Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0015641

Physical Location & Seq

Genomic Coordinates Reverse (-)

32329077 .. 32331387

Gene Structure 2311 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1255100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGTCCAAAGCCTTAGCCATTCACTCAGTCAAGACTCAGGGCAGTGCCAGTGTCTTTCTTCCAGAACAAGAATGGAAAAACTGCAGCGGCCCTTTTCAGAAGGAGCGAAACGTGTCTGCAAAAAACTGTCATTTTGATGGCCTTTACTACGGTAGCAGTGCGTGCTCGAACACAACTCTTGCATCCATCAAGAAAGAGCAGGCCTTCCAAAACGCGATAAGTAATTGCTCAAACCTTAGCAATGCATTTGAGGATGTTTGTGGAAATTGCAGCAAGGCCGTATTAGATTTGAGGGACGATTTACTGGAAGCATATGATGTGAAGGATGGGAACGCAACTGAGACGGGCATATGTGCTATGGCAGCTGTTATTTCTGTTTTAGAAGAGAAGATGAACAATACCTTTTCGATCGACAATGACTATATGTTTTGTATGTCTTTGTTAGACACCTTCGGCATCACCGCAGTGACGCTGATCATCCTGCTGATAAAATTTGTGACCAAGAAGCAGCCTCAAAAACCGGTTCACTCAGGACCGATTACTACATGGTCTGGCTTGTACAGGTTCTCCAAGGCCGAGATTGAGAATGCCATTAATACTGAAAGAAAGGACCTTGGACGAGGAAGCGCTGGTCAAGTTTATAAAGGGGTTCTTCCGAGCGGGCAAGTTGTGGCCATTAAGCACATAAACAAGAGCAACACATCCGATTCATTTACTAGAGAAGTGGAAGGCCTTTCGAGGATTCGACATCCAAACTTGGTTTGCCTCTTTGGTTGCTGCGTCGAAGATGGCGAACAATATCTTGTGTATGAATATTGCGCTAAAGGGAATCTAGCTCAACACCTCCTAAGTGACGATCCTGTCTTAACATGGGAAAGAAGAGTTAAGATTCTAAGAGATTGTGCACTTGCTCTGAGATATCTCCACCATTATATTGATGGATGCATTGTCCATAGAGATATTAAGCTTACAAACATCCTTTTGACGGATAACTTGGAACCCAAGCTATCTGATTTCGGGCTGGCAAAGATGTTGGGTATGGAGGAGACCAAAGTATTTACAGATGTTAGAGGAACCATAGGCTACATGGATCCAGAGTACATGACCAATGCCAAGTTAACCTGCGCGAGTGATGTCTACAGTTTCGGTATTGTTGCTCTGCAACTGCTATCGGGGCAAAAAGTATTTGAGCTGGATCTTGATGCTAGAGACCAACTAACAAGAAAGGCAAAGGATATAAGTATGAACAAACGCCCTCCTGAGGATCTTGAGGACCCAAGACTGAATGGAAATGTGAACAAGGCGGACTTTGAATCGATCCTTCAAGTTGCAGTACTTTGCGTTGCCAAATCAAGCAAAGGCCGCCCTACCATCGGTCTTGTTTTCGAAGAGATGGACAAGGCTTGGAAGAACACTCTTGCTGACATGAAGGCAAGGAATAAGGGGATAAGTTCATCAGCAACACCACTGTCTATGTCTTCCGATGTGTTTTCAGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase

Protein Analysis

500

Amino Acids

55.21

Weight (kDa)

6.19

Isoelectric Point (pI)

