Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0005807

Physical Location & Seq

Genomic Coordinates Reverse (-)

10166867 .. 10169366

Gene Structure 2500 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000006746

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1872 bp

ATGTCTAATTGCATCCCAGTTCTGCTCATTTCTCTCTTGTTTCTGTTACTCAACTCCGCTCTGGCCGATCCACGAGCGACAGAGGCCGCTCTGATATGTACAAACGACACCGCTTTGGTCGCACAGAGACAGTCCTTCATAGCCAACTTCCTGGCAGCCCTGGACACGCTGACCCCTCTCATTTCCCGCCAAAAGTACGCTGCCGTCGTCAACGGCACCGGAAACACCACCGTATACGCTTTCGGCGAGTGCATGAGAGATCTTTCTCAAACAGACTGCAACCTCTGCTTTGCTCAGTGCAAGACACAGATTCTGAGGTGCCTGCCGTTTCAGAAGGCCACTCGTGGGGGTAGGCTTTTCTACGACGGGTGTTATCTCCGGTACGACGACTTCAATTTCTTTAATCAGAGTTTGAGCGCTGAAGACACGAGTGTCTGTGGGGTTAACGATTTTGGTGGGAATCAAACGGTTTTTAAAGCTAATGCATTGGAATTGGTGAAGAATTTGAGCGTGGAAGCTGCCAAGAATGATGGGTTTTTTGTTGGGTCCGTGACTCGGCAGAATGTAACGGTTTATGGGTTGGCTCAGTGTTGGGAATTACTGAATGGCTCTGCTTGTGAGAAGTGTTTGAGTGACACAGTCACTAGAATCGGGTCCTGCCCTCCGAAAGGAGAAGGGAGGGTCTTGAATGCTGGCTGTTACTTGAGGTATTCCACCCAGAAGTTTTATAATAACAATACGGTGACGGATGGAGGAGGAAATGGAGGACACCGTCGCTTGGCTATAATTGTGGTTATAACATCTGCTGCATTTGCCCTTGTGCTGGCCATTTCCGCCTTTGCTTTCTTGACAAGGAAGAAATTGCTGGAGAAGAGGAAAGAGAAAAAGCAACTAGGTGCTCTGTTTTCCATTGTGAACAAGTCCCAACTCAATTATTCATATGAAGGAGTTTTGTCAGATGGAAAGGTTGTTGCCATAAAGAGGTTATTCTTCAATACAAGACAATGGGTGGATCATTTCTTCAATGAAGTCAATTTGATCAGTGGAATCCATCACAAAAATCTTGTAAAGCTATTGGGATGCAGCATTACTGGCCCTGAAAGCCTTCTTGTTTATGAGTATGTTCCAAATCAAAGCCTTCATGACTATTTTAGTGTGAAAAATAATCTTGAGCCACTGAGGTGGGAGTTGAGGTATAAGATCATATTGGGGACAGCTGAGGGCTTGGCCTATCTTCATGAAGAATCAAAAGTGAGAATTATTCATAGGGATATAAAGTTAAGCAACATTCTGCTGGATGAGAACTTCATGCCGAAGATTGCCGATTTTGGACTCGCTAGGTTGTTTCCTGAAGATAAAACTCACATTAGCACTGCCATTGCCGGTACATTAGGTTATATGGCTCCTGAGTATGTCACTCGTGGCAAGTTGACGGAGAAGGCAGACGTTTACAGTTTTGGAGTTCTTGTCATTGAAATCATATCTGGAAAGAGGATCAATAGCTTTGCATCAAATTCTGTTTCTATCCTACAGATGGTTTGGGACTTGTATGGAACGGGTCAGCTATGTAATGCGGTTGATCCAGTTCTAGAGGGTCAGTTCATAAATAGTGAAGCATCTCAATTACTTCAAGTTGGACTACTTTGTGTTCAAGCCTCTGCAGAGCTGCGACCATCAATGTCATTTGTAGTGAAAATGCTCACTGATCAGAATCTTGAAATTCCTCAGCCGACACAGCCACCATTTCTCAATTCTGGGAGTGCAGAATACAGCAGACATAATATTCCTTCTGGGACTTCCAATTCACAGCCCGAGTCTCACACACATTCTTCAAGGAATAGTATGACCCAAAGCTGGATTGAGCCTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

