MD13G1054600.v1.1

WPP domain-interacting tail-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003026
Physical Location & Seq
Reverse (-)
3828562 .. 3832664
4103 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1054600.v1.1.491

Sequence Viewer

Length: 2037 bp
ATGGAAGACCACGCTGTCTGTGATATCAATGTTGTGCCATCTAATGAGTCTGGCGTGCAAGAGTTAGAGAGTTCCATGAAAGTTTTAACAAAGGTGGACTTGGACTTGGCATATTCTTCTGAGAAGTTAGCAAATCTCCATGTACCTCTGGTGTACCTTTTGGCTCAGCAAAGTGATCTCGAGGCAATGGCCACCGCGAATAACTATATTCTGGCAGACTTTATTGAAAAAGTATTGGCATTCAATCTCCTGTCTAGCATTTTAGATTCTGAGGTAAGAGAGCTGGACAGTTACATGGATAATCTCCGAGTGGATATTGTTGATGCGCGTCAGAAAATATCTTCGTGCAGACACTTGGGAGAACTGTACTCTATAATGAAAGGAAAACTAAATGATTCTGAAGAATCACTAAAGCAATCTCGACAGCAGATTTCAGAGGTCAAGATGCAGTCAGAAAGGTTTCAGAGAACTGTTCTGGCCTTCGCGCATGAGAATTGGAAAACTGACATTGCCATGGATTTATCTGAGAATGCACAACTGTCAAATAAAAATGCAATGTCAAATTTAAGGATGCCTAGACAACAAAGACATAATCTATGGATGCTGGAGAAGTCTCTTACAAGGGAGCTATATCTTGAGAAGAAATTAGCTGAGTCAAGACAAAATGAAGAAGAACTTAAAGTGAAGCTACATCATACTGAACAGGTATCATTTCGCATGGAAGAAGCTGCAGAAGTTGTTTGGGGAAGATTTTTAGAGGCAGAGAATGGTGCTGAGGTTCTCCAGGGAATTTCGAAGGAGTTATTAGGTAGACTGCAGCTTGTTCATTTTAATCTGAACGGTTCAATTCAACGAGAAAGTGAGTTAAAATCCCAACTTCAAGCTAGTCTGGAAGAGCTTAGAGCGAAAGATACTGCTTTGCTGAAGCTCCACAATATCCTTGCCGAGAACATTAAGAAAGATGTTGAACTGTCTGCTTTGAGAGAAAAGGTGATGCTCCTCGAGAAACAGCTGAAAGAATCACAGCGCCAGTTGAAGAATGTAAATGCCACGAATGAATCAAGTGAAGAGAAACTTGGGGAAATGGAAAGTTTAGTTGCCTCTTTGAAAGAAAGCATCTATGTAGCAGAAGTTAAGGCTGAGAATGCAGAACTTAAGGTCGCAGAATTAACAGGAACAAATATGGAACTCAACGAAGAGATGAGTTTTCTCAAAGGAAGCGCTACTGGCACAGAAAAAAAGGTTGGAAGTCTCGAGAAGCAGCTGAGGGAAGTAGAGATACAATTACAGCATGCCAAAGCATCCTCCGAAGCAAGTCAAGAGCAGCAGAATATGTTATATGCTGCAATTTGGGATATGGAAACTTTAATTGAAGATCTGAAATCAAAGGTTTCCAAAGCGGATATTAAGATAGAAAGTACCGAGGAGCAATGTATAATGTTATCTGAAACTAATGCAGAACTAAATGCGGAAATAACTGTTCTAAGGGATAGAGTGGAATACTTGGAATCGTGTTTGGATCAAGCTAACAATGCAAAACTTGCAAGTGCAGAAGAACTCAATTCCAGGATCAAGCTCATTATGGATATGGCAATGCAATTGGCCGTAGAAAGGGAGCGCATCCAAAATCAGATGCATGTCTTAACAATGGAGAAAAAACTTCTGGTGGAGAAGATATGGAACACCAAAAAAGATGCAACTGAGTGCAACAATAAAGACATTGACAACAAAGAGCTTCCGTCTTTCAAGTGTAATCCTCCAAGTGCTACTTCTATAAAAACACCCGTGGAAGATGTGACATATTCTTTAGATAAAAGTTTCCAGGTGGATGAAGCGTCAGCAGAGGCACCTGACTGTGATAATGATGCGGGGGCTTCCAGTTCTGCAAATAGTCCCACTGGCTCGGATACAGAACTTGACAATGTCGAAGTGGTAGAGGCCAAACGCCTGAGCCTCAAGTCTGTTTTCATGGTAATTGTTCTCATATCAGCCATATATTTGCTTAACCAGGAACCTTTCCTCGTTGACGAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008360 GO:0022603 GO:0022604 GO:0032878 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0065007 GO:0065008 GO:2000769
