MD13G1047100.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012088
Physical Location & Seq
Reverse (-)
3285606 .. 3288877
3272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1047100.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGATGTGTGGGAAGAAAGAAGATCAAGGGGAGTGCTCTCAGTCAGTCCAAAACCTACAAGCTTTCCAAGAACACTTGTTTCTCCAACAACAACAACATCATCAGATGCAACAACAACAACATCATCAGATGCAACAACAACAACATCATCAGATGCAACAACAACAAGGTTTGATTTTTCCACATGATCATCAACCTCCTCCTCCAATCTTGCAGCCATGGACTCTCCCACCGAACCACACCTTCAACCCAACCGCCCACGACCCATTTCTCCTCCCAACTCCTCCCCCAATTCCGTCGTCATCATCCTATGCTTCTTTCTTCAACCGAAGAGCTCCTTCTCTCCAGTTCACATACGACGGTTCAACATCTGACCACCACCACCACCTCAGAATTTTGTCCGAGACGCTTGGACCGATGGTTCAACCCGGTTCGGCCCCTTTCGGTCTTCAGGCCGAGTTAGGAAAGTTGACCGCCCAAGAAATCATGGACGCCAAGGCACTCGCCGCTTCTAAGAGTCACAGCGAGGCTGAGAGGAGACGAAGAGAGAGAATCAACAACCATCTCGCCAAGTTGCGCAGCTTATTGCCCAGCACCACCAAAACGGACAAAGCTTCATTGCTAGCGGAAGTGATACAACATGTGAAAGAGCTGAAACGCCAAACTTCTCTGATCGCCGAGACGAGTCCGGTACCGACCGAAACTGACGAACTAATAGTAGACGATGAATCTGACGAGGACGGTAAGTTTGTGATAAAGGCCTCACTTTGCTGCGAGGACCGATCGGATCTCTTGCCCGACCTAATCAAGACATTGAAAGCCTTGCGCTTGAGAACGCTCAAAGCTGAGATCACAACACTCGGCGGACGTGTGAAGAACGTGTTGTTTATCACCGGAGAAGAGGACTCAAGTAATAGCGGAGGGGAGCAAATACAAATGCCGCATCAGCAGTACTGCAAAAGTTCGATACAAGAAGCGCTGAAGGCGGTCATGGAGAAGACCGCCGGCGGTGGTGGTGATCAGGATCAGTCTTCCTCAGGGAGTGCTAAGAGACAAAGGACTAATAACATCAATATCCTTGAACACAGGTCGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

366

Amino Acids

40.88

Weight (kDa)

6.46

Isoelectric Point (pI)

67.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 172 - 219 9e-13 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68810
fragaria_vesca FvH4_4g28130 FvH4_4g28130
malus_domestica MD13G1047100.v1.1 MD16G1048700.v1.1
prunus_persica Prupe.1G304900_v2.0.a1
pyrus_communis pycom13g04120 pycom16g04260
rosa_chinensis RchiOBHm_Chr4g0435901
rosa_laevigata RLG00000006551
rosa_multiflora Rmu_sc0003165.1_g000008
rosa_roxburghii Rroxscaffold_5G00377070
rosa_rugosa Rorug04G0290700 Rorug04G0290700
rosa_samantha Rh4AG344900 Rh4BG353700 Rh4CG368100 Rh4DG347500
rosa_wichuraiana Rw4G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 578
Acc65I GGTACC 1 cut(s) 691
AccB1I GGYRCC 1 cut(s) 691
AccI GTMKAC 1 cut(s) 720
AclWI GGATC 2 cut(s) 795, 1032
AcsI RAATTY 1 cut(s) 393
AcuI CTGAAG 2 cut(s) 434, 1001
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 2 cut(s) 693, 953
AfeI AGCGCT 1 cut(s) 978
AflIII ACRYGT 3 cut(s) 640, 868, 879
