Rw0G020160

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00906
OG0005807
Physical Location & Seq
Forward (+)
56316 .. 59271
2956 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G020160.1

Sequence Viewer

Length: 1947 bp
ATGTCTAATTGCATCCCAGTTCTGCTCATTTCTCTCTTGTTTCTGTTACTCAACTCCGCTCTGGCCGATCCACGAGCGACAGAGGCGGCTCTGATATGTACAAACGACACCGCTTTGTTCGCACAGAGACAGTCCTTCATAGCCAACTTCCTGGCAGCGCTGGACACGCTGACCCCTCTCATTTCCCGCCAAAAGTACGCTGCCGTCGTCAATGGCACCGGAAACACCACGGTATACGCTTTCGGCGAGTGCATGAGAGATCTTTCTCAAACAGACTGCAACCTCTGCTTTGCTCAGTGCAAGACACAGATTCTGAGGTGCCTGCCGTTTCAGAAGGCCACTCGTGGGGGTAGGCTTTTCTTCGACGGGTGTTATCTCCGGTACGACGACTTCAATTTCTTTAATCAGAGTTTGAGCGCCGAAGACACGAGTGTCTGTGGGGTTAACGATTTTGGTGGGAATCGAACAGTTTTTAAAGATAATGCATTGGAATTGGTGAAGAATTTGAGCGTGGAAGCTGCCAGGAATGATGGGTTTTCTGTTGGGTCTGTGACTCGGCAGAGTGTAACGGTTTATGGGTTGGCTCAGTGTTGGAAATTACTGAATGGATCTGCTTGTCATAAGTGTTTGAGTGACACAGTCATTCGAATCGGGTCGTGCCCTCCGAAAGAAGAAGGGAGGGCGTTGAATGCTGGCTGTTACTTGAGGTATTCCACCCAGAAGTTTTATAATAACAATACGGTGACGGATGGAGGAGGAAATGGAGGACACCATCGCTTGGCTATAATTGTGGGTATAACATCTGCTGCATTTGCTCTTGTGCTGGCTATTTCCGCCTTTGCTTTCTTGACAAGGAAGAAATTGTTGGAGAAGAGGAAAGAGAAAAAGCAACTAGGTGCTCTGTTTTCCATTGTGAACAAGTCCCAACTCAATTATTCATATGAAGTTCTTGAAAATGCCACCAATTACTTCCATGATTCTAACAAGCTTGGACAAGGAGGATCTGGTTCAGTTTACAAAGGAGTTTTGTCAGATGGAAAGATTGTTGCCATAAAGAGGTTATTCTTCAATACAAGACAATGGGTGGATCATTTCTTCAATGAAGTCAATTTGATCAGTGGAATCCATCACAAAAATCTTGTAAAGCTATTGGGATGCAGCATTACTGGCCCTGAAAGCCTTCTTGTTTATGAGTATCTTCCAAATCAAAGCCTTCATGACTATTTTAGTGTGAAAAATAATCTTGAGCCACTAAGGTGGGAGTTGAGGTATAAGATCATATTGGGGACAGCTGAGGGCTTGGCCTATCTTCATGAAGAATCAAAAGTGAGAATTATTCATAGGGATATAAAGTTAAGCAACATTCTGCTGGATGAGAACTTCATGCCTAAGATTGCCGATTTTGGACTCGCTAGGTTGTTTCCTGAAGATAAAACTCACATTAGCACTGCCATTGCCGGTACATTAGGTTATATGGCTCCCGAGTATGTCACTCGTGGCAAGTTGACGGAGAAGGCAGACGTTTACAGTTTTGGAGTTCTTGTCATTGAAATCATATCCGGAAAGAGGATCAATAGCTTTGCATCAAATTATGTTTCTATCCTACAGACGGTTTGGGACTTGTATGGAACGGGTCAGCTATGTAAGGCAGTTGATCTAGTTCTAGAGGGTCAGTTCATGAATAGTGAAGCATCTCAATTACTTCAAGTTGGACTACTTTGTGTACAAGCCTCTGCAGAGCTGCGACCATCAATGTCATTAGTAGTGAAAATGCTCACTGATCAGAATCTTGAAATTCCTCAGCCGACTCAGCCACCATTTCTCAATTCTGGGAGTGCAGAATACAGCAGGCATAATATTCCTTCTGGGACTTCCAAATCACAGCCCGAGTCTCACACACATTCTTCAAGGAATAGTATGACCCAAAGCTGGATTGAACCTAGATGA

Protein Analysis

648

Amino Acids

71.76

Weight (kDa)

8.52