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

679

Amino Acids

76.64

Weight (kDa)

4.83

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIT1_2_N PF26581 22 - 161 1e-55 WIT1/2, N-terminal helical bundle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
AccB1I GGYRCC 1 cut(s) 1846
AccI GTMKAC 1 cut(s) 811
AccII CGCG 3 cut(s) 197, 328, 485
AciI CCGC 4 cut(s) 195, 1400, 1469, 1868
AclWI GGATC 2 cut(s) 1527, 1577
AcoI YGGCCR 2 cut(s) 189, 1602
AcsI RAATTY 2 cut(s) 562, 789
AcuI CTGAAG 2 cut(s) 420, 944
AfaI GTAC 4 cut(s) 144, 155, 368, 1420
AfeI AGCGCT 1 cut(s) 1222
AfiI CCNNNNNNNGG 1 cut(s) 1611
AflII CTTAAG 1 cut(s) 1154
AjnI CCWGG 4 cut(s) 783, 1565, 1821, 2007
AjuI GAANNNNNNNTTGG 6 cut(s) 1059, 1091, 1227, 1259, 1753, 1785
Alw26I GTCTC 2 cut(s) 618, 1256
AlwI GGATC 2 cut(s) 1527, 1577
Ama87I CYCGRG 3 cut(s) 179, 1001, 1253
Aor51HI AGCGCT 1 cut(s) 1222
AoxI GGCC 4 cut(s) 189, 477, 1602, 1938
ApeKI GCWGC 5 cut(s) 728, 817, 1261, 1324, 1343
ApoI RAATTY 2 cut(s) 562, 789
Asp700I GAANNNNTTC 1 cut(s) 459
AspLEI GCGC 5 cut(s) 328, 487, 1029, 1223, 1620
AsuHPI GGTGA 1 cut(s) 1003
AsuII TTCGAA 1 cut(s) 794
AvaI CYCGRG 3 cut(s) 179, 1001, 1253
BalI TGGCCA 1 cut(s) 191
BanI GGYRCC 1 cut(s) 1846
BarI GAAGNNNNNNTAC 2 cut(s) 1263, 1295
BbsI GAAGAC 1 cut(s) 12
BbvCI CCTCAGC 2 cut(s) 774, 1265
BbvI GCAGC 5 cut(s) 715, 829, 1273, 1330, 1336
BccI CCATC 1 cut(s) 46
BceAI ACGGC 1 cut(s) 1589
BciT130I CCWGG 4 cut(s) 785, 1567, 1823, 2009
BciVI GTATCC 1 cut(s) 1900
BcoDI GTCTC 2 cut(s) 618, 1256
BfaI CTAG 3 cut(s) 255, 576, 885
BfmI CTRYAG 2 cut(s) 729, 815
BfoI RGCGCY 2 cut(s) 1030, 1224
BfrI CTTAAG 1 cut(s) 1154
BfuI GTATCC 1 cut(s) 1900
BglII AGATCT 1 cut(s) 1375
BisI GCNGC 5 cut(s) 729, 818, 1262, 1325, 1344
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 5 cut(s) 730, 819, 1263, 1326, 1345