36.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	202 - 458	1.6e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	202 - 396	1.7e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015641)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g01140
malus_domestica	MD04G1236100.v1.1 MD12G1255100.v1.1
prunus_persica	Prupe.6G356000_v2.0.a1
pyrus_communis	pycom04g20980 pycom12g23380
rosa_chinensis	RchiOBHm_Chr3g0448671
rosa_laevigata	RLG00000025880
rosa_multiflora	Rmu_sc0003116.1_g000033
rosa_roxburghii	Rroxscaffold_6G00426940
rosa_rugosa	Rorug02G0615200
rosa_samantha	Rh3AG014000 Rh3BG013800 Rh3CG012900 Rh3DG014600
rosa_wichuraiana	Rw3G001040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	642
AasI	GACNNNNNNGTC	1 cut(s)	860
Acc36I	ACCTGC	1 cut(s)	1130
AccBSI	CCGCTC	1 cut(s)	660
AccI	GTMKAC	1 cut(s)	1137
AccII	CGCG	2 cut(s)	215, 1127
AciI	CCGC	5 cut(s)	87, 462, 660, 1304, 1363
AclWI	GGATC	6 cut(s)	851, 1085, 1098, 1203, 1272, 1312
AcoI	YGGCCR	1 cut(s)	672
AcsI	RAATTY	1 cut(s)	491
AfaI	GTAC	3 cut(s)	560, 1100, 1335
AfeI	AGCGCT	1 cut(s)	628
AfiI	CCNNNNNNNGG	3 cut(s)	985, 1261, 1373
AflIII	ACRYGT	1 cut(s)	111
AgeI	ACCGGT	1 cut(s)	520
AgsI	TTSAA	2 cut(s)	1313, 1325
AleI	CACNNNNGTG	1 cut(s)	464
AluBI	AGCT	5 cut(s)	365, 836, 967, 1006, 1192
AluI	AGCT	5 cut(s)	365, 836, 967, 1006, 1192
Alw21I	GWGCWC	2 cut(s)	167, 907
Alw26I	GTCTC	3 cut(s)	335, 1040, 1203
Alw44I	GTGCAC	1 cut(s)	903
AlwI	GGATC	6 cut(s)	851, 1085, 1098, 1203, 1272, 1312
Aor51HI	AGCGCT	1 cut(s)	628
AoxI	GGCC	8 cut(s)	88, 139, 201, 276, 573, 672, 730, 1360
ApaLI	GTGCAC	1 cut(s)	903
ApeKI	GCWGC	5 cut(s)	84, 270, 362, 508, 777
ApoI	RAATTY	1 cut(s)	491
AseI	ATTAAT	1 cut(s)	594
AsiGI	ACCGGT	1 cut(s)	520
AspLEI	GCGC	3 cut(s)	629, 821, 1127
AspS9I	GGNCC	4 cut(s)	89, 533, 610, 1273
AsuHPI	GGTGA	1 cut(s)	451
AsuII	TTCGAA	1 cut(s)	1386
AvaII	GGWCC	3 cut(s)	533, 610, 1273
AxyI	CCTNAGG	1 cut(s)	1260
BaeGI	GKGCMC	1 cut(s)	907
BalI	TGGCCA	1 cut(s)	674
BamHI	GGATCC	1 cut(s)	1090
BbsI	GAAGAC	1 cut(s)	1470
Bbv12I	GWGCWC	2 cut(s)	167, 907
BbvI	GCAGC	5 cut(s)	96, 282, 374, 520, 764
