Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0015432

Physical Location & Seq

Genomic Coordinates Forward (+)

1306222 .. 1309184

Gene Structure 2963 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1020900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1437 bp

ATGGCAGCTTCCAAGATGTTATTGGCTTCCTTCACCGCCTTCCGCCCTCTAACTTGCGCTGCCTCCGCCTCTTATCCTGCGCGCTTGGCCCCACACCCGCCGGACCTGATCAAGTGGGTCCGTCGGGAAGGAGGATTTGTGCACGAGGGCGTGAAGATAGCTCAAGACAGTTCCTTTGGTCTTGGATTGGTGGCTTCTGAGGAAATCCCAAAGGGTTCCGAACTAATTGTCCTTCCGGAGCACGTACCTTTGAGATTTGGCTTAACTGAATCGGAAGGCGGGGATGGGGTCGACTCTGTTCTCGACAATTTGGCTCGCCAAGTTCCTGAGGAACTATGGGCAATGAAATTGGGTTTGAAGCTTCTGCAAGAAAGAGCAAAAACTGGATCCTTCTGGTGGCCATACATCAGCAGCCTCCCTGAAACGTTCAATATACCCATCTTCTTCCCTGGCGAGGACATAAAGAATTTGCAGTATGCTCCACTTCTTTACCAGGTAAACAAGAGATGCAGATTTCTTCTTGATTTTGAACAAGAAGTCAAGCGTGCTCTGGAAAATCTGAAACCAAATGATCACCCTTTTGGTGGCCAAGCTGTAGATGCATCTTCTCTTGGATGGGCAATGTCAGCTGTTTCGTCTCGGGCATTCCGTTTATATGGTAGAAAACTTCCAGGCGGGAACTACAATGATATTCCGATGATGCTTCCTCTCATCGATATGTGCAACCACAGTTTCAACCCAAATGCTGCAATAGTTCAGGACCAAGATGACAGGAATATGAAGATGCTAGTAAAGGTTGTAGCAGAAACAGAGATCAAGGAGAACGATGGTTTAGTACTCAACTATGGATGTTTAAATAATGATCTTTTCCTTCTGGATTATGGATTTGTGATACCTTCAAACCCCTATGATTGTATTGAACTCAAATATGATGGAGCACTTCTGGATGCTGCAAGTATGGCTGCCGGAGTATCATCGCCCAACTTCTCTGCACCATCTCCATGGCAGAAAGAAATCTTATGCCAGCTAAAGTTAGATGGAGAAGCTCTGCTTCTTAAGGTATGCTTAGGGGGCTCAGAACTGGTTGAGGAGCGCTTGTTGGCAGCCTTAAGAGTTGTTCTGGCTGATGACAAAGAAACAGTTCAGAAGCACGATTTAAACAAACTTAAATCTATATCAGCTGAAGCTCCCCTTGGTATTGCAAATGAATTAGCTGCTTTTCGCACCGTTATTGCATTATGTGTGATTGCACTGGGACATTTCCCAACCAAAATCATGGAAGACGAATCCCTGTTGAGTAAAGGTGTTTCAGATTCTACTCAGCTGGCTATCCAATTCAGAATTCATAAAAAATCGGTGATTATAGACGTGATGAGAGATCTCACAAAGAGGGTGAAGTTTCTTGCATCAAAAGAAACCGTCGCCACTAAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003712 GO:0003713 GO:0003824 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010452 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018023 GO:0018024 GO:0018026 GO:0018027 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032259 GO:0034968 GO:0036211 GO:0042054 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045893 GO:0045935 GO:0046975 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:0140110 GO:1901564 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rubis-subs-bind SET

Protein Analysis

479

Amino Acids

52.68

Weight (kDa)

5.64

Isoelectric Point (pI)