Bme1390I CCNGG 4 cut(s) 785, 1567, 1823, 2009
BmeT110I CYCGRG 3 cut(s) 179, 1001, 1253
BmiI GGNNCC 2 cut(s) 1848, 2013
BmrFI CCNGG 4 cut(s) 785, 1567, 1823, 2009
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 2 cut(s) 626, 767
Bpu10I CCTNAGC 3 cut(s) 774, 1265, 1949
Bpu1102I GCTNAGC 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 794
BpuEI CTTGAG 2 cut(s) 656, 1940
BsaJI CCNNGG 4 cut(s) 513, 784, 1422, 1785
Bsc4I CCNNNNNNNGG 1 cut(s) 1611
Bse1I ACTGG 4 cut(s) 1030, 1231, 1878, 1903
Bse3DI GCAATG 5 cut(s) 192, 507, 561, 1436, 1599
BseBI CCWGG 4 cut(s) 785, 1567, 1823, 2009
BseDI CCNNGG 4 cut(s) 513, 784, 1422, 1785
BseGI GGATG 5 cut(s) 576, 606, 1301, 1620, 1834
BseLI CCNNNNNNNGG 1 cut(s) 1611
BseMI GCAATG 5 cut(s) 192, 507, 561, 1436, 1599
BseNI ACTGG 4 cut(s) 1030, 1231, 1878, 1903
BseRI GAGGAG 2 cut(s) 989, 1439
BseXI GCAGC 5 cut(s) 715, 829, 1273, 1330, 1336
BsgI GTGCAG 2 cut(s) 367, 1569
Bsh1236I CGCG 3 cut(s) 197, 328, 485
BshFI GGCC 4 cut(s) 191, 479, 1604, 1940
BshNI GGYRCC 1 cut(s) 1846
BsiHKCI CYCGRG 3 cut(s) 179, 1001, 1253
BslFI GGGAC 1 cut(s) 1878
BslI CCNNNNNNNGG 1 cut(s) 1611
BsmAI GTCTC 2 cut(s) 618, 1256
BsmFI GGGAC 1 cut(s) 1878
BsmI GAATGC 3 cut(s) 239, 535, 1150
BsnI GGCC 4 cut(s) 191, 479, 1604, 1940
BsoBI CYCGRG 3 cut(s) 179, 1001, 1253
Bsp119I TTCGAA 1 cut(s) 794
Bsp143I GATC 4 cut(s) 175, 1375, 1519, 1569
Bsp1720I GCTNAGC 1 cut(s) 165
Bsp19I CCATGG 1 cut(s) 513
BspACI CCGC 4 cut(s) 195, 1400, 1469, 1868
BspANI GGCC 4 cut(s) 191, 479, 1604, 1940
BspFNI CGCG 3 cut(s) 197, 328, 485
BspLI GGNNCC 2 cut(s) 1848, 2013
BspMAI CTGCAG 2 cut(s) 733, 819
BspPI GGATC 2 cut(s) 1527, 1577
BspQI GCTCTTC 1 cut(s) 888
BspT104I TTCGAA 1 cut(s) 794
BspT107I GGYRCC 1 cut(s) 1846
BspTI CTTAAG 1 cut(s) 1154
BsrDI GCAATG 5 cut(s) 192, 507, 561, 1436, 1599
BsrI ACTGG 4 cut(s) 1030, 1231, 1878, 1903
BssECI CCNNGG 4 cut(s) 513, 784, 1422, 1785
BssMI GATC 4 cut(s) 175, 1375, 1519, 1569
BssT1I CCWWGG 1 cut(s) 513
Bst2UI CCWGG 4 cut(s) 785, 1567, 1823, 2009
Bst4CI ACNGT 8 cut(s) 290, 366, 472, 540, 842, 972, 1480, 1856
Bst6I CTCTTC 3 cut(s) 888, 1062, 1191
BstAFI CTTAAG 1 cut(s) 1154
BstAPI GCANNNNNTGC 1 cut(s) 1541
BstBI TTCGAA 1 cut(s) 794
