Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0015398

Physical Location & Seq

Genomic Coordinates Reverse (-)

1246553 .. 1250362

Gene Structure 3810 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1019800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2013 bp

ATGGCTGCTTTGCAGAGAGTGGCGCAGTCAAGCGTGAGCACTACTGCGAGTAGTTACGGTTCGTGTAAAGTTGGTGGTGGTGTTTTGTCACCGTCTGCCGGCATTGAAATGGCAGTCCCCAATCAGTTTCCGGCGGGCTTGAGGGTTCTAGTGGTGGACGATGACACCACGTGCTTGAGGATTCTTGAGCTGATGTTACTCCGGTGTCTGTACCAGGTTACCACTTGTTCCGAGGCTACTGTTGCTTTGAATCTTCTACGAGAGAGGAAAGACTGTTTTGATGTCGTACTGAGTGATGTCCATATGCCTGATATGGATGGATTTAAACTCCTGGAACACGTTGGGCTGGAAATGGACCTTCCTGTTATCATGATGTCTGCTGATGGGAGAACAAGTGTTGTTATGAGAGGAATTAGACATGGAGCCTGTGATTTTTTAATTAAGCCTATAAGTGAGGCAGAACTCAAGAATATTTGGCAGCATGTTGTTAGGAAAAAATGGAATGGAAGTAAAGAACTTGAACATTCTGGGAGCTTGGAAGATAATGATCCACATAAACGGGGAAACAATGATTTTGAATACGCATCTTCTGTTAACGAAGGAACAGAAGTTTCATTGAAAGGTCATAAGAAGAGGATCAACCCTAAAGAAGATGATGATGGTGATACAGAAAACGATGATTTGTCTACATCGAAGAAACCTCGTGTAGTATGGTCAGTTGAACTCCACCAGCAATTTGTCACTGCTGTGAACCAGCTTGGCCTTGATAAGGCTGTACCAAAGAGAATTCTTGAATTGATGAATGTACCTGGTTTAACTCGAGAAAATGTTGCTAGCCATTTGCAGAAATTCAGGTTATATTTGAAGAGATTAAGTGGAGTAGCTCAACAACAAAGTGGGATTGCAAATCCTTTATGTGGGCCCGTGGACTCAAATGGGAAGTTGGGTTCGCTTAGTAGATTTGACTTCCAAGCTTTGGCTGCCTCTGGACAAATTCCTCCGCAAACACTAGCAGCCCTGCAAGCTGAGCTTTTAGGTCAACCTGCAGGGAACCTTGTGCCAGCAATGGATCAGCCTGCACTTCTACATGCATCCCTACAAGCACCCAAGCGTCCCCCTGTTGAACATGGTGTGCCATTCATGCAGCCTTTCGTAAAAAGCCAATCAAACGTTTCCAAACATTTTCCACAATCCGTTATTTCTGCTGAGGATGCATCTCTGGGATTTGGACAATGGAGGTCTAATAGTCGTAGTACAGTGGCACCCAGCAATGATCATGGAGGGTTGAGTACTCAGAACAGTAACTTGTTGATGGGAATTGTGCCGCAGGAGCAAAGACAACATAAAAGAACGCAGCAGCAGTCTGTGCTAACCGAACCTAGCCGTTCATTTAATGTGCAGCCATCTTGCCTTGTGGTCCCCTCTCAATCATCAACTGGTTTCCAGGCTGGAAATAGTCCTGCTTCTGTTAATCAGAGTAGTAGCTTTAACAGGTCTACTGTTGTTGATTACAGTCTGCCTTCAGATCAATCTAATAATTCCTTGAATGTTGGACATATACCGACTGGAAATCCTAAAACTAGTGGCATTCTTGGTGGGTATTCTGGCCCTGGTTCTATTTGTGCCACGTCTTGCTTAGTTAATGCTGACAATAGCACCAGTTACCAGAACTCAACTGCGACATTTAGTGATTCCAGAGAGTTGCCGGGGTTTTTGCATAATACGGCAAACAGCCATGGTTTTTATGTTGATAAGTCAGGGGAAATGCTTGATCAGGGACCTCTTAGGAATCTCGGATTTGTTGGTAAAGAGACTTGCATTCCAAGTCGATTTGCAGTGGATGATTTTGAATCGCAAATGAGCAACTTGAACCCTGGAAGGATCCATGTGGAGAGCAGTGGTACCCTAGTCAAGCAGGAGCCAAGTGAGGATTATGTGGATAACGCAAAACTAGGTATCCCAATATTACACCAATATTCTTCAAGTGATTTCATGAGTCCTTTCGCTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000156 