BccI	CCATC	7 cut(s)	131, 194, 320, 782, 932, 1379, 1387
BceAI	ACGGC	1 cut(s)	263
BclI	TGATCA	1 cut(s)	474
BcoDI	GTCTC	3 cut(s)	335, 1040, 1203
BfaI	CTAG	3 cut(s)	717, 833, 1206
BfmI	CTRYAG	2 cut(s)	82, 1138
BfoI	RGCGCY	1 cut(s)	630
BfuAI	ACCTGC	1 cut(s)	1130
BisI	GCNGC	7 cut(s)	85, 88, 271, 363, 509, 778, 1363
BlsI	GCNGC	7 cut(s)	86, 89, 272, 364, 510, 779, 1364
BmcAI	AGTACT	1 cut(s)	1335
Bme18I	GGWCC	3 cut(s)	533, 610, 1273
BmgT120I	GGNCC	4 cut(s)	89, 533, 610, 1273
BmiI	GGNNCC	4 cut(s)	999, 1075, 1092, 1275
BmsI	GCATC	4 cut(s)	191, 465, 932, 1192
BpiI	GAAGAC	1 cut(s)	1470
Bpu10I	CCTNAGC	2 cut(s)	13, 236
Bpu14I	TTCGAA	1 cut(s)	1386
BpuEI	CTTGAG	1 cut(s)	1289
Bsa29I	ATCGAT	1 cut(s)	1316
BsaI	GGTCTC	2 cut(s)	1040, 1203
BsaJI	CCNNGG	2 cut(s)	570, 613
BsaWI	WCCGGW	1 cut(s)	520
BsaXI	ACNNNNNCTCC	2 cut(s)	551, 581
Bsc4I	CCNNNNNNNGG	3 cut(s)	985, 1261, 1373
Bse118I	RCCGGY	1 cut(s)	520
Bse1I	ACTGG	2 cut(s)	48, 309
Bse21I	CCTNAGG	1 cut(s)	1260
Bse3DI	GCAATG	1 cut(s)	247
BseCI	ATCGAT	1 cut(s)	1316
BseDI	CCNNGG	2 cut(s)	570, 613
BseGI	GGATG	8 cut(s)	182, 259, 331, 477, 701, 748, 947, 975
BseLI	CCNNNNNNNGG	3 cut(s)	985, 1261, 1373
BseMI	GCAATG	1 cut(s)	247
BseMII	CTCAG	6 cut(s)	39, 50, 330, 543, 905, 1251
BseNI	ACTGG	2 cut(s)	48, 309
BseRI	GAGGAG	1 cut(s)	1058
BseSI	GKGCMC	1 cut(s)	907
BseXI	GCAGC	5 cut(s)	96, 282, 374, 520, 764
Bsh1236I	CGCG	2 cut(s)	215, 1127
Bsh1285I	CGRYCG	1 cut(s)	411
BshFI	GGCC	8 cut(s)	90, 141, 203, 278, 575, 674, 732, 1362
BshTI	ACCGGT	1 cut(s)	520
BshVI	ATCGAT	1 cut(s)	1316
BsiEI	CGRYCG	1 cut(s)	411
BsiHKAI	GWGCWC	2 cut(s)	167, 907
BsiSI	CCGG	1 cut(s)	521
BslFI	GGGAC	1 cut(s)	308
BslI	CCNNNNNNNGG	3 cut(s)	985, 1261, 1373
BsmAI	GTCTC	3 cut(s)	335, 1040, 1203
BsmBI	CGTCTC	1 cut(s)	335
BsmFI	GGGAC	1 cut(s)	308
BsmI	GAATGC	1 cut(s)	592
BsnI	GGCC	8 cut(s)	90, 141, 203, 278, 575, 674, 732, 1362
Bso31I	GGTCTC	2 cut(s)	1040, 1203
Bsp119I	TTCGAA	1 cut(s)	1386
Bsp1286I	GDGCHC	2 cut(s)	167, 907
Bsp1407I	TGTACA	1 cut(s)	558
Bsp143I	GATC	7 cut(s)	408, 474, 856, 1090, 1195, 1264, 1317
BspACI	CCGC	5 cut(s)	87, 462, 660, 1304, 1363
BspANI	GGCC	8 cut(s)	90, 141, 203, 278, 575, 674, 732, 1362