AgsI TTSAA 6 cut(s) 247, 325, 366, 425, 817, 1082
AjiI CACGTC 1 cut(s) 869
AloI GAACNNNNNNTCC 2 cut(s) 405, 437
AluBI AGCT 6 cut(s) 62, 335, 582, 614, 652, 845
AluI AGCT 6 cut(s) 62, 335, 582, 614, 652, 845
Alw21I GWGCWC 2 cut(s) 38, 337
Alw26I GTCTC 4 cut(s) 398, 532, 674, 1045
AlwI GGATC 2 cut(s) 795, 1032
Aor51HI AGCGCT 1 cut(s) 978
AoxI GGCC 3 cut(s) 435, 453, 759
ApeKI GCWGC 3 cut(s) 214, 579, 771
ApoI RAATTY 1 cut(s) 393
ArsI GACNNNNNNTTYG 2 cut(s) 731, 763
Asp718I GGTACC 1 cut(s) 691
AspLEI GCGC 3 cut(s) 579, 828, 979
AspS9I GGNCC 3 cut(s) 413, 436, 778
AsuC2I CCSGG 1 cut(s) 429
AsuHPI GGTGA 2 cut(s) 883, 1028
AsuNHI GCTAGC 1 cut(s) 622
AvaII GGWCC 2 cut(s) 413, 778
AxyI CCTNAGG 1 cut(s) 1036
BanI GGYRCC 1 cut(s) 691
BanII GRGCYC 1 cut(s) 337
BbsI GAAGAC 3 cut(s) 440, 1004, 1023
Bbv12I GWGCWC 2 cut(s) 38, 337
BbvI GCAGC 3 cut(s) 226, 591, 758
BccI CCATC 2 cut(s) 412, 570
BclI TGATCA 2 cut(s) 187, 1018
BcnI CCSGG 1 cut(s) 429
BcoDI GTCTC 4 cut(s) 398, 532, 674, 1045
BfaI CTAG 1 cut(s) 623
BfoI RGCGCY 1 cut(s) 980
BisI GCNGC 5 cut(s) 215, 507, 580, 772, 941
BlsI GCNGC 5 cut(s) 216, 508, 581, 773, 942
BmcAI AGTACT 1 cut(s) 953
Bme1390I CCNGG 1 cut(s) 429
Bme18I GGWCC 2 cut(s) 413, 778
BmgBI CACGTC 1 cut(s) 869
BmgT120I GGNCC 3 cut(s) 413, 436, 778
BmiI GGNNCC 2 cut(s) 438, 693
BmrFI CCNGG 1 cut(s) 429
BmsI GCATC 4 cut(s) 96, 120, 144, 952
BmtI GCTAGC 1 cut(s) 626
BpiI GAAGAC 3 cut(s) 440, 1004, 1023
BpmI CTGGAG 1 cut(s) 329
BpuEI CTTGAG 2 cut(s) 850, 892
BpuMI CCSGG 1 cut(s) 429
BsaBI GATNNNNATC 1 cut(s) 1023
BsaHI GRCGYC 1 cut(s) 492
BsaJI CCNNGG 2 cut(s) 218, 495
BsaWI WCCGGW 2 cut(s) 688, 893
Bse118I RCCGGY 1 cut(s) 1004
Bse1I ACTGG 1 cut(s) 346
Bse21I CCTNAGG 1 cut(s) 1036
Bse3DI GCAATG 1 cut(s) 617
Bse8I GATNNNNATC 1 cut(s) 1023
BseDI CCNNGG 2 cut(s) 218, 495
BseGI GGATG 1 cut(s) 305
BseJI GATNNNNATC 1 cut(s) 1023
BseMI GCAATG 1 cut(s) 617
BseMII CTCAG 5 cut(s) 53, 403, 522, 837, 1050
BseNI ACTGG 1 cut(s) 346
BseRI GAGGAG 5 cut(s) 189, 192, 263, 273, 550
BseXI GCAGC 3 cut(s) 226, 591, 758
BseYI CCCAGC 1 cut(s) 590
Bsh1285I CGRYCG 2 cut(s) 699, 785
BshFI GGCC 3 cut(s) 437, 455, 761
BshNI GGYRCC 1 cut(s) 691
BsiEI CGRYCG 2 cut(s) 699, 785
BsiHKAI GWGCWC 2 cut(s) 38, 337
BsiSI CCGG 4 cut(s) 429, 689, 894, 1005
BsmAI GTCTC 4 cut(s) 398, 532, 674, 1045
BsmBI CGTCTC 3 cut(s) 398, 532, 674
BsnI GGCC 3 cut(s) 437, 455, 761