624

Amino Acids

68.94

Weight (kDa)

8.2

Isoelectric Point (pI)

37.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	33 - 130	9.9e-13	Salt stress response/antifungal
Stress-antifung	PF01657	153 - 240	4.5e-15	Salt stress response/antifungal
Pkinase	PF00069	319 - 560	1.1e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	320 - 565	6.6e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005807)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70530 AT1G70530 AT1G70530
fragaria_vesca	FvH4_4g26070 FvH4_4g26070 FvH4_4g26070 FvH4_4g26070 FvH4_4g26070 FvH4_4g26070 FvH4_4g26070 FvH4_4g26070
malus_domestica	MD13G1096600.v1.1 MD16G1097600.v1.1
prunus_persica	Prupe.1G249300_v2.0.a1
pyrus_communis	pycom13g08420 pycom16g08300
rosa_chinensis	RchiOBHm_Chr4g0433521
rosa_laevigata	RLG00000006746
rosa_multiflora	Rmu_co8500781.1_g000001 Rmu_sc0000312.1_g000008
rosa_roxburghii	Rroxscaffold_5G00375040
rosa_rugosa	Rorug04G0269300
rosa_samantha	Rh4AG324000 Rh4BG332100 Rh4CG347400 Rh4DG328400
rosa_wichuraiana	Rw0G020160 Rw4G028070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	729, 797
AasI	GACNNNNNNGTC	1 cut(s)	431
AccB1I	GGYRCC	2 cut(s)	215, 318
AccBSI	CCGCTC	2 cut(s)	59, 89
AccI	GTMKAC	1 cut(s)	234
AciI	CCGC	6 cut(s)	57, 87, 111, 187, 834, 1574
AclWI	GGATC	4 cut(s)	62, 1020, 1502, 1574
AcoI	YGGCCR	2 cut(s)	63, 825
AcsI	RAATTY	3 cut(s)	502, 1513, 1719
AcuI	CTGAAG	2 cut(s)	441, 1371
AfaI	GTAC	4 cut(s)	100, 197, 383, 1387
AfeI	AGCGCT	1 cut(s)	418
AfiI	CCNNNNNNNGG	8 cut(s)	345, 555, 668, 778, 823, 981, 1534, 1854
AgsI	TTSAA	9 cut(s)	394, 688, 994, 1024, 1475, 1631, 1652, 1718, 1833
AjnI	CCWGG	2 cut(s)	150, 159
AleI	CACNNNNGTG	1 cut(s)	1180
AluBI	AGCT	8 cut(s)	479, 518, 1072, 1217, 1503, 1564, 1666, 1854
AluI	AGCT	8 cut(s)	479, 518, 1072, 1217, 1503, 1564, 1666, 1854
Alw21I	GWGCWC	1 cut(s)	901
Alw26I	GTCTC	2 cut(s)	121, 1821
AlwI	GGATC	4 cut(s)	62, 1020, 1502, 1574
AlwNI	CAGNNNCTG	1 cut(s)	313
Ama87I	CYCGRG	1 cut(s)	1811
Aor51HI	AGCGCT	1 cut(s)	418
AoxI	GGCC	6 cut(s)	63, 84, 336, 825, 1093, 1227
ApeKI	GCWGC	6 cut(s)	155, 200, 518, 806, 1083, 1666
ApoI	RAATTY	3 cut(s)	502, 1513, 1719
Asp700I	GAANNNNTTC	1 cut(s)	1104
