Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0005807

Physical Location & Seq

Genomic Coordinates Reverse (-)

6833836 .. 6838261

Gene Structure 4426 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1096600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1947 bp

ATGTCCAATTTTATCTTACTTCTGCTCCTTCCTCTGTTTTTGTTTCTAAAGAGCGCTCGTTCTGACCCGAGAGCCACACAGGCAGCCTTGATATGCAGAAACGACACCATTCAGATCCCAGAACGTCAAACCTTCATCTCCAACTTCTTAGCAGCCATGAACGCCGTCACCCCACAGATTGCCACCCGAAGATACGCCAGCGTCGTCACCGGAACCGGAAACGTAACCGTTTTCGCCTTCGGCGAGTGCATGAAAGACGTCTCCAAAACGGACTGCGACCAGTGCTTCGCCCAGTGCAAAATCAAGATTTTAGGGTGCCTCCAATTTGTGAAAGCAACTCGCGGGGGCCGGCTTTTCTTCGACGGCTGCTACCTCCGGCACGATGATTTCGATTTCTTCAACCAGAGCTTGAGTTCCGAAGACAGGACCGTTTGTGGGGACAGAGATTTTGGTCGGAATCGGACAGTTTTTAAGGATAATGCTTTGAGGTTGGTGAGGAATTTGAGTTCGTTGGCGCCGAGGAATGATGGGTTTTCTGCTGGGTTTGTGGTGGGTGAAGGGAACGTTTCGGTTTACGGGTTGGCGCAGTGTTGGGAGTTTGTGAAGGGGCCTGGTTGCGAGGAGTGTTTGGCTGACGCCGTTAAGAGAATCGGATCTTGCACTCCGAAAGAAGAAGGGAGGGCTTTGAATGCTGGGTGTTATTTGAGGTATTCGACTCAGAAGTTTTATGATAACAATACAGTCACTGATGATGCAGGACGAAATGGAGGACGCCGCAGCTTGGCAATAATTTTGGCTGTAACATTTGCTGTTGTGCTGACTATTTCTGCGGTTGCTTTCTTTGCAAGGAAGAAAATACTGACGAGGAGGAAAGCTGCTTCGGAGAAAAAGCAATTCGGAGCTCTGTTTGCCACTGTGAATAAGTCCCAACTCAATTTTTCATACGAAGTTCTCGAGAAGGCCACCAATTACTTTCATGATGCCAACAAGCTTGGACAAGGAGGATCTGGCTCAGTGTATAAGGGAGTTCTGCCGGATGGAAATGTTGTTGCCATAAAGAGGCTGTTCTTCAACGCAAGGCAATGGGTGGATCATTTCTTCAACGAAGTTAATTTGATCAGCGGAATTCATCATAAAAATCTTTTAAAGCTGTTGGGGTGCAGCATTACAGGCCCCGAGAGCCTTCTTGTTTATGAGTATGTCCCAAATCAAAGCCTTCATGATTATTTTATTGTGAAAAACAATGTGGAGCCACTGAGGTGGGATTTGAGGTATAAAATCATACTGGGAACAGCTGAGGGATTGGCCTATCTTCACGAAGAATCGAAATTGAGGATCATTCATAGGGATATAAAACTGAGCAACATTCTGCTTGACGAGGAATTCAATGCAAAGATTGCTGATTTCGGACTTGCTAGAATGTTCCCTGAAGATAAAACTCACATAAGCACTGCCATTGCTGGCACACTAGGTTACATGGCTCCGGAGTATGTCGTTCGTGGCATGTTGACCGAGAAGGCAGATGTTTACAGTTTTGGAGTTCTTGTCATCGAAATCATCAGTGGAAGGAGGAACAATTCTGTCTTTTCAAGTTCGACTTCTATCCTGAAAATGACCTGGGATCTTTATGGAACTTGTAGGTTATGTGAAGCTGTCGATCCACTCCTAGAAGGTAAGTTTGAAAATGAAGAAGCATCTCGGTTACTTCAAATAGGGTTACTTTGTATGCAAGCTTCAGCAGAGCTGCGGCCGGCAATGTCATTAGTCGTGAAAATGCTTACCGAAAATCTTGAGATTCCTCAGCCAACACAACCCCCATTTCTCAATTCTGGTAGCGCAGTACTCAACAGGACAATTCCTTCAGGAAGTCTCATTTCACTGCCCGCGGAGTCTAACACGCAATCTTCGAAGAATAGCATGATCCAAAGCTGGATTGAGCCGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

