RLG00000016843

Rho (Ras homology) subfamily of Ras-like small GTPases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018774
Physical Location & Seq
Reverse (-)
10585343 .. 10585852
510 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016843

Sequence Viewer

Length: 510 bp
ATGGCGTCCTCTGCAACCCTAATCAACCGATTCCTCCTCACGCGAACCTCATCAAACCCCATCCCCTCCTCGATTCGCTCGCTCTCCTCCACACTCTACTGGATCGTGAAGCCAGTGCCGCAGCGCGCGCGTCACGACGCCCCAGACGACCTGCACCGCTCCGGTCTGCTCATCCAAGACGAGGACGACGCGCTCTACCTCCAGAAGTCGATGCCGGGGCTCGGAAAAGATGACGTCGACGTCGAGATTAGTCCCAAGCGCAACACTCTCATCATCACAGGAGAGCCCGGAGGCTACAAGGCGCTTCTTCACCTTCCGCCGTCGTGCCAGGCGGAGGAGGCCGTGTCCAAAATGGAGAATGGTCTGCTGAGGGTGATTGTGCCCAAGACTAAGCCCAATGGAGCAGGAGGAGGAGGAGGCCCAACTGAAGAGGCCCAATGGGGTCCACCTGGGCACTACAAGCCTGGTGACGCTATCCTCATGGTCGGGTACCTCCGCCAAGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632 Ras

Protein Analysis

170

Amino Acids

18.26

Weight (kDa)

6.08

Isoelectric Point (pI)