BspCNI	CTCAG	6 cut(s)	38, 49, 331, 542, 906, 1252
BspDI	ATCGAT	1 cut(s)	1316
BspFNI	CGCG	2 cut(s)	215, 1127
BspLI	GGNNCC	4 cut(s)	999, 1075, 1092, 1275
BspMAI	CTGCAG	1 cut(s)	86
BspMI	ACCTGC	1 cut(s)	1130
BspPI	GGATC	6 cut(s)	851, 1085, 1098, 1203, 1272, 1312
BspT104I	TTCGAA	1 cut(s)	1386
BspTNI	GGTCTC	2 cut(s)	1040, 1203
BsrBI	CCGCTC	1 cut(s)	660
BsrDI	GCAATG	1 cut(s)	247
BsrFI	RCCGGY	1 cut(s)	520
BsrGI	TGTACA	1 cut(s)	558
BsrI	ACTGG	2 cut(s)	48, 309
BssAI	RCCGGY	1 cut(s)	520
BssECI	CCNNGG	2 cut(s)	570, 613
BssMI	GATC	7 cut(s)	408, 474, 856, 1090, 1195, 1264, 1317
BssT1I	CCWWGG	2 cut(s)	570, 613
Bst4CI	ACNGT	4 cut(s)	128, 152, 1142, 1470
Bst6I	CTCTTC	3 cut(s)	378, 874, 1383
BstAPI	GCANNNNNTGC	1 cut(s)	162
BstAUI	TGTACA	1 cut(s)	558
BstBI	TTCGAA	1 cut(s)	1386
BstC8I	GCNNGC	4 cut(s)	163, 201, 662, 1023
BstF5I	GGATG	8 cut(s)	182, 259, 331, 477, 701, 748, 947, 975
BstFNI	CGCG	2 cut(s)	215, 1127
BstH2I	RGCGCY	1 cut(s)	630
BstHHI	GCGC	3 cut(s)	629, 821, 1127
BstKTI	GATC	7 cut(s)	411, 477, 859, 1093, 1198, 1267, 1320
BstMAI	GTCTC	3 cut(s)	335, 1040, 1203
BstMBI	GATC	7 cut(s)	408, 474, 856, 1090, 1195, 1264, 1317
BstMCI	CGRYCG	1 cut(s)	411
BstMWI	GCNNNNNNNGC	4 cut(s)	162, 362, 1174, 1362
BstSFI	CTRYAG	2 cut(s)	82, 1138
BstSLI	GKGCMC	1 cut(s)	907
BstUI	CGCG	2 cut(s)	215, 1127
BstV1I	GCAGC	5 cut(s)	96, 282, 374, 520, 764
BstV2I	GAAGAC	1 cut(s)	1470
BstX2I	RGATCY	3 cut(s)	1090, 1195, 1264
BstYI	RGATCY	3 cut(s)	1090, 1195, 1264
Bsu15I	ATCGAT	1 cut(s)	1316
Bsu36I	CCTNAGG	1 cut(s)	1260
BsuRI	GGCC	8 cut(s)	90, 141, 203, 278, 575, 674, 732, 1362
BsuTUI	ATCGAT	1 cut(s)	1316
BtsCI	GGATG	8 cut(s)	182, 259, 331, 477, 701, 748, 947, 975
BtsI	GCAGTG	3 cut(s)	49, 163, 471
BtsIMutI	CAGTG	5 cut(s)	49, 55, 163, 471, 1466
BveI	ACCTGC	1 cut(s)	1130
Cac8I	GCNNGC	4 cut(s)	163, 201, 662, 1023
CfoI	GCGC	3 cut(s)	629, 821, 1127
Cfr10I	RCCGGY	1 cut(s)	520
Cfr13I	GGNCC	4 cut(s)	89, 533, 610, 1273
ClaI	ATCGAT	1 cut(s)	1316
CseI	GACGC	2 cut(s)	478, 769
Csp6I	GTAC	3 cut(s)	559, 1099, 1334
CspAI	ACCGGT	1 cut(s)	520
CviAII	CATG	5 cut(s)	546, 870, 1087, 1102, 1426
