RchiOBHm_Chr2g0099071

Rho (Ras homology) subfamily of Ras-like small GTPases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0018774
Physical Location & Seq
Reverse (-)
11354450 .. 11355361
912 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47380

Sequence Viewer

Length: 705 bp
ATGAGGGAAAATAGTTTGCTAAAAGTGGGCCTATTGGACCATCAGAGCTCCCACAGCCCTGGCCAAACTATAGCGTCAGAAAAAATCTCATTTCTAGAAACTTCTTTCACGAGCTCATTTCTCACTCTCACTGGCTCTGGTCACACTCACTCTCCAAGCTCCAACCTTTATTTTCAACAGAGAGAGAGCTTCAGACCTCCTCCAATGGCGTCCTCTGCAACCCTAATCAACCGATTCCTCCTCACCCGAACCTCATCAAACCCCATCATCCCCTCCTCGATTCGCTCGCTCTCCTCCACACTCTACTGGATCGTGAAGCCAGTGCCGCAGCGCGCGCGTGACGACGCCCCCGACGACCTGCACCGCTCCGGTCTGCTCATCCAAGACAAGGACGACGCGCTCTACCTCCAGAAGTCGATGCCGGGCCTCGGAAAAGATGACGTCGACGTCGAGATTAGTCCCAAGCGCAACACTCTCATCATCACAGGCGAGCCCGGAGGCTACAAGGCGCTTCTTCACCTTCCGCCGTCGTGCCTGGCGGCGGAGGCCGTGTCCAAAATGGAGAATGGTCTGCTGAGGGTGCTTGTGCCCAAGACTAAGCCCAATGGAGCAGGCCCAACTCAGGAGGCCCAATGGGGTCCACCTGGGCACTACAAACCTGGTGACGCGATCCTCATGGTCGGGTACCTCTGCCAAGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632 Ras

Protein Analysis

234

Amino Acids

25.52

Weight (kDa)

