Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0009108

Physical Location & Seq

Genomic Coordinates Forward (+)

8368110 .. 8371323

Gene Structure 3214 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1115400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1461 bp

ATGGCACCCATCAAGGGTATACTTTCTCTGCAGAGAGCAGCTTTAGCTCGTCATCATGGTGCGAAGTGGGGCCTAGGGTTTAGATCATTTAGCACTCAGGGTGCAGCACCCTCTACTACTCCTCAACCTCCACCACCTCCTCCACCTCCTGAAAAAACCCATTTTGGTGGTTTGAAAGATGAAGACCGAATTTTCACCAACTTATATGGGTTGCACGACCCATTTCTCAAGGGTGCCATGAAACGAGGTGACTGGTACCGAACAAAAGATCTAGTTATTAAAGGTGCTGATTGGATTGTCAATGAAATGAAGAAGTCTGGCCTCCGTGGACGTGGTGGTGCTGGTTTCCCATCTGGCCTCAAATGGTCTTTTATGCCAAAAGTATCTGATGGCCGTCCATCCTATCTTGTCGTCAATGCTGATGAAAGTGAACCTGGAACCTGTAAGGACAGGGAAATTATGCGCCATGACCCACACAAACTTTTAGAGGGTTGCTTGATTGCTGGTGTTGGGATGAGGGCTAGTGCTGCTTACATCTATATAAGAGGTGAATATGTGAATGAACGGTTAAACCTTGTAAAGGCCAGAAATGAAGCCTATGCAGCTGGATTACTGGGAAAAAATGCATGTGGATCTGGTTATGATTTTGATGTTCACATCCACTTTGGTGCTGGGGCCTATATTTGTGGTGAAGAAACAGCTCTTCTAGAGAGCCTTGAAGGGAAACAGGGTAAGCCAAGATTGAAGCCTCCCTTCCCTGCTAATGCAGGATTATATGGCTGCCCCACCACCGTTACAAATGTGGAAACGGTGGCTGTTTCTCCTACCATTTTGAGGCGTGGACCAGAGTGGTTTGCCAGTTTTGGGAGAAAGAACAATTCAGGGACAAAATTGTTCTGTATTTCAGGACATGTGAACAAGCCTTGCACCGTTGAGGAGGAGATGAGCATACCCTTGAAAGAGTTATTAGAGAGGCACTGTGGAGGTGTGAGGGGTGGATGGGACAATTTACTTGCAGTAATTCCAGGTGGTTCATCCGTTCCATTGCTTACCAAGGACATATGTAATGATGTATTGATGGATTTTGATGCACTGAAAGCTGTCCAGTCAGGATTGGGAACTGCAGCTGTTATTGTGATGGATAAATCGACTGACATTGTCGATGCAATTGCAAGGCTTTCTTACTTCTACAAGCATGAGAGCTGTGGGCAGTGCACACCATGCAGGGAGGGGACAGGATGGCTTTGGATGATCATGGAAAGAATGAAAGTAGGGAATGCAAAGCTGGAAGAGATTGACATGCTTCAGGAGGTGACCAAGCAGATTGAGGGGCACACAATCTGTGCTTTGGGTGATGCTGCTGCTTGGCCTGTGCAAGGTTTAATAAGGCATTTCAGGCCAGAGCTTGAGAGGAGGATCAGGGAGCGTGCTGAGAAGGAATTGCTGGAGGCAGCTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005746 GO:0005747 GO:0016020 GO:0019866 GO:0030964 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045271 GO:0070469 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016

Pfam Domains

Protein Families

Complex1_51K NADH_4Fe-4S SLBB

Protein Analysis

487

Amino Acids

53.18

Weight (kDa)

8.45

Isoelectric Point (pI)