BstC8I GCNNGC 2 cut(s) 56, 1293
BstDSI CCRYGG 2 cut(s) 513, 1785
BstF5I GGATG 5 cut(s) 576, 606, 1301, 1620, 1834
BstFNI CGCG 3 cut(s) 197, 328, 485
BstH2I RGCGCY 2 cut(s) 1030, 1224
BstHHI GCGC 5 cut(s) 328, 487, 1029, 1223, 1620
BstKTI GATC 4 cut(s) 178, 1378, 1522, 1572
BstMAI GTCTC 2 cut(s) 618, 1256
BstMBI GATC 4 cut(s) 175, 1375, 1519, 1569
BstMWI GCNNNNNNNGC 4 cut(s) 1145, 1227, 1532, 1541
BstNI CCWGG 4 cut(s) 785, 1567, 1823, 2009
BstNSI RCATGY 2 cut(s) 1295, 1640
BstSCI CCNGG 4 cut(s) 783, 1565, 1821, 2007
BstSFI CTRYAG 2 cut(s) 729, 815
BstUI CGCG 3 cut(s) 197, 328, 485
BstV1I GCAGC 5 cut(s) 715, 829, 1273, 1330, 1336
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 1375
BstYI RGATCY 1 cut(s) 1375
BsuI GTATCC 1 cut(s) 1900
BsuRI GGCC 4 cut(s) 191, 479, 1604, 1940
BtgI CCRYGG 2 cut(s) 513, 1785
BtsCI GGATG 5 cut(s) 576, 606, 1301, 1620, 1834
BtsIMutI CAGTG 1 cut(s) 1896
Cac8I GCNNGC 2 cut(s) 56, 1293
CfoI GCGC 5 cut(s) 328, 487, 1029, 1223, 1620
CseI GACGC 2 cut(s) 317, 1824
Csp6I GTAC 4 cut(s) 143, 154, 367, 1419
CviAII CATG 9 cut(s) 76, 140, 295, 488, 514, 718, 1292, 1637, 1969
CviQI GTAC 4 cut(s) 143, 154, 367, 1419
DpnI GATC 4 cut(s) 177, 1377, 1521, 1571
DpnII GATC 4 cut(s) 175, 1375, 1519, 1569
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
EaeI YGGCCR 2 cut(s) 189, 1602
Eam1104I CTCTTC 3 cut(s) 888, 1062, 1191
EarI CTCTTC 3 cut(s) 888, 1062, 1191
Eco130I CCWWGG 1 cut(s) 513
Eco32I GATATC 1 cut(s) 25
Eco47III AGCGCT 1 cut(s) 1222
Eco57I CTGAAG 2 cut(s) 420, 944
Eco88I CYCGRG 3 cut(s) 179, 1001, 1253
EcoRII CCWGG 4 cut(s) 783, 1565, 1821, 2007
EcoRV GATATC 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 513
EcoT22I ATGCAT 1 cut(s) 1638
ErhI CCWWGG 1 cut(s) 513
FaeI CATG 9 cut(s) 79, 143, 298, 491, 517, 721, 1295, 1640, 1972
FalI AAGNNNNNCTT 8 cut(s) 83, 115, 660, 692, 1059, 1091, 1753, 1785
FaqI GGGAC 1 cut(s) 1878
FatI CATG 9 cut(s) 75, 139, 294, 487, 513, 717, 1291, 1636, 1968
FauI CCCGC 1 cut(s) 1861
FblI GTMKAC 1 cut(s) 811
Fnu4HI GCNGC 5 cut(s) 729, 818, 1262, 1325, 1344
FokI GGATG 5 cut(s) 583, 613, 1288, 1607, 1841
Fsp4HI GCNGC 5 cut(s) 729, 818, 1262, 1325, 1344