40.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SET	PF00856	60 - 283	1.2e-06	SET domain
Rubis-subs-bind	PF09273	334 - 453	2.3e-17	Rubisco LSMT substrate-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015432)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24610
fragaria_vesca	FvH4_4g33660
malus_domestica	MD13G1020900.v1.1 MD16G1019300.v1.1
prunus_persica	Prupe.1G332800_v2.0.a1
pyrus_communis	pycom13g01770 pycom16g01730
rosa_chinensis	RchiOBHm_Chr4g0443121
rosa_laevigata	RLG00000005952
rosa_multiflora	Rmu_sc0007968.1_g000013
rosa_roxburghii	Rroxscaffold_5G00383740
rosa_rugosa	Rorug04G0344600
rosa_samantha	Rh4BG410500 Rh4CG426000 Rh4DG405500
rosa_wichuraiana	Rw4G034390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	291
AccII	CGCG	1 cut(s)	82
AccIII	TCCGGA	1 cut(s)	235
AciI	CCGC	6 cut(s)	36, 43, 66, 98, 279, 675
AclI	AACGTT	1 cut(s)	425
AclWI	GGATC	2 cut(s)	381, 394
AcoI	YGGCCR	2 cut(s)	398, 586
AcsI	RAATTY	2 cut(s)	466, 1341
AcuI	CTGAAG	1 cut(s)	1203
AfaI	GTAC	2 cut(s)	246, 837
AfeI	AGCGCT	1 cut(s)	1094
AfiI	CCNNNNNNNGG	3 cut(s)	396, 454, 584
AflII	CTTAAG	2 cut(s)	1055, 1108
AgsI	TTSAA	6 cut(s)	358, 430, 530, 736, 900, 920
AjiI	CACGTC	1 cut(s)	1369
AjnI	CCWGG	3 cut(s)	448, 492, 670
AjuI	GAANNNNNNNTTGG	4 cut(s)	1176, 1208, 1326, 1358
AloI	GAACNNNNNNTCC	2 cut(s)	213, 245
Alw21I	GWGCWC	4 cut(s)	144, 243, 550, 940
Alw26I	GTCTC	1 cut(s)	642
Alw44I	GTGCAC	1 cut(s)	140
AlwI	GGATC	2 cut(s)	381, 394
Ama87I	CYCGRG	1 cut(s)	639
Aor13HI	TCCGGA	1 cut(s)	235
Aor51HI	AGCGCT	1 cut(s)	1094
AoxI	GGCC	3 cut(s)	87, 398, 586
ApaLI	GTGCAC	1 cut(s)	140
ApeKI	GCWGC	8 cut(s)	5, 59, 411, 746, 950, 962, 1103, 1214
ApoI	RAATTY	2 cut(s)	466, 1341
ArsI	GACNNNNNNTTYG	4 cut(s)	158, 190, 213, 245
Asp700I	GAANNNNTTC	1 cut(s)	1140
AspLEI	GCGC	4 cut(s)	59, 82, 84, 1095
AspS9I	GGNCC	4 cut(s)	88, 103, 118, 760
AsuHPI	GGTGA	4 cut(s)	25, 566, 1369, 1405
AvaI	CYCGRG	1 cut(s)	639
AvaII	GGWCC	3 cut(s)	103, 118, 760
AxyI	CCTNAGG	1 cut(s)	327
BaeGI	GKGCMC	1 cut(s)	144
BalI	TGGCCA	2 cut(s)	400, 588
BamHI	GGATCC	1 cut(s)	386
BanII	GRGCYC	1 cut(s)	1076
