MD13G1129600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015310
Physical Location & Seq
Forward (+)
9795900 .. 9802954
7055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1129600.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGTGCAGAGATTTTTCTCGGTTGGGCAAATGGACCTCCAACAAAACCATCACTCCCACCCTATTCTGCTTCTCTCCGGCCCTCCTTCCTGCGGAAAGACCTCCTTATTATTCCAGTTTGCATTCAACTCCGCAATGGAGGCCGCCCGCGATTGCAAAAATGGTCAAGTGGTCTTCATCTGCAACCGACGCAGATTAGAGGCCAAACCCCCTTATCTTTCCCAGGGCGTTGATTCATCCTCTGAAGCATTTCAACGAATACAAATGAAGTATGTGGACGATGAGGAAGGAATTAAGAAGTATTTTGCTGCATTTCATCTGCACGATACATTTCCGGTCGCAGTTGTCATCGATGATTTTGGAGCTTTCTTTGATGAAAGGAGCTGCCAAGAAAGATATGGAAACCCACGAGGAAGAGACTTGGCAATGGTTCGGACACTGGCTTTGTGTCACAATGCCATGATCCATGCCAATGAAACAAGGCCTTGCAAGCTTGTGTTATCAGATACACACCGCGGAGACTCCCCGCGGTTGCTCTTCATCTACAAAAGATGGGTTTCGACCATTTTTACAATTCAAGGCGATGGCTCAGGATCGTTCATTGTAAAAGACTATAGCAATTCAGGGGGAAGGACTGCTAAGTACTCGTTAGCTCTCCAGAATCTTTTGCTAGTGGAGATCACTGAAGCAAGTGAATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

234

Amino Acids

26.27

Weight (kDa)