CviQI	GTAC	3 cut(s)	559, 1099, 1334
DpnI	GATC	7 cut(s)	410, 476, 858, 1092, 1197, 1266, 1319
DpnII	GATC	7 cut(s)	408, 474, 856, 1090, 1195, 1264, 1317
DrdI	GACNNNNNNGTC	1 cut(s)	860
DseDI	GACNNNNNNGTC	1 cut(s)	860
EaeI	YGGCCR	1 cut(s)	672
Eam1104I	CTCTTC	3 cut(s)	378, 874, 1383
EarI	CTCTTC	3 cut(s)	378, 874, 1383
EciI	GGCGGA	1 cut(s)	1319
Eco130I	CCWWGG	2 cut(s)	570, 613
Eco147I	AGGCCT	2 cut(s)	203, 732
Eco31I	GGTCTC	2 cut(s)	1040, 1203
Eco32I	GATATC	1 cut(s)	920
Eco47I	GGWCC	3 cut(s)	533, 610, 1273
Eco47III	AGCGCT	1 cut(s)	628
Eco81I	CCTNAGG	1 cut(s)	1260
EcoO109I	RGGNCCY	2 cut(s)	610, 1273
EcoRV	GATATC	1 cut(s)	920
EcoT14I	CCWWGG	2 cut(s)	570, 613
EcoT22I	ATGCAT	2 cut(s)	247, 947
ErhI	CCWWGG	2 cut(s)	570, 613
Esp3I	CGTCTC	1 cut(s)	335
FaeI	CATG	5 cut(s)	549, 873, 1090, 1105, 1429
FalI	AAGNNNNNCTT	4 cut(s)	636, 668, 1292, 1324
FaqI	GGGAC	1 cut(s)	308
FatI	CATG	5 cut(s)	545, 869, 1086, 1101, 1425
FauI	CCCGC	1 cut(s)	653
FauNDI	CATATG	2 cut(s)	313, 350
FbaI	TGATCA	1 cut(s)	474
FblI	GTMKAC	1 cut(s)	1137
Fnu4HI	GCNGC	7 cut(s)	85, 88, 271, 363, 509, 778, 1363
FokI	GGATG	8 cut(s)	169, 266, 338, 464, 688, 735, 954, 962
Fsp4HI	GCNGC	7 cut(s)	85, 88, 271, 363, 509, 778, 1363
FspBI	CTAG	3 cut(s)	717, 833, 1206
GlaI	GCGC	3 cut(s)	628, 820, 1126
GluI	GCNGC	7 cut(s)	85, 88, 271, 363, 509, 778, 1363
HaeII	RGCGCY	1 cut(s)	630
HaeIII	GGCC	8 cut(s)	90, 141, 203, 278, 575, 674, 732, 1362
HapII	CCGG	1 cut(s)	521
HgaI	GACGC	2 cut(s)	478, 769
HhaI	GCGC	3 cut(s)	629, 821, 1127
Hin1II	CATG	5 cut(s)	549, 873, 1090, 1105, 1429
Hin6I	GCGC	3 cut(s)	627, 819, 1125
HinP1I	GCGC	3 cut(s)	627, 819, 1125
HincII	GTYRAC	1 cut(s)	1119
HindII	GTYRAC	1 cut(s)	1119
HindIII	AAGCTT	1 cut(s)	965
HinfI	GANTC	6 cut(s)	34, 707, 742, 829, 889, 1313
HpaI	GTTAAC	1 cut(s)	1119
HpaII	CCGG	1 cut(s)	521
HphI	GGTGA	1 cut(s)	451
Hpy166II	GTNNAC	5 cut(s)	526, 905, 1119, 1138, 1297
Hpy188I	TCNGA	7 cut(s)	99, 657, 706, 915, 1012, 1483, 1499
Hpy188III	TCNNGA	8 cut(s)	31, 62, 190, 531, 1094, 1199, 1259, 1268
Hpy8I	GTNNAC	5 cut(s)	526, 905, 1119, 1138, 1297
Hpy99I	CGWCG	1 cut(s)	785
HpyAV	CCTTC	7 cut(s)	94, 214, 316, 460, 722, 1331, 1423