38.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Complex1_51K	PF01512	103 - 272	3.7e-44	Nuo51 FMN-binding domain
SLBB_2	PF22461	297 - 379	7.3e-18	SLBB domain
SLBB	PF10531	299 - 347	8.9e-06	SLBB domain
NADH_4Fe-4S	PF10589	388 - 471	2.6e-32	NADH-ubiquinone oxidoreductase-F iron-sulfur binding region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009108)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08530
fragaria_vesca	FvH4_4g24110 FvH4_4g24110 FvH4_4g24110
malus_domestica	MD13G1115400.v1.1 MD16G1115300.v1.1
prunus_persica	Prupe.1G231900_v2.0.a1 Prupe.1G231900_v2.0.a1
pyrus_communis	pycom13g09990 pycom16g09780
rosa_chinensis	RchiOBHm_Chr4g0430891
rosa_laevigata	RLG00000006941
rosa_multiflora	Rmu_sc0006263.1_g000009
rosa_roxburghii	Rroxscaffold_5G00372580
rosa_rugosa	Rorug04G0247100 Rorug04G0247200
rosa_samantha	Rh4AG302600 Rh4AG303500 Rh4BG310700 Rh4CG327100 Rh4DG306900
rosa_wichuraiana	Rw4G026300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	255
AccB1I	GGYRCC	3 cut(s)	4, 233, 255
AccI	GTMKAC	1 cut(s)	19
AclWI	GGATC	2 cut(s)	640, 1424
AcoI	YGGCCR	1 cut(s)	391
AcsI	RAATTY	1 cut(s)	189
AcuI	CTGAAG	1 cut(s)	1289
AfaI	GTAC	1 cut(s)	257
AfiI	CCNNNNNNNGG	5 cut(s)	14, 580, 834, 864, 1376
AflIII	ACRYGT	1 cut(s)	910
AgsI	TTSAA	4 cut(s)	175, 719, 745, 958
AjiI	CACGTC	1 cut(s)	332
AjnI	CCWGG	2 cut(s)	433, 1024
AleI	CACNNNNGTG	1 cut(s)	666
Alw21I	GWGCWC	1 cut(s)	1217
Alw44I	GTGCAC	1 cut(s)	1213
AlwI	GGATC	2 cut(s)	640, 1424
AoxI	GGCC	8 cut(s)	70, 319, 355, 391, 582, 675, 1367, 1397
ApaLI	GTGCAC	1 cut(s)	1213
ApoI	RAATTY	1 cut(s)	189
Asp718I	GGTACC	1 cut(s)	255
AspA2I	CCTAGG	1 cut(s)	73
AspLEI	GCGC	1 cut(s)	465
AspS9I	GGNCC	3 cut(s)	70, 675, 842
AsuHPI	GGTGA	6 cut(s)	187, 260, 560, 701, 1324, 1364
AvaII	GGWCC	1 cut(s)	842
AvrII	CCTAGG	1 cut(s)	73
BaeGI	GKGCMC	2 cut(s)	1217, 1335
BanI	GGYRCC	3 cut(s)	4, 233, 255
BbsI	GAAGAC	1 cut(s)	189
Bbv12I	GWGCWC	1 cut(s)	1217
BbvI	GCAGC	9 cut(s)	50, 116, 514, 614, 767, 1136, 1345, 1348, 1441
BccI	CCATC	8 cut(s)	17, 358, 383, 406, 993, 1072, 1132, 1233
BceAI	ACGGC	1 cut(s)	378
BcgI	CGANNNNNNTGC	2 cut(s)	1151, 1185
BciT130I	CCWGG	2 cut(s)	435, 1026