7.67

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23255 AT3G23255
fragaria_vesca FvH4_4g22791 FvH4_4g22791
malus_domestica MD13G1129600.v1.1 MD16G1129800.v1.1
prunus_persica Prupe.1G216800_v2.0.a1
pyrus_communis pycom16g11000
rosa_chinensis RchiOBHm_Chr4g0429021
rosa_laevigata RLG00000007103
rosa_roxburghii Rroxscaffold_5G00370670
rosa_rugosa Rorug04G0232100
rosa_samantha Rh4BG294300 Rh4CG309800 Rh4DG291200
rosa_wichuraiana Rw4G024970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 150, 516, 529
AciI CCGC 8 cut(s) 93, 132, 144, 148, 514, 516, 527, 529
AclWI GGATC 2 cut(s) 457, 601
AcuI CTGAAG 1 cut(s) 264
AfaI GTAC 1 cut(s) 644
AfiI CCNNNNNNNGG 1 cut(s) 92
AgsI TTSAA 3 cut(s) 127, 254, 578
AjnI CCWGG 1 cut(s) 222
AluBI AGCT 4 cut(s) 365, 384, 493, 653
AluI AGCT 4 cut(s) 365, 384, 493, 653
Alw26I GTCTC 2 cut(s) 411, 513
AlwI GGATC 2 cut(s) 457, 601
AoxI GGCC 4 cut(s) 79, 141, 201, 482
ApeKI GCWGC 2 cut(s) 308, 384
ArsI GACNNNNNNTTYG 2 cut(s) 427, 459
Asp700I GAANNNNTTC 1 cut(s) 249
AspS9I GGNCC 2 cut(s) 34, 80
AvaII GGWCC 1 cut(s) 34
BauI CACGAG 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 295, 371
BccI CCATC 3 cut(s) 57, 546, 578
BcgI CGANNNNNNTGC 1 cut(s) 678
BciT130I CCWGG 1 cut(s) 224
BcoDI GTCTC 2 cut(s) 411, 513
BfaI CTAG 1 cut(s) 671
BfmI CTRYAG 1 cut(s) 613
BisI GCNGC 3 cut(s) 144, 309, 385
BlsI GCNGC 3 cut(s) 145, 310, 386
BmcAI AGTACT 1 cut(s) 644
Bme1390I CCNGG 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 34
BmgT120I GGNCC 2 cut(s) 34, 80
BmrFI CCNGG 1 cut(s) 224
BpiI GAAGAC 1 cut(s) 166
BpmI CTGGAG 1 cut(s) 641
Bpu10I CCTNAGC 1 cut(s) 589
Bsa29I ATCGAT 1 cut(s) 351
BsaJI CCNNGG 4 cut(s) 222, 223, 514, 527
BsaWI WCCGGW 1 cut(s) 334
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 115, 444
Bse3DI GCAATG 2 cut(s) 141, 432
BseBI CCWGG 1 cut(s) 224
BseCI ATCGAT 1 cut(s) 351
BseDI CCNNGG 4 cut(s) 222, 223, 514, 527
BseGI GGATG 1 cut(s) 236
BseLI CCNNNNNNNGG 1 cut(s) 92
BseMI GCAATG 2 cut(s) 141, 432
BseMII CTCAG 1 cut(s) 603
BseNI ACTGG 2 cut(s) 115, 444
BseXI GCAGC 2 cut(s) 295, 371
BsgI GTGCAG 2 cut(s) 26, 305
Bsh1236I CGCG 3 cut(s) 150, 516, 529
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 4 cut(s) 81, 143, 203, 484
BshVI ATCGAT 1 cut(s) 351
BsiEI CGRYCG 1 cut(s) 339
BsiSI CCGG 2 cut(s) 78, 335
BslI CCNNNNNNNGG 1 cut(s) 92
BsmAI GTCTC 2 cut(s) 411, 513
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 4 cut(s) 81, 143, 203, 484
Bsp143I GATC 3 cut(s) 462, 593, 678
BspACI CCGC 8 cut(s) 93, 132, 144, 148, 514, 516, 527, 529
BspANI GGCC 4 cut(s) 81, 143, 203, 484
BspCNI CTCAG 1 cut(s) 602
BspDI ATCGAT 1 cut(s) 351
BspFNI CGCG 3 cut(s) 150, 516, 529
BspPI GGATC 2 cut(s) 457, 601
BspQI GCTCTTC 1 cut(s) 542
BsrDI GCAATG 2 cut(s) 141, 432
BsrI ACTGG 2 cut(s) 115, 444
BssECI CCNNGG 4 cut(s) 222, 223, 514, 527
BssMI GATC 3 cut(s) 462, 593, 678
BssSI CACGAG 1 cut(s) 408
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 224
Bst6I CTCTTC 2 cut(s) 409, 542
BstC8I GCNNGC 2 cut(s) 148, 491
BstDEI CTNAG 2 cut(s) 589, 639
BstDSI CCRYGG 2 cut(s) 514, 527
BstF5I GGATG 1 cut(s) 236
BstFNI CGCG 3 cut(s) 150, 516, 529
BstKTI GATC 3 cut(s) 465, 596, 681
BstMAI GTCTC 2 cut(s) 411, 513
BstMBI GATC 3 cut(s) 462, 593, 678
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 3 cut(s) 140, 189, 490
BstNI CCWGG 1 cut(s) 224
BstSCI CCNGG 1 cut(s) 222
BstSFI CTRYAG 1 cut(s) 613
BstUI CGCG 3 cut(s) 150, 516, 529
BstV1I GCAGC 2 cut(s) 295, 371
BstV2I GAAGAC 1 cut(s) 166
Bsu15I ATCGAT 1 cut(s) 351
BsuRI GGCC 4 cut(s) 81, 143, 203, 484
BsuTUI ATCGAT 1 cut(s) 351
BtgI CCRYGG 2 cut(s) 514, 527
BtgZI GCGATG 1 cut(s) 597
BtsCI GGATG 1 cut(s) 236
BtsIMutI CAGTG 2 cut(s) 437, 681
Cac8I GCNNGC 2 cut(s) 148, 491
Cfr13I GGNCC 2 cut(s) 34, 80
Cfr42I CCGCGG 2 cut(s) 517, 530
ClaI ATCGAT 1 cut(s) 351
CseI GACGC 1 cut(s) 198