Bsp1286I GDGCHC 2 cut(s) 38, 337
Bsp143I GATC 8 cut(s) 22, 187, 672, 782, 787, 849, 1018, 1024
Bsp19I CCATGG 1 cut(s) 218
BspANI GGCC 3 cut(s) 437, 455, 761
BspCNI CTCAG 5 cut(s) 52, 402, 523, 838, 1049
BspLI GGNNCC 2 cut(s) 438, 693
BspOI GCTAGC 1 cut(s) 626
BspPI GGATC 2 cut(s) 795, 1032
BspQI GCTCTTC 1 cut(s) 325
BspT107I GGYRCC 1 cut(s) 691
BsrDI GCAATG 1 cut(s) 617
BsrFI RCCGGY 1 cut(s) 1004
BsrI ACTGG 1 cut(s) 346
BssAI RCCGGY 1 cut(s) 1004
BssECI CCNNGG 2 cut(s) 218, 495
BssMI GATC 8 cut(s) 22, 187, 672, 782, 787, 849, 1018, 1024
BssNI GRCGYC 1 cut(s) 492
BssT1I CCWWGG 2 cut(s) 218, 495
Bst4CI ACNGT 2 cut(s) 362, 743
Bst6I CTCTTC 3 cut(s) 325, 538, 894
BstACI GRCGYC 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 624, 1006
BstDEI CTNAG 7 cut(s) 39, 389, 513, 531, 846, 1036, 1047
BstDSI CCRYGG 1 cut(s) 218
BstF5I GGATG 1 cut(s) 305
BstH2I RGCGCY 1 cut(s) 980
BstHHI GCGC 3 cut(s) 579, 828, 979
BstKTI GATC 8 cut(s) 25, 190, 675, 785, 790, 852, 1021, 1027
BstMAI GTCTC 4 cut(s) 398, 532, 674, 1045
BstMBI GATC 8 cut(s) 22, 187, 672, 782, 787, 849, 1018, 1024
BstMCI CGRYCG 2 cut(s) 699, 785
BstMWI GCNNNNNNNGC 3 cut(s) 506, 946, 983
BstNSI RCATGY 1 cut(s) 644
BstSCI CCNGG 1 cut(s) 427
BstV1I GCAGC 3 cut(s) 226, 591, 758
BstV2I GAAGAC 3 cut(s) 440, 1004, 1023
BstX2I RGATCY 1 cut(s) 787
BstYI RGATCY 1 cut(s) 787
Bsu36I CCTNAGG 1 cut(s) 1036
BsuRI GGCC 3 cut(s) 437, 455, 761
BtgI CCRYGG 1 cut(s) 218
BtrI CACGTC 1 cut(s) 869
BtsCI GGATG 1 cut(s) 305
Cac8I GCNNGC 2 cut(s) 624, 1006
CfoI GCGC 3 cut(s) 579, 828, 979
Cfr10I RCCGGY 1 cut(s) 1004
Cfr13I GGNCC 3 cut(s) 413, 436, 778
CseI GACGC 2 cut(s) 415, 500
Csp6I GTAC 2 cut(s) 692, 952
CviAII CATG 5 cut(s) 185, 219, 487, 641, 991
CviQI GTAC 2 cut(s) 692, 952
DdeI CTNAG 7 cut(s) 39, 389, 513, 531, 846, 1036, 1047
DpnI GATC 8 cut(s) 24, 189, 674, 784, 789, 851, 1020, 1026
DpnII GATC 8 cut(s) 22, 187, 672, 782, 787, 849, 1018, 1024
Eam1104I CTCTTC 3 cut(s) 325, 538, 894
EarI CTCTTC 3 cut(s) 325, 538, 894
EciI GGCGGA 1 cut(s) 879
Ecl136II GAGCTC 1 cut(s) 335
Eco130I CCWWGG 2 cut(s) 218, 495
Eco147I AGGCCT 1 cut(s) 761
Eco24I GRGCYC 1 cut(s) 337
Eco47I GGWCC 2 cut(s) 413, 778
Eco47III AGCGCT 1 cut(s) 978
Eco53kI GAGCTC 1 cut(s) 335
Eco57I CTGAAG 2 cut(s) 434, 1001
Eco81I CCTNAGG 1 cut(s) 1036
EcoICRI GAGCTC 1 cut(s) 335
EcoT14I CCWWGG 2 cut(s) 218, 495
EcoT38I GRGCYC 1 cut(s) 337
ErhI CCWWGG 2 cut(s) 218, 495