BclI	TGATCA	1 cut(s)	1251
BfaI	CTAG	4 cut(s)	74, 272, 522, 707
BfmI	CTRYAG	2 cut(s)	29, 1122
BglII	AGATCT	1 cut(s)	268
BlnI	CCTAGG	1 cut(s)	73
Bme1390I	CCNGG	2 cut(s)	435, 1026
Bme18I	GGWCC	1 cut(s)	842
BmgBI	CACGTC	1 cut(s)	332
BmgT120I	GGNCC	3 cut(s)	70, 675, 842
BmiI	GGNNCC	6 cut(s)	6, 71, 235, 257, 439, 676
BmrFI	CCNGG	2 cut(s)	435, 1026
BmrI	ACTGGG	1 cut(s)	623
BmsI	GCATC	3 cut(s)	1078, 1153, 1345
BmuI	ACTGGG	1 cut(s)	623
BpiI	GAAGAC	1 cut(s)	189
BpuEI	CTTGAG	2 cut(s)	212, 1427
BsaJI	CCNNGG	3 cut(s)	73, 325, 1053
Bsc4I	CCNNNNNNNGG	5 cut(s)	14, 580, 834, 864, 1376
Bse1I	ACTGG	4 cut(s)	257, 618, 858, 1105
Bse3DI	GCAATG	1 cut(s)	1043
BseBI	CCWGG	2 cut(s)	435, 1026
BseDI	CCNNGG	3 cut(s)	73, 325, 1053
BseGI	GGATG	7 cut(s)	398, 519, 657, 1004, 1034, 1244, 1254
BseLI	CCNNNNNNNGG	5 cut(s)	14, 580, 834, 864, 1376
BseMI	GCAATG	1 cut(s)	1043
BseMII	CTCAG	2 cut(s)	110, 1422
BseNI	ACTGG	4 cut(s)	257, 618, 858, 1105
BseRI	GAGGAG	5 cut(s)	111, 129, 950, 953, 1426
BseSI	GKGCMC	2 cut(s)	1217, 1335
BseXI	GCAGC	9 cut(s)	50, 116, 514, 614, 767, 1136, 1345, 1348, 1441
BseYI	CCCAGC	1 cut(s)	671
BsgI	GTGCAG	1 cut(s)	123
BshFI	GGCC	8 cut(s)	72, 321, 357, 393, 584, 677, 1369, 1399
BshNI	GGYRCC	3 cut(s)	4, 233, 255
BsiHKAI	GWGCWC	1 cut(s)	1217
BslFI	GGGAC	3 cut(s)	898, 1016, 1246
BslI	CCNNNNNNNGG	5 cut(s)	14, 580, 834, 864, 1376
BsmFI	GGGAC	3 cut(s)	898, 1016, 1246
BsmI	GAATGC	1 cut(s)	1282
BsnI	GGCC	8 cut(s)	72, 321, 357, 393, 584, 677, 1369, 1399
Bsp1286I	GDGCHC	2 cut(s)	1217, 1335
Bsp143I	GATC	5 cut(s)	83, 268, 632, 1251, 1416
BspANI	GGCC	8 cut(s)	72, 321, 357, 393, 584, 677, 1369, 1399
BspCNI	CTCAG	2 cut(s)	109, 1423
BspLI	GGNNCC	6 cut(s)	6, 71, 235, 257, 439, 676
BspMAI	CTGCAG	2 cut(s)	33, 1126
BspPI	GGATC	2 cut(s)	640, 1424
BspQI	GCTCTTC	1 cut(s)	708
BspT107I	GGYRCC	3 cut(s)	4, 233, 255
BsrDI	GCAATG	1 cut(s)	1043
BsrI	ACTGG	4 cut(s)	257, 618, 858, 1105
BssECI	CCNNGG	3 cut(s)	73, 325, 1053
BssMI	GATC	5 cut(s)	83, 268, 632, 1251, 1416
BssNAI	GTATAC	1 cut(s)	20
BssT1I	CCWWGG	2 cut(s)	73, 1053
Bst1107I	GTATAC	1 cut(s)	20
Bst2UI	CCWGG	2 cut(s)	435, 1026
Bst4CI	ACNGT	5 cut(s)	567, 793, 811, 931, 980