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 33 - 130 2.2e-12 Salt stress response/antifungal
Stress-antifung PF01657 153 - 240 4e-14 Salt stress response/antifungal
Pkinase PF00069 324 - 537 6.4e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 327 - 591 3e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
AasI GACNNNNNNGTC 1 cut(s) 431
AccB1I GGYRCC 2 cut(s) 215, 318
AccB7I CCANNNNNTGG 1 cut(s) 778
AccBSI CCGCTC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 234
AccIII TCCGGA 1 cut(s) 1559
AciI CCGC 5 cut(s) 57, 86, 111, 187, 834
AclWI GGATC 5 cut(s) 62, 616, 1009, 1095, 1577
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 2 cut(s) 502, 1794
AcuI CTGAAG 1 cut(s) 1446
AfaI GTAC 5 cut(s) 100, 197, 383, 1462, 1725
AfeI AGCGCT 1 cut(s) 159
AfiI CCNNNNNNNGG 6 cut(s) 345, 778, 1056, 1566, 1609, 1929
AjnI CCWGG 2 cut(s) 150, 521
AjuI GAANNNNNNNTTGG 2 cut(s) 848, 880
AleI CACNNNNGTG 1 cut(s) 1255
AluBI AGCT 8 cut(s) 518, 988, 1147, 1292, 1578, 1639, 1741, 1929
AluI AGCT 8 cut(s) 518, 988, 1147, 1292, 1578, 1639, 1741, 1929
Alw21I GWGCWC 1 cut(s) 901
Alw26I GTCTC 2 cut(s) 121, 1896
AlwI GGATC 5 cut(s) 62, 616, 1009, 1095, 1577
AlwNI CAGNNNCTG 1 cut(s) 313
Ama87I CYCGRG 2 cut(s) 1481, 1886
Aor13HI TCCGGA 1 cut(s) 1559
Aor51HI AGCGCT 1 cut(s) 159
AoxI GGCC 4 cut(s) 63, 336, 1168, 1302
ApeKI GCWGC 6 cut(s) 155, 200, 518, 806, 1158, 1741
ApoI RAATTY 2 cut(s) 502, 1794
Asp700I GAANNNNTTC 1 cut(s) 1179
AspLEI GCGC 2 cut(s) 160, 419
AspS9I GGNCC 1 cut(s) 1169
AsuHPI GGTGA 2 cut(s) 508, 754
AsuII TTCGAA 1 cut(s) 646
AvaI CYCGRG 2 cut(s) 1481, 1886
BaeGI GKGCMC 1 cut(s) 662
BanI GGYRCC 2 cut(s) 215, 318
BarI GAAGNNNNNNTAC 2 cut(s) 374, 406
BauI CACGAG 4 cut(s) 72, 342, 427, 1494
BbsI GAAGAC 1 cut(s) 429
Bbv12I GWGCWC 1 cut(s) 901
BbvCI CCTCAGC 2 cut(s) 1293, 1800
BbvI GCAGC 6 cut(s) 167, 187, 505, 793, 1170, 1728
BccI CCATC 6 cut(s) 524, 743, 780, 1028, 1134, 1756
BceAI ACGGC 2 cut(s) 188, 310
BciT130I CCWGG 2 cut(s) 152, 523
BclI TGATCA 2 cut(s) 1113, 1780
BcoDI GTCTC 2 cut(s) 121, 1896
BfaI CTAG 5 cut(s) 893, 1413, 1658, 1664, 1941
BfmI CTRYAG 2 cut(s) 1604, 1734
BfoI RGCGCY 2 cut(s) 161, 420
BglII AGATCT 1 cut(s) 259
BisI GCNGC 7 cut(s) 87, 156, 201, 519, 807, 1159, 1742
BlsI GCNGC 7 cut(s) 88, 157, 202, 520, 808, 1160, 1743
Bme1390I CCNGG 2 cut(s) 152, 523