7.06

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018774)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g11472
malus_domestica MD13G1120200.v1.1
rosa_chinensis RchiOBHm_Chr2g0099071
rosa_laevigata RLG00000016843
rosa_samantha Rh2AG125500 Rh2BG128800 Rh2CG129800 Rh2DG130000
rosa_wichuraiana Rw2G009670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 446
AatII GACGTC 2 cut(s) 444, 450
Acc36I ACCTGC 1 cut(s) 366
Acc65I GGTACC 1 cut(s) 684
AccB1I GGYRCC 1 cut(s) 684
AccBSI CCGCTC 1 cut(s) 366
AccI GTMKAC 1 cut(s) 444
AccII CGCG 5 cut(s) 333, 335, 337, 398, 668
AciI CCGC 5 cut(s) 326, 364, 524, 539, 542
AclWI GGATC 2 cut(s) 317, 664
AcoI YGGCCR 1 cut(s) 61
AcuI CTGAAG 1 cut(s) 175
AcyI GRCGYC 4 cut(s) 209, 345, 441, 447
AfaI GTAC 1 cut(s) 686
AfiI CCNNNNNNNGG 4 cut(s) 388, 428, 541, 622
AgsI TTSAA 1 cut(s) 176
AjnI CCWGG 4 cut(s) 58, 534, 643, 658
AluBI AGCT 4 cut(s) 48, 114, 159, 189
AluI AGCT 4 cut(s) 48, 114, 159, 189
Alw21I GWGCWC 2 cut(s) 50, 116
AlwI GGATC 2 cut(s) 317, 664
AoxI GGCC 6 cut(s) 28, 61, 424, 546, 613, 627
ApeKI GCWGC 1 cut(s) 328
Asp718I GGTACC 1 cut(s) 684
AspLEI GCGC 6 cut(s) 333, 335, 337, 400, 468, 511
AspS9I GGNCC 6 cut(s) 28, 37, 424, 614, 628, 638
AsuC2I CCSGG 2 cut(s) 423, 495
AsuHPI GGTGA 3 cut(s) 235, 509, 674
AvaII GGWCC 2 cut(s) 37, 638
BaeGI GKGCMC 2 cut(s) 591, 651
BalI TGGCCA 1 cut(s) 63
BanI GGYRCC 1 cut(s) 684
BanII GRGCYC 3 cut(s) 50, 116, 495
BauI CACGAG 1 cut(s) 109
Bbv12I GWGCWC 2 cut(s) 50, 116
BbvCI CCTCAGC 1 cut(s) 575
BbvI GCAGC 1 cut(s) 340
BccI CCATC 2 cut(s) 48, 272
BceAI ACGGC 2 cut(s) 511, 533
BciT130I CCWGG 4 cut(s) 60, 536, 645, 660
BcnI CCSGG 2 cut(s) 423, 495
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 69
BfoI RGCGCY 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 366
BisI GCNGC 3 cut(s) 326, 329, 540
BlsI GCNGC 3 cut(s) 327, 330, 541
Bme1390I CCNGG 6 cut(s) 60, 423, 495, 536, 645, 660
Bme18I GGWCC 2 cut(s) 37, 638
BmgT120I GGNCC 6 cut(s) 28, 37, 424, 614, 628, 638
BmiI GGNNCC 2 cut(s) 639, 686
BmrFI CCNGG 6 cut(s) 60, 423, 495, 536, 645, 660
BmsI GCATC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 392
Bpu10I CCTNAGC 1 cut(s) 575
BpuMI CCSGG 2 cut(s) 423, 495
BsaHI GRCGYC 4 cut(s) 209, 345, 441, 447
BsaJI CCNNGG 3 cut(s) 58, 427, 644
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 536, 566
Bsc4I CCNNNNNNNGG 4 cut(s) 388, 428, 541, 622
Bse1I ACTGG 3 cut(s) 136, 311, 320
BseBI CCWGG 4 cut(s) 60, 536, 645, 660
BseDI CCNNGG 3 cut(s) 58, 427, 644
BseGI GGATG 2 cut(s) 267, 378
BseLI CCNNNNNNNGG 4 cut(s) 388, 428, 541, 622
BseMII CTCAG 2 cut(s) 566, 635
BseNI ACTGG 3 cut(s) 136, 311, 320
BsePI GCGCGC 2 cut(s) 331, 333
BseRI GAGGAG 4 cut(s) 189, 230, 265, 283
BseSI GKGCMC 2 cut(s) 591, 651
BseXI GCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 344
Bsh1236I CGCG 5 cut(s) 333, 335, 337, 398, 668
BshFI GGCC 6 cut(s) 30, 63, 426, 548, 615, 629
BshNI GGYRCC 1 cut(s) 684
BsiHKAI GWGCWC 2 cut(s) 50, 116
BsiSI CCGG 3 cut(s) 369, 422, 495
BslFI GGGAC 1 cut(s) 444
BslI CCNNNNNNNGG 4 cut(s) 388, 428, 541, 622
BsmFI GGGAC 1 cut(s) 444