Bst6I	CTCTTC	2 cut(s)	708, 1284
BstAPI	GCANNNNNTGC	1 cut(s)	1221
BstC8I	GCNNGC	1 cut(s)	1428
BstDEI	CTNAG	2 cut(s)	96, 1431
BstDSI	CCRYGG	1 cut(s)	325
BstEII	GGTNACC	1 cut(s)	1312
BstENI	CCTNNNNNAGG	2 cut(s)	578, 1374
BstF5I	GGATG	7 cut(s)	398, 519, 657, 1004, 1034, 1244, 1254
BstHHI	GCGC	1 cut(s)	465
BstKTI	GATC	5 cut(s)	86, 271, 635, 1254, 1419
BstMBI	GATC	5 cut(s)	83, 268, 632, 1251, 1416
BstMWI	GCNNNNNNNGC	6 cut(s)	44, 527, 602, 1097, 1221, 1396
BstNI	CCWGG	2 cut(s)	435, 1026
BstNSI	RCATGY	3 cut(s)	630, 914, 1303
BstPI	GGTNACC	1 cut(s)	1312
BstSCI	CCNGG	2 cut(s)	433, 1024
BstSFI	CTRYAG	2 cut(s)	29, 1122
BstSLI	GKGCMC	2 cut(s)	1217, 1335
BstV1I	GCAGC	9 cut(s)	50, 116, 514, 614, 767, 1136, 1345, 1348, 1441
BstV2I	GAAGAC	1 cut(s)	189
BstX2I	RGATCY	2 cut(s)	268, 632
BstXI	CCANNNNNNTGG	1 cut(s)	167
BstYI	RGATCY	2 cut(s)	268, 632
BstZ17I	GTATAC	1 cut(s)	20
BsuRI	GGCC	8 cut(s)	72, 321, 357, 393, 584, 677, 1369, 1399
BtgI	CCRYGG	1 cut(s)	325
BtrI	CACGTC	1 cut(s)	332
BtsCI	GGATG	7 cut(s)	398, 519, 657, 1004, 1034, 1244, 1254
BtsI	GCAGTG	1 cut(s)	1217
BtsIMutI	CAGTG	3 cut(s)	976, 1091, 1217
Cac8I	GCNNGC	1 cut(s)	1428
CfoI	GCGC	1 cut(s)	465
Cfr13I	GGNCC	3 cut(s)	70, 675, 842
Csp6I	GTAC	1 cut(s)	256
CviAII	CATG	9 cut(s)	56, 238, 467, 627, 911, 1196, 1221, 1255, 1300
CviQI	GTAC	1 cut(s)	256
DdeI	CTNAG	2 cut(s)	96, 1431
DpnI	GATC	5 cut(s)	85, 270, 634, 1253, 1418
DpnII	GATC	5 cut(s)	83, 268, 632, 1251, 1416
EaeI	YGGCCR	1 cut(s)	391
Eam1104I	CTCTTC	2 cut(s)	708, 1284
EarI	CTCTTC	2 cut(s)	708, 1284
Eco130I	CCWWGG	2 cut(s)	73, 1053
Eco47I	GGWCC	1 cut(s)	842
Eco57I	CTGAAG	1 cut(s)	1289
Eco91I	GGTNACC	1 cut(s)	1312
EcoNI	CCTNNNNNAGG	2 cut(s)	578, 1374
EcoO109I	RGGNCCY	2 cut(s)	70, 675
EcoO65I	GGTNACC	1 cut(s)	1312
EcoRII	CCWGG	2 cut(s)	433, 1024
EcoT14I	CCWWGG	2 cut(s)	73, 1053
EcoT22I	ATGCAT	1 cut(s)	628
ErhI	CCWWGG	2 cut(s)	73, 1053
FaeI	CATG	9 cut(s)	59, 241, 470, 630, 914, 1199, 1224, 1258, 1303
FalI	AAGNNNNNCTT	4 cut(s)	737, 769, 1165, 1197
FaqI	GGGAC	3 cut(s)	898, 1016, 1246
FatI	CATG	9 cut(s)	55, 237, 466, 626, 910, 1195, 1220, 1254, 1299
FauNDI	CATATG	1 cut(s)	1061
FbaI	TGATCA	1 cut(s)	1251