AspLEI	GCGC	1 cut(s)	419
AspS9I	GGNCC	3 cut(s)	546, 654, 1094
AsuHPI	GGTGA	2 cut(s)	508, 754
AvaI	CYCGRG	1 cut(s)	1811
AvaII	GGWCC	2 cut(s)	546, 654
BalI	TGGCCA	1 cut(s)	827
BanI	GGYRCC	2 cut(s)	215, 318
BarI	GAAGNNNNNNTAC	2 cut(s)	374, 406
BauI	CACGAG	4 cut(s)	72, 342, 427, 1419
BbsI	GAAGAC	1 cut(s)	429
Bbv12I	GWGCWC	1 cut(s)	901
BbvCI	CCTCAGC	2 cut(s)	1218, 1725
BbvI	GCAGC	6 cut(s)	167, 187, 505, 793, 1095, 1653
BccI	CCATC	6 cut(s)	524, 743, 953, 1059, 1528, 1681
BceAI	ACGGC	3 cut(s)	188, 229, 310
BciT130I	CCWGG	2 cut(s)	152, 161
BclI	TGATCA	2 cut(s)	1038, 1705
BcoDI	GTCTC	2 cut(s)	121, 1821
BfaI	CTAG	5 cut(s)	645, 893, 1338, 1589, 1866
BfmI	CTRYAG	2 cut(s)	1529, 1659
BfoI	RGCGCY	1 cut(s)	420
BglII	AGATCT	1 cut(s)	259
BisI	GCNGC	7 cut(s)	87, 156, 201, 519, 807, 1084, 1667
BlsI	GCNGC	7 cut(s)	88, 157, 202, 520, 808, 1085, 1668
Bme1390I	CCNGG	2 cut(s)	152, 161
Bme18I	GGWCC	2 cut(s)	546, 654
BmeT110I	CYCGRG	1 cut(s)	1811
BmgT120I	GGNCC	3 cut(s)	546, 654, 1094
BmiI	GGNNCC	5 cut(s)	217, 320, 547, 655, 1404
BmrFI	CCNGG	2 cut(s)	152, 161
BmrI	ACTGGG	1 cut(s)	11
BmsI	GCATC	4 cut(s)	21, 1070, 1517, 1625
BmuI	ACTGGG	1 cut(s)	11
BpiI	GAAGAC	1 cut(s)	429
BpmI	CTGGAG	1 cut(s)	887
Bpu10I	CCTNAGC	2 cut(s)	1218, 1725
BpuEI	CTTGAG	2 cut(s)	724, 1190
BsaBI	GATNNNNATC	1 cut(s)	1710
BsaJI	CCNNGG	1 cut(s)	159
BsaWI	WCCGGW	2 cut(s)	218, 378
BsaXI	ACNNNNNCTCC	4 cut(s)	1178, 1208, 1452, 1482
Bsc4I	CCNNNNNNNGG	8 cut(s)	345, 555, 668, 778, 823, 981, 1534, 1854
Bse118I	RCCGGY	1 cut(s)	1382
Bse1I	ACTGG	3 cut(s)	17, 1096, 1583
Bse3DI	GCAATG	1 cut(s)	1377
Bse8I	GATNNNNATC	1 cut(s)	1710
BseBI	CCWGG	2 cut(s)	152, 161
BseDI	CCNNGG	1 cut(s)	159
BseGI	GGATG	4 cut(s)	12, 754, 1085, 1303
BseJI	GATNNNNATC	1 cut(s)	1710
BseLI	CCNNNNNNNGG	8 cut(s)	345, 555, 668, 778, 823, 981, 1534, 1854
BseMI	GCAATG	1 cut(s)	1377
BseMII	CTCAG	7 cut(s)	305, 308, 599, 1169, 1209, 1398, 1739
BseNI	ACTGG	3 cut(s)	17, 1096, 1583
BseRI	GAGGAG	1 cut(s)	768
BseXI	GCAGC	6 cut(s)	167, 187, 505, 793, 1095, 1653
BsgI	GTGCAG	1 cut(s)	1782
BshFI	GGCC	6 cut(s)	65, 86, 338, 827, 1095, 1229
BshNI	GGYRCC	2 cut(s)	215, 318
BsiHKAI	GWGCWC	1 cut(s)	901
BsiHKCI	CYCGRG	1 cut(s)	1811