649

Amino Acids

72.06

Weight (kDa)

8.69

Isoelectric Point (pI)

43.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	32 - 128	2.5e-09	Salt stress response/antifungal
Stress-antifung	PF01657	150 - 240	1.3e-13	Salt stress response/antifungal
Pkinase	PF00069	325 - 534	5.1e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	329 - 592	9.3e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005807)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70530 AT1G70530 AT1G70530
fragaria_vesca	FvH4_4g26070 FvH4_4g26070 FvH4_4g26070 FvH4_4g26070 FvH4_4g26070 FvH4_4g26070 FvH4_4g26070 FvH4_4g26070
malus_domestica	MD13G1096600.v1.1 MD16G1097600.v1.1
prunus_persica	Prupe.1G249300_v2.0.a1
pyrus_communis	pycom13g08420 pycom16g08300
rosa_chinensis	RchiOBHm_Chr4g0433521
rosa_laevigata	RLG00000006746
rosa_multiflora	Rmu_co8500781.1_g000001 Rmu_sc0000312.1_g000008
rosa_roxburghii	Rroxscaffold_5G00375040
rosa_rugosa	Rorug04G0269300
rosa_samantha	Rh4AG324000 Rh4BG332100 Rh4CG347400 Rh4DG328400
rosa_wichuraiana	Rw0G020160 Rw4G028070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	261
AccB1I	GGYRCC	2 cut(s)	315, 514
AccII	CGCG	2 cut(s)	342, 1886
AccIII	TCCGGA	1 cut(s)	1483
AciI	CCGC	7 cut(s)	342, 775, 830, 1122, 1747, 1884, 1886
AclI	AACGTT	1 cut(s)	564
AclWI	GGATC	8 cut(s)	109, 661, 1012, 1098, 1343, 1629, 1652, 1915
AcoI	YGGCCR	1 cut(s)	1748
AcsI	RAATTY	3 cut(s)	499, 1125, 1382
AcuI	CTGAAG	3 cut(s)	1449, 1719, 1845
AcyI	GRCGYC	4 cut(s)	258, 515, 636, 772
AfaI	GTAC	1 cut(s)	1842
AfeI	AGCGCT	1 cut(s)	55
AfiI	CCNNNNNNNGG	4 cut(s)	423, 435, 781, 1059
AgsI	TTSAA	8 cut(s)	400, 688, 1072, 1102, 1387, 1590, 1682, 1709
AjnI	CCWGG	2 cut(s)	610, 1616
AleI	CACNNNNGTG	1 cut(s)	1258
AloI	GAACNNNNNNTCC	2 cut(s)	490, 522
Alw21I	GWGCWC	1 cut(s)	904
Alw26I	GTCTC	2 cut(s)	265, 1874
AlwI	GGATC	8 cut(s)	109, 661, 1012, 1098, 1343, 1629, 1652, 1915