BauI	CACGAG	1 cut(s)	143
BbsI	GAAGAC	1 cut(s)	1287
Bbv12I	GWGCWC	4 cut(s)	144, 243, 550, 940
BbvI	GCAGC	8 cut(s)	17, 46, 423, 733, 937, 949, 1115, 1201
BccI	CCATC	7 cut(s)	278, 446, 609, 821, 926, 1003, 1031
BciT130I	CCWGG	3 cut(s)	450, 494, 672
BclI	TGATCA	2 cut(s)	108, 571
BcoDI	GTCTC	1 cut(s)	642
BfaI	CTAG	1 cut(s)	788
BfmI	CTRYAG	1 cut(s)	594
BfoI	RGCGCY	1 cut(s)	1096
BfrI	CTTAAG	2 cut(s)	1055, 1108
BglII	AGATCT	1 cut(s)	1378
BisI	GCNGC	8 cut(s)	6, 60, 412, 747, 951, 963, 1104, 1215
BlsI	GCNGC	8 cut(s)	7, 61, 413, 748, 952, 964, 1105, 1216
BmcAI	AGTACT	1 cut(s)	837
Bme1390I	CCNGG	3 cut(s)	450, 494, 672
Bme18I	GGWCC	3 cut(s)	103, 118, 760
BmeT110I	CYCGRG	1 cut(s)	639
BmgBI	CACGTC	1 cut(s)	1369
BmgT120I	GGNCC	4 cut(s)	88, 103, 118, 760
BmiI	GGNNCC	4 cut(s)	90, 119, 217, 388
BmrFI	CCNGG	3 cut(s)	450, 494, 672
BmrI	ACTGGG	1 cut(s)	1262
BmsI	GCATC	7 cut(s)	497, 589, 611, 690, 774, 937, 1415
BmuI	ACTGGG	1 cut(s)	1262
BpiI	GAAGAC	1 cut(s)	1287
Bpu10I	CCTNAGC	1 cut(s)	1066
BpuEI	CTTGAG	1 cut(s)	147
Bsa29I	ATCGAT	1 cut(s)	714
BsaAI	YACGTR	1 cut(s)	244
BsaJI	CCNNGG	3 cut(s)	448, 1001, 1192
BsaWI	WCCGGW	1 cut(s)	235
BsaXI	ACNNNNNCTCC	4 cut(s)	123, 153, 1082, 1112
Bsc4I	CCNNNNNNNGG	3 cut(s)	396, 454, 584
Bse1I	ACTGG	3 cut(s)	388, 1086, 1257
Bse21I	CCTNAGG	1 cut(s)	327
Bse3DI	GCAATG	2 cut(s)	348, 627
BseAI	TCCGGA	1 cut(s)	235
BseBI	CCWGG	3 cut(s)	450, 494, 672
BseCI	ATCGAT	1 cut(s)	714
BseDI	CCNNGG	3 cut(s)	448, 1001, 1192
BseGI	GGATG	4 cut(s)	289, 620, 854, 952
BseLI	CCNNNNNNNGG	3 cut(s)	396, 454, 584
BseMI	GCAATG	2 cut(s)	348, 627
BseMII	CTCAG	4 cut(s)	189, 318, 1089, 1334
BseNI	ACTGG	3 cut(s)	388, 1086, 1257
BsePI	GCGCGC	1 cut(s)	80
BseRI	GAGGAG	1 cut(s)	1103
BseSI	GKGCMC	1 cut(s)	144
BseXI	GCAGC	8 cut(s)	17, 46, 423, 733, 937, 949, 1115, 1201
BsgI	GTGCAG	1 cut(s)	975
Bsh1236I	CGCG	1 cut(s)	82
BshFI	GGCC	3 cut(s)	89, 400, 588
BshVI	ATCGAT	1 cut(s)	714
BsiHKAI	GWGCWC	4 cut(s)	144, 243, 550, 940
BsiHKCI	CYCGRG	1 cut(s)	639
BsiSI	CCGG	3 cut(s)	101, 236, 966
BslFI	GGGAC	1 cut(s)	1269
BslI	CCNNNNNNNGG	3 cut(s)	396, 454, 584
BsmAI	GTCTC	1 cut(s)	642