BsiSI	CCGG	3 cut(s)	219, 379, 1383
BslFI	GGGAC	4 cut(s)	907, 1225, 1556, 1807
BslI	CCNNNNNNNGG	8 cut(s)	345, 555, 668, 778, 823, 981, 1534, 1854
BsmAI	GTCTC	2 cut(s)	121, 1821
BsmFI	GGGAC	4 cut(s)	907, 1225, 1556, 1807
BsmI	GAATGC	1 cut(s)	694
BsnI	GGCC	6 cut(s)	65, 86, 338, 827, 1095, 1229
BsoBI	CYCGRG	1 cut(s)	1811
Bsp1286I	GDGCHC	1 cut(s)	901
Bsp1407I	TGTACA	1 cut(s)	98
Bsp143I	GATC	8 cut(s)	67, 259, 1012, 1038, 1200, 1494, 1579, 1705
BspACI	CCGC	6 cut(s)	57, 87, 111, 187, 834, 1574
BspANI	GGCC	6 cut(s)	65, 86, 338, 827, 1095, 1229
BspCNI	CTCAG	7 cut(s)	306, 307, 598, 1170, 1210, 1399, 1738
BspHI	TCATGA	2 cut(s)	1141, 1237
BspLI	GGNNCC	5 cut(s)	217, 320, 547, 655, 1404
BspMAI	CTGCAG	1 cut(s)	1663
BspPI	GGATC	4 cut(s)	62, 1020, 1502, 1574
BspT107I	GGYRCC	2 cut(s)	215, 318
BsrBI	CCGCTC	2 cut(s)	59, 89
BsrDI	GCAATG	1 cut(s)	1377
BsrFI	RCCGGY	1 cut(s)	1382
BsrGI	TGTACA	1 cut(s)	98
BsrI	ACTGG	3 cut(s)	17, 1096, 1583
BssAI	RCCGGY	1 cut(s)	1382
BssECI	CCNNGG	1 cut(s)	159
BssMI	GATC	8 cut(s)	67, 259, 1012, 1038, 1200, 1494, 1579, 1705
BssNAI	GTATAC	1 cut(s)	235
BssSI	CACGAG	4 cut(s)	72, 342, 427, 1419
Bst1107I	GTATAC	1 cut(s)	235
Bst2BI	CACGAG	4 cut(s)	72, 342, 427, 1419
Bst2UI	CCWGG	2 cut(s)	152, 161
Bst4CI	ACNGT	8 cut(s)	132, 232, 469, 571, 640, 742, 773, 1454
Bst6I	CTCTTC	1 cut(s)	866
BstAPI	GCANNNNNTGC	1 cut(s)	285
BstAUI	TGTACA	1 cut(s)	98
BstC8I	GCNNGC	3 cut(s)	323, 694, 825
BstDEI	CTNAG	7 cut(s)	294, 314, 585, 1178, 1218, 1407, 1725
BstENI	CCTNNNNNAGG	1 cut(s)	666
BstF5I	GGATG	4 cut(s)	12, 754, 1085, 1303
BstH2I	RGCGCY	1 cut(s)	420
BstHHI	GCGC	1 cut(s)	419
BstKTI	GATC	8 cut(s)	70, 262, 1015, 1041, 1203, 1497, 1582, 1708
BstMAI	GTCTC	2 cut(s)	121, 1821
BstMBI	GATC	8 cut(s)	67, 259, 1012, 1038, 1200, 1494, 1579, 1705
BstMWI	GCNNNNNNNGC	8 cut(s)	83, 119, 285, 812, 833, 1092, 1101, 1735
BstNI	CCWGG	2 cut(s)	152, 161
BstSCI	CCNGG	2 cut(s)	150, 159
BstSFI	CTRYAG	2 cut(s)	1529, 1659
BstV1I	GCAGC	6 cut(s)	167, 187, 505, 793, 1095, 1653
BstV2I	GAAGAC	1 cut(s)	429
BstX2I	RGATCY	1 cut(s)	259
BstXI	CCANNNNNNTGG	2 cut(s)	151, 1182
BstYI	RGATCY	1 cut(s)	259
BstZ17I	GTATAC	1 cut(s)	235
BsuRI	GGCC	6 cut(s)	65, 86, 338, 827, 1095, 1229
