MD13G1120200.v1.1

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0018774
Physical Location & Seq
Forward (+)
8835673 .. 8835939
267 bp
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UTR
Exon/CDS
Intron
MD13G1120200.v1.1.491

Sequence Viewer

Length: 267 bp
ATGGATGTGAAGGAGAATAAGGAGGCTCTGTTTTTGCGAATGGACATGCCAAGATTGGACAAGCAAGACGTGAAGATTTCGGTGGAGCAGAACACCCTGGTAGTGAGAGGGGAGGGGAAGGACTCGGAGGACGAAGACGGAGGAGTGAGGAGGTTCACCAGCATACTGGATTTGCCTCCGGATCTATACAAAATCGATGAGATTAGGGCTAAGATGAAGAATGGGGTTGTAGATGAGAGAAGGGAGGGTACTGGTTGCAGTGGATAG

Protein Analysis

89

Amino Acids

9.98

Weight (kDa)

4.86

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 2 - 76 1.7e-12 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018774)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g11472
malus_domestica MD13G1120200.v1.1
rosa_chinensis RchiOBHm_Chr2g0099071
rosa_laevigata RLG00000016843
rosa_samantha Rh2AG125500 Rh2BG128800 Rh2CG129800 Rh2DG130000
rosa_wichuraiana Rw2G009670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 178
AclWI GGATC 1 cut(s) 189
AfaI GTAC 1 cut(s) 250
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 96
AlwI GGATC 1 cut(s) 189
Aor13HI TCCGGA 1 cut(s) 178
AsuHPI GGTGA 1 cut(s) 148
BarI GAAGNNNNNNTAC 2 cut(s) 232, 264
BbsI GAAGAC 1 cut(s) 141
BciT130I CCWGG 1 cut(s) 98
Bme1390I CCNGG 1 cut(s) 98
BmgBI CACGTC 1 cut(s) 70
BmrFI CCNGG 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 141
Bsa29I ATCGAT 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 96
BsaWI WCCGGW 1 cut(s) 178
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bse1I ACTGG 2 cut(s) 171, 256
BseAI TCCGGA 1 cut(s) 178
BseBI CCWGG 1 cut(s) 98
BseCI ATCGAT 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 1 cut(s) 10
BseNI ACTGG 2 cut(s) 171, 256
BseRI GAGGAG 2 cut(s) 156, 163
BshVI ATCGAT 1 cut(s) 195
BsiSI CCGG 1 cut(s) 179
Bsp13I TCCGGA 1 cut(s) 178
Bsp143I GATC 1 cut(s) 181
BspDI ATCGAT 1 cut(s) 195
BspEI TCCGGA 1 cut(s) 178
BspPI GGATC 1 cut(s) 189
BsrI ACTGG 2 cut(s) 171, 256
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 98
BstDEI CTNAG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 96
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 166
BstYI RGATCY 1 cut(s) 181
Bsu15I ATCGAT 1 cut(s) 195
BsuTUI ATCGAT 1 cut(s) 195
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 1 cut(s) 265
ClaI ATCGAT 1 cut(s) 195
Csp6I GTAC 1 cut(s) 249
CviAII CATG 1 cut(s) 46
CviJI RGCY 2 cut(s) 26, 209
CviKI_1 RGCY 2 cut(s) 26, 209
CviQI GTAC 1 cut(s) 249
DdeI CTNAG 1 cut(s) 210
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 96
FaeI CATG 1 cut(s) 49
FaiI YATR 3 cut(s) 47, 164, 187
FatI CATG 1 cut(s) 45
FokI GGATG 1 cut(s) 17
HapII CCGG 1 cut(s) 179
Hin1II CATG 1 cut(s) 49
HinfI GANTC 1 cut(s) 122
HpaII CCGG 1 cut(s) 179
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 156
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 1 cut(s) 179
Hpy8I GTNNAC 1 cut(s) 156
HpyAV CCTTC 3 cut(s) 4, 112, 234
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 1 cut(s) 258
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 1 cut(s) 49
Kpn2I TCCGGA 1 cut(s) 178
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 1 cut(s) 85
LpnPI CCDG 6 cut(s) 83, 110, 152, 172, 192, 237
MaeII ACGT 1 cut(s) 69
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 3 cut(s) 85, 146, 229
MflI RGATCY 1 cut(s) 181
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 9 cut(s) 16, 101, 106, 121, 134, 141, 144, 186, 238
MroI TCCGGA 1 cut(s) 178
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 1 cut(s) 98
MvaI CCWGG 1 cut(s) 98
NdeII GATC 1 cut(s) 181
NlaIII CATG 1 cut(s) 49
NspI RCATGY 1 cut(s) 49
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PsuI RGATCY 1 cut(s) 181
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
Sau3AI GATC 1 cut(s) 181
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 1 cut(s) 98
SetI ASST 2 cut(s) 72, 155
StyD4I CCNGG 1 cut(s) 96
TaiI ACGT 1 cut(s) 72
TaqI TCGA 1 cut(s) 195
TscAI CASTG 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 230
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 1 cut(s) 265
XceI RCATGY 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.