Csp6I GTAC 1 cut(s) 643
CviAII CATG 2 cut(s) 460, 467
CviQI GTAC 1 cut(s) 643
DdeI CTNAG 2 cut(s) 589, 639
DpnI GATC 3 cut(s) 464, 595, 680
DpnII GATC 3 cut(s) 462, 593, 678
Eam1104I CTCTTC 2 cut(s) 409, 542
EarI CTCTTC 2 cut(s) 409, 542
Eco147I AGGCCT 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 222
FaeI CATG 2 cut(s) 463, 470
FaiI YATR 5 cut(s) 273, 399, 461, 468, 615
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FatI CATG 2 cut(s) 459, 466
FauI CCCGC 2 cut(s) 155, 534
Fnu4HI GCNGC 3 cut(s) 144, 309, 385
FokI GGATG 1 cut(s) 223
Fsp4HI GCNGC 3 cut(s) 144, 309, 385
FspBI CTAG 1 cut(s) 671
GluI GCNGC 3 cut(s) 144, 309, 385
GsuI CTGGAG 1 cut(s) 641
HaeIII GGCC 4 cut(s) 81, 143, 203, 484
HapII CCGG 2 cut(s) 78, 335
HgaI GACGC 1 cut(s) 198
Hin1II CATG 2 cut(s) 463, 470
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 4 cut(s) 233, 521, 661, 695
HpaII CCGG 2 cut(s) 78, 335
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 3 cut(s) 244, 435, 505
Hpy188III TCNNGA 2 cut(s) 591, 658
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 1 cut(s) 192
HpyAV CCTTC 3 cut(s) 96, 281, 624
HpyCH4V TGCA 7 cut(s) 7, 122, 156, 183, 311, 322, 489
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 189, 490
HpyF3I CTNAG 2 cut(s) 589, 639
Hsp92II CATG 2 cut(s) 463, 470
KspI CCGCGG 2 cut(s) 517, 530
Kzo9I GATC 3 cut(s) 462, 593, 678
LguI GCTCTTC 1 cut(s) 542
LmnI GCTCC 2 cut(s) 362, 381
Lsp1109I GCAGC 2 cut(s) 295, 371
MaeI CTAG 1 cut(s) 671
MaeIII GTNAC 1 cut(s) 449
MalI GATC 3 cut(s) 464, 595, 680
MboI GATC 3 cut(s) 462, 593, 678
MboII GAAGA 3 cut(s) 166, 426, 529
MluCI AATT 3 cut(s) 291, 573, 619
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 8 cut(s) 47, 93, 112, 133, 193, 250, 277, 404
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 471
MspA1I CMGCKG 2 cut(s) 516, 529
MspI CCGG 2 cut(s) 78, 335
MspR9I CCNGG 1 cut(s) 224
Mva1269I GAATGC 1 cut(s) 122
MvaI CCWGG 1 cut(s) 224
MvnI CGCG 3 cut(s) 150, 516, 529
MwoI GCNNNNNNNGC 3 cut(s) 140, 189, 490
NdeII GATC 3 cut(s) 462, 593, 678
NlaIII CATG 2 cut(s) 463, 470
NmuCI GTSAC 1 cut(s) 449
PasI CCCWGGG 1 cut(s) 223
PceI AGGCCT 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 542
PctI GAATGC 1 cut(s) 122
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 3 cut(s) 233, 661, 695
PkrI GCNGC 3 cut(s) 145, 310, 386
PleI GAGTC 1 cut(s) 515
PpsI GAGTC 1 cut(s) 515
Psp6I CCWGG 1 cut(s) 222
PspGI CCWGG 1 cut(s) 222
PspPI GGNCC 2 cut(s) 34, 80
RsaI GTAC 1 cut(s) 644
RsaNI GTAC 1 cut(s) 643
RseI CAYNNNNRTG 1 cut(s) 471
SacII CCGCGG 2 cut(s) 517, 530
SapI GCTCTTC 1 cut(s) 542
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 3 cut(s) 144, 309, 385
Sau3AI GATC 3 cut(s) 462, 593, 678
Sau96I GGNCC 2 cut(s) 34, 80
ScaI AGTACT 1 cut(s) 644
SchI GAGTC 1 cut(s) 515
ScrFI CCNGG 1 cut(s) 224
SetI ASST 6 cut(s) 39, 104, 367, 386, 495, 655
SfcI CTRYAG 1 cut(s) 613
Sfr303I CCGCGG 2 cut(s) 517, 530
SgrBI CCGCGG 2 cut(s) 517, 530
SinI GGWCC 1 cut(s) 34
SmiMI CAYNNNNRTG 1 cut(s) 471
Sse9I AATT 3 cut(s) 291, 573, 619
SseBI AGGCCT 1 cut(s) 484
SsiI CCGC 8 cut(s) 93, 132, 144, 148, 514, 516, 527, 529
SspMI CTAG 1 cut(s) 671
StuI AGGCCT 1 cut(s) 484
StyD4I CCNGG 1 cut(s) 222
TaqI TCGA 2 cut(s) 351, 560
TasI AATT 3 cut(s) 291, 573, 619
TatI WGTACW 1 cut(s) 642
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 3 cut(s) 233, 661, 695
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 2 cut(s) 444, 688
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 2 cut(s) 308, 384
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 8 cut(s) 166, 225, 281, 305, 390, 489, 529, 589
TspRI CASTG 2 cut(s) 444, 688
VpaK11BI GGWCC 1 cut(s) 34
XcmI CCANNNNNNNNNTGG 1 cut(s) 395
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 1 cut(s) 671
ZrmI AGTACT 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.