AlwNI	CAGNNNCTG	1 cut(s)	746
Ama87I	CYCGRG	3 cut(s)	67, 953, 1175
Aor13HI	TCCGGA	1 cut(s)	1483
Aor51HI	AGCGCT	1 cut(s)	55
AoxI	GGCC	6 cut(s)	346, 608, 960, 1171, 1305, 1748
ApeKI	GCWGC	7 cut(s)	83, 152, 366, 777, 875, 1161, 1744
ApoI	RAATTY	3 cut(s)	499, 1125, 1382
ArsI	GACNNNNNNTTYG	4 cut(s)	269, 301, 431, 463
AspLEI	GCGC	4 cut(s)	56, 517, 586, 1838
AspS9I	GGNCC	4 cut(s)	346, 426, 608, 1172
AsuHPI	GGTGA	4 cut(s)	160, 199, 505, 566
AsuII	TTCGAA	1 cut(s)	1907
AvaI	CYCGRG	3 cut(s)	67, 953, 1175
AvaII	GGWCC	1 cut(s)	426
BanI	GGYRCC	2 cut(s)	315, 514
BanII	GRGCYC	1 cut(s)	904
BarI	GAAGNNNNNNTAC	2 cut(s)	1717, 1749
BbsI	GAAGAC	1 cut(s)	426
Bbv12I	GWGCWC	1 cut(s)	904
BbvCI	CCTCAGC	2 cut(s)	1296, 1800
BbvI	GCAGC	7 cut(s)	95, 164, 353, 789, 862, 1173, 1731
BccI	CCATC	2 cut(s)	521, 1031
BceAI	ACGGC	3 cut(s)	149, 379, 623
BciT130I	CCWGG	2 cut(s)	612, 1618
BclI	TGATCA	1 cut(s)	1116
BcoDI	GTCTC	2 cut(s)	265, 1874
BfaI	CTAG	3 cut(s)	1416, 1469, 1667
BfoI	RGCGCY	2 cut(s)	57, 518
BglI	GCCNNNNNGGC	1 cut(s)	80
BisI	GCNGC	9 cut(s)	84, 153, 367, 775, 778, 876, 1162, 1745, 1748
BlsI	GCNGC	9 cut(s)	85, 154, 368, 776, 779, 877, 1163, 1746, 1749
BmcAI	AGTACT	1 cut(s)	1842
Bme1390I	CCNGG	2 cut(s)	612, 1618
Bme18I	GGWCC	1 cut(s)	426
BmeT110I	CYCGRG	3 cut(s)	67, 953, 1175
BmgT120I	GGNCC	4 cut(s)	346, 426, 608, 1172
BmiI	GGNNCC	8 cut(s)	214, 317, 347, 516, 609, 1174, 1251, 1482
BmrFI	CCNGG	2 cut(s)	612, 1618
BmrI	ACTGGG	2 cut(s)	286, 1295
BmsI	GCATC	3 cut(s)	742, 970, 1703
BmuI	ACTGGG	2 cut(s)	286, 1295
BpiI	GAAGAC	1 cut(s)	426
Bpu10I	CCTNAGC	2 cut(s)	1296, 1800
Bpu14I	TTCGAA	1 cut(s)	1907
BpuEI	CTTGAG	2 cut(s)	430, 1811
BsaHI	GRCGYC	4 cut(s)	258, 515, 636, 772
BsaJI	CCNNGG	3 cut(s)	518, 1617, 1884
BsaWI	WCCGGW	3 cut(s)	209, 215, 1483
BsaXI	ACNNNNNCTCC	4 cut(s)	9, 39, 1530, 1560
Bsc4I	CCNNNNNNNGG	4 cut(s)	423, 435, 781, 1059
Bse118I	RCCGGY	2 cut(s)	348, 1750
Bse1I	ACTGG	3 cut(s)	280, 292, 1290
Bse3DI	GCAATG	3 cut(s)	1088, 1455, 1761
BseAI	TCCGGA	1 cut(s)	1483
BseBI	CCWGG	2 cut(s)	612, 1618
BseDI	CCNNGG	3 cut(s)	518, 1617, 1884