BsnI GGCC 6 cut(s) 30, 63, 426, 548, 615, 629
Bsp1286I GDGCHC 5 cut(s) 50, 116, 495, 591, 651
Bsp143I GATC 2 cut(s) 309, 669
BspACI CCGC 5 cut(s) 326, 364, 524, 539, 542
BspANI GGCC 6 cut(s) 30, 63, 426, 548, 615, 629
BspCNI CTCAG 2 cut(s) 567, 634
BspFNI CGCG 5 cut(s) 333, 335, 337, 398, 668
BspLI GGNNCC 2 cut(s) 639, 686
BspMI ACCTGC 1 cut(s) 366
BspPI GGATC 2 cut(s) 317, 664
BspT107I GGYRCC 1 cut(s) 684
BsrBI CCGCTC 1 cut(s) 366
BsrI ACTGG 3 cut(s) 136, 311, 320
BssECI CCNNGG 3 cut(s) 58, 427, 644
BssHII GCGCGC 2 cut(s) 331, 333
BssMI GATC 2 cut(s) 309, 669
BssNI GRCGYC 4 cut(s) 209, 345, 441, 447
BssSI CACGAG 1 cut(s) 109
Bst2BI CACGAG 1 cut(s) 109
Bst2UI CCWGG 4 cut(s) 60, 536, 645, 660
BstACI GRCGYC 4 cut(s) 209, 345, 441, 447
BstC8I GCNNGC 5 cut(s) 287, 333, 335, 491, 613
BstDEI CTNAG 3 cut(s) 575, 597, 621
BstF5I GGATG 2 cut(s) 267, 378
BstFNI CGCG 5 cut(s) 333, 335, 337, 398, 668
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 6 cut(s) 333, 335, 337, 400, 468, 511
BstKTI GATC 2 cut(s) 312, 672
BstMBI GATC 2 cut(s) 309, 669
BstMWI GCNNNNNNNGC 6 cut(s) 54, 215, 325, 334, 545, 580
BstNI CCWGG 4 cut(s) 60, 536, 645, 660
BstSCI CCNGG 6 cut(s) 58, 421, 493, 534, 643, 658
BstSFI CTRYAG 1 cut(s) 69
BstSLI GKGCMC 2 cut(s) 591, 651
BstUI CGCG 5 cut(s) 333, 335, 337, 398, 668
BstV1I GCAGC 1 cut(s) 340
BstXI CCANNNNNNTGG 1 cut(s) 59
BsuRI GGCC 6 cut(s) 30, 63, 426, 548, 615, 629
BtsCI GGATG 2 cut(s) 267, 378
BtsIMutI CAGTG 2 cut(s) 129, 327
BveI ACCTGC 1 cut(s) 366
Cac8I GCNNGC 5 cut(s) 287, 333, 335, 491, 613
CfoI GCGC 6 cut(s) 333, 335, 337, 400, 468, 511
Cfr13I GGNCC 6 cut(s) 28, 37, 424, 614, 628, 638
CseI GACGC 5 cut(s) 63, 198, 353, 404, 674
CsiI ACCWGGT 1 cut(s) 658
Csp6I GTAC 1 cut(s) 685
CviAII CATG 1 cut(s) 676
CviQI GTAC 1 cut(s) 685
DdeI CTNAG 3 cut(s) 575, 597, 621
DpnI GATC 2 cut(s) 311, 671
DpnII GATC 2 cut(s) 309, 669
DrdI GACNNNNNNGTC 1 cut(s) 446
DseDI GACNNNNNNGTC 1 cut(s) 446
EaeI YGGCCR 1 cut(s) 61
EciI GGCGGA 2 cut(s) 513, 557
Ecl136II GAGCTC 2 cut(s) 48, 114
Eco24I GRGCYC 3 cut(s) 50, 116, 495
Eco47I GGWCC 2 cut(s) 37, 638
Eco53kI GAGCTC 2 cut(s) 48, 114
Eco57I CTGAAG 1 cut(s) 175
EcoICRI GAGCTC 2 cut(s) 48, 114
EcoRII CCWGG 4 cut(s) 58, 534, 643, 658
EcoT38I GRGCYC 3 cut(s) 50, 116, 495
FaeI CATG 1 cut(s) 679
FaiI YATR 2 cut(s) 71, 677
FaqI GGGAC 1 cut(s) 444
FatI CATG 1 cut(s) 675
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 3 cut(s) 326, 329, 540
FokI GGATG 2 cut(s) 254, 365
FriOI GRGCYC 3 cut(s) 50, 116, 495
Fsp4HI GCNGC 3 cut(s) 326, 329, 540
FspBI CTAG 1 cut(s) 95
GlaI GCGC 6 cut(s) 332, 334, 336, 399, 467, 510
GluI GCNGC 3 cut(s) 326, 329, 540
GsuI CTGGAG 1 cut(s) 392
HaeII RGCGCY 1 cut(s) 512
HaeIII GGCC 6 cut(s) 30, 63, 426, 548, 615, 629
HapII CCGG 3 cut(s) 369, 422, 495
HgaI GACGC 5 cut(s) 63, 198, 353, 404, 674
HhaI GCGC 6 cut(s) 333, 335, 337, 400, 468, 511
Hin1I GRCGYC 4 cut(s) 209, 345, 441, 447
Hin1II CATG 1 cut(s) 679
Hin6I GCGC 6 cut(s) 331, 333, 335, 398, 466, 509
HinP1I GCGC 6 cut(s) 331, 333, 335, 398, 466, 509