BmeT110I CYCGRG 2 cut(s) 1481, 1886
BmgT120I GGNCC 1 cut(s) 1169
BmiI GGNNCC 3 cut(s) 217, 320, 1479
BmrFI CCNGG 2 cut(s) 152, 523
BmrI ACTGGG 1 cut(s) 11
BmsI GCATC 4 cut(s) 21, 1145, 1592, 1700
BmuI ACTGGG 1 cut(s) 11
BpiI GAAGAC 1 cut(s) 429
Bpu10I CCTNAGC 2 cut(s) 1293, 1800
Bpu14I TTCGAA 1 cut(s) 646
BpuEI CTTGAG 2 cut(s) 724, 1265
BsaBI GATNNNNATC 1 cut(s) 1785
BsaJI CCNNGG 1 cut(s) 228
BsaWI WCCGGW 3 cut(s) 218, 378, 1559
BsaXI ACNNNNNCTCC 4 cut(s) 1253, 1283, 1527, 1557
Bsc4I CCNNNNNNNGG 6 cut(s) 345, 778, 1056, 1566, 1609, 1929
Bse118I RCCGGY 1 cut(s) 1457
Bse1I ACTGG 2 cut(s) 17, 1171
Bse3DI GCAATG 1 cut(s) 1452
Bse8I GATNNNNATC 1 cut(s) 1785
BseAI TCCGGA 1 cut(s) 1559
BseBI CCWGG 2 cut(s) 152, 523
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 4 cut(s) 12, 754, 1160, 1378
BseJI GATNNNNATC 1 cut(s) 1785
BseLI CCNNNNNNNGG 6 cut(s) 345, 778, 1056, 1566, 1609, 1929
BseMI GCAATG 1 cut(s) 1452
BseMII CTCAG 6 cut(s) 305, 308, 599, 1284, 1814, 1823
BseNI ACTGG 2 cut(s) 17, 1171
BseRI GAGGAG 1 cut(s) 768
BseSI GKGCMC 1 cut(s) 662
BseXI GCAGC 6 cut(s) 167, 187, 505, 793, 1170, 1728
BsgI GTGCAG 1 cut(s) 1857
BshFI GGCC 4 cut(s) 65, 338, 1170, 1304
BshNI GGYRCC 2 cut(s) 215, 318
BsiHKAI GWGCWC 1 cut(s) 901
BsiHKCI CYCGRG 2 cut(s) 1481, 1886
BsiSI CCGG 4 cut(s) 219, 379, 1458, 1560
BslFI GGGAC 4 cut(s) 907, 1300, 1631, 1882
BslI CCNNNNNNNGG 6 cut(s) 345, 778, 1056, 1566, 1609, 1929
BsmAI GTCTC 2 cut(s) 121, 1896
BsmFI GGGAC 4 cut(s) 907, 1300, 1631, 1882
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 4 cut(s) 65, 338, 1170, 1304
BsoBI CYCGRG 2 cut(s) 1481, 1886
Bsp119I TTCGAA 1 cut(s) 646
Bsp1286I GDGCHC 2 cut(s) 662, 901
Bsp13I TCCGGA 1 cut(s) 1559
Bsp1407I TGTACA 2 cut(s) 98, 1723
BspACI CCGC 5 cut(s) 57, 86, 111, 187, 834
BspANI GGCC 4 cut(s) 65, 338, 1170, 1304
BspCNI CTCAG 6 cut(s) 306, 307, 598, 1285, 1813, 1822
BspEI TCCGGA 1 cut(s) 1559
BspHI TCATGA 3 cut(s) 1216, 1312, 1677
BspLI GGNNCC 3 cut(s) 217, 320, 1479
BspMAI CTGCAG 1 cut(s) 1738
BspPI GGATC 5 cut(s) 62, 616, 1009, 1095, 1577
BspT104I TTCGAA 1 cut(s) 646
BspT107I GGYRCC 2 cut(s) 215, 318
BsrBI CCGCTC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 1452
BsrFI RCCGGY 1 cut(s) 1457
BsrGI TGTACA 2 cut(s) 98, 1723
BsrI ACTGG 2 cut(s) 17, 1171
BssAI RCCGGY 1 cut(s) 1457