HpyCH4III	ACNGT	4 cut(s)	128, 152, 1142, 1470
HpyCH4IV	ACGT	1 cut(s)	111
HpyCH4V	TGCA	9 cut(s)	84, 119, 182, 245, 270, 905, 945, 1162, 1331
HpyF10VI	GCNNNNNNNGC	4 cut(s)	162, 362, 1174, 1362
HpySE526I	ACGT	1 cut(s)	111
Hsp92II	CATG	5 cut(s)	549, 873, 1090, 1105, 1429
HspAI	GCGC	3 cut(s)	627, 819, 1125
Ksp22I	TGATCA	1 cut(s)	474
KspAI	GTTAAC	1 cut(s)	1119
Kzo9I	GATC	7 cut(s)	408, 474, 856, 1090, 1195, 1264, 1317
LmnI	GCTCC	1 cut(s)	103
Lsp1109I	GCAGC	5 cut(s)	96, 282, 374, 520, 764
LweI	GCATC	4 cut(s)	191, 465, 932, 1192
MaeI	CTAG	3 cut(s)	717, 833, 1206
MaeII	ACGT	1 cut(s)	111
MaeIII	GTNAC	3 cut(s)	466, 496, 851
MalI	GATC	7 cut(s)	410, 476, 858, 1092, 1197, 1266, 1319
MbiI	CCGCTC	1 cut(s)	660
MboI	GATC	7 cut(s)	408, 474, 856, 1090, 1195, 1264, 1317
MboII	GAAGA	9 cut(s)	50, 395, 400, 644, 797, 891, 1400, 1420, 1470
MflI	RGATCY	3 cut(s)	1090, 1195, 1264
MhlI	GDGCHC	2 cut(s)	167, 907
MlsI	TGGCCA	1 cut(s)	674
MluCI	AATT	3 cut(s)	223, 265, 491
MluNI	TGGCCA	1 cut(s)	674
MlyI	GAGTC	1 cut(s)	28
Mox20I	TGGCCA	1 cut(s)	674
Mph1103I	ATGCAT	2 cut(s)	247, 947
MscI	TGGCCA	1 cut(s)	674
MseI	TTAA	6 cut(s)	594, 678, 866, 885, 963, 1118
MslI	CAYNNNNRTG	2 cut(s)	135, 464
Msp20I	TGGCCA	1 cut(s)	674
MspA1I	CMGCKG	2 cut(s)	87, 365
MspI	CCGG	1 cut(s)	521
Mva1269I	GAATGC	1 cut(s)	592
MvnI	CGCG	2 cut(s)	215, 1127
MwoI	GCNNNNNNNGC	4 cut(s)	162, 362, 1174, 1362
NdeI	CATATG	2 cut(s)	313, 350
NdeII	GATC	7 cut(s)	408, 474, 856, 1090, 1195, 1264, 1317
NlaIII	CATG	5 cut(s)	549, 873, 1090, 1105, 1429
NlaIV	GGNNCC	4 cut(s)	999, 1075, 1092, 1275
NmeAIII	GCCGAG	1 cut(s)	601
NmuCI	GTSAC	3 cut(s)	466, 496, 851
NsiI	ATGCAT	2 cut(s)	247, 947
NspV	TTCGAA	1 cut(s)	1386
OliI	CACNNNNGTG	1 cut(s)	464
PceI	AGGCCT	2 cut(s)	203, 732
PcsI	WCGNNNNNNNCGW	1 cut(s)	789
PctI	GAATGC	1 cut(s)	592
PfeI	GAWTC	5 cut(s)	707, 742, 829, 889, 1313
PinAI	ACCGGT	1 cut(s)	520
PkrI	GCNGC	7 cut(s)	86, 89, 272, 364, 510, 779, 1364
Ple19I	CGATCG	1 cut(s)	411
PleI	GAGTC	1 cut(s)	28
PpsI	GAGTC	1 cut(s)	28
PpuMI	RGGWCCY	2 cut(s)	610, 1273
PshBI	ATTAAT	1 cut(s)	594
PsiI	TTATAA	1 cut(s)	642
Psp5II	RGGWCCY	2 cut(s)	610, 1273