Esp3I CGTCTC 3 cut(s) 398, 532, 674
FaeI CATG 5 cut(s) 188, 222, 490, 644, 994
FaiI YATR 7 cut(s) 186, 220, 312, 355, 488, 642, 992
FalI AAGNNNNNCTT 2 cut(s) 322, 354
FatI CATG 5 cut(s) 184, 218, 486, 640, 990
FbaI TGATCA 2 cut(s) 187, 1018
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 5 cut(s) 215, 507, 580, 772, 941
FokI GGATG 1 cut(s) 292
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 5 cut(s) 215, 507, 580, 772, 941
FspBI CTAG 1 cut(s) 623
FspI TGCGCA 1 cut(s) 578
GlaI GCGC 3 cut(s) 578, 827, 978
GluI GCNGC 5 cut(s) 215, 507, 580, 772, 941
GsaI CCCAGC 1 cut(s) 594
GsuI CTGGAG 1 cut(s) 329
HaeII RGCGCY 1 cut(s) 980
HaeIII GGCC 3 cut(s) 437, 455, 761
HapII CCGG 4 cut(s) 429, 689, 894, 1005
HgaI GACGC 2 cut(s) 415, 500
HhaI GCGC 3 cut(s) 579, 828, 979
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 5 cut(s) 188, 222, 490, 644, 994
Hin6I GCGC 3 cut(s) 577, 826, 977
HinP1I GCGC 3 cut(s) 577, 826, 977
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HindIII AAGCTT 2 cut(s) 60, 612
HinfI GANTC 6 cut(s) 223, 517, 552, 685, 728, 905
HpaII CCGG 4 cut(s) 429, 689, 894, 1005
HphI GGTGA 2 cut(s) 883, 1028
Hpy166II GTNNAC 3 cut(s) 351, 471, 721
Hpy188I TCNGA 9 cut(s) 105, 129, 153, 373, 392, 403, 672, 733, 787
Hpy188III TCNNGA 2 cut(s) 808, 1022
Hpy8I GTNNAC 3 cut(s) 351, 471, 721
Hpy99I CGWCG 2 cut(s) 301, 362
HpyAV CCTTC 3 cut(s) 253, 348, 976
HpyCH4III ACNGT 2 cut(s) 362, 743
HpyCH4IV ACGT 2 cut(s) 868, 879
HpyCH4V TGCA 5 cut(s) 109, 133, 157, 214, 957
HpyF10VI GCNNNNNNNGC 3 cut(s) 506, 946, 983
HpyF3I CTNAG 7 cut(s) 39, 389, 513, 531, 846, 1036, 1047
HpySE526I ACGT 2 cut(s) 868, 879
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 5 cut(s) 188, 222, 490, 644, 994
HspAI GCGC 3 cut(s) 577, 826, 977
KpnI GGTACC 1 cut(s) 695
KroI GCCGGC 1 cut(s) 1004
KroNI GCCGGC 1 cut(s) 1006
Ksp22I TGATCA 2 cut(s) 187, 1018
Kzo9I GATC 8 cut(s) 22, 187, 672, 782, 787, 849, 1018, 1024
LguI GCTCTTC 1 cut(s) 325
LmnI GCTCC 2 cut(s) 340, 925
Lsp1109I GCAGC 3 cut(s) 226, 591, 758
LweI GCATC 4 cut(s) 96, 120, 144, 952
MaeI CTAG 1 cut(s) 623
MaeII ACGT 2 cut(s) 868, 879
MaeIII GTNAC 1 cut(s) 518
MalI GATC 8 cut(s) 24, 189, 674, 784, 789, 851, 1020, 1026
MboI GATC 8 cut(s) 22, 187, 672, 782, 787, 849, 1018, 1024
MflI RGATCY 1 cut(s) 787
MhlI GDGCHC 2 cut(s) 38, 337
MluCI AATT 2 cut(s) 291, 393
MlyI GAGTC 4 cut(s) 217, 526, 694, 899