BsmBI	CGTCTC	1 cut(s)	642
BsmFI	GGGAC	1 cut(s)	1269
BsmI	GAATGC	1 cut(s)	644
BsnI	GGCC	3 cut(s)	89, 400, 588
BsoBI	CYCGRG	1 cut(s)	639
Bsp1286I	GDGCHC	5 cut(s)	144, 243, 550, 940, 1076
Bsp13I	TCCGGA	1 cut(s)	235
Bsp143I	GATC	6 cut(s)	108, 386, 571, 813, 862, 1378
Bsp19I	CCATGG	1 cut(s)	1001
BspACI	CCGC	6 cut(s)	36, 43, 66, 98, 279, 675
BspANI	GGCC	3 cut(s)	89, 400, 588
BspCNI	CTCAG	4 cut(s)	190, 319, 1088, 1333
BspDI	ATCGAT	1 cut(s)	714
BspEI	TCCGGA	1 cut(s)	235
BspFNI	CGCG	1 cut(s)	82
BspLI	GGNNCC	4 cut(s)	90, 119, 217, 388
BspPI	GGATC	2 cut(s)	381, 394
BspTI	CTTAAG	2 cut(s)	1055, 1108
BsrDI	GCAATG	2 cut(s)	348, 627
BsrI	ACTGG	3 cut(s)	388, 1086, 1257
BssECI	CCNNGG	3 cut(s)	448, 1001, 1192
BssHII	GCGCGC	1 cut(s)	80
BssMI	GATC	6 cut(s)	108, 386, 571, 813, 862, 1378
BssSI	CACGAG	1 cut(s)	143
BssT1I	CCWWGG	2 cut(s)	1001, 1192
Bst2BI	CACGAG	1 cut(s)	143
Bst2UI	CCWGG	3 cut(s)	450, 494, 672
Bst4CI	ACNGT	5 cut(s)	170, 731, 1141, 1228, 1420
BstAFI	CTTAAG	2 cut(s)	1055, 1108
BstBAI	YACGTR	1 cut(s)	244
BstC8I	GCNNGC	5 cut(s)	82, 316, 546, 1025, 1326
BstDEI	CTNAG	5 cut(s)	198, 327, 1066, 1075, 1320
BstDSI	CCRYGG	1 cut(s)	1001
BstF5I	GGATG	4 cut(s)	289, 620, 854, 952
BstFNI	CGCG	1 cut(s)	82
BstH2I	RGCGCY	1 cut(s)	1096
BstHHI	GCGC	4 cut(s)	59, 82, 84, 1095
BstKTI	GATC	6 cut(s)	111, 389, 574, 816, 865, 1381
BstMAI	GTCTC	1 cut(s)	642
BstMBI	GATC	6 cut(s)	108, 386, 571, 813, 862, 1378
BstMWI	GCNNNNNNNGC	6 cut(s)	65, 86, 599, 626, 959, 1071
BstNI	CCWGG	3 cut(s)	450, 494, 672
BstSCI	CCNGG	3 cut(s)	448, 492, 670
BstSFI	CTRYAG	1 cut(s)	594
BstSLI	GKGCMC	1 cut(s)	144
BstUI	CGCG	1 cut(s)	82
BstV1I	GCAGC	8 cut(s)	17, 46, 423, 733, 937, 949, 1115, 1201
BstV2I	GAAGAC	1 cut(s)	1287
BstX2I	RGATCY	2 cut(s)	386, 1378
BstXI	CCANNNNNNTGG	2 cut(s)	1002, 1276
BstYI	RGATCY	2 cut(s)	386, 1378
Bsu15I	ATCGAT	1 cut(s)	714
Bsu36I	CCTNAGG	1 cut(s)	327
BsuRI	GGCC	3 cut(s)	89, 400, 588
BsuTUI	ATCGAT	1 cut(s)	714
BtgI	CCRYGG	1 cut(s)	1001
BtgZI	GCGATG	1 cut(s)	960
BtrI	CACGTC	1 cut(s)	1369
BtsCI	GGATG	4 cut(s)	289, 620, 854, 952
BtsIMutI	CAGTG	1 cut(s)	1250