56.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018774)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g11472
malus_domestica MD13G1120200.v1.1
rosa_chinensis RchiOBHm_Chr2g0099071
rosa_laevigata RLG00000016843
rosa_samantha Rh2AG125500 Rh2BG128800 Rh2CG129800 Rh2DG130000
rosa_wichuraiana Rw2G009670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AatII GACGTC 2 cut(s) 237, 243
Acc36I ACCTGC 1 cut(s) 159
Acc65I GGTACC 1 cut(s) 489
AccB1I GGYRCC 1 cut(s) 489
AccBSI CCGCTC 1 cut(s) 159
AccI GTMKAC 1 cut(s) 237
AccII CGCG 5 cut(s) 43, 126, 128, 130, 191
AciI CCGC 5 cut(s) 119, 157, 317, 332, 496
AclWI GGATC 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 447
AcyI GRCGYC 4 cut(s) 5, 138, 234, 240
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 3 cut(s) 181, 221, 334
AjnI CCWGG 3 cut(s) 327, 448, 463
AlwI GGATC 1 cut(s) 110
AoxI GGCC 3 cut(s) 339, 418, 432
ApeKI GCWGC 1 cut(s) 121
Asp718I GGTACC 1 cut(s) 489
AspLEI GCGC 6 cut(s) 126, 128, 130, 193, 261, 304
AspS9I GGNCC 3 cut(s) 419, 433, 443
AsuC2I CCSGG 2 cut(s) 216, 288
AsuHPI GGTGA 3 cut(s) 302, 385, 479
AvaII GGWCC 1 cut(s) 443
BaeGI GKGCMC 2 cut(s) 384, 456
BanI GGYRCC 1 cut(s) 489
BanII GRGCYC 2 cut(s) 222, 288
BbvCI CCTCAGC 1 cut(s) 368
BbvI GCAGC 1 cut(s) 133
BccI CCATC 1 cut(s) 68
BceAI ACGGC 2 cut(s) 304, 326
BciT130I CCWGG 3 cut(s) 329, 450, 465
BcnI CCSGG 2 cut(s) 216, 288
BfoI RGCGCY 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 159
BisI GCNGC 2 cut(s) 119, 122
BlsI GCNGC 2 cut(s) 120, 123
Bme1390I CCNGG 5 cut(s) 216, 288, 329, 450, 465
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 3 cut(s) 419, 433, 443
BmiI GGNNCC 2 cut(s) 444, 491
BmrFI CCNGG 5 cut(s) 216, 288, 329, 450, 465
BmsI GCATC 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 185
Bpu10I CCTNAGC 1 cut(s) 368
BpuMI CCSGG 2 cut(s) 216, 288
BsaHI GRCGYC 4 cut(s) 5, 138, 234, 240
BsaJI CCNNGG 2 cut(s) 215, 449
BsaWI WCCGGW 1 cut(s) 161
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 221, 334
Bse1I ACTGG 2 cut(s) 104, 113
BseBI CCWGG 3 cut(s) 329, 450, 465
BseDI CCNNGG 2 cut(s) 215, 449
BseGI GGATG 2 cut(s) 60, 171
BseLI CCNNNNNNNGG 3 cut(s) 181, 221, 334
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 2 cut(s) 104, 113
BsePI GCGCGC 2 cut(s) 124, 126
BseRI GAGGAG 7 cut(s) 26, 58, 76, 350, 423, 426, 429
BseSI GKGCMC 2 cut(s) 384, 456
BseXI GCAGC 1 cut(s) 133
BsgI GTGCAG 1 cut(s) 137
Bsh1236I CGCG 5 cut(s) 43, 126, 128, 130, 191
BshFI GGCC 3 cut(s) 341, 420, 434
BshNI GGYRCC 1 cut(s) 489
BsiSI CCGG 3 cut(s) 162, 215, 288
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 3 cut(s) 181, 221, 334
BsmFI GGGAC 1 cut(s) 237
BsnI GGCC 3 cut(s) 341, 420, 434
Bsp1286I GDGCHC 4 cut(s) 222, 288, 384, 456
Bsp143I GATC 1 cut(s) 102
BspACI CCGC 5 cut(s) 119, 157, 317, 332, 496
BspANI GGCC 3 cut(s) 341, 420, 434
BspCNI CTCAG 1 cut(s) 360
BspFNI CGCG 5 cut(s) 43, 126, 128, 130, 191
BspLI GGNNCC 2 cut(s) 444, 491
BspMI ACCTGC 1 cut(s) 159
BspPI GGATC 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 489
BsrBI CCGCTC 1 cut(s) 159
BsrI ACTGG 2 cut(s) 104, 113
BssECI CCNNGG 2 cut(s) 215, 449
BssHII GCGCGC 2 cut(s) 124, 126
BssMI GATC 1 cut(s) 102
BssNI GRCGYC 4 cut(s) 5, 138, 234, 240
Bst2UI CCWGG 3 cut(s) 329, 450, 465
Bst6I CTCTTC 1 cut(s) 423
BstACI GRCGYC 4 cut(s) 5, 138, 234, 240
BstC8I GCNNGC 3 cut(s) 80, 126, 128
BstDEI CTNAG 2 cut(s) 368, 390
BstF5I GGATG 2 cut(s) 60, 171
BstFNI CGCG 5 cut(s) 43, 126, 128, 130, 191
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 6 cut(s) 126, 128, 130, 193, 261, 304
BstKTI GATC 1 cut(s) 105
BstMBI GATC 1 cut(s) 102
BstMWI GCNNNNNNNGC 5 cut(s) 11, 118, 127, 338, 460
BstNI CCWGG 3 cut(s) 329, 450, 465
BstSCI CCNGG 5 cut(s) 214, 286, 327, 448, 463
BstSLI GKGCMC 2 cut(s) 384, 456
BstUI CGCG 5 cut(s) 43, 126, 128, 130, 191
BstV1I GCAGC 1 cut(s) 133
BsuRI GGCC 3 cut(s) 341, 420, 434
BtsCI GGATG 2 cut(s) 60, 171
BtsIMutI CAGTG 1 cut(s) 120
BveI ACCTGC 1 cut(s) 159
Cac8I GCNNGC 3 cut(s) 80, 126, 128