PspN4I	GGNNCC	4 cut(s)	999, 1075, 1092, 1275
PspPI	GGNCC	4 cut(s)	89, 533, 610, 1273
PspPPI	RGGWCCY	2 cut(s)	610, 1273
PstI	CTGCAG	1 cut(s)	86
PsuI	RGATCY	3 cut(s)	1090, 1195, 1264
PvuI	CGATCG	1 cut(s)	411
PvuII	CAGCTG	1 cut(s)	365
RsaI	GTAC	3 cut(s)	560, 1100, 1335
RsaNI	GTAC	3 cut(s)	559, 1099, 1334
RseI	CAYNNNNRTG	2 cut(s)	135, 464
SaqAI	TTAA	6 cut(s)	594, 678, 866, 885, 963, 1118
SatI	GCNGC	7 cut(s)	85, 88, 271, 363, 509, 778, 1363
Sau3AI	GATC	7 cut(s)	408, 474, 856, 1090, 1195, 1264, 1317
Sau96I	GGNCC	4 cut(s)	89, 533, 610, 1273
ScaI	AGTACT	1 cut(s)	1335
SchI	GAGTC	1 cut(s)	28
SduI	GDGCHC	2 cut(s)	167, 907
SfaNI	GCATC	4 cut(s)	191, 465, 932, 1192
SfcI	CTRYAG	2 cut(s)	82, 1138
SfuI	TTCGAA	1 cut(s)	1386
SinI	GGWCC	3 cut(s)	533, 610, 1273
SmiMI	CAYNNNNRTG	2 cut(s)	135, 464
SmlI	CTYRAG	1 cut(s)	1268
SmoI	CTYRAG	1 cut(s)	1268
Sse9I	AATT	3 cut(s)	223, 265, 491
SseBI	AGGCCT	2 cut(s)	203, 732
SsiI	CCGC	5 cut(s)	87, 462, 660, 1304, 1363
SspI	AATATT	1 cut(s)	815
SspMI	CTAG	3 cut(s)	717, 833, 1206
StuI	AGGCCT	2 cut(s)	203, 732
StyI	CCWWGG	2 cut(s)	570, 613
TaaI	ACNGT	4 cut(s)	128, 152, 1142, 1470
TaiI	ACGT	1 cut(s)	114
TaqI	TCGA	8 cut(s)	167, 407, 411, 737, 745, 783, 1316, 1386
TaqII	GACCGA	2 cut(s)	550, 1364
TasI	AATT	3 cut(s)	223, 265, 491
TatI	WGTACW	3 cut(s)	558, 1098, 1333
TauI	GCSGC	2 cut(s)	90, 1365
TfiI	GAWTC	5 cut(s)	707, 742, 829, 889, 1313
Tru1I	TTAA	6 cut(s)	594, 678, 866, 885, 963, 1118
Tru9I	TTAA	6 cut(s)	594, 678, 866, 885, 963, 1118
TscAI	CASTG	5 cut(s)	49, 55, 163, 471, 1473
TseFI	GTSAC	3 cut(s)	466, 496, 851
TseI	GCWGC	5 cut(s)	84, 270, 362, 508, 777
Tsp45I	GTSAC	3 cut(s)	466, 496, 851
TspDTI	ATGAA	6 cut(s)	407, 699, 825, 1259, 1442, 1443
TspGWI	ACGGA	1 cut(s)	1001
TspRI	CASTG	5 cut(s)	49, 55, 163, 471, 1473
VneI	GTGCAC	1 cut(s)	903
VpaK11BI	GGWCC	3 cut(s)	533, 610, 1273
VspI	ATTAAT	1 cut(s)	594
XapI	RAATTY	1 cut(s)	491
XcmI	CCANNNNNNNNNTGG	3 cut(s)	69, 935, 1084
XmiI	GTMKAC	1 cut(s)	1137
XspI	CTAG	3 cut(s)	717, 833, 1206
ZrmI	AGTACT	1 cut(s)	1335
Zsp2I	ATGCAT	2 cut(s)	247, 947

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.