FblI	GTMKAC	1 cut(s)	19
FokI	GGATG	7 cut(s)	385, 526, 644, 1011, 1021, 1251, 1261
FspBI	CTAG	4 cut(s)	74, 272, 522, 707
GlaI	GCGC	1 cut(s)	464
GsaI	CCCAGC	1 cut(s)	675
HaeIII	GGCC	8 cut(s)	72, 321, 357, 393, 584, 677, 1369, 1399
HhaI	GCGC	1 cut(s)	465
Hin1II	CATG	9 cut(s)	59, 241, 470, 630, 914, 1199, 1224, 1258, 1303
Hin6I	GCGC	1 cut(s)	463
HinP1I	GCGC	1 cut(s)	463
HphI	GGTGA	6 cut(s)	187, 260, 560, 701, 1324, 1364
Hpy166II	GTNNAC	7 cut(s)	20, 329, 431, 655, 842, 916, 1215
Hpy188I	TCNGA	1 cut(s)	388
Hpy188III	TCNNGA	5 cut(s)	149, 707, 906, 1110, 1307
Hpy8I	GTNNAC	7 cut(s)	20, 329, 431, 655, 842, 916, 1215
HpyAV	CCTTC	3 cut(s)	713, 763, 1429
HpyCH4III	ACNGT	5 cut(s)	567, 793, 811, 931, 980
HpyCH4IV	ACGT	1 cut(s)	331
HpyF10VI	GCNNNNNNNGC	6 cut(s)	44, 527, 602, 1097, 1221, 1396
HpyF3I	CTNAG	2 cut(s)	96, 1431
HpySE526I	ACGT	1 cut(s)	331
Hsp92II	CATG	9 cut(s)	59, 241, 470, 630, 914, 1199, 1224, 1258, 1303
HspAI	GCGC	1 cut(s)	463
KpnI	GGTACC	1 cut(s)	259
Ksp22I	TGATCA	1 cut(s)	1251
Kzo9I	GATC	5 cut(s)	83, 268, 632, 1251, 1416
LguI	GCTCTTC	1 cut(s)	708
LmnI	GCTCC	1 cut(s)	1423
Lsp1109I	GCAGC	9 cut(s)	50, 116, 514, 614, 767, 1136, 1345, 1348, 1441
LweI	GCATC	3 cut(s)	1078, 1153, 1345
MaeI	CTAG	4 cut(s)	74, 272, 522, 707
MaeII	ACGT	1 cut(s)	331
MaeIII	GTNAC	3 cut(s)	248, 793, 1312
MalI	GATC	5 cut(s)	85, 270, 634, 1253, 1418
MboI	GATC	5 cut(s)	83, 268, 632, 1251, 1416
MboII	GAAGA	5 cut(s)	194, 322, 695, 704, 1301
MfeI	CAATTG	1 cut(s)	1167
MflI	RGATCY	2 cut(s)	268, 632
MhlI	GDGCHC	2 cut(s)	1217, 1335
MluCI	AATT	8 cut(s)	189, 456, 877, 890, 1006, 1020, 1167, 1439
Mph1103I	ATGCAT	1 cut(s)	628
MseI	TTAA	4 cut(s)	279, 569, 1382, 1459
MslI	CAYNNNNRTG	3 cut(s)	57, 165, 666
MspA1I	CMGCKG	3 cut(s)	605, 1127, 1454
MspR9I	CCNGG	2 cut(s)	435, 1026
MunI	CAATTG	1 cut(s)	1167
Mva1269I	GAATGC	1 cut(s)	1282
MvaI	CCWGG	2 cut(s)	435, 1026
MwoI	GCNNNNNNNGC	6 cut(s)	44, 527, 602, 1097, 1221, 1396
NdeI	CATATG	1 cut(s)	1061
NdeII	GATC	5 cut(s)	83, 268, 632, 1251, 1416
NlaIII	CATG	9 cut(s)	59, 241, 470, 630, 914, 1199, 1224, 1258, 1303
NlaIV	GGNNCC	6 cut(s)	6, 71, 235, 257, 439, 676
NmuCI	GTSAC	2 cut(s)	248, 1312