MmeI TCCRAC 1 cut(s) 109
MreI CGCCGGCG 1 cut(s) 1004
MroNI GCCGGC 1 cut(s) 1004
MspI CCGG 4 cut(s) 429, 689, 894, 1005
MspR9I CCNGG 1 cut(s) 429
MwoI GCNNNNNNNGC 3 cut(s) 506, 946, 983
NaeI GCCGGC 1 cut(s) 1006
NciI CCSGG 1 cut(s) 429
NcoI CCATGG 1 cut(s) 218
NdeII GATC 8 cut(s) 22, 187, 672, 782, 787, 849, 1018, 1024
NgoMIV GCCGGC 1 cut(s) 1004
NheI GCTAGC 1 cut(s) 622
NlaIII CATG 5 cut(s) 188, 222, 490, 644, 994
NlaIV GGNNCC 2 cut(s) 438, 693
NmeAIII GCCGAG 3 cut(s) 481, 703, 840
NmuCI GTSAC 1 cut(s) 518
NsbI TGCGCA 1 cut(s) 578
NspI RCATGY 1 cut(s) 644
PceI AGGCCT 1 cut(s) 761
PciI ACATGT 1 cut(s) 640
PciSI GCTCTTC 1 cut(s) 325
PcsI WCGNNNNNNNCGW 1 cut(s) 413
PdiI GCCGGC 1 cut(s) 1006
PfeI GAWTC 2 cut(s) 552, 728
PkrI GCNGC 5 cut(s) 216, 508, 581, 773, 942
Ple19I CGATCG 1 cut(s) 785
PleI GAGTC 4 cut(s) 217, 525, 693, 899
PpsI GAGTC 4 cut(s) 217, 525, 693, 899
PscI ACATGT 1 cut(s) 640
Psp124BI GAGCTC 1 cut(s) 337
PspFI CCCAGC 1 cut(s) 590
PspN4I GGNNCC 2 cut(s) 438, 693
PspPI GGNCC 3 cut(s) 413, 436, 778
PsuI RGATCY 1 cut(s) 787
PvuI CGATCG 1 cut(s) 785
RsaI GTAC 2 cut(s) 693, 953
RsaNI GTAC 2 cut(s) 692, 952
SacI GAGCTC 1 cut(s) 337
SapI GCTCTTC 1 cut(s) 325
SatI GCNGC 5 cut(s) 215, 507, 580, 772, 941
Sau3AI GATC 8 cut(s) 22, 187, 672, 782, 787, 849, 1018, 1024
Sau96I GGNCC 3 cut(s) 413, 436, 778
ScaI AGTACT 1 cut(s) 953
SchI GAGTC 4 cut(s) 217, 526, 694, 899
ScrFI CCNGG 1 cut(s) 429
SduI GDGCHC 2 cut(s) 38, 337
SfaNI GCATC 4 cut(s) 96, 120, 144, 952
SgrAI CRCCGGYG 1 cut(s) 1004
SinI GGWCC 2 cut(s) 413, 778
SmlI CTYRAG 2 cut(s) 829, 907
SmoI CTYRAG 2 cut(s) 829, 907
Sse9I AATT 2 cut(s) 291, 393
SseBI AGGCCT 1 cut(s) 761
SspMI CTAG 1 cut(s) 623
SstI GAGCTC 1 cut(s) 337
StuI AGGCCT 1 cut(s) 761
StyD4I CCNGG 1 cut(s) 427
StyI CCWWGG 2 cut(s) 218, 495
TaaI ACNGT 2 cut(s) 362, 743
TaiI ACGT 2 cut(s) 871, 882
TaqI TCGA 1 cut(s) 965
TaqII GACCGA 4 cut(s) 430, 434, 713, 795
TasI AATT 2 cut(s) 291, 393
TatI WGTACW 1 cut(s) 951
TauI GCSGC 2 cut(s) 509, 943
TfiI GAWTC 2 cut(s) 552, 728
TseFI GTSAC 1 cut(s) 518
TseI GCWGC 3 cut(s) 214, 579, 771
Tsp45I GTSAC 1 cut(s) 518
TspDTI ATGAA 2 cut(s) 606, 741
TspGWI ACGGA 2 cut(s) 285, 620
VpaK11BI GGWCC 2 cut(s) 413, 778
XapI RAATTY 1 cut(s) 393
XceI RCATGY 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 720
XspI CTAG 1 cut(s) 623
ZrmI AGTACT 1 cut(s) 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.