GO:0000160 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009873 GO:0009888 GO:0009889 GO:0009987 GO:0010015 GO:0010033 GO:0010073 GO:0010075 GO:0010082 GO:0010119 GO:0010150 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022622 GO:0023052 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032870 GO:0035556 GO:0040008 GO:0042221 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071369 GO:0071495 GO:0080050 GO:0080090 GO:0080113 GO:0090693 GO:0099402 GO:0140110 GO:1903506 GO:1905392 GO:2000026 GO:2000112 GO:2000241 GO:2000280 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Myb_DNA-binding Response_reg

Protein Analysis

671

Amino Acids

72.52

Weight (kDa)

5.96

Isoelectric Point (pI)

53.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Response_reg	PF00072	49 - 157	7.6e-22	Response regulator receiver domain
Myb_DNA-binding	PF00249	235 - 284	1.7e-07	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0015398)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01760 AT2G01760 AT2G01760
fragaria_vesca	FvH4_4g33470
malus_domestica	MD13G1019800.v1.1 MD16G1017900.v1.1
prunus_persica	Prupe.1G334200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0442781
rosa_multiflora	Rmu_ssc0000204.1_g000021
rosa_roxburghii	Rroxscaffold_5G00383560
rosa_rugosa	Rorug04G0343000
rosa_samantha	Rh4AG395600 Rh4BG408300 Rh4CG424100 Rh4DG402500
rosa_wichuraiana	Rw4G034190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1051
Acc65I	GGTACC	1 cut(s)	1901
AccB1I	GGYRCC	2 cut(s)	1261, 1901
AccB7I	CCANNNNNTGG	1 cut(s)	976
AccI	GTMKAC	2 cut(s)	686, 1496
AciI	CCGC	3 cut(s)	134, 1001, 1325
AclI	AACGTT	1 cut(s)	1170
AclWI	GGATC	5 cut(s)	542, 644, 1077, 1876, 1889
AcsI	RAATTY	3 cut(s)	786, 848, 993
AcuI	CTGAAG	1 cut(s)	1506
AcvI	CACGTG	1 cut(s)	171
AdeI	CACNNNGTG	1 cut(s)	171
AfaI	GTAC	7 cut(s)	212, 288, 777, 807, 1255, 1291, 1903
AfiI	CCNNNNNNNGG	4 cut(s)	98, 769, 917, 976
AflIII	ACRYGT	1 cut(s)	337
AhlI	ACTAGT	1 cut(s)	1580
AjiI	CACGTC	1 cut(s)	1629
AjnI	CCWGG	6 cut(s)	213, 330, 808, 1443, 1609, 1873
