNNTGG 2 cut(s) 136, 595
XspI CTAG 2 cut(s) 264, 480
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.