BseGI	GGATG	1 cut(s)	1042
BseLI	CCNNNNNNNGG	4 cut(s)	423, 435, 781, 1059
BseMI	GCAATG	3 cut(s)	1088, 1455, 1761
BseMII	CTCAG	6 cut(s)	731, 1026, 1247, 1287, 1349, 1814
BseNI	ACTGG	3 cut(s)	280, 292, 1290
BseRI	GAGGAG	2 cut(s)	635, 880
BseX3I	CGGCCG	1 cut(s)	1748
BseXI	GCAGC	7 cut(s)	95, 164, 353, 789, 862, 1173, 1731
BseYI	CCCAGC	2 cut(s)	539, 692
BsgI	GTGCAG	1 cut(s)	1180
Bsh1236I	CGCG	2 cut(s)	342, 1886
Bsh1285I	CGRYCG	1 cut(s)	1751
BshFI	GGCC	6 cut(s)	348, 610, 962, 1173, 1307, 1750
BshNI	GGYRCC	2 cut(s)	315, 514
BsiEI	CGRYCG	1 cut(s)	1751
BsiHKAI	GWGCWC	1 cut(s)	904
BsiHKCI	CYCGRG	3 cut(s)	67, 953, 1175
BsiSI	CCGG	7 cut(s)	210, 216, 349, 376, 1034, 1484, 1751
BslFI	GGGAC	3 cut(s)	452, 910, 1187
BslI	CCNNNNNNNGG	4 cut(s)	423, 435, 781, 1059
BsmAI	GTCTC	2 cut(s)	265, 1874
BsmBI	CGTCTC	1 cut(s)	265
BsmFI	GGGAC	3 cut(s)	452, 910, 1187
BsmI	GAATGC	1 cut(s)	694
BsnI	GGCC	6 cut(s)	348, 610, 962, 1173, 1307, 1750
BsoBI	CYCGRG	3 cut(s)	67, 953, 1175
Bsp119I	TTCGAA	1 cut(s)	1907
Bsp1286I	GDGCHC	1 cut(s)	904
Bsp13I	TCCGGA	1 cut(s)	1483
Bsp143I	GATC	9 cut(s)	114, 653, 1004, 1090, 1116, 1335, 1621, 1657, 1920
BspACI	CCGC	7 cut(s)	342, 775, 830, 1122, 1747, 1884, 1886
BspANI	GGCC	6 cut(s)	348, 610, 962, 1173, 1307, 1750
BspCNI	CTCAG	6 cut(s)	730, 1025, 1248, 1288, 1350, 1813
BspEI	TCCGGA	1 cut(s)	1483
BspFNI	CGCG	2 cut(s)	342, 1886
BspHI	TCATGA	2 cut(s)	976, 1219
BspLI	GGNNCC	8 cut(s)	214, 317, 347, 516, 609, 1174, 1251, 1482
BspPI	GGATC	8 cut(s)	109, 661, 1012, 1098, 1343, 1629, 1652, 1915
BspT104I	TTCGAA	1 cut(s)	1907
BspT107I	GGYRCC	2 cut(s)	315, 514
BsrDI	GCAATG	3 cut(s)	1088, 1455, 1761
BsrFI	RCCGGY	2 cut(s)	348, 1750
BsrI	ACTGG	3 cut(s)	280, 292, 1290
BssAI	RCCGGY	2 cut(s)	348, 1750
BssECI	CCNNGG	3 cut(s)	518, 1617, 1884
BssMI	GATC	9 cut(s)	114, 653, 1004, 1090, 1116, 1335, 1621, 1657, 1920
BssNI	GRCGYC	4 cut(s)	258, 515, 636, 772
Bst2UI	CCWGG	2 cut(s)	612, 1618
Bst4CI	ACNGT	6 cut(s)	229, 430, 466, 742, 916, 1532
BstACI	GRCGYC	4 cut(s)	258, 515, 636, 772
BstAPI	GCANNNNNTGC	1 cut(s)	1397
BstBI	TTCGAA	1 cut(s)	1907