AleI	CACNNNNGTG	1 cut(s)	746
AloI	GAACNNNNNNTCC	2 cut(s)	931, 963
AluBI	AGCT	8 cut(s)	190, 534, 757, 884, 974, 1025, 1030, 1485
AluI	AGCT	8 cut(s)	190, 534, 757, 884, 974, 1025, 1030, 1485
Alw21I	GWGCWC	1 cut(s)	41
Alw26I	GTCTC	1 cut(s)	1805
AlwI	GGATC	5 cut(s)	542, 644, 1077, 1876, 1889
Ama87I	CYCGRG	1 cut(s)	819
AoxI	GGCC	3 cut(s)	760, 920, 1606
ApaI	GGGCCC	1 cut(s)	924
ApeKI	GCWGC	8 cut(s)	5, 478, 980, 1013, 1144, 1354, 1357, 1399
ApoI	RAATTY	3 cut(s)	786, 848, 993
Asp718I	GGTACC	1 cut(s)	1901
AspLEI	GCGC	1 cut(s)	25
AspS9I	GGNCC	6 cut(s)	355, 920, 921, 1417, 1607, 1778
AsuC2I	CCSGG	1 cut(s)	1707
AsuHPI	GGTGA	2 cut(s)	81, 674
AsuNHI	GCTAGC	1 cut(s)	833
AvaI	CYCGRG	1 cut(s)	819
AvaII	GGWCC	3 cut(s)	355, 1417, 1778
BaeGI	GKGCMC	1 cut(s)	924
BaeI	ACNNNNGTAYC	2 cut(s)	1893, 1926
BamHI	GGATCC	1 cut(s)	1881
BanI	GGYRCC	2 cut(s)	1261, 1901
BanII	GRGCYC	1 cut(s)	924
BauI	CACGAG	1 cut(s)	702
BbrPI	CACGTG	1 cut(s)	171
Bbv12I	GWGCWC	1 cut(s)	41
BbvCI	CCTCAGC	1 cut(s)	1206
BbvI	GCAGC	7 cut(s)	490, 967, 1025, 1156, 1366, 1369, 1411
BccI	CCATC	5 cut(s)	311, 377, 653, 1306, 1411
BceAI	ACGGC	2 cut(s)	1368, 1740
BciT130I	CCWGG	6 cut(s)	215, 332, 810, 1445, 1611, 1875
BciVI	GTATCC	1 cut(s)	1967
BclI	TGATCA	2 cut(s)	1273, 1771
BcnI	CCSGG	1 cut(s)	1707
BcoDI	GTCTC	1 cut(s)	1805
BcuI	ACTAGT	1 cut(s)	1580
BfaI	CTAG	7 cut(s)	149, 834, 1010, 1380, 1581, 1907, 1952
BfmI	CTRYAG	1 cut(s)	1044
BfuAI	ACCTGC	1 cut(s)	1051
BfuI	GTATCC	1 cut(s)	1967
BisI	GCNGC	9 cut(s)	6, 479, 981, 1014, 1145, 1325, 1355, 1358, 1400
BlpI	GCTNAGC	1 cut(s)	1026
BlsI	GCNGC	9 cut(s)	7, 480, 982, 1015, 1146, 1326, 1356, 1359, 1401
BmcAI	AGTACT	1 cut(s)	1291
Bme1390I	CCNGG	7 cut(s)	215, 332, 810, 1445, 1611, 1707, 1875
Bme18I	GGWCC	3 cut(s)	355, 1417, 1778
BmeT110I	CYCGRG	1 cut(s)	819
BmgBI	CACGTC	1 cut(s)	1629
BmgT120I	GGNCC	6 cut(s)	355, 920, 921, 1417, 1607, 1778
BmiI	GGNNCC	9 cut(s)	424, 922, 1052, 1263, 1419, 1779, 1883, 1903, 1920
BmrFI	CCNGG	7 cut(s)	215, 332, 810, 1445, 1611, 1707, 1875
BmsI	GCATC	4 cut(s)	593, 1100, 1201, 1223
BmtI	GCTAGC	1 cut(s)	837
Bpu10I	CCTNAGC	1 cut(s)	1206
Bpu1102I	GCTNAGC	1 cut(s)	1026
BpuEI	CTTGAG	4 cut(s)	160, 196, 206, 449
BpuMI	CCSGG	1 cut(s)	1707
BsaAI	YACGTR	1 cut(s)	171