CfoI GCGC 6 cut(s) 126, 128, 130, 193, 261, 304
Cfr13I GGNCC 3 cut(s) 419, 433, 443
CseI GACGC 4 cut(s) 119, 146, 197, 479
Csp6I GTAC 1 cut(s) 490
CviAII CATG 1 cut(s) 481
CviJI RGCY 9 cut(s) 112, 220, 286, 294, 341, 394, 420, 434, 463
CviKI_1 RGCY 9 cut(s) 112, 220, 286, 294, 341, 394, 420, 434, 463
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 2 cut(s) 368, 390
DpnI GATC 1 cut(s) 104
DpnII GATC 1 cut(s) 102
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
EciI GGCGGA 3 cut(s) 306, 347, 485
Eco24I GRGCYC 2 cut(s) 222, 288
Eco47I GGWCC 1 cut(s) 443
Eco57I CTGAAG 1 cut(s) 447
EcoRII CCWGG 3 cut(s) 327, 448, 463
EcoT38I GRGCYC 2 cut(s) 222, 288
FaeI CATG 1 cut(s) 484
FaiI YATR 1 cut(s) 482
FaqI GGGAC 1 cut(s) 237
FatI CATG 1 cut(s) 480
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 2 cut(s) 119, 122
FokI GGATG 2 cut(s) 47, 158
FriOI GRGCYC 2 cut(s) 222, 288
Fsp4HI GCNGC 2 cut(s) 119, 122
GlaI GCGC 6 cut(s) 125, 127, 129, 192, 260, 303
GluI GCNGC 2 cut(s) 119, 122
GsuI CTGGAG 1 cut(s) 185
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 3 cut(s) 341, 420, 434
HapII CCGG 3 cut(s) 162, 215, 288
HgaI GACGC 4 cut(s) 119, 146, 197, 479
HhaI GCGC 6 cut(s) 126, 128, 130, 193, 261, 304
Hin1I GRCGYC 4 cut(s) 5, 138, 234, 240
Hin1II CATG 1 cut(s) 484
Hin6I GCGC 6 cut(s) 124, 126, 128, 191, 259, 302
HinP1I GCGC 6 cut(s) 124, 126, 128, 191, 259, 302
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 2 cut(s) 30, 73
HpaII CCGG 3 cut(s) 162, 215, 288
HphI GGTGA 3 cut(s) 302, 385, 479
Hpy166II GTNNAC 2 cut(s) 238, 446
Hpy188I TCNGA 1 cut(s) 224
Hpy188III TCNNGA 4 cut(s) 106, 134, 202, 244
Hpy8I GTNNAC 2 cut(s) 238, 446
Hpy99I CGWCG 6 cut(s) 140, 191, 239, 242, 245, 325
HpyAV CCTTC 1 cut(s) 323
HpyCH4IV ACGT 2 cut(s) 234, 240
HpyCH4V TGCA 2 cut(s) 14, 154
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 118, 127, 338, 460
HpyF3I CTNAG 2 cut(s) 368, 390
HpySE526I ACGT 2 cut(s) 234, 240
Hsp92I GRCGYC 4 cut(s) 5, 138, 234, 240
Hsp92II CATG 1 cut(s) 484
HspAI GCGC 6 cut(s) 124, 126, 128, 191, 259, 302
KpnI GGTACC 1 cut(s) 493
Kzo9I GATC 1 cut(s) 102
LmnI GCTCC 2 cut(s) 164, 401
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 1 cut(s) 201
MaeII ACGT 2 cut(s) 234, 240
MaeIII GTNAC 2 cut(s) 131, 467
MalI GATC 1 cut(s) 104
MauBI CGCGCGCG 1 cut(s) 126
MbiI CCGCTC 1 cut(s) 159
MboI GATC 1 cut(s) 102
MboII GAAGA 2 cut(s) 299, 440
MhlI GDGCHC 4 cut(s) 222, 288, 384, 456
MspI CCGG 3 cut(s) 162, 215, 288
MspR9I CCNGG 5 cut(s) 216, 288, 329, 450, 465
MvaI CCWGG 3 cut(s) 329, 450, 465
MvnI CGCG 5 cut(s) 43, 126, 128, 130, 191
MwoI GCNNNNNNNGC 5 cut(s) 11, 118, 127, 338, 460
NciI CCSGG 2 cut(s) 216, 288
NdeII GATC 1 cut(s) 102
NlaIII CATG 1 cut(s) 484
NlaIV GGNNCC 2 cut(s) 444, 491
NmuCI GTSAC 2 cut(s) 131, 467
PauI GCGCGC 2 cut(s) 124, 126
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 240
PfeI GAWTC 2 cut(s) 30, 73
PkrI GCNGC 2 cut(s) 120, 123
Psp6I CCWGG 3 cut(s) 327, 448, 463
PspGI CCWGG 3 cut(s) 327, 448, 463
PspN4I GGNNCC 2 cut(s) 444, 491
PspPI GGNCC 3 cut(s) 419, 433, 443
PteI GCGCGC 2 cut(s) 124, 126
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
SalI GTCGAC 1 cut(s) 236
SatI GCNGC 2 cut(s) 119, 122
Sau3AI GATC 1 cut(s) 102
Sau96I GGNCC 3 cut(s) 419, 433, 443
ScrFI CCNGG 5 cut(s) 216, 288, 329, 450, 465
SduI GDGCHC 4 cut(s) 222, 288, 384, 456
SetI ASST 8 cut(s) 50, 153, 201, 237, 243, 315, 451, 495
SfaNI GCATC 1 cut(s) 201
SgrDI CGTCGACG 1 cut(s) 236
SinI GGWCC 1 cut(s) 443
SsiI CCGC 5 cut(s) 119, 157, 317, 332, 496
StyD4I CCNGG 5 cut(s) 214, 286, 327, 448, 463
TaiI ACGT 2 cut(s) 237, 243
TaqI TCGA 4 cut(s) 71, 209, 237, 243
TauI GCSGC 1 cut(s) 121
TfiI GAWTC 2 cut(s) 30, 73
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 2 cut(s) 131, 467
TseI GCWGC 1 cut(s) 121
Tsp45I GTSAC 2 cut(s) 131, 467
TspRI CASTG 1 cut(s) 120
VpaK11BI GGWCC 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 237
ZraI GACGTC 2 cut(s) 235, 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.