BstC8I	GCNNGC	6 cut(s)	199, 350, 1462, 1731, 1752, 1884
BstDEI	CTNAG	7 cut(s)	148, 717, 1012, 1256, 1296, 1358, 1800
BstDSI	CCRYGG	1 cut(s)	1884
BstF5I	GGATG	1 cut(s)	1042
BstFNI	CGCG	2 cut(s)	342, 1886
BstH2I	RGCGCY	2 cut(s)	57, 518
BstHHI	GCGC	4 cut(s)	56, 517, 586, 1838
BstKTI	GATC	9 cut(s)	117, 656, 1007, 1093, 1119, 1338, 1624, 1660, 1923
BstMAI	GTCTC	2 cut(s)	265, 1874
BstMBI	GATC	9 cut(s)	114, 653, 1004, 1090, 1116, 1335, 1621, 1657, 1920
BstMCI	CGRYCG	1 cut(s)	1751
BstMWI	GCNNNNNNNGC	9 cut(s)	80, 161, 282, 689, 842, 908, 1170, 1179, 1397
BstNI	CCWGG	2 cut(s)	612, 1618
BstNSI	RCATGY	1 cut(s)	1507
BstSCI	CCNGG	2 cut(s)	610, 1616
BstUI	CGCG	2 cut(s)	342, 1886
BstV1I	GCAGC	7 cut(s)	95, 164, 353, 789, 862, 1173, 1731
BstV2I	GAAGAC	1 cut(s)	426
BstX2I	RGATCY	4 cut(s)	114, 653, 1004, 1621
BstXI	CCANNNNNNTGG	1 cut(s)	1260
BstYI	RGATCY	4 cut(s)	114, 653, 1004, 1621
BstZI	CGGCCG	1 cut(s)	1748
BsuRI	GGCC	6 cut(s)	348, 610, 962, 1173, 1307, 1750
BtgI	CCRYGG	1 cut(s)	1884
BtsCI	GGATG	1 cut(s)	1042
BtsI	GCAGTG	3 cut(s)	593, 1449, 1877
Cac8I	GCNNGC	6 cut(s)	199, 350, 1462, 1731, 1752, 1884
CaiI	CAGNNNCTG	1 cut(s)	746
CciI	TCATGA	2 cut(s)	976, 1219
CfoI	GCGC	4 cut(s)	56, 517, 586, 1838
Cfr10I	RCCGGY	2 cut(s)	348, 1750
Cfr13I	GGNCC	4 cut(s)	346, 426, 608, 1172
Cfr42I	CCGCGG	1 cut(s)	1887
CseI	GACGC	3 cut(s)	190, 644, 780
Csp6I	GTAC	1 cut(s)	1841
CviAII	CATG	7 cut(s)	157, 250, 977, 1220, 1477, 1504, 1918
CviQI	GTAC	1 cut(s)	1841
DdeI	CTNAG	7 cut(s)	148, 717, 1012, 1256, 1296, 1358, 1800
DinI	GGCGCC	1 cut(s)	516
DpnI	GATC	9 cut(s)	116, 655, 1006, 1092, 1118, 1337, 1623, 1659, 1922
DpnII	GATC	9 cut(s)	114, 653, 1004, 1090, 1116, 1335, 1621, 1657, 1920
DraI	TTTAAA	1 cut(s)	1146
EaeI	YGGCCR	1 cut(s)	1748
EagI	CGGCCG	1 cut(s)	1748
Ecl136II	GAGCTC	1 cut(s)	902
EclXI	CGGCCG	1 cut(s)	1748
Eco24I	GRGCYC	1 cut(s)	904
Eco47I	GGWCC	1 cut(s)	426
Eco47III	AGCGCT	1 cut(s)	55
Eco52I	CGGCCG	1 cut(s)	1748
Eco53kI	GAGCTC	1 cut(s)	902
Eco57I	CTGAAG	3 cut(s)	1449, 1719, 1845
Eco88I	CYCGRG	3 cut(s)	67, 953, 1175
EcoICRI	GAGCTC	1 cut(s)	902
EcoO109I	RGGNCCY	2 cut(s)	608, 1172