FspBI CTAG 3 cut(s) 255, 576, 885
GlaI GCGC 5 cut(s) 327, 486, 1028, 1222, 1619
GluI GCNGC 5 cut(s) 729, 818, 1262, 1325, 1344
GsuI CTGGAG 2 cut(s) 626, 767
HaeII RGCGCY 2 cut(s) 1030, 1224
HaeIII GGCC 4 cut(s) 191, 479, 1604, 1940
HgaI GACGC 2 cut(s) 317, 1824
HhaI GCGC 5 cut(s) 328, 487, 1029, 1223, 1620
Hin1II CATG 9 cut(s) 79, 143, 298, 491, 517, 721, 1295, 1640, 1972
Hin6I GCGC 5 cut(s) 326, 485, 1027, 1221, 1618
HinP1I GCGC 5 cut(s) 326, 485, 1027, 1221, 1618
HincII GTYRAC 1 cut(s) 2026
HindII GTYRAC 1 cut(s) 2026
HinfI GANTC 8 cut(s) 47, 266, 395, 404, 653, 1019, 1058, 1508
HphI GGTGA 1 cut(s) 1003
Hpy166II GTNNAC 4 cut(s) 97, 154, 812, 2026
Hpy8I GTNNAC 4 cut(s) 97, 154, 812, 2026
HpyAV CCTTC 2 cut(s) 490, 790
HpyCH4III ACNGT 8 cut(s) 290, 366, 472, 540, 842, 972, 1480, 1856
HpyF10VI GCNNNNNNNGC 4 cut(s) 1145, 1227, 1532, 1541
Hsp92II CATG 9 cut(s) 79, 143, 298, 491, 517, 721, 1295, 1640, 1972
HspAI GCGC 5 cut(s) 326, 485, 1027, 1221, 1618
Kzo9I GATC 4 cut(s) 175, 1375, 1519, 1569
LguI GCTCTTC 1 cut(s) 888
LmnI GCTCC 5 cut(s) 625, 933, 1002, 1426, 1615
Lsp1109I GCAGC 5 cut(s) 715, 829, 1273, 1330, 1336
MaeI CTAG 3 cut(s) 255, 576, 885
MaeIII GTNAC 2 cut(s) 290, 1795
MalI GATC 4 cut(s) 177, 1377, 1521, 1571
MboI GATC 4 cut(s) 175, 1375, 1519, 1569
MfeI CAATTG 1 cut(s) 1598
MflI RGATCY 1 cut(s) 1375
MlsI TGGCCA 1 cut(s) 191
MluNI TGGCCA 1 cut(s) 191
MlyI GAGTC 2 cut(s) 56, 662
MmeI TCCRAC 1 cut(s) 1225
Mox20I TGGCCA 1 cut(s) 191
Mph1103I ATGCAT 1 cut(s) 1638
MroXI GAANNNNTTC 1 cut(s) 459
MscI TGGCCA 1 cut(s) 191
MslI CAYNNNNRTG 1 cut(s) 512
Msp20I TGGCCA 1 cut(s) 191
MspA1I CMGCKG 2 cut(s) 1012, 1264
MspCI CTTAAG 1 cut(s) 1154
MspR9I CCNGG 4 cut(s) 785, 1567, 1823, 2009
MunI CAATTG 1 cut(s) 1598
Mva1269I GAATGC 3 cut(s) 239, 535, 1150
MvaI CCWGG 4 cut(s) 785, 1567, 1823, 2009
MvnI CGCG 3 cut(s) 197, 328, 485
MwoI GCNNNNNNNGC 4 cut(s) 1145, 1227, 1532, 1541
NcoI CCATGG 1 cut(s) 513
NdeII GATC 4 cut(s) 175, 1375, 1519, 1569
NlaIII CATG 9 cut(s) 79, 143, 298, 491, 517, 721, 1295, 1640, 1972
NlaIV GGNNCC 2 cut(s) 1848, 2013
NmeAIII GCCGAG 1 cut(s) 970
NmuCI GTSAC 1 cut(s) 1795
NsiI ATGCAT 1 cut(s) 1638
NspI RCATGY 2 cut(s) 1295, 1640