BsaBI	GATNNNNATC	1 cut(s)	546
BsaJI	CCNNGG	6 cut(s)	231, 924, 1609, 1706, 1735, 1873
BsaWI	WCCGGW	1 cut(s)	201
BsaXI	ACNNNNNCTCC	2 cut(s)	379, 409
Bsc4I	CCNNNNNNNGG	4 cut(s)	98, 769, 917, 976
Bse118I	RCCGGY	1 cut(s)	98
Bse1I	ACTGG	3 cut(s)	1441, 1570, 1659
Bse3DI	GCAATG	2 cut(s)	1071, 1276
Bse8I	GATNNNNATC	1 cut(s)	546
BseBI	CCWGG	6 cut(s)	215, 332, 810, 1445, 1611, 1875
BseDI	CCNNGG	6 cut(s)	231, 924, 1609, 1706, 1735, 1873
BseGI	GGATG	4 cut(s)	322, 1091, 1216, 1846
BseJI	GATNNNNATC	1 cut(s)	546
BseLI	CCNNNNNNNGG	4 cut(s)	98, 769, 917, 976
BseMI	GCAATG	2 cut(s)	1071, 1276
BseMII	CTCAG	4 cut(s)	281, 1017, 1197, 1307
BseNI	ACTGG	3 cut(s)	1441, 1570, 1659
BseSI	GKGCMC	1 cut(s)	924
BseXI	GCAGC	7 cut(s)	490, 967, 1025, 1156, 1366, 1369, 1411
BseYI	CCCAGC	1 cut(s)	1265
BsgI	GTGCAG	2 cut(s)	1062, 1418
BshFI	GGCC	3 cut(s)	762, 922, 1608
BshNI	GGYRCC	2 cut(s)	1261, 1901
BsiHKAI	GWGCWC	1 cut(s)	41
BsiHKCI	CYCGRG	1 cut(s)	819
BsiSI	CCGG	4 cut(s)	99, 131, 202, 1706
BslFI	GGGAC	4 cut(s)	101, 1098, 1403, 1791
BslI	CCNNNNNNNGG	4 cut(s)	98, 769, 917, 976
BsmAI	GTCTC	1 cut(s)	1805
BsmFI	GGGAC	4 cut(s)	101, 1098, 1403, 1791
BsmI	GAATGC	2 cut(s)	1587, 1818
BsnI	GGCC	3 cut(s)	762, 922, 1608
BsoBI	CYCGRG	1 cut(s)	819
Bsp120I	GGGCCC	1 cut(s)	920
Bsp1286I	GDGCHC	2 cut(s)	41, 924
Bsp143I	GATC	7 cut(s)	547, 636, 1069, 1273, 1525, 1771, 1881
Bsp1720I	GCTNAGC	1 cut(s)	1026
Bsp19I	CCATGG	1 cut(s)	1735
BspACI	CCGC	3 cut(s)	134, 1001, 1325
BspANI	GGCC	3 cut(s)	762, 922, 1608
BspCNI	CTCAG	4 cut(s)	282, 1018, 1198, 1306
BspHI	TCATGA	2 cut(s)	369, 1992
BspLI	GGNNCC	9 cut(s)	424, 922, 1052, 1263, 1419, 1779, 1883, 1903, 1920
BspMAI	CTGCAG	1 cut(s)	1048
BspMI	ACCTGC	1 cut(s)	1051
BspOI	GCTAGC	1 cut(s)	837
BspPI	GGATC	5 cut(s)	542, 644, 1077, 1876, 1889
BspT107I	GGYRCC	2 cut(s)	1261, 1901
BsrDI	GCAATG	2 cut(s)	1071, 1276
BsrFI	RCCGGY	1 cut(s)	98
BsrI	ACTGG	3 cut(s)	1441, 1570, 1659
BssAI	RCCGGY	1 cut(s)	98
BssECI	CCNNGG	6 cut(s)	231, 924, 1609, 1706, 1735, 1873
BssMI	GATC	7 cut(s)	547, 636, 1069, 1273, 1525, 1771, 1881
BssSI	CACGAG	1 cut(s)	702
BssT1I	CCWWGG	1 cut(s)	1735
Bst2BI	CACGAG	1 cut(s)	702
Bst2UI	CCWGG	6 cut(s)	215, 332, 810, 1445, 1611, 1875