EcoRI	GAATTC	2 cut(s)	1125, 1382
EcoRII	CCWGG	2 cut(s)	610, 1616
EcoT38I	GRGCYC	1 cut(s)	904
EgeI	GGCGCC	1 cut(s)	516
EheI	GGCGCC	1 cut(s)	516
Esp3I	CGTCTC	1 cut(s)	265
FaeI	CATG	7 cut(s)	160, 253, 980, 1223, 1480, 1507, 1921
FalI	AAGNNNNNCTT	2 cut(s)	1582, 1614
FaqI	GGGAC	3 cut(s)	452, 910, 1187
FatI	CATG	7 cut(s)	156, 249, 976, 1219, 1476, 1503, 1917
FauI	CCCGC	2 cut(s)	335, 1891
FbaI	TGATCA	1 cut(s)	1116
Fnu4HI	GCNGC	9 cut(s)	84, 153, 367, 775, 778, 876, 1162, 1745, 1748
FokI	GGATG	1 cut(s)	1049
FriOI	GRGCYC	1 cut(s)	904
Fsp4HI	GCNGC	9 cut(s)	84, 153, 367, 775, 778, 876, 1162, 1745, 1748
FspBI	CTAG	3 cut(s)	1416, 1469, 1667
GlaI	GCGC	4 cut(s)	55, 516, 585, 1837
GluI	GCNGC	9 cut(s)	84, 153, 367, 775, 778, 876, 1162, 1745, 1748
GsaI	CCCAGC	2 cut(s)	543, 696
HaeII	RGCGCY	2 cut(s)	57, 518
HaeIII	GGCC	6 cut(s)	348, 610, 962, 1173, 1307, 1750
HapII	CCGG	7 cut(s)	210, 216, 349, 376, 1034, 1484, 1751
HgaI	GACGC	3 cut(s)	190, 644, 780
HhaI	GCGC	4 cut(s)	56, 517, 586, 1838
Hin1I	GRCGYC	4 cut(s)	258, 515, 636, 772
Hin1II	CATG	7 cut(s)	160, 253, 980, 1223, 1480, 1507, 1921
Hin6I	GCGC	4 cut(s)	54, 515, 584, 1836
HinP1I	GCGC	4 cut(s)	54, 515, 584, 1836
HincII	GTYRAC	1 cut(s)	1509
HindII	GTYRAC	1 cut(s)	1509
HindIII	AAGCTT	2 cut(s)	989, 1731
HinfI	GANTC	6 cut(s)	457, 648, 715, 1322, 1795, 1889
HpaII	CCGG	7 cut(s)	210, 216, 349, 376, 1034, 1484, 1751
HphI	GGTGA	4 cut(s)	160, 199, 505, 566
Hpy166II	GTNNAC	3 cut(s)	574, 1509, 1528
Hpy8I	GTNNAC	3 cut(s)	574, 1509, 1528
Hpy99I	CGWCG	2 cut(s)	206, 365
HpyCH4III	ACNGT	6 cut(s)	229, 430, 466, 742, 916, 1532
HpyCH4IV	ACGT	4 cut(s)	124, 222, 258, 564
HpyCH4V	TGCA	9 cut(s)	96, 249, 297, 660, 755, 845, 1161, 1391, 1729
HpyF10VI	GCNNNNNNNGC	9 cut(s)	80, 161, 282, 689, 842, 908, 1170, 1179, 1397
HpyF3I	CTNAG	7 cut(s)	148, 717, 1012, 1256, 1296, 1358, 1800
HpySE526I	ACGT	4 cut(s)	124, 222, 258, 564
Hsp92I	GRCGYC	4 cut(s)	258, 515, 636, 772
Hsp92II	CATG	7 cut(s)	160, 253, 980, 1223, 1480, 1507, 1921
HspAI	GCGC	4 cut(s)	54, 515, 584, 1836
KasI	GGCGCC	1 cut(s)	514
Kpn2I	TCCGGA	1 cut(s)	1483