BtsCI	GGATG	4 cut(s)	12, 754, 1085, 1303
BtsI	GCAGTG	1 cut(s)	1371
BtsIMutI	CAGTG	6 cut(s)	302, 593, 1048, 1175, 1371, 1701
Cac8I	GCNNGC	3 cut(s)	323, 694, 825
CaiI	CAGNNNCTG	1 cut(s)	313
CciI	TCATGA	2 cut(s)	1141, 1237
CfoI	GCGC	1 cut(s)	419
Cfr10I	RCCGGY	1 cut(s)	1382
Cfr13I	GGNCC	3 cut(s)	546, 654, 1094
Csp6I	GTAC	4 cut(s)	99, 196, 382, 1386
CviAII	CATG	4 cut(s)	253, 1142, 1238, 1309
CviQI	GTAC	4 cut(s)	99, 196, 382, 1386
DdeI	CTNAG	7 cut(s)	294, 314, 585, 1178, 1218, 1407, 1725
DpnI	GATC	8 cut(s)	69, 261, 1014, 1040, 1202, 1496, 1581, 1707
DpnII	GATC	8 cut(s)	67, 259, 1012, 1038, 1200, 1494, 1579, 1705
DraI	TTTAAA	1 cut(s)	475
DrdI	GACNNNNNNGTC	1 cut(s)	431
DseDI	GACNNNNNNGTC	1 cut(s)	431
EaeI	YGGCCR	2 cut(s)	63, 825
Eam1104I	CTCTTC	1 cut(s)	866
EarI	CTCTTC	1 cut(s)	866
EciI	GGCGGA	1 cut(s)	823
Eco47I	GGWCC	2 cut(s)	546, 654
Eco47III	AGCGCT	1 cut(s)	418
Eco57I	CTGAAG	2 cut(s)	441, 1371
Eco88I	CYCGRG	1 cut(s)	1811
EcoNI	CCTNNNNNAGG	1 cut(s)	666
EcoO109I	RGGNCCY	1 cut(s)	654
EcoRII	CCWGG	2 cut(s)	150, 159
EcoT22I	ATGCAT	1 cut(s)	487
FaeI	CATG	4 cut(s)	256, 1145, 1241, 1312
FaqI	GGGAC	4 cut(s)	907, 1225, 1556, 1807
FatI	CATG	4 cut(s)	252, 1141, 1237, 1308
FauI	CCCGC	1 cut(s)	194
FauNDI	CATATG	1 cut(s)	940
FbaI	TGATCA	2 cut(s)	1038, 1705
FblI	GTMKAC	1 cut(s)	234
Fnu4HI	GCNGC	7 cut(s)	87, 156, 201, 519, 807, 1084, 1667
FokI	GGATG	3 cut(s)	761, 1092, 1310
Fsp4HI	GCNGC	7 cut(s)	87, 156, 201, 519, 807, 1084, 1667
FspBI	CTAG	5 cut(s)	645, 893, 1338, 1589, 1866
GlaI	GCGC	1 cut(s)	418
GluI	GCNGC	7 cut(s)	87, 156, 201, 519, 807, 1084, 1667
GsuI	CTGGAG	1 cut(s)	887
HaeII	RGCGCY	1 cut(s)	420
HaeIII	GGCC	6 cut(s)	65, 86, 338, 827, 1095, 1229
HapII	CCGG	3 cut(s)	219, 379, 1383
HhaI	GCGC	1 cut(s)	419
Hin1II	CATG	4 cut(s)	256, 1145, 1241, 1312
Hin6I	GCGC	1 cut(s)	417
HinP1I	GCGC	1 cut(s)	417
HincII	GTYRAC	3 cut(s)	211, 445, 1431
HindII	GTYRAC	3 cut(s)	211, 445, 1431
HinfI	GANTC	9 cut(s)	310, 460, 553, 648, 1047, 1244, 1332, 1711, 1814
HpaI	GTTAAC	1 cut(s)	445
HpaII	CCGG	3 cut(s)	219, 379, 1383
HphI	GGTGA	2 cut(s)	508, 754
Hpy166II	GTNNAC	6 cut(s)	211, 235, 445, 916, 1431, 1450
Hpy188I	TCNGA	7 cut(s)	93, 315, 333, 408, 666, 958, 1710