Bst4CI	ACNGT	8 cut(s)	59, 93, 241, 275, 1258, 1301, 1501, 1514
Bst6I	CTCTTC	2 cut(s)	626, 860
BstAPI	GCANNNNNTGC	1 cut(s)	1366
BstBAI	YACGTR	1 cut(s)	171
BstC8I	GCNNGC	6 cut(s)	100, 136, 835, 1023, 1062, 1077
BstDEI	CTNAG	7 cut(s)	290, 953, 1026, 1206, 1293, 1636, 1784
BstDSI	CCRYGG	2 cut(s)	924, 1735
BstEII	GGTNACC	1 cut(s)	217
BstENI	CCTNNNNNAGG	1 cut(s)	767
BstF5I	GGATG	4 cut(s)	322, 1091, 1216, 1846
BstHHI	GCGC	1 cut(s)	25
BstKTI	GATC	7 cut(s)	550, 639, 1072, 1276, 1528, 1774, 1884
BstMAI	GTCTC	1 cut(s)	1805
BstMBI	GATC	7 cut(s)	547, 636, 1069, 1273, 1525, 1771, 1881
BstMWI	GCNNNNNNNGC	8 cut(s)	242, 980, 1022, 1027, 1141, 1211, 1330, 1366
BstNI	CCWGG	6 cut(s)	215, 332, 810, 1445, 1611, 1875
BstNSI	RCATGY	2 cut(s)	485, 1091
BstPI	GGTNACC	1 cut(s)	217
BstSCI	CCNGG	7 cut(s)	213, 330, 808, 1443, 1609, 1705, 1873
BstSFI	CTRYAG	1 cut(s)	1044
BstSLI	GKGCMC	1 cut(s)	924
BstV1I	GCAGC	7 cut(s)	490, 967, 1025, 1156, 1366, 1369, 1411
BstX2I	RGATCY	1 cut(s)	1881
BstYI	RGATCY	1 cut(s)	1881
BsuI	GTATCC	1 cut(s)	1967
BsuRI	GGCC	3 cut(s)	762, 922, 1608
BtgI	CCRYGG	2 cut(s)	924, 1735
BtrI	CACGTC	1 cut(s)	1629
BtsCI	GGATG	4 cut(s)	322, 1091, 1216, 1846
BtsI	GCAGTG	3 cut(s)	741, 1842, 1903
BtsIMutI	CAGTG	4 cut(s)	741, 1263, 1842, 1903
BveI	ACCTGC	1 cut(s)	1051
Cac8I	GCNNGC	6 cut(s)	100, 136, 835, 1023, 1062, 1077
CciI	TCATGA	2 cut(s)	369, 1992
CfoI	GCGC	1 cut(s)	25
Cfr10I	RCCGGY	1 cut(s)	98
Cfr13I	GGNCC	6 cut(s)	355, 920, 921, 1417, 1607, 1778
CseI	GACGC	1 cut(s)	1100
CsiI	ACCWGGT	2 cut(s)	213, 808
Csp6I	GTAC	7 cut(s)	211, 287, 776, 806, 1254, 1290, 1902
CviQI	GTAC	7 cut(s)	211, 287, 776, 806, 1254, 1290, 1902
DdeI	CTNAG	7 cut(s)	290, 953, 1026, 1206, 1293, 1636, 1784
DpnI	GATC	7 cut(s)	549, 638, 1071, 1275, 1527, 1773, 1883
DpnII	GATC	7 cut(s)	547, 636, 1069, 1273, 1525, 1771, 1881
DraI	TTTAAA	1 cut(s)	325
DraIII	CACNNNGTG	1 cut(s)	171
Eam1104I	CTCTTC	2 cut(s)	626, 860
EarI	CTCTTC	2 cut(s)	626, 860
Eco130I	CCWWGG	1 cut(s)	1735
Eco24I	GRGCYC	1 cut(s)	924
Eco47I	GGWCC	3 cut(s)	355, 1417, 1778
Eco57I	CTGAAG	1 cut(s)	1506
Eco72I	CACGTG	1 cut(s)	171
Eco88I	CYCGRG	1 cut(s)	819
Eco91I	GGTNACC	1 cut(s)	217
EcoNI	CCTNNNNNAGG	1 cut(s)	767
EcoO109I	RGGNCCY	1 cut(s)	1778
EcoO65I	GGTNACC	1 cut(s)	217