NsiI	ATGCAT	1 cut(s)	628
NspI	RCATGY	3 cut(s)	630, 914, 1303
OliI	CACNNNNGTG	1 cut(s)	666
PciI	ACATGT	1 cut(s)	910
PciSI	GCTCTTC	1 cut(s)	708
PctI	GAATGC	1 cut(s)	1282
PflFI	GACNNNGTC	1 cut(s)	1157
PscI	ACATGT	1 cut(s)	910
Psp6I	CCWGG	2 cut(s)	433, 1024
PspEI	GGTNACC	1 cut(s)	1312
PspFI	CCCAGC	1 cut(s)	671
PspGI	CCWGG	2 cut(s)	433, 1024
PspN4I	GGNNCC	6 cut(s)	6, 71, 235, 257, 439, 676
PspPI	GGNCC	3 cut(s)	70, 675, 842
PstI	CTGCAG	2 cut(s)	33, 1126
PsuI	RGATCY	2 cut(s)	268, 632
PsyI	GACNNNGTC	1 cut(s)	1157
PvuII	CAGCTG	3 cut(s)	605, 1127, 1454
RsaI	GTAC	1 cut(s)	257
RsaNI	GTAC	1 cut(s)	256
RseI	CAYNNNNRTG	3 cut(s)	57, 165, 666
SapI	GCTCTTC	1 cut(s)	708
SaqAI	TTAA	4 cut(s)	279, 569, 1382, 1459
Sau3AI	GATC	5 cut(s)	83, 268, 632, 1251, 1416
Sau96I	GGNCC	3 cut(s)	70, 675, 842
ScrFI	CCNGG	2 cut(s)	435, 1026
SduI	GDGCHC	2 cut(s)	1217, 1335
SfaNI	GCATC	3 cut(s)	1078, 1153, 1345
SfcI	CTRYAG	2 cut(s)	29, 1122
SinI	GGWCC	1 cut(s)	842
SmiMI	CAYNNNNRTG	3 cut(s)	57, 165, 666
SmlI	CTYRAG	2 cut(s)	227, 1406
SmoI	CTYRAG	2 cut(s)	227, 1406
Sse9I	AATT	8 cut(s)	189, 456, 877, 890, 1006, 1020, 1167, 1439
SspMI	CTAG	4 cut(s)	74, 272, 522, 707
StyD4I	CCNGG	2 cut(s)	433, 1024
StyI	CCWWGG	2 cut(s)	73, 1053
TaaI	ACNGT	5 cut(s)	567, 793, 811, 931, 980
TaiI	ACGT	1 cut(s)	334
TaqI	TCGA	2 cut(s)	1148, 1161
TaqII	GACCGA	1 cut(s)	201
TasI	AATT	8 cut(s)	189, 456, 877, 890, 1006, 1020, 1167, 1439
Tru1I	TTAA	4 cut(s)	279, 569, 1382, 1459
Tru9I	TTAA	4 cut(s)	279, 569, 1382, 1459
TscAI	CASTG	3 cut(s)	983, 1098, 1217
TseFI	GTSAC	2 cut(s)	248, 1312
Tsp45I	GTSAC	2 cut(s)	248, 1312
TspDTI	ATGAA	9 cut(s)	195, 254, 318, 323, 438, 576, 606, 1023, 1280
TspGWI	ACGGA	2 cut(s)	314, 1027
TspRI	CASTG	3 cut(s)	983, 1098, 1217
Tth111I	GACNNNGTC	1 cut(s)	1157
VneI	GTGCAC	1 cut(s)	1213
VpaK11BI	GGWCC	1 cut(s)	842
XagI	CCTNNNNNAGG	2 cut(s)	578, 1374
XapI	RAATTY	1 cut(s)	189
XbaI	TCTAGA	1 cut(s)	706
XceI	RCATGY	3 cut(s)	630, 914, 1303
XcmI	CCANNNNNNNNNTGG	1 cut(s)	668
XmaJI	CCTAGG	1 cut(s)	73
XmiI	GTMKAC	1 cut(s)	19
XspI	CTAG	4 cut(s)	74, 272, 522, 707
Zsp2I	ATGCAT	1 cut(s)	628

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.