BssECI CCNNGG 1 cut(s) 228
BssNAI GTATAC 1 cut(s) 235
BssSI CACGAG 4 cut(s) 72, 342, 427, 1494
Bst1107I GTATAC 1 cut(s) 235
Bst2BI CACGAG 4 cut(s) 72, 342, 427, 1494
Bst2UI CCWGG 2 cut(s) 152, 523
Bst4CI ACNGT 8 cut(s) 132, 232, 469, 571, 640, 742, 1529, 1612
Bst6I CTCTTC 1 cut(s) 866
BstAPI GCANNNNNTGC 1 cut(s) 285
BstAUI TGTACA 2 cut(s) 98, 1723
BstBI TTCGAA 1 cut(s) 646
BstC8I GCNNGC 4 cut(s) 323, 694, 825, 1850
BstDEI CTNAG 8 cut(s) 294, 314, 585, 1253, 1293, 1389, 1800, 1809
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 4 cut(s) 12, 754, 1160, 1378
BstH2I RGCGCY 2 cut(s) 161, 420
BstHHI GCGC 2 cut(s) 160, 419
BstMAI GTCTC 2 cut(s) 121, 1896
BstNI CCWGG 2 cut(s) 152, 523
BstSCI CCNGG 2 cut(s) 150, 521
BstSFI CTRYAG 2 cut(s) 1604, 1734
BstSLI GKGCMC 1 cut(s) 662
BstV1I GCAGC 6 cut(s) 167, 187, 505, 793, 1170, 1728
BstV2I GAAGAC 1 cut(s) 429
BstX2I RGATCY 3 cut(s) 259, 608, 1001
BstXI CCANNNNNNTGG 2 cut(s) 151, 1257
BstYI RGATCY 3 cut(s) 259, 608, 1001
BstZ17I GTATAC 1 cut(s) 235
BsuRI GGCC 4 cut(s) 65, 338, 1170, 1304
BtgI CCRYGG 1 cut(s) 228
BtgZI GCGATG 1 cut(s) 758
BtsCI GGATG 4 cut(s) 12, 754, 1160, 1378
BtsI GCAGTG 1 cut(s) 1446
BtsIMutI CAGTG 5 cut(s) 302, 593, 1123, 1446, 1776
Cac8I GCNNGC 4 cut(s) 323, 694, 825, 1850
CaiI CAGNNNCTG 1 cut(s) 313
CciI TCATGA 3 cut(s) 1216, 1312, 1677
CfoI GCGC 2 cut(s) 160, 419
Cfr10I RCCGGY 1 cut(s) 1457
Cfr13I GGNCC 1 cut(s) 1169
Csp6I GTAC 5 cut(s) 99, 196, 382, 1461, 1724
CviAII CATG 6 cut(s) 253, 974, 1217, 1313, 1384, 1678
CviQI GTAC 5 cut(s) 99, 196, 382, 1461, 1724
DdeI CTNAG 8 cut(s) 294, 314, 585, 1253, 1293, 1389, 1800, 1809
DraI TTTAAA 1 cut(s) 475
DrdI GACNNNNNNGTC 1 cut(s) 431
DseDI GACNNNNNNGTC 1 cut(s) 431
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 866
EarI CTCTTC 1 cut(s) 866
EciI GGCGGA 1 cut(s) 823
Eco47III AGCGCT 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 1446
Eco88I CYCGRG 2 cut(s) 1481, 1886
EcoRII CCWGG 2 cut(s) 150, 521
EcoT22I ATGCAT 1 cut(s) 487
FaeI CATG 6 cut(s) 256, 977, 1220, 1316, 1387, 1681
FaqI GGGAC 4 cut(s) 907, 1300, 1631, 1882
FatI CATG 6 cut(s) 252, 973, 1216, 1312, 1383, 1677
FauI CCCGC 1 cut(s) 194
FauNDI CATATG 1 cut(s) 940
FbaI TGATCA 2 cut(s) 1113, 1780
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 7 cut(s) 87, 156, 201, 519, 807, 1159, 1742