KroI	GCCGGC	2 cut(s)	348, 1750
KroNI	GCCGGC	2 cut(s)	350, 1752
Ksp22I	TGATCA	1 cut(s)	1116
KspI	CCGCGG	1 cut(s)	1887
Kzo9I	GATC	9 cut(s)	114, 653, 1004, 1090, 1116, 1335, 1621, 1657, 1920
LmnI	GCTCC	4 cut(s)	30, 899, 1249, 1486
Lsp1109I	GCAGC	7 cut(s)	95, 164, 353, 789, 862, 1173, 1731
LweI	GCATC	3 cut(s)	742, 970, 1703
MaeI	CTAG	3 cut(s)	1416, 1469, 1667
MaeII	ACGT	4 cut(s)	124, 222, 258, 564
MaeIII	GTNAC	8 cut(s)	166, 205, 223, 742, 799, 1472, 1701, 1716
MalI	GATC	9 cut(s)	116, 655, 1006, 1092, 1118, 1337, 1623, 1659, 1922
MboI	GATC	9 cut(s)	114, 653, 1004, 1090, 1116, 1335, 1621, 1657, 1920
MflI	RGATCY	4 cut(s)	114, 653, 1004, 1621
MhlI	GDGCHC	1 cut(s)	904
Mly113I	GGCGCC	1 cut(s)	515
MlyI	GAGTC	2 cut(s)	709, 1898
MmeI	TCCRAC	2 cut(s)	165, 434
MroI	TCCGGA	1 cut(s)	1483
MroNI	GCCGGC	2 cut(s)	348, 1750
MseI	TTAA	4 cut(s)	471, 642, 1110, 1145
MslI	CAYNNNNRTG	1 cut(s)	1258
MspA1I	CMGCKG	3 cut(s)	1122, 1295, 1886
MspI	CCGG	7 cut(s)	210, 216, 349, 376, 1034, 1484, 1751
MspR9I	CCNGG	2 cut(s)	612, 1618
Mva1269I	GAATGC	1 cut(s)	694
MvaI	CCWGG	2 cut(s)	612, 1618
MvnI	CGCG	2 cut(s)	342, 1886
MwoI	GCNNNNNNNGC	9 cut(s)	80, 161, 282, 689, 842, 908, 1170, 1179, 1397
NaeI	GCCGGC	2 cut(s)	350, 1752
NarI	GGCGCC	1 cut(s)	515
NdeII	GATC	9 cut(s)	114, 653, 1004, 1090, 1116, 1335, 1621, 1657, 1920
NgoMIV	GCCGGC	2 cut(s)	348, 1750
NlaIII	CATG	7 cut(s)	160, 253, 980, 1223, 1480, 1507, 1921
NlaIV	GGNNCC	8 cut(s)	214, 317, 347, 516, 609, 1174, 1251, 1482
NmeAIII	GCCGAG	1 cut(s)	543
NmuCI	GTSAC	3 cut(s)	166, 205, 742
NspI	RCATGY	1 cut(s)	1507
NspV	TTCGAA	1 cut(s)	1907
OliI	CACNNNNGTG	1 cut(s)	1258
PaeR7I	CTCGAG	1 cut(s)	953
PagI	TCATGA	2 cut(s)	976, 1219
PcsI	WCGNNNNNNNCGW	6 cut(s)	201, 240, 387, 515, 951, 1904
PctI	GAATGC	1 cut(s)	694
PdiI	GCCGGC	2 cut(s)	350, 1752
PfeI	GAWTC	4 cut(s)	457, 648, 1322, 1795
PkrI	GCNGC	9 cut(s)	85, 154, 368, 776, 779, 877, 1163, 1746, 1749
PleI	GAGTC	2 cut(s)	709, 1897
PluTI	GGCGCC	1 cut(s)	518
PpsI	GAGTC	2 cut(s)	709, 1897
Psp124BI	GAGCTC	1 cut(s)	904
Psp1406I	AACGTT	1 cut(s)	564
Psp6I	CCWGG	2 cut(s)	610, 1616