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HinfI GANTC 2 cut(s) 234, 280
HpaII CCGG 3 cut(s) 369, 422, 495
HphI GGTGA 3 cut(s) 235, 509, 674
Hpy166II GTNNAC 2 cut(s) 445, 641
Hpy188I TCNGA 4 cut(s) 45, 79, 194, 431
Hpy188III TCNNGA 6 cut(s) 95, 109, 313, 409, 451, 623
Hpy8I GTNNAC 2 cut(s) 445, 641
Hpy99I CGWCG 7 cut(s) 347, 356, 398, 446, 449, 452, 532
HpyAV CCTTC 1 cut(s) 530
HpyCH4IV ACGT 2 cut(s) 441, 447
HpyCH4V TGCA 2 cut(s) 218, 361
HpyF10VI GCNNNNNNNGC 6 cut(s) 54, 215, 325, 334, 545, 580
HpyF3I CTNAG 3 cut(s) 575, 597, 621
HpySE526I ACGT 2 cut(s) 441, 447
Hsp92I GRCGYC 4 cut(s) 209, 345, 441, 447
Hsp92II CATG 1 cut(s) 679
HspAI GCGC 6 cut(s) 331, 333, 335, 398, 466, 509
KpnI GGTACC 1 cut(s) 688
Kzo9I GATC 2 cut(s) 309, 669
LmnI GCTCC 4 cut(s) 53, 164, 371, 608
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 1 cut(s) 408
MabI ACCWGGT 1 cut(s) 658
MaeI CTAG 1 cut(s) 95
MaeII ACGT 2 cut(s) 441, 447
MaeIII GTNAC 3 cut(s) 140, 338, 662
MalI GATC 2 cut(s) 311, 671
MauBI CGCGCGCG 1 cut(s) 333
MbiI CCGCTC 1 cut(s) 366
MboI GATC 2 cut(s) 309, 669
MboII GAAGA 1 cut(s) 506
MhlI GDGCHC 5 cut(s) 50, 116, 495, 591, 651
MlsI TGGCCA 1 cut(s) 63
MluNI TGGCCA 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 186
Mox20I TGGCCA 1 cut(s) 63
MscI TGGCCA 1 cut(s) 63
Msp20I TGGCCA 1 cut(s) 63
MspI CCGG 3 cut(s) 369, 422, 495
MspR9I CCNGG 6 cut(s) 60, 423, 495, 536, 645, 660
MvaI CCWGG 4 cut(s) 60, 536, 645, 660
MvnI CGCG 5 cut(s) 333, 335, 337, 398, 668
MwoI GCNNNNNNNGC 6 cut(s) 54, 215, 325, 334, 545, 580
NciI CCSGG 2 cut(s) 423, 495
NdeII GATC 2 cut(s) 309, 669
NlaIII CATG 1 cut(s) 679
NlaIV GGNNCC 2 cut(s) 639, 686
NmuCI GTSAC 3 cut(s) 140, 338, 662
PauI GCGCGC 2 cut(s) 331, 333
PcsI WCGNNNNNNNCGW 3 cut(s) 348, 351, 447
PfeI GAWTC 2 cut(s) 234, 280
PkrI GCNGC 3 cut(s) 327, 330, 541
Psp124BI GAGCTC 2 cut(s) 50, 116
Psp6I CCWGG 4 cut(s) 58, 534, 643, 658
PspGI CCWGG 4 cut(s) 58, 534, 643, 658
PspN4I GGNNCC 2 cut(s) 639, 686
PspPI GGNCC 6 cut(s) 28, 37, 424, 614, 628, 638
PteI GCGCGC 2 cut(s) 331, 333
RsaI GTAC 1 cut(s) 686
RsaNI GTAC 1 cut(s) 685
SacI GAGCTC 2 cut(s) 50, 116
SalI GTCGAC 1 cut(s) 443
SatI GCNGC 3 cut(s) 326, 329, 540
Sau3AI GATC 2 cut(s) 309, 669
Sau96I GGNCC 6 cut(s) 28, 37, 424, 614, 628, 638
ScrFI CCNGG 6 cut(s) 60, 423, 495, 536, 645, 660
SduI GDGCHC 5 cut(s) 50, 116, 495, 591, 651
SexAI ACCWGGT 1 cut(s) 658
SfaNI GCATC 1 cut(s) 408
SfcI CTRYAG 1 cut(s) 69
SgrDI CGTCGACG 1 cut(s) 443
SinI GGWCC 2 cut(s) 37, 638
SsiI CCGC 5 cut(s) 326, 364, 524, 539, 542
SspMI CTAG 1 cut(s) 95
SstI GAGCTC 2 cut(s) 50, 116
StyD4I CCNGG 6 cut(s) 58, 421, 493, 534, 643, 658
TaiI ACGT 2 cut(s) 444, 450
TaqI TCGA 4 cut(s) 278, 416, 444, 450
TauI GCSGC 2 cut(s) 328, 542
TfiI GAWTC 2 cut(s) 234, 280
TscAI CASTG 2 cut(s) 136, 327
TseFI GTSAC 3 cut(s) 140, 338, 662
TseI GCWGC 1 cut(s) 328
Tsp45I GTSAC 3 cut(s) 140, 338, 662
TspRI CASTG 2 cut(s) 136, 327
VpaK11BI GGWCC 2 cut(s) 37, 638
XbaI TCTAGA 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 444
XspI CTAG 1 cut(s) 95
ZraI GACGTC 2 cut(s) 442, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.