Cac8I	GCNNGC	5 cut(s)	82, 316, 546, 1025, 1326
CfoI	GCGC	4 cut(s)	59, 82, 84, 1095
Cfr13I	GGNCC	4 cut(s)	88, 103, 118, 760
ClaI	ATCGAT	1 cut(s)	714
CsiI	ACCWGGT	1 cut(s)	492
Csp6I	GTAC	2 cut(s)	245, 836
CviAII	CATG	2 cut(s)	1002, 1276
CviQI	GTAC	2 cut(s)	245, 836
DdeI	CTNAG	5 cut(s)	198, 327, 1066, 1075, 1320
DpnI	GATC	6 cut(s)	110, 388, 573, 815, 864, 1380
DpnII	GATC	6 cut(s)	108, 386, 571, 813, 862, 1378
DraI	TTTAAA	2 cut(s)	855, 1158
EaeI	YGGCCR	2 cut(s)	398, 586
EciI	GGCGGA	2 cut(s)	32, 55
Eco130I	CCWWGG	2 cut(s)	1001, 1192
Eco24I	GRGCYC	1 cut(s)	1076
Eco47I	GGWCC	3 cut(s)	103, 118, 760
Eco47III	AGCGCT	1 cut(s)	1094
Eco57I	CTGAAG	1 cut(s)	1203
Eco81I	CCTNAGG	1 cut(s)	327
Eco88I	CYCGRG	1 cut(s)	639
EcoRI	GAATTC	1 cut(s)	1341
EcoRII	CCWGG	3 cut(s)	448, 492, 670
EcoT14I	CCWWGG	2 cut(s)	1001, 1192
EcoT22I	ATGCAT	1 cut(s)	604
EcoT38I	GRGCYC	1 cut(s)	1076
ErhI	CCWWGG	2 cut(s)	1001, 1192
Esp3I	CGTCTC	1 cut(s)	642
FaeI	CATG	2 cut(s)	1005, 1279
FalI	AAGNNNNNCTT	6 cut(s)	1035, 1067, 1049, 1081, 1176, 1208
FaqI	GGGAC	1 cut(s)	1269
FatI	CATG	2 cut(s)	1001, 1275
FauI	CCCGC	3 cut(s)	105, 272, 668
FbaI	TGATCA	2 cut(s)	108, 571
FblI	GTMKAC	1 cut(s)	291
Fnu4HI	GCNGC	8 cut(s)	6, 60, 412, 747, 951, 963, 1104, 1215
FokI	GGATG	4 cut(s)	296, 627, 861, 959
FriOI	GRGCYC	1 cut(s)	1076
Fsp4HI	GCNGC	8 cut(s)	6, 60, 412, 747, 951, 963, 1104, 1215
FspBI	CTAG	1 cut(s)	788
GlaI	GCGC	4 cut(s)	58, 81, 83, 1094
GluI	GCNGC	8 cut(s)	6, 60, 412, 747, 951, 963, 1104, 1215
HaeII	RGCGCY	1 cut(s)	1096
HaeIII	GGCC	3 cut(s)	89, 400, 588
HapII	CCGG	3 cut(s)	101, 236, 966
HhaI	GCGC	4 cut(s)	59, 82, 84, 1095
Hin1II	CATG	2 cut(s)	1005, 1279
Hin6I	GCGC	4 cut(s)	57, 80, 82, 1093
HinP1I	GCGC	4 cut(s)	57, 80, 82, 1093
HincII	GTYRAC	1 cut(s)	292
HindII	GTYRAC	1 cut(s)	292
HindIII	AAGCTT	1 cut(s)	359
HinfI	GANTC	4 cut(s)	269, 293, 1286, 1313
HpaII	CCGG	3 cut(s)	101, 236, 966
HphI	GGTGA	4 cut(s)	25, 566, 1369, 1405
Hpy166II	GTNNAC	3 cut(s)	142, 292, 499
Hpy188I	TCNGA	9 cut(s)	199, 220, 274, 561, 696, 1078, 1146, 1312, 1340
Hpy8I	GTNNAC	3 cut(s)	142, 292, 499
Hpy99I	CGWCG	2 cut(s)	126, 1424