NspV TTCGAA 1 cut(s) 794
PaeI GCATGC 1 cut(s) 1295
PaeR7I CTCGAG 3 cut(s) 179, 1001, 1253
PciSI GCTCTTC 1 cut(s) 888
PctI GAATGC 3 cut(s) 239, 535, 1150
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 6 cut(s) 266, 395, 404, 1019, 1058, 1508
PflFI GACNNNGTC 1 cut(s) 1922
PfoI TCCNGGA 1 cut(s) 1565
PkrI GCNGC 5 cut(s) 730, 819, 1263, 1326, 1345
PleI GAGTC 2 cut(s) 55, 661
PpsI GAGTC 2 cut(s) 55, 661
Psp6I CCWGG 4 cut(s) 783, 1565, 1821, 2007
PspGI CCWGG 4 cut(s) 783, 1565, 1821, 2007
PspN4I GGNNCC 2 cut(s) 1848, 2013
PstI CTGCAG 2 cut(s) 733, 819
PsuI RGATCY 1 cut(s) 1375
PsyI GACNNNGTC 1 cut(s) 1922
PvuII CAGCTG 2 cut(s) 1012, 1264
RsaI GTAC 4 cut(s) 144, 155, 368, 1420
RsaNI GTAC 4 cut(s) 143, 154, 367, 1419
RseI CAYNNNNRTG 1 cut(s) 512
SapI GCTCTTC 1 cut(s) 888
SatI GCNGC 5 cut(s) 729, 818, 1262, 1325, 1344
Sau3AI GATC 4 cut(s) 175, 1375, 1519, 1569
SchI GAGTC 2 cut(s) 56, 662
ScrFI CCNGG 4 cut(s) 785, 1567, 1823, 2009
SfcI CTRYAG 2 cut(s) 729, 815
Sfr274I CTCGAG 3 cut(s) 179, 1001, 1253
SfuI TTCGAA 1 cut(s) 794
SlaI CTCGAG 3 cut(s) 179, 1001, 1253
SmiMI CAYNNNNRTG 1 cut(s) 512
SmlI CTYRAG 6 cut(s) 179, 635, 1001, 1154, 1253, 1955
SmoI CTYRAG 6 cut(s) 179, 635, 1001, 1154, 1253, 1955
SphI GCATGC 1 cut(s) 1295
SsiI CCGC 4 cut(s) 195, 1400, 1469, 1868
SspMI CTAG 3 cut(s) 255, 576, 885
StyD4I CCNGG 4 cut(s) 783, 1565, 1821, 2007
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 8 cut(s) 290, 366, 472, 540, 842, 972, 1480, 1856
TaqI TCGA 6 cut(s) 180, 421, 794, 1002, 1254, 1926
TatI WGTACW 1 cut(s) 366
TfiI GAWTC 6 cut(s) 266, 395, 404, 1019, 1058, 1508
TscAI CASTG 1 cut(s) 1903
TseFI GTSAC 1 cut(s) 1795
TseI GCWGC 5 cut(s) 728, 817, 1261, 1324, 1343
Tsp45I GTSAC 1 cut(s) 1795
TspDTI ATGAA 7 cut(s) 92, 392, 681, 815, 1071, 1845, 1957
TspGWI ACGGA 1 cut(s) 1728
TspRI CASTG 1 cut(s) 1903
Tth111I GACNNNGTC 1 cut(s) 1922
Vha464I CTTAAG 1 cut(s) 1154
XapI RAATTY 2 cut(s) 562, 789
XceI RCATGY 2 cut(s) 1295, 1640
XhoI CTCGAG 3 cut(s) 179, 1001, 1253
XmiI GTMKAC 1 cut(s) 811
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 3 cut(s) 255, 576, 885
Zsp2I ATGCAT 1 cut(s) 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.