Hpy8I	GTNNAC	6 cut(s)	211, 235, 445, 916, 1431, 1450
Hpy99I	CGWCG	4 cut(s)	209, 368, 389, 777
HpyAV	CCTTC	8 cut(s)	145, 328, 668, 938, 1115, 1148, 1432, 1797
HpyCH4III	ACNGT	8 cut(s)	132, 232, 469, 571, 640, 742, 773, 1454
HpyCH4IV	ACGT	1 cut(s)	1446
HpyF10VI	GCNNNNNNNGC	8 cut(s)	83, 119, 285, 812, 833, 1092, 1101, 1735
HpyF3I	CTNAG	7 cut(s)	294, 314, 585, 1178, 1218, 1407, 1725
HpySE526I	ACGT	1 cut(s)	1446
Hsp92II	CATG	4 cut(s)	256, 1145, 1241, 1312
HspAI	GCGC	1 cut(s)	417
Ksp22I	TGATCA	2 cut(s)	1038, 1705
KspAI	GTTAAC	1 cut(s)	445
Kzo9I	GATC	8 cut(s)	67, 259, 1012, 1038, 1200, 1494, 1579, 1705
LmnI	GCTCC	1 cut(s)	1408
Lsp1109I	GCAGC	6 cut(s)	167, 187, 505, 793, 1095, 1653
LweI	GCATC	4 cut(s)	21, 1070, 1517, 1625
MaeI	CTAG	5 cut(s)	645, 893, 1338, 1589, 1866
MaeII	ACGT	1 cut(s)	1446
MaeIII	GTNAC	8 cut(s)	45, 550, 565, 632, 640, 698, 742, 1414
MalI	GATC	8 cut(s)	69, 261, 1014, 1040, 1202, 1496, 1581, 1707
MbiI	CCGCTC	2 cut(s)	59, 89
MboI	GATC	8 cut(s)	67, 259, 1012, 1038, 1200, 1494, 1579, 1705
MflI	RGATCY	1 cut(s)	259
MhlI	GDGCHC	1 cut(s)	901
MlsI	TGGCCA	1 cut(s)	827
MluNI	TGGCCA	1 cut(s)	827
MlyI	GAGTC	3 cut(s)	547, 1326, 1823
MmeI	TCCRAC	1 cut(s)	1615
Mox20I	TGGCCA	1 cut(s)	827
Mph1103I	ATGCAT	1 cut(s)	487
MroXI	GAANNNNTTC	1 cut(s)	1104
MscI	TGGCCA	1 cut(s)	827
MseI	TTAA	4 cut(s)	402, 444, 474, 1280
MslI	CAYNNNNRTG	1 cut(s)	1180
Msp20I	TGGCCA	1 cut(s)	827
MspA1I	CMGCKG	1 cut(s)	1217
MspI	CCGG	3 cut(s)	219, 379, 1383
MspR9I	CCNGG	2 cut(s)	152, 161
Mva1269I	GAATGC	1 cut(s)	694
MvaI	CCWGG	2 cut(s)	152, 161
MwoI	GCNNNNNNNGC	8 cut(s)	83, 119, 285, 812, 833, 1092, 1101, 1735
NdeI	CATATG	1 cut(s)	940
NdeII	GATC	8 cut(s)	67, 259, 1012, 1038, 1200, 1494, 1579, 1705
NlaIII	CATG	4 cut(s)	256, 1145, 1241, 1312
NlaIV	GGNNCC	5 cut(s)	217, 320, 547, 655, 1404
NmeAIII	GCCGAG	1 cut(s)	535
NmuCI	GTSAC	5 cut(s)	550, 632, 640, 742, 1414
NsiI	ATGCAT	1 cut(s)	487
OliI	CACNNNNGTG	1 cut(s)	1180
PagI	TCATGA	2 cut(s)	1141, 1237
PcsI	WCGNNNNNNNCGW	2 cut(s)	204, 243
PctI	GAATGC	1 cut(s)	694
PdmI	GAANNNNTTC	1 cut(s)	1104
PfeI	GAWTC	6 cut(s)	310, 460, 648, 1047, 1244, 1711
PflFI	GACNNNGTC	2 cut(s)	638, 771