PspFI	CCCAGC	2 cut(s)	539, 692
PspGI	CCWGG	2 cut(s)	610, 1616
PspN4I	GGNNCC	8 cut(s)	214, 317, 347, 516, 609, 1174, 1251, 1482
PspPI	GGNCC	4 cut(s)	346, 426, 608, 1172
PstNI	CAGNNNCTG	1 cut(s)	746
PsuI	RGATCY	4 cut(s)	114, 653, 1004, 1621
PvuII	CAGCTG	1 cut(s)	1295
RsaI	GTAC	1 cut(s)	1842
RsaNI	GTAC	1 cut(s)	1841
RseI	CAYNNNNRTG	1 cut(s)	1258
SacI	GAGCTC	1 cut(s)	904
SacII	CCGCGG	1 cut(s)	1887
SaqAI	TTAA	4 cut(s)	471, 642, 1110, 1145
SatI	GCNGC	9 cut(s)	84, 153, 367, 775, 778, 876, 1162, 1745, 1748
Sau3AI	GATC	9 cut(s)	114, 653, 1004, 1090, 1116, 1335, 1621, 1657, 1920
Sau96I	GGNCC	4 cut(s)	346, 426, 608, 1172
ScaI	AGTACT	1 cut(s)	1842
SchI	GAGTC	2 cut(s)	709, 1898
ScrFI	CCNGG	2 cut(s)	612, 1618
SduI	GDGCHC	1 cut(s)	904
SfaNI	GCATC	3 cut(s)	742, 970, 1703
SfoI	GGCGCC	1 cut(s)	516
Sfr274I	CTCGAG	1 cut(s)	953
Sfr303I	CCGCGG	1 cut(s)	1887
SfuI	TTCGAA	1 cut(s)	1907
SgrBI	CCGCGG	1 cut(s)	1887
SinI	GGWCC	1 cut(s)	426
SlaI	CTCGAG	1 cut(s)	953
SmiMI	CAYNNNNRTG	1 cut(s)	1258
SmlI	CTYRAG	3 cut(s)	409, 953, 1790
SmoI	CTYRAG	3 cut(s)	409, 953, 1790
SsiI	CCGC	7 cut(s)	342, 775, 830, 1122, 1747, 1884, 1886
SspDI	GGCGCC	1 cut(s)	514
SspMI	CTAG	3 cut(s)	1416, 1469, 1667
SstI	GAGCTC	1 cut(s)	904
StyD4I	CCNGG	2 cut(s)	610, 1616
TaaI	ACNGT	6 cut(s)	229, 430, 466, 742, 916, 1532
TaiI	ACGT	4 cut(s)	127, 225, 261, 567
TaqI	TCGA	9 cut(s)	360, 390, 713, 954, 1325, 1551, 1595, 1656, 1907
TaqII	GACCGA	1 cut(s)	1526
TatI	WGTACW	1 cut(s)	1840
TauI	GCSGC	2 cut(s)	777, 1750
TfiI	GAWTC	4 cut(s)	457, 648, 1322, 1795
Tru1I	TTAA	4 cut(s)	471, 642, 1110, 1145
Tru9I	TTAA	4 cut(s)	471, 642, 1110, 1145
TseFI	GTSAC	3 cut(s)	166, 205, 742
TseI	GCWGC	7 cut(s)	83, 152, 366, 777, 875, 1161, 1744
Tsp45I	GTSAC	3 cut(s)	166, 205, 742
TspDTI	ATGAA	9 cut(s)	124, 173, 266, 930, 965, 1118, 1208, 1331, 1701
TspGWI	ACGGA	1 cut(s)	284
VpaK11BI	GGWCC	1 cut(s)	426
XapI	RAATTY	3 cut(s)	499, 1125, 1382
XceI	RCATGY	1 cut(s)	1507
XhoI	CTCGAG	1 cut(s)	953
XspI	CTAG	3 cut(s)	1416, 1469, 1667
ZraI	GACGTC	1 cut(s)	259
ZrmI	AGTACT	1 cut(s)	1842

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.