EcoRI	GAATTC	1 cut(s)	786
EcoRII	CCWGG	6 cut(s)	213, 330, 808, 1443, 1609, 1873
EcoT14I	CCWWGG	1 cut(s)	1735
EcoT22I	ATGCAT	2 cut(s)	1093, 1216
EcoT38I	GRGCYC	1 cut(s)	924
ErhI	CCWWGG	1 cut(s)	1735
FalI	AAGNNNNNCTT	2 cut(s)	1014, 1046
FaqI	GGGAC	4 cut(s)	101, 1098, 1403, 1791
FauI	CCCGC	1 cut(s)	127
FauNDI	CATATG	1 cut(s)	303
FbaI	TGATCA	2 cut(s)	1273, 1771
FblI	GTMKAC	2 cut(s)	686, 1496
Fnu4HI	GCNGC	9 cut(s)	6, 479, 981, 1014, 1145, 1325, 1355, 1358, 1400
FokI	GGATG	4 cut(s)	329, 1078, 1223, 1853
FriOI	GRGCYC	1 cut(s)	924
Fsp4HI	GCNGC	9 cut(s)	6, 479, 981, 1014, 1145, 1325, 1355, 1358, 1400
FspBI	CTAG	7 cut(s)	149, 834, 1010, 1380, 1581, 1907, 1952
GlaI	GCGC	1 cut(s)	24
GluI	GCNGC	9 cut(s)	6, 479, 981, 1014, 1145, 1325, 1355, 1358, 1400
GsaI	CCCAGC	1 cut(s)	1269
HaeIII	GGCC	3 cut(s)	762, 922, 1608
HapII	CCGG	4 cut(s)	99, 131, 202, 1706
HgaI	GACGC	1 cut(s)	1100
HhaI	GCGC	1 cut(s)	25
Hin6I	GCGC	1 cut(s)	23
HinP1I	GCGC	1 cut(s)	23
HincII	GTYRAC	2 cut(s)	595, 1040
HindII	GTYRAC	2 cut(s)	595, 1040
HindIII	AAGCTT	1 cut(s)	972
HinfI	GANTC	7 cut(s)	181, 250, 929, 1691, 1789, 1850, 1996
HpaI	GTTAAC	1 cut(s)	595
HpaII	CCGG	4 cut(s)	99, 131, 202, 1706
HphI	GGTGA	2 cut(s)	81, 674
Hpy166II	GTNNAC	7 cut(s)	157, 595, 687, 751, 928, 1040, 1497
Hpy188I	TCNGA	5 cut(s)	232, 1296, 1476, 1525, 1796
Hpy188III	TCNNGA	8 cut(s)	185, 370, 466, 791, 821, 987, 1695, 1993
Hpy8I	GTNNAC	7 cut(s)	157, 595, 687, 751, 928, 1040, 1497
HpyAV	CCTTC	4 cut(s)	368, 593, 1530, 1872
HpyCH4III	ACNGT	8 cut(s)	59, 93, 241, 275, 1258, 1301, 1501, 1514
HpyCH4IV	ACGT	4 cut(s)	170, 339, 1170, 1628
HpyF10VI	GCNNNNNNNGC	8 cut(s)	242, 980, 1022, 1027, 1141, 1211, 1330, 1366
HpyF3I	CTNAG	7 cut(s)	290, 953, 1026, 1206, 1293, 1636, 1784
HpySE526I	ACGT	4 cut(s)	170, 339, 1170, 1628
HspAI	GCGC	1 cut(s)	23
KpnI	GGTACC	1 cut(s)	1905
KroI	GCCGGC	1 cut(s)	98
KroNI	GCCGGC	1 cut(s)	100
Ksp22I	TGATCA	2 cut(s)	1273, 1771
KspAI	GTTAAC	1 cut(s)	595
Kzo9I	GATC	7 cut(s)	547, 636, 1069, 1273, 1525, 1771, 1881
LmnI	GCTCC	4 cut(s)	422, 531, 1330, 1918
Lsp1109I	GCAGC	7 cut(s)	490, 967, 1025, 1156, 1366, 1369, 1411
LweI	GCATC	4 cut(s)	593, 1100, 1201, 1223
MabI	ACCWGGT	2 cut(s)	213, 808
MaeI	CTAG	7 cut(s)	149, 834, 1010, 1380, 1581, 1907, 1952