HpyAV	CCTTC	8 cut(s)	40, 49, 122, 242, 269, 400, 881, 906
HpyCH4III	ACNGT	5 cut(s)	170, 731, 1141, 1228, 1420
HpyCH4IV	ACGT	3 cut(s)	243, 425, 1368
HpyF10VI	GCNNNNNNNGC	6 cut(s)	65, 86, 599, 626, 959, 1071
HpyF3I	CTNAG	5 cut(s)	198, 327, 1066, 1075, 1320
HpySE526I	ACGT	3 cut(s)	243, 425, 1368
Hsp92II	CATG	2 cut(s)	1005, 1279
HspAI	GCGC	4 cut(s)	57, 80, 82, 1093
Kpn2I	TCCGGA	1 cut(s)	235
Ksp22I	TGATCA	2 cut(s)	108, 571
Kzo9I	GATC	6 cut(s)	108, 386, 571, 813, 862, 1378
LmnI	GCTCC	5 cut(s)	238, 484, 935, 1090, 1192
Lsp1109I	GCAGC	8 cut(s)	17, 46, 423, 733, 937, 949, 1115, 1201
LweI	GCATC	7 cut(s)	497, 589, 611, 690, 774, 937, 1415
MabI	ACCWGGT	1 cut(s)	492
MaeI	CTAG	1 cut(s)	788
MaeII	ACGT	3 cut(s)	243, 425, 1368
MalI	GATC	6 cut(s)	110, 388, 573, 815, 864, 1380
MboI	GATC	6 cut(s)	108, 386, 571, 813, 862, 1378
MboII	GAAGA	7 cut(s)	166, 433, 436, 509, 597, 793, 1292
MflI	RGATCY	2 cut(s)	386, 1378
MhlI	GDGCHC	5 cut(s)	144, 243, 550, 940, 1076
MlsI	TGGCCA	2 cut(s)	400, 588
MluCI	AATT	7 cut(s)	225, 307, 347, 466, 1208, 1334, 1341
MluNI	TGGCCA	2 cut(s)	400, 588
MlyI	GAGTC	1 cut(s)	287
Mox20I	TGGCCA	2 cut(s)	400, 588
Mph1103I	ATGCAT	1 cut(s)	604
MroI	TCCGGA	1 cut(s)	235
MroXI	GAANNNNTTC	1 cut(s)	1140
MscI	TGGCCA	2 cut(s)	400, 588
MseI	TTAA	6 cut(s)	263, 854, 1056, 1109, 1157, 1167
MslI	CAYNNNNRTG	2 cut(s)	716, 1000
Msp20I	TGGCCA	2 cut(s)	400, 588
MspA1I	CMGCKG	3 cut(s)	629, 1181, 1324
MspCI	CTTAAG	2 cut(s)	1055, 1108
MspI	CCGG	3 cut(s)	101, 236, 966
MspR9I	CCNGG	3 cut(s)	450, 494, 672
Mva1269I	GAATGC	1 cut(s)	644
MvaI	CCWGG	3 cut(s)	450, 494, 672
MvnI	CGCG	1 cut(s)	82
MwoI	GCNNNNNNNGC	6 cut(s)	65, 86, 599, 626, 959, 1071
NcoI	CCATGG	1 cut(s)	1001
NdeII	GATC	6 cut(s)	108, 386, 571, 813, 862, 1378
NlaIII	CATG	2 cut(s)	1005, 1279
NlaIV	GGNNCC	4 cut(s)	90, 119, 217, 388
NsiI	ATGCAT	1 cut(s)	604
PauI	GCGCGC	1 cut(s)	80
PcsI	WCGNNNNNNNCGW	1 cut(s)	646
PctI	GAATGC	1 cut(s)	644
PdmI	GAANNNNTTC	1 cut(s)	1140
PfeI	GAWTC	3 cut(s)	269, 1286, 1313
PkrI	GCNGC	8 cut(s)	7, 61, 413, 748, 952, 964, 1105, 1216
PleI	GAGTC	1 cut(s)	287
PpsI	GAGTC	1 cut(s)	287