PkrI	GCNGC	7 cut(s)	88, 157, 202, 520, 808, 1085, 1668
PleI	GAGTC	3 cut(s)	547, 1326, 1822
PpsI	GAGTC	3 cut(s)	547, 1326, 1822
PpuMI	RGGWCCY	1 cut(s)	654
PsiI	TTATAA	2 cut(s)	729, 797
Psp5II	RGGWCCY	1 cut(s)	654
Psp6I	CCWGG	2 cut(s)	150, 159
PspGI	CCWGG	2 cut(s)	150, 159
PspN4I	GGNNCC	5 cut(s)	217, 320, 547, 655, 1404
PspPI	GGNCC	3 cut(s)	546, 654, 1094
PspPPI	RGGWCCY	1 cut(s)	654
PstI	CTGCAG	1 cut(s)	1663
PstNI	CAGNNNCTG	1 cut(s)	313
PsuI	RGATCY	1 cut(s)	259
PsyI	GACNNNGTC	2 cut(s)	638, 771
PvuII	CAGCTG	1 cut(s)	1217
RsaI	GTAC	4 cut(s)	100, 197, 383, 1387
RsaNI	GTAC	4 cut(s)	99, 196, 382, 1386
RseI	CAYNNNNRTG	1 cut(s)	1180
SaqAI	TTAA	4 cut(s)	402, 444, 474, 1280
SatI	GCNGC	7 cut(s)	87, 156, 201, 519, 807, 1084, 1667
Sau3AI	GATC	8 cut(s)	67, 259, 1012, 1038, 1200, 1494, 1579, 1705
Sau96I	GGNCC	3 cut(s)	546, 654, 1094
SchI	GAGTC	3 cut(s)	547, 1326, 1823
ScrFI	CCNGG	2 cut(s)	152, 161
SduI	GDGCHC	1 cut(s)	901
SfaNI	GCATC	4 cut(s)	21, 1070, 1517, 1625
SfcI	CTRYAG	2 cut(s)	1529, 1659
SinI	GGWCC	2 cut(s)	546, 654
SmiMI	CAYNNNNRTG	1 cut(s)	1180
SmlI	CTYRAG	2 cut(s)	703, 1169
SmoI	CTYRAG	2 cut(s)	703, 1169
SsiI	CCGC	6 cut(s)	57, 87, 111, 187, 834, 1574
SspI	AATATT	1 cut(s)	1783
SspMI	CTAG	5 cut(s)	645, 893, 1338, 1589, 1866
StyD4I	CCNGG	2 cut(s)	150, 159
TaaI	ACNGT	8 cut(s)	132, 232, 469, 571, 640, 742, 773, 1454
TaiI	ACGT	1 cut(s)	1449
TatI	WGTACW	1 cut(s)	98
TauI	GCSGC	1 cut(s)	89
TfiI	GAWTC	6 cut(s)	310, 460, 648, 1047, 1244, 1711
Tru1I	TTAA	4 cut(s)	402, 444, 474, 1280
Tru9I	TTAA	4 cut(s)	402, 444, 474, 1280
TscAI	CASTG	6 cut(s)	302, 593, 1048, 1182, 1378, 1708
TseFI	GTSAC	5 cut(s)	550, 632, 640, 742, 1414
TseI	GCWGC	6 cut(s)	155, 200, 518, 806, 1083, 1666
Tsp45I	GTSAC	5 cut(s)	550, 632, 640, 742, 1414
TspGWI	ACGGA	3 cut(s)	538, 761, 1448
TspRI	CASTG	6 cut(s)	302, 593, 1048, 1182, 1378, 1708
Tth111I	GACNNNGTC	2 cut(s)	638, 771
VpaK11BI	GGWCC	2 cut(s)	546, 654
XagI	CCTNNNNNAGG	1 cut(s)	666
XapI	RAATTY	3 cut(s)	502, 1513, 1719
XbaI	TCTAGA	1 cut(s)	1588
XmiI	GTMKAC	1 cut(s)	234
XmnI	GAANNNNTTC	1 cut(s)	1104
XspI	CTAG	5 cut(s)	645, 893, 1338, 1589, 1866
Zsp2I	ATGCAT	1 cut(s)	487

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.