MaeII	ACGT	4 cut(s)	170, 339, 1170, 1628
MaeIII	GTNAC	7 cut(s)	53, 87, 195, 217, 739, 1301, 1661
MalI	GATC	7 cut(s)	549, 638, 1071, 1275, 1527, 1773, 1883
MboI	GATC	7 cut(s)	547, 636, 1069, 1273, 1525, 1771, 1881
MboII	GAAGA	8 cut(s)	245, 551, 579, 643, 662, 706, 877, 1971
MflI	RGATCY	1 cut(s)	1881
MhlI	GDGCHC	2 cut(s)	41, 924
MluCI	AATT	9 cut(s)	411, 438, 734, 786, 794, 848, 993, 1317, 1537
MlyI	GAGTC	2 cut(s)	923, 2005
MmeI	TCCRAC	1 cut(s)	1531
Mph1103I	ATGCAT	2 cut(s)	1093, 1216
MroNI	GCCGGC	1 cut(s)	98
MslI	CAYNNNNRTG	2 cut(s)	107, 746
MspI	CCGG	4 cut(s)	99, 131, 202, 1706
MspR9I	CCNGG	7 cut(s)	215, 332, 810, 1445, 1611, 1707, 1875
Mva1269I	GAATGC	2 cut(s)	1587, 1818
MvaI	CCWGG	6 cut(s)	215, 332, 810, 1445, 1611, 1875
MwoI	GCNNNNNNNGC	8 cut(s)	242, 980, 1022, 1027, 1141, 1211, 1330, 1366
NaeI	GCCGGC	1 cut(s)	100
NciI	CCSGG	1 cut(s)	1707
NcoI	CCATGG	1 cut(s)	1735
NdeI	CATATG	1 cut(s)	303
NdeII	GATC	7 cut(s)	547, 636, 1069, 1273, 1525, 1771, 1881
NgoMIV	GCCGGC	1 cut(s)	98
NheI	GCTAGC	1 cut(s)	833
NlaIV	GGNNCC	9 cut(s)	424, 922, 1052, 1263, 1419, 1779, 1883, 1903, 1920
NmuCI	GTSAC	2 cut(s)	87, 739
NsiI	ATGCAT	2 cut(s)	1093, 1216
NspI	RCATGY	2 cut(s)	485, 1091
OliI	CACNNNNGTG	1 cut(s)	746
PacI	TTAATTAA	1 cut(s)	441
PaeR7I	CTCGAG	1 cut(s)	819
PagI	TCATGA	2 cut(s)	369, 1992
PctI	GAATGC	2 cut(s)	1587, 1818
PdiI	GCCGGC	1 cut(s)	100
PfeI	GAWTC	5 cut(s)	181, 250, 1691, 1789, 1850
PflMI	CCANNNNNTGG	1 cut(s)	976
PfoI	TCCNGGA	1 cut(s)	330
PkrI	GCNGC	9 cut(s)	7, 480, 982, 1015, 1146, 1326, 1356, 1359, 1401
PleI	GAGTC	2 cut(s)	923, 2004
PmaCI	CACGTG	1 cut(s)	171
PmlI	CACGTG	1 cut(s)	171
PpsI	GAGTC	2 cut(s)	923, 2004
Ppu21I	YACGTR	1 cut(s)	171
PpuMI	RGGWCCY	1 cut(s)	1778
Psp1406I	AACGTT	1 cut(s)	1170
Psp5II	RGGWCCY	1 cut(s)	1778
Psp6I	CCWGG	6 cut(s)	213, 330, 808, 1443, 1609, 1873
PspCI	CACGTG	1 cut(s)	171
PspEI	GGTNACC	1 cut(s)	217
PspFI	CCCAGC	1 cut(s)	1265
PspGI	CCWGG	6 cut(s)	213, 330, 808, 1443, 1609, 1873
PspN4I	GGNNCC	9 cut(s)	424, 922, 1052, 1263, 1419, 1779, 1883, 1903, 1920
PspOMI	GGGCCC	1 cut(s)	920
PspPI	GGNCC	6 cut(s)	355, 920, 921, 1417, 1607, 1778
PspPPI	RGGWCCY	1 cut(s)	1778
PsrI	GAACNNNNNNTAC	2 cut(s)	43, 75
PstI	CTGCAG	1 cut(s)	1048