FokI GGATG 3 cut(s) 761, 1167, 1385
Fsp4HI GCNGC 7 cut(s) 87, 156, 201, 519, 807, 1159, 1742
FspBI CTAG 5 cut(s) 893, 1413, 1658, 1664, 1941
GlaI GCGC 2 cut(s) 159, 418
GluI GCNGC 7 cut(s) 87, 156, 201, 519, 807, 1159, 1742
HaeII RGCGCY 2 cut(s) 161, 420
HaeIII GGCC 4 cut(s) 65, 338, 1170, 1304
HapII CCGG 4 cut(s) 219, 379, 1458, 1560
HhaI GCGC 2 cut(s) 160, 419
Hin1II CATG 6 cut(s) 256, 977, 1220, 1316, 1387, 1681
Hin6I GCGC 2 cut(s) 158, 417
HinP1I GCGC 2 cut(s) 158, 417
HincII GTYRAC 2 cut(s) 445, 1506
HindII GTYRAC 2 cut(s) 445, 1506
HindIII AAGCTT 1 cut(s) 986
HpaI GTTAAC 1 cut(s) 445
HpaII CCGG 4 cut(s) 219, 379, 1458, 1560
HphI GGTGA 2 cut(s) 508, 754
Hpy166II GTNNAC 7 cut(s) 235, 445, 916, 1015, 1506, 1525, 1724
Hpy188I TCNGA 7 cut(s) 93, 315, 333, 408, 666, 1033, 1785
Hpy8I GTNNAC 7 cut(s) 235, 445, 916, 1015, 1506, 1525, 1724
Hpy99I CGWCG 3 cut(s) 209, 368, 389
HpyAV CCTTC 7 cut(s) 145, 328, 668, 1190, 1223, 1507, 1872
HpyCH4III ACNGT 8 cut(s) 132, 232, 469, 571, 640, 742, 1529, 1612
HpyCH4IV ACGT 1 cut(s) 1521
HpyF3I CTNAG 8 cut(s) 294, 314, 585, 1253, 1293, 1389, 1800, 1809
HpySE526I ACGT 1 cut(s) 1521
Hsp92II CATG 6 cut(s) 256, 977, 1220, 1316, 1387, 1681
HspAI GCGC 2 cut(s) 158, 417
Kpn2I TCCGGA 1 cut(s) 1559
Ksp22I TGATCA 2 cut(s) 1113, 1780
KspAI GTTAAC 1 cut(s) 445
LmnI GCTCC 1 cut(s) 1483
Lsp1109I GCAGC 6 cut(s) 167, 187, 505, 793, 1170, 1728
LweI GCATC 4 cut(s) 21, 1145, 1592, 1700
MaeI CTAG 5 cut(s) 893, 1413, 1658, 1664, 1941
MaeII ACGT 1 cut(s) 1521
MaeIII GTNAC 7 cut(s) 45, 550, 565, 632, 698, 742, 1489
MbiI CCGCTC 1 cut(s) 59
MflI RGATCY 3 cut(s) 259, 608, 1001
MhlI GDGCHC 2 cut(s) 662, 901
MlyI GAGTC 4 cut(s) 547, 1401, 1801, 1898
MmeI TCCRAC 3 cut(s) 572, 846, 1690
Mph1103I ATGCAT 1 cut(s) 487
MroI TCCGGA 1 cut(s) 1559
MroXI GAANNNNTTC 1 cut(s) 1179
MseI TTAA 4 cut(s) 402, 444, 474, 1355
MslI CAYNNNNRTG 1 cut(s) 1255
MspA1I CMGCKG 1 cut(s) 1292
MspI CCGG 4 cut(s) 219, 379, 1458, 1560
MspR9I CCNGG 2 cut(s) 152, 523
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 2 cut(s) 152, 523
NdeI CATATG 1 cut(s) 940
NlaIII CATG 6 cut(s) 256, 977, 1220, 1316, 1387, 1681
NlaIV GGNNCC 3 cut(s) 217, 320, 1479
NmeAIII GCCGAG 1 cut(s) 535
NmuCI GTSAC 4 cut(s) 550, 632, 742, 1489
NsiI ATGCAT 1 cut(s) 487