Ppu21I	YACGTR	1 cut(s)	244
Psp1406I	AACGTT	1 cut(s)	425
Psp6I	CCWGG	3 cut(s)	448, 492, 670
PspGI	CCWGG	3 cut(s)	448, 492, 670
PspN4I	GGNNCC	4 cut(s)	90, 119, 217, 388
PspPI	GGNCC	4 cut(s)	88, 103, 118, 760
PsuI	RGATCY	2 cut(s)	386, 1378
PteI	GCGCGC	1 cut(s)	80
PvuII	CAGCTG	3 cut(s)	629, 1181, 1324
RsaI	GTAC	2 cut(s)	246, 837
RsaNI	GTAC	2 cut(s)	245, 836
RseI	CAYNNNNRTG	2 cut(s)	716, 1000
SalI	GTCGAC	1 cut(s)	290
SaqAI	TTAA	6 cut(s)	263, 854, 1056, 1109, 1157, 1167
SatI	GCNGC	8 cut(s)	6, 60, 412, 747, 951, 963, 1104, 1215
Sau3AI	GATC	6 cut(s)	108, 386, 571, 813, 862, 1378
Sau96I	GGNCC	4 cut(s)	88, 103, 118, 760
ScaI	AGTACT	1 cut(s)	837
SchI	GAGTC	1 cut(s)	287
ScrFI	CCNGG	3 cut(s)	450, 494, 672
SduI	GDGCHC	5 cut(s)	144, 243, 550, 940, 1076
SexAI	ACCWGGT	1 cut(s)	492
SfaNI	GCATC	7 cut(s)	497, 589, 611, 690, 774, 937, 1415
SfcI	CTRYAG	1 cut(s)	594
SinI	GGWCC	3 cut(s)	103, 118, 760
SmiMI	CAYNNNNRTG	2 cut(s)	716, 1000
SmlI	CTYRAG	3 cut(s)	162, 1055, 1108
SmoI	CTYRAG	3 cut(s)	162, 1055, 1108
Sse9I	AATT	7 cut(s)	225, 307, 347, 466, 1208, 1334, 1341
SsiI	CCGC	6 cut(s)	36, 43, 66, 98, 279, 675
SspMI	CTAG	1 cut(s)	788
StyD4I	CCNGG	3 cut(s)	448, 492, 670
StyI	CCWWGG	2 cut(s)	1001, 1192
TaaI	ACNGT	5 cut(s)	170, 731, 1141, 1228, 1420
TaiI	ACGT	3 cut(s)	246, 428, 1371
TaqI	TCGA	3 cut(s)	291, 303, 714
TasI	AATT	7 cut(s)	225, 307, 347, 466, 1208, 1334, 1341
TatI	WGTACW	1 cut(s)	835
TfiI	GAWTC	3 cut(s)	269, 1286, 1313
Tru1I	TTAA	6 cut(s)	263, 854, 1056, 1109, 1157, 1167
Tru9I	TTAA	6 cut(s)	263, 854, 1056, 1109, 1157, 1167
TscAI	CASTG	1 cut(s)	1257
TseI	GCWGC	8 cut(s)	5, 59, 411, 746, 950, 962, 1103, 1214
TspDTI	ATGAA	4 cut(s)	359, 794, 1221, 1334
TspGWI	ACGGA	2 cut(s)	110, 638
TspRI	CASTG	1 cut(s)	1257
Vha464I	CTTAAG	2 cut(s)	1055, 1108
VneI	GTGCAC	1 cut(s)	140
VpaK11BI	GGWCC	3 cut(s)	103, 118, 760
XapI	RAATTY	2 cut(s)	466, 1341
XcmI	CCANNNNNNNNNTGG	1 cut(s)	19
XmiI	GTMKAC	1 cut(s)	291
XmnI	GAANNNNTTC	1 cut(s)	1140
XspI	CTAG	1 cut(s)	788
ZrmI	AGTACT	1 cut(s)	837
Zsp2I	ATGCAT	1 cut(s)	604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.