PsuI	RGATCY	1 cut(s)	1881
RsaI	GTAC	7 cut(s)	212, 288, 777, 807, 1255, 1291, 1903
RsaNI	GTAC	7 cut(s)	211, 287, 776, 806, 1254, 1290, 1902
RseI	CAYNNNNRTG	2 cut(s)	107, 746
SatI	GCNGC	9 cut(s)	6, 479, 981, 1014, 1145, 1325, 1355, 1358, 1400
Sau3AI	GATC	7 cut(s)	547, 636, 1069, 1273, 1525, 1771, 1881
Sau96I	GGNCC	6 cut(s)	355, 920, 921, 1417, 1607, 1778
SbfI	CCTGCAGG	1 cut(s)	1048
ScaI	AGTACT	1 cut(s)	1291
SchI	GAGTC	2 cut(s)	923, 2005
ScrFI	CCNGG	7 cut(s)	215, 332, 810, 1445, 1611, 1707, 1875
SdaI	CCTGCAGG	1 cut(s)	1048
SduI	GDGCHC	2 cut(s)	41, 924
SexAI	ACCWGGT	2 cut(s)	213, 808
SfaNI	GCATC	4 cut(s)	593, 1100, 1201, 1223
SfcI	CTRYAG	1 cut(s)	1044
Sfr274I	CTCGAG	1 cut(s)	819
SinI	GGWCC	3 cut(s)	355, 1417, 1778
SlaI	CTCGAG	1 cut(s)	819
SmiMI	CAYNNNNRTG	2 cut(s)	107, 746
SmlI	CTYRAG	5 cut(s)	139, 175, 185, 464, 819
SmoI	CTYRAG	5 cut(s)	139, 175, 185, 464, 819
SpeI	ACTAGT	1 cut(s)	1580
Sse8387I	CCTGCAGG	1 cut(s)	1048
Sse9I	AATT	9 cut(s)	411, 438, 734, 786, 794, 848, 993, 1317, 1537
SsiI	CCGC	3 cut(s)	134, 1001, 1325
SspI	AATATT	3 cut(s)	472, 1965, 1976
SspMI	CTAG	7 cut(s)	149, 834, 1010, 1380, 1581, 1907, 1952
StyD4I	CCNGG	7 cut(s)	213, 330, 808, 1443, 1609, 1705, 1873
StyI	CCWWGG	1 cut(s)	1735
TaaI	ACNGT	8 cut(s)	59, 93, 241, 275, 1258, 1301, 1501, 1514
TaiI	ACGT	4 cut(s)	173, 342, 1173, 1631
TaqI	TCGA	3 cut(s)	692, 820, 1828
TasI	AATT	9 cut(s)	411, 438, 734, 786, 794, 848, 993, 1317, 1537
TatI	WGTACW	2 cut(s)	1253, 1289
TauI	GCSGC	1 cut(s)	1327
TfiI	GAWTC	5 cut(s)	181, 250, 1691, 1789, 1850
TscAI	CASTG	4 cut(s)	748, 1263, 1842, 1903
TseFI	GTSAC	2 cut(s)	87, 739
TseI	GCWGC	8 cut(s)	5, 478, 980, 1013, 1144, 1354, 1357, 1399
Tsp45I	GTSAC	2 cut(s)	87, 739
TspDTI	ATGAA	5 cut(s)	603, 815, 1129, 1377, 1981
TspGWI	ACGGA	1 cut(s)	1183
TspRI	CASTG	4 cut(s)	748, 1263, 1842, 1903
Van91I	CCANNNNNTGG	1 cut(s)	976
VpaK11BI	GGWCC	3 cut(s)	355, 1417, 1778
XagI	CCTNNNNNAGG	1 cut(s)	767
XapI	RAATTY	3 cut(s)	786, 848, 993
XceI	RCATGY	2 cut(s)	485, 1091
XhoI	CTCGAG	1 cut(s)	819
XmiI	GTMKAC	2 cut(s)	686, 1496
XspI	CTAG	7 cut(s)	149, 834, 1010, 1380, 1581, 1907, 1952
ZrmI	AGTACT	1 cut(s)	1291
Zsp2I	ATGCAT	2 cut(s)	1093, 1216

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.