NspV TTCGAA 1 cut(s) 646
OliI CACNNNNGTG 1 cut(s) 1255
PagI TCATGA 3 cut(s) 1216, 1312, 1677
PcsI WCGNNNNNNNCGW 3 cut(s) 204, 243, 662
PctI GAATGC 1 cut(s) 694
PdmI GAANNNNTTC 1 cut(s) 1179
PfeI GAWTC 7 cut(s) 310, 460, 648, 977, 1122, 1319, 1786
PflFI GACNNNGTC 1 cut(s) 638
PflMI CCANNNNNTGG 1 cut(s) 778
PkrI GCNGC 7 cut(s) 88, 157, 202, 520, 808, 1160, 1743
PleI GAGTC 4 cut(s) 547, 1401, 1801, 1897
PpsI GAGTC 4 cut(s) 547, 1401, 1801, 1897
PsiI TTATAA 1 cut(s) 729
Psp6I CCWGG 2 cut(s) 150, 521
PspGI CCWGG 2 cut(s) 150, 521
PspN4I GGNNCC 3 cut(s) 217, 320, 1479
PspPI GGNCC 1 cut(s) 1169
PstI CTGCAG 1 cut(s) 1738
PstNI CAGNNNCTG 1 cut(s) 313
PsuI RGATCY 3 cut(s) 259, 608, 1001
PsyI GACNNNGTC 1 cut(s) 638
PvuII CAGCTG 1 cut(s) 1292
RsaI GTAC 5 cut(s) 100, 197, 383, 1462, 1725
RsaNI GTAC 5 cut(s) 99, 196, 382, 1461, 1724
RseI CAYNNNNRTG 1 cut(s) 1255
SaqAI TTAA 4 cut(s) 402, 444, 474, 1355
SatI GCNGC 7 cut(s) 87, 156, 201, 519, 807, 1159, 1742
Sau96I GGNCC 1 cut(s) 1169
SchI GAGTC 4 cut(s) 547, 1401, 1801, 1898
ScrFI CCNGG 2 cut(s) 152, 523
SduI GDGCHC 2 cut(s) 662, 901
SfaNI GCATC 4 cut(s) 21, 1145, 1592, 1700
SfcI CTRYAG 2 cut(s) 1604, 1734
SfuI TTCGAA 1 cut(s) 646
SmiMI CAYNNNNRTG 1 cut(s) 1255
SmlI CTYRAG 2 cut(s) 703, 1244
SmoI CTYRAG 2 cut(s) 703, 1244
SsiI CCGC 5 cut(s) 57, 86, 111, 187, 834
SspI AATATT 1 cut(s) 1858
SspMI CTAG 5 cut(s) 893, 1413, 1658, 1664, 1941
StyD4I CCNGG 2 cut(s) 150, 521
TaaI ACNGT 8 cut(s) 132, 232, 469, 571, 640, 742, 1529, 1612
TaiI ACGT 1 cut(s) 1524
TaqI TCGA 3 cut(s) 363, 463, 646
TatI WGTACW 2 cut(s) 98, 1723
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 7 cut(s) 310, 460, 648, 977, 1122, 1319, 1786
Tru1I TTAA 4 cut(s) 402, 444, 474, 1355
Tru9I TTAA 4 cut(s) 402, 444, 474, 1355
TscAI CASTG 5 cut(s) 302, 593, 1123, 1453, 1783
TseFI GTSAC 4 cut(s) 550, 632, 742, 1489
TseI GCWGC 6 cut(s) 155, 200, 518, 806, 1158, 1741
Tsp45I GTSAC 4 cut(s) 550, 632, 742, 1489
TspGWI ACGGA 2 cut(s) 761, 1523
TspRI CASTG 5 cut(s) 302, 593, 1123, 1453, 1783
Tth111I GACNNNGTC 1 cut(s) 638
Van91I CCANNNNNTGG 1 cut(s) 778
XapI RAATTY 2 cut(s) 502, 1794
XbaI TCTAGA 1 cut(s) 1663
XmiI GTMKAC 1 cut(s) 234
XmnI GAANNNNTTC 1 cut(s) 1179
XspI CTAG 5 cut(s) 893, 1413, 1658, 1664, 1941
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.